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Utilizing Hemoglobin Binding Kinetics And X-Ray Crystallography In Sickle Cell Disease Drug Development, Ahmed Alghamdi

Theses and Dissertations

ABSTRACT

UTILIZING HEMOGLOBIN BINDING KINETICS AND X-RAY CRYSTALLOGRAPHY IN SICKLE CELL DISEASE DRUG DEVELOPMENT

By Ahmed Alghamdi, M.Sc. in Medicinal Chemistry

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Medicinal Chemistry at Virginia Commonwealth University.

Virginia Commonwealth University, 2024.

Director: Dr. Martin K. Safo Professor, Department of Medicinal Chemistry

Introduction: Sickle Cell Disease (SCD) is characterized by severe hemolytic anemia, recurrent painful crises, and chronic organ damage, posing significant challenges to patients' quality of life and longevity. While current FDA-approved therapies offer symptomatic relief, they do not address the underlying molecular …

The Patient Experience Of Language Discordance And Interpreter Services In The Emergency Department, Keri L. Baker

Theses and Dissertations

Background: Clear communication is critical to the interaction of a patient and medical provider. For non-English speaking patients, language discordance, described as the lack of proficiency in a shared language, with the medical team results in a poor patient experience and poor patient outcomes. The predominant solution to language discordance is the use of interpreter services, however these services are underused in the emergency department (ED). Studies examining underuse of interpreter services have primarily focused on the clinician and little is known about the patient’s influence on the use of interpreter services.

Methods: Semi-structured interviews were conducted with 12 Spanish-speaking …

Continuous Flow As A Process Intensification Tool For The Preparation Of Pharmaceutically Relevant N-Heterocycles, John W. Tomlin Jr

Theses and Dissertations

Continuous flow as a process intensification tool for the preparation of pharmaceutically relevant N-heterocycles

John W. Tomlin

Thesis Defense: 17 January 2024

Committee: B. Frank Gupton, Ph.D. (Advisor), Suzanne M. Ruder, Ph.D. (Chair), Heather R. Lucas, Ph.D., James K. Ferri, Ph.D., Matthew C.T. Hartman, Ph.D.

A dissertation submitted in partial fulfillment of the requirements of Doctor of Philosophy at Virginia Commonwealth University

© 2024 John W. Tomlin Jr All Rights Reserved

Abstract

Essential medicines must be produced in large quantities to accommodate large global demand. The preparation of these active pharmaceutical ingredients (API) must therefore strive to be both cost-effective …

Graph Coloring Reconfiguration, Reem Mahmoud

Theses and Dissertations

Reconfiguration is the concept of moving between different solutions to a problem by transforming one solution into another using some prescribed transformation rule (move). Given two solutions s₁ and s₂ of a problem, reconfiguration asks whether there exists a sequence of moves which transforms s₁ into s₂. Reconfiguration is an area of research with many contributions towards various fields such as mathematics and computer science.
The k-coloring reconfiguration problem asks whether there exists a sequence of moves which transforms one k-coloring of a graph G into another. A move in this case is a type …

Mechanistic Analysis Of Four-Way Dna Junctions And Cytokine-Binding Aptamers For Therapeutic Interventions, Roaa S. Mahmoud

Theses and Dissertations

DNA is inherently dynamic and topologically diverse and can fold into many different structures. Besides the canonical Watson-Crick structure, other higher-order structures such as G-quadruplexes (G4), i-motifs (iM), and four-way DNA junctions are possible. Although these high-order DNA structures are known to form transiently, they are important due to the crucial roles they play in many cellular processes including DNA replication, recombination, and repair. Among these DNA structures, 4-way junctions (also known as Holliday junctions, HJ) which are formed during the repair of double-strand DNA breaks (DSBs) and interact with proteins have garnered significant attention due to their central role …

Redesign, Evaluation, And Validation Of A Commercially Viable High-Resolution Melt Based Mixture Screening Tool, Chastyn Smith

Theses and Dissertations

Analysis of evidentiary samples containing DNA from multiple contributors (“mixtures”) is a time intensive process for a forensic analyst and one where the contributor nature of a sample is not revealed until the end of the traditional forensic workflow. Often, at this stage, retesting or additional testing of mixture samples may not be possible, particularly if the DNA collection device did not preserve the DNA well enough; consequently leaving only trace amounts of a contributor’s DNA present. Thus, a new collection device that would allow for the increased preservation/integrity of evidentiary samples as well as a method that would allow …

