Digital Commons Network^™

Prevalence Of Campylobacter Jejuni In Newly Constructed Broiler Houses, Krista Nicole Eberle

Theses and Dissertations

Campylobacter is the leading cause of bacterial gastroenteritis in the United States. The objective of this study was to investigate the prevalence of Campylobacter in newly constructed broiler houses and compare three microaerophilic gas delivery methods used to culture Campylobacter in the laboratory. Of 2,300 litter, 900 fecal, and 45 water samples, only 5, 6 and 1 of the samples, respectively, were confirmed positive. style='mso-spacerun:yes'> Results indicated litter moisture content was different across day, location and house. An interaction was detected for litter pH between day, location and flock. Temperatures averaged 26.8°C inside and 27.6°C outside. style='mso-spacerun:yes'> No difference in …

Effects Of Timber Harvesting On Upland Oak Forests In The Missouri Ozarks, Qi Yao

Theses and Dissertations

Oak decline-induced mortality and failure of oak regeneration have become a concern in upland oak forests in the Missouri Ozarks. This project investigated the effects of timber harvesting on both oak regeneration and mortality of oak residuals following a suite of harvesting treatments in the Missouri Ozarks based on the sixteen-year monitoring data from the Missouri Ozark Forest Ecosystem Project (MOFEP). On dry sites by year 10, clearcutting improved the density of oak reproduction the most, and that intermediate cutting and clearcutting increased the proportion of black oak reproduction with 2% and 3%, respectively in the composition of the forests …

Understanding Molecular Aspects Of Catfish-Pathogen Interactions, Pradeepkumar Reddy Dumpala

Theses and Dissertations

The catfish industry suffers losses primarily due to enteric septicemia of catfish and columnaris disease caused by Edwardsiella ictaluri and Flavobacterium columnare, respectively. Understanding the host-pathogen interactions is vital for prevention and eradication of these diseases. Hence, the overall objective of this study was to analyze whole cell proteomes of these two bacteria, and to determine the changes in E. ictaluri protein expression against in vitro iron-restriction and host serum treatment. High-throughput proteomic analysis of these bacteria was conducted using two-dimensional liquid chromatography followed by electrospray ionization tandem mass spectrometry (2-D LC ESI MS/MS) and two-dimentional gel electrophoresis coupled with …

Temporal And Spatial Patterns Of Fish Distribution And Diversity In The Noxubee River, Mississippi And Alabama, Michael Thomas Calloway

Theses and Dissertations

The Mobile Basin is a prime example of a system that has undergone extensive channel modification with corresponding declines in the distribution and abundance of the native aquatic fauna. However, many of the declining aquatic species of the Mobile basin may persist within unmodified subbasins. The Noxubee River is a subbasin of the Mobile basin that has had very little alteration throughout its watershed. I investigated the species richness and assemblage structure to determine if the contemporary fish assemblage attributes resembled the conditions represented by historic collections. The findings of this study are important because the Noxubee River has not …

The Conceptual Ecology And Management Of Parrotfeather [Myriophyllum Aquaticum (Vell.) Verdc., Ryan Michael Wersal

Theses and Dissertations

Parrotfeather [Myriophyllum aquaticum (Vellozo) Verdecourt] is a non-native aquatic plant from South America that was introduced into the United States in the 1890's. Research was conducted to elucidate seasonal life history, starch allocation patterns, and key environmental factors that may affect plant growth. Environmental factors identified in field studies were used to develop a conceptual model to display relationships between growth and environmental factors. The conceptual model served as a broad-based hypothesis to parameterize growth limiting factors as it related to M. aquaticum growth. Mesocosm experiments were then conducted to test relationships depicted in the model and define the growth …

In Vivo Comparison Of Edwardsiella Ictaluri Survival In Kidneys Of Vaccinated And Naïve Rag1-/- Zebrafish, Casey Janine Varner

Theses and Dissertations

This study used rag1-/- mutant zebrafish, which lack functional T and B lymphocytes, to investigate whether innate immune cells from vaccinated mutant zebrafish demonstrate enhanced survival compared to phagocytes from naïve mutant fish. Edwardsiella ictaluri, an economically significant aquatic pathogen and the causative agent of enteric septicemia of catfish (ESC), was used for the trials. Quantification of live bacteria from sampled kidneys was accomplished via colony counts, luminescence readings, and differential DNA extractions using Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) followed by qPCR. There was a general trend of less bacteria in vaccinated mutant fish. Additionally, the mortality in …

