Digital Commons Network^™

Guidance For Management Of Free-Roaming Community Cats: A Bioeconomic Analysis, Valeria A. Benka, John D. Boone, Philip S. Miller, Joyce R. Briggs, Aaron M. Anderson, Christopher Slootmaker, Margaret Slater, Julie K. Levy, Felicia B. Nutter, Stephen Zawistowski

Publications and Research

Objectives This study used computer simulation modeling to estimate and compare costs of different free-roaming cat (FRC) management options (lethal and non-lethal removal, trap–neuter–return, combinations of these options and no action) and their ability to reduce FRC population abundance in open demographic settings. The findings provide a resource for selecting management approaches that are well matched for specific communities, goals and timelines, and they represent use of best available science to address FRC issues.

Methods Multiple FRC management approaches were simulated at varying intensities using a stochastic individual- based model in the software package Vortex. Itemized costs were obtained from …

Structure Of A Monomeric Photosystem Ii Core Complex From A Cyanobacterium Acclimated To Far-Red Light Reveals The Functions Of Chlorophylls D And F, Christopher J. Gisriel, Gaozhong Shen, Ming-Yang Ho, Vasily Kurashov, David A. Flesher, Jimin Wang, William H. Armstrong, John H. Golbeck, Marilyn R. Gunner, David J. Vinyard, Richard J. Debus, Gary W. Brudvig, Donald A. Bryant

Publications and Research

Far-red light (FRL) photoacclimation in cyanobacteria provides a selective growth advantage for some terrestrial cyanobacteria by expanding the range of photosynthetically active radiation to include far-red/near-infrared light (700–800 nm). During this photoacclimation process, photosystem II (PSII), the water:plastoquinone photooxidoreductase involved in oxygenic photosynthesis, is modified. The resulting FRL-PSII is comprised of FRL-specific core subunits and binds chlorophyll (Chl) d and Chl f molecules in place of several of the Chl a molecules found when cells are grown in visible light. These new Chls effectively lower the energy canonically thought to define the “red limit” for light required to drive photochemical …

Insights Into Using Plants In Management Of Viral Diseases., Vyacheslav Dushenkov, Anna Dushenkov

Publications and Research

Botanical therapeutics possess a unique potential in the treatment of viral diseases. The pharmacological base and clinical use of botanical therapeutics have been extensively reviewed. The mode of action(s) may be built either on the direct interference with the virus's ability to enter human cells, virus replication, or exerting immune-modulatory and anti-inflammatory effects. The adjuvant treatment with botanical therapeutics has the potential to result in advances in symptom resolution, decrease in disease burden and shortening its duration.

Me3al-Mediated Domino Nucleophilic Addition/Intramolecular Cyclisation Of 2-(2-Oxo-2-Phenylethyl)Benzonitriles With Amines; A Convenient Approach For The Synthesis Of Substituted 1-Aminoisoquinolines, Krishna M. S. Adusumalli, Lakshmi N.S. Konidena, Hima B. Gandham, Krishnaiah Kumari, Krishan R. Valluru, Satya K.R. Nidasanametla, Venkateswara R. Battula, Hari K. Namballa

Publications and Research

A simple and efficient protocol for the construction of 1-aminoisoquinolines was achieved by treating 2-(2-oxo-2- phenylethyl)benzonitriles with amines in the presence of Me 3 Al. The reaction proceeds via a domino nucleophilic addition with subsequent intramolecular cyclisation. This method provides a wide variety of substituted 1-aminoisoquinolines with good func- tional group tolerance. Furthermore, the synthetic utility of this protocol was demonstrated in the successful synthesis of the anti- tumor agent CWJ-a-5 in gram scale.

Type Vi Secretion System Mutations Reduced Competitive Fitness Of Classical Vibrio Cholerae Biotype, Benjamin Kostiuk, Francis J. Santoriello, Laura Diaz-Satizabal, Fabiana Bisaro, Kyung-Jo Lee, Anna N. Dhody, Daniele Provenzano, Daniel Unterweger, Stefan Pukatzki

Publications and Research

The gram-negative bacterium Vibrio cholerae is the causative agent of the diarrhoeal disease cholera and is responsible for seven recorded pandemics. Several factors are postulated to have led to the decline of 6th pandemic classical strains and the rise of El Tor biotype V. cholerae, establishing the current 7th pandemic. We investigated the ability of classical V. cholerae of the 2nd and 6th pandemics to engage their type six secretion system (T6SS) in microbial competition against non-pandemic and 7th pandemic strains. We report that classical V. cholerae underwent sequential mutations in T6SS genetic determinants that initially exposed 2nd pandemic strains …

