Digital Commons Network^™

Exploring The Role Of Rb1 In Cellular Differentiation Using Patient-Derived Stem Cells, Ambily Vincent

Theses and Dissertations

The Retinoblastoma (RB1) gene, predominantly recognized as a tumour suppressor, plays a crucial role in early growth and development. Mice lacking the retinoblastoma gene do not survive and exhibit defects in the differentiation of various tissues, underscoring its involvement in cellular differentiation. This suggests that the functions of the RB1 gene extend beyond its role as a tumour suppressor, encompassing cell cycle regulation, maintenance of genomic stability, cellular senescence, and cellular differentiation.

While the tumour suppressor function of the RB1 has been extensively studied, its role in cellular differentiation remains incompletely understood. Existing literature, primarily based on animal models, has …

Rna Sequencing Reveals Chemotherapy-Induced Changes In Genes Associated With Neurogenesis And Neuronal Function, Bailey Cronin

Theses and Dissertations

Over 310,000 new cases of breast cancer will be diagnosed in 2024, making it the most commonly diagnosed cancer in United States women. Advancements in treatment options such as chemotherapy and radiation have resulted in a 5-year survival rate of 91%. With nearly 4.1 million breast cancer survivors living in the United States today, there is a need for a better understanding of the long-term side effects of these treatments and the impact these may have on the quality of life for these survivors. Reported in up to 78% of cancer patients who received chemotherapy, chemotherapy induced cognitive impairment (CICI), …

Raising Hope: A Preliminary Investigation Of Hope-Strengthening Parenting, Anna Sellers Calley

Theses and Dissertations

Hope Theory defines hope as a cognitive-motivational process of seeking and obtaining goals through agency thinking and pathway building, with substantive connection to many desirable outcomes. While hope is theorized to begin developing early in life, this assumption has never been empirically investigated. This is due to limitations in established hope measurement. There is an additional gap in the research surrounding the role of parents in the development of hope. In this study, I address each of these gaps by utilizing a novel measure of hope in early childhood and running a multiple linear regression model to investigate potential connections …

Investigating Cloth Sculpting For Simulation Control In Virtual Reality, Matthew S. Bishop

Theses and Dissertations

Artists require control and predictability over physically based simulations to create their required animation. This control can lead to reduced iteration time to get the desired simulation results. Controlling cloth simulations can require manual effort to create goal keyframes for control algorithms to match. Current techniques and research utilize a keyboard and mouse to create these goal keyframes. Other research has looked at using a virtual reality (VR) head-mounted display with controllers to improve the experience of completing similar animation tasks. However, we are not aware of any research that attempts to use VR as a tool to assist artists …

An Agent-Based Modeling Of Permafrost Melting And Virus Exposure Due To Climate Change, Nishat Ara Mozid

Theses and Dissertations

Climate change has affected human life and natural system significantly. Particularly, there is specific evidence of change in the Arctic region for climate warming. One of the consequences of climate change is the process of permafrost degradation and thawing rate of active layer. Permafrost is defined as frozen grounds that exists at a temperature of or below 0℃ for at least two consecutive years, consists of ice and novel pathogens which are frozen for a long time. Anthrax may cause a serious threat for local environment and livestock due to permafrost melting. The study introduces an agent-based modeling of permafrost …

Potential Contributions Of Xenobiotics To P-Glycoprotein Clearance Of Amyloid Β-Peptide From The Brain, Joseph Jr Asante

Theses and Dissertations

Alzheimer’s disease (AD) is an example of a neurodegenerative disease, mostly age related and characterized by cognitive decline. Currently there is no effective treatment that can prevent or slow progression of the disease. Roughly 95% of cases are sporadic, with unknown etiology. A distinguishing characteristic of the Alzheimer’s brain is the presence of amyloid plaques, with their constituent amyloid-β (Aβ) peptides implicated in causing synaptic dysfunction, memory impairment, and neurotoxicity. Although extensive research has explored the role of Aβ peptides in AD, understanding their metabolism in health versus disease remains insufficient. P-glycoprotein (P-gp), a member of the ATP-binding cassette transporter …

Spending The Peace Dividend: Nato And The Failures Of American Diplomacy, Caleb Batman

