The Role Of Learning Experiences, Supports And Barriers In Career Development For The Doctoral Student, Madeline B. Goldman

Theses and Dissertations

Abstract

THE ROLE OF LEARNING EXPERIENCES, SUPPORTS AND BARRIERS FOR THE DOCTORAL STUDENT

By Madeline B. Goldman, DDS, MEd

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University.

Virginia Commonwealth University, 2018

Major Director: Robin Hurst, Ed.D., Assistant Professor, Department of Teaching and Learning

This study seeks to understand the role of career development classes offered by the graduate school at a large public research university as part of its Leadership and Entrepreneurs for Professional Development (LEAPD) program and the LEAPD program effects on doctoral students’ career development and …

Resolution Of Proximal Oxidative Base Damage And 3′-Phosphate Termini For Nonhomologous End Joining Of Free Radical-Mediated Dna Double-Strand Breaks, Sri Lakshmi Chalasani

Theses and Dissertations

Clustered damage to DNA is a signature mark of radiation-induced damage, which involves damage to the nucleobases and/or DNA backbone. Double-strand breaks created by damaging agents are detrimental to cell survival leading to chromosomal translocations. Normal cells employ Non-homologous end-joining because of its faster kinetics, to suppress chromosomal translocations. However, the presence of complex DNA ends constitutes a significant challenge to NHEJ. Location of Thymine glycol (Tg) at DSB ends was a potential hindrance to end joining. The substrate with Tg at the third position (Tg3) from the DSB joined better than when present at the fifth position (Tg5). However, …

Forest Structural Complexity And Net Primary Production Resilience Across A Gradient Of Disturbance In A Great Lakes Ecosystem, Lisa T. Haber

Theses and Dissertations

Forests are an important component of the global carbon (C) cycle and contribute to climate change mitigation through atmospheric C uptake and storage in biomass and soils. However, the forest C sink is susceptible to disturbance, which modifies physical and biological structure and limits spatial extent of forests. Unlike severe, stand-replacing disturbances that reset forest successional trajectories and may simplify ecosystem structure, moderate severity disturbances may instead introduce complexity in ways that sustain net primary production (NPP), leading to the phenomenon of “NPP resilience.” In this study, we examined the linkage between disturbance severity and ecosystem biological and physical structural …

Ageism Among Healthcare Professionals: The Influence Of Personal Aging Anxiety, Job Role, And Work Setting On Attitudes Toward Older Patients, Jennifer L. K. Inker

Theses and Dissertations

Older adults make up a significant and increasing proportion of the U.S. population and are frequent users of healthcare services. Ageism in healthcare, driven by an incomplete and narrowly biomedical perspective on aging, has been linked to various problematic outcomes for older patients, including under- and over-treatment. The purpose of this study was to use the theory of relational ageism to explore the relationship between personal aging anxiety among healthcare professionals and their attitudes to older patients, considering the potentially moderating factors of job role and work setting. Using convenience sampling, clinical healthcare professionals working for a mid-sized, regional healthcare …

Usefulness Of The Captia Syphilis Igg Eia Test Method And Reverse Algorithm For Detection Of Syphilis Infection In A Public Health Setting, Patricia Armour

Theses and Dissertations

Syphilis, a systemic sexually transmitted disease, is on the rise in the US, with infection rates the highest recorded since 1994 according to the CDC. Useful laboratory testing is an important diagnostic tool for determining individual syphilis infection and preventing community-wide disease spread.

The purpose of this study was to determine the usefulness of a specific automated treponemal test method, the Captia^TM Syphilis IgG EIA, and the syphilis reverse algorithm interpretation for detecting syphilis infection among patients seeking care in a public health clinic. The study employed a retrospective, nonexperimental descriptive correlational design with data collected between 2012-2013 from …

Mechanisms Regulating Axon Initial Segment Stability, Savannah D. Benusa

Theses and Dissertations

Axon initial segment (AIS) disruption has been described in a number of pathological environments where neuroinflammation is a contributing factor; however, whether this disruption is reversible in unknown. To address the principle of AIS structural recovery, we employed an acute neuroinflammatory model. Acute neuroinflammation induced disruption of AIS structural and functional domains and, importantly, upon resolution of neuroinflammatory conditions, was reversed.

