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Bio-Inspired Design Of Mechanical Band Pass Sensor With The Ability To Scavenge Energy, Md Riaz Uddin Ahmed Dec 2015

Bio-Inspired Design Of Mechanical Band Pass Sensor With The Ability To Scavenge Energy, Md Riaz Uddin Ahmed

Theses and Dissertations

Primary objective of this work is to introduce the multi-scale computational model for the bio-inspired acousto-ultrasonic band pass sensor that are capable of mechanically sense and/or filter wide range user defined frequencies. Selecting a particular and/or a distinct band of frequencies is essential for many applications in science engineering and technologies. For example design of sensors in chemical, biomedical and biological applications; device application for acoustic modulation by breaking the acoustic reciprocity and the sensors used in precision manufacturing applications requires sensing and/or filtering of wide range of acousto-ultrasonic frequencies. Presently, electronic devices are widely employed in commercial applications for …

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Community-Level Factors Associated With Health-Related Quality Of Life Among Older Adults, Yu-Hsiu Lin Dec 2015

Community-Level Factors Associated With Health-Related Quality Of Life Among Older Adults, Yu-Hsiu Lin

Theses and Dissertations

Objective: Health-related quality of life (HRQOL) and well-being is a new topic area for Healthy People 2020 in the U.S. In a broad-based literature review, more research explored individual level factors of HRQOL, and few focused on older adults. Multilevel analysis was seldom adopted to investigate the relationship between area-level socioeconomic or social environment factors and HRQOL. In lieu of the traditional Chapter 4 (Results) and Chapter 5 (Conclusion), two manuscripts representing the two specific research aims are included. The main aim of first manuscript was to explore the association between area deprivation, area health resources and older adults’ HRQOL. …

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A Survey Of The Kinetic Monte Carlo Algorithm As Applied To A Multicellular System, Michael Richard Laughlin Dec 2015

A Survey Of The Kinetic Monte Carlo Algorithm As Applied To A Multicellular System, Michael Richard Laughlin

Theses and Dissertations

We explore the origins and implementation of the Kinetic Monte Carlo method on a system of cells suspended in a liquid media. The situation presented herein has applications in the emerging field of biofabrication, which may have lasting impacts in medical science. The theory behind the method is explained in detail, starting with its emergence in the 1960s, and two major improvements to the scaling of the method are presented, along with a restriction to a special case. Finally, we give the results of several simulations.

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Global Methylation Of Dna Among Spartina Alterniflora Clones Differing In Age At North Inlet, Sc, Trenton Agrelius Dec 2015

Global Methylation Of Dna Among Spartina Alterniflora Clones Differing In Age At North Inlet, Sc, Trenton Agrelius

Theses and Dissertations

“Brown marsh” or “sudden marsh dieback” refers to the rapid death of patches varying in size of Spartina alterniflora. In 2000 following a 100-year record drought, approximately 8,000 hectares of S. alterniflora died along the Louisiana coast, which prompted Louisiana Governor Foster to declare a state of emergency. Other cases have been documented at North Inlet, SC as well as various other marshes along the southeastern United States. Currently, there is much dispute regarding the cause of “sudden marsh dieback” but environmental stress is one of the acknowledged constants across sites. We hypothesized an alternative mechanism in which methylation of …

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Solving Influence Diagrams Using Branch And Bound Search, Arindam Khaled Dec 2015

Solving Influence Diagrams Using Branch And Bound Search, Arindam Khaled

Theses and Dissertations

Influence diagrams (ID) are graphical frameworks for decision making in stochastic situations with mathematical models embedded in them. The goal of an optimal algorithm for an ID is to find a strategy that would maximize the expected utility. We will explain a few algorithms for influence diagrams in this thesis. There exists an obvious temporal ordering among decisions in an ID; and any information used in the past will always be available in the future: these two properties are respectively called the “regularity” and “noforgetting” assumptions. A limited memory influence diagram (LIMID) does not follow these two properties. The existing …

