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Direct Control Of Visual Perception With Phase-Specific Modulation Of Posterior Parietal Cortex, Andrew Jaegle, Tony Ro

Publications and Research

We examined the causal relationship between the phase of alpha oscillations (9–12 Hz) and conscious visual perception using rhythmic TMS (rTMS) while simultaneously recording EEG activity. rTMS of posterior parietal cortex at an alpha frequency (10 Hz), but not occipital or sham rTMS, both entrained the phase of subsequent alpha oscillatory activity and produced a phase-dependent change on subsequent visual perception, with lower discrimination accuracy for targets presented at one phase of the alpha oscillatory waveform than for targets presented at the opposite phase. By extrinsically manipulating the phase of alpha before stimulus presentation, we provide direct evidence that the …

New Insights Into The Promoterless Transcription Of Dna Coligo Templates By Rna Polymerase Iii, Lodoe Lama, Christine I. Seidl, Kevin Ryan

Publications and Research

Chemically synthesized DNA can carry small RNA sequence information but converting that information into small RNA is generally thought to require large double-stranded promoters in the context of plasmids, viruses and genes. We previously found evidence that circularized oligodeoxynucleotides (coligos) containing certain sequences and secondary structures can template the synthesis of small RNA by RNA polymerase III in vitro and in human cells. By using immunoprecipitated RNA polymerase III we now report corroborating evidence that this enzyme is the sole polymerase responsible for coligo transcription. The immobilized polymerase enabled experiments showing that coligo transcripts can be formed through transcription termination …

Peptide Detection Of Fungal Functional Amyloids In Infected Tissue, Melissa C. Garcia-Sherman, Nataliya Lysak, Alexandra Filonenko, Hazel Richards, Richard E. Sobonya, Stephen A. Klotz, Peter N. Lipke

Publications and Research

Many fungal cell adhesion proteins form functional amyloid patches on the surface of adhering cells. The Candida albicans Agglutinin-like sequence (Als) adhesins are exemplars for this phenomenon, and have amyloid forming sequences that are conserved between family members. The Als5p amyloid sequence mediates amyloid fibril formation and is critical for cell adhesion and biofilm formation, and is also present in the related adhesins Als1p and Als3p. We have developed a fluorescent peptide probe containing the conserved Als amyloid-forming sequence. This peptide bound specifically to yeast expressing Als5p, but not to cells lacking the adhesin. The probe bound to both yeast …

Inhibition Of Extracellular Hmgb1 Attenuates Hyperoxia-Induced Inflammatory Acute Lung Injury, Maria Entezari, Mohammad Javdan, Daniel J. Antoine, Dympna M.P. Morrow, Ravikumar A. Sitapara, Vivek Patel, Mao Wang, Lokesh Sharma, Samir Gorasiya, Michelle Zur, Wenjun Wu, Jianhua Li, Huan Yang, Charles R. Ashby, Douglas Thomas, Haichao Wang, Lin L. Mantell

Publications and Research

Prolonged exposure to hyperoxia results in acute lung injury (ALI), accompanied by a significant elevation in the levels of proinflammatory cytokines and leukocyte infiltration in the lungs. However, the mechanisms underlying hyperoxia-induced proinflammatory ALI remain to be elucidated. In this study, we investigated the role of the proinflammatory cytokine high mobility group box protein 1 (HMGB1) in hyperoxic inflammatory lung injury, using an adult mouse model. The exposure of C57BL/6 mice to ≥99% O2 (hyperoxia) significantly increased the accumulation of HMGB1 in the bronchoalveolar lavage fluids (BALF) prior to the onset of severe inflammatory lung injury. In the airways of …

The Adaptive Remodeling Of Endothelial Glycocalyx In Response To Fluid Shear Stress, Ye Zeng, John M. Tarbell

Publications and Research

The endothelial glycocalyx is vital for mechanotransduction and endothelial barrier integrity. We previously demonstrated the early changes in glycocalyx organization during the initial 30 min of shear exposure. In the present study, we tested the hypothesis that long-term shear stress induces further remodeling of the glycocalyx resulting in a robust layer, and explored the responses of membrane rafts and the actin cytoskeleton. After exposure to shear stress for 24 h, the glycocalyx components heparan sulfate, chondroitin sulfate, glypican-1 and syndecan-1, were enhanced on the apical surface, with nearly uniform spatial distributions close to baseline levels that differed greatly from the …

Quality-Space Theory In Olfaction, Benjamin D. Young, Andreas Keller, David Rosenthal

