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Partial Purification And Characterization Of Texas Live Oak (Quercus Fusiformis) Lectin, Carmen Guadalupe Cruz

Theses and Dissertations

Lectins are carbohydrate-binding proteins with agglutination properties. There is a continuous interest in lectins due to their biological properties that can be exploited for medicinal and therapeutic purposes. The objective of this study was to isolate and characterize lectin in Texas Live Oak (Quercus fusiformis). More specifically, the study aimed to determine the lectin’s blood group specificity and pH stability, determine effects of seasonal variation, soil moisture and soil pH on lectin activity. The study also aimed to determine the presence of antifungal activity in Q. fusiformis extracts. Lectin activity was detected and compared via agglutination and protein assays. Protein …

Richard Wagner's Jesus Von Nazareth, Matthew Giessel

Theses and Dissertations

In addition to his renowned musical output, Richard Wagner produced a logorrhoeic prose oeuvre, including a dramatic sketch of the last weeks of the life of Jesus Christ entitled Jesus von Nazareth. Though drafted in 1848-1849, it was published only posthumously, and has therefore been somewhat neglected in the otherwise voluminous Wagnerian literature. This thesis first examines the origins of Jesus von Nazareth amidst the climate of revolution wherein it was conceived, ascertaining its place within Wagner’s own internal development and amongst the radical thinkers who influenced it. While Ludwig Feuerbach has traditionally been seen as the most prominent of …

Metabolic Remodeling And Mitochondrial Dysfunction In Maladaptive Right Ventricular Hypertrophy Secondary To Pulmonary Arterial Hypertension, Jose Gomez-Arroyo

Theses and Dissertations

Right ventricular dysfunction is the most frequent cause of death in patients with pulmonary arterial hypertension. Although abnormal energy substrate use has been implicated in the development of chronic left heart failure, data describing such metabolic remodeling in failing right ventricular tissue remain incomplete. In the present dissertation we sought to characterize metabolic gene expression changes and mitochondrial dysfunction in functional and dysfunctional RV hypertrophy. Two different rat models of RV hypertrophy were studied. The model of right ventricular failure (SU5416/hypoxia) exhibited a significantly decreased gene expression of peroxisome proliferator-activated receptor- coactivator-1α, peroxisome proliferator- activated receptor-α and estrogen-related receptor-α. The …

Shaping This Space Between Us: Using A Reflexive Journaling Process To Explore The Complex And Malleable Space In Which Identity Exists, Lina Mahusain

Theses and Dissertations

While some parts of identity remain constant throughout one’s life, many aspects of a person’s identity are subject to rapid and constant change and negotiation. Using theories regarding the role possessions play in the construction, reinforcement, and manifestation of a person’s identity, this thesis project explores the potential to facilitate meaningful insights and awareness into one’s own identity. This will be achieved by designing a reflexive journaling process. A qualitative evaluation of this prototype journal process used by a pilot group of young creative individuals will generate an assessment of the proposed process

Hybrid Pet/Mri Nanoparticle Development And Multi-Modal Imaging, David Hoffman

Theses and Dissertations

The development of hybrid PET/MRI imaging systems needs to be paralleled with the development of a hybrid intrinsic PET/MRI probes. The aim of this work was to develop and validate a novel radio-superparamagnetic nanoparticle (r-SPNP) for hybrid PET/MRI imaging. This was achieved with the synthesis of superparamagnetic iron oxide nanoparticles (SPIONs) that intrinsically incorporated 59Fe and manganese iron oxide nanoparticles (MIONs) that intrinsically incorporated 52Mn. Both [59Fe]-SPIONs and [52Mn]-MIONs were produced through thermal decomposition synthesis. The physiochemical characteristics of the r-SPNPs were assessed with TEM, DLS, and zeta-potential measurements, as well as in imaging phantom studies. The [59Fe]-SPIONs were evaluated …

Reichenbachia, Imperial Edition: Rediscovering Frederick Sander’S Late-Victorian Masterpiece Of Botanical Art, Erica Borey