Management Strategies For Glyphosate-Resistant Italian Ryegrass [Lolium Perenne L. Ssp. Multiflorum (Lam.) Husnot], Robin C. Bond

Theses and Dissertations

Glyphosate-resistant (GR) Italian ryegrass has been documented in many different countries around the world and has now become a major problem in row crop production areas of Mississippi. Field experiments were conducted from 2006 to 2008 in the Mississippi Delta to evaluate various herbicide and tillage treatment programs for its control. style='mso-spacerun:yes'> Highest level of control and reduction of GR Italian ryegrass biomass was observed with mechanically incorporated as well as surface applied residual herbicides in the fall of the year. style='mso-spacerun:yes'> Control of GR Italian ryegrass was 86-95% with surface applications of clomazone at 0.56, 0.84, and 1.12 kg …

Mode Of Action And Characterization Of A Novel Biological Response Modifier Isolated From Fractionated Caprine Serum, Charles Joseph Matyi

Theses and Dissertations

Immune Cell Potentiating Factor (ICPF) represents a class of biological response modifiers initially only found within active caprine serum fractions. Controlled studies have since demonstrated active ICPF derived from several non-caprine mammalian sources; including equine and human. ICPF is able to increase survivability in murine gram negative induced sepsis (60%) as well as secondary infection and subsequent sepsis in canines infected within canine parvo virus 2 (36%) despite showing no innate antiviral properties. ICPF is able to initiate systemic proteomic changes within several organ systems; including serum, liver, brain, lung, and spleen. ICPF initiated an early acute phase response, specifically …

Fire Ant Response To Management Of Native Grass Field Buffers, Sarah Lucinda Hale

Theses and Dissertations

Imported fire ants are invasive and cause injury to people, crops, livestock and wildlife. Disturbance may increase abundance and activity of fire ants. However, native grass field buffers established for grassland birds require periodic disturbance. I experimentally tested if fire ant mound density and foraging activity changed after burning and disking in native grass buffers and examined relationships among fire ants, vegetation, and grassland bird and butterfly metrics in undisturbed buffers. In 2008, disking increased mound density and foraging activity, but burning did not. In 2009, disking had no effect, but effects of disking the previous season persisted. Fire ant …

Evaluation Of In Vitro Serial Antibiotic Elution From Meropenem-Impregnated Polymethylmethacrylate Beads After Ethylene Oxide Gas And Autoclave Sterilization, Leonardo Alfredo Baez

Theses and Dissertations

The objective of this study was to determine elution properties of meropenem and compare the elutions of meropenem impregnated PMMA beads without sterilization (PMMA-Cont) to those sterilized with steam (PMMA-Auto) and ethylene oxide gas (PMMA-EO). Four groups of beads were produced: one group without antibiotic and three groups of meropenem impregnated beads: PMMA-Cont, PMMA-Auto, and PMMA-EO. Antibiotic concentrations in eluent samples were determined using a microbiological assay at different time intervals. The microbiological assay resulted in no zone of inhibition at all time periods for the PMMA-Auto samples and the samples of PMMA without antimicrobial. The meropenem concentration on the …

Factors Affecting Ammonia Volatilization From Broiler Litter, Dana Mcgee Miles

Theses and Dissertations

Loss of ammonia from broiler litter degrades air quality, decreases litter fertilizer value, and can have negative health consequences for birds and their caretakers. Rates of NH3 emission from broiler houses are complicated by interrelated management and environmental factors such as air temperature, humidity, house style, ventilation rate,bird age, litter conditions, litter characteristics, and cleanout schedule. Wide variations inemission rates necessitate further investigation of litter characteristics and abatement techniques. The research was designed to clarify the impact of moisture effects that are critical to emissions for poultry litter, in conjunction with bedding type and temperature. Experiments were conducted on litter …

Factors Affecting Morphometrics And Epiphyseal Closure Of White-Tailed Deer, Emily Brooke Flinn

