Digital Commons Network^™

A Comparison Of Attention Bias Paradigms Related To Insomnia, Nathaniel Maloney

Theses and Dissertations

Attentional bias to sleep related stimuli by poor sleepers has been reported as a factor in initiating and maintaining sleep disturbance. In this study, three paradigms (Flicker, Posner, and Stroop) were used in comparison to further investigate the role of attentional biases in Primary Insomnia. Participants (n=117) were split into good sleepers and poor sleepers using a median split with the Pittsburgh Insomnia Rating Scale (PIRS). Using the Center for Epidemiologic Studies Depression Scale (CES-D) as the covariate, two-way mixed modality ANCOVAs were conducted for each paradigm revealing no significant differences in word type, quality of sleep, or the interaction …

The Effects Of Neurotensin On The Rat Distal Colon, Rick Chainani

Theses and Dissertations

The enteric nervous system controls the gut through the release of specific neurotransmitter and neuromodulators at specific sites such as mucosal secretory cell or smooth muscle cell. In the present study, we have examined the response to one of these neurohumoral agents, Neurotensin, in the rat distal colon. Neurotensin is a paracrine and endocrine modulator of the digestive tract. Even though these effects have been seen in colonic preparations, there are very few functional studies of the effects of Neurotensin in the rat colon, especially the distal colon. In the current study we propose the following hypothesis that Neurotensin will …

The Role Of Outer Membrane Protein A In Anaplasma Marginale Cellular Invasion And Its Potential As A Cross-Protective Antigen, Sarvani Emani

Theses and Dissertations

Anaplasma phagocytophilum and A. marginale are the etiologic agents of human granulocytic anaplasmosis and bovine anaplasmosis, respectively. Both diseases can be severe, even fatal, and protective vaccines for each are lacking. We recently identified A. phagocytophilum outer membrane protein A (ApOmpA) as being critical for cellular invasion and is expressed during infection of mammalian but not tick cells. Disrupting ApOmpA-host cell interactions significantly inhibits A. phagocytophilum entry into host cells. ApOmpA and its A. marginale ortholog, AM854 (A. marginale OmpA; AmOmpA) exhibit 44% amino acid identity. The ApOmpA invasin domain is highly conserved between both proteins. In this study, we …

An Analysis Of The Marketing Tactics Used To Influence Millennial Generation Students In Their Decision To Attend A Two-Year Or A Four-Year College, Andrea Stanton

Theses and Dissertations

This study will attempt to evaluate the effectiveness of college recruitment marketing tactics used to influence Millennial Generation students, ages 17 to 22 years old, in their decision to attend either a two-year or a four-year college. Changing demographics and projections indicate a decline in the number of high school graduates within the next three to five years. This shift translates into fewer college applications and more competition among institutions of higher learning making it easier for most students to get accepted into college. The researcher will use focus groups and a survey to examine the perception of community colleges …

The Role Of Nitric Oxide Dysregulation In Tumor Maintenance, Christopher Rabender

Theses and Dissertations

The inflammatory nature of the tumor microenvironment provides a cytokine and chemokine rich proliferative environment. Much of the responsibility of this environment is due to the production of Reactive Oxygen Species (ROS). These studies examined the proliferative rich tumor environment from a new perspective of Nitric Oxide Synthase (NOS) dysregulation. NOS’s have the ability to become uncoupled and generate superoxide in lieu of nitric oxide (NO). A requirement of NOS for the production of NO is the cofactor tetrahydrobiopterin (BH4) and when it is missing NOS becomes uncoupled and turns into a peroxynitrite synthase. Here I demonstrate that NOS is …

The Effects Of Planting Techniques On Maize Grain Yield And Silage Production, Tyler D. Kaufman

Theses and Dissertations

This thesis is a comprehensive analysis of the effects of seed orientation, row direction and planting population on grain yield, kernel composition, and silage yield and composition. Producers are striving to increase profit by efficiently using their available land area to maximize crop production. However, little research is available testing the effect of seed orientation on grain or silage production. Quantifying the impacts of seed orientation and row direction on grain and silage is essential for maximum crop production. Dissemination of the results should benefit grain and silage producer.

