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A Water Quality Investigation Of Kimages Creek, Michael Trop Aug 2012

A Water Quality Investigation Of Kimages Creek, Michael Trop

Theses and Dissertations

Analysis of continuous monitoring water quality data (temperature, specific conductivity, depth, pH, dissolved oxygen, and turbidity) at two locations in a tidal freshwater creek (Kimages Creek) characterized seasonal variation and responses to short-term events. Supplemental water quality measurements were collected to describe longitudinal variations in the creek. There were significant differences in water quality between the two continuous monitoring stations (one tidal and unforested, the other non-tidal and forested) over varying time scales. Rain events showed increases in turbidity, depth and dissolved oxygen, and reductions in temperature, conductivity, and pH at both stations. Tides influenced the water quality at the …

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Combinatorial Analysis Of Tumorigenic Micrornas Driving Prostate Cancer, William Budd Aug 2012

Combinatorial Analysis Of Tumorigenic Micrornas Driving Prostate Cancer, William Budd

Theses and Dissertations

Prostate cancer is the leading non-cutaneous malignancy affecting men in the United States. One in every six men will be affected by prostate cancer. Due to the high incidence of prostate cancer, there is a need to develop biomarkers capable of identifying tumors from benign prostatic lesions. miRNAs are small molecules that regulate protein translation and impact cellular integrity when dysregulated. It is widely thought that miRNAs have the potential to serve as biomarkers. This study utilizes a unique combinatorial analysis of miRNA dysregulation to identify key miRNAs involved in prostate tumor initiation, progression and metastasis. Numerous dysregulated miRNAs potentially …

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Integrated Fiber Electrospinning: Creating Spatially Complex Electrospun Scaffolds With Minimal Delamination, Casey Grey Aug 2012

Integrated Fiber Electrospinning: Creating Spatially Complex Electrospun Scaffolds With Minimal Delamination, Casey Grey

Theses and Dissertations

Tissue engineering scaffolds come in many shapes and sizes, however, due to difficulty manufacturing the microstructure architecture required in tissue engineering, most scaffolds are architecturally non-dynamic in nature. Because the microstructural architecture of all biological tissues is inherently complicated, non-dynamic tissue engineering scaffolds tend to be a poor platform for tissue regeneration. The current method for manufacturing dynamic tissue engineering scaffolds involves electrospinning successive layers of different fibers, an approach that exhibits no fiber transition between layers and subsequent delamination problems. In this study we aim to address the design challenges of tissue engineering scaffolds through our novel integrated fiber …

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The Longitudinal Stability Of Memory In Males With Autism Spectrum Disorder, Alexander J. Cramond Aug 2012

The Longitudinal Stability Of Memory In Males With Autism Spectrum Disorder, Alexander J. Cramond

Theses and Dissertations

Previous research has demonstrated mixed evidence on impaired memory functioning in autism spectrum disorder (ASD), with the only consensus that there appears to be much heterogeneity. In addition, no research to date has examined the stability of memory in ASD. This study examined the stability of memory function in ASD compared to typically developing age-matched controls. Participants were administered the Test of Memory and Learning (TOMAL) twice, three years apart, in an established longitudinal NIH-supported investigation of ASD. Based on available research contrasting memory development in healthy individuals versus those with ASD, it was hypothesized that memory performance in the …

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Ocean Acidification: Understanding The Coastal Carbon Pump In A High Co2 World, Rachel Cooper Aug 2012

Ocean Acidification: Understanding The Coastal Carbon Pump In A High Co2 World, Rachel Cooper

Theses and Dissertations

Since the 1800s, carbon dioxide emissions due to human activities have contributed significantly to the amount of carbon in the atmosphere. Approximately a third of this carbon is absorbed by the ocean, through air-sea fluxes at the ocean surface (Sabine, 2004). Increased CO2 has changed the carbon chemistry of the ocean and hence the pH. pH is expected to drop by 0.4 by the year 2100. It is unclear how this lower pH will affect carbon cycling and sequestration with respect to the biological carbon pump. Most studies have focused on open ocean phytoplankton or bacterial communities in large, stationary …

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Suspended Polypyrrole Films Supporting Alamethicin Reconstituted Bilayer Membranes, Robert Northcutt Aug 2012

Suspended Polypyrrole Films Supporting Alamethicin Reconstituted Bilayer Membranes, Robert Northcutt

