Digital Commons Network^™

Biotic Characteristics Of Managed And Unmanaged Coastal Dunes In The Outer Banks, North Carolina, Andrew E. White

Theses and Dissertations

Under future climate change and sea level rise scenarios, Natural and Nature-Based Features (e.g., dunes) that protect coastal habitat and infrastructure will be exposed to increased wave energy and storm surge. Understanding how these forces will impact coastal dunes is necessary for their continued use as protective features. Coastal dunes develop through feedback between vegetation and sediment deposition, a process complicated by species-specific growth rates and responses to burial. Wave flume studies have tested the effects of dune vegetation on erosion and found multiple plant organs across several functional types to be important for resisting erosion. Although dune building and …

A Thematic Analysis Of African American Fathers' Emotion-Related Beliefs And Behaviors, Deon Brown

Theses and Dissertations

This study explored African American fathers’ beliefs about and experiences with their own and their children’s negative emotions (i.e., parental meta-emotion philosophy and parental emotion socialization). Participants included 58 African American fathers in the Midwestern region of the United States between the ages of 29 and 40 (Mage = 30.94). 57 were biological fathers and one was a stepfather to toddlers between 24 and 31 months of age. Participants were invited to complete lab tasks, including the meta-emotion interview (MEI). The MEI is semi-structured interview of parents’ and their children’s negative emotion (i.e., sadness and anger). I selected a subset …

Using Individual Determinants To Predict Behavioral Health Service Use In Integrated Pediatric Primary Care, Alfonso L. Floyd Jr.

Theses and Dissertations

Mental health problems (e.g., attention, externalizing, internalizing) are commonly observed in youth and are often associated with significant impairment and an increased need for mental health services. Conceptual frameworks, such as Andersen’s Behavioral Model of Health Service Utilization, have proposed that several individual determinants (e.g., predisposing, enabling, and need factors) may influence service use for mental health problems among youth. Despite the glaring need for mental health treatment among youth with mental health problems, studies indicate that many affected youths do not receive mental health care. Integrated pediatric primary care (IPPC) settings provide an ideal setting for youth and families …

Towards The Systematic Evaluation Of Variable Modes Of Mechanical Conditioning On The Compositional, Microstructural And Mechanical Properties Of Engineered Tissue Vascular Grafts., Sarah Saunders

Theses and Dissertations

Coronary artery bypass surgery (CABG) remains one of the most common cardiac surgical procedures performed worldwide, frequently involving multiple bypasses, and commonly employing the patient’s internal mammary artery, radial artery, or saphenous vein. CABG is often not possible because native vessels were already employed in previous interventions or are diseased themselves. Synthetic vascular grafts are currently integral tools of vascular surgery and have had relative success in large-caliber applications providing substantial benefit to aortic or iliac grafting; however, small diameter (< 6 mm) arterial grafts have not yet translated into clinical effectiveness due to thrombosis and anastomotic intimal hyperplasia. ETVGs present an exciting potential alternative in vascular grafting by offering a blood vessel substitute that could exhibit all the functional characteristics of native vasculature. In addition to relieving supply limitations associated with coronary artery bypass surgery ETVGs are especially ideal for pediatric patients with congenital heart disease who require grafts that grow as they do, eliminating the need for reoccurring invasive surgeries.

Though the role of biomechanics in regulating cellular behavior promoting non-thrombogenicity, vasoactivity, and ECM synthesis and maintenance is well …

Crosstalk Between Hippo And Rb Tumour Suppressor Pathways In Ovarian Cancer, Fatmata Sesay

Theses and Dissertations

The cell cycle is a highly regulated process that ensures the timely and accurate division of cells. Events of the normal cell cycle fall under two categories - positive and negative regulatory mechanisms. The first category, positive regulatory machinery, includes active protein complexes of cyclins in association with their partnering cyclin-dependent kinases (cyclin/CDK), which mediate series of phosphorylation events that relay a cell cycle progression from one stage to the next. The second category, the negative regulatory mechanisms, include the checkpoint controls consisting of the retinoblastoma (RB) family of proteins, some of which can form a transcriptional repressor complex DREAM. …

Branched Chain Amino Acid Strain State Monitoring With Raman Spectroscopy And Plasmonic Bowtie Nanoantenna Devices For Early Disease Detection, Caroline A. Campbell

