Digital Commons Network^™

Investigating The Reversibility Of Protein Adsorption On Gold Nanoparticles And The Role Of Free Thiol, Awosiji Olatunde Awotunde

Theses and Dissertations

Protein-gold nanoparticle (AuNP) bioconjugates have many potential applications in nanomedicine. A thorough understanding of the interaction between the protein and AuNP is critical to engineering these functional bioconjugates with desirable properties. In this work, we investigate the role of free thiols presented by the protein on the stability of the protein-AuNP conjugate. Human serum albumin (HSA) was modified with 2-iminothiolane (Traut’s reagent) to introduce additional thiols on the protein surface, and three variants of HSA were synthesized to present 1, 5, and 20 free thiols by controlling the molar excess of the chemical modifier. Protein exchange studies on AuNPs were …

Synthesizing Modified Polymeric Nanoparticles For Biofilm Inhibition, Antibiotic Encapsulation, And Specific Targeting Against Pseudomonas Aeruginosa, Logan Schnorbus

Theses and Dissertations

Widespread usage of antibiotics is a growing concern due to antibiotic resistance development in bacteria. This is due to common use of antibiotics in agricultural, livestock, and clinical usage. Antibiotic resistance is developing at a rate in which it is outpacing new drugs on the market. New strategies in drug development are necessary to combat the increasing resistance. We designed several motifs of sugar-modified nanoparticles to inhibit the biofilm formation of Pseudomonas aeruginosa.

P. aeruginosa is an opportunistic pathogenic bacterium that is responsible for common life-threatening infection in hospitals. This gram-negative opportunistic pathogenic bacterium infects hosts with compromised immune systems …

A Systems-Level Approach To The Design, Evaluation, And Optimization Of Electrified Transportation Networks Using Agent-Based Modeling, Landon Clark Willey

Theses and Dissertations

Rising concerns related to the effects of traffic congestion have led to the search for alternative transportation solutions. Advances in battery technology have resulted in an increase of electric vehicles (EVs), which serve to reduce the impact of many of the negative consequences of congestion, including pollution and the cost of wasted fuel. Furthermore, the energy-efficiency and quiet operation of electric motors have made feasible concepts such as Urban Air Mobility (UAM), in which electric aircraft transport passengers in dense urban areas prone to severe traffic slowdowns. Electrified transportation may be the solution needed to combat urban gridlock, but many …

A Patient-Specific Adaptation Of The Living Human Heart Model In Application To Pulmonary Hypertension, Yousof Abdel-Raouf

Theses and Dissertations

The Living Heart Project aims to offer medical practitioners and researchers a full-heart electromechanical computational platform to explore and assess clinical cases pertaining to the left ventricle (LV), and the less addressed right ventricle (RV). It does not, however, provide an easy solution to applying this platform to patient-specific cases that account for a large variability among cases. We, therefore, present a solution to modify the Living Human Heart Model (LHHM) to obtain a patient-specific geometry using the thermal expansion method, with iteratively adjusted parameters that accurately simulate the case of a 72-year-old female patient suffering from secondary pulmonary hypertension …

Isothermal Inactivation Of Salmonella, Listeria Monocytogenes, And Enterococcus Faecium Nrrl-B 2354 In Peanut Butter, Powder Infant Formula, And Wheat Flour, Adam Robert Quinn

Theses and Dissertations

Pathogens in low-moisture foods are an emerging food safety concern due to increased survival and thermotolerance in matrices with low water activity. However, limited data is publicly available for the thermotolerance of Listeria monocytogenes, Salmonella spp., and Enterococcus faecium NRRL B-2354 (a Salmonella surrogate). The aims of this study were to identify differences in thermal inactivation rates between these organisms in three different low-moisture foods. Three model low-moisture foods (peanut butter, powder infant formula, and wheat flour) were inoculated with either E. faecium, a Salmonella spp. cocktail, or a L. monocytogenes cocktail using a dry inoculation method for a total …

The Effects Of Immune Regulation And Dysregulation: Helper T Cell Receptor Affinity, Systemic Lupus Erythematosus And Cancer Risk, And Vaccine Hesitancy, Deborah K. Johnson

Theses and Dissertations

Helper T cells direct the immunological response to foreign pathogens and cancer. To become activated, helper T cells must recognize unique peptides presented on major histocompatibility complex II (pMHCII) by antigen presenting cells (APCs) with their T cell receptor (TCR). While much is known about helper T cell activation signaling cascades and the subsequent roles of helper T cell subsets, the initiation of helper T cell activation by the TCR and other co-receptors is less well understood. Specifically, the affinity of the TCR for its pMHCII can change helper T cell subset fate, proliferation, and alter the risk for activation …

Homeless In Little Rock: An Exploration Of Best Practices For Solving Homelessness, Leonardo Victor Moya

Theses and Dissertations

This Best Practice Manual attempts to provide answers to the question: How can government and communities learn from one-another in creating a plan that would make homelessness for the (at risk) homeless population a thing of the past?

