Digital Commons Network^™

Knowledge, Attitudes, And Perceptions Of Nurse Practitioners About Antibiotic Stewardship, Robert M. Hamilton

Theses and Dissertations

Background: Antibiotic stewardship (ABS) is a set of strategies to optimize the use of antibiotics with the goal of reducing antibiotic resistance, improving patient outcomes and decreasing unnecessary costs. ABS affects all venues of patient care, including outpatient, inpatient, and long-term care. While many strategies for ABS exist and best practice continues to evolve, successful ABS programs utilize a multidisciplinary approach. Nurse practitioners (NPs) play an essential role in health care education and represent a valuable potential resource for ABS efforts. The purpose of this study is to describe the knowledge, attitudes, and perceptions of NPs towards ABS.Methods: A convenience …

To What Extent The World Health Organization's Policies Of Antimicrobial Resistance Are Implemented In Egyptian Hospitals., Amira Farouk Hussein

Theses and Dissertations

Antimicrobial resistance is a major public health problem. In the Egyptian hospitals, the problem was discovered since 2006; however, seven years ago, this problem drew the attention to be one of the major risk factors worldwide and in the Egyptian hospitals as well. International organizations, such as the World Health Organization (WHO) tried to find different solutions to prevent transmission of these communicable diseases from country to another by setting different guidelines and standards to be followed by countries. The case is to assess the barriers to implementation of objectives and guidelines of WHO in one of the biggest public …

State Requirements For Mandatory Vaccine Education Prior To Exemption, Chelsea Lynn Schult

Theses and Dissertations

Background: To prevent the spread of Vaccine Preventable Diseases (VPD’s), school-aged children in the United States are required to receive vaccinations. In certain states, parents have the option to exempt their child from becoming vaccinated due to medical, religious, or personal reasons. The purpose of this research was to identify which states require mandatory vaccination education prior to granting vaccine exemptions and to identify the various types of required vaccination education.Methods: Immunization program managers from states requiring parents seeking exemptions to receive mandatory vaccination education were asked a series of open-ended questions. The interviews were conducted via telephone. The education-related …

We Shall Overcome: The Association Between Family Of Origin Adversity, Coming To Terms, And Relationship Quality For African Americans, Kylee Marshall

Theses and Dissertations

Because adverse childhood and family of origin experiences may have implications for adult relationships, it is important to understand what can help buffer potential negative effects. The current study was designed to understand the relationship between childhood family of origin adversities, coming to terms with family of origin issues and events, and adult romantic relationship quality for African Americans (N = 1613). A path analysis was conducted using data from the RELATE assessment (see www.relate-institute.org). Results indicated that, taken together, coming to terms, self-esteem, and depression likely partially mediated the relationship between family of origin adversity and adult relationship quality. …

Influence Of Authority And Message Framing On Compliance With Mental Health Treatment Recommendations, Morgan A. Ess

Theses and Dissertations

The purpose of this study was to investigate the influence of source authority and message framing on compliance with mental health treatment recommendations. The current study used measures of attitudes and intentions to seek psychological help as well as the likelihood that an individual will request initial counseling information as proxies for observing help-seeking behavior. A pretest and posttest experimental design was implemented. Participants were 273 students at Illinois State University. At pretest, participants completed a demographic questionnaire, the Kessler K6+, the Mental Help Seeking Attitudes Scale (MHSAS), the Mental Help Seeking Intentions Scale (MHSIS), and indicated engagement in past …

Mississippi Mosquito Blood Meals And Haemosporidians, Jessica Aycock

Theses and Dissertations

Mosquitoes (Culicidae) transmit several parasites and pathogens including the causative agent of malaria, haemosporidians (Haemosporida). Transmission of these agents to and from the mosquito occurs during the collection of a blood meal. Because of this, it is imperative to gather data on current feeding patterns of mosquitoes. Prior to this study there were no published data on current feeding patterns of mosquitoes in Mississippi. Mosquitoes were captured with CDC light traps at eleven sites in two collection years. Engorged females were analyzed for blood meal content, and the vertebrate host was identified to species level in 72 mosquitoes. Previously published …

Observation Of Visitors At A Chimpanzee (Pan Troglodytes Schweinfurthii) Ecotourism Site Reveals Opportunity For Multiple Modes Of Pathogen Transmission, Darcey Glasser

Theses and Dissertations

Chimpanzee (Pan troglodytes) tracking is a popular ecotourism activity across Sub-Saharan Africa, offering visitors a personal wildlife experience. However, chimpanzee ecotourism may increase the risk of disease transmission between chimpanzees and people. This study assessed how tourist behaviors might facilitate cross-species disease transmission in Kibale National Park, Uganda.