Two experiments were conducted to evaluate the effects of manipulating seed …

The Effect Of Choral Music Education On Literacy Achievement, Keith Alan Hearnsberger

Theses and Dissertations

American education was going through dramatic change in the beginning of the 21st century. The way children were educated was constantly evolving. Public schools, private schools, parochial schools, charter schools, and many other types of educational institutions were affected by various federal and state regulations since the 1960's. The students, teachers, and educational leaders in these institutions were consistently affected by regulations, increased academic demands, and an ever-changing educational landscape. The educational administrator and music educators were required to be innovative when seeking change in policy in regards to educational achievement while maintaining a commitment to choral music education. The …

Human-Swarm Interaction: Effects On Operator Workload, Scale, And Swarm Topology, Brian O. Pendleton

Theses and Dissertations

Robots, including UAVs, have found increasing use in helping humans with dangerous and difficult tasks. The number of robots in use is increasing and is likely to continue increasing in the future. As the number of robots increases, human operators will need to coordinate and control the actions of large teams of robots. While multi-robot supervisory control has been widely studied, it requires that an operator divide his or her attention between robots. Consequently, the use of multi-robot supervisory control is limited by the number of robots that a human or team of humans can reasonably control. Swarm robotics -- …

Image Annotation And Topic Extraction Using Super-Word Latent Dirichlet, George E. Noel Iii

Theses and Dissertations

This research presents a multi-domain solution that uses text and images to iteratively improve automated information extraction. Stage I uses local text surrounding an embedded image to provide clues that help rank-order possible image annotations. These annotations are forwarded to Stage II, where the image annotations from Stage I are used as highly-relevant super-words to improve extraction of topics. The model probabilities from the super-words in Stage II are forwarded to Stage III where they are used to refine the automated image annotation developed in Stage I. All stages demonstrate improvement over existing equivalent algorithms in the literature.

Architecting Integrated System Health Management For Airworthiness, Kerwin C. Teong

Theses and Dissertations

Integrated System Health Management (ISHM) for Unmanned Aerial Systems (UAS) has been a new area of research - seeking to provide situational awareness to mission and maintenance operations, and for improved decision-making with increased self-autonomy. This research effort developed an analytic architecture and an associated discrete-event simulation using Arena® to investigate the potential benefits of ISHM implementation onboard an UAS. The objective of this research is two-fold: firstly, to achieve continued airworthiness by investigating the potential extension of UAS expected lifetime through ISHM implementation, and secondly, to reduce life cycle costs by implementing a Condition-Based Maintenance (CBM) policy with better …

Synthetic Polymeric Carbohydrates Recognized Toxoplasma Gondii Tachyzoites And Induced Early Phagocytosis By Macrophages, Souzan Hussain Eassa

Theses and Dissertations

Toxoplasmosis is a parasitic disease caused by the intracellular protozoan Toxoplasma gondii your words (T. gondii your words) that affects most types of warm-blooded vertebrate cells. Up to one third of the humans population in the world is chronically infected by this parasite. Human and animals at risk for toxoplasmosis include fetuses, newborns, and immunologically impaired patients. To date, there is no vaccine available and no drug has a successful effective against chronic form of toxoplasmosis. Recent Toxoplasma your words diagnostic methods showed high false positive results and cannot detect the parasite in the early infection stage. Early diagnosis of …

Development Of The In Vivo Multicolor Photoacoustic Flow Cytometer, Mustafa Sarimollaoglu

Theses and Dissertations

Flow cytometry is a powerful tool for analysis of cells in artificial flow. This highly accurate technology, using multicolor fluorescent probes and lasers with different wavelengths, provides fast multiparameter quantification of the biological properties of cells. Invasive extraction of cells from an organism, however, leads to changes in cell properties and low sensitivity, and prevents studying cells in their native environment. Challenges to advances in in vivo flow cytometry include fluorescent probe toxicity, strong background, and number of colors, as well as assessment of small vessels. To overcome these limitations, photoacoustic flow cytometry (PAFC) was introduced. PAFC is based on …

