Digital Commons Network^™

Motivational Interviewing Targeting Risk Behaviors For Youth Living With Hiv In Thailand, Chokechai Rongkavilit, Sylvie Naar-King, Bo Wang, Apirudee Panthong, Torsak Bunupuradah, Jeffrey T. Parsons, Supalak Phonphithak, Juline A. Koken, Pichai Saengcharnchai, Praphan Phanuphak

Publications and Research

Healthy Choices, a four-session motivational interviewing-based intervention, reduces risk behaviors among US youth living with HIV (YLWH). We randomized 110 Thai YLWH (16–25 years) to receive either Healthy Choices or time-matched health education (Control) over 12 weeks. Risk behaviors were assessed at baseline, 1, and 6 months post-session. The pilot study was not powered for between-group differences; there were no statistical differences in sexual risks, alcohol use, and antiretroviral adherence between the two groups at any visit. In within-group analyses, Healthy Choices group demonstrated decreases in the proportion of HIV-negative partners (20 vs 8.2 %, P = 0.03) and HIV …

Serum Amyloid P Inhibits Granulocyte Adhesion, Anu S. Maharjan, David Roife, Derrick Brazill, Richard H. Gomer

Publications and Research

Background The extravasation of granulocytes (such as neutrophils) at a site of inflammation is a key aspect of the innate immune system. Signals from the site of inflammation upregulate granulocyte adhesion to the endothelium to initiate extravasation, and also enhance granulocyte adhesion to extracellular matrix proteins to facilitate granulocyte movement through the inflamed tissue. During the resolution of inflammation, other signals inhibit granulocyte adhesion to slow and ultimately stop granulocyte influx into the tissue. In a variety of inflammatory diseases such as acute respiratory distress syndrome, an excess infiltration of granulocytes into a tissue causes undesired collateral damage, and being …

Allometric Scaling And Resource Limitations Model Of Tree Heights: Part 1. Model Optimization And Testing Over Continental Usa, Yuli Shi, Sungho Choi, Xiliang Ni, Sangram Ganguly, Gong Zhang, Hieu V. Duong, Michael A. Lefsky, Marc Simard, Sassan S. Saatchi, Shihyan Lee, Wenge Ni-Meister, Shilong Piao, Chunxiang Cao, Ramakrishna R. Nemani, Ranga B. Myneni

Publications and Research

A methodology to generate spatially continuous fields of tree heights with an optimized Allometric Scaling and Resource Limitations (ASRL) model is reported in this first of a multi-part series of articles. Model optimization is performed with the Geoscience Laser Altimeter System (GLAS) waveform data. This methodology is demonstrated by mapping tree heights over forested lands in the continental USA (CONUS) at 1 km spatial resolution. The study area is divided into 841 eco-climatic zones based on three forest types, annual total precipitation classes (30 mm intervals) and annual average temperature classes (2 °C intervals). Three model parameters (area of single …

Functional Neural Correlates Of Attentional Deficits In Amnestic Mild Cognitive Impairment, Nicholas T. Van Dam, Mary Sano, Effie M. Mitsis, Hillel T. Grossman, Xiaosi Gu, Yunsoo Park, Patrick R. Hof, Jin Fan

Publications and Research

Although amnestic mild cognitive impairment (aMCI; often considered a prodromal phase of Alzheimer’s disease, AD) is most recognized by its implications for decline in memory function, research suggests that deficits in attention are present early in aMCI and may be predictive of progression to AD. The present study used functional magnetic resonance imaging to examine differences in the brain during the attention network test between 8 individuals with aMCI and 8 neurologically healthy, demographically matched controls. While there were no significant behavioral differences between groups for the alerting and orienting functions, patients with aMCI showed more activity in neural regions …

Effects Of Carpal Tunnel Syndrome On Dexterous Manipulation Are Grip Type-Dependent, Wei Zhang, Jamie A. Johnston, Mark A. Ross, Kyle Sanniec, Elizabeth A. Gleason, Amylou C. Dueck, Marco Santello