Theses and Dissertations

This work centers on the development and the down-selection of nano-manufactured devices to be used in conjunction with Raman spectroscopy for probing a branched chain amino acid. The nano-manufactured devices integrate plasmonic nanoantennas for the purpose of amplifying molecular fingerprints, which are otherwise difficult to detect, through Surface Enhanced Raman Spectroscopy (SERS). Plasmonic nanostructures can be utilized for a variety of biomedical and biochemical applications to detect the characteristic fingerprint provided by Raman Spectroscopy. The nano-manufactured devices create an electric field that amplifies minute perturbations and raises the signal above background noise. This may provide a deeper understanding of signal …

Effect Of Viscoelasticity On Cellular Morphology And Activity, Thomas J. Petet Jr

Theses and Dissertations

It has been well established that there is a link between substrate stiffness and cellular activities such as proliferation, migration, and differentiation. Less characterized is the link between the time-dependent viscosity of a substrate with those cellular activities. To explore this, PDMS substrates were created with predictably tunable stiffness and viscosity parameters. A simulated model was also developed in parallel to explore the potential effects of viscosity in a computationally predictive way. It was found that the inclusion of viscosity caused a major paradigm shift to a non-zero substrate equilibrium that was sensitive to increases in the substrate stiffness. Finally, …

Structure-Based Drug Discovery And Development Of Protein Structure Prediction Tools Using An Empirical Force Field, Noah B. Herrington

Theses and Dissertations

Traditional drug discovery has rapidly accelerated thanks to development of computational molecular modeling. The crucial component that these computational studies hinge upon is having a well-defined, and energetically favorable structure. Structures of proteins and ligands that meet these criteria are important for accurately simulating models used to study drug binding. To demonstrate the role of accurate structure simulation in the study of these events, this thesis presents, first, a story examining the problem of accurate structure modeling of ionizable residues within protein structures, specifically aspartic acid, glutamic acid, and histidine. I present our method, which uses the HINT force field …

Predicting Medication Adherence Patterns During The Maintenance Phase Of Treatment For Pediatric Leukemia And Lymphoma: A Model Of Individual- And Family-Level Factors, Stephanie M. Romo

Theses and Dissertations

Adherence during the maintenance phase of pediatric cancer treatment is critical to prevent relapse and ensure long-term, event-free survival. Yet, little research has been done to examine individual- and family-level factors that may relate to adherence during the maintenance phase of treatment, particularly among Latinx patients. This is surprising given findings demonstrating that children who miss more than 5% of their prescribed oral chemotherapy medicine, most commonly 6-mercaptopurine (6MP), are 2.5-2.7 times more likely to relapse than children who take 95% or more of their prescribed 6MP. Pediatric cancer patients face unique adherence challenges given the importance of family involvement …

Self- And Socially-Regulated Learning In Middle School Science Classrooms: A Multiple Case Study, Lauren Cabrera

Theses and Dissertations

Students must employ self-regulated learning (SRL) and socially-regulated learning (soRL) in the science classroom, which includes a wide array of independent and collaborative learning activities. However, little is known about how student SRL and soRL co-occur in students’ learning and how the classroom teacher influences that regulation in situ (Cabrera et al., in preparation; Panadero et al., 2015). This explanatory, sequential case study analyzes classroom video data from six middle school science classrooms. The study uses an integrated coding scheme that captures SRL and soRL behaviors, soRL modes, and targets of regulation (Greene & Azevedo, 2009; Hadwin et al., 2018; …

Protein Structure And Interaction: The Role Of Aromatic Residues In Protein Structure And Interactions Between Pyridoxine 5'-Phosphate Oxidase/Dopa Decarboxylase, Mohammed H. Al Mughram

Theses and Dissertations

Naturally developed proteins are capable of carrying out a wide variety of molecular functions due to their highly precise three-dimensional structures, which are determined by their genetically encoded sequences of amino acids. A thorough knowledge of protein structures and interactions at the atomic level will enable researchers to get a deep foundational understanding of the molecular interactions and enzymatic processes required for cells, resulting in more effective therapeutic interventions. This dissertation intends to use structural knowledge from solved protein structures for two distinct objectives.