The Temperature-Dependent Conformational Ensemble Of Sars-Cov-2 Main Protease (Mpro), Ali Ebrahim, Blake T. Riley, Desigan Kumaran, Babak Andi, Martin R. Fuchs, Sean Mcsweeney, Daniel A. Keedy

Publications and Research

The COVID-19 pandemic, instigated by the SARS-CoV-2 coronavirus, continues to plague the globe. The SARS-CoV-2 main protease, or M^pro, is a promising target for development of novel antiviral therapeutics. Previous X-ray crystal structures of M^pro were obtained at cryogenic temperature or room temperature only. Here we report a series of high-resolution crystal structures of unliganded M^pro across multiple temperatures from cryogenic to physiological, and another at high humidity. We interrogate these datasets with parsimonious multiconformer models, multi-copy ensemble models, and isomorphous difference density maps. Our analysis reveals a temperature-dependent conformational landscape for M^pro, including …

Changes In Histone H3 Acetylation On Lysine 9 Accompany Aβ 1-40 Overexpression In An Alzheimer’S Disease Yeast Model, Muna M. Hugais, Samantha N. Cobos, Seth A. Bennett, Jailene Paredes, Genevieve Foran, Mariana P. Torrente

Publications and Research

Alzheimer’s Disease (AD), the most common type of dementia, is a neurodegenerative disease characterized by plaques of amyloid-beta (Aβ) peptides found in the cerebral cortex of the brain. The pathological mechanism by which Aβ aggregation leads to neurodegeneration remains unknown. Interestingly, genetic mutations do not explain most AD cases suggesting that other mechanisms are at play. Epigenetic mechanisms, such as histone post-translational modifications (PTMs), may provide insight into the development of AD. Here, we exploit a yeast Aβ overexpression model to map out the histone PTM landscape associated with AD. We find a modest decrease in the acetylation levels on …

Vhs Archives, Committed Media Praxis, And ‘Queer Cinema', Alexandra Juhasz

Publications and Research

Committed media praxis is a doing as much as it is a knowing. Queerness is a manner of being as much as it is a politics, theory, or set of modish objects. This chapter about topics that are also processes—queer, media praxis, cinema—performs these across two acts: “Part 1: A Hesitant or Maybe Just Slightly Defiant Preamble,” is a creative unfolding, in the body of the text and as much so in its footnotes, of the author’s “queer feminist media praxis”: “Part 2: VHS Archives” is a demonstration of VHS Archives, a multi-sited, many-yeared project in experimental pedagogy, web-based archival …

Late Pleistocene And Early Holocene Sea-Level History And Glacial Retreat Interpreted From Shell-Bearing Marine Deposits Of Southeastern Alaska, Usa, James F. Baichtal, Alia J. Lesnek, Risa J. Carlson, Nicholas S. Schmuck, Jane L. Smith, Dennis J. Landwehr, Jason P. Briner

Publications and Research

We leverage a data set of >720 shell-bearing marine deposits throughout southeastern Alaska (USA) to develop updated relative sea-level curves that span the past ~14,000 yr. This data set includes site location, elevation, description when avail-able, and 436 14C ages, 45 of which are published here for the first time. Our sea-level curves suggest a peripheral forebulge developed west of the retreating Cordilleran Ice Sheet (CIS) margin between ca. 17,000 and 10,800 calibrated yr B.P. By 14,870 ± 630 to 12,820 ± 340 cal. yr B.P., CIS mar-gins had retreated from all of southeastern Alaska’s fjords, channels, and passages. At …

Cryo-Em Structure Of Mechanosensitive Channel Ynai Using Sma2000: Challenges And Opportunities, Claudio Catalano, Danya Ben-Hail, Weihua Qiu, Paul Blount, Amedee Des Georges, Youzhong Guo

Publications and Research

Mechanosensitive channels respond to mechanical forces exerted on the cell membrane and play vital roles in regulating the chemical equilibrium within cells and their environment. Highresolution structural information is required to understand the gating mechanisms of mechanosensitive channels. Protein-lipid interactions are essential for the structural and functional integrity of mechanosensitive channels, but detergents cannot maintain the crucial native lipid environment for purified mechanosensitive channels. Recently, detergent-free systems have emerged as alternatives for membrane protein structural biology. This report shows that while membrane-active polymer, SMA2000, could retain some native cell membrane lipids on the transmembrane domain of the mechanosensitive-like YnaI channel, …

Racial And Ethnic Data Reported For Peanut Allergy Epidemiology Do Little To Advance Its Cause, Treatment, Or Prevention, Nigel Mark Thomas

Publications and Research

No abstract provided.