Seen And Unseen: Visualizing Contradictions In Postwar Japan, 1950s–1960s, Christina Lai

Theses and Dissertations

This thesis offers a comparative study on how photography visualizes the political dynamics, ideological and psychological contradictions in postwar Japan. The discussion includes the exhibition The Family of Man in Tokyo (1956), Werner Bischof and Robert Capa’s photographs of Japan, and local photographers Ken Domon and Shomei Tomatsu.

Identification Of Inhibitors Of The Glutaminyl And Prolyl Trna Synthetases From Pseudomonas Aeruginosa, Yaritza Escamilla

Theses and Dissertations

Antibiotic resistance is a growing global threat and bacterial infections caused by antibiotic resistant bacteria have become increasingly difficult and expensive to treat with most current antibiotics. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen and a leading cause of nosocomial infections and is the leading cause of mortality in cystic fibrosis patients. Aminoacyl-tRNA synthetases (aaRSs) are a class of enzymes that catalyze the covalent attachment of amino acids to their cognate tRNAs during protein biosynthesis. The glutaminyl- tRNA synthetase from P. aeruginosa was over-expressed, enzymatically characterized and developed into a screening platform for the discovery of chemical compounds that inhibit …

The Development Of Screening Platforms To Identify Novel Anti-Infectives That Inhibit Protein Synthesis In Pseudomonas Aeruginosa, Casey Anne Hughes

Theses and Dissertations

Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is a leading cause of nosocomial infections and is becoming increasingly antibiotic resistant. Aminoacyl-tRNA synthetases (aaRSs) catalyze the covalent attachment of amino acids to their cognate tRNAs and serve as validated targets for the development of new anti-infectives. In P. aeruginosa, the genome contains two genes (tyrS and tyrZ) which encode two distinct TyrRS enzymes. The genes encoding both P. aeruginosa TyrRS-Z and TyrRS-S were cloned, overexpressed in E. coli cells, and purified to homogeneity. Both forms of TyrRS were active in aminoacylation and were developed into screening platforms using scintillation proximity …

Design And Synthesis Of Ceragenins–Cationic Steroid Antimicrobial Compounds, Structural Improvement And Synthesis Of Cyclopentenone Prostaglandins And Modification And Synthesis Of Derivatives Of Ribityllumazines: Potential Antigens For Activation Of Mait Cells, Yubo Li

Theses and Dissertations

Antimicrobial peptides (AMPs) are ubiquitous and display broad-spectrum antimicrobial activity that can control bacterial colonization of surfaces. Ceragenins are small-molecule mimics of AMPs and have several advantages over AMPs, including cost of manufacture and stability. A ceragenin, CSA-120, modified with an acrylamide group was directly incorporated into fluoropolymer coatings as a means of inhibiting bacterial biofilm formation. The ceragenin-containing coatings displayed improved performance. By conjugating a copper chelating group to the ceragenin, chelation of 64Cu by the conjugate was effective and provided a stable complex that allowed in vivo imaging. This conjugate may provide a means of identifying infection sites …

Regulation Of Immune Cell Activation And Functionby The Nbmpp2 Protein Andthe Cd5 Co-Receptor, Claudia Mercedes Freitas

Theses and Dissertations

According to the centers for disease control and prevention (CDC) and the world healthorganization (WHO), heart disease and immune related diseases such as diabetes and cancer areamong the leading causes of death around the world. Thus, the regulation of the function ofimmune cell plays a key role in health and disease. Calcium (Ca2+) ions play a critical role inimmune cell activation, function and in a robust immune response. Defects in Ca2+ signalinginfluences the development of cardiac disease, Alzheimer disease, immune cell metabolism,muscle dysfunction, and cancer. Each immune cell is unique in its activation and function,making it relevant to understand how …