Polyvictimization In Context: Identifying Patterns Of Peer, Dating, And Community Violence Among Black Early Adolescents, Carine Leslie

Theses and Dissertations

Early adolescents exposed to multiple forms of victimization (“polyvictimization”) may experience a number of detrimental psychosocial outcomes. Some person-centered research has identified victimization subgroups among adolescents but have not examined the overlap of peer, dating, and community victimization among Black early adolescents living in economically disadvantaged urban communities. Thus, this study empirically identifies victimization profiles in a sample of Black early adolescents to ascertain the patterns and correlates of polyvictimization. The present study aimed to (a) identify subgroups of Black early adolescents based on their frequency of peer, dating, and community victimization using latent profile analyses, and (b) evaluate the …

Facilitative And Competitive Interactions Among Dune Grass Species In Monoculture And Polyculture Communities, Anne M. Sciolino

Theses and Dissertations

Coastal dunes are natural barriers between land and sea and are imperative to the protection and preservation of inland ecosystems and human infrastructure. However, due to global warming, seal level rise, and coastal development, coastal dunes are under constant threat of destruction. Restoration efforts have been implemented to rebuild the protective function of dunes through the planting of dune building grasses. Dune grasses build and maintain these vital sand dunes. However, little is known about facilitative and competitive relationships among the grasses. In order to maximize restoration efforts, research is needed to better understand the biotic interactions among restorative grass …

External Runtime Execution Monitoring Of A Cyber Physical System Via Trace Interfaces, Peter Vaughan Truslow

Theses and Dissertations

In the past two decades, Unmanned Aerial Systems have progressed from expensive military hardware or one-off custom builds, to include off-the-shelf drones that can be purchased for a rather affordable price and flown by nearly anyone. As the technology and performance have improved, the door is opened to applications that require operation in environments where the consequences for failure are high, such as operating in the navigable airspace or in urban environments, or with human passengers. This requires a great deal of trust in the reliability and integrity of the control systems of the aircraft. A method of monitoring the …

Repeated-Measures Latent Profile Analyses Of Intervention Fidelity To A Teacher-Delivered Intervention Program For Children At Elevated Risk For Emotional And Behavioral Disorders, Katrina A. Markowicz

Theses and Dissertations

Teachers have difficulty delivering Tier-2 intervention programs for preschoolers at elevated risk for socioemotional and behavioral disorders with high intervention fidelity (Durlak, 2010; Sutherland et al., 2018). Several proximal factors that impact program fidelity have been identified, which may undermine program effects (Domitrovich et al., 2008; McLeod et al., 2022; Sutherland et al., 2018). Therefore, researchers have identified strategies, such as practice-based coaching, to improve intervention fidelity and support teachers (Hemmeter et al., 2015; Synder et al., 2015). Most of the field's knowledge of these topics is limited to variable-centered approaches (Fettig et al., 2022; Low et al., 2016). However, …

Correlates Of Coffee And Energy Drink Use Among Individuals With Severe Alcohol Use Disorder, Enkelejda Ngjelina

Theses and Dissertations

Research on caffeine consumption among college students and young adults has primarily focused on caffeinated energy drinks (EDs) and their combination with alcohol, demonstrating a strong association with heavy and problematic alcohol use. However, little attention has been directed toward older adults. Furthermore, studies have shown that regular ED consumers are more likely to meet the criteria for alcohol dependence compared to their peers. Despite this association, little is known about ED consumption among individuals with Alcohol Use Disorder (AUD), particularly those undergoing treatment, and even less is understood about other caffeine sources such as coffee. This study aimed to …

Systemic Contributors To Health Equity: A Conceptual Framework And Moderation Analysis, Rachel Regal

Theses and Dissertations

This dissertation presents a line of research exploring systemic factors that influence health and health equity across the lifespan. A linking document prefaces two-manuscripts as follows. Manuscript 1, A Model for Enhanced Trauma-Informed Care: An Asset-Based, Transdisciplinary Approach to Health Equity, presents a synthesis of literature on multisystem resilience, social determinants of health, and adverse / positive childhood experiences to build an alternative framework to promote healing and health equity using an asset-based approach within enhanced trauma-informed systems of care and educational models. By adopting this enhanced framework, the counseling field and others can be responsive to emerging trends and …