Publications and Research

Carpal tunnel syndrome (CTS) impairs sensation of a subset of digits. Although the effects of CTS on manipulation performed with CTS-affected digits have been studied using precision grip tasks, the extent to which CTS affects multi-digit force coordination has only recently been studied. Whole-hand manipulation studies have shown that CTS patients retain the ability to modulate multi-digit forces to object mass, mass distribution, and texture. However, CTS results in sensorimotor deficits relative to healthy controls, including significantly larger grip force and lower ability to balance the torques generated by the digits. Here we investigated the effects of CTS on multi-digit …

A Guide To Enterotypes Across The Human Body: Meta- Analysis Of Microbial Community Structures In Human Microbiome Datasets, Omry Koren, Dan Knights, Antonio Gonzalez, Levi Waldron, Nicola Segata, Rob Knight, Curtis Huttenhower, Ruth E. Ley

Publications and Research

Recent analyses of human-associated bacterial diversity have categorized individuals into ‘enterotypes’ or clusters based on the abundances of key bacterial genera in the gut microbiota. There is a lack of consensus, however, on the analytical basis for enterotypes and on the interpretation of these results. We tested how the following factors influenced the detection of enterotypes: clustering methodology, distance metrics, OTU-picking approaches, sequencing depth, data type (whole genome shotgun (WGS) vs.16S rRNA gene sequence data), and 16S rRNA region. We included 16S rRNA gene sequences from the Human Microbiome Project (HMP) and from 16 additional studies and WGS sequences from …

Allometric Scaling And Resource Limitations Model Of Tree Heights: Part 2. Site Based Testing Of The Model, Sungho Choi, Xiliang Ni, Yuli Shi, Sangram Ganguly, Gong Zhang, Hieu V. Duong, Michael A. Lefsky, Marc Simard, Sassan S. Saatchi, Shihyan Lee, Wenge Ni-Meister, Shilong Piao, Chunxiang Cao, Ramakrishna R. Nemani, Ranga B. Myneni

Publications and Research

The ultimate goal of this multi-article series is to develop a methodology to generate continuous fields of tree height and biomass. The first paper demonstrated the need for Allometric Scaling and Resource Limitation (ASRL) model optimization and its ability to generate spatially continuous fields of tree heights over the continental USA at coarse (1 km) spatial resolution. The objective of this second paper is to provide an assessment of that approach at site scale, specifically at 12 FLUXNET sites where more accurate data are available. Estimates of tree heights from the Geoscience Laser Altimeter System (GLAS) waveform data are used …

The Prion Dilemma Confounding Science Educators, Igor V. Zaitsev, Ling Chen, Susie Boydston-White, Manita Pavel, Svetlana N. Shugaeva, Alla G. Petrova, Oksana V. Williams

Publications and Research

In this paper, the issue of the prion hypothesis, a simmering controversy within the scientific community, is addressed. We inquire into the appropriateness of the use of certain augmentations and rhetoric approaches used during scientific debates, as well as the aptness of unequivocal statements in textbooks that indicate “abnormal prions” as a primary cause of Transmissible Spongiform Encephalopathies.

Tolerance Of Roadside And Old Field Populations Of Common Teasel (Dipsacus Fullonum Subsp. Sylvestris) To Salt And Low Osmotic Potentials During Germination, Laura L. Beaton, Susan A. Dudley

Publications and Research

Maternal families from roadside populations of Dipsacus fullonum subsp. sylvestris displayed greater tolerance of both high salinity and drought during germination than families from old field populations. However, the tolerances were not correlated, indicating that they are separate traits.

Changes In Natural Killer Cell Activation And Function During Primary Hiv-1 Infection, Vivek Naranbhai, Marcus Altfeld, Salim S. Abdool Karim, Thumbi Ndung’U, Quarraisha Abdool Karim, William H. Carr

Publications and Research

Background: Recent reports suggest that Natural Killer (NK) cells may modulate pathogenesis of primary HIV-1 infection. However, HIV dysregulates NK-cell responses. We dissected this bi-directional relationship to understand how HIV impacts NK-cell responses during primary HIV-1 infection.

