Digital Commons Network^™

Understanding Language Use Patterns Of People With Chronic Pain: A Novel Assessment Approach, Jodie Maccarrone

Theses and Dissertations

It is estimated that 35% of Americans or 116 million people report experiencing chronic pain daily (Nahin, 2015). Due to the unique and individualized nature of chronic pain, successful treatment of this condition is difficult to achieve. The heterogeneity and complexity in presentation may help explain why, as all methods of treatment have fallen short of providing consistent and reliable outcomes (Finnerup et al., 2015; Lumley & Schubiner, 2019), resulting in unsuccessful treatment approaches that can lead to compounding negative consequences such as hyperalgesia (Garland et al., 2013) and even death (Scholl, Seth, Kariisa, Wilson, & Baldwin, 2019). As such, …

Objectified: Forced Marriages And Bitter Reality Of Violence Against Women In India, Anurag Wallace

Theses and Dissertations

Domestic violence against women is an ongoing problem in India. With cases rising ever higher, the time has come to talk openly about the uncomfortable truths behind arranged marriages, which foster injustice and often lead to violence against women. The wedding dress is a symbol of purity in traditional Indian weddings, but in the case of marriages that turn abusive, it can become a symbol of oppression and patriarchy. During the research phase of this investigation, women once stuck in abusive marriages—treated as objects—talked about the objects that made them feel trapped and explained how these everyday objects became silent …

Viral Overexpression Of Rprd2 In Mouse Nucleus Accumbens Induces Anxiety-Like Behavior And Regulates Genes Associated With Microtubule Transport, Claire Nuhad Atiyeh

Theses and Dissertations

Anxiety disorders are the most prevalent mental illness in the United States with many individuals underdiagnosed and undertreated (Anxiety & Depression Association of America, 2021; Bandelow et al., 2017). While stress is a known risk factor for developing anxiety disorders, there is emerging evidence that biological variability in the expression of key proteins in the brain may contribute to regulating an organism’s stress-related behaviors. Earlier work from our laboratory identified a protein, called Regulation of Nuclear Pre-mRNA Domain Containing 2 (RPRD2), that was differentially expressed in the brain of mice stratified in terms of their phenotypic response to chronic stress …

The Role Of Irf-1 In Spontaneous Mouse Glioma, Aakash B. Vaidya

Theses and Dissertations

Glioblastoma Multiforme has been shown to be one of the deadliest primary brain cancers. One of the reasons why GBM is so deadly, is a unique immunosuppressive tumor microenvironment that promotes GBM growth and progression. Both astrocyte and microglia have been implicated in immunosuppression. In this study, we explored the role of Interferon Regulatory Factor 1 (IRF-1) in astrocytes and glioma cells on the growth of spontaneous glioma tumors. IRF-1 is regulated by the JAK/STAT pathway and induces expression of Programmed death ligand 1 (PD-L1). PD-L1 downregulates immune responses to glioma. We found that IRF-1 had no effect on spontaneous …

Outcome Of Endodontic Microsurgery: Success And Survival Rates At Virginia Commonwealth University, Abdullah Alawadhi

Theses and Dissertations

Introduction: Endodontic microsurgery treatment is an option for teeth with apical periodontitis and may be indicated for teeth with previous endodontic treatment that have not healed, teeth with canal obstructions which would impede a non-surgical approach, and where a biopsy is needed. The purpose of this study is to evaluate healing after endodontic microsurgery in 2-dimensional PA radiographs and 3 dimensional CBCT imaging. Methods: Patient records were pooled from graduate resident cases completed at Virginia Commonwealth University in the Graduate Endodontic Clinic between the years 2010 to 2020. Cases were identified using the CDT codes and a key word search …

The Effect Of Er:Yag, Nd:Yag And Co2 Laser Combined With Hydrogen Peroxide, Sodium Hypochlorite, Chlorohexidine Or Fluoride On Reducing Oral Bacterial Count Implicated In Root Caries, Nitya Reddy

Theses and Dissertations

Purpose: Lasers have been used for treatment of dentinal hypersensitivity and for various bacterial reduction indications in periodontology. Their effectiveness in killing oral bacteria is not well known. The compounding effect of the combination of a laser treatment and adjunct antimicrobial agent use on bacterial viability is still evolving. The purpose of this in vitro study was to evaluate the effect of three lasers commonly used in dentistry in conjunction with chlorhexidine (CHX), hydrogen peroxide (H₂O₂), sodium hypochlorite (NaOCl), or sodium fluoride (NaF) on viability of oral bacteria associated with root caries.