Theses and Dissertations

Recent years have seen a massive increase in available resources pertaining to US-Russian diplomacy in the years immediately following the collapse of the Soviet Union. Using these newly available resources including diplomatic cables, declassified transcripts, and personal correspondence between President Clinton of the United States and President Yeltsin of the Russian Federation the author attempts to illustrate how the White House missed a chance for reconciliation with Russia during the first Clinton term. Additionally, this paper illustrates how it was not merely bad diplomacy that led to a resumption of Cold War hostilities, but willful policy in Washington that sought …

Caring Until It Hurts: A Survey Of Compassion Fatigue And Moral Injury Among Wild Animal Caregivers And Its Implications For Animal Welfare In Zoos, Aquariums, Rehabilitation Centers, And Sanctuaries, Nadiya L. Delgado

Theses and Dissertations

Caregivers for captive wild animals are fundamental to the overall care, welfare, and conservation of animals in their charge, fulfilling diverse and critical roles. Caring for others, whether humans or animals, exposes caregivers to compassion fatigue – a state of emotional and physical exhaustion resulting from prolonged empathetic engagement with those they assist, and moral injury – psychological distress that arises from actions, or the lack of them, which violate one's moral or ethical code. This distress can manifest as intense feelings of guilt, shame, or a sense of betrayal by oneself or others. These phenomena are pertinent in environments …

The Effects Of People, Time Of Day, And Season On Affiliative And Stereotypic Behaviors In Captive Rhinoptera Bonasus And Other Rays Across Two Human-Interactive Enclosures, Wren C. A. Newman

Theses and Dissertations

Cownose rays (Rhinoptera bonasus) are among the most common species found in aquaria, yet the impact of touch enclosures on their welfare remains largely unknown. While research on animal-visitor interactions has produced mixed results for other species, the lack of studies focusing on fish welfare poses a risk of overlooking potential welfare concerns. Therefore, we aimed to establish a baseline for cownose rays regarding stereotypic behaviors, sociality, and activity in captivity, and to examine the influence of human interaction on these behaviors. We collected over 700 hours of footage from two enclosures at Adventure Aquarium in Camden, NJ …

Sex Differences In The Diets Of Forest-Dwelling Baboons (Papio Anubis), Kara Hartunian

Theses and Dissertations

The ability to eat and switch between many food sources is an adaptative feeding strategy utilized for meeting nutritional needs across different environments, seasons, and physiological conditions. Baboons (genus Papio) excel at both dietary flexibility and foraging selectively, and consequently have one of the most widespread geographic distributions in the Order. To further expand to our understanding of baboon feeding ecology, I analyzed foraging activity and the nutritional characteristics of the diets of the relatively understudied rainforest baboons (Papio anubis) living in Kibale National Park, Uganda. I also investigated if there are differences in food type choice …

Assessment Of Tripeptides Self-Assembly & Er Stress On Cell Viability And Exosome Secretion In Mda-Mb231, Azmat Parveen

Theses and Dissertations

This study delves into the effects of tripeptides KYpF and WYpK(NBD) on MDA-MB-231 cells, uncovering KYpF's ability to reduce cell proliferation and exosome secretion in peptide treated cells. Conversely, WYpK(NBD) demonstrated significant toxicity at 10 μM. These insights pave the way for optimizing peptide-based cellular treatments for exosome secretion purposes.

Maternal And Other Social Influences On Signature Whistle Structure Of A Captive Group Of Bottlenose Dolphins (Tursiops Truncatus), Riley Synan

Theses and Dissertations

Bottlenose dolphins (Tursiops truncatus) are social animals that use acoustics as a major method of communicating across a marine environment. They also live in fission-fusion groups and thus interact with many conspecifics over a lifetime. Individual dolphins have a signature whistle that functions as an identity call and serves to maintain group cohesion in murky waters or during reconciliation. Dolphins exhibit vocal learning and the signature whistle of a calf may be modeled after the whistle of their mother or another individual in the pod as they develop. Mother-calf pairs or other related animals sometimes have similar whistle types. Some …

Assessing Comprehension And Competence In Bottlenose Dolphins (Tursiops Truncatus) On The Concept: Do Something Different, Charlotte M. Mulligan

