Digital Commons Network^™

The Role Of Autophagy And Senescence In The Responses Of Non-Small Cell Lung Cancer Cells To Chemotherapy And Radiation, Nipa H. Patel

Theses and Dissertations

Cancer-associated deaths account for the second-highest mortality rates in the United States. Primary modalities of treatment often include surgery, radiation, and chemotherapy, and may also incorporate targeted therapy and immunotherapy. However, resistance to these treatments remains high, resulting in disease reoccurrence and poor survival rates. While apoptosis or cell death of tumor cells is the ideal outcome for anti-cancer therapy, this is often not the case, and in fact cancer cells may upregulate several pathways, such as autophagy and senescence, as a means to undergo alternative cell fate and evade apoptotic cell death. An essential tumor suppressor gene, TP53, …

Bisphosohoglycertae Mutase: A Potential Target For Sickle Cell Disease, Anfal S. Aljahdali

Theses and Dissertations

Bisphosphoglycerate mutase (BPGM) is a part of the erythrocyte glycolysis system. Specifically, it is a central enzyme in the Rapoport-Leubering pathway, a side glycolytic pathway involved in the regulation of the concentration of the natural allosteric effector of hemoglobin (Hb), 2,3-bisphosphoglycerate (2,3-BPG). BPGM catalyses the synthesis and hydrolysis of 2,3-BPG through its synthase and phosphatase activities. The synthase activity is the main role of BPGM, while the phosphatase activity is low and is activated by the physiological effector, 2-phosphoglycolate (2-PG) with the latter mechanism poorly understood.

BPGM activity and 2,3-BPG levels in red blood cells (RBCs) have a significant role …

Learning-Based Predictive Control Approach For Real-Time Management Of Cyber-Physical Systems, Roja Eini

Theses and Dissertations

Cyber-physical systems (CPSs) are composed of heterogeneous, and networked hardware and software components tightly integrated with physical elements [72]. Large-scale CPSs are composed of complex components, subject to uncertainties [89], as though their design and development is a challenging task. Achieving reliability and real-time adaptation to changing environments are some of the challenges involved in large-scale CPSs development [51]. Addressing these challenges requires deep insights into control theory and machine learning. This research presents a learning-based control approach for CPSs management, considering their requirements, specifications, and constraints. Model-based control approaches, such as model predictive control (MPC), are proven to be …

Glycosaminoglycan Modulation Of Key Proteins In Alzheimer's Disease, Tamim Chiba

Theses and Dissertations

Glycosaminoglycans (GAGs) are linear polysaccharides whose disaccharide building blocks consist of an amino sugar (D-glucosamine) and either uronic acid (D-glucuronic acid or L-iduronic acid) or galactose. Nearly all GAGs (with the exception of hyaluronic acid) are covalently attached to proteins, forming proteoglycans, and are ubiquitous in nature. The monomeric units, chain length, degree and pattern of sulfation, and epimerization are important properties that determine the influence of these molecules. Through Coulombic forces, hydrogen bonds and hydrophobic interactions, they regulate many physiological and pathophysiological processes such as blood coagulation, cell growth and differentiation, inflammatory processes, host defense and viral infection mechanisms. …

Regulation Of Osseous Healing By Macrophage-Derived Wnt Ligands, Jefferson Overlin Abaricia

Theses and Dissertations

Osseous healing is regulated by various classes of cells and signaling mechanisms. Of these, Wnt signaling has long been known to be critically important in directing the differentiation of mesenchymal stem cells (MSCs) into osteoblasts during bone healing. However, the source of these Wnt ligands is not known. Recently, a body of literature has arisen finding both beneficial and detrimental effects of macrophage-derived Wnt ligands on the regeneration of other tissues (liver, kidney, heart, and intestine), often by regulating inflammatory activation and stem cell behavior. As macrophages have been well-characterized as orchestrators of healing, their use of Wnt signaling to …

Intermittent Theta Burst Stimulation: Application To Spinal Cord Injury Rehabilitation And Computational Modeling, Neil Mittal

Theses and Dissertations

Loss of motor function from spinal cord injuries (SCI) results in loss of independence. Rehabilitation efforts are targeted to enhance the ability to perform activities of daily living (ADLs), but outcomes from physical therapy alone are often insufficient. Neuromodulation techniques that induce neuroplasticity may push the limits on recovery. Neuromodulation by intermittent theta burst transcranial magnetic stimulation (iTBS) induces neuroplasticity by increasing corticomotor excitability, though this has most frequently been studied with motor targets and on individuals not in need of rehabilitation. Increased corticomotor excitability is associated with motor learning. The response to iTBS, however, is highly variable and unpredictable, …