In the first project, we conducted a bioinformatics structural analysis of experimental protein structures using our …

Perceived Discrimination And Mental Health Outcomes In College Students: The Mediating Role Of Preventive Health Behaviors And Social Support, Sarah C M Morton

Theses and Dissertations

Perceived discrimination has been linked to adverse mental health outcomes, increased risk-taking behaviors, and poor engagement in health promoting behaviors. College students may be especially susceptible to negative mental health outcomes associated with discrimination due to the unique stressors faced by young adults (e.g., prolonged transition to adulthood, onset of mental health disorders, changes in social support). The current study examined the mediating and moderating roles of health behaviors and social support on the association between perceived discrimination and mental health outcomes (e.g., anxiety, depression, suicidality) in college students. A total of 709 college students (42.8% White; 72.2% female; 30.2% …

Behavioral Screening And Chiral Bioanalysis Of Emerging Stimulant-Type Drugs In Rats, Tyson R. Baird

Theses and Dissertations

The epidemic of drug use in the United States and elsewhere in the world has resulted in tragic loss of life and substantial economic costs. Novel psychoactive substances (NPS) are one of the contributors to this problem, and the lack of information about many of these drugs compounds their risk. This dissertation proposes a strategy to use intracranial self-stimulation (ICSS) as a behavioral screening tool to assess emerging drugs of abuse for their abuse potential in order to generate a proactive threat assessment. A series of stimulant-type drugs including methcathinone, α-pyrrolidinohexanophenone (α-PHP), cocaine, and the phenyltropane analogs of cocaine WIN35428 …

Sleepiness: Clinical Correlates, Association With Performance, And Effects Of Performance Feedback, Spencer Nielson

Theses and Dissertations

Background: Daytime sleepiness is a common and debilitating phenomenon. Sleepiness is associated with increased symptoms of insomnia, depression, anxiety, and fatigue. Daytime sleepiness is commonly associated with cognitive performance, including performance on the Psychomotor Vigilance Test (PVT). The PVT includes performance feedback, and that feedback may influence an individual’s report of their sleepiness. Whether the feedback on the PVT influences an individual's self-reported sleepiness is unknown. This study sought to investigate how sleepiness was associated with clinical symptoms and performance, whether there are sex differences in sleepiness, and whether manipulating feedback on the PVT influenced an individual’s self-reported sleepiness. …

Identifying The Human Homologs Of Yeast Rab Proteins Ypt10 & Ypt11 And A Global-Scale Louse Endosymbiont Genome Variation, Nathaniel P. Smith

Theses and Dissertations

Amyotrophic lateral sclerosis (ALS) is a late-onset fatal neurodegenerative disease that causes loss of upper and/or lower motor neurons, and currently has no treatment or cure available. Over 90% of cases occur spontaneously with unknown causes, highlighting the complexity of the disease, and only 10% of cases are linked to heritable genetic mutations. Numerous ALS-linked genes are conserved through evolution, and model organisms may therefore provide opportunities to understand disease pathology at a molecular or cellular level, proving instrumental in identifying therapeutic targets. ALS subtype 8 (ALS8) is caused by an autosomal dominant P56S mutation in the VAPB gene that …

Examination Of Common And Rare Variant Genetic Architecture Of Psychiatric Disorders, Mohammad Ahangari

Theses and Dissertations

Psychiatric disorders are often heterogenous in their manifestation and genome-wide association studies have identified many common risk variants involved in their polygenic architectures with varying degrees of pleiotropy. In recent years, large-scale biobanks have also begun sequencing the genome of their participants to elucidate the role of rare risk variation in the genetic architecture of complex phenotypes, including psychiatric traits. This dissertation sought to better understand the role of both common and rare risk variation in the genetic architecture of psychiatric disorders with a particular focus on schizophrenia and alcohol problems. In the first three analyses, we focused on characterizing …

Smart City Management Using Machine Learning Techniques, Mostafa Zaman

Theses and Dissertations

In response to the growing urban population, "smart cities" are designed to improve people's quality of life by implementing cutting-edge technologies. The concept of a "smart city" refers to an effort to enhance a city's residents' economic and environmental well-being via implementing a centralized management system. With the use of sensors and actuators, smart cities can collect massive amounts of data, which can improve people's quality of life and design cities' services. Although smart cities contain vast amounts of data, only a percentage is used due to the noise and variety of the data sources. Information and communication technology (ICT) …

Role Of Inhibition And Spiking Variability In Ortho- And Retronasal Olfactory Processing, Michelle F. Craft

