Digital Commons Network^™

Oxygenation Of The Root Zone And Tce Remediation: A Plant Model Of Rhizosphere Dynamics, Ian F. Thompson

Theses and Dissertations

This study analyzes rhizosphere conditions that enhance the effective aerobic degradation of TCE in wetland bioremediation systems. A plant model was built using Stella 9.0 modeling software and uses numerical integration evaluation; it addresses movement of oxygen through plant vascular and aerenchymal systems, and into the rhizosphere where oxygen and other substrates influence bacteria. Methanotrophs and heterotrophs are assumed to be influential bacteria groups. Variations of humidity-induced-convection, methane, soil carbon, and copper concentrations are evaluated. Varying concentrations and hydraulic loadings of TCE are assessed with respect to TCE consumption rate and TCE treatment efficiency. Soil conditions most directly affected TCE …

Investigation Of Inosine And Hypoxanthine As Biomarkers Of Cardiac Ischemia In Plasma Of Non-Traumatic Chest Pain Patients And A Rapid Analytical System For Assessment, Don E. Farthing

Theses and Dissertations

Each year in the U.S., approximately 7-8 million patients with non-traumatic chest pain visit hospital emergency departments (ED) for medical evaluation. It is estimated that approximately 2-5% of these patients are experiencing acute cardiac ischemia, but due to the shortcomings of current test methods, they are incorrectly diagnosed and discharged without appropriate treatment provided, thus leading to poor patient outcome and potential medical malpractice litigation.The goals of this research were to evaluate plasma samples for potential biomarker(s) of acute cardiac ischemia prior to heart tissue necrosis, and to ultimately develop a rapid method for detection of the potential biomarker(s) in …

Analytical Modeling With Decoupled Optimal Control Of Biomechanical Sit -To -Stand Movement, Asif Mahmood Mughal

Theses and Dissertations

Biomechanical movements with multiple degrees of freedom exhibit complex coordination and control mechanisms in the presence of physiological constraints. A human body biomechanical model which can mimic diverse 3D physiological movements is a challenging task. In this study, we develop a mathematical framework to study human sit-to-stand (STS) movement using 2D biomechanical models. This analytical framework models the physiological system for feedback controller design. A 2D 4-segment biomechanical model with optimal feedback controller design provides a motivation to extend this scheme for analytical 3D bipedal modeling. We used a newly developed mathematical modeling toolbox (Maple’s DynaFlexPro) to generate 3D 8-segment …

Role Of Vegf-C In Proliferation And Migration In A Cancer Model, Emily Marie Benke

Theses and Dissertations

Head and neck cancer ranks high among the most common cancers world wide. In addition, there is a high recurrence rate, as well as a high prevalence of loco-regional tumor spread. Among many factors contributing to metastasis is vascular endothelial cell growth factor C. VEGF-C is primarily an inducer of new lymph vessel formation, typically during embryogenesis; however, some advanced cancers show a significant increase in VEGF-C expression, suggesting a role in metastasis. In the current study, the effects of VEGF-C expression were tested in HN12 cells, which are highly metastatic and known to express high levels of the chemokine …

Molecular Characterization Of Dopamine Transporter Phosphorylation And Regulation, Balachandra Kumar Gorentla

Theses and Dissertations

The dopamine transporter (DAT) is a presynaptic membrane phosphoprotein that terminates dopaminergic synaptic transmission by clearing dopamine (DA) back into presynaptic neuron by a reuptake process. DAT is also a molecular target for psychostimulants such as cocaine, amphetamine (AMPH) and methamphetamine (METH) that increase DA synaptic levels either by blocking reuptake or by inducing DA efflux. DAT phosphorylation and transport down regulation are best demonstrated with the treatment of Protein kinase C (PKC) activators such as (phorbol 12-myristate 13-acetate) PMA. Serine(s) and threonine(s) are known to be involved in DAT phosphorylation.

Pretreatment with psychostimulant substrates such as AMPH and METH …

Identification Of Psychological Stressors For Long Duration Space Missions: Psychological Stressors Among Five Astronauts And Cosmonauts, Melinda S. Marsh

Theses and Dissertations

As humans continue to experience space missions of longer and longer duration, more and more high intensity stressors are going to be experienced. Although physiological stressors have been studied, there have been few studies on the psychological stressors. Those that are present primarily focused on the polar environment as a space analogue. In order to answer the question of how stressors may affect future astronauts and cosmonauts, the evolutoinary reasons behind stress must be considered not only the type and intensity of the stressors.