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Secure Cloud Computing For Solving Large-Scale Linear Systems Of Equations, Xuhui Chen Dec 2015

Secure Cloud Computing For Solving Large-Scale Linear Systems Of Equations, Xuhui Chen

Theses and Dissertations

Solving large-scale linear systems of equations (LSEs) is one of the most common and fundamental problems in big data. But such problems are often too expensive to solve for resource-limited users. Cloud computing has been proposed as an efficient and costeffective way of solving such tasks. Nevertheless, one critical concern in cloud computing is data privacy. Many previous works on secure outsourcing of LSEs have high computational complexity and share a common serious problem, i.e., a huge number of external memory I/O operations, which may render those outsourcing schemes impractical. We develop a practical secure outsourcing algorithm for solving large-scale …

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Development Of White Crappie Pomoxis Annularis Reproduction Methods In Closed Aquaculture Systems, Charlie Marcus Culpepper Dec 2015

Development Of White Crappie Pomoxis Annularis Reproduction Methods In Closed Aquaculture Systems, Charlie Marcus Culpepper

Theses and Dissertations

Aquaculture methods are limited for white crappie (Pomoxis annularis), reducing production potential. Therefore, reproduction methods, including induced spawning, sperm cryopreservation and out-of-season spawning, were developed in tank systems. A two week acclimation period (15°C; 3-5 ppt salinity) was necessary to reduce disease-related mortality. Afterwards, four spawning induction hormones and a control were examined to induce spawning. Luteinizing hormone releasing hormone analogue and salmonid gonadotropin releasing hormone analogue performed the best in terms of spawning success and %ertilization. Sperm cryopreservation was effective using Hanks or Ca2+ree Hanks balanced salt solutions with 10%-methanol or 5%-dimethyl-sulfoxide as a cryoprotectant, frozen at 40°C/min. Out-of-season …

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The Development Of A Model For Vascular Calcification And The Effects Of Magnesium Supplementation On In Vitro Calcification, Joshua Nathaniel Grant Dec 2015

The Development Of A Model For Vascular Calcification And The Effects Of Magnesium Supplementation On In Vitro Calcification, Joshua Nathaniel Grant

Theses and Dissertations

Cardiovascular disease is most deadly medical condition in the United States. Medial vascular calcification is a disease that often precedes other more serious cardiovascular diseases that have high mortality. In order to research new therapies for the treatment of medial vascular calcification, an in vitro cell culture model must be developed that mimics the process in vivo. This disease is shown to be an active, cell-mediated process where the vascular smooth muscle cells (VSMCs) in the arteries are differentiating into osteoblast-like cells and depositing hydroxyapatite mineral in the artery walls. By administering inorganic phosphate to cell culture medium, an osteogenic …

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A Mixed-Integer Programming Approach For Jammer Placement Problems For Flow-Jamming Attacks On Wireless Communication Networks, Satish Vadlamani Dec 2015

A Mixed-Integer Programming Approach For Jammer Placement Problems For Flow-Jamming Attacks On Wireless Communication Networks, Satish Vadlamani

Theses and Dissertations

In this dissertation, we study an important problem of security in wireless networks. We study different attacks and defense strategies in general and more specifically jamming attacks. We begin the dissertation by providing a tutorial introducing the operations research community to the various types of attacks and defense strategies in wireless networks. In this tutorial, we give examples of mathematical programming models to model jamming attacks and defense against jamming attacks in wireless networks. Later we provide a comprehensive taxonomic classification of the various types of jamming attacks and defense against jamming attacks. The classification scheme will provide a one …

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A Role Of Dispersed Phase Carbon-Length And Small Amphipathic Coemulsifier In Bovine Serum Albumin Stabilized Nanoemulsions Designed To Deliver Bleaching Agent To Decolorized Fresh Whey, Jingyi Yan Dec 2015