Functional Characterization Of Crip1a Knockout Mice, Joanna Jacob

Theses and Dissertations

CB1 cannabinoid receptors are G-protein-coupled receptors that mediate the central nervous system (CNS) effects of marijuana and endocannabinoids. Recently, cannabinoid receptor interacting protein 1a (CRIP1a) was discovered as a novel protein that binds to the CB1 receptor C-terminus and inhibits CB1 receptor activity without affecting CB1 expression. This thesis investigated the functions of CRIP1a by characterizing the first CRIP1a knockout (KO) mouse line. The absence of CRIP1a was confirmed in KO mice using quantitative PCR and immunoblotting. I hypothesized that CRIP1a KO mice would exhibit enhanced CB1 receptor-mediated G-protein activity in the CNS, as well as cannabimemetic phenotypes and enhanced …

Positron Emission Tomography For Pre-Clinical Sub-Volume Dose Escalation, Christopher Bass

Theses and Dissertations

Purpose: This dissertation focuses on establishment of pre-clinical methods facilitating the use of PET imaging for selective sub-volume dose escalation. Specifically the problems addressed are 1.) The difficulties associated with comparing multiple PET images, 2.) The need for further validation of novel PET tracers before their implementation in dose escalation schema and 3.) The lack of concrete pre-clinical data supporting the use of PET images for guidance of selective sub-volume dose escalations. Methods and materials: In order to compare multiple PET images the confounding effects of mispositioning and anatomical change between imaging sessions needed to be alleviated. To mitigate the …

Cytotoxicity Study Of Iron Oxide Nanoparticles, Single-Wall Carbon Nanotubes And Their Complexes Applied To Mcf7 Breast Cancer Cells, Karine Mege

Theses and Dissertations

Reactive Oxygen Species (ROS) are radicals of great concern to biologists. Their role in several diseases--such as neurodegenerative disease, diabetes, premature aging and cancer--has been intensively investigated during the last decade. Since a major focus in cancer research is to better understand how it is induced and therefore how it can be cured, the study of the cytotoxic effects of ROS production within cancer cells is vital. Nanotechnology is an emerging field of science that promises great improvements in a number of disciplines. Nano medicine is one of its daughter fields. Various nanomaterials are used for diagnosis and disease detection, …

Synthesis And Characterization Of Polypyrrole-Metal Complexes As Electrocatalysts, Nandan K. Peddi

Theses and Dissertations

Modern fuel cells convert chemical energy to electrical energy due to the reaction between hydrogen and oxygen to produce water using a platinum electrocatalyst. The scarcity and high cost of platinum have led to the development of various less expensive and efficient electrocatalysts. Polypyrrole-cobalt (CoPPy) complex has been developed as a nonplatinum electrocatalyst for oxygen reduction reaction (ORR) and potential application as a cathode in fuel cells. The complex was synthesized by refluxing pyrrole monomer and cobalt acetate tetrahydrate in dimethylformamide (DMF) for two hours followed by vacuum distillation to collect the solid electrocatalyst. Cyclic voltammetry, rotating disk electrode voltammetry …

Breastfeeding: An Analysis And Community-Based Curriculum For The Contemporary Central Arkansas Audience, Jennifer Susan Atkins-Gordeeva

Theses and Dissertations

Contemporary society includes many features that impede women's decision-making about infant feeding. The Central Arkansas community is particularly affected by those features. Because informed decision-making involves an acknowledgment of the risks of formula use, breastfeeding advocates must talk about risk. This curriculum offers one way to discuss risks of formula and challenges and supports within the community, to create a supportive environment for making informed decisions, and to commit to a lactation plan, inside a supportive, community-based setting.

Alexander Volkov And The Dancing Bacha: Uzbek Motifs In Motion, Rachel S. Golden

Theses and Dissertations

Alexander Volkov (1886-1957), an Uzbek artist identified by his expressive style, vibrant palette, and devotion to folk subjects, was a leading figure in the movement known as the Uzbek avant-garde. Volkov's influence is still at work among artists in Uzbekistan, though his legacy remains largely unexplored by Western scholars. The Arkansas Arts Center owns one drawing by Volkov depicting the tradition of dancing boys or bachas, a subject the artist also painted among his reflections on Uzbek culture. Work from the same period by fellow artist Alexander Nikolaev (Usto Mumin, 1897-1957), who was better known for his attachment to bachas, …

How I Saved My Horses' Hooves: An "Old School" Cowboy Goes Natural, James Karl Dawson

