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Characterizing The Transport Of Parasitoid Wasp Genaspis Hookeri Venom Serca Into Host Drosophila Hemocytes, Christopher Lark

Theses and Dissertations

The female endoparasitoid wasps introduce venom accompanied by an egg into the larval hosts’ body cavity during oviposition. The venom contains a mixture of proteins that exploit the host’s biological processes, such as, regulating gene expression, disrupting immune signaling pathways, or inducing immune cell death to give a decided advantage to the incipient wasp to develop unchallenged. Drosophila possesses a highly conserved innate immune response that recognizes and neutralizes foreign microbes and macroparasites. The venom of Ganaspis hookeri (strain G1) endoparasitoid wasp has co-evolved to suppresses the humoral phase Drosophila’s innate immune response by inhibiting Ca2+ bursts needed to induce …

Treatment Of Bakken Produced Water Using Supercritical Water Desalination, Joshua Oloruntimilehin Oluwayomi

Theses and Dissertations

Recent advances in horizontal drilling and hydraulic fracturing have significantly increased freshwater usage. Oil and gas wells in the United States tend to generate over 23 billion barrels of produced water per year of which about 32 million b/day are high total dissolved solids (TDS > 200,000 mg/L) while the world is gradually running short of fresh water. Environmental concerns associated with water usage and concentrated wastewater disposal have led to the growing consideration to treat and recycle brine for reusable purposes like crop irrigation, livestock watering and hydraulic fracturing.

In this study, an emerging and promising technology called supercritical water …

Land Management And Plant Species Pattern Effects On Grassland Pollinator Services, Leslie Blaine Yellow Hammer

Theses and Dissertations

Invertebrate pollinator populations are widely recognized as declining because of anthropogenic activities that include changes in the amount and quality of available habitat, competition with introduced species, increased agricultural chemical use, and climate change. Because habitat and resource availability affect pollinator populations, land management may affect pollinator presence. My first aim was to determine if pollinator communities and habitat characteristics (available bare ground and floral resources) differed between grazed and idle grasslands within the Grand Forks County Grasslands of northeastern North Dakota, USA. Although available bare ground and plant species richness differed between grassland types, floral resources and pollinator communities …

Evaluating Mixed-Mode Survey Designs For Collecting Deer And Fall Turkey Hunter Harvest Data In North Dakota, Ethan James Kalinowski

Theses and Dissertations

Achieving state wildlife agency biological goals for deer (Odocoileus spp.) and turkey (Meleagris gallopavo) rely on high quality data collection via hunter harvest surveys. Concomitantly, better information is needed to optimize best survey methods. From 2017 to 2019, we surveyed North Dakota, USA, deer and fall turkey hunters using a self-administered mail control survey and 3 mixed-mode internet/mail surveys to gain a better understanding of alternative survey designs that may be used to estimate harvest of game populations and inform future management efforts. Our first objective was to measure response rates across various segments of deer and fall turkey hunters …

Interferon-Γ Medaites Lung Damage Through The Hyper-Activation Of Ccr2+ Inflammatory Monocytes During Influenza Virus Infection, Mitchell Anthony Klomp

Theses and Dissertations

Influenza viruses (IV) have caused untold morbidity and mortality throughout the previous centuries. These viruses have caused several pandemics that have witnessed the loss of millions of lives. Today, IVs continue to circulate annually, and the efficacy of influenza virus vaccines are limited. As IV pandemics in 1918, 1957, and 2009 have shown the world, the push for a universal influenza vaccine has never been more relevant. While much research has been devoted to understanding the virology around IVs, the immunopathology of the host-pathogen axis has been less studied. Recently, there has been much attention on the role of the …

Molecular Simulation Study Of Enhanced Oil Recovery Methods In Tight Formation, Chuncheng Li

Theses and Dissertations

The Middle Bakken Formation of the Williston Basin is a typical tight formation with the predicted primary oil recovery of less than 10%, which results in large amounts of oil remaining in the reservoir. Therefore, an effective enhanced oil recovery (EOR) method for recovering the residual oil is crucially important. To obtain the microscopic EOR mechanisms, molecular simulation methods including Monte Carlo (MC) simulations and molecular dynamics (MD) simulations were applied to study the various EOR methods, such as CO2 injection, hydrocarbon gas injection, and nanofluid flooding. A series of molecular models, including bulk systems, interfacial systems, and nanoconfined systems, …

