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A Review Of Common And Rare Genetic Variants In Schizophrenia, Jonathan Luedders Jul 2011

A Review Of Common And Rare Genetic Variants In Schizophrenia, Jonathan Luedders

Theses and Dissertations

Genetic epidemiology has shown a large role for genetic influences on schizophrenia. However, the nature of the variants involved is debated. The common disease-common variant (CDCV) hypothesis suggests that schizophrenia is caused by common alleles with small effect sizes. According to the common disease-rare variant (CDRV) hypothesis, schizophrenia is caused by rare variants with large effect sizes. In recent years, evidence has been found for both common and rare variants in schizophrenia. Several SNPs have been associated with schizophrenia through genome-wide association studies (GWAS), supporting the CDCV hypothesis. In support of the CDRV hypothesis, individuals with schizophrenia have been found …

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Opioid Addiction Treatments During Pregnancy And Their Effects On Axonal Growth And Myelination In The Developing Central Nervous System, Manisha Magar Jul 2011

Opioid Addiction Treatments During Pregnancy And Their Effects On Axonal Growth And Myelination In The Developing Central Nervous System, Manisha Magar

Theses and Dissertations

Treatment with buprenorphine represents a promising alternative for pregnant opioid addicts but there is a need to understand potential effects on nervous system development. We previously showed effects of perinatal exposure to buprenorphine on axonal caliber and myelination in 26-day-old rat corpus callosum. These changes, detected at the end of rapid brain myelination and accompanied by earlier oligodendrocyte maturation, suggested interference with mechanisms coordinating axonal growth and myelination. To better understand buprenorphine actions and to establish whether these effects extend to the spinal cord, we analyzed the corpus callosum and corticospinal tract at 16 days of age, just before the …

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A Comparative Study Of A Pathogenic Versus A Nonpathogenic Naegleria Species, Melissa Jamerson Jul 2011

A Comparative Study Of A Pathogenic Versus A Nonpathogenic Naegleria Species, Melissa Jamerson

Theses and Dissertations

Naegleria fowleri (N. fowleri) and Naegleria lovaniensis (N. lovaniensis) are closely related amebae found in the environment. N. fowleri causes Primary Amebic Meningoencephalitis (PAM), a fatal disease of the central nervous system, while N. lovaniensis is nonpathogenic. N. fowleri infection occurs when amebae enter the nasal passages, and migrate to the brain. The molecular mechanisms involved in the pathogenesis of PAM are not well-defined. Therefore, the purpose of this study was to define phenotypic characteristics that may be functionally linked to the pathogenicity associated with N. fowleri. Studies revealed that N. fowleri has a faster growth rate and is more …

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Expression Profiling And Functional Validation Of Micrornas Involved In Schizophrenia And Bipolar Disorder, Albert H. Kim Jul 2011

Expression Profiling And Functional Validation Of Micrornas Involved In Schizophrenia And Bipolar Disorder, Albert H. Kim

Theses and Dissertations

MicroRNAs (miRNAs) are a family of small non-coding RNAs that regulate gene expression at both the mRNA and protein levels. MiRNAs have been shown to affect neuronal differentiation, synaptosomal complex localization and synapse plasticity, all functions thought to be disrupted in schizophrenia. We investigated the expression of 667 miRNAs (miRBase v.13) in the prefrontal cortex of individuals with schizophrenia (SZ, N = 35) and bipolar disorder (BP, N =35) using a real-time PCR-based Taqman Low Density Array (TLDA). After extensive QC steps, 441 miRNAs were included in the final analyses. At a FDR of 10%, 22 miRNAs were identified as …

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Natural Disasters And Older Adults: The Social Construction Of Disaster Planning, Elizabeth Fugate-Whitlock Jul 2011

Natural Disasters And Older Adults: The Social Construction Of Disaster Planning, Elizabeth Fugate-Whitlock

Theses and Dissertations

Examining older adults’ experiences with and response toward hurricanes within the context of the community of residence is key to both understanding their experiences and planning for future hurricanes. Specific to this research, the objective was to understand the current social network of older adults, or who provides care for whom under what circumstances, using the social constructionist perspective. Grounded theory combined with action research was the theoretical orientation guiding the study. Sources of data included the collection of household disaster plans, semi-structured interviews with older adult residents of housing authority neighborhoods, semi-structured interviews with community planners, and observation of …