Consistent with other studies, we observed a close interaction of microglia with AISs, and utilized this acute neuroinflammatory model to investigate the relationship between reactive microglia and AIS integrity. Gene expression analysis of microglial transcription profiles identified reactive oxygen …

Evolving Reproductive Isolation In The Parasitic Wasp Genus Cotesia, Justin P. Bredlau

Theses and Dissertations

Parasitic wasps are highly diverse and play a major role in suppression of herbivorous pest populations, but relatively little is known of the mechanisms driving their diversity. Molecular studies indicate that cryptic species complexes resulting from adaptations to specific hosts or host-foodplants may be common. The gregarious endoparasitoid, Cotesia congregata (Braconidae), is a model system for understanding parasitic wasp biology. It is reported to attack at least 15 species of sphingid caterpillars, most of which are plant family specialists. Molecular studies have demonstrated genetic differentiation of two host-foodplant complex sources originating from Manduca sexta on tobacco (MsT) and Ceratomia catalpae …

Synthesis And Analysis Of Gold Nanoclusters, Patrick Woodworth

Theses and Dissertations

Gold Nanoclusters are of particular interest due to their many possible applications across a wide range of scientific fields. More specifically, nano-sized gold particles have potential to be used in drug delivery systems, cancer therapy and catalysis. This dissertation focuses on improving our understanding of ligated gold nanoclusters by examining the role of a variety of phosphine based ligands, novel methods to produce monodisperse solutions, and investigating the kinetics of water soluble ligated gold nanoclusters.

The addition of ligands to solutions of Au have shown to produce small (< 20 Au atoms) clusters. All nanocluster solutions were prepared in a similar manner. Typically, a gold salt, either Chloro(triphenylphosphine) gold(I) (Au(PPh₃)Cl), or Potassium gold (III) chloride (KAuCl₄), were …

Using A Custom Landscape Classification To Understand The Factors Driving Site Occupancy By A Rapidly Declining Migratory Songbird, Elizabeth K. Schold

Theses and Dissertations

Land cover classifications are useful in a broad range of ecological applications, yet publicly available classifications are not always useful for the needs of specific projects. Custom classifications are always a possibility, however, they can be financially or computationally out of reach for many researchers. Here we present a custom 1m resolution land cover classification created using freely available imagery and a random forest classification approach. This classification detected shrub cover more accurately and at a finer resolution than previous classifications. With the creation of this map, we were then able to examine landscape factors influencing occupancy dynamics of the …

Assessment Of A Predicted Diguanylate Cyclase In Treponema Denticola, Dhara T. Patel

Theses and Dissertations

Periodontal disease is a progressive inflammatory condition that is characterized by the reabsorption of alveolar bone, the destruction of connective tissue, and edentulism. It is caused by a dysbiosis in the oral microbiome as a result of a shift from a Gram-positive aerobic bacterial population, to one that becomes more Gram-negative and anaerobic. Treponema denticola is thought to drive this diseased state based on its role as a keystone periopathogen. A major component of T.denticola’s invasiveness is its motility, which allows the spirochete to penetrate and disseminate through tissues. This motility, which has been seen to be crucial to …

Zebrafish Model Of Mll-Rearranged Acute Myeloid Leukemia, Alex J. Belt

Theses and Dissertations

Acute myeloid leukemia (AML) is the second most common type of leukemia and accounts for 80% of adult acute leukemia cases and is characterized by the accumulation of poorly or undifferentiated myeloid blast cells. Standard treatment includes chemotherapy, which if unsuccessful, is followed by more rigorous chemotherapy as well as stem cell transplantation. Considering most patients are over the age of 45, these more rigorous therapies are not always possible, and as such, new therapies must be developed. Furthermore, AML patients harboring a chromosomal rearrangement involving Multiple Lineage Leukemia (MLL) that results in the expression of an MLL fusion protein …

Understanding The Role Of Sport For Development In Addressing Health Disparities In Low-Ses Communities, Tiesha R. Martin

Theses and Dissertations

The purpose of this study was to understand the role of Sport for Development (SFD) in addressing health disparities in low-SES communities. This was done using a multiple case study design, in which administrators, staff, and youth participants from five SFD programs were interviewed. A theoretical model, consisting of the theory of fundamental causes (Link & Phelan, 1995), the classification of SFD programs (Coalter, 2007), and the ecological model of health promotion (McLeroy, 1988), was developed to guide this study.

