Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Theses and Dissertations

Discipline
Institution
Keyword
Publication Year
File Type

Articles 3391 - 3420 of 19641

Full-Text Articles in Entire DC Network

The Role Of Chitinase A In Mastitis-Associated Escherichia Coli Pathogenesis, Weston D. Hutchison Dec 2022

The Role Of Chitinase A In Mastitis-Associated Escherichia Coli Pathogenesis, Weston D. Hutchison

Theses and Dissertations

Bovine mastitis is a common disease among dairy cattle characterized by the inflammation of the udders and loss of milk production. Mastitis-associated Escherichia coli (MAEC) are frequent causes of the disease, but the features that distinguish them from other E. coli strains remain enigmatic. MAEC infections can range from sub-clinical to severe, acute cases that can be fatal. Historically, the severity of mastitis has been attributed to host factors but more recently, a few bacterial genes have been shown to contribute to virulence in mastitis infections. In a large-scale genomic analysis of >100 MAEC isolates the gene for Chitinase A …

Go to article

Developing A Quantitative Understanding Of U-Substitution In First-Semester Calculus, Leilani Camille Heaton Fonbuena Dec 2022

Developing A Quantitative Understanding Of U-Substitution In First-Semester Calculus, Leilani Camille Heaton Fonbuena

Theses and Dissertations

In much of calculus teaching there is an overemphasis on procedures and manipulation of symbols and insufficient emphasis on conceptual understanding of calculus topics. As such students to struggle to understand and use calculus ideas in applied settings. Research shows that learning calculus topics from a quantitative reasoning-perspective results in more powerful and flexible conceptions of calculus topics like integration. However, topics beyond introducing integrals and the Fundamental Theorem of Calculus, like u-substitution, have yet to be explored from a quantity-based perspective. In this study, I conducted a set of two clinical interviews where we discussed quantitative meanings of integrals, …

Go to article

"This Whole Journey Was Sacred": Latter-Day Saint Parents' Process In Coming To Accept A Transgender Child, Julia Campbell Bernards Dec 2022

"This Whole Journey Was Sacred": Latter-Day Saint Parents' Process In Coming To Accept A Transgender Child, Julia Campbell Bernards

Theses and Dissertations

This grounded theory methodology (GTM) study examines the process of members of the Church of Jesus Christ of Latter-day Saints in coming to accept a transgender or gender diverse (TGD) child. Data comes from interviews with 38 Latter-day Saint parents of TGD children and 130 Facebook posts from the same population. Data was analyzed using GTM in coding and theory construction. A model of Latter-day Saint parents' process in accepting a TGD child and the factors that impact that process is presented. The results indicate that coming to accept a TGD child tends to engage Latter-day Saint parents cognitively, emotionally, …

Go to article

Parental Warmth And Disciplinary Strategies In Two-Parent-Adoptive And Biological Families, Jordan Coburn Dec 2022

Parental Warmth And Disciplinary Strategies In Two-Parent-Adoptive And Biological Families, Jordan Coburn

Theses and Dissertations

Adopted children enter families with parents who on average are older, have higher income, and have more education than other family structures. Because adopted children are moving into families with more resources, research suggests that they would do just as well as, and perhaps even better than, children in biological two-parent households. However, this is not always the case. Understanding how different variables could offset any negative results of adoption is a puzzle that researchers are continually exploring. Previous research has investigated differences in investments from adoptive and biological parents through multiple theoretical lenses. I seek to add to current …

Go to article

Does Predation Environment Affect Repeated Responses To Predation Cuesâ In The Fishâ Brachyrhaphis Rhabdophora?, Madeleine S. Nate Dec 2022

Does Predation Environment Affect Repeated Responses To Predation Cuesâ In The Fishâ Brachyrhaphis Rhabdophora?, Madeleine S. Nate

Theses and Dissertations

Individual organisms face trade-offs when dealing with predation—more time spent avoiding predators often results in less time allocated to energy acquisition and reproductive-related activities. Individuals that optimize this trade-off and respond appropriately to current risk levels in their environment should be at an advantage. What is less clear is whether this tradeoff changes when individuals are repeatedly exposed to a predation threat. There may be advantages to responding consistently to every signal of threat, but it might also be advantageous to modulate individual behavior. Moreover, it is unclear how evolutionary history of a population might affect such individual responses. Our …

