Digital Commons Network^™

Purification Of Recombinant His6-Gfp-Preptin Fusion Protiens, Apoorva Kollaram

Theses and Dissertations

Preptin, a 34-amino-acid peptide derived from proinsulin-like growth factor II, has emerged as a promising therapeutic candidate for both Type 2 diabetes mellitus and osteoporosis. Its dual functionality—stimulating insulin secretion without inducing hypoglycemia and promoting osteoblast proliferation—positions it as a unique alternative to traditional sulfonylureas and osteogenic agents. In this study, we developed a purification protocol for green fluorescent-preptin fusion proteins. These fusion proteins will be used to investigate the functional significance of residues Pro17, Val18, Gly19, Trp27, Lys28, and Gln29 in rat preptin, which shares high sequence similarity with the human form. Site-directed mutagenesis was performed using NEB Q5 …

Qlorax: Heuristic-Guided Fine-Tuning Of Llama-2 For Domain Adaptation In Entrepreneurship, Gaurob Saha

Theses and Dissertations

This thesis presents a study on the fine-tuning of large language models (LLMs) for domain-specific applications using limited data. We fine-tuned the LLaMA-2 (7B) model on a curated entrepreneurial dataset containing 3,545 human-written question-answer pairs, of which 3,095 were used for training and 450 were reserved for evaluation. A complete fine-tuning and evaluation pipeline was developed, which included clustering human-written answers, generating centroid-based summaries for each cluster, and evaluating the model's generated responses through cosine similarity.

Training was carried out over five epochs, with model performance evaluated after each epoch. The fine-tuned model demonstrated strong semantic alignment with human-written content, …

Exploratory Data Analysis (Eda) And Predictive Machine Learning (Ml) For Buildings’ Energy Fault Detection, Akshith Nukala

Theses and Dissertations

Building energy load fault detection is a critical challenge in energy usage analysis. It helps uncover energy wastage, machinery/appliance degradation or inefficiency, and failures or faults in buildings’ HVAC (heating, ventilation, and air conditioning) systems. Early identification of machinery failure and energy wastages due to operational maintenance negligence in large sites such as campus buildings is indispensable for achieving energy efficiency. This is crucial for saving patrol and minimizing the response time to restore the building appliances or systems to their optimal state.

Advancements in state-of-the-art AI/ML data-driven algorithms and techniques enabled us to build accurate, efficient and scalable fault …

The Impact Of Pre-Kindergarten Programs On Literacy Achievement In Primary Grades In Rural Southeast Alabama Title I Schools, Courtney Sonntonya Thorton

Theses and Dissertations

The purpose of this study was to examine how pre-kindergarten impacts the literacy achievement of students in Alabama Title I schools. This quantitative study employed a causal-comparative (ex post facto) research design to examine the impact of Alabama’s First-Class Pre-K (FCPK) program on early literacy and school readiness, as measured by the Dynamic Indicators of Basic Early Literacy Skills (DIBELS) and the Alabama Early Learning Inventory (AL ELI). The design was selected due to its appropriateness for studying naturally occurring groups and its capacity to explore potential cause-and-effect relationships without researcher-controlled intervention.

The independent variable was Pre-K attendance (attendee vs. …

Exploring Brent-Kung Adders In Balanced Ternary Cmos Logic, Astrea J.C. Rojas

Theses and Dissertations

The modern computer operates on a 64-bit architecture. These devices can store large numbers and precise decimals, but more advanced devices are needed to support progressing technologies every day. A more efficient system with higher speeds and larger operable numbers would be a key to optimization of computation as we know it. The ternary device, operating in base-3, has the potential to be that optimization. However, binary technology has such precedent and research that it is a difficult gap to span to compare the ternary system to the modern binary system. With a more advanced adder and optimized gates using …

An Analysis Of Coal Ash Pollution In The State Of Georgia, Mick W. Etchison

Theses and Dissertations

This social science paper will explore the factors contributing to the prevalence of coal ash in specific regions of Georgia and its impact on local ecosystems and human health. It provides an analysis of the current environmental and political issues surrounding the containment and spread of coal ash throughout the State. Coal ash is primarily found in the central, southwestern, and northeastern regions where coal-fired power plants and coal ash storage sites are located. The paper will then discuss the political, economic, and legal reasons behind the lack of effective regulation and mitigation of coal ash spread. Coal ash illustrates …

