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Behavior Of Selective Oxygen Functional Groups Upon Hydrothermal Carbonization And Pyrolysis Of Biomass And Their Roles On Selective Applications, Nepu Saha

Theses and Dissertations

Hydrothermal carbonization (HTC) is a thermochemical process where biomass is treated in water under high temperature and corresponding vapor pressure. During HTC, biomass undergoes a series of reactions including hydrolysis, decarboxylation, dehydration, aromatization, and polymerization. As a result, a solid product is produced from biomass after HTC process, which is widely known as hydrochar. Depending on the HTC process conditions, a wide-variety of oxygen-containing groups formed on hydrochar surfaces. Many of these groups could play critical roles in applications such as adsorption, and energy and gas storage, etc. However, not all functional groups are exposed on the surface due to …

Bioink Optimization And Effects Of Microgravity On 3d Bioprinted Cell Laden Constructs, Likitha Somasekhar

Theses and Dissertations

Bioengineered 3D tissue constructs have gained attention as in vitro tools for the study of cell-cell and cell-matrix interactions and are being explored for potential use as experimental models for mimicking human tissues. One of the main problems in tissue engineering is the necessity to vascularize complex engineered tissues and sacrificial printing has been recognized as a possible solution to vascularization of the bioprinted tissues. Research studies have demonstrated that exposure to microgravity in space induces adaptive alterations in vascular structure and function. Changes in the morphology and gene expression is observed when endothelial cells are exposed to microgravity and …

A Comparative Study To Assess The Osteogenic Potential Of Competing Bioceramics For Bone Tissue Engineering, Nashaita Patrawalla

Theses and Dissertations

Biomimetic composite scaffolds comprising of bioactive ceramic-based materials incorporated within a polymeric framework have shown considerable promise for use in bone tissue engineering (BTE) applications. Although bioceramics have shown immense promise for use in BTE, few studies have performed a head-to-head comparison of the osteogenic properties of the most promising bioceramic materials. The goal of this study was to compare three different bioceramics – Bioglass 45S5 (BG), Laponite XLG (LAP), and β-Tricalcium Phosphate (TCP) – on the physical properties (i.e., swelling, stability, compressive modulus, bone bioactivity) of methacrylated collagen (CMA) hydrogels and osteogenic differentiation of human MSCs (hMSCs) encapsulated within …

The Effect Of Simulated Mangrove Forest Width On Shoreline Stabilization At Multiple Tidal Water Levels, James Edward Austin

Theses and Dissertations

Red mangrove (Rhizophora mangle) forests protect hundreds of miles of Florida’s shorelines from erosion and wave damage associated with severe storms and daily wave action. Shorelines are often protected from erosion with man-made structures such as seawalls, revetments, and breakwaters. However, these man-made structures disrupt natural ecosystems and cause scour and erosion in surrounding areas. The presence of mangroves not only prevents erosion but shelters marine life and filters water, promoting ecological health. Mangroves are now being implemented for wave energy reduction and shoreline stabilization, but there is little quantitative data about the precise sediment transport induced or prevented by …

Geographic Variability In The Reproductive Hormones And Oocyte Maturation Of Bonefish (Albula Vulpes) In The Bahamas, Ashley Yarbrough

Theses and Dissertations

Bonefish (Albula vulpes) are an extremely popular sport fish that are a vital component of the multimillion-dollar recreational flats fishery. Despite its economic importance for many countries, there is little information available about many aspects of their reproductive biology. Significant gaps include analysis of the hormones that influence oocyte development and the nutrient levels in oocytes that drive embryonic and early larval development and how these parameters vary among habitats. The goals of this study were to characterize and quantify the reproductive hormones, oocyte developmental stages, and nutrient composition of oocytes from fish at or near spawning sites across multiple …

Roles Of Holocene Climate And Pastoralism In Shaping Andean Landscapes, Courtney Renee Shadik

Theses and Dissertations

The Holocene (c. 11,700years ago –present) was a time rich in Andean climatic, environmental, and cultural changes. Assemblages were reforming after the last ice age, millennial scale wet and dry events caused the largest changes in lake level of the last 100,000 years, and humans came to dominate the landscape. Against the backdrop of ecological change, this study asks the following questions-Was the onset of camelid domestication associated with mid-Holocene aridity? Are modern, high-elevation vegetation assemblages a product of manufactured landscapes? What are the long-term effects of intense agropastoralism on paleoecological reconstructions of Andean landscapes? Did Holocene agropastoral domesticates coevolve? …

Exploration Of Antimicrobial And Cell Proliferation Properties Of Nanofibers Incorporating Nopal (O. Cochenillifera) Extract, Cristobal Rodriguez