Interviews were transcribed and then analyzed using a deductive coding process (Gilgun, 2005). The findings revealed that the SFD programs …

Women In Music: Letting A Long Story Be Long Contemplating Women’S Sonic, Musical, And Spiritual Experiences In Prehistory, Deborah J. Saidel

Theses and Dissertations

Situated within deep history, this study explores the auditory and spiritual lives of Paleolithic women. It considers their personal agency in mediating the spiritual power of sound and how doing so contributes to a multifaceted musicality. The theoretical framework involves a wide spectrum of topics, from ways of rethinking the writing of history and reckoning with time, to sound studies and the study of acoustics in ancient sites, to a critical examination through a feminist lens of normative disciplinary scholarship in anthropology and archaeology, religious studies, and musicology. I explore potential audio-visual-lithic relationships for their implications for deepening an understanding …

Examination Of The Role Of Dehumanization As A Potential Mechanism Underlying The Racial Disparities In School Disciplinary Measures., Ebony A. Lambert

Theses and Dissertations

Schools should be safe and supportive spaces for all students, yet Black students tend to face biased treatment in the education system, which often results in harsh disciplinary measures. This research examined the role of animalistic dehumanization (i.e., perceiving others as animal-like and uncultured and denying uniquely human characteristics), in predicting choice of harsher disciplinary measures for Black students as opposed to White students. It was hypothesized that individuals who dehumanize Black students to a greater degree would be more likely to believe that Black students need to be disciplined through harsher measures. Additionally, it was hypothesized that the link …

Red-Headed Woodpecker Full Annual Cycle Ecology At Fort A.P. Hill, Virginia, Benjamin Nickley

Theses and Dissertations

The red-headed woodpecker is a primary cavity excavator found throughout the Eastern Deciduous Forest and parts of the Great Plains. Although widespread, the red-headed woodpecker is generally considered uncommon, with patchily distributed populations. Over the past 50 years, this species has experienced precipitous, range-wide declines. The red-headed woodpecker uses a variety of cover types to meet resource needs across the annual cycle, ranging from oak savannas and wetlands, to mature beech forests and urban parks. Given their apparent adaptability to such a wide range of habitats, causes of declines are perplexing. To understand and stem declines, recent studies have focused …

Shared Genetic And Environmental Influences On Fear, Anxiety, Posttraumatic Stress, And Brain Morphometry, Chelsea Sawyers

Theses and Dissertations

Anxiety disorders (ADs) and stress-related disorders are some of the most common psychiatric disorders in the United States. Like other c0mplex psychiatric illness, genetics and neuroimaging research has focused on understanding their underlying neurobiology. Areas within the fear-network play important roles in threat perception, fear conditioning/learning, cognitive processing, and modulation of fear responses including contextual modulation and extinction and have been implicated in ADs as well as stress disorders such as posttraumatic stress disorder (PTSD). The primary gap in the current search for underlying biological mechanisms is in whether biomarkers associated with disorders share genetic influences with the disorders they …

Mechanisms Of B-Myb Oncogenicity In Ovarian Cancer, Audra N. Iness

Theses and Dissertations

High expression of B-Myb (encoded by MYBL2), an oncogenic transcription factor, is associated with cell cycle deregulation and poor prognosis in several cancers, including ovarian cancer. However, the mechanism by which B-Myb alters the cell cycle is not fully understood. In proliferating cells, B-Myb interacts with the MuvB core complex including LIN9, LIN37, LIN52, RBBP4, and LIN54, forming the MMB (Myb-MuvB) complex, and promotes transcription of genes required for mitosis. Alternatively, the MuvB core interacts with Rb-like protein p130 and E2F4-DP1 to form the DREAM complex that mediates global repression of cell cycle genes in G0/G1, including a subset …

5-Ht2b Receptor-Mediated Cardiac Valvulopathy, Pallavi Nistala

Theses and Dissertations

5-HT_2B receptor agonism causes cardiac valvulopathy, a condition characterized by thickening of the heart valves and as a result, regurgitation of blood within the heart. The anti-obesity drug fenfluramine, which was originally prescribed as an anorectic, was withdrawn from the market due to causing cardiac valvulopathy. Fenfluramine, after metabolism by N-dealkylation, produces the metabolite norfenfluramine, which acts as a more potent valvulopathogen. The same was seen with MDMA (ecstasy), a popular drug of abuse, which is metabolized by N-dealkylation to produce MDA, a more potent valvulopathogen. Glennon and co-workers. studied a series of 2,5-dimethoxy-4- substituted phenylisopropylamines (DOX type) hallucinogens …