Methodology/Principal Findings: Paired samples from 41 high-risk, initially HIV-uninfected CAPRISA004 participants were analysed prior to HIV acquisition, and during viraemic primary HIV-1 infection. At the time of sampling post-infection five women were seronegative, 11 women were serodiscordant, and 25 women were seropositive by HIV-1 rapid immunoassay. Flow cytometry was used to measure NK and T-cell activation, NK-cell receptor expression, cytotoxic and cytokine-secretory functions, …

High Levels Of Adherence And Viral Suppression In A Nationally Representative Sample Of Hiv-Infected Adults On Antiretroviral Therapy For 6, 12 And 18 Months In Rwanda, Batya Elul, Paulin Basinga, Harriet Nuwagaba-Biribonwoha, Suzue Saito, Deborah Horowitz, Denis Nash, Jules Mugabo, Veronicah Mugisha, Etienne Rugigana, Richard Nkunda, Anita Asiimwe

Publications and Research

Background Generalizable data are needed on the magnitude and determinants of adherence and virological suppression among patients on antiretroviral therapy (ART) in Africa. Methods We conducted a cross-sectional survey with chart abstraction, patient interviews and site assessments in a nationally representative sample of adults on ART for 6, 12 and 18 months at 20 sites in Rwanda. Adherence was assessed using 3- and 30-day patient recall. A systematically selected sub-sample had viral load (VL) measurements. Multivariable logistic regression examined predictors of non-perfect (<100%) 30-day adherence and detectable VL (>40 copies/ml). Results Overall, 1,417 adults were interviewed and 837 had VL measures. Ninety-four percent and 78% …

Differential Transcriptional Regulation Of Meis1 By Gfi1b And Its Co-Factors Lsd1 And Corest, Asif H. Chowdhury, Johnny R. Ramroop, Ghanshyam Upadhyay, Ananya Sengupta, Anna Andrzejczyk, Shireen Saleque

Publications and Research

Gfi1b (growth factor independence 1b) is a zinc finger transcription factor essential for development of the erythroid and megakaryocytic lineages. To elucidate the mechanism underlying Gfi1b function, potential downstream transcriptional targets were identified by chromatin immunoprecipitation and expression profiling approaches. The combination of these approaches revealed the oncogene meis1, which encodes a homeobox protein, as a direct and prominent target of Gfi1b. Examination of the meis1 promoter sequence revealed multiple Gfi1/1b consensus binding motifs. Distinct regions of the promoter were occupied by Gfi1b and its cofactors LSD1 and CoREST/Rcor1, in erythroid cells but not in the closely related megakaryocyte lineage. …

Autophagy Plays An Essential Role In Mediating Regression Of Hypertrophy During Unloading Of The Heart, Nirmala Hariharan, Yoshiyuki Ikeda, Chull Hong, Ralph R. Alcendor, Soichiro Usui, Shumin Gao, Yasuhiro Maejima, Junichi Sadoshima

Publications and Research

Autophagy is a bulk degradation mechanism for cytosolic proteins and organelles. The heart undergoes hypertrophy in response to mechanical load but hypertrophy can regress upon unloading. We hypothesize that autophagy plays an important role in mediating regression of cardiac hypertrophy during unloading. Mice were subjected to transverse aortic constriction (TAC) for 1 week, after which the constriction was removed (DeTAC). Regression of cardiac hypertrophy was observed after DeTAC, as indicated by reduction of LVW/BW and cardiomyocyte cross-sectional area. Indicators of autophagy, including LC3-II expression, p62 degradation and GFP-LC3 dots/cell, were significantly increased after DeTAC, suggesting that autophagy is induced. Stimulation …

The Telomere Capping Complex Cst Has An Unusual Stoichiometry, Makes Multipartite Interaction With G-Tails, And Unfolds Higher-Order G-Tail Structures, Neal F. Lue, Ruobo Zhou, Lidia Chico, Ninghui Mao, Olga Steinberg-Neifach, Taekjip Ha

Publications and Research

The telomere-ending binding protein complex CST (Cdc13-Stn1-Ten1) mediates critical functions in both telomere protection and replication. We devised a co-expression and affinity purification strategy for isolating large quantities of the complete Candida glabrata CST complex. The complex was found to exhibit a 2∶4∶2 or 2∶6∶2 stoichiometry as judged by the ratio of the subunits and the native size of the complex. Stn1, but not Ten1 alone, can directly and stably interact with Cdc13. In gel mobility shift assays, both Cdc13 and CST manifested high-affinity and sequence-specific binding to the cognate telomeric repeats. Single molecule FRET-based analysis indicates that Cdc13 and …