Publications and Research

Quality-space theory (QST) explains the nature of the mental qualities distinctive of perceptual states by appeal to their role in perceiving. QST is typically described in terms of the mental qualities that pertain to color. Here we apply QST to the olfactory modalities. Olfaction is in various respects more complex than vision, and so provides a useful test case for QST. To determine whether QST can deal with the challenges olfaction presents, we show how a quality space (QS) could be constructed relying on olfactory perceptible properties and the olfactory mental qualities then defined by appeal to that QS of …

Seasonal Mesophotic Coral Bleaching Of Stylophora Pistillata In The Northern Red Sea, Orit Nir, David F. Gruber, Eli Shemesh, Eliezra Glasser, Dan Tchernov

Publications and Research

Coral bleaching occurs when environmental stress induces breakdown of the coral-algae symbiosis and the host initiates algae expulsion. Two types of coral bleaching had been thoroughly discussed in the scientific literature; the first is primarily associated with mass coral bleaching events; the second is a seasonal loss of algae and/or pigments. Here, we describe a phenomenon that has been witnessed for repeated summers in the mesophotic zone (40–63 m) in the northern Red Sea: seasonal bleaching and recovery of several hermatypic coral species. In this study, we followed the recurring bleaching process of the common coral Stylophora pistillata. Bleaching occurred …

Wave Function For Harmonically Confined Electrons In Time-Dependent Electric And Magnetostatic Fields, Hong-Ming Zhu, Jin-Wang Chen, Xiao-Yin Pan, Viraht Sahni

Publications and Research

We derive via the interaction “representation” the many-body wave function for harmonically confined electrons in the presence of a magnetostatic field and perturbed by a spatially homogeneous time-dependent electric field—the Generalized Kohn Theorem (GKT) wave function. In the absence of the harmonic confinement – the uniform electron gas – the GKT wave function reduces to the Kohn Theorem wave function. Without the magnetostatic field, the GKTwave function is the Harmonic Potential Theorem wave function. We further prove the validity of the connection between the GKT wave function derived and the system in an accelerated frame of reference. Finally, we provide …

The Covert World Of Fish Biofluorescence: A Phylogenetically Widespread And Phenotypically Variable Phenomenon, John S. Sparks, Robert C. Schelly, W. Leo Smith, Matthew P. Davis, Dan Tchernov, Vincent A. Pieribone, David F. Gruber

Publications and Research

The discovery of fluorescent proteins has revolutionized experimental biology. Whereas the majority of fluorescent proteins have been identified from cnidarians, recently several fluorescent proteins have been isolated across the animal tree of life. Here we show that biofluorescence is not only phylogenetically widespread, but is also phenotypically variable across both cartilaginous and bony fishes, highlighting its evolutionary history and the possibility for discovery of numerous novel fluorescent proteins. Fish biofluorescence is especially common and morphologically variable in cryptically patterned coral-reef lineages. We identified 16 orders, 50 families, 105 genera, and more than 180 species of biofluorescent fishes. We have also …

Milk Consumption And Cardiovascular Risk Factors In Older Chinese: The Guangzhou Biobank Cohort Study, Yangbo Sun, Chaoqiang Jiang, Kar Keung Cheng, Weisen Zhang, Gabriel M. Leung, Tai Hing Lam, Mary Schooling

Publications and Research

Background
Dairy products consumption is increasingly common globally. Most of the evidence concerning dairy products comes from observational studies in western populations which are inevitably open to confounding. To triangulate the evidence concerning dairy products, we examined the associations of whole cow’s milk consumption with cardiovascular risk factors in a non-Western setting with a different pattern of milk consumption and cardiovascular diseases from Western populations.

Methods
We used multivariable censored linear or logistic regression to examine cross-sectionally the adjusted associations of whole cow’s milk consumption (none (n = 14892), 1–3/week (n = 2689) and 3+/week (n = 2754)) with cardiovascular …

2matrix: A Utility For Indel Coding And Phylogenetic Matrix Concatenation, Nelson R. Salinas, Damon P. Little

Publications and Research

Premise of the study: Phylogenetic analysis of DNA and amino acid sequences requires the creation of files formatted specifically for each analysis package. Programs currently available cannot simultaneously code inferred insertion/deletion (indel) events in sequence alignments and concatenate data sets.

Methods and Results: A novel Perl script, 2matrix, was created to concatenate matrices of non-molecular characters and/or aligned sequences and to code indels. 2matrix outputs a variety of formats compatible with popular phylogenetic programs.