Cultivating Mindsets And Transformative Practices Of Beginning Alternative Certified Teachers: A Teacher Perception Study, Rodney Keith Wilkerson

Theses and Dissertations

The shortage of qualified teachers in the United States has been a persistent issue for decades. The number of qualified candidates to fill teaching vacancies has fallen short as the number of teachers needed to educate today continues to increase (Carothers et al., 2019). Traditionally, teacher preparation programs were the primary source of certified teachers. However, these programs have been decimated by declining student enrollment and by criticisms that they do not meet the needs of prospective teaching candidates today (Darling-Hammond, 2020). In recent years, the shortage has become even more acute, with some regions facing severe teacher shortages (Carothers …

The Relationship Of Preschool Variables To Kindergarten Early Literacy Skills, Michele Davis Thomason

Theses and Dissertations

This dissertation examines the impact of Pre-K experiences on kindergarten early literacy skills in Alabama. A convergent parallel mixed-methods research design was utilized to analyze assessment data from My Teaching Strategies AlaKiDS and Renaissance STAR Early Literacy for kindergarten students in a rural Alabama district. The students were divided into three groups for comparison: students who attended Alabama First Class Pre-K, those in private programs using the Abeka curriculum, and students with no formal Pre-K experience. The dissertation utilized Vygotsky’s constructivist theory to assess the influence of early educational environments on the cognitive development through scaffolding and the Zone of …

Energy Systems Optimization Techniques In The Oil And Gas Industry With Special Attention On Oil Field Reciprocating Compressors, Sean Jainarine

Theses and Dissertations

The oil and natural gas exploration and production industry is undergoing rapid growth, resulting in an increased number of operational wells and heightened potential for air emissions from oil and natural gas sources. Within production sites, compressors play a pivotal role and are often regarded as the "heart of a manufacturing plant" in the oil and gas sector. These crucial devices are designed to compress air or other gases to pressures higher than atmospheric pressure. Gas compression is typically achieved using devices with rotating blades or cylinders featuring reciprocating compressors while are essential for operations demanding high-pressures in small packages. …

Understanding The Mentorship Experiences Of Underrepresented Minority Stem Leaders, Elias Saade

Theses and Dissertations

There is a growing need to diversify the science, technology, engineering, and math (STEM) workforce to better address complex societal challenges and drive innovation. However, persistent disparities limit the retention and advancement of underrepresented minorities (URMs) as leaders in STEM. Mentorship has emerged as a promising practice to address these challenges, yet a gap in the literature remains regarding the impact mentorship had on URM STEM leaders’ personal and professional development. This study responds to that gap by exploring the lived experiences of URM STEM leaders and their perceptions of how mentorship shaped their career development, psychosocial growth, and identity …

Online Parameter Adaptation Of Lqr Controllers Via Rls For Prosthetic Joint Control: Experimental Validation On A Quanser Qube-Servo 2 Platform, Cynthia Lopez-Jordan

Theses and Dissertations

This thesis develops and experimentally validates an online adaptive Linear Quadratic Regulator (LQR) control method for prosthetic joint systems using Recursive Least Squares (RLS)-based real-time parameter estimation on the Quanser QUBE-Servo 2 platform. Traditional LQR controllers assume a fxed system model, which limits adaptability and results in reduced tracking accuracy, poor robustness, and loss of optimal performance when applied to dynamically changing prosthetic joints, infuenced by load variations, user gait changes, and mechanical wear. Limited experimental validation exists for combining RLS with online LQR adaptation in prosthetic-like systems.

To address these limitations, an RLS-driven Adaptive LQR framework was implemented to …

Wondering And Wandering, Madeleine Herisson-Leplae

Theses and Dissertations

For my thesis, I have taken the concept of excavation and used it as a visual metaphor for my artistic process. Using my recurring motifs of a hole, ladder, and the gem, I break down the writing into three sections. First, the hole, a metaphor for entering an image; second, the ladder, process as investigation; and lastly, the gem, which represents wonder and delight. This work is influenced by childhood fantasies, video games, and believing in magic.