Roleplaying Builds Character: Avatar Performance In Dungeons & Dragons As An Art-Form For Self-Development., Maria F. Pagola Garcia

Theses and Dissertations

This paper examines the impact of the performance and embodiment of Dungeons & Dragons characters on their player’s self-development and self-confidence. Focusing on the game’s 5th edition, this narrative inquiry study is concerned with the applications of D&D as an artistic tool to build both community in formal and informal spaces of art education and individual players’ self-development through the avatars being portrayed during game time.

Ten adult participants ranging from age 23-28 were selected using a mix of direct recruitment and voluntary response. They filled out a digital survey introducing themselves and their main D&D characters, then a 45-minute …

The Genetic Architecture Of Cervical Change During Pregnancy: From Modeling To Mechanism — Does The Cervix Mediate Maternal Risk For Spontaneous Preterm Birth?, Hope M. Wolf

Theses and Dissertations

This project leverages clinical data and biospecimens from a prospective longitudinal cohort of pregnant women to study the genetic and phenotypic relationships between cervical shortening and the duration of pregnancy. Sonographic cervical length (CL) was measured throughout pregnancy in a cohort of 5,160 Black/African American women in Detroit, Michigan. Maternal DNA samples were sequenced with a next-generation low-pass whole genome platform. The heritability of cervical change during pregnancy and its genetic correlation with gestational age at delivery (GAD) were estimated using Genome-Wide Complex Trait Analysis. These estimates suggest that cervical change is heritable (h²CL = 51%) and highly polygenic trait. …

Feasibility Of Using Somatosensory Input On Pediatric Patients Receiving Dental Treatment, Liara D. Vinson

Theses and Dissertations

Purpose: The aim of this randomized, cross-over designed feasibility study is to evaluate patient anxiety, patient cooperation, and patient acceptance of a vibrating weighted vest used during the restorative treatment compared to when no device is used.

Methods: Children aged 5 to 15 years, needing at least two appointments for restorative treatment requiring local anesthesia under basic behavior guidance techniques were recruited. Patients were randomly allocated to wearing a vibrating weighted vest at one of the appointments. At both appointments, behavior was evaluated using Frankl scores and anxiety was evaluated at the beginning and at the end of …

Towards The Automation Of Post-Processing Of Additive Manufacturing Using Collaborative Robots, Logan Schorr

Theses and Dissertations

Despite the incredible potential that collaborative robots have, they are underutilized in several industries that could benefit greatly. These specialized fields, such as additive manufacturing, face challenges that can be solved with the use of robotics, including advanced computer vision, end-effector tooling, and process refinement. My research aims to utilize collaborative robots in an effort to solve the above stated problems, for the specific fields of additive manufacturing. Specifically within this field is the automation of post-processing; this is normally completely manual, as the tools for automation are not sufficiently developed to handle the flexibility required for additive manufacturing. This …

Development And Evaluation Of Reactor Physics Transient Benchmarks For Molten Salt Reactors Based On The Molten Salt Reactor Experiment, Mohamed H. Elhareef

Theses and Dissertations

Molten salt reactors (MSRs) are a promising class of advanced nuclear reactors selected as

one of the generation IV reactors. This class of reactors employs a unique design, where fissile

material is dissolved in a molten salt mixture, serving as fuel and coolant of the reactor. The design

features high-temperature low-pressure operation and efficient heat transfer capability, which

leads to significant improvements in reactor thermal efficiency, safety, and waste management.