Theses and Dissertations

Odor perception is the impetus for important animal behaviors, most pertinently for feeding, but also for mating and communication. There are two predominate modes of odor processing: odors pass through the front of nose (ortho) while inhaling and sniffing, or through the rear (retro) during exhalation and while eating and drinking. Despite the importance of olfaction for an animal’s well-being and specifically that ortho and retro naturally occur, it is unknown whether the modality (ortho versus retro) is transmitted to cortical brain regions, which could significantly instruct how odors are processed. Prior imaging studies show different …

Computational Analysis Of Drug Targets And Prediction Of Protein-Compound Interactions, Sina Ghadermarzi

Theses and Dissertations

Computational prediction of compound-protein interactions generated a substantial amount of interest in the recent years owing to the importance of the knowledge of these interaction for drug discovery and drug repurposing efforts. Research suggests that the currently known drug targets constitute only a fraction of a complete set of drug targets, limiting our ability to identify suitable targets to develop new drugs or to repurpose current drugs for new diseases. These efforts are further thwarted by our limited knowledge of protein-drug (and more generally protein-compound) interactions, where only a subset of drug targets is typically known for the currently used …

Asymmetric Cuh-Catalyzed Reductive Coupling Of Alleneamides With Carbonyl Electrophiles And The Mechanistic Investigation Into The Suzuki-Miyaura Cross-Couplings Of Electron-Deficient Systems, Samantha L. Gargaro

Theses and Dissertations

Most natural products and other biologically active small molecules contain multiple stereogenic heteroatoms throughout their carbon scaffold. As a result, methods to install these multi-heteroatom functionalities efficiently are highly desirable. Reductive coupling reactions have been studied extensively, and reductive allylation has been a key method for generating chiral secondary and tertiary allylic alcohols. This work focuses on utilizing naturally abundant and inexpensive Cu for the asymmetric reductive coupling of alleneamides with carbonyl electrophiles to access highly functionalized multi-heteroatom scaffolds that are difficult to produce via traditional methods. Described herein are methods for these asymmetric reductive coupling reactions. Chapter 1 describes …

Family History Of Substance Problems Among African Americans: Associations With Drug Use, Drug Use Disorder, And Prescription Drug Misuse, Rebecca Mahrs-Gould

Theses and Dissertations

Background: Understanding individual risk for substance use and substance use disorders is important in the efforts of reducing risk and prevention of these problems. A family history of alcohol and/or drug problems often increases the risk for these problems, however the majority of research in this area is conducted in samples that are predominantly White. African Americans face disparate outcomes from substance use and substance use disorders, and the risks for substance problems may not be the same as for Whites. A systematic review of the literature identified nine studies that examined a family history of either substance problems or …

Black Hearts Matter: A Comparative Study Of Sociodemographic, Clinical, And Psychosocial Characteristics In Black And White Persons With Heart Failure, Hannah M Anderson Hughes

Theses and Dissertations

Heart failure (HF) is a chronic, progressive, debilitating illness that disproportionately affects Black individuals, often leading to poor quality of life (QOL). Various sociodemographic, clinical, and psychological characteristics are related to QOL in patients with HF. Because most studies do not include representative Black samples or report findings from racial subgroup analyses, these relationships and any existing racial differences are not well understood. The purpose of this descriptive, comparative study was to explore the relationships among sociodemographic, clinical, and psychological characteristics and QOL in patients with HF and to determine if there were racial differences between Black and White individuals. …

Examining The Impact Of Parental Racial Socialization And Critical Consciousness On Black Adolescents’ Coping With Racism-Related Stress, Chandler Alexandra Golden

Theses and Dissertations

In addition to universal stressors, Black adolescents also experience racism-related stressors. The physical and emotional consequences of racism-related stressors can be harmful to Black youth. To mitigate racism-related stress, Black youth may engage in various forms of coping. Critical consciousness and racial socialization are culturally relevant factors that have been protective against the negative impact of racism-related stress, with coping as one mechanism that undergirds this protection. Moreover, research has also begun to theorize critical action as a type of racialized coping. Past research has largely examined the impact of critical consciousness and racial socialization on coping separately and yielded …