Sustainable Polymers And Antimicrobial Biomaterials From Multicyclic Natural Products, Md Anisur Rahman

Theses and Dissertations

In this dissertation, the development of sustainable polymers and antimicrobial biomaterials from multicyclic natural products is illustrated. In Chapter 1, an overall background and recent development of sustainable polymers from natural product-based renewable biomass, antimicrobial biomaterials, and polymerization methods are introduced. Afterward, the primary research objectives of my doctoral research work are illustrated.

In chapter 2, designing block copolymer architectures toward tough bioplastics from renewable natural rosin was described. One of the most abundant natural biomasses is resin acids, however, most of the polymers derived from resin acids are brittle because of their bulky hydrophenanthrene pendant group. To overcome the …

The Intersections Of Health And Wealth: Socioeconomic Status, Frailty, And Mortality In Industrial England, Samantha Lee Yaussy

Theses and Dissertations

Socioeconomic status (SES) is considered one of the most powerful predictors of mortality today. However, studies of health in living populations and bioarchaeological studies of health in the past often oversimplify the connection between SES and mortality and overlook heterogeneity in frailty within a population and the potential for multiple types of marginalization to be layered within a single individual. This dissertation project uses skeletal samples to examine the interactions of SES, demographic characteristics (e.g., age and sex), exposure to physiological stressors, and mortality in the context of industrialization in 18th- and 19th-century England. Skeletal data from four industrial-era cemeteries …

Tool Migration: A Framework To Study The Cross-Disciplinary Use Of Mathematical Constructs In Science, Chia-Hua Lin

Theses and Dissertations

This dissertation is concerned with the scientific practice in which a mathematical construct that was originally developed to study a particular subject matter subsequently used in other disciplines or sub-disciplines for a different subject matter, a phenomenon that I call ‘tool migration.’ I argue that tool migration can be ‘epistemically risky.’ Specifically, uprooting a research tool from one disciplinary context and re-situating it for use in another can change how the tool is applied; whatever has made the tool useful and reliable in the first place may not have stayed the same in the new context. Using the migrations of …

Role Of Resources For Care In Improving Care Behaviors, Children’S Nutritional Status And Early Childhood Development In Low- And Middle-Income Countries, Sulochana Basnet

Theses and Dissertations

Poor child growth and suboptimal early childhood development are global health problems. Provision of appropriate care may help to achieve optimum child growth and development. Maternal resources for care such as education, knowledge, health, autonomy, reasonable workload, and social support may be needed to provide appropriate care behaviors, and improve children’s nutritional status, and early childhood development. The overall goal of this research was to understand the role of resources for care in improving care behaviors, child nutritional status, and early childhood development. The baseline Alive & Thrive household surveys from Bangladesh, Vietnam, and Ethiopia were used. We examined the …

Regression For Pooled Testing Data With Biomedical Applications, Juexin Lin

Theses and Dissertations

Since first introduced by Dorfman in 1943, pooled testing has been widely used as a cost and time effective testing protocol in the variety of applications. This dis- sertation consists of three projects that reveal the use of pooling techniques in the disease prevention from the perspective of regression. For disease monitoring and control, individual covariates information are often of practical interest and yield meaningful interpretations. It is natural to model the outcome of interest, which can be either a disease status (binary) or a biomarker concentration index (continuous), with individual-specific covariates through a regression analysis. Chapter 2 focuses on …

Ribosomally Synthesized And Post-Translationally Modified Peptides As Potential Scaffolds For Peptide Engineering, Devan Bursey

Theses and Dissertations

Peptides are small proteins that are crucial in many biological pathways such as antimicrobial defense, hormone signaling, and virulence. They often exhibit tight specificity for their targets and therefore have great therapeutic potential. Many peptide-based therapeutics are currently available, and the demand for this type of drug is expected to continue to increase. In order to satisfy the growing demand for peptide-based therapeutics, new engineering approaches to generate novel peptides should be developed. Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a group of peptides that have the potential to be effective scaffolds for in vivo peptide engineering projects. These …

