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Crispr-Cas9 Transfection Optimization And Use In A Forward Genetic Screen To Identify Telomere Length Maintenance Genes, Kelsey Phillips Apr 2018

Crispr-Cas9 Transfection Optimization And Use In A Forward Genetic Screen To Identify Telomere Length Maintenance Genes, Kelsey Phillips

Theses and Dissertations

Mutations in the telomere length maintenance pathway can lead to a spectrum of diseases called telomere syndromes, however, the pathway is not fully understood and there may still be unknown components. We designed a forward genetic screen to identify new genes involved in telomere length maintenance. Of the top ranked genes, ZNF827, a zinc finger protein, is the most promising candidate gene. The possible discovery of a new component involved in telomere length maintenance increases our understanding of the pathway and opens new avenues of research. Recent advances in molecular biology techniques, such as the use of RNA-guided nuclease CRISPR …

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Emerging Seed Enhancements To Reduce The Risk Of Sagebrush Post-Fire Seeding Failure, Ryan Scott Call Apr 2018

Emerging Seed Enhancements To Reduce The Risk Of Sagebrush Post-Fire Seeding Failure, Ryan Scott Call

Theses and Dissertations

The sagebrush (Artemisia spp.) steppe is undergoing rapid ecological change. The degradation of sagebrush steppe rangelands has resulted in the listing of more than 350 animals and plants as species of conservation concern. In addition, there has been a decrease in recreational values, reduced forage production, degraded water resources, and an increase in fire frequency. In the sagebrush steppe, success rates for seeding sagebrush after wildfire are notoriously low. Not only are sagebrush seeds hard to sow due to their small size and associated flower parts, but seedlings are exposed to numerous stresses that lowers their survivability. To improve …

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The Evolutionary Significance Of Body Size In Burying Beetles, Ashlee Nichole Momcilovich Apr 2018

The Evolutionary Significance Of Body Size In Burying Beetles, Ashlee Nichole Momcilovich

Theses and Dissertations

Body size is one of the most commonly studied traits of an organism, which is largely due to its direct correlation with fitness, life history strategy, and physiology of the organism. Patterns of body size distribution are also often studied. The distribution of body size within species is looked at for suggestions of differential mating strategies or niche variation among ontogenetic development. Patterns are also examined among species to determine the effects of competition, environmental factors, and phylogenetic inertia. Finally, the distribution of body size across the geographic range of a species or group of closely related is looked at …

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Survival Of Neonate Mule Deer Fawns In Southern Utah: Effects Of Coyote Removal And Synchrony Of Parturition, Jacob Tyler Hall Apr 2018

Survival Of Neonate Mule Deer Fawns In Southern Utah: Effects Of Coyote Removal And Synchrony Of Parturition, Jacob Tyler Hall

Theses and Dissertations

Mule deer (Odocoileus hemionus) are an iconic species of wildlife, and populations of mule deer across much of the western U.S. have experienced recent fluctuations in size. Factors that affect the survival and subsequent recruitment of juveniles may be the preeminent cause of population fluctuations for mule deer in many areas. Many factors, including habitat loss, extreme weather, intense predation, timing and synchrony of parturition, and competition with other species may be influencing these changes. We studied two potential factors that can influence the survival of neonate mule deer in southern Utah. To better understand how predation affects …

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Measuring And Calculating Current Atmospheric Phosphorous And Nitrogen Loadings On Utah Lake Using Field Samples, Laboratory Methods, And Statistical Analysis: Implication For Water Quality Issues, Jacob Milton Olsen Apr 2018

Measuring And Calculating Current Atmospheric Phosphorous And Nitrogen Loadings On Utah Lake Using Field Samples, Laboratory Methods, And Statistical Analysis: Implication For Water Quality Issues, Jacob Milton Olsen