Clock Time Of First Eating Episode And Prospective Risk Of All-Cause Mortality In Us Adults, Ashima K. Kant, Barry I. Graubard

Publications and Research

Background: There is increasing recognition that morning or evening preference is associated with time of eating, metabolic health, and morbidity. However, few studies have examined the association of time of eating with mortality.

Objective: To examine the association of time of first recalled ingestive episode with prospective risk of all-cause mortality.

Design: We used mortality-linked data from the National Health and Nutrition Examination Surveys (NHANES) conducted in 1988-1994 and 1999-2014 (n=34609; age >40 y). Quartiles (Q1-Q4) of clock time of first eating episode self-reported in the baseline 24-h dietary recall was the exposure. Follow-up time from date of NHANES examination …

Regulatory Non-Coding Rnas Modulate Transcriptional Activation During B Cell Development, Mary Attaway, Tzippora Chwat-Edelstein, Bao Q. Vuong

Publications and Research

B cells play a significant role in the adaptive immune response by secreting immunoglobulins that can recognize and neutralize foreign antigens. They develop from hematopoietic stem cells, which also give rise to other types of blood cells, such as monocytes, neutrophils, and T cells, wherein specific transcriptional programs define the commitment and subsequent development of these different cell lineages. A number of transcription factors, such as PU.1, E2A, Pax5, and FOXO1, drive B cell development. Mounting evidence demonstrates that non-coding RNAs, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), modulate the expression of these transcription factors directly by binding …

Tectonic And Geotechnical Review Of Bengal Basin For Seismic Risk Assessment In Bangladesh, Mir Fazlul Karim, Jnana Ranjan Kayal, Daya Shanker, Nazrul I. Khandaker, Arif M. Sikder, M Zillur Rahman, Muhammad Qumrul Hassan

Publications and Research

Four major geotectonic provinces of the basin are recognized: 1) the continental slope to the west of the Hinge Zone, 2) the stable shelf, 3) the deep central trough (Sylhet-Hatiya) and 4) the Chittagong-Tripura fold belt to the east. The ~300 km long Dauki Fault demarcates the elevated Shillong Plateau, part of the Indian Shield to the north and the deep basin to the south. The basin experienced three strong to major intraplate earthquakes: a) 1885 Bengal earthquake (rev. Mw 6.8) close to the Hinge Zone, b) 1918 Srimangal earthquake (rev. Mw 7.1) on the Sylhet (trough) fault and c) …

Expression, Purification And Refolding Of A Human Na V 1.7 Voltage Sensing Domain With Native-Like Toxin Binding Properties, Ryan V. Schroder, Leah S. Cohen, Ping Wang, Joekeem D. Arizala, Sébastien F. Poget

Publications and Research

The voltage-gated sodium channel NaV1.7 is an important target for drug development due to its role in pain perception. Recombinant expression of full-length channels and their use for biophysical characterization of interactions with potential drug candidates is challenging due to the protein size and complexity. To overcome this issue, we developed a protocol for the recombinant expression in E. coli and refolding into lipids of the isolated voltage sensing domain (VSD) of repeat II of NaV1.7, obtaining yields of about 2 mg of refolded VSD from 1 L bacterial cell culture. This VSD is known to be involved in the …

A Systematic Review Of Diet Quality Index And Obesity Among Chinese Adults, Isma'il Kadam, Sudeep Neupane, Jingkai Wei, Lee Ann Fullington, Tricia Li, Ruopeng An, Li Zhao, Amy Ellithorpe, Xinyin Jiang, Liang Wang

Publications and Research

Diet quality scores are designed mainly based on Western-style dietary patterns. They were demonstrated to be good indicators of obesity in developed but not developing countries. Several diet quality scores were developed based on the Chinese dietary guidelines, yet no systematic review exists regarding how they were related to obesity. We searched research articles published between 2000 and 2021 in PubMed, CINAHL, and Scopus databases. Both cross-sectional and prospective studies that examined the relationship between a diet quality score and weight, body mass index, obesity, or waist circumference conducted in a Chinese population were selected. From the 602 articles searched, …

Ecological Selectivity And The Evolution Of Mammalian Substrate Preference Across The K–Pg Boundary, Jonathan J. Hughes, Jacob S. Berv, Stephen G. B. Chester, Eric J. Sargis, Daniel J. Field