Stat5a Regulation By Serine Phosphorylation In Breast Cancer, Alicia E. Woock

Theses and Dissertations

The neuroendocrine hormone prolactin (PRL) and its cognate receptor (PRLr) have been implicated in the pathogenesis of breast cancer. PRL signaling relies on activating kinases such as the tyrosine kinase Jak2 and serine/threonine kinases ERK1/2, NEK3, PI3K, and AKT. In the canonical pathway of PRL signaling, JAK2 phosphorylates the transcription factor STAT5a at tyrosine residue 694 (pY694-STAT5a), preceding STAT5a nuclear translocation and transcriptional activity. However, STAT5a exists with functional duality as a transcription factor, having both pro-differentiative and pro-proliferative target genes. Other STAT family members (STATs 1, 3, and 6) have been shown to have transcriptional activity in the un-tyrosine-phosphorylated …

The Student Experience Of Higher Education During The Covid-19 Pandemic: How Class And Race Shape Socially Distanced Learning At A Public University, Alice M. Quach

Theses and Dissertations

While prior research has examined trends toward growing uncertainty and precarity for young adults within the economy and higher education, the COVID-19 pandemic appears to be exacerbating these existing inequalities and risks. Drawing from 30 semi-structured in-depth interviews with college students at a large Southern and urban public university, this study examines how the COVID-19 pandemic is affecting students’ learning experiences, and how those experiences are differentially shaped, in turn, by their class and race. This exploratory research illuminates our understanding of the multiple mechanisms by which the COVID-19 pandemic is shaping existing inequalities in the higher education system, mostly …

North Dakota's New Frontier: Unmanned Aircraft, Matthew D. Dunlevy

Theses and Dissertations

Unmanned Aircraft, or drones, represent a modern-day frontier, one that is, as yet, neither fully explored nor fully developed. The state of North Dakota has moved into the frontier and is deeply involved in its exploration and development. The state has invested tens of millions of dollars in the Unmanned Aircraft Systems (UAS) industry, an investment that has helped to attract tens of millions of additional dollars from military sources and from private business firms. The University of North Dakota’s John D. Odegard School of Aerospace Sciences, long-recognized as among the leading aviation schools in the nation, is at the …

Development Of Metal-Containing Nanoparticles For Chemical Analysis And Bioanalysis, Juan Han

Theses and Dissertations

Metal-containing nanoparticles (MCPs) have been applied in fields ranging from environmental monitoring to biomedicine. This breadth is due to the outstanding behavior of MCPs as catalysts and imaging agents, and the ease with which nanoparticle morphology, composition, and reactivity (such as agglomeration) can be controlled. The work described in this dissertation will have two fundamentally different foci that are both essential for further development of MCPs as tools for chemical and bioanalysis. The first focus is on particle-by-particle characterization MCPs and the second focus is on creation of new composite MCPs. A total of four projects are included in this …

Eating Disorder Symptoms On Female Collegiate-Level Athletes And Non-Athletes, Kennedy Victoria Kidd

Theses and Dissertations

In previous research, it found that the risk of developing an eating disorder (ED) is much higher in females compared to males (Dunca, Ziobrowski & Nicol, 2017; Raevuori, Keski-Rahkonen, & Hoek, 2014; Lewinsohn, Seeley, Moerk, & Striegel-Moore, 2002). Similarly, research has shown that the prevalence of EDs is much higher in female athletes compared to male athletes (Johnson, Powers, & Dick, 1999). Despite the risk of developing an ED being higher in females, research has not provided a strong understanding of the prevalence of EDs comparing female student-athletes and female student non-athletes. There is research that shows that athletes may …

Concentration-Streamflow Relationship In The Red River To North Near Grand Forks, North Dakota, Kabamba Nancy Ngoyi

Theses and Dissertations

For the last 15 years, the nutrient concentrations have fluctuated in both particulate and dis-solved forms. The increasing concentration of sulfate and chloride in the North Great Plain Basin impose threats to water quality in the Basin. During this spring snowmelt season of 2021, water samples were collected three times daily in the Red River at Grand Forks, North Dakota to de-velop a concentration streamflow relationship for sulfate, nitrate, chloride, and fluoride. The 2020-2021 winter was very dry as a result the snowmelt streamflow was very low, the rising limb period was short, but the streamflow increased rapidly from 75 …