Theses and Dissertations

Atmospheric nutrient loading and transport though precipitation and dry deposition is one of the least understood yet one of the most important pathways of nutrient transport into many lakes. These nutrients, phosphorus and nitrogen, are essential for aquatic life and often play major roles in algae blooms that occur in lakes and reservoirs. Often heavy algal growth intensifies a variety of water quality problems. Utah Lake may be even more susceptible to atmospheric deposition due to its large surface area to volume ratio and proximity to Great Basin dust sources. In this study, eight months of atmospheric deposition data were …

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Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Neurons, Stephanie Bair Williams Apr 2018

Neuroimmune-Mediated Alcohol Effects On Ventral Tegmental Area Neurons, Stephanie Bair Williams

Theses and Dissertations

Dopamine (DA) transmission is a key player in the rewarding aspects of ethanol as well as ethanol dependence. The current dogma is that DA transmission is increased during ethanol via the inhibition of ventral tegmental area (VTA) GABA neurons and that excitation of VTA GABA neurons during withdrawal results in decreased DA transmission. Microglia, the major neuroimmune effector in the brain, may be a key mediator in this process by releasing cytokines following activation. We evaluated the effect of ethanol on cytokine concentrations in the VTA and NAc using a cytometric bead array, and found that low dose ethanol (1.0 …

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A Content-Analytic Meta-Analysis Of Gender Stereotyping In Screen Media, Lee Whitney Essig Apr 2018

A Content-Analytic Meta-Analysis Of Gender Stereotyping In Screen Media, Lee Whitney Essig

Theses and Dissertations

Thousands of studies have investigated the gendered portrayals in various forms of media. Researchers believe that considering gendered media content is important, as gender stereotypes have been found to influence development, often with adverse outcomes. Although a vast body of research on gender stereotypes has accumulated, little effort has been made to synthesize this literature. The purpose of this paper is to collect and analyze the results of content-analytic studies of gender portrayals across several forms of media including television, television commercials, movies, video games, music videos, and various forms of print media into a comprehensive paper on gender stereotypes …

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Creation And Use Of Software For Analysis Of Kinetic Proteomic Experiments, Bradley C. Naylor Apr 2018

Creation And Use Of Software For Analysis Of Kinetic Proteomic Experiments, Bradley C. Naylor

Theses and Dissertations

In this dissertation, I will review the history and general strategies for performing kinetic proteomics. I will then demonstrate that I have published an open source, user-friendly program for other scientists to use to perform kinetic proteomics data analysis, as well as publishing a novel discovery of key ribosomal subunits being replaced within the lifetime of the ribosome, which was discovered through use of kinetic proteomics. Finally, I will discuss work that is ongoing to improve my software tool for use in human subjects, and work being done to combine kinetic proteomics with other global analysis methods to make novel …

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"Love On The Brain":? Exploring The Influence Of Lyrics On Sexting And Risky Sexual Behavior, Savannah Leigh Kroff Apr 2018

"Love On The Brain":? Exploring The Influence Of Lyrics On Sexting And Risky Sexual Behavior, Savannah Leigh Kroff

Theses and Dissertations

While some research has been done on the influence of music lyrics on sexual behaviors in general, none has explored the lyrical influence on sexting. The goal of the current study is to explore the longitudinal influence of sexual and sexually objectifying lyrics on adolescent sexting behaviors. Participants were 125 14-year-old adolescents who were recruited from high schools in Texas. Participants completed questionnaires on music preferences, sexual behaviors and impulsivity and were given BlackBerry devices through which frequency of sexual text message utterances was obtained. Music preferences were then quantitatively analyzed using the Linguistic Inquiry Word Count (LIWC) software. Logistic …

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A Comparison Study Of Disease Spreading Models, Douglas Nogueira Oliveira Apr 2018

A Comparison Study Of Disease Spreading Models, Douglas Nogueira Oliveira

Theses and Dissertations

Epidemic diseases have become one of the major sources of concern in modern society. Diseases such as HIV and H1N1 have infected millions of people around the world. Many studies have attempted to describe the dynamics of disease spreading. Despite the numerous models, most only analyze one aspect of a given model, such as the impact of the topology of a contagious network, or, the spreading rate of disease. Although useful, these types of analyses do not provide insights into how different models could be combined to better understand the phenomenon, or how the modeling decisions affect the dynamics of …