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Structural Basis For Thermal Stability Of Thermophilic Trmd Proteins, Jamar Uzzell Jul 2011

Structural Basis For Thermal Stability Of Thermophilic Trmd Proteins, Jamar Uzzell

Theses and Dissertations

Thermal stability of theG37 tRNA methyltransferase proteins from Thermotoga maritima and Aquifex aeolicus have been compared using Differential Scanning Calorimetry. It was shown that the Thermotoga protein is remarkably stable and is denatured at temperatures in excess of 100 degrees Centigrade. The Aquifex aeolicus protein was less stable, denaturing broadly at temperatures between 55oC and 100oC. In contrast, the mesophilic E. coli protein was completely denatured at 55oC. Enzymatic activity of the proteins was measured at various temperatures. Both the Thermotoga and Aquifex enzymes are active at ambient temperatures, and display a significant decrease in activity when the temperature is …

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Analysis Of The Role Of Outer Surface Protein C (Ospc) In Borrelia Burgdorferi Pathogenesis, Delacy Rhodes Jul 2011

Analysis Of The Role Of Outer Surface Protein C (Ospc) In Borrelia Burgdorferi Pathogenesis, Delacy Rhodes

Theses and Dissertations

Lyme disease is an emerging infection that is caused by the Borrelia burgdorferi sensu lato complex. These bacteria exist in nature through an enzootic life cycle involving Ixodes ticks and various reservoir hosts. One way that this bacterium adapts to the different hosts in the enzootic cycle is through the expression of outer surface protein C (OspC). OspC is a surface exposed lipoprotein encoded on circular plasmid 26 that forms homodimers on the bacterial surface and has distinct conserved and variable portions of sequence. When ospC is deleted, the spirochetes are unable to cause mammalian infection although the mechanism of …

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Enhanced Degradation Of Fats, Oils And Greases In Domestic Wastewater Sewer Networks And Grease Interception Systems Using Peat Humic Substances, Matthew Hunnemeder Jul 2011

Enhanced Degradation Of Fats, Oils And Greases In Domestic Wastewater Sewer Networks And Grease Interception Systems Using Peat Humic Substances, Matthew Hunnemeder

Theses and Dissertations

The efficacy of peat humic substances in enhancing the degradation of fats, oils and greases (FOG) was investigated under controlled laboratory conditions using bench scale well-mixed bioreactors. An experimental design was used to evaluate the effects of temperature and peat humic substance (PHS) concentration on FOG degradation in domestic wastewater for a temperature range from 10 deg.C to 30 deg.C and a PHS concentration range from 0 to 20 ppm(v). Factors and interactions significantly affecting the rate of FOG degradation were identified, and models to predict FOG degradation rates as a function of PHS concentration and temperature were developed. The …

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Helper Phage Capsid Size Redirection By Staphylococcal Pathogenicity Island Sapi1 Involves Internal Scaffolding Proteins., Priyadarshan Damle Jul 2011

Helper Phage Capsid Size Redirection By Staphylococcal Pathogenicity Island Sapi1 Involves Internal Scaffolding Proteins., Priyadarshan Damle

Theses and Dissertations

Staphylococcus aureus is one of the leading causes of nosocomial and community acquired infections. Many of the virulence factors are encoded on staphylococcal mobile genetic elements. Members of the SaPI family of S. aureus mobile elements encode superantigens and are mobilized at high frequency by specific helper bacteriophages. One remarkable feature of helper phage exploitation by SaPIs is remodeling of the normal T=7 bacteriophage capsid to produce smaller T=4 phage-like particles. These particles, composed entirely of helper phage proteins, can accommodate the smaller SaPI genome while excluding that of a complete helper phage. This study was designed to understand the …

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Modeling Particle Filtration And Caking In Fibrous Filter Media, Seyed Alireza Hosseini Jul 2011

Modeling Particle Filtration And Caking In Fibrous Filter Media, Seyed Alireza Hosseini