Publications and Research

The Cretaceous–Paleogene (K–Pg) mass extinction 66 million years ago was characterized by a worldwide ecological catastrophe and rapid species turnover. Large-scale devastation of forested environments resulting from the Chicxulub asteroid impact likely influenced the evolutionary trajectories of multiple clades in terrestrial environments, and it has been hypothesized to have biased survivorship in favour of nonarboreal lineages across the K–Pg boundary. Here, we evaluate patterns of substrate preferences across the K–Pg boundary among crown group mammals, a group that underwent rapid diversification following the mass extinction. Using Bayesian, likelihood, and parsimony reconstructions, we identify patterns of mammalian ecological selectivity that are …

“School Hard” And Traditional Education In The Buffyverse Alia R. Tyner-Mullings, Alia R. Tyner

Publications and Research

Buffy the Vampire Slayer was once heralded as a beacon of feminist power on television in a sea of one-dimensional wives, girlfriends, and supporting characters. Joss Whedon, its creator, has been hailed as a feminist icon and a lifesaver of kids and adults struggling with being different (Cochran). “Geeks love Joss Whedon” according to a 2012 Wired article: “His characters are smart and self-aware. He’s steeped in pop culture and has a clever way with twists and turns of science fiction tropes […] plus, he writes female character who kick ass, which makes him so rare as to be sui …

"Writing Unspeakable Things: Speechlessness, Abjection, And The Ethics/Aesthetics Of (Not) Representing Sexual Violence In Three Korean American "Comfort Women' Novels", M Laura Barberan Reinares

Publications and Research

This article analyzes “comfort women” novels by the Korean American authors Therese Park, Nora Okja Keller, and Chang-rae Lee in terms of the ethics and aesthetics of representing sexual violence through language. I specifically look at these authors’ stylistic strategies to describe rape in writing and how they (more or less successfully) avoid voyeuristic portrayals of the “comfort women” by controlling the narrative point of view and relying on traumatic “speechlessness” and abjection. Building on relevant notions from the fields of photography, cinematography, philosophy, and psychoanalysis, this analysis suggests that in-progress representations of rape in language become a futile project …

Globe Mosquito Habitat Mapper Citizen Science Data 2017–2020, Russanne Low, Rebecca Boger, Peder Nelson, Matteo Kimura

Publications and Research

The GLOBE Program's GLOBE Observer application is a free citizen science mobile data collection and visualization tool compatible with iOS and Android operating systems. Citizen scientists armed with the app can report the mosquito larval habitats they identify using the GLOBE Mosquito Habitat Mapper tool. This data can complement the climate, weather, and land cover data obtained from satellite measurements by scientists who develop risk models for mosquito-borne diseases. Public participation in mosquito surveillance research provides the opportunity to obtain the volume, velocity and variety of data needed to fight the threat of vector-borne diseases, especially in under-resourced communities with …

Deism Defined And Defended, Carlo Alvaro

Publications and Research

No abstract provided.

A Conserved Structural Role For The Walker-A Lysine In P-Loop Containing Kinases, Fatlum Hajredini, Ranajeet Ghose

Publications and Research

Bacterial tyrosine kinases (BY-kinases) and shikimate kinases (SKs) comprise two structurally divergent P-loop containing enzyme families that share similar catalytic site geometries, most notably with respect to their Walker-A, Walker-B, and DxD motifs. We had previously demonstrated that in BY-kinases, a specific interaction between the Walker-A and Walker-B motifs, driven by the conserved “catalytic” lysine housed on the former, leads to a conformation that is unable to efficiently coordinate Mg²⁺•ATP and is therefore incapable of chemistry. Here, using enhanced sampling molecular dynamics simulations, we demonstrate that structurally similar interactions between the Walker-A and Walker-B motifs, also mediated by …

Building A Feminist Commons In The Time Of Covid-19, Miriam Ticktin

Publications and Research

The global response to the COVID-19 pandemic has been structured around the idea that human connection and sociality are bad—they are dangerous. This essay suggests that, perhaps paradoxically, rather than isolating to stay healthy, people are forging new egalitarian forms of connection. I argue that COVID-19 has enhanced experiments in what I will call a “burgeoning feminist commons.” These foreground new, horizontal forms of sociality, and they build the grounds of resistance, refusing to separate the time of political organization from that of reproduction. I discuss three such experiments: masked mobs, friendly fridges, and pandemic pods. Each form of connection …

Urgent Need For Integrated Pandemic Policies On Pathogen Spillover, Pallavi A. Kache, Sonila Cook, Nigel Sizer, Lee Hannah, Neil M. Vora

Publications and Research

No abstract provided.