Transcriptomic And Pharmacological Characterization Of A Murine Line Harboring An Antidepressant-Insensitive Serotonin Transporter, Meghan Alicia Rodriquez

Theses and Dissertations

Perinatal depression is a complex mood disorder often treated with Selective Serotonin Reuptake Inhibitor (SSRI) antidepressants, which block the serotonin transporter (SERT) and alter synaptic serotonin concentrations. The serotonergic system is highly plastic and serotonin regulates the growth and maturation of neurons and signaling systems from conception through end of life. Notably, interference with serotonin levels during an SSRI sensitive window (SSW) of development in humans and rodents has been linked to neurovegetative effects which increase susceptibility for the manifestation of anxiety- and depressive-like behaviors and can be persistent throughout life. We hypothesize the persistent behavioral perturbations resulting from acute …

Student-Athlete Motivation And Gender, Jessica Schanilec-Gowan

Theses and Dissertations

Billions of dollars are spent each year on athletic teams and the education of National Collegiate Athletic Association (NCAA) student-athletes. Successful athletic programs require teams that are excelling in the classroom and also within their sports. Stakeholders are continually searching for factors that impact the academic success of the student-athlete population. This study explored one of those factors: motivation. In this research, an ex post facto quantitative research format was utilized to determine if there were relationships among three subscales of Gaston’s (2002) Sports and Academics Questionnaire (SAMSAQ) and gender identity. More specifically, the three SAMSAQ subscales that were examined …

Role Of Schlafen 12 In Lung Adenocarcinoma And Triple Negative Breast Cancer, Sarmad Al-Marsoummi

Theses and Dissertations

Schlafens are a family of genes expressed in a wide range of mammals, including rodents and humans. Schlafens are grouped based on molecular structure and size into the short, intermediate, and long Schlafen families. Mice express all three families of Schlafens; in contrast, humans express only long and intermediate Schlafens. Schlafens have diverse functions, including cell proliferation, cell differentiation, immune cell growth and maturation, viral replication, cancer cell biology, and chemosensitivity. Our studies have focused on Schlafen 12 (SLFN12). SLFN12 is an intermediate Schlafen, it is expressed in humans but not in rodents, and is localized to the cytoplasm. SLFN12 …

Giving Birth On Foreign Soil: A Phenomenological Study Of Somali Women Giving Birth In North Dakota And Minnesota, Tracy Jo Enger

Theses and Dissertations

Health disparities continue to exist in minority populations in the United States. People ofcolor have a higher incidence of negative birth outcomes; and view their experiences with less satisfaction. The purpose of this study was to explore what Somali Muslim have women have experienced during their labor and delivery in Minnesota and North Dakota. This research was conducted to improve our understanding of the needs of Somali women during labor and delivery in order to provide nursing care that is culturally sensitive. Phenomenological data was collected from personal interviews with Somali women who had given birth in North Dakota and …

Developing Computer Aided Algorithms For Automatic Assessment Of Psoriasis Disease Severity Using Digital Images, Intisar Rizwan I Haque

Theses and Dissertations

The purpose of the research work was to develop image processing algorithms for automatic and objective assessment of psoriasis to aid in more effective management and treatment of patient symptoms. For this purpose, multiple classical machine learning-based unsupervised approaches are proposed which achieve a maximum average accuracy of ~94% on a dataset of 45 images of various resolutions. This accuracy improves performance by 14% in comparison to existing approaches reported in the literature on the same dataset of images. An alternative deep learning neural network-based approach was proposed that achieves an average accuracy of ~87% for the same set of …

Identifying Characteristics That Lead To Better Academic Performance For International Students In U.S. Colleges, Hadi Rajabbeigi

Theses and Dissertations

A large proportion of students in the United States is international students. Thus, educational leaders need to facilitate the learning process for this population. This study investigated the characteristics of international students that affect their academic performance. The purpose of this study was to determine how individual characteristics of international students affect the learning strategies they choose and how it affects their academic performance. For this purpose, the survey was designed according to the literature, which measures characteristic variables based on VIA character strengths (Peterson & Seligman, 2004), learning strategies (MSLQ) developed by Pintrich et al. (1991), and academic performance …