Go to article

Decision-Based Learning As A Tool For Teaching Statistics In A Peruvian University, Gloria Mora Barba Dec 2022

Decision-Based Learning As A Tool For Teaching Statistics In A Peruvian University, Gloria Mora Barba

Theses and Dissertations

Many students find that learning statistics is difficult, especially novice learners first taking statistical classes. Instructors also struggle to teach statistics due to many challenges, such as students' misconceptions, lack of math skills, negative attitudes, and deficient instructional methods. A new pedagogical approach called Decision-Based Learning (DBL) has been developed to help instructors and students overcome these challenges. A group of Peruvian university professors in 2018 and 2019 were trained to use DBL. However, limited studies have explored DBL instructors' and students' experiences using DBL in Peru. The aim of this qualitative research study is to deeply understand the experiences …

Go to article

Identity Work To Teach Mathematics For Social Justice, Navy B. Dixon Dec 2022

Identity Work To Teach Mathematics For Social Justice, Navy B. Dixon

Theses and Dissertations

In this thesis, mathematics educators are conceptualized as individuals with multiple, simultaneous figured worlds that inform their decisions while planning. The study explores how two mathematics educators negotiated and orchestrated their figured worlds to plan two mathematics lessons for social justice. The results highlight how numerous the figured worlds each participant surfaced through background interviews. Some of the figured worlds were elicited by the interview questions (i.e., Race, Family, Social Justice Teaching) but other figured worlds organically surfaced through the interviews (i.e., Sexuality, Community, Activism, Critical Information Consumption). A discursive analysis of a selection of these figured worlds revealed conceptualizations …

Go to article

Quantifying Shape Variation In An Antisymmetrical Trait In Xenophallus Umbratilis, Mary-Elise Johnson Nielsen Dec 2022

Quantifying Shape Variation In An Antisymmetrical Trait In Xenophallus Umbratilis, Mary-Elise Johnson Nielsen

Theses and Dissertations

Antisymmetry is a striking, yet puzzling form of biological asymmetry. The livebearing fish Xenophallus umbratilis exhibits antisymmetry in the male intromittent organ and provides a system that is well-suited for studying the nature of variation in antisymmetrical traits. Using geometric morphometrics, I test the hypothesis that because the gonopodium is critical to fitness there will not be significant differences in gonopodium shape between the two gonopodial morphs in this species. My results are consistent with this prediction, though I found that gonopodium shape did differ with gonopodium size.

Go to article

Novel Patterns For Nucleosome Positioning: From In Vitro To In Vivo, David Andrew Bates Dec 2022

Novel Patterns For Nucleosome Positioning: From In Vitro To In Vivo, David Andrew Bates

Theses and Dissertations

The fundamental unit of chromatin is the nucleosome, which consists of a core of eight proteins wrapped by DNA. This core is composed of four pairs of histone proteins: H2A, H2B, H3, and H4. The DNA wraps around the protein core ~1.7 times, facilitating compaction of DNA length in the cell. Further, the location of nucleosomes makes genomic elements encoded in the DNA, such as promoters or enhancers, accessible or inaccessible to RNA polymerase and transcription factors. Thus, where nucleosomes are located (or positioned), can play a major role in transcription or other cellular processes. Additionally, histone proteins are frequently …

Go to article

Effects Of Post-Fire Disturbance Responses Of Microstegium Vimineum On Native Hardwood Seedling Growth And Survival, Zachary A. Chandler Dec 2022

Effects Of Post-Fire Disturbance Responses Of Microstegium Vimineum On Native Hardwood Seedling Growth And Survival, Zachary A. Chandler

Theses and Dissertations

Invasions by alien understory plant species have gradually become a worldwide challenge in maintaining forest biodiversity and ecosystem productivity. Notably, Microstegium vimineum, a C4 grass native to Japan, has increased dominance in mixed deciduous hardwood forests of the southeastern United States, largely due to its shade tolerance and ability to spread through rhizomes. Microstegium vimineum also proliferates after prescribed fire, a common management tool used in the region to restore forests to desired conditions, and may compete with hardwood seedlings, hindering restoration efforts. Using two upland deciduous hardwood forest research sites in Marshall and Tate Counties, Mississippi, this study …