Critical Success Factors For U.S. Fintech Startups In The Post-Covid-19 Era: Applying The Entrepreneurial Ecosystem Framework, Wen Hua

Theses and Dissertations

The extradentary surge of financial technology (fintech) enterprises has fundamentally transformed contemporary financial markets, yet extant scholarly literature exhibits a notable absence of a unified and theoretically grounded framework for explicating fintech startups’ success (Hamza et al., 2025). This dissertation addresses this theoretical lacuna by synthesizing an integrative entrepreneurial ecosystem framework to illustrate the critical success factors (CSFs) that catalyze fintech venture performance (Conley, 2014). Drawing upon established theoretical foundations, this research employs a multi-method research design, and develops a comprehensive framework for fintech startup companies’ success. The methodological approach commenced with a systematic meta-analysis of theoretical paradigms, establishing a …

Purpose-Driven Curriculum For 9th-12th Grades For Adulthood Careers & Life Gratification, Destiny Krystle Chioma Nnadozie

Theses and Dissertations

A purpose-driven or purpose-based curriculum is not the standard required for high schools in the United States. Students in grades 9th-12th are educated based on a curriculum that covers mathematics, English, science, and social studies (De Roux & Riehl, 2022; Xiao et al., 2018). The unique individual purpose of each student, realized while in high school, is not considered a priority, leaving students to enter their adult careers lacking true career fulfillment due to this misalignment (Baroudi et al., 2017; S. Lyons et al., 2015; H. Wu, 2024).  Using a qualitative systematic review research design, this study aimed to explore …

The Influence Of Fictional Storytelling On The Student Experience In A Gamified Classroom, Arrash Jaffarzadeh

Theses and Dissertations

This exploratory case study evaluated how the use of gamification and game-based learning (GBL) with the added element of storytelling affected engagement and attitudes of children ages 9 to 13 as they participated in quests to complete their learning objectives.  Archived artifacts of a computer technology class in a northern California private school using the Applied Computer Technology Education (ACTE) framework taught to grade 4-8 provided the data for the study.  Each quest was comprised of a fictional story that tied to the lesson. These stories involved the three archetypes of the warrior (herald), healer (ally), and mage (trickster) on …

Adhd Black Girl Magic: A Narrative Inquiry Of The Lives Of Black Women With Attention Deficit Hyperactivity Disorder In Doctoral Programs, Talisha Tolliver-Haithcox

Theses and Dissertations

Black women with attention-deficit/hyperactivity disorder (ADHD) in doctoral programs have not been a topic of interest. Instead, the emphasis of ADHD research has been on children and adolescents, with studies involving higher education focused on undergraduate students, which has left a significant gap in the literature. This study uses narrative inquiry to explore the lived experiences of Black women with ADHD navigating doctoral programs to fill that gap in research and improve the lives of these Black women. Utilizing disability theory, a blend of disability theory and critical race theory called DisCrit, and Black feminist theory, this research examines how …

Student Success, Suicide Rates: A Correlational Study Of Pressures On, And Expectations For High School Students In The United States, Matthew Baham

Theses and Dissertations

Suicide among high school students has become a growing health crisis, with mental health issues such as anxiety, depression, and suicidal ideation increasing at an alarming rate. The purpose of this quantitative research is to explore the correlation between high school graduation requirements, pressures associated with college applications, and rising suicide rates among high school aged students in grades 9 through 12. With the increase in academic demands, standardized tests, extracurricular activities and a competitive college admissions process, has created an enormously high-pressure environment in high school education. These stressors are shown to have an intense impact on high school …

The Effect Of Administrative Leadership On Culturally Relevant Pedagogy In Secondary Education, Marianne Gomis

Theses and Dissertations

As the student population in American schools has become more racially and ethnically diverse, there is an urgent need to develop vital tools to accommodate the children of many cultures. By 2027, 44.7% of public-school students will be White, 15.2% Black, 29.2% Hispanic, 6.3% Asian/Pacific Islander, 0.9% American Indian/Pacific Islander, and 3.8% two or more races, according to the National Center for Education Statistics. (Digest of Education Statistics 2017, Snyder et al., 2019).