However, MSRs also have complex phenomena not observed in conventional reactors, such as

fission product transport in the circulating fuel loop. These phenomena impact neutron distribution

and reactivity feedback mechanisms …

Impact Of Environmental, Social, And Governance Disclosure On Firm Performance: A Case Of Listed Manufacturing Firms In Nigeria, Abdulbaki Ubandawaki

Theses and Dissertations

The traditional goal of companies is to maximize shareholders’ wealth. However, to achieve this objective, many complementary goals must be pursued alongside the traditional ones. To achieve corporate goals, businesses need to interact with the environment. The continual interaction of the corporation with the environment has definitely come with its costs and benefits, and the global interest in promoting sustainable development has made corporate ESG reporting a crucial issue. The aim of the study is to investigate the relationship between ESG reporting and firm performance among listed manufacturing firms in Nigeria. The data was collected from annual and standalone sustainability …

Putting The “Proof” In The Pudding: Ingredients To Consider In Executing An Effective And Sustainable Diversity Management Strategy Within Esg Conscious Organizations, Diamond D. Dubose

Theses and Dissertations

ESG exchange-trade fund inflows increased to a total of 22 billion dollars in 2020; three times their amount as compared to 2019, reflecting increasing capital market prominence (Wagner & Ballentine, 2020). Many organizations have since engaged in DEI practices because of the appeal Environment, Social, and Governance (also known as ESG) investment ratings provide (Henisz et al., 2019). However, companies must go beyond publishing organizational commitments to diversity to ‘check the box’ if they want to remain in the good graces of their internal and external stakeholders, ultimately validating their financial performance (S. J. Singh, 2021). An obstacle also emerged …

A Quantitative Analysis Of Empathy Trait As A Moderator Of Leadership Behavior, Barbara N. Rodriguez

Theses and Dissertations

There are indications that empathic concern for others has been declining around the world (Konrath et al., 2014). Empathy has long been recognized as a component of social functioning that significantly influences behavior that impacts relationships with others on both personal and societal levels (Batson, 2009; Davis, 2017; M. L. Hoffman, 2000; Stilwell, 2001). Leaders who apply empathy in their leadership can promote stronger connections with others (Gentry & Sadri, 2007), and foster social closeness and well-being (Morelli et al., 2015). While traits and behaviors are helpful predictors of effective leadership (Judge & Piccolo, 2004; Mumford et al., 2000), there …

Is It In The Job Description? Best Practices For School Psychologists’ Advocacy For Systemic Change Benefiting Black Students, Taylor Manuel Moore

Theses and Dissertations

School psychologists can be essential team members in driving systemic change towards achieving educational fairness, as their experiences, perspectives, and actions significantly contribute to this effort. This qualitative phenomenological study delved into an in-depth investigation of the lived experiences of school psychologists who have committed themselves to advocating for transformative changes that have a direct influence on the educational paths of Black students. The research design entailed conducting in-depth interviews with practicing school psychologists who have actively engaged in advocacy campaigns. The objective of phenomenological inquiry was to reveal the fundamental nature of individuals' experiences, providing insight into the obstacles …

Perceptions Of Classroom Technology Use Among Adult English Learners, Mandy Chien

Theses and Dissertations

The purpose of this study was to assess adult English Learners’ perceptions of using Microsoft Teams in classes. Specifically, the study aims to explore the strength and direction of the correlation between the elements of the technology acceptance model (TAM) and using Microsoft Teams in English language courses. This study addressed the following research question: What are the strengths and directions of the correlations between the elements of the technology acceptance model and the use of Microsoft Teams among adult ELs?

The researcher utilized a quantitative methods approach to investigate the relationship between TAM variables and the use of Microsoft …

Certified Impact: Organization Design For Zero Waste Certification And Its Impact On Organizational Identity, Sumner Byrne

Theses and Dissertations

This phenomenological study examined how organizations, primarily in the manufacturing and distribution industries, approached the change process to achieve a third-party verified zero waste certification. It investigated how the change process impacted employees’ sense of organizational identity, and what challenges they faced in pursuing certification. Sixteen participants from seven recently certified organizations agreed to be interviewed about their experience. Challenges stemmed most consistently from fiscal misalignment with leadership’s priorities, capacity struggles, and employee disconnection to the certification’s purpose. Participants reported that the most impactful organizational change strategies included clear, positive messaging, investment in employee empowerment and leadership, and consistency throughout …

Fashion Education And Training Prevalence In Fashion Career Success: An Analysis, Mankirat Kaur

Theses and Dissertations

Is the acquisition of fashion education and fashion training for career success a compulsion or a choice? Many designers came and left their legacies to inspire future generations, yet their journeys have often been overlooked in academic research. The purpose of this study is to investigate the impact of education and training through core facets of lifelong learning —formal, non-formal, and informal education —over the past century on achieving career success in the fashion industry. A mixed-method content analysis, utilizing triangulation, was conducted to understand the educational and training experiences of prominent designers. Two research questions guided the analysis: RQ1 …