Theses and Dissertations

This study is aimed at developing modeling methodologies for simulating the flow of air and aerosol particles through fibrous filter media made up of micro- or nano-fibers. The study also deals with modeling particle deposition (due to Brownian diffusion, interception, and inertial impaction) and particle cake formation, on or inside fibrous filters. By computing the air flow field and the trajectory of airborne particles in 3-D virtual geometries that resemble the internal microstructure of fibrous filter media, pressure drop and collection efficiency of micro- or nano-fiber filters are simulated and compared with the available experimental studies. It was demonstrated that …

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Intracellular Signaling Contributions To Behaviors Relevant To Nicotine Addiction, Lauren Thompson Jul 2011

Intracellular Signaling Contributions To Behaviors Relevant To Nicotine Addiction, Lauren Thompson

Theses and Dissertations

Nicotine is the primary addictive substance in tobacco, and most smokers who quit will relapse within a year. Evidence shows that cigarette craving increases over time, termed “incubation.” The purpose of these studies was to see if protracted abstinence from chronic nicotine increases rat self-administration, an animal model with good face validity for human tobacco use, and if nicotine self-administration during daily exposure/after 8+ days of abstinence is regulated by extracellular signal-regulated kinase (ERK) signaling in the nucleus accumbens (NAc) shell or anterior cingulate cortex (PFC). ERK kinase inhibitor U0126 was infused in the NAc shell or PFC of Long …

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Developing A Hybrid Model To Predict Student First Year Retention And Academic Success In Stem Disciplines Using Neural Networks, Ruba Alkhasawneh Jul 2011

Developing A Hybrid Model To Predict Student First Year Retention And Academic Success In Stem Disciplines Using Neural Networks, Ruba Alkhasawneh

Theses and Dissertations

Understanding the reasoning behind the low enrollment and retention rates of Underrepresented Minority (URM) students (African Americans, Hispanic Americans, and Native Americans) in the disciplines of science, technology, engineering, and mathematics (STEM) has concerned many researchers for decades. Numerous studies have used traditional statistical methods to identify factors that affect and predict student retention. Recently, researchers have relied on using data mining techniques for modeling student retention in higher education [1]. This research has used neural networks for performance modeling in order to obtain an adequate understanding of factors related to first year academic success and retention of URM at …

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Novel Methodology For Assessing Phytoplankton Response To Pco2 Enrichment In Fresh And Saltwater, Susan B. Gifford Jul 2011

Novel Methodology For Assessing Phytoplankton Response To Pco2 Enrichment In Fresh And Saltwater, Susan B. Gifford

Theses and Dissertations

Atmospheric CO2 emissions are on the rise and are expected to reach 780 parts per million by the year 2100. Research investigating the impacts of increasing CO2 is a relatively new field and the response of phytoplankton communities is largely unknown, especially in coastal and freshwater ecosystems where no CO2 manipulation studies have completed. The present study attempts to encourage uniformity in methods utilized in CO2 perturbation studies and identifies changes in phytoplankton abundance in freshwater (James River) and coastal ocean (Atlantic, Cape Hatteras) sites. A novel bubbling method to manipulate pCO2 was compared with the classic method of acid …

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Analysis And Fingerprinting Of Glycosaminoglycans, Joseph King Jul 2011

Analysis And Fingerprinting Of Glycosaminoglycans, Joseph King

Theses and Dissertations

Heparin is a complex mixture of sulfated polysaccharides derived from animals and one of the oldest drugs in use. While an efficacious anticoagulant, heparin is beset by side effects and pharmacokinetic difficulties. Low molecular weight heparins (LMWH) are made by depolymerizing unfractionated heparin (UFH) and present improvements in these areas. However, they still retain a phenomenally high level of complexity due to their polydispersity and the introduction of non-native structural features. This makes the structural characterization LMWHs a daunting task. This work details the development of a novel capillary electrophoretic (CE) method for fingerprinting LMWHs. Since their complexity normally results …

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Role Of Tyk2 In The Development Of Beige Cells, Samantha Umali Jul 2011

Role Of Tyk2 In The Development Of Beige Cells, Samantha Umali

Theses and Dissertations

Obesity results from an excess of adipose tissue and is a major risk factor for type 2 diabetes, cardiovascular disease, and cancer. Adipose tissue exists in two main forms: white adipose tissue (WAT), which stores energy as triglycerides, and brown adipose tissue (BAT), which dissipates stored energy as heat. White adipose tissue is composed of several subcutaneous and visceral depots, each possessing distinct molecular and functional characteristics. Brown-like adipocytes can emerge in WAT depots in response to cold or beta-adrenergic stimulation. These cells have been called “beige” or “brite” (brown-in-white) cells. The reduction of obesity in mice treated with beta-adrenergic …