Revisiting The Evolution Of Mouse Line-1 In The Genomic Era, Akash Sookdeo, Crystal M. Hepp, Marcella A. Mcclure, Stéphane Boissinot

Publications and Research

Background LINE-1 (L1) is the dominant category of transposable elements in placental mammals. L1 has significantly affected the size and structure of all mammalian genomes and understanding the nature of the interactions between L1 and its mammalian host remains a question of crucial importance in comparative genomics. For this reason, much attention has been dedicated to the evolution of L1. Among the most studied elements is the mouse L1 which has been the subject of a number of studies in the 1980s and 1990s. These seminal studies, performed in the pre-genomic era when only a limited number of L1 sequences …

From Toxic Tours To Growing The Grassroots: Tensions In Critical Pedagogy And Community Development, Celina Su, Isabelle Jagninski

Publications and Research

Structural inequalities in American public education are inextricably tied to deep-seated patterns of racial and economic segregation. Children in poor neighborhoods are less likely to have the household resources, neighborhood institutions, or school amenities necessary for a good, challenging education. In response, a growing number of organizations have launched initiatives to simultaneously revitalize neighborhoods and improve public education, emphasizing youth participation as an essential component in their efforts. We draw upon ethnographic data from two such organizations to examine their practice of place-based critical pedagogy in community development. We focus on how they engage marginalized, “hard-to-reach” youth via (1) experiential …

Is Anyone Regulating?: The Curious State Of Gmo Governance In The United States, Rebecca Bratspies

Publications and Research

No abstract provided.

Health Communication Closely Tied To Culture, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Panama Trip Will Explore Biodiversity Hotspot, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

O’Brien Studies The Origins Of The “Moron.”, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Students Learn More Than Spanish In Spain, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Surviving The City: Resistance And Plant Life In Woolf’S Jacob’S Room And Barnes’ Nightwood, Ria Banerjee

Publications and Research

In Jacob’s Room (1922) and Nightwood (1936), Virginia Woolf and Djuna Barnes use plant life to express a profound ambivalence about the masculine-inflected ordering functions of art and morality. They show that these processes codify lived experience and distance it from the feminine and sexual. To counter this turn towards the urban inauthentic, both novels depict non-urban spaces to upend conventional notions of usefulness. They fixate on evanescent flowers, wild forests, and untillable fields as sites of resistance whose fragility and remoteness are strengths. In Jacob’s Room, I argue that the eponymous protagonist is destroyed by his conventional education …

Google Vs. The Library: Student Preferences And Perceptions When Doing Research Using Google And A Federated Search Tool, Helen Georgas

Publications and Research

Federated searching was once touted as the library world’s answer to Google, but ten years since federated searching technology’s inception, how does it actually compare? This study focuses on undergraduate student preferences and perceptions when doing research using both Google and a federated search tool. Students were asked about their preferences using each search tool and the perceived relevance of the sources they found using each search tool. Students were also asked to self-assess their online searching skills. The findings show that students believe they possess strong searching skills, are able to find relevant sources using both search tools, but …

Biomarkers For Brain Disorders Electrochemically Detected By Broderick Probe Microelectrodes/Biosensors, Waqas Saleem, Patricia A. Broderick

Publications and Research

Here, we present results from two independent studies carried out using Neuromolecular Imaging (NMI) with miniature BRODERICK PROBE® biosensors. In the first study, we imaged neurotransmitters and neurochemicals in human epilepsy patients intraoperatively during early and late neurodegeneration. In the second study, we imaged neurotransmitters and neurochemicals in an experimental murine model using animals with and without neurodegeneration caused by Parkinson’s disease (PD). We compared our results derived from animals with lesioned group (PD) with non-lesioned group (non-PD), using the same in vivo NMI paradigm. NMI biotechnology enabled neurotransmitters, neuropeptides and neurochemical imaging of dopamine (DA), serotonin (5-HT), homovanillic acid …

