Digital Commons Network^™

Augmenting Guadecitabine To Promote Anticancer Immunity In Murine Breast Cancer, Timothy M. Smith Jr.

Theses and Dissertations

In immune-competent individuals, tumor growth occurs due to a failure of the immune system to recognize and destroy malignant cells. Immune surveillance can be curtailed by the presence of immunosuppressive cells such as myeloid-derived suppressor cells (MDSCs) and T-regulatory cells (Tregs) which both serve to dampen antitumor immunity. These studies utilize a metronomic low-dose therapeutic approach to examine the effects of anticancer drugs against murine breast cancer and the effects on the immune cell compartments. The focus of these studies revolve around the drug guadecitabine (guad), a second-generation DNA methyl-transferase inhibitor (DNMTi). DNMTi’s have been shown to dysregulate the methylation …

Acute Stress Disorder In Neonatal Intensiver Care Unit Mothers: Modeling Risk Factors And Trauma Appraisals Modeling, Allison Baylor 5435882

Theses and Dissertations

Postpartum mental health is a public health priority, particularly for mothers with infants in the Neonatal Intensive Care Unit (NICU), who may have experienced a precipitous birth, followed by a traumatic NICU hospitalization. However, little research has focused on NICUrelated posttraumatic stress (PTSS), despite its presumed prevalence and ripple effects on infant development, family functioning, and provider burnout during the critical early bonding period. In particular, little research has evaluated: early posttraumatic stress (Acute Stress Disorder symptoms, ASD), index and sequential traumas related to NICU/birth, and relations among preexisting risk factors, and trauma appraisals/ objective trauma characteristics (related to infant …

Synthesis Of Multifunctional Nanoparticles For Imaging And Enhancement In Therapy, Gabrielle Seymore

Theses and Dissertations

The purpose of this work was to synthesize nanoparticles composed of high atomic number elements and semiconductor material in a core/shell structure for the potential to be used as enhancers for radiotherapy as well as luminescence imaging platforms. Additionally, to quantify their role in free radical production after exposure to ionizing radiation through chemical routes. Spherical gold nanoparticles were synthesized via a citrate stabilizer method. Two sizes of 12nm and 25 nm gold spheres were used as the cores for the europium-doped gadolinium vanadate flower-shaped shell. The production of 7-hydroxycoumarin-3-carboxylic acid in an aqueous environment upon kV irradiation of its …

The Role Of Ca2+/Calmodulin Dependent Protein Kinase Ii, Camk-Ii, In Kidney Morphogenesis, Sarah R. Ingram

Theses and Dissertations

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common heritable diseases in the world, characterized by the development of large fluid filled cyst. Treatments for this disease are limited due to a lack of understanding of disease pathogenesis. Ca²⁺/calmodulin dependent protein kinase II (CaMK-II) is necessary for kidney morphogenesis in zebrafish as well as being a downstream effector of pkd2, one of the genes most commonly mutated in ADPKD patients. The roles of CaMK-II during zebrafish kidney development include regulation of kidney cell migration as well as cloacal cilia stability. The influence of CaMK-II …

Sleep Behaviors And Thoughts As Links Between Social Rhythmicity And Mental Health Outcomes, Sahar Sabet

Theses and Dissertations

Social rhythmicity refers to the regularity with which one engages in social and lifestyle activities. Considerable research has recognized that social rhythms have important implications for health and well-being. For example, disturbances in social rhythms are one potential factor that may contribute to the development of anxiety and depressive disorders. Existing research on social rhythm irregularity has heavily focused on its link with bipolar disorder and, to a lesser extent, with depression. Less is known about the association of social rhythms with anxiety or with subclinical levels of depression. Furthermore, it is unclear how social rhythms are tied to mental …

Validation Of Ninein As An Ethanol-Related Quantitative Trait Gene: Reassessment, Design, And Functional Validation Of Reference Genes For Qpcr Analysis Of Brain Tissue In Mice, Jessica L. Jurmain

Theses and Dissertations

The increasing use of quantitative real-time polymerase chain reaction (qPCR) as a method for quantifying gene expression has led to an increased demand for standardization of data analysis methods to ensure accurate reporting and robust, reproducible results. The exponential nature of qPCR amplification results in the potential magnification of what are usually very small sources of error. Relative gene expression calculations circumvent this issue by normalizing target gene expression data to within-sample expression of a previously validated, stably expressed reference gene or genes. Multiple studies discussed herein have found that qPCR data are more reliable and reproducible when multiple reference …