Decellularized Matrices Effect On The Adaptive Immune Response, Kegan Sowers

Theses and Dissertations

Decellularized extracellular matrices have been a growing area of interest in the biomedical engineering fields of tissue engineering and regenerative medicine.As these materials move toward clinical applications, the immune response to these materials will be a driving force toward their success in clinical approaches. Fully digested decellularized matrix constructs derived from porcine liver, muscle and lung were created to test the adaptive immune response. Hydrogel characterization ensured that the materials had relatively similar stiffness levels to reduce variability, and in vitro studies were conducted. Each individual construct as well as a gelatin control were plated with a co-culture of macrophages …

The Effects Of Stand-Alone Prescription Drug Plans On Health Care Utilizations, Expenditures And Medication Adherence Among Elderly Medicare Beneficiaries, Jing Yuan

Theses and Dissertations

Since the implementation of Medicare Part D in 2006, a growing body of evidence has shown that Medicare Part D might offset the total healthcare spending by improving the use of prescription drugs. However, little is known about the impact of different types of Part D plans – Stand-alone Prescription Drug Plan (PDPs) and Medicare Advantage Prescription Drug Plan (MA-PDs) – on health care utilizations and expenditures. This dissertation examined the association between the effect in PDPs on health care utilizations as well as expenditures, and medication adherence among elderly Medicare beneficiaries, compared to MA-PDs. Data was pooled from 2006-2010 …

Regulation Of Pituitary Progenitor Differentiation By Β-Catenin, Julie Leann Youngblood

Theses and Dissertations

The pituitary gland is a critical organ that is necessary for many physiological processes, including growth, reproduction, and stress response. These vital processes are regulated through the secretion of pituitary hormones from specialized cell types. Pituitary hormone-producing cells arise from a common pool of pituitary progenitors in the developing Rathke’s pouch, and mutations that disrupt the formation and differentiation of pituitary progenitors often result in hypopituitarism, pituitary adenomas, or craniopharyngiomas. Thus, it is necessary to determine the mechanisms of pituitary development in order to understand the ways in which these congenital defects and tumors develop and disrupt pituitary function. Canonical …

Discrimination, Mental Health, And Preparedness For Aging In Trans(Gender)/Gender-Nonconforming Adults, Richard S. Henry

Theses and Dissertations

This cross-sectional study examined relationships among discrimination, mental health (i.e., depression and anxiety), preparation for aging (i.e., familiarity and planning), social support, death attitudes, and aging anxiety among TGNC adults (N = 154). Neither discrimination nor mental health predicted preparation for aging familiarity or planning. Discrimination did, however, predict both anxiety and depression, although only the non-affirmation subscale was a unique predictor of both. As discrimination and mental health were not a significant predictor of preparedness for aging in the previous regressions, the hypothesized mediation model and subsequent moderated mediation models were not conducted. Additional exploratory multiple regressions were …

Mechanistic Studies Of Proton-Coupled Electron Transfer Reactions Involving Antioxidants, Kejie Meng

Theses and Dissertations

The objective of the research was to investigate proton-coupled electron transfer (PCET) reactions involving antioxidants to gain insight into the detailed mechanisms of glutathione (GSH), Trolox, and α-tocopherol (α-TOH). PCET reactions are complex redox reactions that transfer electrons and protons sequentially or in concert. These reactions are ubiquitous in natural or artificial processes that produce electrochemical energy that is extractable as electricity or as chemical fuels of high energy content. Examples of processes based on PCET are photosynthesis, respiration, nitrogen fixation, carbon dioxide reduction, redox fuel cells, and artificial photosynthesis. Antioxidants were selected as a PCET model to understand the …

A Bronchiolitis Study: The Overuse Of Bronchiolitis Therapies In Children Admitted Into The Hospital, Jocelyn S. Canedo

Theses and Dissertations

Bronchiolitis is prevalent among infants of 0 to 12 months, and usually caused by a variation of viruses. Signs and symptoms consist of coughing, rapid breathing, and an occasional fever in some infants. Bronchiolitis is not treated with antibiotics, but rather with proper medical examination and other procedures. Chest radiographs, steroids, and certain bronchodilators are considered ineffective treatments. This study identified the amount of ineffective procedures being used in infants admitted into the pediatric emergency room, and the effect it had on the patient’s average hospital stay. Past medical records were used in this clinical study. Statistical analyses and graphs …