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Controlled Intake Area Dredging For Fine Sediment Removal To Improve Water Quality In The Indian River Lagoon, Hannah Christine Grisanti Apr 2018

Controlled Intake Area Dredging For Fine Sediment Removal To Improve Water Quality In The Indian River Lagoon, Hannah Christine Grisanti

Theses and Dissertations

This study is an investigation of hydraulic dredging specifically designed to remove muck and other fine sediments from local water systems in Brevard County, Florida. The goal of the project is to improve water quality in the Indian River Lagoon (IRL) and create positive environmental impacts. Dredging is an excavation activity that takes place underwater in shallow seas or freshwater environments to remove material from the bottom surface. Typically, dredging techniques are implemented to keep waterways such as canals, inlets, and harbors navigable for boats and barges. Dredging is also commonly used to move sand from offshore sources to beaches …

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Analysis Of A Functional Heterologous Chromosomal Replication Origin Transplant In E. Coli, Rohit Pradip Kadam Apr 2018

Analysis Of A Functional Heterologous Chromosomal Replication Origin Transplant In E. Coli, Rohit Pradip Kadam

Theses and Dissertations

To ensure that all cells replicate their genomes faithfully before they divide, the initiation step of chromosome replication must be tightly regulated during the cell cycle. In bacteria, new rounds of replication are triggered by the assembly of a higher order protein complex, termed the orisome, that unwinds a well-defined origin (oriC) region on the circular chromosome, and prepares it for new replication forks. Orisomes comprise multiple copies of a highly conserved initiator protein, DnaA, and instructions for ordered orisome assembly are encoded into the oriC nucleotide sequence of nearly all bacteria as specifically-spaced clusters of 9 base pair (bp) …

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Increasing Employee-Guest Interactions Using A Guest-Delivered Token Economy At A Zoological Facility, Cassie Maureen Vergason Apr 2018

Increasing Employee-Guest Interactions Using A Guest-Delivered Token Economy At A Zoological Facility, Cassie Maureen Vergason

Theses and Dissertations

Token economies have been used for many years to improve a multitude of behaviors in a variety of settings. However, research on token economies as an intervention to improve customer service-related behaviors of employees in organizations is limited. The current study assessed this issue by evaluating a guest-delivered token economy to improve employee-guest interactions at a zoological facility. Results showed increases of 35.3% and 45.0% in correct employee-guest interactions in each intervention phase compared to baseline, thus suggesting that a guest-delivered token economy is an effective way to improve customer service (guest interactions)-related behaviors. Limitations of the current study and …

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Inverse And Forward Solutions On Fetal Magnetocardiography: Hybrid Dipole Model, Recep Avci Mar 2018

Inverse And Forward Solutions On Fetal Magnetocardiography: Hybrid Dipole Model, Recep Avci

Theses and Dissertations

In-utero monitoring and assessment of fetal heart are crucial to early diagnose and treat the electro-physiological problems. Fetal magnetocardiography (fMCG) has become an attractive technique for in-utero assessment of cardiac electrophysiology. The measured fMCG signals are affected by the characteristics of the fetal heart and volume conductor which is formed by fetal and maternal tissues, and the position of the fetus with respect to sensors. Therefore, the signal morphology of fMCG shows differences for each measurement which prevents their comparison across subjects. These confounding factors can be removed by applying source localization techniques, which estimate the source parameters of the …

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Negative Outcomes Associated With Childhood Sexual Abuse, Kristen N. Silbert Mar 2018

Negative Outcomes Associated With Childhood Sexual Abuse, Kristen N. Silbert

Theses and Dissertations

The purpose of the study was to examine whether attachment insecurity, global self-esteem, sexual self-esteem, and subjective well-being mediate the relationship between childhood sexual abuse (CSA) and sexual assault during college. The participants included 158 female college students from Illinois State University. Students were granted the opportunity to receive extra credit for participating in the study. Participants completed a demographics questionnaire, the Rosenberg Self-Esteem Scale, the Sexual Self-Esteem Inventory, the Experiences in Close Relationships Questionnaire, the Positive and Negative Affect Schedule, the Satisfaction with Life Scale, the Hot Topics Questionnaire, and the Sexual Experiences Survey. Of the 158 participants, 108 …

