Digital Commons Network^™

Tissue Engineering Cellularized Silk-Based Ligament Analogues, Scott Sell

Theses and Dissertations

The resurgence, and eventual rise to prominence in the field of tissue engineering, that electrospinning has experienced over the last decade speaks to the simplicity and adaptability of the process. Electrospinning has been used for the fabrication of tissue engineering scaffolds intended for use in nearly every part of the human body: blood vessel, cartilage, bone, skin, nerve, connective tissue, etc. Diverse as the aforementioned tissues are in both form and function, electrospinning has found a niche in the repair of each due to its capacity to consistently create non-woven structures of fibers ranging from nano-to-micron size in diameter. These …

What Happens To Student Engagement And Understanding Of Chemistry When Cross-Curricular Activities And Assignments Are Used In An Honors Chemistry Class?, Heather L. Keller

Theses and Dissertations

The focus of this action research study was to determine if cross-curricular learning strategies could be used in a chemistry class to increase both student engagement and understanding of chemistry content. Two strategies were implemented in an honors chemistry course of sophomores and juniors. Participants were chosen on a voluntary basis, those students who chose not to participate still needed to complete the activities, however, their grades and responses were not included in the data. Participating students were required to complete a consent form and obtain parental permission. Students completed a creative writing activity and a kinesthetic activity in which …

Transcriptional, Epigenetic, And Signal Events In Antifolate Therapeutics, Alexandra Racanelli

Theses and Dissertations

A targeted approach to the development of antifolate therapies has been sought for many years. Central to the success of such development is an understanding of the molecular mechanisms dictating the sensitivity of cells to antifolates and the fundamental differences of these processes between normal and neoplastic phenotypes. This dissertation addressed transcriptional mechanisms and cell-signaling events responsible for the efficacy of antifolate therapies. Transcriptional processes and cell signaling pathways are often aberrant in neoplastic tissues, providing a potential point of distinction between a normal and neoplastic cellular state. Folylpolyglutamate synthetase (FPGS) catalyzes the formation of poly-γ-glutamate derivatives of folates and …

Differentiated Interdisciplinary Science Instruction In A Fourth Grade Inclusion Classroom, Laura Oliver

Theses and Dissertations

This action research project explored what would happen if interdisciplinary, differentiated instruction was used during a science unit on recycling in a fourth grade inclusion classroom of 20 students. The project also focused in particular on the progress of two throughout the project's implementation. Four differentiated techniques were used throughout the unit. These included a tiered writing assignment, student choice based on interests, centers, and a RAFT, which was a summative assessment that allowed for student creativity based on their own interests and readiness. Using field notes, journal entries, student grades, and student surveys the data revealed a notable difference …

Investigating The Response Of Oligodendrocyte Progenitor Cells To The Cuprizone Model Of Demyelination, David Moffatt

Theses and Dissertations

Multiple sclerosis and other myelin diseases affect the quality of life many people. In the United States alone, multiple sclerosis afflicts as many as 400,000 individuals. Myelin, which is attacked by multiple sclerosis, plays a critical role in maintaining the healthy function of the adult nervous system. There are many model systems that study myelin and its formation and loss. Our lab investigates the cuprizone model of demyelination and remyelination. The cuprizone model is commonly believed only to affect adult oligodendrocytes, which it kills. The current study investigates whether other cells in the oligodendrocyte line, such as oligodendrocyte progenitor cells, …

Novel Cinchona Alkoloid Derived Ammonium Salts As Phase-Transfer Catalysts For The Asymmetric Synthesis Of Beta-Hydroxy Alpha-Amino Acids Via Aldol Reactions And Total Synthesis Of Celogentin C., Bing Ma