Development Of A Resource Manual For Working With Latino Parolees, Abner Flores

Theses and Dissertations

The standard psychological treatment available for Latino men who are newly released from prison has remained unchanged for over thirty years and focuses on decreasing recidivism through cognitive-behavioral interventions by managing and decreasing law-breaking behaviors. However, clinical experience with inmates suggests that personal experiences, cultural values, and intergenerational trauma along with the long-term impact of poverty, gang ties, and repeated incarceration will all influence post-release behavior. Understanding these areas could help draw out protective factors and innate resiliency, therefore informing treatment and helping newly released inmates from becoming repeat offenders. In this dissertation, I developed a resource manual for service …

The Harm In Seeking Care: Assessing The Relationship Between Healthcare Discrimination And Healthcare Avoidance Behaviors In The Past Year And Since The Start Of The Coronavirus Pandemic In A Transgender And Gender Independent Sample, Kyle L. Mason

Theses and Dissertations

Background: Gender minorities encounter a myriad of barriers to accessing general and gender affirming healthcare. Financial disparities impacting affordability of healthcare costs and insurance-based denials for gender-affirming care are among prominent barriers discussed. Considerations of the prevalence of stigma, discrimination, and erasure of gender minority identities must not be neglected when seeking to understand healthcare accessibility and utilization in this population. Previous researchers have examined gender minority patients’ experiences of discrimination in healthcare settings and delaying care due to fear of discrimination. There is a dearth of knowledge about the relationship between lifetime exposure to varied forms of healthcare discrimination …

Nanopore Detection Of Long Time-Scale Single Metallic Cluster Conformational Changes And Surface Ligand Exchange, Bobby D. Cox

Theses and Dissertations

Single molecule nanopore spectroscopy serves as an emerging tool for studying small molecules in aqueous environments without the need for additional chemical labels. Recently it has been seeing increased use as a tool for investigating small metallic nanoparticles and clusters. These clusters consist of a handful of metallic atoms (e.g. Au18) and are regularly ‘passivated’ with organic ligands to provide functionality and protect against aggregation. There has been a widening gap in research studying the surface kinetics of these passivating ligands at the single molecule level as well as during the exchange process. In this work we investigated these surface …

A Mixed Methods Approach To Examine Racial Disparities In Adherence To Surveillance Mammography Among Breast Cancer Survivors, Megan C. Edmonds

Theses and Dissertations

PURPOSE: Annual surveillance mammography is a critical part of routine cancer care for breast cancer (BC) survivors; reducing mortality by 39%. However, disparities exist with regard to adherence to surveillance mammograms among BC survivors; Black women are 44% less likely to adhere than their White counterparts. Despite the existence of this racial disparity for over a decade, little is known about factors that explain reasons for Black BC survivors’ non-adherence. This is because most studies have not evaluated the role of healthcare delivery or psychosocial factors. Guided by the Behavioral Model for Vulnerable Populations, the purpose of this mixed methods …

The Therapeutic Potential Of Glycosaminoglycan Directed Metalloglycomics: Elucidating New Targets And Mechanisms For Polynuclear Platinum Complexes, James D. Hampton

Theses and Dissertations

Cancer remains a serious clinical problem and in order to continue to improve patient health outcomes, novel treatment strategies must continue to be developed. Platinum chemotherapies remain as one of the most widely successful classes of cancer therapeutic, with over 50% of cancer patients receiving a platinate at some point over the span of their treatment. However, intrinsic and extrinsic resistances limit their effectiveness, long term efficacy, and broad applicability to multiple cancer subtypes. Therefore, the development of new agents and strategies which can overcome these limitations are crucial for patient health. To this end a novel class of polynuclear …

Methods For Developing A Machine Learning Framework For Precise 3d Domain Boundary Prediction At Base-Level Resolution, Spiro C. Stilianoudakis

Theses and Dissertations

High-throughput chromosome conformation capture technology (Hi-C) has revealed extensive DNA looping and folding into discrete 3D domains. These include Topologically Associating Domains (TADs) and chromatin loops, the 3D domains critical for cellular processes like gene regulation and cell differentiation. The relatively low resolution of Hi-C data (regions of several kilobases in size) prevents precise mapping of domain boundaries by conventional TAD/loop-callers. However, high resolution genomic annotations associated with boundaries, such as CTCF and members of cohesin complex, suggest a computational approach for precise location of domain boundaries.