Critical Contingency Eva’S For 3d-Printed Planetary Habitats, James Robert Stoffel

Theses and Dissertations

This novel pilot study evaluates the potential associated tools, methods, and concept of operations required for a Critical Contingency Extravehicular Activity (CCE) repair of a 3D-printed in-situ resource utilization (ISRU) Lunar and Martian habitat. With NASA’s current 2020 Technology Roadmap and Taxonomy for TX06: Human Health, Life Support, and Habitation Systems (e.g. Extravehicular Activities, Habitation, Human Factors), TX07: Exploration Destination Systems (e.g. ISRU, Maintenance, Repair, Operations, Safety), TX12: Materials, Structures, Mechanical Systems, and Manufacturing, and the Centennial 3D-Printed Habitat Challenge, this research will provide an initial evaluation of standard repair methods on the system’s functional abilities, environmental, and human factors …

Asexual Experiences Of Microaggressions Scale: Instrument Development And Evaluation, Evelyn Estela Ayala

Theses and Dissertations

The present paper explores the experience of microaggressions among asexuals and relevant literature. There is a dearth of research on asexuality, particularly as it relates to the experience of microaggressions. However, research suggests that microaggressions have a cumulative impact on both physical and emotional well-being as reported by all minority groups that face microaggressions (Lewis, 2009, Mayer, 2010). The development of a scale that measures the experience of microaggressions would allow for this area of research to be further studied. The paper utilized DeVellis’s (2017) method for scale development to develop a psychometrically sound scale assessing the experience of microaggressions …

An Exploration Of Indigenous Spiritual Microaggressions, Cerynn Dawn Desjarlais

Theses and Dissertations

This descriptive exploratory research is amongst the first to investigate Spiritual Microaggressions experienced by Indigenous Peoples across North America. Microaggressions can further be conceptualized and categorized as microassaults, microinsults, and microinvalidations. Participants who self-identify as Indigenous individuals ages 18 and over, from North America, who work in or are educated in the mental health field engaged in 60-90-minute interviews to explore the spiritual microaggressions incurred upon them. A total of 8 participants who identify as mental health professionals and graduate students in the mental health field were interviewed as an intensity sample. Participants discussed the implications of Indigenous Spiritual Microaggressions …

At-Home Childcare Providers’ Perceptions Of Gardening With Children, Erin Lauckner

Theses and Dissertations

Children do not consume recommended amounts of fruits and vegetables, which has been correlated with negative health consequences. Gardening with children has been shown to positively impact children’s consumption of these foods. While research investigating gardening with children exists, much of this has been conducted with teachers and children in schools, and limited research has been conducted with childcare providers and preschool-aged children. Little research has been conducted about this topic with in-home childcare providers and the children under their care. This pilot study utilized a cross-sectional mixed methods research design through an online survey instrument. A convenience sample of …

Electron Beam Irradiation As A Potential Lipid Decontamination Technique For Life Detection Instruments, Denise Kathleen Buckner

Theses and Dissertations

In the search for extraterrestrial life, identification of molecular biosignatures is a key technique. Lipids are important molecular biosignatures: they are ubiquitous to terrestrial life, survive for billions of years in the geologic record, can form biotically and abiotically (bearing molecular features indicating biogenicity), and are detected throughout the Solar System. Lipid-based life detection instruments require stringent contamination control to prevent false positives, but traditional decontamination techniques are unlikely to sufficiently remove lipid contamination without compromising instrument materials. This thesis investigates Electron Beam Irradiation (EBI) as a potential decontamination technique; five representative lipid standards, including palmitic acid, oleic acid, heneicosane, …

Characterizing Palmitoylation On The Sodium Hydrogen Exchanger Isoform 1 (Nhe1), Moriah Hovde

Theses and Dissertations

This dissertation focuses on the role of palmitoylation in regulating the sodium hydrogen exchanger isoform 1 (NHE1) and associated cellular functions as well as how various phosphorylation pathways alter NHE1 palmitoylation. Palmitoylation is the only known reversible lipid modification, allowing for the dynamic regulation of palmitoylated proteins not found with other lipid modifications. NHE1 regulates intracellular pH (pHi) by exchanging an extracellular sodium for an intracellular hydrogen. Additionally, NHE1 is involved in regulating cell volume, cytoskeletal organization, protein anchoring, cell migration, and cell proliferation. NHE1 is highly regulated by protein interactions and modifications including phosphorylation. To begin, the first study …