Go to article

Losing Longleaf: Forestry And Conservation In The Southern Coastal Plain, Fraser Livingston Dec 2022

Losing Longleaf: Forestry And Conservation In The Southern Coastal Plain, Fraser Livingston

Theses and Dissertations

From the end of Reconstruction to the beginning of World War II, no other ecological change affected as great a part of the southern landscape as the loss of the longleaf pine from the southeastern coastal plain. This dissertation examines the causes and consequences of the species’ disappearance. In the span of just decades, lumber operations and naval stores producers descended upon longleaf pine woodlands with a voracious appetite that greatly contributed to the demise of the pine. However, as this dissertation argues, exploitation by the hands of the timber and turpentine industries was not the only agent that transformed …

Go to article

Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg Dec 2022

Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg

Theses and Dissertations

The work here presents two main parts. In the first part, chapters 1 – 3 focus on dynamical systems modeling in plant immunity, whereas chapters 4 – 6 describe contributions to computational modeling and analysis of proteomics and genomics data. Chapter 1 investigates dynamical and biochemical patterns of reversibly oxidized cysteines (RevOxCys) during effector-triggered immunity (ETI) in Arabidopsis, examines the regulatory patterns associated with Arabidopsis thimet oligopeptidase 1 and 2’s (TOP1 and TOP2), roles in the RevOxCys events during ETI, and analyzes the redox phenotype of the top1top2 mutant. The second chapter investigates the peptidome dynamics during ETI …

Go to article

Empathy And Cultural Involvement As Pathways To Resilience In The Face Of Adverse Childhood Experiences Among Native American Indians, Joy Rodriguez Dec 2022

Empathy And Cultural Involvement As Pathways To Resilience In The Face Of Adverse Childhood Experiences Among Native American Indians, Joy Rodriguez

Theses and Dissertations

The purpose of this research study was to examine two specific pathways to resilience for Native American Indians (NAIs) that have experienced at least one adverse childhood experience (ACE) and are seeking behavioral health services on the Choctaw Indian reservation: empathy and cultural involvement. The study is an examination of the effects of empathy and cultural involvement on the resilience of NAIs with one or more ACEs. The researcher also investigated the type of ACEs reported among adult NAIs seeking behavioral health services on the Mississippi Band of Choctaw Indian (MBCI) Reservation. Lastly, the association between ACEs and resilience was …

Go to article

A Multi-Method Exploration Of Health Disparities And Covid-19 Incidence And Mortality In The United States, S M Asger Ali Dec 2022

A Multi-Method Exploration Of Health Disparities And Covid-19 Incidence And Mortality In The United States, S M Asger Ali

Theses and Dissertations

The 21st century experienced several health crises, especially in the form of infectious disease outbreaks such as the SARS outbreak in 2003, the H1N1 in 2009, and Ebola outbreaks in 2013. However, none has produced a worldwide socio-economic and health impact compared to the recent pandemic, known as COVID-19. As of October 4, 2022, more than 614 million cases with 6 million deaths have been reported worldwide. The United States is currently in the leading position with more than 98 million cases and 1 million deaths. The pandemic, however, did not impact the entire region similarly, and the infections …

Go to article

Investigation Of The Material Properties Of Poly Pipe Irrigation Tubing For Identifying Performance Characteristics By Thickness, Victor Frank Carey Iv Dec 2022

Investigation Of The Material Properties Of Poly Pipe Irrigation Tubing For Identifying Performance Characteristics By Thickness, Victor Frank Carey Iv

Theses and Dissertations

Poly Pipe Tubing is widely used in the Mississippi Delta for supplemental irrigation of crops during the growing season. Computerized Hole Selection (CHS) is a tool used to create a prescriptive hole punching map to manage flow and minimize tail-water runoff. Some producers have documented that CHS does not work on their farm. The common thought of failure in poly pipe tubing is the rupturing or splitting of the tubing. This research was based on the thought that failure occurs before the tubing is ruptured and is caused by over pressurization during an irrigation event. Static testing revealed that there …