A Role Of Dispersed Phase Carbon-Length And Small Amphipathic Coemulsifier In Bovine Serum Albumin Stabilized Nanoemulsions Designed To Deliver Bleaching Agent To Decolorized Fresh Whey, Jingyi Yan

Theses and Dissertations

Benzoyl peroxide (BP), used to bleach annatto in cheese whey, was encapsulated within the hydrophobic dispersed phase (phi) of nanoemulsions (NEs) to minimize its degradation and extend its efficacy to minimized usage levels. Three purified, saturated short-chain fatty acids of varying chain lengths: butyric (C4), hexanoic (C6) and octanoic (C8) acid, were chosen as the phi to completely dissolve various concentrations of BP. Stabilization was achieved with different concentrations and combinations of primary emulsifier (E), bovine serum albumin, and coemulsifier (CE), Tween 20. Different ultra-high pressures (UHP) were used to generate a stable NE. The best result was made by …

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Verticillium Dahliae Causes The Fungal Wilting Disease Of Cotton Plants Grown On The Mississippi State North Farm, Fahad Mohammedsaleh Albukhari Dec 2015

Verticillium Dahliae Causes The Fungal Wilting Disease Of Cotton Plants Grown On The Mississippi State North Farm, Fahad Mohammedsaleh Albukhari

Theses and Dissertations

The emergence and spread of Verticillium wilt were observed in cotton plants at the R.R. Foil Plant Science Research Center at Mississippi State during the late summer in 2013 and 2014. Several fungi with different morphology and growth characteristics were isolated from diseased cotton plants. Genomic DNA was extracted from the isolated fungal species and used in molecular typing via PCR amplification and DNA sequencing analysis of the ribosomal internal transcribed spacer (ITS) region. A total of five fungal genera were identified, and Verticillium sp. was the most frequently isolated genus. The isolated Verticillium strains could be Verticillium dahliae, Verticillium …

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Characterization Of The Ability Of Yeast Probiotics And Paraprobiotics To Directly Interact With Gram-Positive And Gram-Negative Bacteria, Gabriel Alviola Posadas Dec 2015

Characterization Of The Ability Of Yeast Probiotics And Paraprobiotics To Directly Interact With Gram-Positive And Gram-Negative Bacteria, Gabriel Alviola Posadas

Theses and Dissertations

Yeast probiotics and paraprobiotics, live and inactivated yeast cells, respectively, improve health and performance of livestock by stabilizing the intestinal microbial community. They have also been used for infection prevention and treatment. Despite much research already conducted, the mechanism of direct antagonism, or adhesion of bacteria to the probiotic/paraprobiotic, is under characterized. Additionally, it is unknown which probiotic/paraprobiotic is optimal to use for specific infections. The interactions between the yeast and certain pathogens were analyzed qualitatively with scanning electron microscopy (SEM) and quantitatively with membrane filtration assays. Gram-positive bacteria were found to exhibit specificity under SEM. Through membrane filtration, Listeria …

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Detection Of Flavobacterium Columnare In Tissues And Pond Water Using Real-Time Polymerase Chain Reaction, Gordon Derek Gibbs Dec 2015

Detection Of Flavobacterium Columnare In Tissues And Pond Water Using Real-Time Polymerase Chain Reaction, Gordon Derek Gibbs

Theses and Dissertations

Flavobacterium columnare, a Gram-negative rod-shaped bacterium, is the causative agent of columnaris disease in a variety of fish hosts but is of particular significance to the catfish industry located in the southeastern United States. Columnaris infections are a leading cause of mortalities in catfish ponds, occurring alone or in conjunction with other diseases. Typical diagnostic methods for columnaris infections involve the use of selective media following the observation of gross signs of disease. A real-time quantitative PCR (qPCR) assay to estimate the quantity of bacteria present in environmental and tissue samples was developed and validated. The genetic variability seen in …

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Design, Synthesis, And Testing Of Bis-Corannulene Receptors For Fullerenes Based On Klärner’S Tethers, Peumie Luckshika Abeyratne Kuragama Dec 2015