Corticospinal Reorganization After Locomotor Training In A Person With Motor Incomplete Paraplegia, Nupur Hajela, Chaithanya K. Mummidisetty, Andrew C. Smith, Maria Knikou

Publications and Research

Activity-dependent plasticity as a result of reorganization of neural circuits is a fundamental characteristic of the central nervous system that occurs simultaneously in multiple sites. In this study, we established the effects of subthreshold transcranial magnetic stimulation (TMS) over the primary motor cortex region on the tibialis anterior (TA) long-latency flexion reflex. Neurophysiological tests were conducted before and after robotic gait training in one person with a motor incomplete spinal cord injury (SCI) while at rest and during robotic-assisted stepping. The TA flexion reflex was evoked following nonnociceptive sural nerve stimulation and was conditioned by TMS at 0.9 TA motor …

The Role Of Big Data In The Social Sciences, Steven Ovadia

Publications and Research

This article explores the role of big data in the social sciences and reviews some online sources of large data sets.

Isabel Archer's "Delicious Pain": Charting Lacanian Desire In The Portrait Of A Lady, Phyllis E. Vanslyck

Publications and Research

This essay offers a reading of Henry James's Portrait of a Lady that examines Isabel Archer's choices through a Lacanian lens. This reading traces Isabel's consistent turning away from, even against, the very postulates she claims to live by. Isabel’s discovery of love through the ideal image of herself she finds mirrored in Gilbert Osmond’s gaze leads to a reversal of her most noble impulses. Her choice of a suitor also points to something that would seem the opposite of desire, but which is, in fact, its foundation. In choosing Gilbert Osmond, Isabel seeks to experience, however unconsciously, what Jacques …

‘What’S In The Niddk Cdr?’—Public Query Tools For The Niddk Central Data Repository, Huaqin Pan, Mary-Anne Ardini, Vesselina Bakalov, Michael Delatte, Paul Eggers, Laxminarayana Ganapathi, Craig R. Hollingsworth, Joshua Levy, Sheping Li, Joseph Pratt, Norma Pugh, Ying Qin, Rebekah Rasooly, Helen Ray, Jean E. Richardson, Amanda Flynn Riley, Susan M. Rogers, Sylvia Tan, Charles F. Turner, Stacie White, Philip C. Cooley

Publications and Research

The National Institute of Diabetes and Digestive Disease (NIDDK) Central Data Repository (CDR) is a web-enabled resource available to researchers and the general public. The CDR warehouses clinical data and study documentation from NIDDK funded research, including such landmark studies as The Diabetes Control and Complications Trial (DCCT, 1983–93) and the Epidemiology of Diabetes Interventions and Complications (EDIC, 1994–present) follow-up study which has been ongoing for more than 20 years. The CDR also houses data from over 7 million biospecimens representing 2 million subjects. To help users explore the vast amount of data stored in the NIDDK CDR, we developed …

What To Eat Now? Shifts In Polar Bear Diet During The Ice-Free Season In Western Hudson Bay, Linda J. Gormezano, Robert F. Rockwell

Publications and Research

Under current climate trends, spring ice breakup in Hudson Bay is advancing rapidly, leaving polar bears (Ursus maritimus) less time to hunt seals during the spring when they accumulate the majority of their annual fat reserves. For this reason, foods that polar bears consume during the ice-free season may become increasingly important in alleviating nutritional stress from lost seal hunting opportunities. Defining how the terrestrial diet might have changed since the onset of rapid climate change is an important step in understanding how polar bears may be reacting to climate change. We characterized the current terrestrial diet of polar bears …

Creating Room For A Singularity Of Our Own: Reading Sue Lange's "We, Robots", Marleen S. Barr

Publications and Research

The accessibility of Lange’s text might mitigate against recognizing its importance. Lange’s simple sentence structure and direct communicative mode convey a presently overlooked logical moral assertion: the impending Singularity is not a male-dominated patriarchal domain. The Singularity, in other words, should not be construed in a manner which excludes women and feminism. This assertion is patently obvious. But, nonetheless, it is often ignored. Before I read Lange’s novella as a description of the Singularity which feminists can embrace, I include the following background information: 1) a discussion about why the discourse relating to the Singularity needs to be expanded and …