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Racial Differences In The Genetics Of Preeclampsia, Lori Hill Jul 2011

Racial Differences In The Genetics Of Preeclampsia, Lori Hill

Theses and Dissertations

Preeclampsia (PE), characterized by hypertension and proteinuria after 20 weeks of gestation, affects 5-8% of pregnancies worldwide. Although preeclampsia is a significant cause of maternal and perinatal mortality and morbidity, its etiology remains to be elucidated. Racial differences have been observed for preeclampsia, with U.S. Blacks having higher rates and more severe disease, compared to U.S. Whites and Hispanics. One potential source of racial differences in preeclampsia is genetic variation between populations. Genetic susceptibility to preeclampsia is well established, but the specific contributions of maternal vs. fetal genes, and how these vary among racial groups is poorly understood. This dissertation …

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Sythesis And Biological Screening Of A Series Of Novel Chemokine Receptor Ccr5 Antagonists, Soundarya Vaithianathan Jul 2011

Sythesis And Biological Screening Of A Series Of Novel Chemokine Receptor Ccr5 Antagonists, Soundarya Vaithianathan

Theses and Dissertations

The chemokine receptor CCR5 has been implicated in the pathogenesis of cancers and AIDS. A series of novel piperidine derivatives were designed, synthesized, and evaluated as CCR5 antagonists. The ability of the new ligands to inhibit the increment of intracellular calcium level stimulated by endogenous ligand CCL5 was measured in the calcium mobilization assay as an indication of its CCR5 receptor antagonism. The anti-proliferation assay was performed to measure the ability of these new compounds to inhibit the proliferation of prostate cancer cell lines, PC-3 and M12. A new lead compound has been identified which showed micromolar level of inhibition …

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Ecological Profiling Of Bird-Mosquito Interactions In Central Virginia, Anna Riggan Jul 2011

Ecological Profiling Of Bird-Mosquito Interactions In Central Virginia, Anna Riggan

Theses and Dissertations

Current methods of mosquito surveillance estimate general population abundances, but fail to represent the relationship of vector abundance to host density important to determining transmission risk of mosquito-borne pathogens (MBP). We sought to address this limitation by creating a novel mosquito trap that directly sampled mosquitoes seeking to feed on nesting birds. The primary objectives of this study were to (1) assess the efficiency of the Nest Mosquito Trap (NMT) and how this is affected by nest box size. (2) assess whether the NMT affects bird, specifically nest success in Prothonotary Warblers (Protonotaria citrea), and adult behavior. (3) compare our …

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Synthesis Of A Library Of Sulfated Small Molecules, Shrenik Mehta Jul 2011

Synthesis Of A Library Of Sulfated Small Molecules, Shrenik Mehta

Theses and Dissertations

The discovery of heparin in 1916 resulted in a huge impact on the practice of medicine. Heparin has played a major role in alleviating thrombotic disorders and has also exhibited effects on almost every major system in the human body. Over the past few decades, more and more heparin-protein interactions have come to light. It is implicated to modulate several important processes such as cell growth and differentiation, inflammatory response, viral infection mechanism etc. More interesting is the observation that these interactions are considerably specific with regard to oligosaccharide sequences which have specific spatially oriented sulfate groups modulating the responses. …

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Investigation Of Pre- And Post-Zygotic Reproductive Barriers Between Two Host-Plant Complex Races Of The Parasitic Wasp Cotesia Congregata (Say) [Hymenoptera: Braconidae], Justin Bredlau Jul 2011

Investigation Of Pre- And Post-Zygotic Reproductive Barriers Between Two Host-Plant Complex Races Of The Parasitic Wasp Cotesia Congregata (Say) [Hymenoptera: Braconidae], Justin Bredlau