Go to article

Towards Structured Planning And Learning At The State Fisheries Agency Scale, Caleb A. Aldridge Dec 2022

Towards Structured Planning And Learning At The State Fisheries Agency Scale, Caleb A. Aldridge

Theses and Dissertations

Inland recreational fisheries has grown philosophically and scientifically to consider economic and sociopolitical aspects (non-biological) in addition to the biological. However, integrating biological and non-biological aspects of inland fisheries has been challenging. Thus, an opportunity exists to develop approaches and tools which operationalize planning and decision-making processes which include biological and non-biological aspects of a fishery. This dissertation expands the idea that a core set of goals and objectives is shared among and within inland fisheries agencies; that many routine operations of inland fisheries managers can be regimented or standardized; and the novel concept that current information and operations can …

Go to article

The Emerging Value Of The Viroid Model In Understanding Plant Responses To Foreign Rnas, Junfei Ma Dec 2022

The Emerging Value Of The Viroid Model In Understanding Plant Responses To Foreign Rnas, Junfei Ma

Theses and Dissertations

RNAs play essential roles in various biological processes. Mounting evidence has demonstrated that RNA subcellular localization and intercellular trafficking govern their functions in coordinating plant growth at the organismal level. Beyond that, plants constantly encounter foreign RNAs (i.e., RNAs from pathogens including viruses and viroids). The subcellular localizations of RNAs are crucial for their function. While numerous types of RNAs (i.e., mRNAs, small RNAs, rRNAs, tRNAs, and long noncoding RNAs) have been found to traffic in a non-cell-autonomous fashion within plants, the underlying regulatory mechanism remains unclear. Viroids are single-stranded circular noncoding RNAs, which entirely rely …

Go to article

Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa Dec 2022

Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa

Theses and Dissertations

The progressive increase in the global population and the rapidly changing climate have put unprecedented pressure on crop production. Cowpea is one of the world’s most important leguminous crops, contributing to food security and environmental sustainability. However, cowpea productivity is limited due to waterlogging stress. The main objective of this study was to explore physiological and biochemical mechanisms to understand how cowpea genotypes respond to waterlogging stress. Four studies were conducted in controlled and field conditions to achieve these objectives. Study 1 characterized the waterlogging tolerance of 30 cowpea genotypes in a controlled environment using 24 morphophysiological parameters with waterlogging …

Go to article

Molecular Basis Of Viroid Rna-Templated Transcription, Shachinthaka D. Dissanayaka Mudiyanselage Dec 2022

Molecular Basis Of Viroid Rna-Templated Transcription, Shachinthaka D. Dissanayaka Mudiyanselage

Theses and Dissertations

Transcription is a fundamental process catalyzed by DNA-dependent RNA polymerases (DdRPs). Interestingly, some DdRPs can use both DNA and RNA as templates for transcription. This RNA-dependent RNA polymerase (RdRP) activity of DdRPs is used by RNA-based pathogens such as viroids and hepatitis delta virus for replication. In addition, RdRP activity of DdRPs widely occurs in various organisms to regulate gene transcription. Despite the importance of this intrinsic RdRP activity of DdRPs, associated factors and mechanisms are in their infancy stage. We employed potato spindle tuber viroid (PSTVd) as a model to study RNA-templated transcription. Here, we present evidence showing that …

Go to article

Evaluation Of Novel Litter Amendments For Use In The Commercial Broiler Industry, Jorge A. Urrutia Dec 2022

Evaluation Of Novel Litter Amendments For Use In The Commercial Broiler Industry, Jorge A. Urrutia

Theses and Dissertations

Poultry litter amendments (LA) are commonly used to maintain good litter quality; the most common ones; are acidifiers. However, concern over the lifespan of LA acidifiers (d) and corrosive nature revealed a need for alternative LA. Products of interest include biochar (BC), superabsorbent polymers (SAPs), and alum mud (AM). Experiment 1 was a screening study (n=17 treatments, no birds) conducted to determine the optimal application rate/method of BC, SAPs, and AM vs.commercial LA and a control. Most SAPs treatments measured metrics may be similar (i.e., bacteria, water activity) or better (pH) vs. commercial LA (PLT). High inclusions of novel LA …