This study examines how administrators define and implement culturally responsive teaching (CRT), their roles in developing a positive perspective on CRT in classroom practices, and the leadership …

A Phenomenological Investigation Of School Spending And Academic Proficiency In A U.S. Charter High School, Jacques-Michel Endené

Theses and Dissertations

This study investigated the much-debated issues of school spending effectiveness, or lack thereof, set in a representational charter high school on the West Coast of the United States. The study employs a phenomenological approach, with a qualitative data analysis to examine the processes and outcomes of school spending policies and programs.

The research initially embarks on an exploration of the historical context surrounding the controversies born from a congressional policy initiative that ignited the firestorm, to understand the unfolding of this national debate. Examining diverse school leaders’ first-hand observers’ take, race-sensitive Academic Gap inklings, socioeconomic-based disparities, and comprehensive review processes, …

Exploring The Perceptions And Experiences Of School Building Leaders At Public Charter Schools And The Impact They Have On The Provision Of Physical Education In Charter School Settings, Nathaniel Bouie Jr.

Theses and Dissertations

This qualitative phenomenology study aimed to explore the perceptions of school principals regarding physical education (PE) and its impact on school programming. Semi-structured interviews were conducted with ten school principals in an urban charter school district in the United States. The interviews were transcribed, and data were analyzed using the phenomenological approach under the lens of teacher socialization.

The findings of the study revealed that physical education was perceived as an inferior subject compared to core subjects such as mathematics, science, and language arts. School leaders viewed PE as less important than other subjects and often reduced its instructional time …

Teacher Stress, Well-Being, And Adaptation To Innovation, Amanda Leanore Pelloth

Theses and Dissertations

Teachers face many sources of stress; however, a consistent source is prompted by the need to cope with change as districts adapt to shifting paradigms. This study intended to fill Rogers’ (2003) KAP gap and the failure of innovations by infusing the study of well-being and emotion into the narrative by incorporating the transactional relationship between technostress and implementation efforts into the technochange process. The study investigated teachers’ cognitive appraisals of technochange (primary appraisal) and their adaptational coping processes (secondary appraisal) to understand how implementation impacts teachers’ well-being and their adaptive processes. A parallel convergent mixed methods approach with an …

R&D Efficiency Of Drug Development At Universities, John Aylworth

Theses and Dissertations

Research and development (R&D) efficiency in the pharmaceutical industry is a measure of the relative value of new and novel products cast against the costs required to make them. For six decades, the R&D efficiency of large pharmaceutical companies has declined to critical levels. Most recently, nearly half of the companies studied were experiencing negative R&D efficiency, or products whose costs to bring to market outweigh the value they offer both commercially as well as to the health and well-being of society (Schuhmacher et al., 2023). One of the most prominent consequences of the inefficiencies plaguing the drug development industry …

Analyzing Students’ Long-Term Motivational And Emotional Effects On Achievement Performance Within And Across Diverse Socioeconomic Backgrounds, Hyejin Kim

Theses and Dissertations

This dissertation examines how students’ motivational and emotional factors influence their future educational and professional achievements within and across diverse socioeconomic status (SES) groups. This study leverages a quantitative longitudinal study methodology employing a publicly available sample of 16,197 U.S. students from 10th grade through age 26. The theoretical model of this study is grounded in the situated expectancy-value theory (SEVT; Eccles & Wigfield, 2024) and generalized control-value theory (CVT; Pekrun, 2024), encompassing five latent constructs: expectancies for academic success, subjective task values, assurance about academic success, assurance about career success, and SES-related achievement performance. To investigate the relationships between …

Exploring Perceptions Of College Completion Obstacles Among First-Year Students With Disabilities, James Roshone Evans

Theses and Dissertations

Students with special needs encounter significant challenges that affect their development, social mobility, and ability to achieve personal and professional goals. Despite efforts to improve post-secondary education outcomes, enrollment rates among students with learning disabilities remain below average. This study examined the experiences of first-year college students with mild to moderate cognitive impairments to gain a deeper understanding of the barriers to degree completion. It examines mandatory education, California's special education laws, social support systems, and the theoretical frameworks of Tinto's theory on retention, Wahlberg's theory of academic achievement, and Astin’s scholarly roles theory. Interviews with 25 students with disabilities …

Multiphysics Modeling Of Material Response To High-Intensity X-Ray And Laser Pulses: Heating, Ablation, And Plasma Expansion, Youssef Abouhussien