Theses and Dissertations

This thesis presents a novel architecture for a sensing element fabricated from a conducting polymer and a bioderived membrane. The thin film device provides controlled, selective ion transport from a chemical concentration and produces measurable electrical signals, ion storage, and small scale actuation. A chemical gradient applied across a bioderived membrane generates ion flow through protein transporters in the presence of a gating signal. A conducting polymer undergoes ion ingress/egress in the presence of an electrical and chemical potential, which causes a change on the polymers conformal backbone. A ligand (or) voltage gated protein in the bioderived membrane results in …

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Essential Spawning Habitat For Atlantic Sturgeon In The James River, Virginia., Geoffrey Austin Aug 2012

Essential Spawning Habitat For Atlantic Sturgeon In The James River, Virginia., Geoffrey Austin

Theses and Dissertations

Substrate composition plays a critical role in determining the spawning success of Atlantic sturgeon. A benthic analysis of the tidal freshwater portion of the James River, Virginia, was performed to locate and protect remaining sturgeon spawning habitat within the James River system. I modeled structural habitat, substrate distribution, and river bathymetry from Richmond, Virginia to the Appomattox River confluence. A classification model was developed to describe the dominant substrate type (mud/silt, sand, gravel, bedrock) using side scan sonar data collected from August 2011-Febuary 2012. River depth, bottom imagery, substrate density (hardness), and ground truth substrate samples were interpolated into a …

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Sorafenib Enhances Pemetrexed-Induced Cytotoxicity Through And Autophagy-Dependent Mechanism In Cancer Cells, Bareford Mary Aug 2012

Sorafenib Enhances Pemetrexed-Induced Cytotoxicity Through And Autophagy-Dependent Mechanism In Cancer Cells, Bareford Mary

Theses and Dissertations

Acquired cellular resistance to traditional chemotherapeutics is a common obstacle in the treatment of most cancer cell types. This resistance occurs as a result of changes in the underlying molecular mechanisms of disease progression. The development of novel chemotherapeutic approaches designed to enhance the efficacy of protypical anti-cancer drugs is important in order to overcome this issue. Such approaches will aid in understanding the biomolecular phenomena responsible for drug resistance and disease progression. Combining signaling pathway inhibitors has become an effective strategy for enhancing tumor cell death by targeting multiple pathways known to regulate cell survival. Pemetrexed, an FDA-approved anti-folate …

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Preparation And Characterization Of A Self-Crimp Side-By-Side Bicomponent Electrospun Material, Yang Han Aug 2012

Preparation And Characterization Of A Self-Crimp Side-By-Side Bicomponent Electrospun Material, Yang Han

Theses and Dissertations

Bicomponent composite fibers have been widely used in the textile industry and are gaining increasing attention on biomedical applications. In this research, polycaprolactone/poly (lactic acid) side-by-side bicomponent fibers were created for the application of a biodegradable scaffold. The side-by-side structure endowed the fiber with self-crimps when it was processed under certain conditions. This material was produced by electrospinning and collected on a high speed rotating mandrel to get highly oriented fibers. A mechanical stretch at the same direction was done followed by a wet heat treatment for polymer retraction. Crimped fibers were demonstrated by scanning electron microscopy. The quantitative porosity …

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Transgene Delivery Via Microelectromechanical Systems, Aubrey Marie Mueller Wilson Aug 2012

Transgene Delivery Via Microelectromechanical Systems, Aubrey Marie Mueller Wilson

Theses and Dissertations

The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene delivery option. In this work we address the current progress of microelectromechanical systems (MEMS) used as transgenic delivery mechanisms. The nanoinjector is a specially designed MEMS device which uses electrostatic charge to manipulate transgene molecules. The process of nanoinjection was designed as an alternative to microinjection which causes less damage to developing embryos, improves embryo survival, birth rates, and overall efficiency of injections. In vivo testing of nanoinjection demonstrates it is both safe and effective. …

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Assessment Of The Role Of Solute Carrier Drug Transporters In The Systemic Disposition Of Fluoroquinolones: An In Vitro - In Vivo Comparison, Aditi Mulgaonkar Aug 2012

Assessment Of The Role Of Solute Carrier Drug Transporters In The Systemic Disposition Of Fluoroquinolones: An In Vitro - In Vivo Comparison, Aditi Mulgaonkar

