Digital Commons Network^™

Psychometric Developments Of The Ace-Iq: Understanding The Trauma History Of Latine Immigrants, Jorli K. Swingen Dr.

Theses and Dissertations

Adverse childhood experiences (ACEs) have a profound effect on an individual’s physical and mental health. The World Health Organization has recently updated the ACE questionnaire so it could be used with international populations. The Adverse Childhood Experiences-International Questionnaire (ACE-IQ), has not been translated to Spanish or used with Latine immigrants.

This study translated the ACE-IQ into Spanish, evaluated the psychometric properties of the questionnaire, and collected data on 184 four adult English Language Learners in Chesterfield County, VA.

There is evidence of internal consistency for the ACE-IQ as a whole (α = .908) and within subscales. Three factors were identified …

A Cost-Effectiveness Analysis Of Early Literacy Growth In Public Pre-Kindergarten Programs, Bruce A. Fillman

Theses and Dissertations

Student early literacy readiness is predictive of later academic success and pre-k programs are a proven intervention to develop early literacy; however, the cost of pre-k programs differs greatly. These differences in cost are related to the opportunities and services included within each program. This study examined four pre-k programs offered within a large school system in Virginia to determine if there were differences in students’ early literacy growth by program attended and evaluated the ratio of cost to achievement of each pre-k program to determine their cost-effectiveness. To do this, two one-way analyses of variances (ANOVAs) were conducted to …

Repeat Full Mouth Dental Rehabilitation Under General Anesthesia For Medicaid Eligible Healthy Children Who Are High Caries Risk, Lajoi E. Wiggins

Theses and Dissertations

Purpose: To evaluate the treatment of healthy children who are high caries risk and of low socioeconomic status and who require repeat full mouth dental rehabilitation (FMDR) under general anesthesia (GA).

Methods: A retrospective chart review was performed on healthy Medicaid eligible patients who were initially between 18 months - 6 years of age who underwent FMDR under GA in the OR more than once at the Virginia Commonwealth University (VCU) School of Dentistry, Department of Pediatric Dentistry from January 2008 to December 2018. Biographical information for each patient was gathered into a REDCap™ Survey. Statistical analysis, chi-squared test with …

Strategies And Devices For Improving Respiratory Drug Delivery To Infants And Children With Cystic Fibrosis, Karl Bass

Theses and Dissertations

Cystic Fibrosis (CF) is a degenerative disease, which causes thickening of the airway surface liquid and reduced mucociliary clearance, which provides an ideal habitat for bacterial infections. Early treatment of CF in children can prevent chronic infection, improve quality of life, and increase life expectancy. The most predominant bacteria found in CF-diseased lungs is Pseudomonas aeruginosa (Pa), which can be treated with inhaled tobramycin. Excipient enhanced growth (EEG) powder formulations are well suited for administering tobramycin to children, as the EEG approach provides minimal upper airway loss and targeted drug delivery. This method uses an initially small aerosol for high …

Examining Body Mass Index And Sepsis Mortality At One Year After Sepsis, Jamie D. Robinson

Theses and Dissertations

Introduction: Sepsis is a serious and life-threatening syndrome affecting 1.7 million Americans annually and resulting in approximately 270,000 deaths. An “obesity paradox” where obese individuals have lower sepsis mortality than their non-obese counterparts has been described. The problem is that the longevity of the effect is unknown, and few studies have examined the obesity paradox after 1-2 months post-diagnosis.

Methods: This retrospective cohort study examined clinical, demographic, and biomarker variables thought to affect sepsis mortality at three-time points: 30 days, 180 days, and at one year post-sepsis diagnosis in order to shed light on specific factors that might define …

Reaction Profiling Of Extracellular Protein Phosphorylation Through A Microfluidic Reactor Coupled With Raman Spectroscopy, Abigail Casey

Theses and Dissertations

Diseases and disorders in the human body are considered abnormalities of proper cellular function. Understanding the signal transduction mechanisms that cause these abnormalities is crucial to developing earlier detection methods, better treatment options and effective cures. While current diagnostic procedures are powerful tools in diagnosing diseases, they are ineffective in informing physicians on the real-time behavior of the signal transduction mechanisms associated with diseases and disorders. Currently, disease progression is monitored over time through routine patient visits and testing by one or more of the above techniques. By developing an approach that can monitor structural and conformational changes of proteins …

Education Support For Foster Care Youth: The Impact Of Federal Spending On Employment Outcomes, Carlisle C. Bates

Theses and Dissertations

This study examined the extent to which supportive services funded through the federal John H. Chafee Foster Care Independence Program (CFCP) are associated with academic success and employment in foster care youth. Studies suggest that this group experiences poorer employment outcomes (Gypen, Vanderfaeillie, De Maeyer, Belenger, & Van Holen, 2017; Okpych & Courtney, 2014) and earn less money annually (Gypen et al., 2017; Okpych & Courtney, 2014; Pecora, 2012). Despite a 29.4-billion-dollar annual budget for foster care services, individuals with a foster care history struggle after they transition out of care.