Theses and Dissertations

Many studies have been conducted using a training paradigm based on the research of Pryor et al. (1969) wherein the participants are trained on the abstract concept: do something different. This paradigm challenges working memory and requires the participants to respond with a behavior, either previously trained or novel, which has not yet been offered within the session. The work of Braslau-Schneck (1994) expanded this concept and demonstrated the ability of dolphins to successfully complete this task in synchronized pairs, though the method of accomplishing this task remained unclear. The present research was conducted as part of a broader …

Enhancing The Visual Environment Of Indoor Housed Laboratory Cynomolgus Macaques (Macaca Fascicularis) To Reduce Stress And Maladaptive Behavior, Jeannine C. Rodgers

Theses and Dissertations

Nonhuman primates housed in indoor laboratory environments are generally exposed to nondescript background visual stimuli. This environment is not ideal for species whose primary sensory modality is visual. To provide a more naturalistic visual environment that would potentially improve their welfare, this study assessed the efficacy of a passive natural visual stimulus in the reduction of stress and stereotypy and agonistic behaviors in a cohort of four (2 male, 2 female) Cynomolgus macaques (Macaca fascicularis) housed in an indoor laboratory facility in New York City. The macaques were exposed to four different videos depicting scenes of natural landscapes that were …

The Effect Of The Lunar Cycle On The Demographics Of Asian Elephants Observed In Open Areas In A Protected Wildlife Sanctuary And Surrounding Crop Fields, Serene K. Rivera

Theses and Dissertations

Due to human development and habitat fragmentation, Asian elephants increasingly use human landscapes and encounter people. These interactions have led to negative interactions between humans and elephants, such as crop-raiding. Despite efforts to mitigate these conflicts, farmers have not been able to consistently protect their crops from elephant foraging. Therefore, it is important to understand elephant behavior in these landscapes to help create long term mitigation plans. Specifically, research regarding demographic patterns in Asian elephants’ perceived predation risk can inform wild elephant management. This study used camera traps in Salakpra Wildlife Sanctuary in Kanchanaburi, Thailand to observe the wild Asian …

Envelope Of Ruin : Material And Spatial Responses Of New York City Homeless To The Covid-19 Pandemic, Kyle C. Maxey

Theses and Dissertations

This paper attempts to understand human material responses to catastrophic urban collapse. Using Henri Lefebvre's theories of spatial production and the history of a section of the Brooklyn-Queens Expressway (BQE) this paper develops a theoretical framework for understanding the dynamics present in the creation of an urban ruin. Through the observation of homeless material responses, particularly the construction of encampments under the BQE, during the COVID-19 pandemic "lock-down" in New York City, this thesis illustrates how populations respond to urban ruin and social collapse. Moving forward, this paper hopes to serve as a synthesis of urban theory and archeological observation …

Narcissus Goes Digital: The Impact Of Zoom’S Self-View Feature On Cognitive Load And Zoom Fatigue Among Those With High And Low Adhd Symptoms, Abigail Aris Weir

Theses and Dissertations

Individuals with ADHD may be more vulnerable to an increased cognitive load during video conferencing (e.g., Zoom) due to the distracting nature of their reflection being displayed by the self-view feature. This feature, however, may also provide stabilizing effects by offering consistent visual feedback for self-monitoring, thus reducing anxiety.

Fibersheds: Textiles As The Basis For A New World, Rebecca John

Theses and Dissertations

The Fibershed movement seeks to develop bioregional fiber systems to cultivate natural fibers through regenerative agriculture. Through this, it seeks to revive domestic textile production as an alternative to the global textile industry. This thesis investigates the Fibershed movement at national and regional scales, and assesses how they position themselves as an alternative economy.

Historical & Contemporary Practices Of Seal Harvesting: Policy Implications For Revitalization Among The Taa-Laa-Wa Dee-Ni’, Paula Lynne Aubrey

Theses and Dissertations

This dissertation explores the contemporary practices of seal harvesting among Indigenous Peoples, with a special focus on policies that inform the revitalization of seal harvesting within the Taa-laa-waa Dee-ni’ (Tolowa) community of Northern California and Southern Oregon. The research is conducted by an Indigenous researcher seeking to further connect to traditional practices of her tribe, and this research places a strong emphasis on cultural sensitivity and ethical integrity. The study involves a dual methodology including a comprehensive literature review to understand the cultural and ecological significance of seal harvesting, and a policy artifact review to identify wise practices and policy …