Exploring Local Environmental Factors Influencing Geographic Distribution Of Black-Legged Tick Questing Activity, Chong Di

Theses and Dissertations

The deer tick (Ixodes scapularis or I. scapularis), also known as the black-legged tick, is the primary vector that transmits Lyme Disease (LD) in Northeastern United States. To contain the geographic expansion of Lyme disease ticks across the US in recent decades, ecological studies have been conducted to understand the biotic and abiotic environmental factors affecting tick activity. We observed in preliminary surveys that the tick host-seeking activity varies across small local areas. The primary objective of this project is to identify the environmental factors that impact deer tick questing activities at the micro-geographic scale. From 2017-2018, we …

A Bite Of The Big Apple: The Anthropology Of Pesticide Use In New York City, Faye O'Brien

Theses and Dissertations

Pesticide exposure in the developing world is well described in anthropology. How pesticide use and exposure is ordered and experienced socially, economically and culturally in Western urban communities is less well studied. The long-term consequences of synergistic pesticide exposure is not easily measurable, which this research addresses through social inquiry.

Somalia's Struggle To Integrate Traditional And Modern Governance: The 4.5 Formula And 2012 Provisional Constitution, Nasteha Ahmed

Theses and Dissertations

The objective of this project is to assess the impact of Somalia’s 4.5 power sharing formula on the consolidation of post-conflict governance. Per the peace agreement, seats in parliament are awarded to the four major clans which also dominate the election process. This has led to the perpetuation of many of the same social dynamics that lead to conflict in the past. Even though the 4.5 formula was not included in the constitution adopted in 2012, the elections methods from the formula were utilized in 2017 presidential elections. Although the goal is to move towards a one-man-one vote system, the …

Fabrication Of Electrospun Nanofibers Made Of Watermelon Peel Extract And Pva And Investigating Their Antioxidant And Antibacterial Activities, Wafaa Soliman

Theses and Dissertations

Food waste has become a huge problem globally since thousands of different food items are wasted all over the world which affects our environment severely due to the emissions of greenhouse gas due to anaerobic decomposition and leakage at the garbage. Fruit wastes like the peels and seeds are a major contribution to the waste products thrown everyday especially in juice industries. The antioxidant and antimicrobial activities of food wastes have been of great interest nowadays due to oxidative stress and antibiotic resistance problems. However the stability of the fruits or vegetables wastes was a challenge to perform these activities …

Changes In Self-Concept And Substance-Related Cognitions During Short-Term Residential Substance Use Treatment, Olivia Grace Larson

Theses and Dissertations

The United States is in the midst of an opioid crisis, with more than 130 people dying each day from an opioid overdose (Centers for Disease Control and Prevention [CDC], 2018a). Given the recent spike in overdose deaths associated with highly potent synthetic opioids (i.e., fentanyl), there are few signs of the crisis abating (CDC, 2018a). Compared to other age groups, the prevalence of both heroin and prescription opioid use is particularly elevated among young adults (Sharma, Bruner, Barnett, & Fishman, 2016). While there is a large body of evidence demonstrating the effectiveness of substance use treatment in general, only …

Development Of Bivalent Ligands Targeting The Putative Mu Opioid Receptor And Chemokine Receptor Cxcr4 Heterodimer, Bethany A. Reinecke

Theses and Dissertations

Human immunodeficiency virus (HIV) and opioid abuse have been described as synergistic epidemics. Pharmacologically, it has been found that opioids have the capacity to enhance HIV infection and replication. Research has shown that activation of the mu-opioid receptor (MOR) elevates the expression of the HIV-1 entry co-receptor CXCR4 on T-lymphocytes in the peripheral nervous system, thus allowing for enhanced viral entry and invasion. Although the exact mechanism for opioid modulation of CXCR4 expression and subsequent exacerbation of HIV is unknown, several hypotheses exist. One hypothesis is that MOR and CXCR4 are functionally interacting through the formation of a heterodimer. This …

Evaluating Predixcan’S Ability To Predict Differential Expression Between Alcoholics And Non-Alcoholics, John E. Drake Jr