Five astronauts and cosmonauts representing three space agencies were surveyed regarding their experiences with ten types …

Amp-Activated Protein Kinase Kinase Activity And Phosphorylation Of Amp-Activated Protein Kinase In Contracting Muscle Of Sedentary And Endurance Trained Rats, Denise Hurst

Theses and Dissertations

This study was designed to examine activity of AMP-activated protein kinase kinase (AMPKK) and AMP-activated protein kinase (AMPK) in muscles from control (C) and endurance trained (T) rats. Rats were trained 5 days/wk, 2 hr/d for 8 wks at a final intensity of 32 m/min up a 15% grade with 30 second sprints at 52 m/min every 10 min. Gastrocnemius muscles were stimulated in situ in T and C rats for 5 min at frequencies of 0.4/sec and 1/sec. Gastrocnemius LKB1 protein, a putative component of the AMPKK complex (LKB1, STRAD, and MO25), increased approximately 2-fold in response to training. …

Phosphorylation And Regulation Of The Dopamine Transporter By Psychostimulant Substrates And Protein -Protein Interactions, Mark A. Cervinski

Theses and Dissertations

The dopamine transporter (DAT) is an integral membrane protein that clears dopamine (DA) from the synaptic space following neurotransmission. DATs amino acid sequence contains numerous serine, threonine and tyrosine residues, some of which are located in protein kinase consensus sites. While DAT phosphorylation and regulation in response to protein kinase C activation is well documented, evidence suggests that multiple signals and interacting proteins also mediate these processes. Previous research has also demonstrated that DAT regulation is influenced by the presence of the DAT substrates amphetamine (AMPH), methamphetamine (METH) and the uptake blocker cocaine. Using 32PO4 metabolic labeling and [3H]DA uptake …

Exploring The Ligand Binding Site On The Dopamine Transporter By Photoaffinity Labeling And Site-Directed Mutagenesis, Maria Laura Parnas

Theses and Dissertations

The dopamine transporter (DAT) is a neuronal presynaptic transmembrane protein that clears released dopamine (DA) from the synaptic space, regulating the neurotransmitter concentration and availability, DAT and the related serotonin (SERT) and norepinephrine (NET) transporters belong to the SLC6 family of Na+ and CI- dependent symporters, and are major targets for the action of several drugs, including the psychostimulant cocaine. DAT is predicted to possess 12 transmembrane spanning domains (TMs), with both N- and C-termini located intracellularly. Extensive research efforts to gain insight on the molecular aspects of DAT have been attempted, but the three-dimensional arrangement of the protein as …

Studies On Cyclooxygenase-1, Its Structure And Splice Variants, And Modulation Of Cyclooxygenase-2 By Inducible Nitric Oxide Synthase And Novel Phytochemicals., Yibing Xu

Theses and Dissertations

Cyclooxygenases (COXs) are of important therapeutic value as they are the target site of aspirin-like drugs. Here I report nine new COX-1 splice variants in chapter 1, which I characterized with regard to heme-binding and other properties. Inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) are co-inducible in many tissues following mitogenic and proinflammatory stimulation. In chapter 2, I investigate the physical and enzymatic properties of human COX-2 and iNOS and demonstrate that, despite reports to the contrary by another laboratory, they do not interact. The only reported COX-1 splice variant to exhibit cyclooxygenase activity has been isolated from …

Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck

Theses and Dissertations

Background: Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most important. A mouse model of such a type XI collagen defect is the chondrodysplasia (cho) mutant. Mice homozygous for this null mutation in the Col11a1 gene do not express the α1 chain of type XI collagen. This results in a functional knockout of type XI collagen, leading to insufficient skeletal development and perinatal lethality. …

Computational Modeling Of The Dielectric Barrier Discharge (Dbd) Device For Aeronautical Applications, Christopher S. Charles

Theses and Dissertations

Dielectric Barrier Discharge (DBD) type devices, when used as plasma actuators, have shown significant promise for use in many aeronautical applications. Experimentally, DBD actuator devices have been shown to induce motion in initially still air, and to cause re-attachment of air flow over a wing surface at a high angle of attack. This thesis explores the numerical simulation of the DBD device in both a lD and 2D environment. Using well established fluid equation techniques, along with the appropriate approximations for the regime under which these devices will be operating, computational results for various conditions and geometries are explored. In …

Jimmy Hit His First Home Run, Eileen N. Rafferty

Theses and Dissertations

This document begins with the end of a life and ends with the beginning of hope. It is a brief description of the artist's history, artistic and literary influences, and subsequent works produced during graduate school, specifically Dichotomy, Swan Dive, and Jimmy Hit His First Home Run. Topics discussed include Human Physiology, Transference, Buddhism, and Bubbles. This document was created in Microsoft Word 2004 for Mac, Version 11.2.