Theses and Dissertations

Fluoroquinolones (FQ) are broad-spectrum charged antimicrobials exhibiting excellent tissue/fluid permeation. Thus, FQ disposition depends essentially on active transport and facilitative diffusion. Although most early transporter studies investigating renal elimination of FQs have focused on apical efflux of FQs from renal proximal tubule cell (RPTC) into urine, their basolateral uptake mechanism(s) from blood into RPTC (i.e., first step to tubular secretion) has not yet been explored in detail. Renally expressed SLC22 members: organic anion (OATs) and cation (OCTs) transporters are known to transport such small organic ionic substrates (molecular weight ~400 Da). Hence it is of interest to explore the role …

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Mathematical Model And Experimental Exploration Of The Nanoinjector Lance Array, Nathan C. Toone Jul 2012

Mathematical Model And Experimental Exploration Of The Nanoinjector Lance Array, Nathan C. Toone

Theses and Dissertations

The Nanoinjector Lance Array has been developed to inject foreign material into thousands of cells at once using electrophoresis to attract and repel particles to and from the electrically-charged lances. A mathematical computer model simulating the motion of attracted or repelled proteins informs the design of the nanoinjection lance array system. The model is validated by accurately predicting protein velocity in electrophoresis experiments. A complete analysis of parameters is conducted via simulations and specific research questions regarding the counter electrode of the nanoinjector lance array system are explored using the model. A novel technique for fabricating lance arrays from collapsed …

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In Vitro In Vivo Methods And Pharmacokinetic Models For Subcutaneously Administered Peptide Drug Products, Amit Somani Jul 2012

In Vitro In Vivo Methods And Pharmacokinetic Models For Subcutaneously Administered Peptide Drug Products, Amit Somani

Theses and Dissertations

Over the last several years, injectable drugs have been a growing area for the treatment of various therapeutic conditions and they are projected to comprise an even larger proportion among the drugs that will be available in the years to come. The injectable drugs are administered by various routes such as intramuscular (IM), intravenous (IV), subcutaneous (SC) and others, however, the majority of these drugs are administered subcutaneously. Even though subcutaneous delivery has been utilized for a number of years, very little is known about the processes governing the absorption of macromolecules from the interstitial space; and the resulting impact …

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Spag16 Is A Bifunctional Gene Regulating Male Fertility, David R. Nagarkatti-Gude Jul 2012

Spag16 Is A Bifunctional Gene Regulating Male Fertility, David R. Nagarkatti-Gude

Theses and Dissertations

SPAG16 is the murine orthologue of Chlamydomonas reinhardtii PF20, a protein known to be essential to the structure and function of the “9 + 2” axoneme. The “9 + 2” axoneme provides the cytoskeletal core of all eukaryotic motile cilia and flagella. In Chlamydomonas, the Pf20 gene encodes a single protein present in the central pair of the axoneme. Loss of Pf20 prevents central pair assembly and results in flagellar paralysis. The murine Spag16 gene encodes two proteins. While 71 kDa SPAG16L is found in all murine cells with motile cilia or flagella, 35 kDa SPAG16S transcript and protein are …

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Design And Testing Of A Biological Microelectromechanical System For The Injection Of Thousands Of Cells Simultaneously, Gregory Herlin Teichert Jul 2012

Design And Testing Of A Biological Microelectromechanical System For The Injection Of Thousands Of Cells Simultaneously, Gregory Herlin Teichert

Theses and Dissertations

The ability to inject DNA and other foreign particles into cells, both germ cells (e.g. to produce transgenic animals) and somatic cells (e.g. for gene therapy), is a powerful tool in genetic research. Nanoinjection is a method of DNA delivery that combines mechanical and electrical methods. It has proven to have higher cell viability than traditional microinjection, resulting in higher integration per injected embryo. The nanoinjection process can be performed on thousands of cells simultaneously using an array of microneedles that is inserted into a monolayer of cells. This thesis describes the needle array design requirements and the fabrication process …

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Effects Of Leaf Litter Diversity On Nutrients And Mosquito Communities In Neotropical Artificial Tree Holes, Rachel Komosinski Jul 2012

Effects Of Leaf Litter Diversity On Nutrients And Mosquito Communities In Neotropical Artificial Tree Holes, Rachel Komosinski