Conclusions: 2matrix efficiently codes indels and concatenates matrices of sequences and non-molecular data. It is available for free download under a GPL (General Public License) open …

Disciplinarity And Trandisciplinarity In The Study Of Knowledge, Jay H. Bernstein

Publications and Research

Scholarly inquiry about the nature and significance of knowledge has been shaped by disciplinary traditions and priorities that define “knowledge” differently and result in disconnected literatures. In the mid to late twentieth century, library science educator Jesse Shera sought to bridge the conceptual gap between epistemological and sociological approaches to knowledge in proposing a new discipline he called social epistemology. Around the same time, long-term projects by the economist Fritz Machlup and the physical chemist turned philosopher of science Michael Polanyi did not merely combine existing disciplinary approaches but transcended conventional frameworks for conceptualizing knowledge. These scholars can be viewed …

Abortion Exceptionalism And Undue Burden Preemption, Caitlin Borgmann

Publications and Research

No abstract provided.

Sunshine For Sale: Environmental Contractors And The Freedom Of Information Act, Sarah Lamdan

Publications and Research

No abstract provided.

A Course-Based Research Experience: How Benefits Change With Increased Investment In Instructional Time, Christopher D. Shaffer, Consuelo J. Alvarez, April E. Bednarski, David Dunbar, Anya L. Goodman, Catherine Reinke, Anne G. Rosenwald, Michael J. Wolyniak, Cheryl Bailey, Daron Barnard, Christopher Bazinet, Dale L. Beach, James E. J. Bedard, Satish Bhalla, John Braverman, Martin Burg, Vidya Chandrasekaran, Hui-Min Chung, Kari Clase, Randall J. Dejong, Shubha Govind, Gerard Mcneil, Karim Sharif

Publications and Research

While course-based research in genomics can generate both knowledge gains and a greater appreciation for how science is done, a significant investment of course time is required to enable students to show gains commensurate to a summer research experience. Nonetheless, this is a very cost-effective way to reach larger numbers of students.

Social Work A Broad, Often Invisible Profession, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Is Burnout Solely Job-Related? A Critical Comment, Bianchi Renzo, Didier Truchot, Eric Laurent, Romain Brisson, Irvin Sam Schonfeld

Publications and Research

Within the field‐dominating, multidimensional theory of burnout, burnout is viewed as a work‐specific condition. As a consequence, the burnout syndrome cannot be investigated outside of the occupational domain. In the present paper, this restrictive view of burnout's scope is criticized and a rationale to decide between a work‐specific and a generic approach to burnout is presented. First, the idea that a multidimensional conception of burnout implies a work‐restricted scope is deconstructed. Second, it is shown that the burnout phenomenon cannot be confined to work because chronic, unresolvable stress – the putative cause of burnout – is not limited to …

Insects And Humans Share A Complex History, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Complement Expression In The Retina Is Not Influenced By Short-Term Pressure Elevation, Konstantin Astafurov, Cecilia Q. Dong, Lampros Panagis, Gautam Kamtham, Lizhen Ren, Anna Rozenboym, Tarique D. Perera, Jeremy D. Coplan, John Danias

Publications and Research

Purpose: To determine whether short-term pressure elevation affects complement gene expression in the retina in vitro and in vivo.

Methods: Muller cell (TR-MUL5) cultures and organotypic retinal cultures from adult mice and monkeys were sub- jected to either 24-h or 72-h of pressure at 0, 15, 30, and 45 mmHg above ambient. C57BL/6 mice were subjected to microbead-induced intraocular pressure (IOP) elevation for 7 days. RNA and protein were extracted and used for analysis of expression levels of complement component genes and complement component 1, q subcomponent (C1q) and comple- ment factor H (CFH) immunoblotting. …

Obituary: Masangu Shabangi (1965-2014)., Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Geography More Than Just Maps And Capitals, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Is Burnout A Depressive Disorder? A Reexamination With Special Focus On Atypical Depression, Renzo Bianchi, Irvin Sam Schonfeld, Eric Laurent

Publications and Research

Whether burnout and depression cover the same psychopathology remains to be elucidated. To date, subtypes of depression have been overlooked in research on the burnout–depression overlap. Our aim was to estimate the prevalence of depressive disorders in workers with burnout while examining the overlap of burnout with the atypical subtype of depression. The present study included 5,575 schoolteachers (mean age = 41 years; 78% female). Burnout was assessed with the Maslach Burnout Inventory. Depression was measured with the 9-item depression scale of the Patient Health Questionnaire (PHQ-9). Atypical features of depression were examined using a dedicated module, referenced to …