Design, Synthesis, And Testing Of Bis-Corannulene Receptors For Fullerenes Based On Klärner’S Tethers, Peumie Luckshika Abeyratne Kuragama

Theses and Dissertations

The discovery of the new allotropic forms of elemental carbon (e.g. fullerenes and carbon nanotubes) introduced a novel motif in supramolecular chemistry based on dispersion interactions of curved networks of sp2 hybridized carbon atoms. Buckybowls, the curve-shaped polycyclic aromatic hydrocarbons, appear to be ideal candidates for molecular receptors to recognize fullerenes. Corannulene, the smallest and the best studied buckybowl, has been recognized as one of the important pincers in developing molecular receptors for fullerenes. The main goal of our research is to synthesize corannulene-based molecular receptors for fullerenes with high binding affinities with the use of the pincers preorganized on …

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Subspecies Composition, Genetic Variation And Structure Of Largemouth Bass In Puerto Rico Reservoirs, Nick Ryan Peterson Dec 2015

Subspecies Composition, Genetic Variation And Structure Of Largemouth Bass In Puerto Rico Reservoirs, Nick Ryan Peterson

Theses and Dissertations

Subspecies composition and genetic variation are important to the growth and survival of Largemouth Bass and information on these genetic characteristics is required for proper management of the species. In Puerto Rico, a genetic evaluation is needed to develop management plans for Puerto Rico reservoirs and maintain Florida Largemouth Bass at the Maricao Hatchery. Subspecies composition was characterized among reservoirs and the contemporary Maricao Hatchery broodstock in Puerto Rico, and metrics were compared between subspecies and hybrids among reservoir populations. Genetic variation and structure was determined and compared to Largemouth Bass populations in their native range. Florida subspecies were prevalent …

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Conserving The Mississippi Gopher Frog (Lithobates Sevosa) Through The Use Of Assisted Reproductive Technologies, Katherine Michelle Graham Dec 2015

Conserving The Mississippi Gopher Frog (Lithobates Sevosa) Through The Use Of Assisted Reproductive Technologies, Katherine Michelle Graham

Theses and Dissertations

The Mississippi gopher frog (MGF, Lithobates sevosa) is a critically endangered amphibian which requires the use of assisted reproductive technologies (ART) to successfully reproduce in captivity. Several challenges to successfully breeding MGFs were investigated to improve reproduction in this species. Accurate identification of biological sex is a necessary first step to reproducing species, thus several non-invasive techniques for sex identification were compared in the weakly dimorphic MGF. Reliably obtaining eggs from female MGFs has also proved difficult, therefore, four exogenous hormone therapies were compared for their success to induce ovulation. Ultrasound imaging was used alongside hormone treatments to improve understanding …

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The Effects Of Stress On Physiology And Meat Quality In Cultured Channel Catfish, Mike Ciaramella Dec 2015

The Effects Of Stress On Physiology And Meat Quality In Cultured Channel Catfish, Mike Ciaramella

Theses and Dissertations

Stress during fish culture can impact growth, physiology and fillet quality. Maintenance of high quality seafood is important to ensure the production of a highly marketable product. The present study assessed how sequential stressors affect the physical, physiological, sensory and quality characteristics of channel catfish (Ictalurus punctatus) fillets. Temperature (25°C or 33°C) and dissolved oxygen (DO, ~2 mg/L or >5 mg/L) were maintained for four weeks, followed by socking and transport stress for a total of 12 treatments. After each stage of stress (environment, socking and transport), physical (length, weight and feed consumption), physiological (hematocrit, plasma cortisol, pH, glucose, lactate, …

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The Evaluation Of Adsorbents For The Removal Of Aflatoxin M1 From Contaminated Milk, Erika D. Womack Dec 2015

The Evaluation Of Adsorbents For The Removal Of Aflatoxin M1 From Contaminated Milk, Erika D. Womack