Synthesis Of Functionalized Streptococcus Pneumoniae Serotype 6a Di- And Tri- Saccharides, Brady Davis James

Theses and Dissertations

There is a rise in prevalence of antibiotic resistance in Streptococcus pneumoniae, and its FDA-approved vaccines often do not mount effective immune responses in children, the elderly, or the immunocompromised. One reason these vaccines are generally less effective is because they do not utilize T-cell help. T-cell help can be accessed when di-, tri-, or tetra-saccharides positioned inside major histocompatibility complex (MHC) II are presented to T-cell receptors as a target antigen. Pairing MHC II-antigen complexes with T-cell receptors enables development of B and T lymphocytes that are highly specific to these antigens, granting an increase in antibody affinity and …

Prediction Of Drug-Drug Interaction Potential Using Machine Learning Approaches, Joseph Scavetta

Theses and Dissertations

Drug discovery is a long, expensive, and complex, yet crucial process for the benefit of society. Selecting potential drug candidates requires an understanding of how well a compound will perform at its task, and more importantly, how safe the compound will act in patients. A key safety insight is understanding a molecule's potential for drug-drug interactions. The metabolism of many drugs is mediated by members of the cytochrome P450 superfamily, notably, the CYP3A4 enzyme. Inhibition of these enzymes can alter the bioavailability of other drugs, potentially increasing their levels to toxic amounts. Four models were developed to predict CYP3A4 inhibition: …

Numerical Simulation To The Fitzhugh-Nagumo Model With Strong Reaction, Hongsong Feng

Theses and Dissertations

The Fitzhugh-Nagumo model is a mathematical model derived from the simulation of propagating pulses in multicellular organisms. Since its creation, this model has drawn great attentions from academics and industry. To better understand the properties underlying this system, suitable numerical methods are needed to study it. In this thesis, numerical methods including the finite difference method, the finite element method, and the least-squares finite element method are applied to approximate its traveling wave solutions. In particular, since the FitzHugh-Nagumo model with strong reaction has a significant role in application, appropriate numerical scheme is designed to study it. Consistency and stability …

Biophysical Characterization Of Protein-Nanoparticle Interactions, Yasiru Randika Perera

Theses and Dissertations

Nanoparticles (NPs) have become a key tool in medicine and biotechnology; as drug delivery systems, biosensors, and diagnostic devices. However, the mechanism of biocorona formation on nanoparticle surfaces and their impact on drug delivery remains speculative. Nevertheless, functionalized nanoparticles have demonstrated major success in medical applications; having been shown to effectively treat disease. The mechanistic details of protein behavior on nanoparticle surfaces remain poorly understood to date; due to difficulty in determining the orientation and structure of protein on NPs. Furthermore, surface crowding, orientation, and degree of disorder have been shown to perturb the efficacy of protein on NPs; dramatically …

Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah

Theses and Dissertations

American oyster (Crassostrea virginica) is a popular seafood for its delicacy and high nutritional value. Based on increasing concern about contamination of bacterial pathogens in raw oyster, my research objectives have been focused on detection and enumeration of two important bacterial pathogens, Escherichia coli and Salmonella spp. in the American oyster in south Texas waters, local markets and controlled laboratory studies. Immunohistochemical and RT-PCR analyses showed substantial bacterial pathogen’s presence in gills and digestive glands of oysters collected from San Martin Lake and South Padre Island as well as local markets. Laboratory studies showed increasing trend of both …

Physiological Consequences Of Adverse Early-Life Experiences: A Skeletal Investigation Of Frailty And Resilience Within An Institutionalized Sample Using A Modified Version Of The Skeletal Frailty Index (Sfi), Ashley Dafoe