Theses and Dissertations

How I Saved My Horses' Hooves: An "Old School" Cowboy Goes Natural is an essay written to persuade horse owners to choose natural hoof care over traditional shoeing. It is an argument against nailing iron shoes on the hooves of horses. My intent is to persuade horse owners to provide their horses with every means possible to allow them to shape their hooves naturally. Doing so will strengthen their hooves to the point where they will no longer need shoes. Domestic horses cannot live normal lives, therefore, they cannot grow and naturally shape their hooves unless we offer what nature …

Validation Study Of A Novel Detection Kit For Rapid Detection And Quantification Of Listeria Spp. In Food Samples, Mengying Jiang

Theses and Dissertations

A single tube detection kit was designed as a rapid, easy-to use and reliable test to detect Listeria spp.. Various food samples (vegetables and raw catfish fillets) were used in order to validate the performance of the detection kit. L. grayi was detected in one ready-to-eat (RTE) vegetables with the detection kit while no Listeria spp. was detected using the modified FDA-BAM method. In addition, both the detection kit and modified FDA-BAM method indicated that twelve catfish fillets were Listeria positive. The detection kit had 100% sensitivity and specificity in less detection time (24 h) than the modified FDA-BAM method …

Anticoagulant Activity Of Inhaled Heparin In The Dog, Jill S. Manion

Theses and Dissertations

Respiratory disease represents an important component of small animal emergency medicine. The morbidity and mortality of respiratory disease and inflammation, although poorly defined, is considered to be significant. Much of the therapy used in the stabilization and management of respiratory disease in veterinary patients has been taken from human medicine, including inhalation therapy. Heparin has been shown to have substantial anticoagulant, anti-inflammatory, and antiibrotic effects within the lungs when administered via inhalation in human patients. To date, no studies have evaluated the use of nebulized heparin in dogs. This study is the first to attempt to generate pharmacokinetic data regarding …

Antioxidative And Antimicrobial Activity Of Casein Hydrolyzate In Cheddar Whey-Based Edible Coatings, Yin Zhang

Theses and Dissertations

Hydrolysis of casein using chymotrypsin results in the formation of polypeptides (CH) with a hydrophobic aromatic amino acid on one end of the chain because the enzyme selectively cleaves the adjacent peptide-bond. Due to resonance of the aromatic micro-domain, thiols become redox-sensitive and actively participate in electron transfer. These types of amphipathic peptides also tend to be membrane-lytic. The two prong approach of this investigation was to, (1) assess antibacterial effect of the CH in beef steak, and (2), to determine its antioxidative efficacy as a constituent of Cheddar whey based edible coating mix. The edible coating prevented coliform growth …

Transcriptome Response Associated With Protective Immunity In T And B Cell Deficient Zebrafish, Aparna Krishnavajhala

Theses and Dissertations

RAG1-/- mutant zebrafish lack T and B lymphocytes. However, when re-exposed to homologous bacteria, these fish mount a response that provides specific protection. To further define this response, we utilized microarray analyses to determine the mechanisms underlying innate immune system memory in zebrafish. We also analyzed interferon (IFN) gamma by qRT-PCR. It is produced by activated NK cells and could indicate if this cell mediates the protective response seen in lymphocyte deficient zebrafish. Pathological studies and in situ hybridizations were performed to observe tissue changes and location of the cells that produced IFN gamma. Following bacterial re-exposure, zebrafish transcripts in …

Cattle Grazing Preferences, Animal Performance, And Harvest Management Effects Among Diploid And Tetraploid Cultivars Of Annual Ryegrass, Juan Kevin Quamina Solomon

Theses and Dissertations

Incremental gains in productivity from new forages are likely to be very small in developed countries like the USA where forage research is highly advanced, thus animals must be used in determining their value. Three experiments were conducted. Experiment I evaluated grazing preference of cattle and its relationship with morphological and chemical characteristics. Treatments were two diploid cultivars ‘Marshall’ and ‘Gulf’ and two tetraploid cultivars ‘Maximus’ and ‘Nelson’ arranged in a 4 × 4 Latin square design experiment. Animal preference was based on herbage disappearance, the Chesson-Manly index, and animal grazing time. Both herbage disappearance (1400 vs. 890 kg ha-1) …

Changes In Host Use By Unionid Mussels Following River Channelization And Impoundment, Lee Gray Turnage