Theses and Dissertations

This thesis project examines the history, provenance, and contemporary treatment of a rare Imperial Edition of Frederick Sander’s print collection Reichenbachia, Orchids Illustrated and Described, a high-quality orchid compendium dating to the late-nineteenth century. A local philanthropist loaned the Imperial Edition Reichenbachia, number 86 of 100 to Lewis Ginter Botanical Garden in 2011 on a long-term basis as a promised donation. Research into the origins of this collection involves several disparate historical topics, including the Victorian period of “orchid mania,” imperialist business practices, and chromolithographic printmaking. Discussion of the transition of this collection into a museum art collection covers its …

Formation And Analysis Of Zinc Oxide Nanoparticles And Zinc Oxide Hexagonal Prisms And Optical Analysis Of Cadmium Selenide Nanoparticles, Jared M. Hancock

Theses and Dissertations

In this dissertation, methods to synthesize ZnO are reported. First, zinc oxide nanoparticles were synthesized with small amounts of transition metal ions to create materials called dilute magnetic semiconductors (DMS). We employed a low temperature sol-gel method that produces ZnO nanoparticles of reproducible size and incorporates cobalt, nickel, and manganese ions into the nanoparticles. Conditions were controlled such that a range of amounts of Co, Ni, and Mn were incorporated. The incorporation was tracked by color changes in the white ZnO powder to blue for Co, green for Ni and yellow for Mn. XRD measurements showed the nanoparticles were on …

Methods And Metrics For Human Interaction With Bio-Inspired Robot Swarms, Sean C. Kerman

Theses and Dissertations

In this thesis we propose methods and metrics for human interaction with bio-inspired robot teams. We refine the concept of a stakeholder and demonstrate how a human can use stakeholders to lead a swarm as well as switch the swarm between different collective behaviors. We extend the human interaction metrics of interaction time and interaction effort presented in [1] to swarm systems and introduce the concept of interaction effort. These metrics allow us to understand how well the system performs under human influence. We employ systems theory to estimate these metrics, which is useful because this can be done without …

Zinc Environment In Proteins: The Flexible And Reactive Core Of Hiv-1 Ncp7 And The Inhibitory Site Of Caspase-3, A. Gerard Daniel

Theses and Dissertations

Zinc is an essential cofactor of several proteins. The roles of zinc in these proteins are classified as catalytic, structural or regulatory. Zinc present in structural sites is considered to be a chemically inert, static structural element. On the contrary, previous studies on a C2H2 type zinc finger model compound and the C3H type HIV-1 NCp7 C-terminal zinc knuckle have shown that zinc at these sites can undergo coordination sphere expansion under the influence of a Pt based electrophile. The pentacoordination observed around zinc in these experiments raises an important question: are the structural zinc motifs found in the proteins …

Maternal Monitoring And Maternal Psychological Well-Being: Important Components In Treating Conduct Disorder, Benjamin Rosen

Theses and Dissertations

Conduct disorder is characterized by behaviors that take a large toll on the individuals, families, and communities afflicted. Thus, improving treatment effectiveness should be a high priority. Currently, common intervention programs do not address parental depression, even though it has been linked to adolescent conduct disorder behaviors in some studies. The current study assessed whether the relation between maternal depression and adolescent conduct disorder behaviors is mediated by another factor which has been linked to conduct disorder behaviors, maternal monitoring. Results did not support the hypothesized mediated association, but did show significant individual associations for both maternal depression and maternal …

Regulation Of Satiety Quiescence: Cyclic Gmp, Tgf Beta, And The Asi Neuron, Thomas Gallagher

Theses and Dissertations

The worm Caenorhabditis elegans is a well-studied model organism in numerous aspects of its biology. This small free living nematode has less than 1,000 cells, but shows clear conservation in both signaling and behavior to mammals in aspects of appetite control. This is of importance to humans, where failure of appetite control is a major factor in the unprecedented obesity epidemic that we see today. In general, worm behavior reflects its internal nutritional state and the availability and quality of food. Specifically, worms show a behavioral state that mimics aspects of the mammalian behavioral satiety sequence, which has been termed …

The Effects Of Isolation On Endemic Cozumel Island Rodents: A Test Of The Island Rule, Brittany Marie Nuttall