Methods: Three …

Characteristics Of Posterior Permanent Teeth With A Periradicular Diagnosis Of Symptomatic Apical Periodontitis: A Retrospective Study, Christina A. Martin

Theses and Dissertations

The diagnosis of apical periodontitis is based on radiographic manifestations and the results of the various clinical tests that can be performed as part of a routine dental examination. Apical periodontitis producing clinical symptoms involving a painful response to biting and/or percussion or palpation is given the diagnosis of symptomatic apical periodontitis (SAP). This study aimed to determine associations of SAP with gender, age, radiographic findings, diagnostic testing, pulpal diagnosis, and location of the tooth in the arch. Data for this study were obtained from patients of record treated at Virginia Commonwealth University School of Dentistry in the Department of …

Progress Towards Next Generation Modulators Of Ctbp's Oncogenic Effects, Jacqueline L. West

Theses and Dissertations

Human C-terminal binding proteins (CtBP1 and CtBP2) are transcriptional coregulators of multiple genes in the human genome, including tumor suppressor genes (e.g., Bik, PTEN, BRCA1, and E-cadherin) as well as oncogenes (e.g. MDR1 and Tiam1). Both homologues of CtBP are overexpressed in many types of cancer, including breast cancer (92%), ovarian cancer (83%), colorectal cancer (64%), hepatocellular carcinoma (60%), gastric cancer, prostate cancer, and pancreatic adenocarcinoma. Further, expression levels of CtBP correlate with worse prognostic outcomes and more aggressive tumor features because it promotes proliferation, epithelial-mesenchymal transition, and cancer stem cell self-renewal activity.

Our laboratory has identified a lead inhibitor …

Again Black Again, Muthi Reed

Theses and Dissertations

ABSTRACT

AGAIN BLACK AGAIN

by muthi reed, M.F.A

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Fine Arts at Virginia Commonwealth University.

Program Director: Stephen Vitiello

Committee: Semi Ryu, Orla McHardy, MK Abadoo, Cara Benedetto

AGAIN BLACK AGAIN. ABA-BBABB is documentation of a reparations practice and articulations for a reparations manifesto devised by the artist. Reparations begins with the self. Singing truth to the self. Talking with the self. The thesis organizes around the problems of surveillance and myth making about Black people and the subsequent source of self-regard, conjure and social dreaming …

Viral Injection Of Rna Polymerase Ii-Interacting Protein Rprd2 In The Nucleus Accumbens Induces Anxiety-Like Behavior In Mice, Hannah E. Woolard

Theses and Dissertations

Anxiety and its related disorders have become increasingly prevalent as more awareness and acceptance of mental illnesses have come to fruition, especially in the light of the ongoing COVID-19 pandemic. Anxiety can affect anyone regardless of their age, sex, or social status and is currently the most commonly diagnosed mental illness worldwide (Bandelow & Michaelis, 2015). While there are several effective treatments available, the underlying brain mechanisms that cause anxiety are still largely unknown and further research continues to piece together the complex pathophysiology behind this disease. The use of laboratory animal models, such as mice, to induce and observe …

Targeting Bcl-2 Family Proteins In Therapy Induced Senescent Cancer Cell Models, Wade Cook

Theses and Dissertations

Non-small Cell Lung Cancer (NSCLC) originates from numerous different cell types in the lungs and is among the deadliest of cancers. Head and Neck Squamous Cell Carcinomas (HNSCC) are derived from the mucosal membranes of the oral cavity, pharynx, and larynx. Both NSCLC and HNSCC are predominately caused by tobacco smoke inhalation and as such mutations in the tumor suppressor gene TP53 are common. Since similarities exist in the root cause of NSCLC and HNSCC, they may also share similarities in treatment methods. Cisplatin is a platinum-based DNA damaging agent that has been used as a cancer chemotherapy for decades. …

Evaluating Population Genetic Structure And Potential Genomic Signals Of Natural Selection In A Migratory Songbird (Protonotaria Citrea), Tyler A. Hohenstein