The Functional Importance Of Methyl Cpg Binding Domain Proteins 2 And 3 In Regulating Fetal Hemoglobin Expression In Human Adult Erythroid Cells, Alexander Azzo

Theses and Dissertations

Humans undergo two developmental switches in the predominantly expressed β-like globin chain during embryogenesis and fetal development. The first switch from embryonic (ε) to fetal (γ) occurs around week 5 of embryonic development, while the second switch from fetal to adult (β) globin occurs shortly after birth. By adulthood, fetal hemoglobin represents only 1-2% of total hemoglobin in the blood. As sufficiently elevated levels of fetal hemoglobin are beneficial for improving clinical outcomes in sickle cell disease and β-thalassemia, the mechanisms that enforce silencing of fetal hemoglobin expression postnatally are of great clinical significance. The methyl-CpG binding domain protein MBD2 …

A Study On The Mechanism Of Clp290 Restoration Of Kcc2 And Neuroprotection After Traumatic Brain Injury, Ekaterina Stepanova

Theses and Dissertations

The research of this thesis investigated the mechanism of action for the therapeutic compound CLP290, which our laboratory has previously demonstrated to reverse degradation of the protein potassium-chloride co-transporter 2 (KCC2) and restore neuronal function following modeled traumatic brain injury (TBI) in the rat. The loss of membranous KCC2 from the plasma membrane after TBI worsens the excitation/inhibition imbalance common in neurotrauma. Addressed here were the specific aims to: 1) assess that CLP290 treatment restores the functional oligomeric form of KCC2 to the neuronal plasma membrane as necessary to provide chloride homeostasis; 2) investigate the signaling mechanism by which CLP290 …

Disruption Of The Dream Complex Results In Cell Cycle Deregulation, Hayley C. Walston

Theses and Dissertations

The Dimerization Partner, Rb-like, E2F, And MuvB (DREAM) complex was identified in humans due to homology of the genes involved in the D. melanogaster dREAM complex. It assembles during cellular arrest, or G0, to function as a transcriptional repressor of cell cycle genes. It is known that phosphorylation of LIN52 at serine 28 is necessary for the critical DREAM forming interaction between LIN52 and the pocket protein p130. Our laboratory has previously shown that gene editing of LIN52 to replace serine 28 with alanine (S28A) prevents phosphorylation by the kinase DYRK1A and inhibits DREAM formation. Here we have confirmed this …

Regional Drivers Of Organic Carbon Age In Lotic Systems Of The Conterminous United States, Kaycee Faunce

Theses and Dissertations

Rivers play a critical role in global carbon (C) budgets despite their comparatively small surface area. A significant portion of the terrestrial C that they receive is transformed, re-mineralized, or stored during transit to the ocean. Radiocarbon (∆¹⁴C) data show that a fraction of riverine organic C (OC) has been pre-aged in the terrestrial environment. Lateral export of carbon from these aged pools may contribute to atmospheric carbon dioxide emissions through microbial and photochemical oxidation. However, little is known about the regional climatic, anthropogenic, and landscape factors that promote the mobilization of aged OC to rivers. This study …

Non-Invasive Retrieval Of Prefabricated Zirconia Crowns With Er,Cr:Ysgg Laser From Primary And Permanent Teeth, Connor W. Mccall Dds

Theses and Dissertations

Purpose: Compromised tooth structure on permanent and deciduous teeth in the pediatric population is increasingly being restored with tooth-colored prefabricated zirconia crowns. These restorations may need to be removed or replaced with permanent crowns. The purpose of this in vitro study was to explore the use of an Er,Cr:YSGG laser for removal of prefabricated zirconia crowns cemented with RMGI as a non-invasive alternative to rotary instruments.