Characterization Of Tonneau1 Recruiting Motif Proteins And Ton2 Signaling Functions, Tyler Falk

Theses and Dissertations

Plants cells are fixed in place. As a result they have to carefully divide in order to function properly. Plants use a number of complex microtubule arrays to ensure proper division and orientation including the preprophase band (PPB) and the phragmoplast. The PPB orients the cell prior to division and marks the cortical division site, which is where the phragmoplast is guided to. The phragmoplast is a dynamic microtubule array that builds the cell plate which later becomes the cell wall that divides daughter cells. Proteins involved with PPB formation include Tonneau1 (TON1) and Tonneau2 (TON2) which localize to and …

Food For Thought: Fabricating The Cannibal In Colonization, Hannah Dubrovich

Theses and Dissertations

Anthropophagy means people-eating, as does cannibal, but the terms are not synonymous. After Europeans landed in the Americas, Christopher Columbus coined the word cannibal. Despite the two terms having similar base definitions, there are additional connotations associated with cannibal. The way in which cannibalism has been thought about by Western cultures is not about the act of people-eating, but the constructed character of the cannibal as a “savage” Other created through colonization. In addition to the term's associated meaning being cemented during this time, colonizers used the term as a tool to Other Native peoples and aid in colonization. It …

Social Networks As Predictors Of Musculoskeletal Injury In U.S. Army Soldiers (Snap-Mski), Kristen L. Zosel

Theses and Dissertations

INTRODUCTION: Musculoskeletal injuries (MSKIs) are the leading cause of medical non-readiness among U.S. Army Soldiers, costing the Military Health System an estimated $4.7 billion annually in direct and indirect costs. Current MSKI mitigation efforts focus on individual attributes such as body mass index and gender. However, emerging research highlights the significance of social ties on health outcomes and behaviors. This study examined the patterns of MSKI within military social networks and how social interactions, along with health behaviors such as sleep, physical activity (PA), and diet, co-occur in Soldiers with and without MSKI. METHODS: Phase 1 involved a cross-sectional sociocentric …

Terror And Time: Analyzing Homegrown Terrorism In The United States, Kyser Daniels-Baessler

Theses and Dissertations

The events of September 11th, 2001, prompted a global reckoning with the threat of international terrorism, leading to intensified awareness and policy responses worldwide. However, amidst this heightened global focus, the United States has faced a concerning rise in politically motivated violence on its soil. Against this backdrop, this thesis delves into the phenomenon of homegrown terrorism within the United States from 2000 to 2020. By employing advanced geospatial techniques, this study seeks to pinpoint areas nationwide with elevated rates of homegrown terrorist incidents. Moreover, it endeavors to uncover potential temporal correlations between these acts of violence and significant events, …

The Leadership Blind Spot: Why Are Black Boys Missing From The School’S Leadership Positions?, Chima Ikeme

Theses and Dissertations

The purpose of this phenomenological action research study was to explore the factors that have contributed to the absence of Black boys in school leadership positions. The study looked to answer the following research questions: How do Black male students perceive leadership and their connection to leadership roles on campus? What barriers exist that discourage or prohibit Black boys from engaging in school organization student leadership positions? How can student leadership from Black boys on campus be increased?Critical Race Theory, Transformational Leadership Theory, and Self Determination Theory served as the theoretical frameworks that grounded this study. Utilizing a qualitative approach …

Linking Ethical Leadership To Organizational Citizenship Behavior And Unethical Pro-Organizational Behavior, Jinhee Kim

Theses and Dissertations

The significance of ethical leadership has recently garnered considerable attention from both academia and practitioners. However, despite its reputation for facilitating ethical conduct and preventing unethical conduct, there are inconsistencies in the findings about its relationship with (un)ethical pro-organizational behaviors such as organizational citizenship behavior (OCB) and unethical pro-organizational behavior (UPB). Thus, the purpose of this dissertation is to delve deeply into the influence of ethical leadership on OCB and UPB. Through three interrelated papers, I seek insights into how ethical leadership can drive ethical pro-organizational behaviors that are compatible with broader societal norms. In Paper 1, while noting a …