Go to article

Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden Dec 2022

Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden

Theses and Dissertations

The nursery/greenhouse industry is innovative. There have been several reports in the popular literature of non-traditional adjuvants used in plant propagation; however, these claims have never been evaluated in a scientific setting. Five studies, conducted at the South Mississippi Branch Experiment Station in Poplarville in 2019 and 2021 determined the impact that adding honey to water-soluble auxin solutions and surfactants to foliar applied auxin solutions on physiological responses associated with adventitious root formation. Treatments in studies presented in chapters two and three included three honey sources (multiflora, local, and Manuka). Honey-infused water-soluble auxin solutions affected plant species but not in …

Go to article

Surficial Geologic Mapping Of The Starkville 7.5-Minute United States Geological Survey Quadrangle 33088d-7 In Oktibbeha County, Mississippi, Jonathan Leard Dec 2022

Surficial Geologic Mapping Of The Starkville 7.5-Minute United States Geological Survey Quadrangle 33088d-7 In Oktibbeha County, Mississippi, Jonathan Leard

Theses and Dissertations

The Starkville Quadrangle is a hotspot for geological research. The Late Cretaceous is represented by the Demopolis Formation in the northeast corner of the quadrangle, followed by the Ripley Formation, and the Prairie Bluff Formation. The K-Pg boundary is exposed in the quadrangle, and the remarkable paleontology is of global importance. The Clayton Formation is the first Paleocene unit. Where the Clayton Formation channel sands are in contact with the underlying Prairie Bluff Formation, springs occur. Springs were a rare source of water in the Black Prairie and spurred the settlement of the area over 10,000 years ago. The Paleocene …

Go to article

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers Dec 2022

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …

Go to article

Alteration Of The Viral Immune Response In The Lung Of Juvenile Rats By Cannabidiol, Kylee J. Burroughs Dec 2022

Alteration Of The Viral Immune Response In The Lung Of Juvenile Rats By Cannabidiol, Kylee J. Burroughs

Theses and Dissertations

Cannabidiol (CBD) has unverified use in the treatment of ADHD but parents are giving CBD to their children to treat hyperactivity and restlessness. CBD has suppressive effects on immune function therapeutically but the effect of pre-exposure to CBD on the response to an immune challenge is unknown. To investigate this, male and female juvenile rats were treated orally with either corn oil or 20 mg/kg or 60 mg/kg CBD daily for 5 days followed 24 hours later by intranasal challenge with a viral mimic, Resiquimod (R848). After 6 hours, lungs were collected for the analysis of gene expression of select …

Go to article

Assessing The Legacy Of Erosion And Flood Control Management Efforts On The Fish Assemblages And Physical Conditions Of Yazoo Basin Bluff Hill Streams, Nicky M. Faucheux Dec 2022

Assessing The Legacy Of Erosion And Flood Control Management Efforts On The Fish Assemblages And Physical Conditions Of Yazoo Basin Bluff Hill Streams, Nicky M. Faucheux

Theses and Dissertations

The hills of Yazoo Basin have a long history of land use modification and subsequent erosion and flood control issues. In response, federal actions were taken to address these issues beginning after the Mississippi River flood of 1927. Four major flood control reservoirs were built in 1932-1957, and instream low-drop grade control structures (GCS) were installed beginning in the 1980s. The objective of my dissertation was to ascertain the long-term effects of these efforts on stream fish assemblages and channel morphology. To assess whether the reservoirs affected upstream fish assemblages as barriers to recolonization by fluvial fishes or as source …

Go to article

The Effect Of Childhood Maltreatment On Psychotherapy Effectiveness In Adulthood: Implications For Counselors, Michael Hillerman Dec 2022

The Effect Of Childhood Maltreatment On Psychotherapy Effectiveness In Adulthood: Implications For Counselors, Michael Hillerman