The CFCP is intended to help states improve education …

Characterization Of The Gonococcal Calprotectin Zn-Acquisition System, Tdfh, And The Efficacy Of Metal Starvation For Treating Gonococcal Disease, Michael T. Kammerman

Theses and Dissertations

Abstract

Characterization of the Gonococcal Calprotectin Zn-Acquisition System, TdfH, and the Efficacy of Metal Starvation for Treating Gonococcal Disease.

By Michael T. Kammerman, B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University

Virginia Commonwealth University, 2020

Major Director: Cynthia Nau Cornelissen, Ph.D.

Professor, Department of Microbiology and Immunology

Neisseria gonorrhoeae, the bacterial agent that is responsible for the human disease gonorrhea, has had a steady increase in the number of infections per year. In 2018 the WHO estimated over 87 million infections occurred world-wide, and the CDC …

Effects Of Gestational Ozone Exposure On Privileged Placental And Brain Barrier Integrity, Alexander I. Hamm

Theses and Dissertations

Ambient outdoor ozone, a common of component of photochemical smog and urban air pollution, is linked to various neurological and vascular pathologies. Its immediate reaction with lung surfactant after inhalation results in complete reactivity of the gas, with no active ozone passing into circulation. This indicates the presence of secondary and tertiary mediators in ozone-related systemic pathologies after pulmonary insult. In vasculature, ozone exposure is associated with an acute hypertensive phenotype apparent at least 24 hours after dose, such as experienced on a hot summer afternoon in a large metropolitan area like Los Angeles or Mexico City. However, the effects …

Modeling Gardnerella Host-Pathogen Interaction: Characterization Of Vaginolysin And The Opacity Phenotype, Erin M. Garcia

Theses and Dissertations

Bacterial vaginosis (BV) describes a dysbiotic state of the vaginal mucosa, during which the dominance by beneficial lactobacilli species is lost, the vaginal pH increases, and the flora comprising the vaginal microbiome becomes more diverse. Bacterial taxa associated with this dysbiotic state interact to form a dense polymicrobial biofilm on the surface of the vaginal epithelium. This shift in the vaginal microflora is clinically important, even when symptoms are not noted, as women with BV are at increased risk for acquisition of other sexually transmitted infections, including HIV, and are more than two times as likely to experience preterm birth. …

Towards A Greener Route To Blockbuster Statin Drugs: Harnessing The Power Of Biocatalysis And Integrating Cross-Coupling Catalysis In The Synthesis Of Rosuvastatin, Chanaka M. Amarasekarage

Theses and Dissertations

As global and political awareness about green and sustainable practices sharpens in the face of dramatic climate change and environmentally detrimental practices, one attractive approach is to harness the catalytic potential of enzymes. Thus influenced, our efforts focused on the development of an innovative chemoenzymatic route to widely prescribed Blockbuster statin drugs, specifically rosuvastatin. In this work, we engineered novel deoxyribose phosphate aldolase (DERA) variants via rationally designed site-directed mutagenesis to accept non-natural, less polar substrate, acrolein, along with acetaldehyde. Several of new mutants displayed elevated tolerance towards aldehydes and showed effective incorporation of acrolein into the unprecedented enzymatically-derived chiral …

Examining The Role Of Racial Discrimination In Obesity Treatment Disparities Among African American Emerging Adult Women, Kristal Brown

Theses and Dissertations

The overarching goal of the proposed work was to examine how racial discrimination contributes to disparities in adiposity and treatment response between non-Hispanic Black and non-Hispanic White emerging adult women, and to identify potential protective and risk factors that might moderate this association—all in service of improving lifestyle interventions for this high-risk population. Project aims were achieved across three studies. In Study 1, we extracted data for emerging adults (N=261, BMI=33.8+4.9 kg/m², age=21.9+2.1 years) who recently completed a BWL program adapted specifically for emerging adults and compared treatment outcomes for non-Hispanic White and non-Hispanic Black women. In Study …