Theses and Dissertations

PrediXcan is a recent software for the imputation of gene expression from genotype data alone. Using an overlapping set of transcriptome datasets from postmortem brain tissues of donors with alcohol use disorder and neurotypical controls, which were generated by two different platforms (e.g., Arraystar and Affymetrix), and an additional unrelated transcriptome dataset from lung tissue, we sought to evaluate PrediXcan’s ability to impute gene expression and identify differentially expressed genes. From the Arraystar platform, 1.3% of matched genes between the measured and imputed expression had a Pearson correlation ≥ 0.5. Our attempt to replicate this finding using the expression data …

Effects Of A Heroin Conjugate Vaccine On The Antinociceptive And Abuse-Related Effects Of Heroin In Rats And Monkeys, Kathryn L. Schwienteck

Theses and Dissertations

The increase in heroin use is one factor contributing to the current opioid epidemic in the United States. There are three Food and Drug Administration (FDA) approved medications for the treatment of opioid use disorder (OUD), and these include agonist (i.e. methadone and buprenorphine) and antagonist (i.e. naltrexone) therapies. Although these medications are effective for some patients, regulatory constraints for agonist therapies limit access and patient compliance for naltrexone is poor. The development of new therapies, such as immunopharmacotherapies, for the treatment of OUD is a priority for the National Institute of Drug Abuse. A heroin immunopharmacotherapy, or vaccine, produces …

Genetic And Environmental Factors Influence Drosophila Ethanol Sedation, Rebecca E. Schmitt

Theses and Dissertations

Alcohol use disorder is a global health issue that affects a significant portion of the population, with affects including both negative mental and physical consequences. Currently, there are few treatment options available to those who suffer from alcohol use disorder, alcohol abuse, or alcohol dependence. Identifying candidate genes or environmental influences would therefore improve the means for possible treatments or identification of those people at risk for alcohol use disorder. Previous studies in humans have demonstrated an inverse association between initial sensitivity and risk for alcohol abuse. This connection allows investigators, and our laboratory, to investigate genetic and environmental factors …

National And Local Antibiotic Prescribing Trends And Prescribing Appropriateness In Older Adults, Fawaz M. Alotaibi

Theses and Dissertations

Background: Antibiotic overuse/misuse has been documented in several reports to increase the risk of Clostridioides difficile (C.diff) infection and antibiotic resistance. The older adult population is more prone to use antibiotic medications than any other age group due to decreased immune function, use of urinary catheters, ventilation during hospitalization and other factors. Antibiotic resistance and C.diff are major public health problems. However, studies examining the trends of antibiotic use and the association between the antibiotic use and negative health outcomes among older adults in the outpatient and emergency department settings are limited.

Objectives: The main objectives of this …

Regulatory T Cells Promote Breast Cancer Progression Through Inhibiting Classical Activation Of Macrophages, Nicholas M. Clark

Theses and Dissertations

Transient ablation of regulatory T cells has been shown to be effective at hindering tumor growth and metastasis in murine breast cancer model. Based on our lab’s previous work, we have demonstrated that NK cells and CD8+ cytotoxic T cells were not required for the protective effect of Treg cell ablation. However, we also reported that CD4+ helper T cells and IFN-γ were required for the protective effect of Treg cell ablation. Furthermore, we observed that CD11B+ cells responded to Treg ablation therapy by up-regulating target genes of IFN-γ. Therefore, this study aimed to investigate the connection between the myeloid …

Fluvastatin And Microrna-146a Alter Interleukin-33 Mediated Mast Cell Functions., Marcela Taruselli

Theses and Dissertations

Mast cells are tissue-resident immune cells known as effector cells for the innate and adaptive immune systems. Mast cells contribute to host defenses against parasites such as large roundworm parasites, bacterial pathogens, and toxins, and participate in wound healing, but they are mostly known for their role in allergic diseases. It has been well established that during allergic diseases, mast cells are stimulated by IgE cross-linkage to release proinflammatory mediators. However, a newly discovered cytokine, IL-33 has also been implicated in allergic disease. Recently, IL-33 has been implicated as a driver of several Type I sensitivities and previous studies have …