Theses and Dissertations

In this study I attempted to further resolve the population genetic structure in the Prothonotary Warbler (Protonotaria citrea), and conducted an outlier SNP analysis and exploratory gene ontology analysis to investigate potential ongoing natural selection in the species. This analysis of population structure confirms previous work by DeSaix et al. (2019), where weak population structure was observed between eastern sites along the Atlantic Coastal Plain, and western sites in the Mississippi Alluvial Valley, possibly due to a genetic discontinuity across the Appalachian Mountains. I conducted two forms of outlier SNP analyses, a principal component analysis (PCA)-based approach to identify SNPs …

The Discovery And Investigation Of Jig (Cg14850): A Previously Uncharacterized Novel Drosophila Melanogaster Protein, Sayem Hoque Bhuiyan

Theses and Dissertations

Despite Drosophila being one of the most studied model organisms in the world of research, one-third of 14,000 protein-encoding genes that the Drosophila genome possesses code for proteins that are yet uncharacterized. While investigating some of these uncharacterized Drosophila proteins, we identified a scarce protein type–a protein localized in both mitochondria and nucleus. Proteins, dual-localized in mitochondria and nucleus, establish communication between these organelles. This type of dual-localized protein is among the rarest of proteins, which limits us from fully understanding the mito-nuclear communication mechanism. Here, we tell the story of Jig, which we discovered to be one of those …

The Architecture And Dynamics Of Gene Regulatory Networks Directing Cell-Fate Choice During Murine Hematopoiesis, Joanna Handzlik

Theses and Dissertations

Mammals produce hundreds of billions of new blood cells every day througha process known as hematopoiesis. Hematopoiesis starts with stem cells that develop into all the different types of cells found in blood by changing their genome-wide gene expression. The remodeling of genome-wide gene expression can be primarily attributed to a special class of proteins called transcription factors (TFs) that can activate or repress other genes, including genes encoding TFs. TFs and their targets therefore form recurrent networks called gene regulatory networks (GRNs). GRNs are crucial during physiological developmental processes, such as hematopoiesis, while abnormalities in the regulatory interactions of …

Mosquito Fauna And Prevalence Of Avian Malaria In The Turtle Mountains, North Dakota, Kelsey J. Morin

Theses and Dissertations

Avian malaria is a disease caused by intracellular parasites transmitted by mosquitoes and is detrimental to individual birds and their populations. Although it is sub-lethal in most instances, avian malaria can result in anemia, lower fertility, and reduced migration ability. The Turtle Mountains is a unique ecoregion in North Dakota and provides habitat for various bird and mosquito species that differ from other parts of the state. Previous research has defined the mosquito fauna and occurrence of avian malaria in the Red River Valley region of eastern North Dakota and Northwest Minnesota; however, no research has been conducted in the …

Behavioral Responses Of Breeding Ducks To Unmanned Aerial Vehicle Surveys And Best Practices For Breeding Waterfowl Surveys Using Unmanned Aerial Vehicles, Mason Ryckman

Theses and Dissertations

Unmanned aerial vehicles (UAVs) have become a popular wildlife survey tool. As such, biologists are exploring the use of UAVs for surveying waterfowl. The most cited benefit of using UAVs over traditional methods is the idea of reduced disturbance, but this has had limited formal evaluation across species. We conducted UAV surveys with associated behavioral observations of ducks on wetlands and on nests during the 2019 – 2020 breeding seasons. We found species-specific behaviors among blue-winged teal (Spatula discors), northern shoveler (Spatula clypeata), and gadwall (Mareca strepera) including ducks noticing the aircraft, but reactions were generally less than traditional ground …

Phylogenetic Relationships Of Noctuoid Moths With An Examination Of Complex Characteristics, Melissa Sisson

Theses and Dissertations

The tribe Noctuini comprise over 520 species; many are economically important species that impact human agriculture. Despite their diversity and relevance, relationships of the Noctuini have been difficult to resolve. There are extensive morphological revisions of these taxa (e.g., Agrotis, Euxoa); however, there are no studies that focus exclusively on the phylogenetic relationships of the Noctuini. Currently, the Noctuini are separated into two subtribes, the Agrotina and Noctuina, which each are further subdivided into two subgenera. In this study, we tested previous classification schemes using three loci. We sampled three outgroup taxa and 54 noctuine species that represent the majority …

Development Of The Share Your Story Program: Understanding How Self-Stigma And Mental Health Storytelling Influence Mental Health Experiences On College Campuses, Kyle Cromer Elliott

Theses and Dissertations

Mental health is a growing concern faced by college administrators. Self-stigma is a major barrier preventing mental health utilization and recovery on college campuses. The purpose of this research study is to understand how self-stigma influences the mental health experiences of college students.