Molecular Mechanisms Of Transcriptional Responses In Stress-Induced Human Cells, Sayantani Ghosh Dastidar

Theses and Dissertations

Precise regulation of gene expression is essential for maintaining cell homeostasis and survival. Rapid responses to intracellular or extracellular stimuli involve highly regulated genome-wide changes in the transcriptional landscape. However, works on transcriptional regulation thus far has been primarily focused on genes and gene promoters. Consequently, ubiquitous transcription outside of genes has been greatly neglected. Therefore, in order to understand genome-wide transcriptional changes with stimuli, we looked at transcription factor distribution, RNA Polymerase II (Pol II) dynamics, histone changes, chromatin accessibility at both promoters and promoter-distal regions along with changes in gene transcription. With this approach, we were able to …

Automated Fractional Snow Cover Monitoring From Near-Surface Remote Sensing In Grassland, Anai Caparo Bellido

Theses and Dissertations

Snow cover is an important variable in both climatological and hydrological studies because of its relationship to environmental energy and mass flux. However, variability in snow cover can confound satellite-based efforts to monitor vegetation phenology.

This research explores the utility of the PhenoCam network cameras to estimate Fractional Snow Cover (FSC) in grassland. The goal is to operationalize FSC estimates from PhenoCams to inform and improve satellite-based determination of phenological metrics.

The study site is the Oakville Prairie Biological Field Station, located near Grand Forks, North Dakota. I developed a mostly semi-automated process to estimate FSC from PhenoCam images through …

Bioenergetic Modulation Of Retinal Progenitor Cell Glycolysis To Drive Invasion For Photoreceptor Replacement, Jei Espinal

Theses and Dissertations

Given the limited regenerative capacity of the mammalian retina, cell-replacement strategies are necessary. To replace photoreceptors lost to disease or trauma and restore visual function, laboratories throughout the world are researching photoreceptors replacement strategies using subretinal transplantation of retinal progenitor cells (RPCs). A major obstacle to the advancement of photoreceptor cell-replacement strategies includes low rates of invasion and integration of transplanted cells into the host retina. Currently, there is a limited understanding of how RPC glycolysis may facilitate invasion. To further understand RPC glycolysis-driven invasion, the current investigation involved the bioenergetic modulation RPCs for glycolysis analysis and invasion modeling. The …

Surprises! Time-Tested & Easy-To-Follow Tips To Take The Mystery Out Of Success For Middle And High School Readers, Morose Leonard

Theses and Dissertations

Surprises! encapsulates my education at Lehman College. During my three years of studies, I have developed a greater appreciation for science (both natural and social), and I understand science's fundamental philosophy, which at its core denotes humanity’s effort to demystify the workings of the universe, of which we are a part. If we allow it, science can dispel our misconceptions and prejudices about ourselves and our place in the universe.

Through Surprises!, I have sought to apply that understanding to help promote our human aspirations without disrupting the delicate balance of life as we know it. For better or …

Sinorhizobium Meliloti Changes Motility Phenotypes In The Presence Of Serum, Eris K. Bisal

Theses and Dissertations

Sinorhizobium meliloti is a nitrogen-fixing flagellated soil bacterium that engages in a mutual symbiotic relationship with the legume Medicago sativa. Using its flagella, S. meliloti is chemotactic in response to varying environmental cues thereby directed to a target, which is typically the root hairs of M. sativa. Lu et al, 2012 suggested that a signal from the plant can switch S. meliloti from a free-living flagellated microbe to a host-invading non-flagellated microbe, although this signal has not been identified. This switch is thought to occur inside curled root hairs of the plant, and after becoming non-flagellated, S. …

A National Study Of Internship Directors' Perspectives On Psychological Assessment Practices, Elizabeth Shipley

Theses and Dissertations

This study examined the perspectives of a national sample of pre-doctoral internship directors regarding psychological testing and assessment practices at the internship level. Study participants were from member programs of the Association of Psychology Postdoctoral and Internship Centers (APPIC). The study's goals were to better understand the current state of affairs regarding the role of assessment in internship programs and to develop findings that may be useful to academic training programs and graduate students in psychology. A 32-item questionnaire was developed which inquired about general internship program information, types of assessment instruments used at each internship setting, the form and …