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Factors Affecting Illinois Legislators' Perceptions Of School-Based Agricultural Education, Perry William Harlow Mar 2018

Factors Affecting Illinois Legislators' Perceptions Of School-Based Agricultural Education, Perry William Harlow

Theses and Dissertations

This study examined factors contributing to Illinois General Assembly members’ perceptions of school-based agricultural education. Personal characteristics and multiple media sources were examined to determine if any of these factors affected their perception of school-based agricultural education. These factors included the population area of where the legislator grew up, the population area of the district the legislator represents, the geographical location of the legislator’s district, whether the legislator lived on a farm, and whether the legislator worked on a farm. The factors also included frequency of seeing information about school-based agricultural education on multiple media source including television, radio, newspaper, …

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A Study Of Collaboration Between The Defense Logistics Agency And The U.S. Agency For International Development In The Conduct Of Humanitarian Operations, Jessica M. Thomas Mar 2018

A Study Of Collaboration Between The Defense Logistics Agency And The U.S. Agency For International Development In The Conduct Of Humanitarian Operations, Jessica M. Thomas

Theses and Dissertations

Many organizations are finding it advantageous and often necessary to form collaborative alliances with strategic partners in order to solve collective problems and jointly work towards mutually desirable ends. This research examines a single case study of inter-agency coordination between the Defense Logistics Agency (DLA) and the U.S. Agency for International Development (USAID) and their involvement in humanitarian relief efforts. Pursuit of advancement through two collaboration models provided the framework of the research and contributed to the analysis of data. Resulting outcomes offer incentives for both organizations to develop stronger social networks assisting in a deeper understanding of the others …

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Characterization Of The Effects Of Heavy Charged Particle Exposure On The Radiation Resistant Bacterium Deinococcus Radiodurans, Todd A. Bryant Mar 2018

Characterization Of The Effects Of Heavy Charged Particle Exposure On The Radiation Resistant Bacterium Deinococcus Radiodurans, Todd A. Bryant

Theses and Dissertations

Although not an extremophile, Deinococcus radiodurans has proven to have exceptional resistance to ionizing radiation, specifically via gamma and X-ray photons. To date, no known experiments have bombarded the bacterium with charged particles larger than hydrogen. This study explored the effects on the organism’s ability to survive high linear energy transfer heavy-charged particle exposures of oxygen ions. It also investigated the effects of low linear energy transfer ultraviolet radiation on various mutants. Two uvrB mutants were created to ascertain the importance of the gene in single-strand break repair following gamma irradiation and two recF mutants were created to explore the …

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Characterization Of Silicon Ion Exposure On Deinococcus Radiodurans, Richard F. Daughtry Mar 2018

Characterization Of Silicon Ion Exposure On Deinococcus Radiodurans, Richard F. Daughtry

Theses and Dissertations

Deinococcus radiodurans is a robust bacterium that is known for its extraordinary resistance to ionizing radiation. In general, many of the investigations of this bacterium's resistance have revolved around low linear energy transfer radiation, such as gamma and electron radiation. This study explored Deinococcus radiodurans's ability to survive high linear energy transfer radiation, specifically proton and neutron radiation. Deinococcus radiodurans was dehydrated to reduce the effects of low linear energy transfer radiation. The bacteria were exposed to both neutron and proton radiation of varying amounts and rehydrated. The resulting colonies were counted and compared to colonies of non-irradiated control samples …

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Cognitive Effects Of Short Duration Short Wavelength Visible Light, Sarah J. Bolton Mar 2018

Cognitive Effects Of Short Duration Short Wavelength Visible Light, Sarah J. Bolton