Reports And Commands: Deciphering A Health Exhibition Using The Speaking Mnemonic, David H. Lee

Publications and Research

The Amazing You exhibition at the Tampa Museum of Science and Industry had over 400 different multimedia health exhibits. Visitors walked through life stages, from conception through death, the exhibits at first showcasing developmental milestones, then diseases and chronic conditions associated with ageing. Museum executives described the exhibition as a public health intervention that stressed disease prevention, screening and behaviour change. This piece considers the question: What makes an exhibition be a health intervention? To describe complexities of the communication environment I use a mnemonic device called SPEAKING, an acronym for ‘Scene/Setting, Participants, Ends, Act Sequence, Key, Instrumentalites, Norms and …

Factors Influencing The Relationship Between Work-Related Stress And Posttraumatic Stress Disorder Among Working Black Adults In The United States, Paul C. Archibald

Publications and Research

Introduction: Work-related stress (WRS) and posttraumatic disorder (PTSD) is higher among Black adults relative to their White counterparts. Trauma exposure is not the only connection to increased risk for PTSD as WRS is highly associated with risk for PTSD. However, the factors that link WRS and PTSD among working Black adults is not well understood. Materials and Methods: Cross-sectional data from the National Survey of American Life was used to examine the relationship between WRS and PTSD among 2,139 working Black adults and to determine whether there are influencing factors. Results: Logistic regression analyses revealed that working …

Conjunctional Concepts: The Conceptual Teaching Technique In Biology Classes, Igor V. Zaitsev

Publications and Research

Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categories). Revealing and using conceptual conjunctions in the science classroom means teaching conceptually. To make conceptual teaching more accessible to biology instructors, a three-concept model was described to be used during a lecture. This model has allowed the author to develop a relatively simple method that starts with an active concept or concepts, then introduces conjunctional and supporting concepts to relate the content to other concepts and relevant real-world applications. This method is intended to help instructors in preparing to teach biological concepts conceptually, in order to …

Intergenerational Differences In Dietary Acculturation Among Ghanaian Immigrants Living In New York City: A Qualitative Study, Margrethe F. Horlyck-Romanovsky, Terry T.-K Huang, Ramatu Ahmed, Sandra E. Echeverria, Katarzyna Wyka, May May Leung, Anne E. Sumner, Melissa Fuster

Publications and Research

Dietary acculturation may explain the increasing risk of diet-related diseases among African immigrants in the United States (US). We interviewed twenty-five Ghanaian immigrants (Youth n 13, Age (Mean ± SD) 20 y±5⋅4, Parents (n 6) and Grandparents (n 6) age 58⋅7± 9⋅7) living in New York City (NYC) to (a) understand how cultural practices and the acculturation experience influence dietary patterns of Ghanaian immigrants and (b) identify intergenerational differences in dietary acculturation among Ghanaian youth, parents and grandparents. Dietary acculturation began in Ghana, continued in NYC and was perceived as a positive process. At the interpersonal level, parents encouraged youth …

Hostile, Quick-Tempered, And Exposed To Dangerous Environments: Exploring The Link Between Temperament And Street Code Adherence, Kevin T. Wolff, Sharron Spriggs, Jonathan Intravia, Michael T. Baglivio, Matt Delisi

Publications and Research

Although Elijah Anderson’s (1999) code of the street thesis has received a great deal of scholarly attention, fewer studies have examined the characteristics associated with its adoption. Existing evidence is supportive of Anderson’s initial observations, however, less is known about the association between personality and emotional characteristics and adopting street code norms. The current study assesses the role of Delisi and Vaughn’s difficult temperament index in the adoption of the street code among a sample of juvenile justice-involved youth. Results indicated youth with more difficult temperaments, characterized by lower levels of effortful control and higher levels of negative emotionality, were …

Crowd Salience Heightens Tolerance To Healthy Facial Features, Mitch Brown, Ryan E. Tracy, Steven G. Young, Donald F. Sacco

Publications and Research

Objective: Recent findings suggest crowd salience heightens pathogen-avoidant motives, serving to reduce individuals’ infection risk through interpersonal contact. Such experiences may similarly facilitate the identification, and avoidance, of diseased conspecifics. The current experiment sought to replicate and extend previous crowding research.

Methods: In this experiment, we primed participants at two universities with either a crowding or control experience before having them evaluate faces manipulated to appear healthy or diseased by indicating the degree to which they would want to interact with them.

Results: Crowding-primed participants reported a more heightened preferences for healthy faces than control-primed participants. Additionally, crowd salience reduced …