Methods: Thirteen permanent and 12 primary molars were prepared to dentin and prefabricated zirconia crowns were passively fitted and cemented with two resin modified glass ionomer cements. The irradiation parameters for ErCR:YSGG were 4.5 …

Selenomonas Sputigena: Growth Conditions And Projected Virulence Factors Of An Emerging Oral Pathogen, Kayla A. Keller

Theses and Dissertations

Periodontal diseases are ubiquitous diseases. They can affect people through a chronic infection that happens over a lifetime, but also as an aggressive infection that afflicts the younger population. It not only results in tooth loss and a lower quality of life, but it can also lead to fatal secondary infections like cervical cancer, heart disease, and Alzheimer’s disease. This study looks at Selenomonas sputigena, an emerging oral pathogen, that has been hypothesized to contribute to periodontal disease. It has been linked to fatal septicemia and has been found in increased abundance within the oral biofilm during peak infection. …

Enhancing Chemosensitivity Of Lung Cancers By Manipulating The Tumor Microenvironment: Local Lung Delivery And Nanocarrier-Based Therapies, Hanming Zhang

Theses and Dissertations

Cancer is the second leading cause of death in United States causing an estimated six hundred thousand death in 2019. Among different types of cancer, lung cancer is the leading cause of cancer related death in both male and female. While targeted therapy and immunotherapy have shown promising clinical outcomes, currently there remain a significant proportion of patients who do not benefit from these strategies or who cannot tolerate them, making Pt based doublet chemotherapy still an indispensable component in almost all stages of treatment. However, partially due to the poor tumor distribution of small molecule drug, the development of …

Impact Of Student’S Gender And Perceived Skin Tone On Educators’ Disciplinary Decisions, Kierstyn K. Johnson-Wigfall

Theses and Dissertations

The purpose of this proposed dissertation is to examine the relationship of students’ skin color and gender to school disciplinary decisions for African American children. While skin tone bias or colorism is frequently studied, limited research has been completed about its prevalence in American public schools during the discipline process. For example, school suspension leads to higher rates of absenteeism, lower academic achievement and higher chances of incarceration. Even students who receive an office referral and return to c lass minutes later miss pertinent instruction time. This study aims to: 1) add to the body of literature about this topic, …

Work Hope And Work Volition: Exploring The Influence Of Community College Students’ Rurality And Socioeconomic Status, Jesse A. Wingate

Theses and Dissertations

Data from a sample of community college students (N = 478) in Virginia were used to examine relations among rurality, socioeconomic status, work hope, and work volition. Socioeconomic status, work hope, and work volition were positively associated. However, rurality, measured both as a continuous and categorical variable, was not correlated with work hope or work volition. Additional analyses showed convergence between measures of work hope and work volition confirming construct similarity. Results, limitations, implications, and recommendations for future study are included.

Preventative And Therapeutic Effects Of Bmsc Exosome On Periodontal Disease, Jin Ha Kim

Theses and Dissertations

Periodontitis is an inflammatory disease caused by the imbalance between host immune response and bacterial infection. Strategies to manage the uncontrolled, excessive immune response and to promote tissue healing are in great demand. Therapies based on mesenchymal stem cells (MSCs) are promising and the clinical effects of MSCs are largely mediated by their secretome, especially exosomes. Previously, we isolated and purified exosomes secreted by human bone marrow mesenchymal stem cells (hBMSC). These exosomes have proliferative, chemotactic, anti-inflammatory and anti-osteoclastic function in vitro. In this study, we had two hypotheses: 1) hBMSC exosomes would prevent periodontal inflammation and alveolar bone loss …

Physical Activity And Depression In Mothers Of A Child With A Special Health Care Need: Informing Future Interventions, Brianna Jean Black

Theses and Dissertations

In the United States, approximately 20 percent of all children have a special health care need. Additionally, mothers of a child with a special health care need have worse overall well-being, mental and physical health compared to mothers of typically developing children. Physical activity may improve depressive symptoms and overall health in these mothers; however, little is known about the acceptability of physical activity interventions for mothers of a child with a special health care need. Therefore, the primary aim of this study was to understand more about mother’s desire to use physical activity to improve depressive symptoms after having …

A Study Of The Feasibility Of Growth, Extraction, And Industrial Scale Up Processing Of Microalgae Lipids., Jasmine Oleksik

Theses and Dissertations

The interest in microalgae as a plausible alternative to crop oils as a raw material in the form of triglycerides and free fatty acids (FA oils) for renewable fuels and chemicals is increasing and it is a widespread research topic at the lab scale. Microalgae contain a higher lipid content on a dry weight basis compared to oilseeds such as soybeans. Additionally, the growth and cultivation cycle of microalgae is 15 days, in comparison to soybeans where the cycle occurs once or twice annually. Despite these advantages, to date it has been uneconomical to produce microalgae oils in a world-scale …