Theses and Dissertations

Project I. Cinchona alkaloid-derived quaternary ammonium salts have been successfully used as phase-transfer catalysts, particularly in asymmetric alkylations. Our group applied this type of catalyst in the synthesis of β-hydroxy α-amino acids via aldol reactions and discovered that the Park-Jew catalyst afforded good yields and good enantiomeric excess of the syn diasteromers, but negligible diastereoselectivity. This project was therefore focused on the synthesis of novel cinchonidine-derived catalysts with the Park-Jew catalyst as the lead structure. The C3 position of cinchonidine nucleus was modified to achieve dimers and catalysts possessing electron-deficient alkyne and alkene moieties. Synthesized catalysts were tested in the …

Roles Of Krüppel Like Factors Klf1, Klf2, And Klf4 In Embryonic Beta-Globin Gene Expression, Yousef Alhashem

Theses and Dissertations

Krüppel like factors (KLFs) are a family of 17 proteins whose main function is gene regulation by binding to DNA elements in the promoters of various genes. KLF transcription factors recognize CACCC-elements and act as activators or repressors of the gene expression. Among the 17 family members, KLF1, KLF2, and KLF4 share high homology to each other. KLF1 is the founding member of the family and is an erythroid-specific protein. KLF2 is expressed in erythroid, endothelial, and other cells. KLF4 is expressed in endothelial, smooth muscle, and other cells. In this thesis, the functions of these KLFs were reviewed in …

Investigating The Role Of Electric Fields In Directing Schwann Cell Behavior, Nishant Magar

Theses and Dissertations

This study examines the potential of Schwann cells (SCs) to be manipulated by electric fields (EFs) in order to improve recovery from spinal cord injury (SCI). It had long been believed that the central nervous system (CNS) is incapable of regeneration, but recent studies have proven otherwise. SC transplants are known to be useful in promoting axon regeneration after SCI, but is not sufficient for functional recovery. EFs are known to exist in vivo, and have been known to drastically affect the morphology and behavior of cells in various tissues. It was the hypothesis of this study that the conditioning …

Production Of Recombinant Injectosome And Outer Membrane Proteins From Yersinia Pestis Kim5, Jeremiah N. Betz

Theses and Dissertations

Molecular biology techniques and low cost reagents have lowered the barriers to entry for development of biological arsenals by non-state and state groups. Additionally, genetic engineering of epitope targets from such pathogens as Y. pestis would annul current detection methods and therapeutic treatments. Vaccines often have short shelf lives and are of minimal utility if not used prior to exposure to BW agents. Camelidae, including camels and llamas produce unique antibodies termed Nanobodies® (Nbs) or antigen specific fragments (V_HH) which are much smaller than traditional antibodies (15 vs. ~150 kDal) yet seem to attach with the same …

Expressive Writing As A Therapeutic Process For Drug Dependent Women, Meshberg Cohen Sarah

Theses and Dissertations

Women with Substance Use Disorders (SUD) have high rates of trauma and PTSD, which is linked to greater physical and mental health problems and poorer SUD treatment outcomes. While research affirms trauma should be addressed during SUD treatment, the majority of addiction programs do not offer such services. One promising intervention is Pennebaker’s expressive writing paradigm, which includes disclosure of traumatic/stressful experiences through 20-minute writing sessions over 3-5 consecutive days. While expressive writing has been linked to improvements in mental and physical health, the intervention has not been studied in persons with SUDs. The present study was a randomized clinical …

Expressive Writing With University Students With Disabilities, Geraldine Lotze

Theses and Dissertations

Research suggests college students with high incidence disabilities experience more distress than their peers without disabilities as they adapt to college. The expressive writing paradigm developed by Pennebaker and Beall (1986) effectively reduced distress in college students and other nonclinical samples when participants wrote about emotions they experienced surrounding an upsetting event. Previous research on expressive writing has not addressed the effectiveness of the paradigm with students with disabilities. A randomized control trial study examined changes in distress and daily hassles for participants with disabilities who engaged in expressive writing compared to a control condition in which participants wrote about …

Frequency Of Tlr-2, 4, 9 And Cd14 Polymorphisms In Aggressive Periodontitis Population In African-Americans, Melanie Chou