Experimental Investigation Of The Suitability Of Soybean-Derived Surfactant (Sods) In Gas Well Deliquification, Abiodun Gbadegesin Adeyemi

Theses and Dissertations

Gas well deliquification is a crucial process in the oil and gas industry, focused on maintaining or restoring production rates by removing liquids that impede gas flow. This is a recurring challenge that is prominent in many gas wells across North Dakota. Surfactants play a vital role in this process by altering the interfacial tension between gas and liquid phases, alleviating liquid accumulation in these wells. However, due to their high production costs, non-biodegradability, and toxicity, the use of synthetic surfactants has raised environmental concerns. The pursuit of sustainable and environmentally friendly alternatives has led to the exploration of natural …

High Performance Simulation Of Astrocyte Morphology And Dynamics For Brain Imaging, Lun Zhao

Theses and Dissertations

The brain is a complex organ composed of neurons and glial cells. Historically, glial cells were often overlooked in neuroscience research, but their significance has gained increasing recognition in recent years due to mounting evidence demonstrating their pivotal roles in brain function and disease. Notably, a growing body of research highlights the active involvement of astrocytes, the most abundant type of glial cells, in numerous neurological diseases. With the development of microscopy technology, our understanding of astrocytes is becoming more and more abundant. Due to the characteristics of microscopy, tissue scattering and environmental noise, there is a high demand for …

Transcriptional Landscape Of Foxp3+ Regulatory T Cells Under Mitochondrial Oxidative Stress: Implications For Inflammaging, Jappreet Singh Gill

Theses and Dissertations

This thesis explores the intricate relationship between mitochondrial oxidative stress and the function of FOXP3+ regulatory T cells (Tregs), highlighting their essential roles in the aging immune system and the development of age-related diseases. The study delves into how mitochondrial impairments within Tregs contribute to widespread inflammation leading to pathological aging. One key analysis shows that mitochondrial oxidative stress undermines Treg functionality, intensifying inflammatory responses and increasing frailty in older adults. This dysfunction is associated with elevated inflammatory markers and presents a potential target for therapeutic interventions aimed at restoring mitochondrial health to alleviate these impacts. Another significant discovery connects …

Adaptation To The Impacts Of Climate Change On Water Availability In The Upper Red River Of The North (Urrn), Innocent Kelechi Anosike

Theses and Dissertations

In the Upper Red River of the North (URRN), climate change has appeared to impact water availability, causing several major floods in recent years, and raising questions about whether climate change is responsible for the frequent occurrence of flooding in this major agricultural region in the Midwest USA. This thesis centers on investigating adaptative measures to mitigate the impacts of climate change in the URRN. One of the methods used is to compare changes in the outcomes of flood frequency analysis (FFA) for “before (pre-industrial) and after (industrial)” time periods. The study uses a combination of data analysis and literature …

Optimizing Laser-Based Wireless Power Transfer: A Comprehensive Study Of A 4 Quadrant Pv Module System And Its Powering And Tracking Capabilities, Ahmed Elfarran

Theses and Dissertations

This research investigates laser-based tracking systems for wireless power transfer (WPT) applications. Beginning with an overview of renewable energy and WPT technologies, the study establishes both theoretical frameworks and practical methodologies for laser-based power transmission. The experimental analyses encompass load testing with a four-quadrant module, examining the impact of laser power, load conditions, and beam diameter on power transfer efficiency, as well as the influence of target movement speed on voltage stability. Notably, we achieved a tracking speed of up to 20 mm/s, ensuring efficient power delivery even with target motion. The results demonstrate clear correlations between laser power levels …

Maturational Changes In The Epigenome And Transcriptome Of Hypothalamic Astrocytes: Implications For Axonal Regeneration, Abiodun Ebenezer Odufuwa

Theses and Dissertations

Axon regeneration is essential for effective repair following injury in the CNS. One of the burdens of aging is the lack of recovery following injury in the CNS. Our previous studies show that in 35-day-old rats, axon regeneration occurs in response to a unilateral hypothalamic lesion in the hypothalamus neurohypophyseal system. However, this regenerative ability is lost in 125-day-old rats. Furthermore, treatment of hypothalamic organotypic culture with ciliary neurotrophic factor (CNTF) promotes axonal outgrowth and survival. Our previous studies suggest that astrocytes may be playing critical roles in this maturational loss of regenerative capacity because only astrocytes expressed all the …