Theses and Dissertations

This thesis research looks at the effects of short duration, high intensity, short wavelength light with the goal of determining if converting typically white colored lights in breakrooms and bathrooms to blue will cause workers who normally work in a low light environment to be more alert and productive. Sixteen participants were outfitted with Electroencephalography (and Electrooculography (EOG) equipment before being exposed to 200 lux of either 460 nm blue light or D6500 white light for 30 minutes. Afterwards, the light levels were changed to 3.5 lux of D6500 light and the participants were asked to perform six cognitive tests …

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Estimating Defensive Cyber Operator Decision Confidence, Markus M. Borneman Mar 2018

Estimating Defensive Cyber Operator Decision Confidence, Markus M. Borneman

Theses and Dissertations

As technology continues to advance the domain of cyber defense, signature and heuristic detection mechanisms continue to require human operators to make judgements about the correctness of machine decisions. Human cyber defense operators rely on their experience, expertise, and understanding of network security, when conducting cyber-based investigations, in order to detect and respond to cyber alerts. Ever growing quantities of cyber alerts and network traffic, coupled with systemic manpower issues, mean no one has the time to review or change decisions made by operators. Since these cyber alert decisions ultimately do not get reviewed again, an inaccurate decision could cause …

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United States Air Force Applications Of Unmanned Aerial Systems: Modernizing Airfield Damage Assessment, Samuel M. Clark Mar 2018

United States Air Force Applications Of Unmanned Aerial Systems: Modernizing Airfield Damage Assessment, Samuel M. Clark

Theses and Dissertations

Modernizing airfield damage assessment has long been a priority mission at the Air Force Civil Engineer Center (AFCEC). Previously, AFCEC has made advances to expedite unexploded ordnance (UXO) neutralization and pavement repair. Missing from these initiatives is the initial assessment component. This thesis expands the idea of using Small Unmanned Aerial Systems (SUAS), applies it to the Air Force mission, and provides SUAS vehicle configuration and sensor recommendations. In this study, 25 civil engineer officers reviewed airfield imagery gathered using two small air vehicles. For the first review, participants attempted to identify UXOs and foreign object debris (FOD) in a …

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Stabilized Rpa Flight In Building Proximity Operations, Michael M. Kaniut Mar 2018

Stabilized Rpa Flight In Building Proximity Operations, Michael M. Kaniut

Theses and Dissertations

The thesis seeks a solution to the requirement for a highly reliable and capable Unmanned Air Vehicle (UAV) to support a wide array of missions and applications that require close proximity flight to structures. The scope of the project includes the drafting of a concept of operations (CONOPs) describing how the mission requirements might be met using the sensor, operators, and air vehicle described here in. The demonstration of the wall-following section of that CONOPs is performed by cart testing a custom algorithm and evaluating its ability to react to its environment. Finally, a flight test was performed to characterize …

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Cyber Data Anomaly Detection Using Autoencoder Neural Networks, Spencer A. Butt Mar 2018

Cyber Data Anomaly Detection Using Autoencoder Neural Networks, Spencer A. Butt

Theses and Dissertations

The Department of Defense requires a secure presence in the cyber domain to successfully execute its stated mission of deterring war and protecting the security of the United States. With potentially millions of logged network events occurring on defended networks daily, a limited staff of cyber analysts require the capability to identify novel network actions for security adjudication. The detection methodology proposed uses an autoencoder neural network optimized via design of experiments for the identification of anomalous network events. Once trained, each logged network event is analyzed by the neural network and assigned an outlier score. The network events with …

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A Surprising Symbiosis: Examining The Mutualism In Department Of Defense Conservation Partnerships, Andrew J. Lankow Mar 2018

A Surprising Symbiosis: Examining The Mutualism In Department Of Defense Conservation Partnerships, Andrew J. Lankow

Theses and Dissertations

Because of the DoD’s unique and significant role in the conservation of natural resources, military leaders and natural resource managers need a trail guide to frame interagency collaboration in a way that shapes productive partnerships. The purpose of this research was to examine successful DoD conservation partnerships in order to identify key success factors (KSFs) and the most prominent challenges faced, and how those characteristics enable the accomplishment of each partner’s objectives. The research questions were answered through a comprehensive literature review and the use of the multiple-case study method. 19 key informants from three installations participated in semi-structured interviews, …