Best Teaching Practices To Increase Student Interest In Stem Subjects, Talar Gullapyan

Theses and Dissertations

More than ever, jobs at all levels, not just for scientists, are requiring STEM knowledge and specific abilities that are associated with a STEM education (Lacey & Wright, 2009; Rothwell, 2013). Additionally, The Bureau of Labor and Statistics (Vilorio, 2014) reports that STEM occupations will continue to grow faster than other occupations. While the demand for jobs in STEM fields continues to grow, many of these positions remained unfilled due to an unskilled labor force. Students in the U.S. also pursue far fewer degrees in STEM subjects compared to other competitive countries (National Science Board, 2012). The results from the …

#Livingwhileblack: A Mixed-Method Exploratory Analysis Of The Social Media Documentation Of Racism Via Twitter And Interviews, Judy A. Jackson

Theses and Dissertations

African Americans have endured marginalization related to racial and cultural disparities. #LivingWhileBlack has become a unique repository of all related experiences that Black people are encountering, while performing every day, seemingly mundane tasks and activities. The #LivingWhileBlack hashtag went viral on Twitter (https://twitter.com/), allowing users, including Black Twitter (https://twitter.com/), to use the international platform to share their own Black story and experience. The three research questions that were explored included the following. First: How are profiling events related to #LivingWhileBlack events documented, reported and expressed by Social Media Users? Second: How are others/people using Twitter (https://twitter.com/); affected by various incidences …

Resource For A Brief Early Somatic Intervention To Reduce Symptoms Of Post-Traumatic Stress For Victims Of Violence Crime In Acute Hospital Settings In Southeast Los Angeles, Jamie Gamboa

Theses and Dissertations

The goal of this dissertation was to create an intervention resource guide containing recommendations that can be utilized by the South Los Angeles Trauma Recovery Center (SLATRC) to implement a brief early somatic intervention for victims of violent crime in acute hospital settings in Southeast Los Angeles. It was decided that an intervention based upon the Trauma Resiliency Model/Community Resiliency Model (TRM/CRM) would best fit the needs of the population. First, a systematic literature review was conducted to increase the efficacy of the resource by gathering information on similar interventions. The specific questions guiding this review were as follows: (1) …

Development Of A Resilience-Oriented Stress Management Group Intervention For Mothers Experiencing Homelessness, Lily Danielle Rowland

Theses and Dissertations

Family homelessness, particularly of mothers and their children, continues to remain an unfortunate epidemic throughout the United States. Personal, relational, and systemic adversity and trauma leave this population at high risk for instability, physical and mental health challenges, and impaired wellbeing. However, despite these difficulties, research strongly suggests that positive functioning can remain in families experiencing homelessness and that many go on to leave shelters and maintain stability, particularly when mothers have effective strategies to manage stress in contexts of adversity. In addition to identifying the needs of mothers experiencing homelessness, the literature also has highlighted factors of individual and …

Developing Ei: Leadership Journeys In Emotional Intelligence, Erin Bric

Theses and Dissertations

Emotional intelligence (EI) has been deemed a critical competency for leaders in today's world. However, there is little information to help OD practitioners, leaders, and organizations understand how leaders can develop these skills in ways that meaningfully and positively impact their teams and organizations. This study examined the journeys of senior leaders as they developed their emotional intelligence. The study addressed two research questions: how leaders perceive they have developed their emotional intelligence throughout their lifetimes and how leaders are continuing to develop their emotional intelligence in their lives and careers. Key themes emerged out of leader interviews and were …

Climate Change, Social Media, And Generation Z, Melanie Morris

Theses and Dissertations

Recent publications report that adults known as Generation Z, between the ages of 18 to 23, increasingly rely upon social media to gain knowledge of social issues. Given social media's embeddedness in Generation Z's life, this study sought to understand if or how social media has influenced and possibly empowered Generation Z to act on social issues, particularly global climate change. This study used a phenomenological research method, which focused on the commonality of Generation Z's lived experience. Emerging themes collected through a literature review and data portray Generation Z as maturing into adulthood as tech-savvy, diverse, and inclusive self-starters. …