Theses and Dissertations

Island isolation can cause changes in body size, cranial characteristics, and genetic variation in mammals. We use geometric morphometrics to test skull and mandible shape and size change across three species of endemic Cozumel Island rodents in order to test the “island rule” of larger size in isolated rodents. We also sequenced the D-Loop and cytochrome b region of the mitochondrial genome and tested for differences in genetic variation between island and mainland groups, as well as population structure and gene flow in order to assess the effect of island isolation on these three rodents. We found that the three …

Application Of Next-Generation Transcriptomic Tools For Non-Model Organisms: Gene Discovery And Marker Developmentwithin Plecoptera (Insecta), Nicholas Gregory Davis

Theses and Dissertations

Phylogenetic research on non-model organisms has been hindered by limited marker availability. Next generation sequencing techniques are eliminating that barrier. Using Illumina sequencing technology, Trinity assembly software, custom Perl reciprocal BLAST scripts, and Primer3 primer prediction software, we produced and analyzed 7 Plecopteran transcriptomes, representing 7 of the 16 total families, in an attempt to identify and develop conserved orthologous genetic markers. The transcriptomes were used to reconstruct a gene content phylogeny using a simple distance matrix generated from reciprocal blastn data. By producing and filtering a reciprocal blast network we identified and aligned over 450 putative orthologs. Out of …

Habitat Selection By Two K-Selected Species: An Application To Bison And Sage Grouse, Joshua Taft Kaze

Theses and Dissertations

Population growth for species with long lifespans and low reproductive rates (i.e., K-selected species) is influenced primarily by both survival of adult females and survival of young. Because survival of adults and young is influenced by habitat quality and resource availability, it is important for managers to understand factors that influence habitat selection during the period of reproduction. My thesis contains two chapters addressing this issue for K-selected species in Utah. Chapter one evaluates habitat selection of greater sage-grouse (Centrocercusurophasianus) on Diamond Mountain during the critical nesting and brood-rearing period. Chapter two address selection of birth sites by bison (Bison …

Exploring Post-Fire Recovery Of Biocrusts And Desert Ecosystem Services, Jason R. Bahr

Theses and Dissertations

Biocrusts and the ecosystem services they provide are becoming more susceptible to fire as exotic annual grass invasions facilitate the spread of desert wildfires. Further, precipitation patterns across the western United States are predicted to change over the next century, and have the potential to dramatically influence fire regimes and the recovery of burned biocrusts. Despite these changes to desert fire and precipitation cycles, our understanding of post-fire biocrust recovery is limited, especially regarding the first two years after fire. To investigate biocrust recovery, we created burn manipulations (i.e., unburned and burned) and tracked crust form and function over two …

Importance Of Placement Depth In Evaluating Soil Nitrogen, Phosphorus, And Sulfur Using Ion Exchange Resin Capsules In Semi-Arid, Low Fertility Soils, Rachel Lynn Buck

Theses and Dissertations

Ion exchange resin capsules provide a possible alternative to conventional soil testing procedures. Previous studies with semi-arid, low fertility soils observed poor relationships with poorly mobile nutrients such as phosphorus (P). We propose that placement depth may improve those relationships. Our objective was to (1) determine if placement depth could improve resin capsule estimation of the bioavailability of nitrogen (N), P, and sulfur (S) and (2) to determine if resin capsules can effectively estimate S availability in semi-arid, low fertility soils. Field sites were established in Rush and Skull Valleys, Utah on loam and sandy loam soils, respectively. Fertilizer was …

Involvement Of Ampk And Ap-1 Biochemical Pathways In Il-6 Regulation Of Steroidogenic Enzymes In The Adrenal Cortex, Matharage Shenali De Silva

Theses and Dissertations

The adrenal cortex is a crucial endocrine gland in the mammalian stress response. In chronic inflammatory stress, cortisol is elevated whereas adrenal androgens are decreased. Furthermore, ACTH levels have poor correlation with the plasma cortisol in these conditions, thus suggesting that other factors are driving the stress response during chronic inflammatory stress. Interleukin-6 (IL-6), a cytokine which is released during chronic inflammatory stress, is assumed to be one such factor. Thus the biochemical pathways by which IL-6 increases cortisol release from the zona fasciculata (ZF), and decreases adrenal androgen release from the zona reticularis (ZR) were investigated. Since IL-6 activates …