Theses and Dissertations

This study investigates frailty, defined as the accumulation of deficits in physiological functioning, by applying the Skeletal Frailty Index (SFI) to a skeletal sample (N=67) recovered from the Mississippi State Asylum (MSA), and in a comparative sample, the Terry Collection. The SFI was statistically modified to increase its utility here. Variables that influence frailty, including age, sex, stress in early-life, and resilience, were assessed relative to four SFIs: Overall, Nutritional, Activity, and Infection. This study finds that the predicted relationships between the SFIs and the aforementioned variables are largely absent in the MSA sample. When compared to individuals in the …

Preliminary Investigations Into Pharmacodynamic Monitoring Of Cyclosporine In Cats, Harry Cridge

Theses and Dissertations

Existing pharmacokinetic monitoring tools for cyclosporine fail to correlate with clinical response. In dogs, pharmacodynamic monitoring of nuclear factor of activated T cell (NFAT) regulated cytokines is thought to provide a better overall evaluation of the immune response to cyclosporine than blood levels; however, such monitoring tools are not available in cats. In this study, we designed and optimized a protocol for maximal T lymphocyte stimulation in cats. This is the first step in the development of a pharmacodynamic monitoring tool for cyclosporine in cats based on expression of NFAT-regulated cytokines. We also confirmed that cyclosporine has anti-lymphocytic properties in …

Metagenomic Sequencing, Microbiome Reconstruction, And Analysis Of Ancient North And Central American Dental Calculus, Jonathan Belanich

Theses and Dissertations

Analyses of human oral microbiomes reveal substantial amounts of information about health, diet, and diseases of individuals and their communities within the archaeological record. In order to examine bacterial genomes from the past, specific archaeological samples that contain remnants of the microbial communities in question must be utilized. Recent developments in high-throughput, next-generation DNA sequencing have enabled the characterization of entire oral microbiomes and genomes from the remains of the bacteria trapped in calcified dental plaque. This project analyzed samples of ancient human dental calculus from North and Central America, which were examined for the changes within the oral microbiome …

A Comparison Of Chikungunya Virus Infection, Dissemination, And Cytokine Induction In Human And Murine Macrophages And Characterization Of Rag2-/-Γc-/- Mice As An Animal Model To Study Neurotropic Chikungunya Disease, Israel Guerrero

Theses and Dissertations

Chikungunya virus (CHIKV) is classified as an alphavirus in the Togaviridae family. This virus is known to rely on Aedes arthropod vectors for its dissemination. Human infection is characterized by rash, high fever, and severe chronic polyarthritis that can last for years. Recently, efforts in developing animal models have been made in an attempt to better understand CHIKV pathogenesis. CHIKV infection starts with a 7 to 10 day long febrile acute phase, in which most of the symptoms occur (rash, fever, and incapacitating pain in joints and muscle). Once the immune system clears most of the viral infection, a chronic …

Three-Dimensional Hydrodynamic Focusing For Integrated Optofluidic Detection Enhancement, Erik Scott Hamilton

Theses and Dissertations

The rise of superbugs, including antibiotic-resistant bacteria, and virus outbreaks, such as the recent coronavirus scare, illustrate the need for rapid detection of disease pathogens. Widespread availability of rapid disease identification would facilitate outbreak prevention and specific treatment. The ARROW biosensor microchip can directly detect single molecules through fluorescence-based optofluidic interrogation. The nature of the microfluidic channels found on optofluidic sensor platforms sets some of the ultimate sensitivity and accuracy limits and can result in false negative test results. Yet higher sensitivity and specificity is desired through hydrodynamic focusing. Novel 3D hydrodynamic focusing designs were developed and implemented on the …

Kujdesi Infermieror Në Menaxhimin E Infeksioneve Intrahospitalore, Sidorela Pulaj

Theses and Dissertations

Infeksionet intrahospitalore (brenda spitalit) ose të quajtura ndryshe infeksione nozokomiale përkufizohen si infeksione që nuk kanë qënë të pranishme apo nuk janë në fazën e inkubimit në momentin e pranimit te pacientit në spitale. Termi nozokomial ka origjinë nga greqishtja nozos (sëmundje) dhe komein (përkujdesje) dhe nënkupton infeksionet që janë marrë si rrjed- hojë e përkujdesjes mjekësore. Infeksionet intrahospitalore janë të gjitha infeksionet që mer- ren 48 orë deri në 72 orë pas shtrimit të pacjentit në spital, pra infeksionet spitalore janë marrja e një infeksioni tjetër plus gjatë qëndrimit në spital. (Misini, et.al, 2012).