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Parametric Survival Analysis Of Us Air Force Rated Officer Retention, Jacob R. Lindell Mar 2018

Parametric Survival Analysis Of Us Air Force Rated Officer Retention, Jacob R. Lindell

Theses and Dissertations

Personnel retention is a very important topic in both the private and public sectors. Not only do companies need to make sure they have the right people, they need to have the right amount of people. Within the public sector, specifically the US Air Force, maintaining appropriate manning comes in two phases; bringing in the right amount of people each year, and retaining enough people from year to year. These two aspects go hand in hand; if the Air Force knows how many people they will lose in a given year, they can bring in the exact number of people …

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The Application Of Text Mining And Data Visualization Techniques To Textual Corpus Exploration, Jeffrey R. Smith Jr. Mar 2018

The Application Of Text Mining And Data Visualization Techniques To Textual Corpus Exploration, Jeffrey R. Smith Jr.

Theses and Dissertations

Unstructured data in the digital universe is growing rapidly and shows no evidence of slowing anytime soon. With the acceleration of growth in digital data being generated and stored on the World Wide Web, the prospect of information overload is much more prevalent now than it has been in the past. As a preemptive analytic measure, organizations across many industries have begun implementing text mining techniques to analyze such large sources of unstructured data. Utilizing various text mining techniques such as n -gram analysis, document and term frequency analysis, correlation analysis, and topic modeling methodologies, this research seeks to develop …

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Evaluating The Air Force Inspection System, Luis D. Rosado-Medina Mar 2018

Evaluating The Air Force Inspection System, Luis D. Rosado-Medina

Theses and Dissertations

SAF/IG implemented the Air Force Inspection System (AFIS) in 2013 following a USAFE pilot study into its potential for implementation across the Air Force. Using responsive constructive evaluation and content analysis of interviews as a methodology, AFIS was evaluated against the objectives laid out in AFPAD13-01. 18 interviews were conducted on 4 stakeholder groups across the inspection enterprise, which resulted in 4 overarching themes: Culture Shifts, Self-Assessment, Higher Headquarters Relationships, and PAD Objectives. This research allows SAF/IG to implement changes to future versions of AFIS that incorporates views from the field.

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Assessment Of Structure From Motion For Reconnaissance Augmentation And Bandwidth Usage Reduction, Jonathan B. Roeber Mar 2018

Assessment Of Structure From Motion For Reconnaissance Augmentation And Bandwidth Usage Reduction, Jonathan B. Roeber

Theses and Dissertations

Modern militaries rely upon remote image sensors for real-time intelligence. A typical remote system consists of an unmanned aerial vehicle, or UAV, with an attached camera. A video stream is sent from the UAV, through a bandwidth-constrained satellite connection, to an intelligence processing unit. In this research, an upgrade to this method of collection is proposed. A set of synthetic images of a scene captured by a UAV in a virtual environment is sent to a pipeline of computer vision algorithms, collectively known as Structure from Motion. The output of Structure from Motion, a three-dimensional model, is then assessed in …

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Land Cover Influences On Airborne Noise Propagation At Usaf Installations, Ariel L. Waiters Mar 2018

Land Cover Influences On Airborne Noise Propagation At Usaf Installations, Ariel L. Waiters

Theses and Dissertations

Mitigation of aircraft noise and encroachment by residential areas at various U.S. military installations remains a challenge and hampers effective military operations. The Air Force has established standard practices that utilize computational noise models to generate and display noise propagation from military installations. Despite great advances in models and mitigation action plans, the current USAF Noisemap modeling tool and methods do not consider the effects of variation in land cover. Moreover, such limitations could greatly affect the accuracy of predicting aircraft noise pollution. The standard modeling method assumes a ground cover impedance of 225 Kpa*s/m2, which …

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