Female Delinquency And Pathways To Crime, Pamela Lynn Smykal

Theses and Dissertations

This applied dissertation investigated gendered relationships and their potential influence on women’s criminal motivation. Historically, researchers attempted to understand criminal behavior using a male-oriented perspective which has dominated criminological literature. Criminologists have neglected to consider the role of gender as a significant predictor or pathway toward criminal behavior of either males or females. Within the last two decades, female criminality has become a spotlight for recognition and attention separate from male criminality. Traditional research on crime has been limited in understanding the pathways for female criminality and motivations for offending as individual predictors of male crime. Analyzing the role of …

The Experiences Of The Elderly Transitioning From Home To The Nursing Home Setting, Brent James Munson

Theses and Dissertations

Elderly persons transitioning from home to a nursing home setting may struggle with unaddressed mental health issues. The problem addressed in this study involved a lack of evidence regarding how elderly individuals experience these transitions in living from their own perspectives. The purpose of this study was to better understand the lived experiences of elderly people who are making the transition from home to a nursing home setting.

The qualitative design utilized in the research was phenomenology. Fourteen participants were interviewed to ascertain their experiences in the transition from home to a nursing home setting. Follow-up questions were formulated and …

A Comparative Evaluation Of The Effects Of Social Skills Group Training And Computer- Based Social Skills Training, Indira Hightower

Theses and Dissertations

This applied dissertation was designed to evaluate the effects of social skills training programs as a method to improve social skills in children diagnosed with autism spectrum disorder. The study sought to advance the social skills training options for children with autism spectrum disorder by comparing various evidence-based social skills training programs in hope to provide meaningful, significant, and sustainable social-emotional changes that are explicitly taught and later generalized across individuals, settings, and environments. The researcher specifically evaluated the effects of social skills group training and computer-based social skills training among the three study participants with autism spectrum disorder.

The …

Teacher Perceptions Of Classroom Management Programs To Improve Whole-School Measures Of Attendance, Disruptive Classroom Behaviors, And Academic Outcomes, Charles E. Natt, Jr

Theses and Dissertations

This qualitative case study explored how the implementation of Capturing Kids’ Hearts (CKH) and Multi-Tiered System of Supports (MTSS) classroom management programs when implemented together affected students and teachers of a Louisiana K-12 school compared with using the Positive Behavior Interventions and Supports (PBIS) program alone. Teachers at the school who had used the PBIS alone and then also used the newly implanted CKH and MTSS programs participated in in-depth interviews to examine the practical effectiveness of both program implementations upon students and classroom learning effectiveness. Teacher perceptions were also compared with ex post facto data for the school from …

Optimization Of In-Vitro Tissue Culture And Transformation System For Sweet Sorghum Cultivars Using Immature Inflorescence, Logayn Abushal

Theses and Dissertations

Background: Sweet sorghum is a multi-purpose crop that can be used for human food, animal feed, and bio-fuel production. This crop is drought-tolerant and can therefore be grown in arid and semi-arid regions especially in marginal areas in Egypt. Nevertheless, production of Sweet sorghum cannot coup with the increasing demand in consumption as a food, and fuel due to a lag in scientific research regarding its genetic improvement. Therefore, genetic improvement of Sweet sorghum is innately required so that new traits of economic importance could be introduced; however, this can only be achieved through the establishment of a robust in-vitro …

Sustainable Wastewater Treatment Technology For Rural Communities, Yousra Ahmed

Theses and Dissertations

Egypt is considered the country with the largest population in the Middle East Region. It is also expected to have the most significant population increase. The main objective of the present research was to develop a sustainable and effective wastewater treatment technology based on locally available materials such as sand and fabric in the treatment process. From the results obtained from the different experimental runs, it can be concluded that the use of fabric-aided sand filters achieves significant removal efficiency, especially in terms of COD and TSS. The results indicated the technical feasibility of utilizing different fabric materials with nylon …

The Utilization Of Enzymes And Enzyme Products For Antimicrobial Effects, Brandon Edward Burdette

Theses and Dissertations

Bacterial resistance is outpacing the discovery of novel antimicrobials. Therefore, it is essential to look at antimicrobials using novel methods. MTAN is a crucial enzyme for some bacteria such as Helicobacter pylori. Transition state analog inhibitor design and fragment-based drug design are drug design approaches typically used independently, but combining these techniques yields inhibitors that bind tightly and are effective in pM concentrations. We demonstrate the efficacy of the combined techniques by investigating the inhibition of HpMTAN, which is an essential enzyme. Enzyme byproducts can also be effective for causing bacterial cell death, hydrolyzing GsdmD by caspases-1/4/5/11 results in an …