Qëllimi i këtijë punimi ka …

Njohuritë E Prindërve Rreth Vaksinimit Të Fëmijëve Kundër Sëmundjeve Ngjitëse, Xheneta Shabani

Theses and Dissertations

Duke qenë se shëndeti është gjëja kryesore për të gjithë, sidomos për ne si profesionistë shëndetësor, duhet që ne çdo hap që e bëjmë drejt ruajtjes dhe përmirësimit të tij. Si infermierë të rinjë, kjo është ndër detyrat tona primare që mund ta arrijmë përmes punës dhe edukimit tonë. Në temën time kam trajtuar njohuritë e prindërve rreth vaksinimit të fëmijëve kundër sëmundjeve ngjitëse, pikërisht temë e cila ka zgjuar shumë interes te prindërit e rinjë. Duke qenë se rëndësia e vaksinimit është mjaft e madhe tek një popullatë për të qenë sa më e shëndetshme, është e nevojshme që …

Social Media Posts' Influence On College Athletes' Protein Knowledge, Kriston T. Love

Theses and Dissertations

This research aimed to examine how Instagram can be used as a means of education for collegiate athletes and their knowledge of protein. More specifically this research aimed to compare the prior knowledge about protein that collegiate athletes have to the knowledge they may have retained after being exposed to posts on Instagram about protein. It also aimed to determine the knowledge of protein collegiate athletes already had and if their sport and year in school influenced their protein knowledge. This study surveyed Division I collegiate athletes on their social media use and knowledge of protein before and after being …

Checkmate: Exploring Father-Son Communication Regarding Reproduction And Sexual Health In Males With Cystic Fibrosis, Dianna C. Sanderson

Theses and Dissertations

Cystic fibrosis (CF) is a heritable monogenic condition with allelic heterogeneity. A variety of sequence alterations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene results clinically in multisystem disease including a reduced reproductive capacity in affected males. Knowledge of reproductive capacity is often disclosed to parents by medical providers and to affected male children by medical providers and their parents. Despite advanced technology and therapeutics yielding the benefits of increased life expectancy and quality of life, males affected with CF largely remain uninformed in broader areas of sexual health and particularly in how their concept of masculine …

Development Of Natural Product-Derived Cationic Polymers For Biomaterial Applications, Moumita Sharmin Jui

Theses and Dissertations

In this thesis, multicyclic natural products and metallocene-containing polymers were synthesized for biomedical applications.

Chapter 1 describes the overall background, the recent development of natural product-based antimicrobial biomaterial and metallocene containing polymer for biomedical application. Afterward, the primary research objectives of my research are illustrated.

In chapter 2, a multicyclic natural product derived guanidine-based facially amphiphilic homopolymers and copolymers were synthesized. Cholic acid is one of the bile acid derivatives used to prepare facially amphiphilic moieties. A neutral polyethylene glycol (PEG) component was used to prepare copolymers with cholic acid. Reversible-addition fragmentation chain transfer (RAFT) polymerization technique was used to …

Structural, Functional And Immunological Studies Of Group 1 And 2 House Dust Mite And Profilin Allergens For The Development Of Allergen Specific Diagnostics And Immunotherapy, Anyway B. Kapingidza

Theses and Dissertations

Group 1 and 2 house dust mites (HDM) allergens elicit IgE response in more than 80% of sensitized individuals. These allergens affect 10-30% of the world’s population. Prolonged inhalation of low doses of allergens (microgram amounts inhaled over months or years) by genetically predisposed individuals leads to the induction of a Th2-type immune response induced by allergen-specific immunoglobulin E (IgE) produced by plasma cells resulting in allergic diseases like asthma, atopic dermatitis, and rhinitis. Group 1 and 2 HDM allergens, in particular, are associated with the development of the aforementioned allergic diseases. The goal of this research was to characterize …

The Impact Of Psychosocial, Clinical, And Neighborhood Factors On Inflammatory Outcomes Among African American Women, Malcolm Seth Bevel