Theses and Dissertations

This study focused on the fabrication of Forcespinning® nanofibers composed of Opuntia cochenillifera, ‘nopal’, mucilage (N) extract, chitosan (CH), and pullulan (PL) (N/CH/PL) were developed with an optimum fiber average diameter of 406±127 nm, and studied for their ability to sustain adhesion and proliferation of mouse embryonic fibroblast (NIH 3T3) cells. After a 6-day incubation period, N/CH/PL nanofibers displayed robust cell proliferation, while also exhibiting inhibitory properties through an N extract dip-coating process against gram-negative bacteria Escherichia coli in a 24 h bacterial growth study. A demonstration of integrated natural bioactive compounds with combined biodegradable polymers, provide an enhanced …

Automated Identification Of Lines In Data From Gravitational Wave Detectors, Thomas A. Cruz

Theses and Dissertations

On the frontier of gravitational wave (GW) astronomy, the LIGO detectors record vast quantities of data that need to be analyzed constantly for rare and transient GW signals. A foundational problem in LIGO data analysis is the identification of spectral line features in the Power Spectral Density (PSD) of the data. Such line features correspond to high power terrestrial or instrumental signals that must be removed from the data before any search for GW signals can take place. In this study the method developed aims to automate the extraction of the frequencies and bandwidths of the lines, treated as sharp …

Investigating Bmp7 Expression In Glioblastoma Multiforme, Yajaira Janett Macias

Theses and Dissertations

The PI3K/AKT/mTOR pathway regulates important cell processes such as growth, survival, motility, inflammation, proliferation, and apoptosis. In Glioblastoma multiforme (GBM) the PI3K/AKT/mTOR pathway is aberrant as it is almost always active. This results in the deregulation of downstream molecules and ultimately leads to cancer progression and maintenance in GBM tumors. In this study, we used RNA-sequencing to identify differentially expressed genes (DEGs) in U87MG GBM cells treated with NVP-BEZ235, a dual inhibitory drug targeting PI3K and mTOR. A total of 7,803 differentially expressed genes were identified via RNA-seq. GEPIA2 online tool was used to assess differential gene expression …

Practical Challenges Of Using Sunn Hemp As A Cover Crop In The Lower Rio Grande Valley, Qulina Rai

Theses and Dissertations

Sunn hemp (Crotalaria junceae) is an emerging cover crop with many reported benefits such as nitrogen fixation, carbon sequestration, and suppression of weeds and nematodes. Relatively little is known about appropriate ways to inoculate sunn hemp ensuring nitrogen fixation, and diseases that affect sunn hemp within the Lower Rio Grande Valley of Texas.

Four sticking agents, peanut oil, jaggery, gum arabic, and water used to aid inoculation of Bradyrhizobium japonicum in sunn hemp was checked on nodulation. Water treatment was least effective. Peanut oil and jaggery treatment showed better performance in overall total nodules, and active nodules. Gum …

A N-E-W (Nutrient-Energy-Water) Synergy In A Bioelectrochemical Nitritation Anammox Process, Umesh Ghimire

Theses and Dissertations

Partial nitritation combined with the anaerobic ammonium oxidation (Anammox) process offers a way of replacing the conventional nitrogen removal process of nitrification-denitrification, lowering the need for oxygen and chemical input, as well as reducing the production of sludge. However, as a by-product of the biochemical reaction driven by anammox bacteria, it produces nitrate-nitrogen (NO3- - N) (16-26% nitrogen removed), which is problematic. Microbial desalination cells (MDCs) are a promising technology capable of converting biodegradable organics into electricity (by electroactive bacteria), providing for simultaneous desalination, and wastewater treatment. Despite being a promising technology, MDCs have limitations. The first-proof of-concept of MDC …

Analysis Of Surface Water For Irrigation In The Big Sunflower River Watershed, Meredith Lynn Brock

Theses and Dissertations

Exploitation of groundwater and excess nutrient runoff are major issues plaguing agriculture and streams in the Lower Mississippi River Basin, and increased irrigation intensity has yielded a proportionate increase in water use. Quantifying the use and effects of conservation practices like on-farm water storage (OFWS) systems will justify continued adoption of these practices to mitigate groundwater decline and nutrient runoff. Since 2010, over 795 hectares of surface water storage has been added in the Big Sunflower River Watershed, and analysis of aquifer saturation shows a recent rise in the water table and a decrease in seasonal water table drawdowns. Modeling …

Metacognitive Self-Regulated Learning Processes In Computer And Print Reading Assignments Among Elementary Students In Grades 2-5, Katerina Sergi