The research study builds upon narrative inquiry—Honest, Open, Proud (HOP)—and the author’s lived experiences with mental health conditions to answer the research question: “How does self-stigma influence students’ mental health experiences on college campuses?”

Findings indicate that self-stigma and self-acceptance impacted if, how, and with whom research study participants shared their mental health conditions. Findings also show …

A Survey Of Research Administrators To Identify Areas Of Administrative Burden In Federal Research Grant Management, Jessica Lynn Schiller

Theses and Dissertations

Many processes in higher education are complicated by administrative burden and proceduralinefficiencies. The federal government and research universities partner to advance science and technology, but the federal research grant process is criticized for its lack of efficient and streamlined procedures. Staff and faculty alike lament losing valuable time to bureaucratic regulations, cumbersome processes, and burgeoning reporting standards. As the cost of higher education is increasingly scrutinized, current procedures must be analyzed to identify areas of excessive administrative burden and redesigned to maximize efficiency. To address this issue, a Qualtrics survey was deployed to research administrators to identify areas of administrative …

Small Mammal Community Ecology In A Northern Grassland, Lynda Lafond

Theses and Dissertations

I studied grassland small mammal community ecology at UND’s Oakville Prairie, in Grand Forks County, North Dakota summers 2018-2021. Specifically, I was interested in small mammal richness and abundance, investigating their habitat associations, investigating their phenotypic variation, and understanding their diets. I trapped small mammals for five consecutive nights at the end of June to early July 2018 – 2021. I also measured plant community composition and structure metrics. Over the four years, I captured 985 individuals from 11 species. Species richness and abundance varied spatially and temporally across the site. I most frequently captured meadow voles (Microtus pennsylvanicus), mice …

Role Of Aurora Kinase A, Rab4a, And Endolysosomes In The Development Of Alzheimer’S Disease-Like Pathology, Zahra Afghah

Theses and Dissertations

Endosomes and lysosomes (endolysosomes) are organelles that play key roles in cell survival and cell death. These acidic intracellular organelles are the principal sites for intracellular hydrolytic activity required for the maintenance of cellular homeostasis. Endolysosomes are involved in the degradation of plasma membrane components, extracellular macromolecules, as well as intracellular macromolecules and cellular fragments. Understanding the physiological significance and pathological relevance of endolysosomes is now complicated by relatively recent findings of physical and functional interactions between endolysosomes and other intracellular organelles including endoplasmic reticulum, mitochondria, plasma membranes, and peroxisomes. Indeed, evidence clearly indicates that endolysosome dysfunction and inter-organellar signaling …

Translocation Of Yops By Yersinia Pestis Into Canine Cell Lines, Betsy Louise Young

Theses and Dissertations

Yersinia pestis, the causative agent of plague, secretes a set of virulence proteins called Yops when in contact with eukaryotic cells. A subset of these Yops is translocated directly into the cytosol of the host eukaryotic cells by the type III secretion system. Using a protein tag-based reporter system, this study is focused on the translocation of these Yops into the cytosol of canine derived cell lines: MDCK and DH82. The MDCK cell line is a canine derived epithelial cell line, and the DH82 cell line is a canine derived monocyte cell line. The reporter system uses a phosphorylatable peptide …

Evaluating The Effects Of Mechlorethamine On The Dermal-Epidermal Junction, Trishaal Janardhanam Raghavendra Rao

Theses and Dissertations

Sulfur Mustard (SM) is a chemical warfare agent first utilized on the battlefield more than 100 years ago. To the present time, no antidote to combat the dermatotoxicity of SM exists. Mechlorethamine (HN2) is a derivative of SM which is used in anticancer therapy. Dermal exposure of HN2 is associated with dermal-epidermal junction (DEJ) disruption (vesication) which limits its clinical usefulness. The primary purpose of the present study was to investigate the mechanisms of vesication caused by HN2 using an in vivo mouse ear vesicant model (MEVM). The ears of male or female C57BL/ 6J mice were exposed to a …

The Experience Of Saudi Students Writing Academic English In Sex-Separated Classrooms, Rana Alsaadi

Theses and Dissertations

This is a qualitative research that investigates the experiences of undergraduate Saudi female and male students majoring in English at Taif University, Saudi Arabia. These students were part of different sections of a course that teaches academic English writing and research in an English as a Foreign Language context. These Saudi female and male students draw on their perceptions of three main points; First, how they experienced the academic English writing course in sex-separated classrooms. Second, how the current sex-separated classrooms influence their learning of English and how if this hypothetically change to mixed-sex classrooms their experiences in learning English …