Ferritin-Based Photo-Oxidation Of Biomass For Nanoparticle Synthesis, Bioremediation, And Hydrogen Evolution, Oscar Petrucci

Theses and Dissertations

The cell is the basic unit of all living organisms. It is an amazing machine capable of self-replicating, growing, and synthesizing and shuttling thousands of compounds. To perform all of these activities the cell needs energy. The original source of energy for all living beings is the Sun. The energy of the sun is collected by the autotrophs (mostly plants) through photosynthesis and stored in the chemical bonds of carbohydrates and lipids through carboxylic acid intermediates; animals use these compounds to obtain the energy for their cells. Most of the energy extracted by the cell comes from the citric acid …

Fgf4 Induced Wnt5a Gradient In The Limb Bud Mediates Mesenchymal Cell Directed Migration And Division, John C. Allen

Theses and Dissertations

The AER has a vital role in directing embryonic limb development. Several models have been developed that attempt to explain how the AER directs limb development, but none of them are fully supported by existing data. I provide evidence that FGFs secreted from the AER induce a gradient of Wnt5a. I also demonstrate that limb mesenchyme grows toward increasing concentrations of Wnt5a. We hypothesize that the changing shape of the AER is critical for patterning the limb along the proximal to distal axis. To better understand the pathway through which Wnt5a elicits its effects, we have performed various genetic studies. …

Responding To A Community Tuberculosis Outbreak: Public Health Nursing Interventions, Deborah J. Swanson

Theses and Dissertations

The role of the public health nurse in responding to a tuberculosis (TB) outbreak is instrumental in controlling the disease and preventing further spread in a community. This paper focuses on the interventions carried out by public health nurses in Grand Forks, ND where a TB outbreak occurred from 20 IO until 2013 with a peak in cases identified in late 2012. The population affected had unstable housing, low socioeconomic status, and other risk factors for TB. A total of 26 adult and pediatric cases of TB were identified among a closely connected social network with multiple and prolonged exposures …

Anesthetic Considerations For Phantom Limb Pain, Robert B. Kari

Theses and Dissertations

Title: Anesthetic Considerations for Phantom Limb Pain

Background: Phantom limb pain (PLP) is a phenomenon where pain develops or is perceived in a body part that has been removed. With the prevalence of peripheral vascular disease secondary to diabetes, amputation rates are expected to increase in the next decade. Chronic pain syndromes such as phantom limb pain are difficult to manage and treat

Purpose: The purpose of this project is to detennine if preemptive analgesia for limb amputation is effective in the prevention of PLP

Process: A comprehensive review of the literature was perfonned using multiple …

Parental Role In Educating Adolescents About Alcohol And Chemical Substances: What Is The Best Evidence?, Patricia L. Dumonceaux

Theses and Dissertations

No abstract provided.

Obstacles And Solutions To Studying Functional Adhesives Using Vibrational Sum-Frequency Generation Spectroscopy, Angela Renee Andersen

Theses and Dissertations

Important aspects of adhesion occur at interfaces, including structures that may be different from those in the bulk materials. However, probing the orientation of molecules in functional adhesives poses a significant challenge because adhesive molecules are always located at a buried interface. The limited penetration depth of surface-specific analysis prohibits the study of buried interfaces using those techniques. The large quantity of bulk molecules relative to the adhesive molecules interacting at the interface results in the bulk signal swamping out adhesive signal in bulk analysis techniques. An interface-specific technique is required to study functional adhesives. One such technique that has …

Endocannabinoid-Dependent Long-Term Depression Of Ventral Tegmental Area Gaba Neurons, Jared Mark Weed

Theses and Dissertations

GABA neurons in the ventral tegmental area of the midbrain are important components in the brain's reward circuit. Long term changes in this circuit occur through the process of synaptic plasticity. It has been shown that high frequency stimulation, as well as treatment with endocannabinoids, can cause GABA neurons in the ventral tegmental area to undergo long term depression, a form of synaptic plasticity that decreases excitability of cells. The present study elaborates on the mechanism whereby high frequency stimulation can result in long term depression of ventral tegmental area GABA neurons. Using the whole cell patch clamp technique in …

Water Fat Separation With Multiple-Acquisition Balanced Steady-State Free Precession Mri, Michael A. Mendoza