Leading, Coaching, & Mentoring: A Study Of Coach-Athlete Relationships As Associated Factors In Performance, Katie D. Kamachi

Theses and Dissertations

This research focused on global leadership and change and competitive athletics. Servant leadership, including the five servant leadership variables; altruistic calling, emotional healing, wisdom, persuasive mapping, and organizational stewardship, as well coach athlete relationships, including the three coach-athlete relationship variables; closeness, commitment, and complementarity, were used to analyze athlete perceptions. This study then found correlations of the associated factors to outcome, at team and individual levels. Findings from this study include recognizing the commitment of the head coach as a factor in team mins; individual athlete perception of closeness as related to games started; the emotional healing aspects from the …

Homeless With Breast Cancer On Skid Row, Los Angeles: A Review Of Services For Women, Eva Dittrich

Theses and Dissertations

This study reviews the literature on breast cancer in general and narrows to within the context of the homeless population of Skid Row, Los Angeles, positing that the experience of breast cancer while homeless is a compounded traumatic experience with complex and numerous practical challenges. It poses the following question: What services are available to homeless women on Skid Row, Los Angeles, the information about which can be found online? Which and how many services are dedicated to breast cancer screenings and treatment? Are other types of cancer screening and treatment services advertised on the included service organizations' websites? A …

Advancing Leadership Consciousness: Integrity From The Inside Out, Jessica Plancich Shinners

Theses and Dissertations

A poll conducted by the World Economic Forum (2015) found that 86% of respondents perceive that we are facing a global leadership crisis. At the time of this study, the COVID-19 pandemic has impacted the global ecology in dramatic and widespread ways. In light of this uncertain political, economic, sociological, and ecological climate, humanity is in need of consistent and reliable ways of developing exemplary leaders. As society enters into massive technological advancement, leaders and collaborators are at risk of obsolescence if we do not find innovative ways to harness innate human capacities to advance consciousness and co-evolve with technology. …

Plant-Pollinator Associations In An Eastern Serpentine Savannah And The Effects Of Overbrowsing, Allyson Richins

Theses and Dissertations

Chapter 1: Native plant response to deer overbrowsing in a serpentine savannah

Plants are particularly vulnerable to physical disturbance in low productivity areas, due to a high cost of replacing lost plant tissue. In the eastern United States, serpentine grasslands are fragmented ecosystems with high concentrations of rare endemic plant species, low concentrations of soil nutrients, and uncontrolled deer overpopulation. This study assessed functional responses of native angiosperms in a rare eastern serpentine savannah to selective deer browsing. Plant count, flower count, floral area, vegetative area, and plant height of 10 serpentine plant species were compared inside and outside of …

Community Reentry And Juvenile Justice: The Role Of Developmental Science In Legislative Decision-Making, Kristina A. Mcguire

Theses and Dissertations

The goals of the present study were to examine federal and selected state legislation for reentry initiatives within the juvenile justice system using content analysis, including determining if bill language utilized terminology reflecting research or developmental science. Thematic analysis was used to examine publicly available federal documents focused on juvenile justice reentry to understand how policymakers were promoting reentry initiatives. Federal bills and documents from April 1, 2008 to December 31, 2019 and state bills from January 1, 2015 to December 31, 2019 for Virginia, North Carolina, Iowa, Missouri, Maryland, New Jersey, Kansas, and Georgia that referenced juvenile justice and …

The Effects Of Gspt1 Degradation On Serum Calcium, Parathyroid Hormone, And Fibroblast Growth Factor 23 Concentrations In Human Cereblon Knock-In Mice, Kamran Ghoreishi

Theses and Dissertations

Test article CC-325 is a potent oral cereblon (CRBN) modulator that has shown potent G1 to S phase transition 1 (GSPT1) degradation and anti-tumor activity in pre-clinical models. One of the adverse effects associated with CC-325 was dose dependent hypocalcemia, which was determined to be an on-target toxicity.

To investigate the mechanism of hypocalcemia, we conducted a toxicity study in human cereblon (huCRBN) knock-in (KI) mice with CC-325. The huCRBN KI mice are transgenic mice engineered to express human cereblon that is capable of binding to CRBN and degrading GSPT1. Four groups of mice were treated with vehicle (0 mg/kg), …