Theses and Dissertations

Taking precautions to restrain aflatoxin M1 (AFM1) from milk is critical, particularly due to the health and economic impact AFM1 imposes. The predominant post-harvest means of reducing AFM1 in milk includes the addition of sequestering agents to feed to diminish the bioavailability of aflatoxin B1 (AFB1), the parent compound of AFM1 found in contaminated feed. Still, residual AFM1 has been found in the milk. Using sequestering agents added to raw milk, we found that activated carbon was the most effective binder to reduce AFM1 contamination. The combination of 0.75% granular activated carbon (GAC) and a flow rate of 0.4 mL/min …

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The Role Of Endocannabinoids In Atherosclerosis, Anberitha Tyiona Matthews Dec 2015

The Role Of Endocannabinoids In Atherosclerosis, Anberitha Tyiona Matthews

Theses and Dissertations

Cardiovascular disease leads in morbidity and mortality in Western societies with no known cure. NADPH oxidase (Nox) contributes to atherosclerosis through the indirect activation of macrophages leading to the internalization of oxidized low density lipoproteins (oxLDL). Chronic inflammation in activated macrophages contributes to atherosclerosis. Because macrophages are positioned at the cross-roads of lipid metabolism in vessel walls, they are important in the cellular pathology of atherosclerosis. Components of the endocannabinoid (eCB) system are vital to atherosclerotic development, since the eCB system has been found to play an important role in the amelioration of atherosclerosis. The eCB system has several components, …

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Promoting The Interest Of African American Teenage Girls In Science: What Can We Learn From An Exemplary African American Science Teacher?, Cynthia Stewart Mcmath Dec 2015

Promoting The Interest Of African American Teenage Girls In Science: What Can We Learn From An Exemplary African American Science Teacher?, Cynthia Stewart Mcmath

Theses and Dissertations

This study focused on science teaching that promoted the interest of African American teenage girls in the science classroom of an exemplary African American science teacher. It focused on, observed and examined the planning, teaching and learning strategies used by the science teacher. It also described what the science teacher experienced during her high school years, during college, during her teaching career. The case study approach/method was used for this research to capture the description and examination of the practices of the science teacher. This research described how an African American female science teacher serves as a role model and …

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Recovery Of Phytophthora Ramorum And Other Phytophthora Spp. In A Forest Adjacent To A Mississippi Ornamental Plant Nursery, Devin Sterling Bily Dec 2015

Recovery Of Phytophthora Ramorum And Other Phytophthora Spp. In A Forest Adjacent To A Mississippi Ornamental Plant Nursery, Devin Sterling Bily

Theses and Dissertations

The movement of the exotic and destructive plant pathogen Phytophthora ramorum into unquarantined areas via the plant nursery trade provides a potential outlet for transmission into eastern United States forests. A two-year survey of Phytophthora species in a forest adjacent to an ornamental plant nursery in Mississippi isolated P. ramorum 20 times from water and once from vegetation, with an additional detection of 14 Phytophthora species and one provisional species. Isolates were recovered from soil, water, and vegetation using baiting and filtering techniques, and verified by their DNA through Polymerase Chain Reaction (PCR) followed by genomic sequencing. This study confirms …

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Reproductive Biology Of The Female Bottlenose Dolphin (Tursiops Truncatus), Holley Stone Muraco Dec 2015

Reproductive Biology Of The Female Bottlenose Dolphin (Tursiops Truncatus), Holley Stone Muraco

Theses and Dissertations

The goal of this long-term study was to better understand the reproductive biology of the female bottlenose dolphin (Tursiops truncatus) and provide a hypothesis for how dolphins may communicate reproductive readiness to one another. Utilizing conditioned dolphins in aquaria, this dissertation examined several previously unknown aspects of dolphin reproduction, including ovarian follicular dynamics during the luteinizing hormone surge, urinary prolactin levels, estrus behavior, vaginal fluid arboriform arrangement, in-situ vaginal and cervical anatomy during estrus, reversed-phase high-performance liquid chromatography (RP-HPLC) of urine samples to identify proteins and peptides that may be used in chemical communication, and a review and anatomical analysis …