Theses and Dissertations

Inflammatory-related chronic diseases are disparagingly prevalent among African Americans in the United States, particularly in the Southeastern region. Access to community gardens shows promise in mitigating chronic disease outcomes, despite multiple barriers to physical activity and diet among underserved populations. We seek to determine if psychosocial or clinical factors mediate the association between the treatment effect of a healthy lifestyle intervention and inflammatory biomarker levels among African American women, respectively. We also seek to analyze the relationship between residing in a food desert/swamp and inflammatory biomarker levels, while gaining a qualitative perspective about community garden access among African American women. …

Experiences Of College Students In Addiction Recovery: A Critical Case Study, Dory E. Hoffman

Theses and Dissertations

The purpose of this study is to investigate the lived experiences of college students in addiction recovery. Critical ethnographic case study was used to challenge existing paradigms and educational practices regarding students in recovery. The lived experiences of this population are described to share their stories and work towards better policy solutions to the U.S. college drug epidemic.

Development, Validation, And Application Of A Novel Method To Separate And Quantify Silver Intracellular Uptake In Pseudomonas Aeruginosa, Mohammed A. Othman

Theses and Dissertations

Silver nanoparticles (AgNPs) have attracted tremendous attention as a potential broadspectrum antimicrobial agent to overcome multidrug resistant (MDR) infections. However, a comprehensive understanding to AgNPs bactericidal mechanism of action and the relative role of particulate versus ionic Ag in AgNPs antibacterial activity is lacking but essential for their optimization for potential medical applications. Therefore, a novel method to separate and quantify Ag internalization in P. aeruginosa was developed and validated through multimethod approach. The methods used were optical density at 600 nm (OD600), LIVE/DEAD staining, transmission electron microscopy (TEM), and sorbed Ag liberation assays. After optimizing the method, it was …

Surveying Tuberculosis Screening Practices Of South Carolina Hospital Employees, Tamara T. Carree

Theses and Dissertations

BACKGROUND: Every year tuberculosis (TB) causes over 10 millions of people globally and in recent years has caused over 9,000 cases annually in the United States. TB can be transmitted in hospital settings, and South Carolina hospitals, like hospitals elsewhere in the country maintain a responsibility to test their employees for this disease. Nosocomial infections of active TB among patients and healthcare workers can be burdensome on hospital resources. Testing healthcare workers and implementing prevention strategies are examples of administrative controls provided in the 2005 guidelines by the Centers for Disease Control and Prevention (CDC). Implementation of administrative controls are …

The Effects Of Racialization On Skeletal Manifestations Of Disease Among Migrants In Historic St. Louis, Missouri, Kristina M. Zarenko

Theses and Dissertations

This research investigates the biological effects of racialization on migrants in late nineteenth and early twentieth century St. Louis, Missouri. Racialization is a form of structural violence in which real or perceived physical differences contribute to the creation of hierarchical racial categories along a continuum of whiteness. German and Irish immigrants and African American migrants from the South came to St. Louis in search of economic prosperity and in an attempt to escape poverty, famine, or conflict in their places of origin. However, racialization affected each migrant group’s access to housing and employment as well as their exposure to violence. …

School Nurses' Role During Disease Outbreak, Christina Rickenbach

Theses and Dissertations

Background and Purpose: School nurses have the critical task of overseeing the health and wellness of school-aged students. Utah is 1 of 17 states which allow parents to exempt their children from vaccines. The most common parental reason for exempting children from vaccines is personal choice. With the number of students without vaccinations on the rise, school nurses are tasked with responsibilities related to the spread of vaccine-preventable diseases. However, few studies have explored the role of school nurses in managing a disease outbreak. Therefore, the purpose of this study was to 1) explore the responsibilities of Utah school nurses …

Roli I Infermierit Në Trajtimin E Pacientëve Nga Abcesi I Trurit, Mollëza Kamberi

Theses and Dissertations

Abcesi i trurit (BA) përcaktohet si një infeksion fokal brenda parenkimës së trurit, e cila fillon si një zonë e lokalizuar e cerebritit, e cila më pas shndërrohet në një koleksion qelbi brenda një kapsulë të vaskuluar mirë. BA duhet të jetë e diferencuar nga infeksionet duke përfshirë abscesin epidural dhe subdural. BA është një sfidë për neurokirurgun sepse kërkon aftësi të mira klinike, farmakologjike dhe kirurgjike për të siguruar rezultate të mira klinike dhe prognozë për pacientët me BA. E konsideruar si një infeksion i rrallë i trurit, BA mund të ishte një entitet shkatërrues që e la me …