Theses and Dissertations

This dissertation presents a computational framework to investigate material response under high-intensity X-ray fluxes produced by an exo-atmospheric nuclear detonation and laser-material irradiations, with a focus on heating, ablation, and plasma expansion phenomena relevant to satellite vulnerability and high-energy-density environments. A hybrid Monte Carlo and Two-Temperature Model (MC-TTM) was developed to simulate X-ray and laser energy deposition and thermal relaxation in metals and semiconductors across a range of X-ray and laser pulse durations from femtoseconds to nanoseconds. Results demonstrate distinct thermal behavior between materials, with ablation thresholds and phase transitions captured in good agreement with experimental data.

In parallel, a …

Exploring The Stem Career Identity Development Of Black Women Across Their Lifespan, Veronica Hurd

Theses and Dissertations

Although STEM is the fastest-growing career sector, Black women are grossly underrepresented as they account for 2.5% of the workforce. Research highlights that this underrepresentation is due to racialized structures in K-12, postsecondary, and career settings that restrict Black girls’ and women’s STEM opportunities. While macrosystems such as hegemonic ideologies, attitudes, and social conditions shape Black girls’ and women’s opportunities in STEM, they continue to persist and achieve their career goals. To explore these barriers and Black women’s persistence in this industry, this study draws from the autobiographical memories of 10 Black women in the field or formerly in the …

Multitask Learning For Named Entity Recognition And Relationship Extraction, Adrienne D. Hembrick

Theses and Dissertations

Information Extraction (IE) is a fundamental task in Natural Language Processing (NLP), involving the identification of structured information from unstructured text. Two core components of IE—Named Entity Recognition (NER) and Relation Extraction (RE)—are widely used to extract key concepts and the relationships between them across various domains. However, the sequential dependency of RE on the output of NER makes it vulnerable to error propagation: inaccuracies in entity recognition can negatively affect downstream relation extraction.

To mitigate this issue, Multitask Learning (MTL) has been proposed as an approach that jointly models NER and RE, aiming to improve overall performance and reduce …

Synthesis Of Novel Schiff Bases And Their Metal Complexes, Ryan J. Lukkarinen

Theses and Dissertations

Presented in this thesis is the synthesis and characterization of multiple thiosemicarbazones and dithiocarbazates, as well as their corresponding metal complexes. Chapter 1 is an introductory chapter exploring the significance and potential applications of this work. Chapter 2 focuses on thiosemicarbazone synthesis and characterization. Chapter 3 describes the synthesis and characterization of dithiocarbazates. Chapter 4 details metal complex synthesis using the previously described ligands. Chapter 5 summarizes the conclusions of the research and future work that may stem from it.

Predictive Modeling For Healthcare Data Using Nonlinear Bayesian Methods, Prince Kofi Asare

Theses and Dissertations

Unplanned hospital readmissions represent a significant challenge for healthcare systems, contributing to substantial financial burdens and highlighting gaps in patient care coordination. In the U.S., approximately 20% of Medicare beneficiaries are readmitted within 30 days, costing billions annually. Social determinants of health, such as income, housing stability, and social support, account for up to 80% of health outcomes, yet their integration into predictive models remains underexplored. This study introduces a novel Bayesian framework for predicting 30-day readmission risk, combining Gaussian Process models with spike-and-slab priors and Bayesian Lasso regression with Laplace priors. Utilizing Markov Chain Monte Carlo methods, the approach …

Sulfate Sources In Groundwater From An Agricultural Area Central Illinois, Christabel I. Obi

Theses and Dissertations

Increasing sulfate (SO₄^2-) concentrations in the water environment, corresponding with increases in urbanization and industrialization, are a rising global concern. Over 250 mg/L, sulfate threatens human health and ecosystems. Elevated concentrations influence carbonate rock weathering, contributing to the evolution of the global carbon cycle. Knowledge of sulfate sources, whether natural or anthropogenic, is essential for understanding sulfate transport and fate in groundwater. This study investigates SO₄^2- origin and transport in groundwater in an area dominated by agricultural land use in Mclean County, Illinois. Specifically, we explored these questions: 1) Do sulfate concentrations change with groundwater …