Theses and Dissertations

Aim: The aim of this study is to determine the frequency of single nucleotide polymorphisms (SNPs) in various pattern recognition receptor (PRR) genes, including Toll like receptors (TLR) -2, -4, -9, and CD14 in chronic (CP), localized (LAP) and generalized aggressive (GAP) periodontitis and periodontally healthy (NP) patients in an African American population. Methods: A total of 205 subjects were involved in the study. The LAP group consists of 25 subjects, the GAP group 50 subjects, the CP group 73 subjects and the NP group 57subjects. Genotyping was performed in TLR2 (G2408A), TLR4 (A896G),TLR9 (T1486C) and CD14 (C260T) genes by …

An Irresistible Invitation: Enhancing Academic Publication In Rhetoric And Composition By Inviting Online Peer Commentary, Sarah L. Cutler

Theses and Dissertations

In many ways the current publishing system in rhetoric and composition, which centers on the peer-reviewed journal, undermines core values we hold for ideal scholarly communication. These values include collaboration, dialogue, participation, and public engagement. Though the current system's methods of preserving, distributing, and maintaining quality control of scholarly work contradict our values, technological developments have made possible alternative publishing models that could better uphold our values. Developing a preprint archive where scholars develop and share ideas before submitting them for publication in traditional peer-reviewed journals would bring our publishing process closer to our ideals.

The Relationship Between Early Maladaptive Schemas, Chronic And Acute Depression, And Help Seeking Behaviors, Jason Walker

Theses and Dissertations

Depression is a disorder that affects many people's lives; approximately 24% of Americans are diagnosed with some form of depression in their lifetime. In order to effectively treat depression we must first understand the disorder and how the symptoms affect people. This study aims to investigate unipolar depression in two ways through the use of a questionnaire. One is to understand the etiology of depression, specifically the role of Early Maladaptive Schemas in the etiology of chronic and acute depression. Hierarchical regressions on 88 participants indicated that EMS and anxiety significantly predict acute and chronic depression. The other aim of …

Assessing Undergraduate Student Involvement: A Closer Look At The African American Pattern, Adam M. Jordan

Theses and Dissertations

The purpose of this study was to investigate the involvement patterns of selected African American undergraduate students and explore the reasoning as to why they choose to participate in certain groups and abstain from others. Data were collected through a three part survey with 60 Likert-type items used, and through a series of interviews. The population used was a convenience sample of 178 students at Rowan University's main campus in Glassboro, NJ. Eight students of that sample were purposely selected to participate in interviews. Data analysis of the surveys showed positive relationships between students who worked with classmates outside of …

Father-Daughter Attachment And Sexual Behavior In African-American Daughters, Karen Hill-Holliday

Theses and Dissertations

Although a relationship has been found in some studies between paternal attachment and female sexual behavior, knowledge of this relationship in African Americans has been limited. The purpose of this research was to determine if there was a relationship between father-daughter attachment, parent teen sexual risk communication and early sexual activity, condom use, history of sexually transmitted infection, global/sexual self-esteem and teen pregnancy in African-American females. An anonymous consent and survey was administered online to N=113 African American college women (age 18-21) attending a southeastern university. Measurements included the Parent Attachment Questionnaire (Father), Rosenberg’s Self-Esteem, Sexual Self-Esteem Inventory (short scale), …

“Design And Synthesis Of Molecular Probes For The Study Of 5-Ht2a And H1 Receptors”, Jitesh Shah

Theses and Dissertations

The serotonin (5-HT) receptors, with seven subtypes and at least fifteen distinct members, mediate a wide range of physiological functions both in the central nervous system and in the periphery. All members of the 5-HT family except the 5-HT3 subtype belong to the family of aminergic G protein-coupled receptors (GPCRs). Over the years, various molecules have been reported which act selectively at 5-HT2 receptors. However, there are no ligands that exhibit complete selectivity for one subpopulation of 5-HT2 receptors. Insight into how drugs bind to 5-HT2 receptors could contribute significantly to the development of subtype-selective agents with enhanced therapeutic effects. …