Model-Based In-Vitro Pk/Pd Profiling Of Novel Synthetic Allosteric Effectors Of Hemoglobin (Aeh) As Potential Sickle Cell Disease (Scd) Therapeutics, Xiaomeng Xu

Theses and Dissertations

Introduction: Allosteric effectors of hemoglobin (AEH) represent a class of synthetic aromatic aldehydes that transiently form covalent interactions (Schiff-base) with hemoglobin (Hb) to form Hb-AEH adduct, preventing the HbS polymerization and sickling of red blood cells (RBC). The overall objective of this research was to aid in the optimization of novel AEH by understanding their target-site disposition of AEH in relevant biological matrices, e.g., HbA solution, whole blood (WB) and human liver cytosol (HLC), a surrogate of aldehyde dehydrogenase (ALDH)-mediated oxidative metabolism.

Methods: A “universal” HPLC-UV/Vis assay method was developed for the quantitation of HbA-AEH adduct for chemically …

Integration Of Multi-Omic Platforms To Understand The Regulation Of Genes In Human Disease, Eva Lancaster

Theses and Dissertations

Epigenetic mechanisms have been hypothesized to modify gene expression (GE) in response to both genetic variation and environmental exposures, ultimately contributing to the development of complex traits. Epigenome-wide association studies (EWAS) aim to test the theory that marks of DNA methylation (DNAm) have downstream consequences that result in the development of complex diseases. Although these methods have been successful in identifying DNAm patterns in complex traits, specific molecular mechanisms involved in the etiology of complex diseases remain unidentified. This knowledge gap does not originate from a lack of DNAm-trait associations, but rather stems from study design issues that affect the …

The Impact Of Occupational Therapy Acute Care Services On Readmission Rates For Patients In Medicare’S Hospital Readmission Reduction Program, Jessica M. Edelstein

Theses and Dissertations

The United States (US) health care system is faced with the daunting challenge to make healthcare payments commensurate with quality of care provided. To assess quality, metrics for reimbursement have been established by Medicare. One such quality metric is hospital readmissions (readmissions). Readmissions are associated with poor patient outcomes and costly. Associated poor patient outcomes include higher risk for mortality, deconditioning, nutritional issues and cognitive impairments. As a result, readmissions cost Medicare $26 billion annually. Current strategies for reducing readmissions in the US are fragmented and hospital-specific. While specific strategies may vary, hospitals that have low readmissions rates tend to …

Characterizing Patterns Of Cannabis Use And Related Health Effects Among Young Adults Using Ecological Momentary Assessment, Alyssa Rudy

Theses and Dissertations

Introduction: Rapid changes in cannabis use policy and prevalence, particularly among young adults, and the lack of information regarding cannabis harms warrant investigation regarding the daily use patterns of cannabis users. Little is known regarding how variability in cannabis use frequency and administration method use patterns may be associated with differential acute cannabis-related health effects (i.e., subjective intoxication and respiratory symptoms). The purpose of the study was to characterize daily and weekly cannabis use patterns and associated cannabis-related intoxication and respiratory symptoms and test the interactions of cannabis use frequency and cannabis administration method use on cannabis-related intoxication and respiratory …

The Pseudomonas Aeruginosa Interactome: Insights Into Srna-Mediated Regulatory Networks, Christine M. Van Duyn

Theses and Dissertations

Pseudomonas aeruginosa is a Gram-negative bacterium found in various environmental niches including soil, water, hospital environments, and within a broad range of hosts. It is well known for its metabolic versatility and intrinsic and acquired resistance to a variety of antimicrobial agents. Moreover, this bacterium has a remarkable ability to adapt and survive in suboptimal environments by altering its transcriptional profile in response to nutrient deprivation, changes in temperature and pH, osmotic stress, the presence of reactive oxygen species (ROS), and exposure to antibiotics and host defenses. P. aeruginosa colonizes individuals with compromised immune systems and severe burn injuries and …

The Impact Of Self-Efficacy, Teacher Mentoring And Administrative Support On Teacher Retention In Title 1 Schools, Holly Cwiklinski

Theses and Dissertations

Information Security Policy (ISP) compliance is crucial to the success of healthcare organizations due to security threats and the potential for security breaches. UNIX Administrators (UXAs) in healthcare Information Technology (IT) maintain critical servers that house Protected Health Information (PHI). Their compliance with ISP is crucial to the confidentiality, integrity, and availability of PHI data housed or accessed by their servers. The use of cognitive heuristics and biases may negatively influence threat appraisal, coping appraisal, and ultimately ISP compliance behavior. These failures may result in insufficiently protected servers and put organizations at greater risk of data breaches and financial loss. …