Theses and Dissertations

Self-regulated learning (SRL) and higher-order thinking (metacognitive processes) are important in education because they contribute to effective learning and improved academic performance. These processes may be facilitated by the implementation of computer technology in the classroom. This research project examined the use of computer technology among elementary school students and possible effects on self-regulated learning and metacognitive processes, including the ability to plan, monitor, evaluate one’s own work, and apply specific learning strategies. Two main research questions were investigated: (1) Do elementary school students demonstrate SRL metacognitive processes when they use computers and paper-pencil for reading-relating tasks, and what are …

Implementation Of Second-Order Correlation Spectroscopy (Socos) Via All- Gaussian Coherent Stokes And Anti-Stokes Raman Scattering, Supriya Nagpal

Theses and Dissertations

Powerful spectroscopic techniques increasingly involve nonlinear processes that arise due to the convolution of more than one electric field - input laser pulse. Analyzing the output of optical processes like these demands the utilization of deterministic improvement tools. Three-color coherent Raman scattering represents a complex non-degenerate four wave mixing process that includes contributions from both resonant and non-resonant interaction of the three input fields to generate a signal. In order to quantify these contributions, effective differentiation of the non- resonant (background) from the resonant (coherent signal) is required. These contributions can be differentiated based on how the molecular vibrational modes …

Studies On Common Viral And Bacterial Pathogens Of Bovine Respiratory Disease During In Vitro Co-Infection, Caitlyn Cowick

Theses and Dissertations

Bovine Respiratory Disease Complex is a multifactorial disease affecting cattle worldwide resulting in high mortality and morbidity rates in the cattle farming industry. This complex is caused by multiple viral and bacterial pathogens such as Bovine Herpesvirus-1, Bovine Respiratory Syncytial Virus, Mannheimia haemolytica, and Pasteurella multocida; two of the main contributors to the initiation of this disease are Bovine Herpesvirus-1 and the bacteria, Mannheimia haemolytica. Together, these microbes co-infect immunocompromised cattle during times of increased stress and induce a severe pneumonic response along with other health complications. Research has been primarily focused on these microorganisms individually or their effect on …

Symbiosis Components Function To Inhibit Endoparasitic Nematode Infection, Rishi Khatri

Theses and Dissertations

An analysis of Glycine max homologs of the symbiosis genes DOES NOT MAKE INFECTIONS (DMI) DMI1, DMI2 and DMI3 was carried out as it relates to the defense response to Heterodera glycines parasitism. Transgenic analyses of the DMI1, DMI2 and DMI3 for overexpression showed decreased H. glycines parasitism while the analyses for RNAi showed increased H. glycines parasitism. The combination of decreased parasitism in the H. glycines-susceptible genotype G. max [Williams 82/PI 518671] and increased parasitism in the H. glycines resistant genotype G. max [Peking/PI 548402] is taken as the genes function in the defense process at some level. Prior …

Phenology And Management Of Triploid Flowering Rush (Butomus Umbellatus L.), Lee Gray Turnage Jr.

Theses and Dissertations

Flowering rush (Butomus umbellatus L.) is a perennial wetland/aquatic plant that is native to Eurasia but has invaded North America and spread across Southern Canada and the Northern U.S. where it thrives along wetlands, shallow shorelines, and in submersed habitats of lakes, ponds, streams, rivers, and reservoirs. Little is known about the life cycle of flowering rush in its invaded range as only one study has investigated flowering rush phenology in Minnesota, USA. As flowering rush continues to expand its range southward in the U.S. it is imperative that resource managers 1) better understand the plants life cycle, 2) identify …

A Functional Analysis Of The Glycine Max H2a.Z9 Gene Family In Relation To Defense To Heterodera Glycines Parasitism, Sudha Acharya

Theses and Dissertations

The histone 2A (H2A) variant Z (H2A.Z) regulates gene expression, replacing H2A predominately at +1 nucleosomes. Whereas, H2A.Z can act as either a positive or negative transcriptional regulator, this research focuses on its transcriptional activation role. This thesis focuses on examining the Glycine max HTA9 gene family (H2A.Z9). The Arabidopsis thaliana HTA9 protein sequence was used to query the G. max proteome resulting in identification of five H2A.Z9 (H2A.Z9-1-5) paralogs. H2A.Z9-1, H2A.Z9-2, H2A.Z9-4, and H2A.Z9-5 are expressed within Heterodera glycines-parasitized root cells undergoing defense. All 5 paralogs, including H2A.Z9-3, were studied in transgenic-functional analyses. Data demonstrates that H2A.Z9 overexpression leads …

Impact Of Altered Polyamine Metabolism On Streptococcus Pneumoniae Capsule, Moses Babatunde Ayoola