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Selection Dynamics In Heliconius Hybrid Zones And The Origin Of Adaptive Variation, Steven Grant Shaak Dec 2015

Selection Dynamics In Heliconius Hybrid Zones And The Origin Of Adaptive Variation, Steven Grant Shaak

Theses and Dissertations

There is repeated evidence that hybridization is a major contributor to the production of adaptive diversity; however, the evolutionary fate of hybrids in natural populations remains poorly understood. In Heliconius butterflies, hybridization is common and responsible for generating a variety of warning color patterns across the genus. Predator avoidance of warning colorations appears to largely be learned, which drives strong positive frequency-dependent selection. This creates a paradox for hybrid lineages: how do novel hybrid forms manage to establish and persist under such strong selection? In this dissertation, I present a series of studies centered on the selection dynamics of Heliconius …

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N-Heterocyclic Carbene Complexes Of Ag(I) And Zr(Iv): Ag(I)-Halide Cubane Type Clusters And The Mechanism Of Hydroamination With -Nhc Pincer Complexes, Wesley D. Clark Dec 2015

N-Heterocyclic Carbene Complexes Of Ag(I) And Zr(Iv): Ag(I)-Halide Cubane Type Clusters And The Mechanism Of Hydroamination With -Nhc Pincer Complexes, Wesley D. Clark

Theses and Dissertations

The synthesis and characterization of the first series of tetra-NHC-Ag(I)-X cubane clusters are reported. The clusters were characterized with 1H and 13C NMR spectroscopy, ESI-TOF MS, and single crystal X-ray diffraction. Crossover experimental data were consistent with intramolecular exchange, which can be visualized by a molecular rotating type mechanism. Additionally, -NHC Zr(IV) pincer type complexes were synthesized and characterized with 1H and 13C NMR spectroscopy, CHN combustion analysis, and X-ray crystallography. A large rate effect was observed based on the halogen and metal center for the hydroamination/cyclization of unactivated aminoalkenes. Zirconium based pincers provided faster reaction rates than their hafnium …

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Nutrient Management In Reblooming Iris 'Immortality', Xiaojie Zhao Dec 2015

Nutrient Management In Reblooming Iris 'Immortality', Xiaojie Zhao

Theses and Dissertations

For its fragrance, showy display and multi-colors, tall bearded (TB) iris (Iris germanica L.) has great potential as a specialty cut flower. This study was conducted to investigate the optimum nutrient management, especially nitrogen (N), of reblooming TB iris 'Immortality'. The objectives were to investigate the effects of N rate and form and phosphorus (P) rate on growth, flowering, and nutrient uptake, and to assess seasonal changes in the composition of nitrogenous compounds and carbohydrates. In general, greater N rates increased plant height, leaf SPAD reading, the number of inflorescence stems, plant dry weight, plant N content, and uptake of …

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Willingness Of Mississippi's Nonindustrial Private Forest Landowners To Manage Forests For Ecosystem Services, Edward Mutandwa Dec 2015

Willingness Of Mississippi's Nonindustrial Private Forest Landowners To Manage Forests For Ecosystem Services, Edward Mutandwa

Theses and Dissertations

Private forest land in Mississippi provides a wide range of ecosystem services. This study examined nonindustrial private forest (NIPF) landowner familiarity with ecosystem services and conservation programs and quantified the proportion of forest land they were interested in managing for ecosystem services as well as determined compensation levels required for implementing forest management strategies featuring management restriction to facilitate production of multiple ecosystem services. The data collection process was based on a structured questionnaire administered to 2,025 NIPF landowners in Mississippi. Findings indicated that landowner familiarity with conservation programs was low. However, they were familiar with ecosystem services found in …