Doctor-Patient Communication: The Experiences Of Black Caribbean Women Patients With Diabetes, Rosanne Paul-Bruno

Theses and Dissertations

This applied dissertation was designed to examine the verbal and non-verbal communication experiences of Black Caribbean diabetic women patients with their doctors, in order to provide a better understanding of the essential aspects of doctor- patient communication and their experiences as they managed their condition. Black Caribbean women have been disproportionately impacted by medical conditions such as diabetes. It has been a documented fact that minorities experience disparities in the health care system at different levels and doctor-patient communication is no exception. Poor doctor-patient communication has been known to hinder patients’ health outcomes, and therefore warrants such studies to increase …

Haitian Centenarians: An Undocumented Phenomenon, Erna Dasilma

Theses and Dissertations

A ceaselessly neglected phenomenon until recent years, especially in developing countries, centenarians bring unique wisdom, perspective, history and science to our society based on their lived experiences. Although there is a strong interest in the experiences of individuals who are over 100 years old, few formal research studies have been conducted concerning centenarians, principally in developing countries. One of the central goals of conducting this study was to bridge the gap in the literature when it comes to centenarians in less developed countries, given the lack of reported statistics, with emphasis given to those living on the battered, but resilient …

Perceived Enablers And Barriers To The Implementation Of Pbis In A Rural Elementary School, Katherine Mechele Woodall

Theses and Dissertations

Embedded within the 1990 amendment of the Individuals with Disabilities Education Act (IDEA) was a mandate stipulating all school systems incorporate some form of PBIS. Elected officials thought it was necessary to implement positive behavior intervention and supports (PBIS) into school systems behavior plans due to the ever-increasing number of students with mild to severe behavioral issues. Behavior issues include truancy, verbal and physical abuse of teachers and peers, and depression, to name a few. These behavior issues negatively impact a student’s ability to make satisfactory academic progress as well as negatively impacting the learning of their peers.

Yet, despite …

A Comparative Study On Parental Involvement, Roldens Paulynice

Theses and Dissertations

Parental involvement has been found by many researchers to be important in student academic success. However, many school leaders are challenged to get many parents actively involved in their child’s education. At the research site, for example, the researcher observed that many parents do not get actively involved in their child’s education, and they miss the school parental meeting or programs. If this problem is solved, school staff members or leaders would care and would benefit from it. The purpose of this study was to explore the perception of educators on the impact of parental involvement on student success and …

Engineering Pathological Microenvironments For Cardiovascular Disease Studies, Ojaswee Adhikari

Theses and Dissertations

Food insecurity is a growing issue in the United States. Iron deficiency is the most common form of nutritional deficiency in patients with endothelial dysfunction and vascular-related diseases. This preliminary study lays the groundwork for the “Nutrient deficiency-on-a-chip” model. Endothelial cells are cultured on mechanically tunable, enzymatically cross-linked gelatin and treated with deferoxamine, an iron chelator, or angiotensin II were used to simulate a nutrient deficient and diseased environment, respectively. As oxidative stress and disturbed barrier function are the most prevailing mechanism of angiotensin II and iron deficiency induced endothelial dysfunction, to test our model we investigated the changes in …

The Effect Of Maternal Nutrition And Genetic Background On Myogenic And Adipogenic Development In Skeletal Muscle Of Angus And Brahman Cattle Offspring, Racheal L. Lemire

Theses and Dissertations

The objective of this study was to determine breed specific effects of nutritional insults during gestation on regulation of muscle and adipose tissue growth in beef cattle offspring during fetal and early postnatal development. Angus and Brahman heifers were randomly assigned to 1 of 2 dietary treatments consisting of 100% or 60% based on net energy requirements for gestating heifers. Nutritional treatments were imposed from day 50 to 180 of gestation. Fetuses harvested at 180 days of gestation had smaller frames and spared critical organs. Gene expression in several fetal tissues indicated potential for compensatory growth. Postnatally, Brahman offspring weighed …

Rice (Oryza Sativa) Response To Sub-Lethal Concentrations Of Crop Desiccants, Justin M. Mccoy

Theses and Dissertations

Research was conducted at the Mississippi State University Delta Research and Extension Center from 2016 to 2018 to determine the effects of sub-lethal concentrations of paraquat, glyphosate, saflufenacil, and sodium chlorate exposure to rice at late-season growth stages, determine the effects of exposure to sub-lethal concentrations of glyphosate or paraquat on multiple rice cultivars, and characterize the effects of paraquat exposure and Oebalus pugnax feeding on rice grain quality. In the current research, rough rice grain yields were reduced by exposure to sub-lethal concentrations of soybean desiccants 0 to 28 DAH. Rice injury was determined to not be an accurate …