Theses and Dissertations

Inputs from terrestrial habitats to aquatic habitats are important for structuring aquatic communities. Terrestrial producer diversity in the tropics may decline due to anthropogenic causes. I investigated how tree diversity affects aquatic communities. We used leaves from three timber-producing species (Dalbergia retusa, Pachira quinata, and Tectona grandis) to test the effects of leaf litter species composition and richness on invertebrate aquatic communities in Gamboa, Panama. We quantified macroinvertbrate species richness and abundances, leaf litter mass loss, and dissolved carbon to nitrogen ratios (C:N) after 4 weeks. We found that litter types differed in breakdown and C:N. Tectona grandis had lower …

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The Effect Of A Navigational Aid On Training Of A Minimally Invasive Surgery Camera Task, Ketan Vidwans Jul 2012

The Effect Of A Navigational Aid On Training Of A Minimally Invasive Surgery Camera Task, Ketan Vidwans

Theses and Dissertations

Minimally Invasive Surgery (MIS) differs from Open Surgery as surgeons view the surgical site indirectly on a monitor. The view shown is typically from an angled endoscope off to one side of the surgery (i.e., uncollocated with the view of the hands). This makes camera navigation a challenging ability to learn. MIS thus requires longer training periods, more practice and mental effort to achieve proficiency. Current training setups and Operating Room (OR) environments lack appropriate real-time visual cues for navigation and other perception related information that could help with learning and performance in the OR. The purpose of this research …

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Prediction Of Human Systemic, Biologically Relevant Pharmacokinetic Properties Based On Physicochemical Properties Of Calcium Channel Blockers, Tafif Abdullah Al Jul 2012

Prediction Of Human Systemic, Biologically Relevant Pharmacokinetic Properties Based On Physicochemical Properties Of Calcium Channel Blockers, Tafif Abdullah Al

Theses and Dissertations

This research explored quantitative relationships (QSPKR) between different molecular descriptors and pertinent, systemic PK properties for 14 calcium channel blockers (CCB). Physicochemical properties (PC) such as molecular weight (MW), molar volume (MV), calculated logP (clogP), pKa, calculated logD7.4 (clogD), % ionized at pH 6.3 and pH 7.4, hydrogen bond donors (HBD), hydrogen bond acceptors (HBA), and number of rotatable bonds (nRot) were chosen as possible predictor variables for systemic PK properties for CCB, obtained from pertinent literature, assessing the PK of CCB after intravenous administration to healthy humans. All PC properties and molecular descriptors were computed using ACD-solubility/DB 12.01. Total …

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Characterizing The Interaction Between Ve-Ptp, Tie2 And Ve-Cadherin, Sharif Ossai Muhammad Jul 2012

Characterizing The Interaction Between Ve-Ptp, Tie2 And Ve-Cadherin, Sharif Ossai Muhammad

Theses and Dissertations

Many signaling pathways have been shown to be involved in the formation of the vascular system. Among them are the endothelial specific receptor families such as VEGF, Ang/Tie, as well as other signaling pathways such as semaphorins, which are also involved, in axonal guidance. It is known that the interaction between receptor tyrosine kinase, Tie2, VE-Cadherin, and VE-PTP mediate endothelial cell quiescence and adhesion. However, the structural basis of these interactions is not well understood. The aim of our study is to characterize the binding interactions between these players. Another important part of our study is describing the cross-talk between …

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Using Next Generation Sequencing (Ngs) To Identify And Predict Micrornas (Mirnas) Potentially Affecting Schizophrenia And Bipolar Disorder, Vernell Williamson Jul 2012

Using Next Generation Sequencing (Ngs) To Identify And Predict Micrornas (Mirnas) Potentially Affecting Schizophrenia And Bipolar Disorder, Vernell Williamson

Theses and Dissertations

The last decade has seen considerable research focusing on understanding the factors underlying schizophrenia and bipolar disorder. A major challenge encountered in studying these disorders, however, has been the contribution of genetic, or etiological, heterogeneity to the so-called “missing heritability” [1-6]. Further, recent successes of large-scale genome-wide association studies (GWAS) have nonetheless seen only limited advancements in the delineation of the specific roles of implicated genes in disease pathophysiology. The study of microRNAs (miRNAs), given their ability to alter the transcription of hundreds of targeted genes, has the potential to expand our understanding of how certain genes relate to schizophrenia …

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A Longitudinal Study Of The Effect Of At Birth Adoptions On Anxiety, Stress Hormones And Adolescent Alcohol Intake: A Nonhuman Primate Model, Whitney Faith Maxwell Jul 2012