Theses and Dissertations

Investigations of host-associated differentiation of parasitoids have largely focused on the degree of molecular genetic differentiation, but a true test of species status must examine the mating patterns of differentiated populations to determine if they can interbreed in the wild and produce viable offspring. We examined possible mechanisms of isolation between two genetically distinct host-plant complex races of the braconid, Cotesia congregata, originating from hosts on tobacco and catalpa. We compared male responses to female pheromones, elements of male acoustic courtship signals, and breeding success between the two races. Males responded to pheromones from both sources and male courtship signals …

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Oat Snp Marker Discovery And Mapping Based On 454 Pyrosequencing Of Genome-Reduced Avena Magna Murphy Et Terrell, Rachel Rebecca Redman Jul 2011

Oat Snp Marker Discovery And Mapping Based On 454 Pyrosequencing Of Genome-Reduced Avena Magna Murphy Et Terrell, Rachel Rebecca Redman

Theses and Dissertations

The size and complexity of the oat genomes (Avena L., x = 7) have made genetic studies, including the discovery of molecular markers, difficult. Recent attention to these species has resulted in the development of many DArT -based markers in the tetraploid A. magna Murphy et Terrill (2n = 28, CCDD genomes), along with numerous RFLP's, SSR's, DArT's, and EST-based SNPs in hexaploid A. sativa L. (2n = 42, AACCDD). Here we report the first SNP markers for tetraploid oat based on genome reduction and high-throughput pyrosequencing in two inbred lines of A. magna: A-169 (wild) and Ba …

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Design And Analysis Of A Flapping Wing Mechanism For Optimization, Ryan Brandon George Jul 2011

Design And Analysis Of A Flapping Wing Mechanism For Optimization, Ryan Brandon George

Theses and Dissertations

Furthering our understanding of the physics of flapping flight has the potential to benefit the field of micro air vehicles. Advancements in micro air vehicles can benefit applications such as surveillance, reconnaissance, and search and rescue. In this research, flapping kinematics of a ladybug was explored using a direct linear transformation. A flapping mechanism design is presented that was capable of executing ladybug or other species-specific kinematics. The mechanism was based on a differential gear design, had two wings, and could flap in harsh environments. This mechanism served as a test bed for force analysis and optimization studies. The first …

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Characterization Of Lysophosphatidic Acid Subspecies Using A Novel Hplc Esi-Ms/Ms Method, Eric Mayton Jul 2011

Characterization Of Lysophosphatidic Acid Subspecies Using A Novel Hplc Esi-Ms/Ms Method, Eric Mayton

Theses and Dissertations

Lysophosphatidic acid (LPA) is a bioactive lipid with a plethora of biological functions, including roles in cell survival, proliferation, and migration. Although high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC ESI-MS/MS) technology has been used to measure the levels of LPA in human blood, serum and plasma, current methods cannot readily detect the minute levels of LPA from cell culture. In this study, a novel HPLC ESI-MS/MS method with enhanced sensitivity was developed which allows accurate measurements of LPA levels with a limit of quantitation at approximately 10 femtomoles. The method was validated by quantitation of LPA levels in …

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Resolving Incidents Of Bullying Among High Functioning Adolescents With Asd: Parent Perspectives, Wan-Ting Chuang Jul 2011

Resolving Incidents Of Bullying Among High Functioning Adolescents With Asd: Parent Perspectives, Wan-Ting Chuang

Theses and Dissertations

A qualitative study was conducted investigating the perceptions of seven parents of high functioning adolescents with autism spectrum disorders (ASD) regarding incidents of bullying in which the child was involved. Results of the study indicated that all of the children had been bullied at one time or another, and that the majority had difficulty in demonstrating appropriate social skills. The parents viewed supportive peers to be a positive factor, fostering the children's satisfactory social adjustment. Parent interventions for their child included social skills instruction and empathy training. Research implications for educators included the need to establish systemic organized school-wide support …

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Using A Multi-Sensory Teaching Approach To Impact Learning And Community In A Second Grade Classroom, Melissa Stoffers Jul 2011

Using A Multi-Sensory Teaching Approach To Impact Learning And Community In A Second Grade Classroom, Melissa Stoffers