Perceptions Of Food, Supportive Resistance Resources, And Nutrition Knowledge Of Bariatric Surgery Patients, Savannah K. Walker

Theses and Dissertations

Adult overweight and obesity is a life-threatening health concern worldwide. Bariatric surgery is a successful treatment for severely obese individuals however, there is a moderate risk for weight regain. This study evaluated perception of food, supportive resistance resources, and nutrition knowledge, which play important roles in successful weight loss post bariatric surgery. Participants completed a three-part questionnaire at three time intervals: prior to surgery, 2 months post-surgery, and 5 months post-surgery. Results show that bariatric patients’ eating behavior significantly changes post bariatric surgery, but a disordered perception of food remains. Bariatric surgery patients believe support from significant others and family …

Characterization Of Fatty Acid Composition Of Bull Sperm With Varied Cryotolerance, Holly Evans

Theses and Dissertations

The objectives of this study were to determine fatty acid composition and acrosome status from bull sperm with different freezabilities (n = 12). We hypothesized that lipid fractions had differentiated fatty acid compositions and such differences influence sperm freezability and the sperm acrosome. Fatty acids were extracted from fresh frozen sperm and fractionated by solid-phase extraction. Thirtyour fatty acids were quantified. Saturated fatty acids were predominant, accounting for 71 to 80% of fatty acids in both fractions. Differences in composition between fractions existed (P < 0.001). Branched chain fatty acid concentrations (15 to 18 µg) were almost twice that of polyunsaturated fatty acid concentrations found in the polar fractions (8 to 9 µg; P < 0.001). Sperm with differentiated freezabilities had few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). Analyses of acrosome status of sperm revealed that acrosomes were affected differently among bulls.

Effect Of Pro- And Prebiotics On The Apparent Digestibility Of Nutrients And Identification Of Fecal Bacterial Isolatew In The Sedentary And Exercising Horse, Courtney Heaton

Theses and Dissertations

The primary objectives of the following experiments were to: 1) determine the impact of a directed microbial (DFM) blend on digestibility and microbial populations on horses fed low (LQ) and high (HQ) quality hay, 2) evaluate the differences in diet digestibility, microbial populations, and blood metabolites due to DFM supplementation to the exercising horse, 3) assess the impact of short-chain fructooligosaccharide (scFOS) supplementation on digestibility and the fecal microbial ecosystem in senior (SR) vs. mature (MA) horses, and 4) determine the relationship between insulin dysregulation (ID) and morphometric neck measurements (MNM) in the non-obese stock-type horse. Results indicated that: 1) …

Effects Of The In Ovo Injection Of Vitamin D3 Sources On The Hatchability, Performance, Breast Meat Yield, Small Intestine Morphology, And Immunity Of Ross 708 Broilers Subjected To Dietary Insufficiency And Pathogenic Agents, Seyed Abolghasem Fatemi

Theses and Dissertations

Effects of varying dosages (0.6 to 4.8 μg/egg) of vitamin D3 (D3) and 25-hydroxycholecalciferol (25OHD3), administrated by in ovo (amniotic) injection, on various embryonic and post-hatch physiological variables in Ross 708 broilers were investigated. The in ovo injection of 1.2 to 4.8 μg of both D3 and 25OHD3 increased serum 25OHD3 concentrations in broiler embryos in comparison to non-injected and diluent-injected control groups. Furthermore, the effects of 2.4 μg of D3 and 25OHD3 alone and in combination on the performance, meat yield and quality, small intestine morphology, and immunity of the broilers fed a diet restricted in calcium and phosphorous …

Understanding The Effects Of Mass Mortality Events On Plant Communities And Consumer Behavior, Carolina Baruzzi

Theses and Dissertations

Mass mortality events (MMEs) are die-offs that result in increased carrion biomass and sometimes the impairment of functional roles. Concurrently, several vulture species are declining. Carrion is a basal resource in ecosystems and its recycling by vultures is considered an ecosystem service. However, the consequences of simultaneously increasing carrion loads and declining vulture populations are unknown. I developed a theoretical framework predicting that, with increasing carrion biomass, carrion food web diversity would increase horizontally and vertically, respectively increasing and decreasing carrion recycling efficiency. Using a manipulative experiment, I investigated the role of bottom-up and top-down forces affecting plant communities during …