Theses and Dissertations

Factors affecting morphometrics and epiphyseal closure are important in understanding regional variation and growth of white-tailed deer (Odocoileus virginianus). I compared body and antler growth from birth to 3 years of age in captive, first-generation, male white-tailed deer from three regions with varying soil quality and deer morphometrics. I also determined gender and age effects on epiphyseal closure timing in captive white-tailed deer. I found regional morphological variation present in first-generation male deer, which may be caused by regional genetic variation or lingering maternal effects. Determining cause of regional morphological variation will require data collection through a second-generation of males …

A Comparison Of Experiential Learning Activities Available To Juniors And Seniors In Secondary Agriscience Education And Science Education Courses, Brittany Leigh Beasley

Theses and Dissertations

The purpose of this study was to determine if secondary agricultural education courses provide students with more experiential learning opportunities than other science-based courses in the high school curriculum. An ex post facto research design was employed for the study. A researcher created instrument was distributed to a sample of Alabama agriscience teachers (23), science teachers (35), and agriscience students (909). Based on the responses of 8 agriscience teachers, 12 science teachers, and 103 students, there was a statistically significant difference in the percentage of class time students spent participating in experiential learning activities. Teachers and students indicated that agriscience …

Variation In Sound Production Of The Blue Catfish, Ictalurus Furcatus., Zachary Ghahramani

Theses and Dissertations

The blue catfish, Ictalurus furcatus, is an invasive species introduced to Virginia in 1974 and is the largest catfish in the United States. Like other Ictalurids, they are capable of producing disturbance calls via stridulation of the pectoral spine. These sounds can be made in air and water, and catfish can be preyed upon by both aerial and underwater predators. I characterized these putative distress calls by recording them in air and in the fish’s natural habitat. Sounds exhibited a wide variation in acoustic parameters relative to fish ontogeny: larger fish produced higher amplitude sounds with lower frequency bands. Sweep …

Cannabinoid Receptors In The 3d Reconstructed Mouse Brain: Function And Regulation, Peter Nguyen

Theses and Dissertations

CB1 receptors (CB1R) mediate the psychoactive and therapeutic effects of cannabinoids including ∆9-tetrahydrocannabinol (THC), the main psychoactive constituent in marijuana. However, therapeutic use is limited by side effects and tolerance and dependence with chronic administration. Tolerance to cannabinoid-mediated effects is associated with CB1R adaptations, including desensitization (receptor-G-protein uncoupling) and downregulation (receptor degradation). The objectives of this thesis are to investigate the regional-specificity in CB1R function and regulation. Previous studies have investigated CB1Rs in a subset of regions involved in cannabinoid effects, but an inclusive regional comparison of the relative efficacies of different classes of cannabinoids to activate G-proteins has not …

Volumetric Growth Model Of Human Medulloblastoma In The Nude Mouse Cerebellum, Thomas Gavigan

Theses and Dissertations

Medulloblastoma is the most common brain tumor in children, accounting for 10-20% of primary central nervous system (CNS) neoplasms and approximately 40% of all posterior fossa tumors. It is a highly invasive embryonal neuroepithelial tumor that typically arises in the cerebellar vermis and has a tendency to disseminate throughout the CNS early in its course. The molecular mechanisms of the disease largely remain uncharacterized, as the clinical treatment is still associated with mortality and severe side effects. The development of a clinically relevant in vivo model is important not only to further understand the disease but also to provide a …

Effects Of Duration Of Proton Pump Inhibitor (Ppi) Therapy On Markers Of Bone Health In Men And Postmenopausal Women, Zarina Maria Pabin

Theses and Dissertations

This observational study compared bone health biomarkers, bone mineral density (BMD),dietary habits, and physical activity levels of men (n=31) and non-estrogen supplementing postmenopausal women (n=23) divided according to duration of proton pump inhibitor (PPI) therapy; more than 5 years (n=16), less than 5 years (n=15), and no PPI therapy (n=23). The shortest duration of PPI therapy was 2 months and the longest duration of PPI therapy was 25 years with a mean duration of 7.5 years. No significant differences were found between measures of spine BMD, urinary deoxypyridinoline (bone resorption), urinary calcium and magnesium, serum osteocalcin (bone formation), serum parathyroid …

Factors Affecting Gingival Excess, Altered Passive Eruption And Recession In The Mandibular Anterior And Premolar Sites, William Bohlen