Improving Estuarine Water Quality In South Florida: A Quantitative Evaluation Of The Efficacy Of A Local Nutrient Ordinance, Kerstin Motsch

Theses and Dissertations

Degraded coastal water quality is a concern in Florida, in part due to nutrient enrichment of aquatic ecosystems causing eutrophication and excessive algal growth. In 2010, a fertilizer ordinance was enacted in Cape Coral located in Lee County Florida with the objective of reducing nutrient loads from local fertilizing practices in order to improve water quality within the city. In order to assess its efficacy, a before after control impact (BACI) design was implemented using Fort Lauderdale in Broward County Florida as a reference location. A total of twenty estuarine canal sampling locations were identified in both Lee and Broward …

Antibiotic Effects Of Dimethyl Sulfoxide And Synthetic Polyphenols On E. Coli Cells, Camila Cardenas

Theses and Dissertations

There is increasing interest in polyphenols due to their potential medicinal and antibiotic applications. Naturally occurring polyphenols, such as curcumin, have garnered tremendous attention. However, synthetic polyphenols commonly used in biochemistry experiments have not been sufficiently explored. The purpose of this study is to determine the effect of dimethyl sulfoxide on the cellular uptake of synthetic polyphenols. Three synthetic polyphenols (Phenol Red, Congo Red, and Bromophenol Blue) were tested for their antibiotic properties and compared to the antibiotic activity of curcumin. Our results show that phenol red does not interact with the bacterial membrane even in the presence of 30% …

Carbon Limitation In Periphytic Algal Wastewater Treatment Systems, Brandon J. Furnish

Theses and Dissertations

As global eutrophication poses an ever increasing threat to water quality new techniques must be implemented to improve the sustainability of natural resource consumption. Wastewater treatment facilities (WWTF) are designed to destroy pathogens, remove particulates, lower oxygen demanding substances, and reduce nutrients from influent waste water to avoid the degradation of receiving waters. WWTF are generally effective, however they are mostly inadequate at removing nutrients. When wastewater derived nutrients such as nitrate and phosphate are discharged to receiving waters, they stimulate algal blooms. Algal blooms can reduce dissolved oxygen, block sunlight for submerged aquatic plants, and render state waters unusable …

The Link Between Meditation And Effective Practice: Increasing The Pace Of The Acquisition Of Skill In Musical Practice, Caleb Cole

Theses and Dissertations

This thesis seeks to explore the idea of effective practice as being meditative in nature. Effective practice and meditation are both viewed independently, and elements of both are discussed. Meditation, in the literature, is viewed in different lights and is defined in many ways. This makes it difficult to nail down a single definition of meditation. The process itself is debated and several different categorizations are offered. Some research shows Unwanted Effects (UE) to meditation, but these concerns are not widespread. Meditation appears to be a healthy practice that may have health benefits. Effective practice is viewed next; Grit, deliberate …

An Investigation Of The Relationships Of Student Engagement And Academic Performance Of Supplemental Instruction Students Concurrently Enrolled In A Gateway Mathematics Course At California State University In Southern California, Keisha Renee Lee

Theses and Dissertations

This study, conducted at California State University (CSU) in Southern California, focused on student engagement factors and academic performance of supplemental instruction (SI) students concurrently enrolled in a gateway mathematics course. The purpose of this quantitative correlational survey study was to investigate engagement factors employed by SI students enrolled in gateway mathematics courses; the researcher explored the relationships of the SI students’ engagement factors to their gateway mathematics course grades. The participants completed a web-based survey in which they responded to items regarding their behaviors, thoughts, and feelings as experienced in the gateway mathematics course and the SI class sessions. …

Coping And Physical Well-Being Among First, 1.5, And Second-Generation Immigrants From Of Non-European Descent, Jacob R. Stein

Theses and Dissertations

This study brings attention to the growing body of literature examining the role of culture and context in the study of generation-status differences in cross-cultural coping and physical well-being among immigrants to the United States. Prior literature on the unique challenges, stressors, coping strategies, and health outcomes for immigrants provides a basis for hypothesized generation status differences on cross-cultural coping (collectivistic, avoidance, and engagement) and physical well-being (health, safety, and environmental). A sample of 118 male and female first, 1.5, and second-generation immigrants of non-European backgrounds, between the ages of 18 and 35, were recruited from the local community to …