Opencast: Cyanobacteria Growth And Correlations With Predictive Factors In Creation For Harmful Algal Bloom Formation, Devarshi Nikhil Patel

Theses and Dissertations

Harmful Algal Blooms (HABs) are a significant threat to aquatic ecosystems, public health, and local economies due to their production of toxins and negative impacts on water quality. This thesis investigates the correlations between cyanobacteria growth and environmental variables, including wind conditions, surface temperatures, and nutrient levels. This research uses machine learning and statistical modeling to predict HAB occurrences by analyzing these variables' historical and near real-time data. The findings indicate that wind speed, surface temperature, and nutrient levels influence cyanobacteria dynamics, with specific thresholds identified for predictive accuracy. The developed HAB predictive models and the graphical user interface (GUI) …

An Analysis Of Fish Assemblages And Flow Regimes Upstream And Downstream Of Falcon Reservoir, Christopher J. Austin

Theses and Dissertations

The lower Rio Grande is regulated by Falcon and Amistad dams. Native fish species rely on the natural flow regime for habitat and spawning. I analyzed streamflow discharge and fish assemblages above and below Falcon Reservoir to assess potential impacts of dam impoundment. Fish collections were conducted by seine, and river discharge data was provided by the International Boundaries and Water Commission. I found significant differences in fish assemblages above vs below Falcon Reservoir, and the flow regime was significantly more altered downstream of Falcon Reservoir. I also identified two Species of Greatest Concern for Texas above Falcon Reservoir (Notropis …

Documenting The Spatial And Temporal Dynamics Of Pair-Rule Genes In The Early Tribolium Embryo, Hasan Mahmud

Theses and Dissertations

Although Insects and vertebrates have different anatomical structures, they possess similar mechanisms for segmenting during embryogenesis. Clock and wavefront model exhibit a similar pattern of division as observed in Drosophila, like vertebrates, which emphasizes fundamental principles in the field of developmental biology. In contrast to Drosophila, the insect Tribolium castaneum exhibits posterior segmentation originating from the growth zone. The pair-rule genes’ expression is characterized by oscillatory patterns, which are controlled by a segmentation clock. Researchers found that the speed regulation model combines oscillatory and sequential gene activities to shape both elongating and non-elongating embryonic tissues. The adaptability of this model …

Chemical Investigation Of The Ethanol Extract Of Tillandsia Usneoides (Spanish Moss), Jakir Hossain

Theses and Dissertations

Tillandsia usneoides in epiphytic bromeliads takes up water through absorptive trichomes on the shoot surface under extreme environmental conditions. Although previous studies revealed the way by which T. usneoides absorbs water and prevents water loss, its water transport remains unclear. Uses of T. usneoides L. have been documented going back to pre-Columbian and late archaic societies. Between the 1800s and 1900s. T. usneoides was utilized in fruit packing and polish, among other industrial goods. In terms of medicine, tillandsia is well-known for treating leucorrhea, rheumatism, ulcers, hemorrhoids, and as an anti-diabetic, emetic, analgesic, purgative, antispasmodic, and diuretic. The chemical composition …

Label-Free Laser-Induced Graphene-Based Electrochemical Biosensor Modified With Graphene-Conductive Polymer Ink Coating For Detection Of Alpha-Fetoprotein And 17Β – Estradiol, Ridma Tabassum

Theses and Dissertations

In this study, a novel electrochemical biosensor was proposed based on inducing graphene formation on polyimide substrate via laser engraving. Graphene polyaniline (G-PANI) conductive ink was synthesized by planetary mixing and applied to the working zone of the developed sensor to effectively enhance the electrical signals. The laser-induced graphene (LIG) sensor was used to detect alpha-fetoprotein (AFP) and 17β-Estradiol in the phosphate buffer saline (PBS) buffer and human serum. The electrochemical performance of the biosensor in determining these biomarkers was investigated by differential pulse voltammetry (DPV) and chronoamperometry (CA). In a buffer environment, alpha-fetoprotein (AFP) and 17β-Estradiol detection range were …