Theses and Dissertations

Abstract FACTORS AFFECTING GINGIVAL EXCESS, ALTERED PASSIVE ERUPTION AND RECESSION IN THE MANDIBULAR ANTERIOR AND PREMOLAR SITES By William F Bohlen, D.M.D. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University. Virginia Commonwealth University, 2010 Major Director: Thomas Waldrop, DDS, MS Program director, Department of Periodontics, Virginia Commonwealth University AIM: The aim of this study was to determine the factors affecting gingival excess, altered passive eruption and recession. METHODS: 100 subjects were examined clinically and models of their mandible were fabricated. Demographic, periodontal and cast measurements were …

Klf2/Klf4 Double Knock-Out Mouse Embryos Show Cranial Bleeding With Endothelial Disruption Of The Primary Head Vein, Benjamin Curtis

Theses and Dissertations

Krüppel-like factors (KLFs) are a family of 3 Cys2/His2 zinc finger transcription factors with a diverse set of roles in cellular differentiation, cell cycle regulation, tumor suppression, erythropoiesis, angiogenesis, and other processes. During embryonic development, KLF2 has a role in vessel maturation. Adult conditional KLF4 knockout mouse embryos have thickened arterial intima follow vascular injury. Breeding KLF2+/- and KLF4+/- mice resulted in the generation of KLF2/KLF4 double knockout (DKO) embryos. KLF2/KLF4 DKO embryos died by E10.5 with cranial bleeding. Using immunohistochemistry, embryo whole-mounts were examined for differences in gross vascularization between wild-type (WT), KLF2-/- and KLF2/KLF4 (DKO embryonic day 9.5 …

Novel Roles For Reelin In Retinogeniculate Targeting, Cheryl Haner

Theses and Dissertations

In the developing visual system, the axon of a pre-synaptic cell must be guided to a post-synaptic partner. Retinal ganglion cells (RGCs) in the eye are an excellent model to study this process. Multiple classes exist that respond to specific types of light input, and these project to different destinations in the brain that process distinct types of information. The RGC axons that navigate to the lateral geniculate nucleus (LGN) do so in a class-specific manner. Axons from RGCs that mediate non-image forming functions innervate the ventral LGN (vLGN) and the intergeniculate leaflet (IGL). Axons from RGCs that process image-forming …

Tregtia Elektronike, Naim Neziri

Theses and Dissertations

Tregtia Elektronike (e-commerce) është shkëmbim virtual (paperless) i informacioneve

te biznesit duke përdorur EDI-(electronic data interchange), e-mail, buletinin elektronik në bord,

faks transmisionet dhe fondin elektronik te transfereve. Poashtu i referohet online stoqeve,

transaksioneve të bondeve , “shkarkimeve” dhe shitjeve të produktevete“buta”(software, muzikë

dokumenteve,etj) dhe transaksionve B2B- business to business. [1]

Tregtia elektronike ka pasur një impakt enorm në biznes kudo në botë andaj edhe qëllimi

kryesor i këtij studimi është që të informojë biznesin dhe konsumatroin kosovar me gjithqka

rreth asaj se si bëhet Tregtia elektronike.

Në ditët e sotme , Tregtia Elektronike, është duke e ndërruar mënyren e …

Prevention Of Spinal Anesthesia Induced Hypotension During Elective Cesarean Section, Keith Nelson

Theses and Dissertations

The incidence of cesarean delivery has increased dramatically over the past decade. According to the Center for Disease Control and Prevention's National Center for Health Statistics (NCHS), cesarean deliveries accounted for more than 30% of all births in 2005, setting a new United States record. Due to the increased risks, morbidity, and mortality associated with general anesthesia for cesarean sections, regional anesthesia is most often selected for non-emergent cesarean deliveries. Unless a preexisting labor epidural is in place, spinal anesthesia is most often the regional technique of choice for cesarean deliveries due to its immediate onset and dense blockade. Hypotension …

The Morphology Of Nouns In The Ugoroŋmo Language (Arara Of Pará), Shirley Dias Cardoso De Souza

Theses and Dissertations

In this thesis I describe the morphology of nouns in the Ugoroŋmo language (Arara of Pará), which is spoken by just over three hundred people. The Ugoroŋmo people live on the bank of the Iriri river, in two villages (Laranjal and Cachoeira Seca) in the state of Pará, Brazil.