Development Of Ppar-Γ Receptor Agonists As Therapeutic Agents For Diabetes, Ashwini Goswami

Theses and Dissertations

The peroxisome proliferator-activated receptors (PPARs) are the transcriptional regulators of glucose, lipids and cholesterol metabolisms. It has been established that PPAR-γ is the receptor for thiazolidinediones (TZDs) class of type II anti-diabetic drugs. These compounds act as agonists of PPAR-γ. They may delay the development of type II diabetes in individuals at high risk of developing the condition, and have been shown to have potentially beneficial effects on cardiovascular risk factors. PPAR-γ receptor activation by TZDs improves insulin sensitivity by promoting fatty acid uptake into adipose tissue, increasing production of adiponectin (responsible for glucose regulation and fatty acid metabolism) and …

Expression Of Chemokines And Vegfs In Hnscc, Crystal Cunningham

Theses and Dissertations

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy worldwide. The 5-year survival rate when the cancer remains as a primary tumor is 81% but when it metastasizes to distant sites, defined as a metastatic cancer, it decreases dramatically to 26%. Approaches to prevent these cancers from undergoing these metastatic changes can greatly improve the survival and outcome of these cancer victims. This current study is examining the expression profiles of chemokines and VEGFs in HNSCC. By investigation the underlying pathways involved in the expressions of chemokines and VEGFS we hope to sort out the transcriptional …

Genetic Analysis Of Ribosome Stalling And Rescue, Douglas Ray Tanner

Theses and Dissertations

In eubacteria, ribosome stalling on broken messenger RNA transcripts can lead to cell death. The trans-translation quality control mechanism rescues many of these stalled ribosomes. In this process, tmRNA enters stalled ribosomes by mimicking a transfer RNA, accepting the stalled nascent peptide. The ribosome then releases the broken mRNA and resumes translation on a coding region within tmRNA itself. Translation of tmRNA marks the nascent peptide for destruction by the addition of a short proteolysis tag and the ribosome is released at a stop codon within the tmRNA open reading frame. An intriguing aspect of trans-translation is that …

How Do Elementary School Boys Characterize Their Reading Favorites And Behaviors?, Brenda J. Koonce

Theses and Dissertations

The purpose of this study was to determine if there is a boy crisis in our schools. It also looked at what the boys are interested in and what are they reading. The study investigated the latest research on young boys struggling to read, and their comparison to young girls.

The study provided evidence of their favorite books and authors. It furnished information on the boys' computer and television time. The study showed how the boys selected books and where they located new books. Percentages were presented about their personal libraries.

Overall, the study investigated how to make classrooms and …

Heritage Braided, Akiko B. Jackson

Theses and Dissertations

The awareness of marginality, oppression, and hierarchy at an early age directly influences the creation of work specific to my identity as a woman of color. Born and raised on an island in the Pacific Ocean, I was aware of my sense of location and space relative to the world. The vast ocean separating me from the ‘mainland’ created a specific understanding of marginality, of what is “main” and what is “minor,” and how these categorical placements continued from my youth to ongoing perpetuation. The work I create has a subtle and persistent investigation into my identity as a socio-economic …

Risk Factors And Protective Factors For Depression In Early Maturing Females, Lauren Lirio

Theses and Dissertations

The purpose of this study was to examine the effect of early onset of menstruation and biological father absence on depression in females. Participants (n=46) were asked to complete a demographic questionnaire and a series of psychometric assessments. It was hypothesized that females who reached puberty earlier than their peers as well as females growing up in a one-parent household, with the biological father absent, would experience more depressive symptoms than females who reached puberty later than their peers or were being raised in two-parent households. Statistical analyses revealed early maturing females reported higher levels of depressive symptoms than on-time …

On A Saturday, Thoughts And Revelations, Maria Pithara

Theses and Dissertations

A favorite poet, a strange woman on the beach, pictures I love to look at. Back yard voyeurism, some thoughts on portraiture and why I couldn’t be a photographer. Strange rituals that make sense. Unexpected revelations. Two kinds of light. This essay presents part of the constellation of thoughts, images and ideas that have informed my most recent video installation, Saturday.