Theses and Dissertations

The purpose of this study was to examine how multi-sensory education impacts learning and community in a second grade general education classroom. Lesson plans that targeted multiple senses - and oftentimes included team-building activities - were developed and implemented in the classroom. Qualitative data was collected through interviews, focus groups, student work, and a teacher research journal. After analyzing the data, it was revealed that multi-sensory education can lead to student motivation and engagement, as well as provide a generative space for the use of technology and making connections to the outside world. Multi-sensory education can also help teachers to …

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Mapping And Modeling Chlorophyll-A Concentrations In Utah Lake Using Landsat 7 Etm+ Imagery, Victor Nii Afum Narteh Jul 2011

Mapping And Modeling Chlorophyll-A Concentrations In Utah Lake Using Landsat 7 Etm+ Imagery, Victor Nii Afum Narteh

Theses and Dissertations

This study shows the results of testing previous research that used remote sensing techniques to determine chlorophyll-a concentrations in turbid surface waters, and developing similar methods and models for Utah Lake using Landsat 7 ETM+ satellite imagery and field measured concentrations of chlorophyll-a. The data for the study included images acquired on June 22 and July 8, 2009. The field data included ground measurements taken on June 22 and July 6, 2009 from seven water quality sampling locations. The 48 hour time difference between the Landsat image acquisition (July 8) and the field measurement (July 6), and the small sample …

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Naive Bayesian Spam Filters For Log File Analysis, Russel William Havens Jul 2011

Naive Bayesian Spam Filters For Log File Analysis, Russel William Havens

Theses and Dissertations

As computer system usage grows in our world, system administrators need better visibility into the workings of computer systems, especially when those systems have problems or go down. Most system components, from hardware, through OS, to application server and application, write log files of some sort, be it system-standardized logs such syslog or application specific logs. These logs very often contain valuable clues to the nature of system problems and outages, but their verbosity can make them difficult to utilize. Statistical data mining methods could help in filtering and classifying log entries, but these tools are often out of the …

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The Effect Of Postpartum Depression On Child Cognitive Development, Joshua Lees Jul 2011

The Effect Of Postpartum Depression On Child Cognitive Development, Joshua Lees

Theses and Dissertations

The incidence of postpartum depression (PPD) in Western societies is approximately 10-15% and its cause is multi-faceted. Because mothers largely constitute infants' social environment and mediate their experience of the external world, it is imperative to investigate the effects of PPD on child growth and development. Within this paper, the author explores previously conducted Post-Partum Depression research, in regards to its effects on child cognitive development. Following this literature review, a brief experimental study is conducted and explained on a sample population of mothers' of special needs children, serviced by an Early Intervention provider. During this experimental analysis, the researcher …

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Calcineurin Is Required For Trpv1-Induced Ltd Of Ca1 Stratum Radiatum Interneurons, Tyron Deray Jensen Jul 2011

Calcineurin Is Required For Trpv1-Induced Ltd Of Ca1 Stratum Radiatum Interneurons, Tyron Deray Jensen

Theses and Dissertations

Learning and memory in the brain are thought to be dependent on synaptic plasticity. In response to sensory input, synapses can be strengthened or weakened, known as long-term potentiation or long-term depression (LTD), respectively. Transient receptor potential vanilloid 1 (TRPV1) has been shown to mediate a novel form of presynaptic LTD in hippocampal interneurons. TRPV1 is currently being heavily studied in the PNS and being targeted by pharmaceuticals for its anti-nociceptive and anti-inflammatory properties. However, much less is known regarding TRPV1 function in the CNS, including the signal mechanism mediating hippocampal LTD despite its obvious importance. Here we performed whole-cell …

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An Invasive Species Reduces Aquatic Insect Flux To Terrestrial Food Webs, Steven S. Merkley Jul 2011

An Invasive Species Reduces Aquatic Insect Flux To Terrestrial Food Webs, Steven S. Merkley

Theses and Dissertations

Although it is well documented how introduced species can negatively affect native species, we only poorly understand how they may alter ecosystem functions. We investigated how an invasive fish affected the flux of aquatic insects to terrestrial food webs using mesocosms in a desert spring ecosystem. We compared aquatic insect emergence between alternative community states with monocultures and polycultures of two native species of fish, least chub (Iotichthys phlegethontis) and Utah chub (Gila atraria) plus, introduced western mosquitofish (Gambusia affinis). We tested three hypotheses: (1) aquatic insect biomass will be greater than terrestrial insect …

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