Bio-Regenerative Life Support Systems Functional Stability And Limitations, A Theoretical Modeling Approach, Curt I. Holmer

Theses and Dissertations

Experiments with Ecological Closed Life Support Systems (ECLSS) for moderate sized crews have shown instability when supporting crews over long periods of time required for deep space travel. Tests such as Russia’s BIOS series, NASA’s Lunar-Mars Life Support Test Project (LMLSTP), ESA’s Micro-Ecological Life Support System Alternative (MELiSSA), and Japan’s Closed Ecology Experiment Facilities (CEEF) have shown that microalgae and higher plants combined with physical-chemical material converters can be a successful part of a Biological Life Support System or a Closed Ecological Life Support System. LMLSTP, MELiSSA, and CEBAS experiments as well as commercial Ecosphere products have proven stability at …

What Role Can Social Cognitive Neuroscience Play In Promoting Prosocial Behaviors In Organizations, Lynne Forbes-Zeller

Theses and Dissertations

The purpose of this research is to establish a correlation between the integration of Social Cognitive Neuroscience (SCN) frameworks (e.g., The SCARF model) into day-to-day social interactions in the workplace. The study also sought to determine whether SCN practices can increase prosocial behaviors and improve a leader's influence. This qualitative study had four phases: an educational video, a self-assessment, a learning workshop, and an experiential learning exercise. Data were collected through eight interviews. This study revealed that understanding the brain's influence in social environments increased prosocial behaviors and positively impacted leader thoughts and actions. Data showed taking the self-assessment, learning …

Best Leadership Practices Of Female Film Directors, Sara Carraway

Theses and Dissertations

Female film directors are highly underrepresented within the U.S. film industry, especially within narrative film (S.L. Smith, Pieper, & Choueiti, 2013). In 2019, only 12% of directors in the top 100 grossing films were female (Lauzen, 2020). There are several obstacles female directors face in their careers. Financing is more difficult to obtain for female directors due to stereotypes of women as risky investments (P. Smith et al., 2013). Closely tied to financing, gendered networks and homophily can prevent women from making relationships with gatekeepers and accessing the same opportunities as men (Jones & Pringle, 2015; Wing-Fai et al., 2015). …

Science And Spirituality As Applied To Od: The Unique Christian Science Perspective: A Qualitative Research Study, Charlotte Booth

Theses and Dissertations

This qualitative study researched Christian Science principles, a blended approach of science and spirituality, and their relevance and contribution to the field of Organization Development. Data was gathered as to whether or not Christian Science principles should be considered a resource for handling business challenges. The 14 individuals who participated in this study were asked 15 questions that correlated to the primary three research questions. Participant's answers were collected and coded in order to analyze and determine reoccurring and prominent points in the data. The study found that some current OD methods and tools are similar in context to Christian …

A Phenomenological Study Of Flourishing In Mid-Career Professionals, Sohyun Lee

Theses and Dissertations

Prior research shows that mid-career professionals experience more negative emotions such as regret, confusion, disappointment, frustration and anger than positive feelings. Yet, many mid-career professionals are flourishing in their careers and not experiencing such negativity. The purpose of this qualitative, phenomenological study was to explore the lived experiences of five professionals who self-identify as flourishing in mid-career. This study also addressed the literature gap concerning mid-career professionals who are flourishing. Prior studies did not utilize the up-to-date model of flourishing and specifically, Seligman's well-being theory and its PERMA-H (positive emotions, engagement, positive relationships, meaning, achievement, and positive physical health) model, …

How Does Eye Movement Desensitization And Reprocessing (Emdr) Work? An Examination Of The Potential Mechanisms Of Action, Sara Forster

Theses and Dissertations

A mounting body of evidence suggests that Eye Movement Desensitization and Reprocessing therapy (EMDR) is successful in reducing the impact of posttraumatic symptoms. Although the exact mechanisms of action remain unknown, theories from the psychological to the neuroscientific continue to emerge, expand, and evolve. This study will examine four of the most prominent theories to date and weigh the evidence for and against each one. It will also review, compare, and contrast the theories, evaluate the research supporting each one, and propose the most likely explanation for EMDR's success given the state of the research. Neurobiological mechanisms and correlates as …