Synthesis And Evaluation Of Stimulatory Properties Of Glycolipids For Natural Killer T Cells, Xiangtian Long

Theses and Dissertations

Natural killer T cells (NKT cells) are a subset of T cells. They regulate a wide range of diseases including infection, tumor growth, and autoimmune diseases, through recognizing glycolipid antigens in the context of CD1d. An understanding of the scope of glycolipid antigens would facilitate use of this cell type in controlling immune responses. Till today, a lysosomal glycolipid, isoglobotrihexosylceramide (iGb3), is the only natural glycolipid that has been found to be recognized by both human and mouse NKT cells. To elucidate the molecular basis of this specific recognition, iGb3 variants were designed and prepared: i) replacement of the C26 …

Structure And Repetition, Jon Sutter

Theses and Dissertations

This work is the result of an exploration into the relationship of form and pattern. Its inspiration began as an experiment where certain two dimensional graphical patterns were interpreted as three dimensional elements in a lattice-like assembly. The resulting compositions are built entirely of MDF, and each piece is made from multiple copies of one or two identically shaped parts that are joined with shallow grooves. Some of the pieces resemble Asian architectural lattices as well as the scientific models of molecules in crystals. Often, the flat components seem to undulate in space or twist into impossible shapes. Although the …

Is Volunteering During Adolescence Associated With A Delay In Pregnancy Until Adulthood?, Diane Bishop

Theses and Dissertations

Purpose: The objective of this study was to determine if regular volunteering during adolescence was associated with a delay in first pregnancy until adulthood. Method: Data for this analysis were drawn from Wave III of the National Longitudinal Study of Adolescent Health (Add Health). Logistic regression was used, controlling for demographic characteristics and pregnancy intention, to estimate the independent contribution of volunteering during adolescence to a delay in first pregnancy until after age 18. Results: One-third of respondents reported regularly volunteering during adolescence. Volunteering significantly differed among those who had a teen pregnancy compared to those who delayed pregnancy until …

Patients Evaluated For Liver Transplant: Transplant List Denial And Subsequent Outcomes, Kara Elam

Theses and Dissertations

Background: The evaluation process for listing a patient on the liver transplant list is complicated and involves multiple consultations from various specialists, as well as extensive imaging and physiological studies. Although there are data on the outcomes of those listed, we know little about those that are denied listing. This research project will identify the reasons for liver transplant listing denial and predictors of death following denial for this challenging group of patients. Methods: Data from all patients (n=1,500) evaluated for a liver transplant from 1997 to 2007 by the Department of Gastroenterology, Hepatology, and Nutrition located at Virginia Commonwealth …

Correlations Of Preschool Attendance And Child Study Team Classifications, Joanellen P. Fenimore

Theses and Dissertations

The purpose of the study was to explore possible relationships between children attending preschool and the Child Study Team classifications. Specifically, this researcher hypothesized that children who did not attend any form of preschool before entering Kindergarten were classified at a higher rate than those children who did receive a preschool education. There were a total of 55 participants; there were 28 male and 27 female participants. Students from the psychology pool and at the Academic Success Center at Rowan University were given questionnaires about their preschool attendance, child study team involvement, and their background. Students at the Academic Success …

Anxiety And Birth Order: Does Birth Order Play A Role In A Child's Anxiety Level?, Tarah R. Pearson

Theses and Dissertations

The purpose of this study was to see if there is an interaction between anxiety levels and birth order. It was hypothesized that the first-born child would have a higher level of total anxiety than a non-first born child. It was also hypothesized that females would have an overall higher level of anxiety than males. The Revised Children's Manifest Anxiety Scale 2^nd Edition, which was used to measure the different subtests of anxiety, tested for specific types of anxiety: total anxiety, worrying, social anxiety, physiological anxiety, and defensiveness. It was hypothesized that females would feel a higher level of …