We developed preciseTAD, an optimized machine learning framework that leverages a random …

An Exploration Of Teacher Residents’ Perception Of Culture And Their Use Of Culturally Responsive Pedagogy, Jodi Larson

Theses and Dissertations

Urban teacher residency (UTR) programs place residents in urban schools labeled “hard-to-staff” or “under-resourced.” Enrollment in residency schools tends to be majority Brown or Black students from various cultures. Teacher residents are from diverse backgrounds and races who have a commitment to teach in Title 1 schools with the support of a residency program that coaches them how to teach using culturally relevant pedagogy. Their journeys are unique from typical student teaching experiences because they co-teach with an experienced teacher for a full school year while attending university classes on pedagogy and theory. This qualitative case study followed seven elementary …

Navigating Success: First-Generation Pathways To Institutional Integration, Katherine B. Drumm

Theses and Dissertations

Guided pathways mobile applications are one technology-based tool that colleges and universities have implemented in an attempt to educate and guide students through the myriad steps necessary to matriculate, integrate and successfully graduate from their institution at scale. Using Bourdieu’s concepts of habitus and capital, Astin’s model of student involvement and Tinto’s model of student integration as a conceptual framework, and building upon the work of Slanger et al. (2015), this study investigated if the Educational Stress scale score from the College Student Inventory (CSI) can act as a measure of student habitus. In addition, this study used institutional data …

Modeling Substance Use And Mental Disorder Comorbidity Using Latent Variable And Network Approaches, Courtney T. Blondino

Theses and Dissertations

Introduction. Substance use disorder (SUD) is a common condition that affects millions of Americans. Addressing SUD has been complicated by comorbid mental disorders and co-occurring substance use. Consequently, detailing and addressing SUD and comorbid SUD represent an important goal to improve the health of Americans.

Objective. The research goal of this dissertation was to characterize the comorbidity between substance use, including tobacco use, and mental disorder symptoms measured as negative affect and externalizing symptoms in a population-based sample using latent variable and network approaches.

Methods. Waves 1 – 3 from the Population Assessment of Tobacco and Health Study were used. …

Single-Molecule Investigation Of I-Motif Folding And Dynamics In Bioinspired Dna Platforms, Anoja Megalathan

Theses and Dissertations

Although the canonical right-handed B-DNA is the predominant form of DNA in cells, DNA can adopt other secondary structures such as hairpins, G-quadruplexes (G4), and intercalated motifs (i-motif). These secondary structures are typically formed in the regions with repeat sequences. Among many secondary structures DNA can adopt, G-quadruplexes and i-motifs (tetraplex structures formed in the G-rich and C-rich sequences, respectively) caught significant research interest in recent years due to the prevalence of G-rich and C-rich sequences in the human genome. While G-quadruplexes are extensively studied, little is known about i-motifs – especially their formation and stability under cellular environments. For …

Genetics And Alcohol Interventions In Youth, Zoe E. Neale

Theses and Dissertations

Alcohol is the most commonly used substance among youth, and risky alcohol use is associated with harmful consequences such as accidents, academic consequences, and physical and emotional health problems. Alcohol use disorders are approximately 50% heritable, yet most efforts to prevent and intervene upon youth alcohol use focus only on environmental factors. Furthermore, current prevention and intervention programs tend to have modest effects and are not uniformly effective for all individuals. Gene-by-intervention (GxI) studies offer an opportunity to expand current understanding of interventions by examining whether underlying genetic risk may contribute to differential program effects. Much of the current GxI …

Preclinical Pharmacokinetic Studies Of Pterostilbene, And Its Human Pharmacokinetic Prediction Using Physiologically Based Pharmacokinetic Modeling, Palak S. Phansalkar

Theses and Dissertations

This dissertation research aimed to determine the preclinical pharmacokinetics of pterostilbene in vitro and develop a physiologically based pharmacokinetic (PBPK) model to simulate the animal and human PK profiles for pterostilbene. The in vitro PK characteristics of pterostilbene, including Caco-2 cell permeability, plasma protein binding, and metabolic stability were determined using rat and human intestinal & hepatic S9 subcellular fractions. The in vitro metabolic parameters determined in rat subcellular fractions were extrapolated to in vivo values to predict the oral bioavailability (F_oral) in rats. The predicted F_oral was found to be 43.0 %, which is similar to …