A Longitudinal Study Of The Effect Of At Birth Adoptions On Anxiety, Stress Hormones And Adolescent Alcohol Intake: A Nonhuman Primate Model, Whitney Faith Maxwell

Theses and Dissertations

Adopted individuals have an increased risk for a variety of psychopathological disorders. Studies of the effects adoption in humans are difficult to perform because of the difficulty separating genetic risk and treatment effects. This is a developmental study investigating the effects of at birth adoption using a nonhuman primate model. Three experimental paradigms were used to assess maternal treatment, stress-related behavior, and physiology late in infancy and again later in life. Rhesus monkeys were reared for their first six months of life by either their biological mother or an unrelated, lactating adult female. Adoptions occurred immediately following birth. At six …

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Accurate Methodology For Monitoring Biomembrane Events, Christine A. Winschel Jul 2012

Accurate Methodology For Monitoring Biomembrane Events, Christine A. Winschel

Theses and Dissertations

Abstract ACCURATE METHODOLOGY FOR MONITORING BIOMEMBRANE EVENTS By Christine A. Winschel, Ph.D. A Dissertation submitted in partial fulfillment of the requirements for the degree of Doctorate of Philosophy in Chemistry at Virginia Commonwealth University. Virginia Commonwealth University, 2012 Major Director: Dr. Vladimir A. Sidorov ASSOCIATE PROFESSOR, DEPARTMENT OF CHEMISTRY This study describes the synthesis and characterization of a new receptor (cyclen 1) capable of strong selective binding of pyrene-based anionic dyes under near-physiological conditions. This receptor comprises four naphthylthiourea groups tethered to a cyclen core via an ester linkage. The most important finding was the ability of cyclen 1 to …

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Sex-Specific Habitat Use And Responses To Fragmentation In An Endemic Chameleon Fauna, Philip Shirk Jul 2012

Sex-Specific Habitat Use And Responses To Fragmentation In An Endemic Chameleon Fauna, Philip Shirk

Theses and Dissertations

Chameleons are an understudied taxon facing many threats, including collection for the international pet trade and habitat loss and fragmentation. A recent field study reports a highly female-biased sex ratio in the Eastern Arc Endemic Usambara three horned chameleon, Trioceros deremensis, a large, sexually dimorphic species. This species is collected for the pet trade, and local collectors report males bring a higher price because only this sex has horns. Thus, sex ratios may vary due to differential rates of survival or harvesting. Alternatively, they may simply appear to be skewed if differences in habitat use biases detection of the sexes. …

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Signal Transduction Effects Induced By Erythropoietin In A Hnscc Model System, Shreya Desai Jul 2012

Signal Transduction Effects Induced By Erythropoietin In A Hnscc Model System, Shreya Desai

Theses and Dissertations

Head and Neck Squamous Cell Carcinoma (HNSCC) is an epithelial skin cancer of the upper aerodigestive tract, and is the sixth most common malignancy in the U.S. HNSCC patients undergoing chemotherapy commonly develop anemia, a condition in which the body lacks mature red blood cells (RBCs). Erythropoietin (EPO) is a systemically circulating hormone in the body that regulates the production of RBCs and is applied to treat anemia. Recently, several studies implicated shortened life expectancy of cancer patients by EPO administration. It may be due to an unexpected activation of survival and proliferation pathways of cancer cells by EPO because …

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Life History Switch Point Plasticy In Response To Pond Drying Alyers Metamorph Allometry And Jumping Performance, Julie Charbonnier Jul 2012

Life History Switch Point Plasticy In Response To Pond Drying Alyers Metamorph Allometry And Jumping Performance, Julie Charbonnier

Theses and Dissertations

Animals with complex life cycles can cope with environmental uncertainty by altering life history switch points through developmental plasticity. Pond drying is an important factor which may alter life history switch points in aquatic organisms. Many amphibians can plastically respond to changes in pond drying by emerging earlier, but few studies have examined the post-metamorphic consequences for performance. To investigate the potential carry-over effects of plasticity to pond drying, we studied the túngara frog, Physalaemus pustulosus, a tropical anuran that breeds in highly ephemeral habitats. We conducted a field study with three different water depth treatments in 60 L mesocosms …