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Preventing Teacher Attrition: Expert Teachers' Sources Of Stress And Coping Strategies, Katherine Damaris Garth Dec 2015

Preventing Teacher Attrition: Expert Teachers' Sources Of Stress And Coping Strategies, Katherine Damaris Garth

Theses and Dissertations

Teaching is considered to be one of the most stressful occupations (Johnson et al., 2005). Not only is the profession highly stressful, it has the highest degree of career turnover of any profession (Ingersoll, 2001). Consistent stress can lead to burnout of the profession. McCarthy et al. (2009) noted that teacher burnout can be a result of inappropriate coping resources. The purpose of this study was to investigate the sources of stress, coping strategies, and learned coping strategies among expert elementary teachers. The sources of stress and coping strategies were examined using the Social Ecological Model which allowed for sources …

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Evaluation Of A Cultural Practice And 2,4-D-Based Herbicide Programs For Glyphosate-Resistant Palmer Amaranth Management, Benjamin Haynes Lawrence Dec 2015

Evaluation Of A Cultural Practice And 2,4-D-Based Herbicide Programs For Glyphosate-Resistant Palmer Amaranth Management, Benjamin Haynes Lawrence

Theses and Dissertations

Glyphosate-resistant Palmer amaranth (Amaranthus palmeri [S.] Wats) is an economically troublesome weed to southeastern United States soybean (Glycine max [L.] Merr.) growers. Palmer amaranth is troublesome due to its evolution of resistance to multiple herbicide modes of action, competiveness, and prolific seed production. Greenhouse studies were conducted at the Delta Research and Extension Center in Stoneville, MS to evaluate different rates of 2,4- dichlorophenoxyacetic acid (2,4-D) for control of Palmer amaranth. Field experiments were conducted at the Delta Research and Extension Center in Stoneville, MS in 2013 and 2014 to evaluate Palmer amaranth emergence using a cultural practice and a …

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Antioxidative Efficacy And Relative Accessible Hydrophobicity Of Aromatic Residue Rich Peptides In Alfa-Chymotryptic Digests Of Acid Casein, Wenjie Shao Dec 2015

Antioxidative Efficacy And Relative Accessible Hydrophobicity Of Aromatic Residue Rich Peptides In Alfa-Chymotryptic Digests Of Acid Casein, Wenjie Shao

Theses and Dissertations

Four casein-derived peptides fractions of varying hydrophobicity were obtained from á-chymotryptic digest of acid casein using hydrophobic interaction chromatography, termed fractions one through four (abbreviated, F1, F2, F3, and F4). Four standard methods involving alkoxyl, peroxyl, 2, 2-diphenyl-1-picrylhydrazl (DPPH), and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ABTS•+ radicals, were used to measure antioxidative properties. While significantly superior efficacy was exhibited by F2 for all tests except against DPPH, no correlation between antioxidant efficacy and surface hydrophobicity was found. By using capillary electrophoresis and high performance liquid chromatography, the detection of aromatic chromophores by ultraviolet at 280 nm in the fractions revealed that …

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Thermodynamic Studies Of Zn2+ Binding To Glutathione, Madhubhashini Lakdusinghe Dec 2015

Thermodynamic Studies Of Zn2+ Binding To Glutathione, Madhubhashini Lakdusinghe

Theses and Dissertations

Glutathione is one of the most abundant organic compounds found in many biological systems. It is a non-protein tripeptide of Glutamic acid, Cysteine and Glycine. Due to its stability, high cellular concentrations and structural features like gammaglutamyl linkage and sulfhydryl group, glutathione involves with many biological pathways including: cellular defense against xenobiotics and naturally occurring deleterious compounds, like free radicals, metal sequestering, and maintaining cellular sulfhydryl status. Glutathione has been broadly studied however the literature associated with Zn2+ coordination is not clear. This study is focused on collecting thermodynamic data of glutathione binding to Zn2+ metal ions using calorimetric technique. …

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