Theses and Dissertations

Recent gains in understanding the effects of childhood maltreatment on the development of the brain and nervous system, combined with the revelation that nearly all psychiatric neuroimaging studies have had an unrecognized confound in childhood maltreatment, imply the possibility that psychotherapy treatment effectiveness studies have been similarly confounded by childhood maltreatment. This study examines whether treatment-seeking adults exposed to childhood maltreatment respond differently to psychotherapy than do individuals who report no history of childhood maltreatment. Response to therapy is conceptualized in this study as reduction in symptom measures pre- and post- treatment, as well as client dropout. It is hypothesized …

Go to article

Oxidized Fibrin Alginate Microbeads To Treat Vascular Calcification, Brittany Nichole Macha Dec 2022

Oxidized Fibrin Alginate Microbeads To Treat Vascular Calcification, Brittany Nichole Macha

Theses and Dissertations

Calcification is linked to a high prevalence of cardiovascular events and mortality due to arterial stiffness. Stiffening of the arteries in the case of medial calcification is due to hydroxyapatite mineral deposited in the artery thus leading to the loss of elastin. A possibility of removing this rogue mineral along the vessel walls could be the use of osteoclasts. Osteoclasts, a type of osteocyte, have the unique ability to absorb bone in the bone turnover process. It is proposed that in the future, osteoclasts be delivered to the site of mineralization through oxidized alginate-fibrin microbeads. Alginate hydrogels have proven great …

Go to article

Evaluation Of Warm Season Vegetables Using Sustainable Production Practices, Jacob Arthur Dec 2022

Evaluation Of Warm Season Vegetables Using Sustainable Production Practices, Jacob Arthur

Theses and Dissertations

Sustainable practices were evaluated for the production of two warm season vegetable crops, tomato and chile peppers. The first study investigated the plant vegetative growth, fruit production timing, yield components and fruit quality of three hybrid and three heirloom tomato cultivars grown in a high tunnel using grafted and non-grafted plants. Grafting the selected tomato cultivars with the two rootstock types did not alter total marketable yield of any cultivar, but affected overall stem diameter, fruit color, and β-carotene concentrations of tested tomato cultivars. The second study evaluated the plant growth, yield, and fruit quality of nine heirloom chile pepper …

Go to article

The Use Of 87sr/86sr Isotopic Ratios To Assess Natal Areas And Movement Of Juvenile Gulf Sturgeon Acipenser Oxyrinchus Desotoi In The Pascagoula And Pearl River Systems, Joshua Neary Dec 2022

The Use Of 87sr/86sr Isotopic Ratios To Assess Natal Areas And Movement Of Juvenile Gulf Sturgeon Acipenser Oxyrinchus Desotoi In The Pascagoula And Pearl River Systems, Joshua Neary

Theses and Dissertations

The effects of water and dietary influence, and time duration for discernment, of pectoral fin spine 87Sr/86Sr were evaluated in Atlantic Sturgeon Acipenser oxyrinchus oxyrinchus. Fish were subjected to 87Sr-enriched water (0.1 mg/L) or diet (0.25 migrogram/g or 0.5 microgram/g) for 1, 2, 3, 6, or 12 weeks. Fish were also held at 15 or 25°C to determine if temperature influenced 87Sr/86Sr. Results found fin spine 87Sr/86Sr changed in water after 1 week and diet after 12 weeks suggesting water is the primary influence. In wild Gulf Sturgeon A. o. …

Go to article

Development Of A Novel Loop-Mediated Isothermal Amplification (Lamp) Assay For The Rapid Detection Of Clostridium Perfringens, Deepa Chaudhary Dec 2022

Development Of A Novel Loop-Mediated Isothermal Amplification (Lamp) Assay For The Rapid Detection Of Clostridium Perfringens, Deepa Chaudhary

Theses and Dissertations

Clostridium perfringens (C. perfringens) causes diseases such as necrotic enteritis in poultry. Current methods for early detection of C. perfringens, including culture, and molecular techniques (PCR and qPCR), have logistical limitations in detecting pathogens on-site because they require specialized equipment and long sample preparation time. This study describes developing a highly sensitive on-site LAMP assay combined with a simple DNA extraction method to detect C. perfringens. Results showed that the LAMP assay was highly specific and sensitive with the optimal reaction conditions: 63°C for 30 mins with primer concentration of 1.2 mM FIP/BIP and 0.2 mM F3/B3. The HotSHOT method …

Go to article

First Prev Next Last