Understanding Gender Roles In The Workplace: A Qualitative Research Study, Andrea Michel

Theses and Dissertations

This qualitative study explored female leaders' experiences with gender norms, implicit bias and microaggressions that they have experienced over the course of their careers. Research questions explored what gender norms exist, how they show up behaviorally in the workplace, and how gender norms, implicit bias and microaggressions impact women. 12 women participated in the study and were asked 12 interview questions. Participants' answers to these questions were coded to highlight themes. Themes were identified as communication diplomacy, leadership style, family commitments, physical appearance, and pink roles for data analysis. Key themes were summarized, and key research findings were discussed. Key …

Mapping Land Use And Land Cover Change And Its Impact On Landscape Structure And Amphibian Functional Connectivity In The Prairie Pothole Region, Taylor Holm

Theses and Dissertations

Monitoring land use and land cover (LULC) change and its effect on habitat availability and connectivity is crucial for species conservation and management. This is most efficiently accomplished at a sufficiently broad scale using remote sensing technology. Current approaches benefit from freely available data sources such as National Land Cover Database (NLCD), derived from 30 m Landsat imagery. However, temporal frequency and spatial resolution of NLCD may be insufficient for some applications. Specifically, 30 m resolution data may not detect small or narrow landscape features that may be important habitat for small animals such as amphibians. Other available data sources, …

Mountain Lions In North Dakota: A Public Opinion Survey, Theodore A. Darnell

Theses and Dissertations

Breeding mountain lion (Puma concolor) populations currently inhabit the western portion of North Dakota. In recent years, apparent increases in human-lion interactions across North Dakota, and other Midwest states, have challenged wildlife managers who strive to balance biological and social carrying capacities of these animals. In 2019, we surveyed 2,000 residents of North Dakota, USA, using a self-administered mail survey to discern knowledge of and attitudes towards mountain lions, and to explore whether the presence of these animals would influence decisions to recreate in western North Dakota. Our questionnaire included 12 questions designed to ascertain respondent attitudes towards mountain lions. …

Montana Coaches' Perceptions On Including Transgender Athletes In High School Interscholastic Sports And Its Effect On Team Chemistry, David L. Woods Ii

Theses and Dissertations

The eligibility of transgender students to participate in high school athletic sports is a polarizing topic in the United States of America. Questions of fairness within each competitive sport, along with precedents set in law and policy, both by the United States and by International Sporting Organizations such as the International Olympic Committee, shape arguments for or against transgender high school students' eligibility. Each state faces the decision of whether to base high school transgender athletes' eligibility in high school sports according to their gender identity, or their sex assigned at birth.

The study aimed to identify Montana high school …

Synthesis And Design Of Novel Polymer Grafted Nanoparticles Relevant To Drug Delivery Vehicles For Biomedical Application, Maan Abduldiyem Hassan Al-Ali

Theses and Dissertations

The modification of inorganic nanoparticles with organic polymer chains has become a significant field of study for the engineering of advanced nanocomposite materials. This dissertation presents the design, synthesis, and characterization of novel polymer grafted silica nanoparticles as new strategies to combat bacterial resistance. Described herein is the synthesis of monomers that have been graft polymerized onto silica nanoparticles that can be used as a delivery drug vehicle for biomedical applications. The polymerization of these monomers was performed via reversible addition-fragmentation chain transfer (RAFT) polymerization. The molecular design of the RAFT agents that are attached to the surfaces of the …

Epigenetic Alterations At Synaptic Plasticity Genes In A Genetically Heterogeneous Rat Model Of Neuropsychiatric Disorders, Doan M. On

Theses and Dissertations

Sensorimotor gating impairments are observed across a range of neuropsychiatric conditions. The prepulse inhibition of the acoustic startle response (PPI) is a validated measure of sensorimotor gating. Genetic and pharmacological manipulations in rodents have shown PPI is regulated by specific brain monoaminergic systems. Using genetically heterogeneous NIH-HS rats, we stratified individuals by %PPI. In low PPI animals, we observed elevated mRNA levels of certain neurotransmitter receptors, including metabotropic glutamate receptor Grm2, dopamine receptors Drd1 and Drd2, serotonin receptors Htr1a and Htr2a, and scaffolding protein Homer1, in the frontal cortex (FC) and striatum (STR). We found Drd2 …