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Role Of Fyn And Lyn In Igg-Mediate Immune Responses, Yves Falanga Jul 2012

Role Of Fyn And Lyn In Igg-Mediate Immune Responses, Yves Falanga

Theses and Dissertations

Anaphylaxis is a rapid, life-threatening hypersensitivity reaction. Until recently, it was mainly attributed to histamine released by mast cells activated by allergen crosslinking (XL) of FcεRI-bound allergen-specific IgE. However, recent reports established that anaphylaxis could also be triggered by basophil, macrophage and neutrophil secretion of platelet activating factor subsequent to FcγR stimulation by IgG/Ag complexes. I have investigated the contribution of Fyn and Lyn tyrosine kinases to FcγRIIb and FcγRIII signaling in the context of IgG-mediated passive systemic anaphylaxis (PSA). I found that mast cell IgG XL induced Fyn, Lyn, Akt, Erk, p38 and JNK phosphorylation. Additionally, IgG XL of …

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The Examination Of Inter-Rater Reliability And The Reliability Of Attachment Measures, Nicole Bayles Jul 2012

The Examination Of Inter-Rater Reliability And The Reliability Of Attachment Measures, Nicole Bayles

Theses and Dissertations

The purpose of this study was to determine the reliability of measuring attachment through subjective measures, as well as the inter-rater reliability of those using the subjective measures. Participants were volunteers who had worked with the Division of Youth and Family Services (DYFS). They included social workers, lawyers, psychologists, etc. Participants were asked to answer questionnaires regarding the DYFS children they had worked with, as well as demographics about themselves. The questionnaires also included an alternative informed consent. The surveys were mailed to participants and included return envelopes to ensure ease of mailing them back. It was hypothesized that measuring …

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Role Of Nucleosome Remodeling Factor (Nurf) In Tumorigenesis Using A Breast Cancer Mouse Model, Aiman Alhazmi Jul 2012

Role Of Nucleosome Remodeling Factor (Nurf) In Tumorigenesis Using A Breast Cancer Mouse Model, Aiman Alhazmi

Theses and Dissertations

Understanding the impact of epigenetic mechanisms on tumorigenesis is essential, as epigenetic alterations are associated with tumor initiation and progression. Because epigenetic changes are reversible, they are potential targets for cancer therapy. Nucleosome Remodeling Factor (NURF) is a chromatin-remodeling complex that regulates gene expression by changing nucleosome positioning along the DNA sequence. Previous studies have shown a role for NURF in embryonic development as well as regulating genes involved in tumor progression. In this work we investigated the impact of eliminating NURF function in tumorigenesis in vivo. BALB/c mice challenged with syngeneic 67NR breast cancer cell lines, injected into the …

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Student Autonomy: A Case Study Of Intrinsic Motivation In The Art Classroom, Downi Griner Jul 2012

Student Autonomy: A Case Study Of Intrinsic Motivation In The Art Classroom, Downi Griner

Theses and Dissertations

How might a curriculum based on Self-Determination Theory (SDT) influence student motivation and art making in a 9th grade art classroom? The researcher devised a negotiated curriculum called The Master Artist Program based on the SDT theory of intrinsic motivation. The implementation of this curriculum was designed to explore the question of how a curriculum based on SDT theory would influence student motivation and art making in a 9th grade art classroom. This curriculum was implemented in a ninth grade art class on an optional basis for the course of nine weeks. The results of data analysis, illustrated by relevant …

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Cell Death And Growth Arrest Pathways Mediating The Actions Of Stilbene 5c In Hct-116 Colon Cancer Cells, Moureq Alotaibi Jul 2012

Cell Death And Growth Arrest Pathways Mediating The Actions Of Stilbene 5c In Hct-116 Colon Cancer Cells, Moureq Alotaibi

Theses and Dissertations

Abstract The stilbene derivative, cis-3, 4’, 5-trimethoxy-3’-aminostilbene (stilbene 5c), is a potentially potent antitumor agent that acts via binding to the colchicine-binding pocket in microtubules. Earlier studies have shown that stilbene 5c induces cell death in ovarian cancer cells and leukemic cells. The present study was designed to investigate the effectiveness of this microtubule poison against the HCT-116 human colon cancer cell line and its mechanisms of action. Time course studies demonstrated that stilbene 5c produces a biphasic decrease in cell viability. The capacity of the cells to proliferate was not restored upon removal of the drug after 6 days …

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