Theses and Dissertations

Magnetic resonance imaging (MRI) is an important medical imaging technique for visualizing soft tissue structures in the body. It has the advantages of being noninvasive and, unlike x-ray, does not rely on ionizing radiation for imaging. In traditional hydrogen-based MRI, the strongest measured signals are generated from the hydrogen nuclei contained in water and fat molecules.Reliable and uniform water fat separation can be used to improve medical diagnosis. In many applications the water component is the primary signal of interest, while the fat component represents a signal which can obscure the underlying pathology or other features of interest. In other …

Characterization And Processing Evaluation Of Starch/High-Density Polyethylene Materials In Extrusion Blow Molding, Bradley Dale Bacigalupi

Theses and Dissertations

The growing negative impacts of non-biodegradable plastics derived from non-renewable materials have created increasing interest throughout the world for new materials that are both biodegradable and renewable, that can be combined with or replace traditional plastics. Plant-based thermoplastic starch (TPS), a promising alternative material to traditional petroleum based resin, is both biodegradable and renewable and has great potential for use in plastic manufacturing processes. Two major obstacles that prevent more widespread use of TPS include; TPS base material, which is typically manufactured in a flake or powder, is incompatible with standard plastics production equipment that require pelletized resin, the second …

Children's Recognition Of Pride: An Experimental Approach, Darren Jason Garcia

Theses and Dissertations

Pride is elicited when a child takes credit for an achievement or exceeds a socially valued standard or expectation. Evidence suggests that pride has a distinct nonverbal expression that is recognized by adults across cultures (Tracy & Robins, 2004). Research examining when children recognize pride has yielded age discrepancies between studies that use forced-choice response formats and those that use spontaneous-response formats. Differences in children's ability to use and comprehend language may account for some of these differences. The purpose of this thesis was to examine the age at which children reliably recognize pride, while minimizing the need for children …

Adolescent Self-Disclosure And Father Involvement Transactions Across Early To Midadolescence, Stephanie Blickfeldt

Theses and Dissertations

This study investigates the transactional relationship between father involvement and adolescent self-disclosure from early to midadolescence. Four hundred and ninety-six adolescents reported on their general self-disclosure to fathers, and mothers reported on father involvement behaviors and maternal involvement behaviors at ages 11, 13, and 15. Results from a longitudinal cross-lagged model indicated a unidirectional relationship from father involvement to child self-disclosure in both early and midadolescence, and a transactional relationship from adolescent self-disclosure to father involvement in early adolescence. A multiple group analysis by gender revealed that both unidirectional and transactional relationships were significant for boys only. Future research efforts …

Investigation Into The Effects Of Pegylation On The Thermodynamic Stability Of The Ww Domain, Sam S. Matthews

Theses and Dissertations

The covalent attachment of poly(ethylene glycol) (PEG) to a protein surface (known as PEGylation), has been demonstrated to increase the serum half-life of therapeutic proteins by reducing kidney clearance and immunogenicity and by protecting against proteolysis. Theses beneficial effects could be further enhanced if PEGylation consistently increased protein conformational stability (i.e. the difference in free energy between the folded and unfolded states). However, the effects of PEGylation on protein conformational stability are unpredictable; PEGylation has been reported to increase, decrease, or have no effect on the conformational stability of medicinal proteins.This thesis details the results of two studies aimed at …

Regulation Of The Myostatin Protein In Overload-Induced Hypertrophied Rat Skeletal Muscle, Paige Abriel Affleck

Theses and Dissertations

Myostatin (GDF-8) is the chief chalone in skeletal muscle and negatively controls adult skeletal muscle growth. The role of myostatin during overload-induced hypertrophy of adult muscle is unclear. We tested the hypothesis that overloaded adult rodent skeletal muscle would result in reduced myostatin protein levels. Overload-induced hypertrophy was accomplished by unilateral tenotomy of the gastrocnemius tendon in male adult Sprague-Dawley rats followed by a two-week period of compensatory overload of the plantaris and soleus muscles. Western blot analysis was performed to evaluate changes in active, latent and precursor myostatin protein levels. Significant hypertrophy was noted in the plantaris (494 ± …