Theses and Dissertations

This dissertation is a compilation of published works and a manuscript that seek to understand the possible role of polyamines in the regulation of capsule in Streptococcus pneumoniae (Spn, pneumococcus). Spn remains a major health risk worldwide while the capsule is widely recognized as the principal virulence factor. Polyamines on the other hand are small hydrocarbon molecules known to regulate a number of cellular processes in bacteria. This work investigates the impact of deletion of polyamine biosynthesis gene, SP_0916 (cadA, lysine decarboxylase at the time of first and second publication), on protein expression and the capsule biosynthesis of virulent pneumococcal …

Polymorphism Of Wrky Factors In Corn Inbred Lines Carrying Aflatoxin Resistance Quantitative Trait Loci, Akanksha Awasthi

Theses and Dissertations

Aflatoxin is a highly carcinogenic secondary metabolite produced by Aspergillus flavus contributing to significant loss of corn production. There are several breeding techniques to make the corn resistant to aflatoxin accumulation with the genotype having desirable characters. Corn- resistant inbred line Mp313E provides resistance against A. flavus. Near-isogenic lines containing QTLs were developed by backcrossing Mp313E (resistant parent) and Va35 (susceptible parent). WRKY transcription factors play an important role in the growth and development of plants. Searching DNA markers associated with these WRKY genes is a considerable approach for the plant breeders. In this study, PCR and agarose gel electrophoresis …

Evaluation Of The Rainfastness Of Selected Insecticides In Cotton, Sara Inez Barrett

Theses and Dissertations

Rainfastness of insecticides is an understudied aspect of agricultural research. Little is known about the residual of commonly used products for key pests of cotton, as well as their residual after a rainfall event. This project was designed to evaluate the impact of rainfall on the performance of commonly used insecticides for tobacco thrips, Frankliniella fusca (Hinds); tarnished plant bug, Lygus lineolaris (Palisot de Beauvois); and bollworm, Helicoverpa zea (Boddie), management in cotton. Laboratory and field experiments suggest that rainfall occurring within 16 hours after application had a negative impact on the performance of most insecticides. Chemical analyses of leaf …

Multispectral In-Field Sensors Observations To Estimate Corn Leaf Nitrogen Concentration And Grain Yield Using Machine Learning, Razieh Barzin

Theses and Dissertations

Nitrogen (N) is the most critical fertilizer applied nutrient for supporting plant growth. It is a critical part of photosynthesis as a component of chlorophyl, hence it is a key indicator of plant health. In recent years, rapid development of multispectral sensing technology and machine learning (ML) methods make it possible to estimate leaf chemical components such as N for predicting yield spatially and temporally. The objectives of this study were to compare the relationships between canopy reflectance and corn (Zea mays L.) leaf N concentration acquired by two multispectral sensors: red-edge multispectral camera mounted on the Unmanned Aerial Vehicle …

Insecticide Resistance Monitoring And Sublethal Effects Of An Insect Growth Regulator On Tarnished Plant Bug (Hemiptera: Miridae), Beverly D. Catchot

Theses and Dissertations

Efforts to implement integrated pest management (IPM) strategies and improving our understanding of existing strategies are keys to achieving adequate control of tarnished plant bug, Lygus lineolaris. Insecticide applications are a part of IPM and monitoring their efficacy is critical for producers to remain profitable. Resistance to imidacloprid, thiamethoxam, and sulfoxaflor was documented, although there continues to remain variability among populations within the region. The insect growth regulator, novaluron, although only lethal to nymphs, plays an important role in management of Lygus. Sublethal impacts to adult plant bugs may be an important factor in reducing populations within the growing season. …

Effects Of Washing Units Of Canine Red Blood Cells On Storage Lesions, Ashley Coll

Theses and Dissertations

In humans, washing stored blood products prior to transfusion reduces storage lesions and the potential for transfusion reaction, but the effectiveness of washing units of canine whole blood is unknown. The objective of this study was to determine if a manual method of washing of stored whole blood units reduced storage lesions without adversely affecting erythrocytes. Units of canine whole blood were stored for 28 days and manually washed three times with sterile .9% NaCl. Following the first wash, there was a decrease in serum potassium (P<.0001), lactate (P<.0001), pH (P=.0110), pCO2 (P<.0001), TCO2 (P<.0001), arachidonic acid (P<.0001), and thromboxane B2 (P=.0417), and increases in iCa (P=.0494), iMg (P=.0024), MCV (P<.0001), MCHC (P=.0093), RDW (P=.0009), hemoglobin (P=.0011), and MCF (P=.0006). No bacterial growth was identified on the post-transfusion samples. Manual washing of stored blood significantly reduces storage lesions after a single wash and additional washing may cause in vitro hemolysis.