Small Molecules Binding To Serpins, Junaid Afridi

Theses and Dissertations

Serpins are a unique breed of proteins due to their enzymatic mechanism. Two systems were closely monitored during fluorescent binding studies, the ACT-CHY along with the AT:TRY interaction. Four different conformational variants of each system were studied including the native, cleaved, latent and complex forms. Three different fluorescent dyes were used to identify the conformations including ANS, TNS, and bis-ANS. SI studies and protease assays utilizing both Suc-AAPF-pNA and L-BAPNA were instrumental in determining conformations along with gel electrophoresis studies. The hydrophobic dyes bound to the different serpins with varying KD and ΔFmax due to structural variations among the conformers …

Reliability And Validity Of Virtual Build Methodology For Ergonomics Analyses, Tinghao Wu

Theses and Dissertations

This study was conducted to assess the validity and reliability of the Virtual Build methodology for ergonomics design and analysis. Thirty-six human subjects participated in this study and performed a set of six tasks. The tasks were performed twice in both real and virtual environment. The subject?s motion in performing tasks was analyzed by ergonomics assessments by using Virtual Build methodology. Criteria-related validity was evaluated by comparing the Virtual Build ergonomic assessment results with manual calculation. Test-retest reliability was evaluated by correlating ergonomics assessment results between two trials. The result shows that the Virtual Build methodology is reliable for ergonomic …

Synthesis And Characterization Methods Of Palladium-Doped Ceria-Zirconia Compounds, Melissa Kaye Graves-Brook

Theses and Dissertations

The main automotive catalytic media, precious metal-doped ceria-zirconia oxides, fundamental system character is unknown. Understanding the catalytic system properties should allow for the production of an optimal model catalyst. This goal of this study is to gain understanding of ceria-zirconia-palladium systems and to determine a reproducible method for preparing catalysts with minimal surface-carbon. This study investigates ceria-zirconia-palladium catalyst preparation via aqueous chemistry methods and a sputter deposition technique for doping ceria-zirconia oxide mixtures. Prepared catalysts are characterized by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). XPS allows the surface species, after catalyst doping and …

An Investigation Into Palladium-Catalyzed Reduction Of Perchlorate In Water, Eric G. Barney

Theses and Dissertations

In this study, application of a catalytic reactor to facilitate chemical reduction of perchlorate was investigated. Palladium-coated pellets were used as the catalyst, and formic acid was used as the reductant. Reactor performance was evaluated under a variety of operating conditions (influent pH, reductant concentration, residence time). Very little perchlorate reduction was observed under any operating condition. At best, approximately 8% perchlorate reduction was observed. This small reduction efficiency is clearly not sufficient for environmental treatment applications. Perchlorate strongly adsorbed to the catalyst at low pH (3 - 3.3). At higher pH (4 - 10), little adsorption was observed. This …

In Vitro Toxicity Of Cadmium Oxide Particles In Brl 3a Rat Liver Cells, Janice M. Shelley

Theses and Dissertations

For the last decade or so, in vitro toxicology has been studied as a method for determining human toxicity, as well as hazard identification and characterization. One purpose of in vitro studies is to determine the possibility of developing systems in which the toxicity of chemicals can be quantified, without heavy reliance on animal experimentation. Specific data pertaining to cadmium toxicity in humans through use of in vitro methods and models is sparse, at best. There is a need to complete more studies and collect more data to study particular chemicals and their effects and to complete proper evaluation and …

Method Of Infant Feeding As A Predictor Of Maternal Responsiveness, Emily Eiwen Drake

Theses and Dissertations

Infants need to develop effective, secure attachment to their primary caregivers in the first year of life. Researchers have not been able to identify all the factors that may influence the development of infant attachment. Most of the studies in this area have been done without regard to infant feeding as a potential factor. Maternal responsiveness appears to be key in the child's development of secure attachment behaviors, yet even after decades of research on infant attachment and maternal responsiveness, there is little evidence available to assist with early identification of families at risk and few interventions known to be …

Technical Feasibility Assessment Of Lunar Base Mission Scenarios, Trygve Magelssen Ii

Theses and Dissertations

No abstract provided.