Theses and Dissertations

More than half the North American freshwater mussel species in the family Unionidae (unionids) are imperiled or extinct. Alteration of rivers is considered a major contributor to unionid population declines. Losses could occur through disruption of the reproductive cycle. Unionid reproduction requires attachment of larva (glochidia) to host fishes; therefore, changes in the host fish community could alter the reproductive potential in unionid communities. There have been few attempts to compare reproductive success before and after alteration. I examined the pattern of glochidia use on two common host fishes, Lepomis megalotis and Cyprinella venusta, before and after alteration of the …

Comparison Of Student Achievement Among Two Science Laboratory Types: Traditional And Virtual, Mary Celeste Reese

Theses and Dissertations

Technology has changed almost every aspect of our daily lives. It is not surprising then that technology has made its way into the classroom. More and more educators are utilizing technological resources in creative ways with the intent to enhance learning, including using virtual laboratories in the sciences in place of the “traditional” science laboratories. This has generated much discussion as to the influence on student achievement when online learning replaces the face-toace contact between instructor and student. The purpose of this study was to discern differences in achievement of two laboratory instruction types: virtual laboratory and a traditional laboratory. …

Serenbe, A Case Study In Agricultural Urbanism, Charles Alexander Gotherman

Theses and Dissertations

Despite the overwhelming role food plays in our everyday lives, the both new and old phenomenon of incorporating food systems into master planned communities has largely been overlooked. The in-progress community of Serenbe located just outside of Atlanta, Georgia is breaking this trend. The mixed use 1,000 acre community has already included many food and agriculture elements such as an organic farm, farmers’ market, grocery store, farm-to-table restaurants and edible landscaping - making it an exemplar case of an emerging planning strategy called Agricultural Urbanism. While many are heralding Serenbe for its strides as a community that incorporates agriculture into …

Assessment Of Canine Immunity Using Computational And Flow Cytometric Approaches, Kriston Weaver

Theses and Dissertations

The Affymetrix GeneChip® Canine Genome 2.0 microarray is re-annotated using AgBase tools, up-to-date ID mapping and GO annotations associated with publicly available gene products updated on this array. This re-annotation makes the array more useful for researchers using the canine microarray for biological discovery. We use flow cytometry to determine if liposomal clodronate (LC) is an acceptable alternative to surgical splenectomy to facilitate detection of subclinical infection with Babesia canis in potential blood donor greyhounds. Our study shows that LC is not a reliable means of exposing babesiosis in greyhounds with a recent history of infection. We evaluate the effect …

Characterization Of Novel Virulence Factors Of Listeria Monocytogenes And Their Roles In Pathogenesis, Ting Zhang

Theses and Dissertations

The pathogenicity of food-borne intracellular bacterium Listeria monocytogenes is greatly associated with its abilities to invade non-phagocytic cells, counteract the host innate immune system, resist bactericidal antibiotic-mediated killing, and breaking the physical barriers. In the last 30 years of research on L. monocytogenes, several virulence factors, such as Listeriolysin O (LLO), InlA, InlB, ActA, PI-PLC, and PC-PLC have already been characterized as important players that help this bacterium to achieve the key stage of infection. There are approximately 3,000 open reading frames in Listeria’s genome; however, only few virulence factors are functionally characterized. Thus, it is important to identify new …

Diversity And Characteristics Of Heat-Stress Adaptation In Listeria Monocytogenes Strains, Priyanka Mahesh Jangam

Theses and Dissertations

A set of 37 strains including 13 serotypes of Listeria monocytogenes (Lm) were analyzed for heat tolerance at 60°C for 10 min and further categorized into three groups; low (strains with <2 log survival), medium (2-4 log survival), and high (4-6 log survival) heat tolerant. When Lm strains representing each group were subjected to sub-lethal heatstress at 48°C prior to 60°C, the survivals of all strains were increased by at least 5 log CFU/ml when compared to controls. Sub-lethal heat-stress at 48°C for 30-60 min increased the heat-stress resistance of Lm strains by doubling D60°C values from 1.9-4.3 to 5.0-10.4 min. When Lm cells were cooled after sublethal heat-stress at 48°C prior to 60°C treatment, such acquired heat-stress adaptation was unstable at 22°C but was found to be highly stable for up to 24 h at 4°C. These results will have potential implications in food safety risk analysis for Lm.