Suitability Analysis Of Snow And Ice Changes For Ice Patch Archaeology: A Case Study In The Crazy Mountains, Montana, Christine Kay Lidenberg

Theses and Dissertations

As technology advances over the years there has been a growing interest in the application of using remote sensing for conveying the detection of alpine archaeological sites. As climate changes and global temperatures rise across the globe perennial snow/ ice patches are melting and disappearing in alpine areas. These alpine archaeological sites are being immensely affected and, in some cases, destroyed. The research was conducted to determine if using both remote sensing imagery and suitability models could detect the extent of perennial snow/ice patch loss and determine the ice patches most vulnerable to melting in the Crazy Mountains of Montana. …

Landscape Ecology Influence On Population Dynamics And Gene Flow: A Muskrat’S Tale, Ayla A. Morehouse

Theses and Dissertations

Understanding movement patterns across a landscape is an essential tool for wildlife managers to understand and predict population dynamics, interactions, and susceptibility to disease and environmental changes. Part of this is due to population spatial synchrony being driven by three primary factors: dispersal, the “Moran Effect”, and trophic interactions. We seek to understand if dispersal may play a larger role in population synchrony and, if so, what landscape features may hinder the movement of muskrats (Ondatra zibethicus), a small semiaquatic mammal that relies on ditches and shallow wetlands for habitat and local movement. In this study we genotyped eleven microsatellite …

Genetic Breed Association And Contraceptive Response Gwas Of The Feral Horses (Equus Caballus) Of Theodore Roosevelt National Park, Melissa Amy Thompson

Theses and Dissertations

Theodore Roosevelt National Park (TRNP) is home to a herd of feral horses (Equus caballus) which were present on the landscape prior to the establishment of the park and are now maintained as a living history demonstration herd. I used genomic analyses to investigate the TRNP horses’ relatedness to other breeds and the genetic basis for variation in response to a contraceptive vaccine. DNA from 118 horses was genotyped for 70k SNPs spread evenly across the genome. To clarify the relationship of this herd with other horses, I used population genomic analyses to compare the TRNP genotypes to a dataset …

Houghton Mifflin Harcourt Journeys Curriculum Series Longitudinal Effects On Grade 4 Student Reading Scores, Daniel Warcken

Theses and Dissertations

Within the last 100 years, the number of school districts in America has dropped by as much as 90% (Murdock, 2012), from 117,108 in 1939 to 13,452 in 2019 (National Center for Education Statistics [NCES], 2020). Funding and how to provide educational equity, equality, and justice for all is a critical conversation. According to the North Dakota Department of Public Instruction (NDDPI) (2018), 1.94% of the state’s $1.448 billion cumulative K-12 expenditures are associated with instructional media related to academic aids such as textbooks.

The purpose of this quantitative study was to identify if the major educational publishing company Houghton …

Development Of Biomass-Derived Nanoparticles For Biological And Medical Applications, Sarah Elizabeth Reagen

Theses and Dissertations

Quantum dots (QDs) are a subset of nanoparticles (NPs) that have several unique optical and electrical properties that make them viable for fluorescent cell labeling applications. However, these QDs in the past were composed of toxic heavy metals such as lead and cadmium, and therefore, graphene-based QDs (GQDs) have been examined as an alternative material for these cell labeling applications for their increased biocompatibility. Furthermore, GQDs can produced singlet oxygen (1O2) for photodynamic therapy (PDT), making them capable of being used as a PS agent for cancer treatment. Additionally, hollow mesoporous silica nanoparticles (hMSNs) are capable of being an alternative …

Telehealth Satisfaction Amidst The Covid-19 Pandemic, Hillary Smith

Theses and Dissertations

The COVID-19 pandemic caused substantial disruptions and changes in mental health service delivery as community mental health clinics had to scramble to implement telehealth services to provide continuity of care under quarantine. For the current study, I conducted in-depth video interviews with ten clients from a community mental health training clinic who transitioned from in-person services to telehealth services. Twenty-one factors related to clients’ satisfaction with telehealth services and their perceived barriers to the treatment modality using a grounded theory framework. The majority of participants reported they were satisfied with telehealth but preferred to return to in-person therapy sessions with …