Prehistoric Timberline Adaptations In The Eastern Uinta Mountains, Utah, Michelle Knoll

Theses and Dissertations

Excavations at a high altitude archaeological site (3350 m) in the eastern Uinta Mountains, Utah, uncovered at least three ephemeral brush structures. These temporary timberline dwellings are the highest structures excavated in Utah to date. The periods of occupation range from the early Fremont period to the post-contact era. It is believed that the Fremont occupations are logistical in nature, possibly representing male hunting parties. Logistical camps imply a departure from, and return to, a residential camp. Ethnographic studies show that most residential camps are located within proximity to culinary plants to facilitate collection by women. In the Uinta Mountains, …

Implementation And Validation Of A Real-Time Wireless Non-Invasive Physiological Monitoring In A High-G Environment, H. Aydin Akcivi

Theses and Dissertations

The overall purpose of this research is to develop a system capable of real-time personal positioning and physiological monitoring. The system will be composed of a shirt having non-invasive physiological sensors, Global Positioning System (GPS) receiver, wireless data transceiver, and real-time PC-based control station. The specific purpose of this research phase was to determine the performance capabilities of a modified LifeShirt™ alone (without GPS) in a high gravitational force environment with the data being sent wirelessly in real-time. The LifeShirt™ was modified with a real time wireless transmission system.

The General Public's Knowledge And Perceptions Of The Certified Athletic Trainer's Professional Role And Educational Background, Casey Christy

Theses and Dissertations

This study's purpose was to determine the general public's knowledge, attitudes and behaviors relative to the certified athletic trainer's professional role and educational background.

Two-hundred and seventy-nine people completed an 11-question intercept survey. Results indicate the athletic training professional faces many perception barriers with the general public.

Only 12 percent identified an "athletic trainer" as one of the on-field healthcare providers for injured professional football players. More than half failed to correctly describe what an athletic trainer does. Many responses indicate the public confuses certified athletic trainers with "personal trainers" and other strength and conditioning professionals. Eighty-four percent of the …

A Pharmacokinetic Study Of The Effects Of Stress On Chemical Exposure, Sierra H. Suhajda

Theses and Dissertations

Following the Gulf War there were many concerns raised about human exposure to the combination of chemicals and stress. Stress causes several changes in basic functions of a human body, from breathing and blood flow changes to hormonal and enzyme changes, and increased permeability of the blood-brain barrier. Each of these changes can strongly influence chemical uptake, distribution, and accumulation in the body. The purpose of this thesis was to model and predict the changes that will occur when stress is combined with chemical exposure. Physiologically-based pharmacokinetic (PBPK) modeling is one tool that can be used to visualize, predict, and …

Improving Uav Handling Qualities Using Time Delay Compensation, Andrew J. Thurling

Theses and Dissertations

This research investigated control loop time delay and its effect on UAV handling qualities. Compensation techniques to improve handling qualities in the presence of varying amounts of time delay were developed and analyzed. One technique was selected and successfully flight-tested on a UAV. Flight-testing occurred at a constant flight condition with varying levels of additional time delay introduced into the control loop. Research pilots performed a pitch tracking task and gave Cooper-Harper ratings and comments. Tracking errors were used as a quantitative measure of Pilot/Display/UAV system performance. Predictive pitch compensation was found to significantly reduce pilot workload and improve Cooper-Harper …

Scan-It: A Computer Vision Model Motivated By Human Physiology And Behavior, John G. Keller

Theses and Dissertations

This dissertation details the development of a new computational vision model motivated by physiological and behavioral aspects of the human visual system. Using this model, intensity features within an artificial visual field of view are extracted and transformed into a simulated cortical representation, and a saccadic guidance system scans this field of view over an object within an image to memorize that object. The object representation is thus stored as a sequence of feature matrices describing sub-regions of the object. A new image can then be searched for the object (possibly scaled and rotated), where evidence of its presence is …

Coronary Heart Disease Risk Factors In Collegiate Football Players, Debra A. Evenson

Theses and Dissertations

No abstract provided.

Feature Saliency In Artificial Neural Networks With Application To Modeling Workload, Kelly A. Greene

Theses and Dissertations

This dissertation research extends the current knowledge of feature saliency in artificial neural networks (ANN). Feature saliency measures allow for the user to rank order the features based upon the saliency, or relative importance, of the features. Selecting a parsimonious set of salient input features is crucial to the success of any ANN model. In this research, several methodologies were developed using the Signal to Noise Ratio (SNR) Feature Screening Method and its associated SNR Saliency Measure for selecting a parsimonious set of salient features to classify pilot workload in addition to air traffic controller workload. Candidate features were derived …

The Accuracy Of Endotracheal Tube Cuff Pressure Estimates Made By Certified Registered Nurse Anesthetists, BegoñA Kendall

Theses and Dissertations

No abstract provided.

Alcohol Consumption Patterns Among American Indian Females Who Reside On The Fort Totten Indian Reservation, Sheree L. Carlson

Theses and Dissertations

No abstract provided.