The nouns of this language can be divided in three main groups: those that are not inflected for possession, those that may be inflected for possession, and those that must be inflected for possession (inherently possessed nouns). Possession is marked by two affixes: a possessor prefix and a possessive suffix. Inherently …

The Significance Of Gender In Literacy Instructions, Julie Kragness

Theses and Dissertations

No abstract provided.

Laptop Education: Impact On Learning And Achievement, Christopher E. Mills

Theses and Dissertations

The purpose of this study was to examine how 1:1 Laptop initiatives in the 10th, 11th, and 12th grade affected student achievement and student academic performance over the 2008–2009 school year at a selected small rural, Minnesota high school. The two variables in this study were: perceived student performance skills based on survey results from both student and teacher participants, and pre-existing Minnesota graduation testing results and grade point averages from Fertile-Beltrami School District students. The three questions that were researched in this study were: (1) What effects does a 1:1 Laptop initiative have on student academic performance based on …

Smokers' Responses To Fearful And Disgust-Eliciting Anti-Smoking Media, Mark C. Goetz

Theses and Dissertations

The purpose of this study was to build upon Wakefield and colleagues and Vogeltanz-Holm and colleagues hypotheses that anti-smoking ads are effective by means of eliciting negative emotional states, particularly disgust. In this study, we compared two sets of ads: those high in fear and disgust and those high in only fear. We hypothesized that subjective and physiological responses to ads high in fear and have a disgust component would be greater than for the fear-only ads. We also predicted that the ads high in disgust would have higher rates of recall and engagement. Last, we predicted that participants viewing …

A Phonological Description Of "Pet Talk" In Arara, Isaac Costa De Souza

Theses and Dissertations

The Arara people of Para, Brazil, as a whole, are remnants or survivors of some larger Cariban groups who descended from the headwaters of the upper Xingu to the mid and low areas of this river by the beginning of the nineteenth century. Now they live in three different villages: Maia, Cachoeira Seca and Laranjal.

The present thesis aims to describe thirteen different ludlings or "play languages" that elderly Arara people from Laranjal know and sometimes use in talking to pets. Play languages are linguistic forms that are purposely manipulated at some level. The strategies which the Arara people use …

The Impact Of Adolescent Nicotine Exposure On Drug Dependence In Adulthood, Mai Alajaji

Theses and Dissertations

Nicotine is one of the first and most commonly abused drugs in adolescence. According to The Center for Disease Control, every day more than 6000 adolescents try their first cigarette and over 3000 of them become daily smokers. Smoking among adolescents is a strong predictor of future drug abuse and dependence in adulthood. A number of studies has suggests that adolescents pre-exposed to nicotine may suffer permanent disruption of the brain’s reward systems through changes in dopamine receptor function. We hypothesize that nicotine exposure during adolescence causes long lasting neurobiological alterations that increase the likelihood of cocaine use in adulthood. …

Construction Of Complex A At La Venta, Tabasco, Mexico: A History Of Buildings, Burials, Offerings, And Stone Monuments, Arlene Colman

Theses and Dissertations

In 1969, Paul Tolstoy commented that archaeological investigation at La Venta had become "a fairly long and at times tortuous story of excavation, interpretation, re-interpretation, and depredation at the famous site found by Stirling." This thesis adds to the torture by describing and illustrating the architecture, burials, offerings, and stone sculpture of La Venta Complex A in an effort to reconcile data into an accurate sequence of meaningful cultural events. The details derive from excavation reports, field notes, maps, photographs, and correspondence of the early investigators of the site. This study addressed three myopic perceptions regarding La Venta: (1) the …

Interactions Of Burkholderia Pseudomallei And Acanthamoeba Castellanii And Their Effects On Virulence In Human Monocytes, Emily Ann Moore

Theses and Dissertations

Burkholderia pseudomallei, the etiological agent of melioidosis, is a saprophytic bacterium existing endemically in the water and soil of SE Asia and Northern Australia. This organism has shown the ability to remain dormant in its host for decades. B. thailandensis is a closely related non-pathogenic near neighbor that is also found in these soils. It has been suggested that free-living amoeba could be natural reservoirs for these organisms. The interactions of Burkholderia species and Acanthamoeba castellanii, a species of free-living amoeba, were studied to better understand the natural ecology of these organisms and to determine the effects amoeba …