Development Of Cell Cultures From The Tissues Of Ictalurid Catfish And Investigation Into The Pathogenesis Of Blue Catfish Alloherpesvirus, Vandana Dharan

Theses and Dissertations

Lack of host-specific cell cultures necessitated initiation of primary cell cultures from ictalurid catfish. Cell cultures from the fin tissues of hybrid catfish ( channel catfish x blue catfish) were developed, characterized, and species of origin molecularly authenticated. Blue catfish alloherpesvirus (BCAHV) is an Ictalurid herpesvirus. When BCAHV was inoculated onto various fish cell lines from different families, the cytopathic effects were restricted to cell lines from family Ictaluridae indicating the host-specificity of virus. A virus challenge using channel, blue, and hybrid catfish indicated mortality due to BCAHV was significantly higher in blue and hybrid catfish. Crowding influenced BCAHV pathogenesis …

Estimation Of Wild Pig Damage To Corn Production In The Mississippi Alluvial Valley, David H. Foster

Theses and Dissertations

Wild pigs are known to be an agricultural pest, but few studies have been done to quantify wild pig damage to corn and the landscape characteristics that are conducive to this damage. In the Mississippi Alluvial Valley, only 15 of the 125 sampled corn fields were damaged (12%). Using yield estimates, input costs and hectares of crop lost, this study concluded that wild pigs cause US$1,753 of damage per hectare. The proportion of corn that was planted within selected buffer scales consistently had a negative effect on whether a field would be damaged. The proportion of soybean planted within the …

Evaluating The Management Of Bollworm (Lepidoptera: Noctuidae) In Cotton, Michael Cade Francis

Theses and Dissertations

In field experiments, thresholds, spray timings, and bollworm oviposition were conducted to evaluate the current recommended action thresholds for bollworm (Lepidoptera: Noctuidae) in cotton. Based on studies conducted evaluating thresholds, insecticide applications varied across Bt technologies. Bollgard 3 required less sprays when compared to Bollgard II and non-Bt. A second experiment highlighted the importance of timely insecticide applications for managing bollworm populations in Bollgard II cotton based on insect damage. The highest yields were associated with insecticide applications made during the timeframe that cotton is the most susceptible to bollworm damage. The last experiment was conducted to determine if oviposition …

Modified Biochar Adsorbents For Aqueous Contaminant Remediation, Herath Mudiyanselage Nimeshika Amali Herath

Theses and Dissertations

Continuous population growth and rapid industrial advancement and development have paved the way for ever increasing environmental pollution. At present, water pollution is a serious global issue that threatens environmental sustainability. The contamination of aquatic bodies with potentially toxic organic and inorganic substances are the result of world-wide anthropogenic activities. These pollutants can have detrimental health consequences on humans and ecosystems. Over the past decades, techniques such as chemical precipitation, ion-exchange, adsorption, membrane filtration, and electrocoagulation-flocculation have been developed and employed for the treatment of drinking and wastewater. Among the currently available techniques, pollutant removal by adsorption is most promising …

Developing And Applying Precision Animal Farming Tools For Poultry Behavior Monitoring, Guoming Li

Theses and Dissertations

Appropriate measurement of broiler behaviors is critical to optimize broiler production efficiency and improve precision management strategies. However, performance of different precision tools on measuring broiler behaviors of interest remains unclear. This dissertation systematically developed and evaluated radio frequency identification (RFID) system, image processing, and deep learning for automatically detecting and analyzing broiler behaviors. Then different behaviors (i.e., feeding, drinking, stretching, restricted feeding) of broilers under representative management practices were measured using the developed precision tools. The broilers were Ross 708 in weeks 4-8. The major findings show that the RFID system achieved high performance (over 90% accuracy) for continuously …

Evaluation Of Nitrapyrin As A Potential Nitrification Inhibitor In Mississippi Rice Production, William Jeffrey Mansour

Theses and Dissertations

Urea is the predominant ammonium-forming nitrogen (N) source applied in delayed-flood rice because of its high N content (46%) and relatively low cost. Nitrogen applied prior to flooding can be lost by multiple mechanisms such as ammonia volatilization or nitrification/denitrification. In recent years, technological advancements have provided alternative enhanced efficiency fertilizer additives with potential to be incorporated in rice production to reduce N losses. Research was conducted at the Mississippi State University Delta Research and Extension Center from 2018 to 2020 to determine the effects of broadcast applications of nitrapyrin at two specific growth stages to enhance fertilizer-N recovery efficiency, …