Natural And Lesion-Induced Decrease In Cell Proliferation In The Medial Nucleus Of The Trapezoid Body During Hearing Development, Aminat Saliu, Shana Adise, Sandy Xian, Kamila Kudelska, Adrián Rodríguez-Contreras

Publications and Research

The functional interactions between neurons and glial cells that are important for nervous system function are presumably established during development from the activity of progenitor cells. In this study we examined proliferation of progenitor cells in the medial nucleus of the trapezoid body (MNTB) located in the rat auditory brainstem. We performed DNA synthesis labeling experiments to demonstrate changes in cell proliferation activity during postnatal stages of development. An increase in cell proliferation correlated with MNTB growth and the presence of S100β-positive astrocytes among MNTB neurons. In additional experiments we analyzed the fate of newly born cells. At perinatal ages, …

Distribution Of Intravascular And Extravascular Extracellular Volume Fractions By Total Area Under Curve For Neovascularization Assessment By Dynamic Contrast-Enhanced Magnetic Resonance Imaging, Yi Sun

Publications and Research

In this paper, we propose and investigate distribution of intravascular and extravascular extracellular volume fractions (DIEEF) as a noninvasive biomarker for neovascularization assessment by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). A generalized two-compartment exchange model (G2CXM) that uniformly includes the Patlak model, Tofts model, extended Tofts model, and recent two-compartment exchange model as special instances is first presented. Based on the total area under curve of the G2CXM a method of DIEEF estimation without knowing the artery input function is proposed. The mean square error of DIEEF estimate in the presence of noise and with incomplete DCE-MRI data is analyzed. …

A Feasibility Study Of Motivational Interviewing For Health Risk Behaviors Among Thai Youth Living With Hiv, Chokechai Rongkavilit, Sylvie Naar-King, Juline Koken, Torsak Bunupuradah, Xinguang Chen, Pichai Saengcharnchai, Apirudee Panthong, Jeffrey T. Parsons

Publications and Research

Thailand is among the few less-developed countries with successful HIV prevention among segments of the population (National AIDS Prevention and Alleviation Committee, 2010). However, the success of HIV prevention among Thai adolescents and young adults has been questionable. The highest percentage of Thai persons with AIDS remain in the age group of 25–34 years, indicating that the time of greatest HIV acquisition has occurred in adolescence and young adulthood (Thai Working Group on HIV/AIDS, 2010). Most Thai youth living with HIV (TYLH) acquire HIV through sexual contact (National AIDS Prevention and Alleviation Committee, 2010). In a study of TYLH, only …

Case Study: Sick On A South American Sugarcane Plantation, Kevin M. Bonney

Publications and Research

A case study is presented that was designed to be used in high school and college level courses to familiarize students with chagas disease, its transmission, and effects in Central and South America. Noted objectives of the lesson include evaluating disease symptoms, examining socioeconomic factors related to the epidemiology, and creating visual aids with public health information. Questions are provided for discussing the a case of chagas disease at a Brazilian sugarcane plantation.

Falls Among The Elderly: Multi-Factorial Community-Based Falls- Prevention Programs, Ray Marks

Publications and Research

No abstract provided.

Osteoarthritis And Articular Cartilage: Biomechanics And Novel Treatment Paradigms, Ray Marks

Publications and Research

Background: Osteoarthritis is a widespread highly painful disabling age-related disease with no known cure. Although novel strategies for ameliorating osteoarthritic damage abound, it is likely that none will be successful over time if the entire spectrum of the disease and the effects of joint biomechanics on joint tissues are not carefully considered.

Objectives: 1) To detail the structure of healthy articular cartilage, the key tissue affected by osteoarthritis. 2) To detail what aspects of cartilage damage best characterize osteoarthritis. 3) To consider the role of biomechanical factors in developing solutions to treat osteoarthritic joint damage. Methods: Literature sources from 1980 …

A Central Support System Can Facilitate Implementation And Sustainability Of A Classroom-Based Undergraduate Research Experience (Cure) In Genomics, David Lopatto, Charles Hauser, Christopher J. Jones, Don Paetkau, Vidya Chandrasekaran, David Dunbar, Christy Mackinnon, Joyce Stamm, Consuelo Alvarez, Daron Barnard, James E. J. Bedard, April E. Bednarski, Satish Bhalla, John M. Braverman, Martin Burg, Hui-Min Chung, Randall J. Dejong, Justin R. Diangelo, Chunguang Du, Todd T. Eckdahl, Yuying Gosser, Shubha Govind, Gerard Mcneil, Karim A. Sharif

Publications and Research

There have been numerous calls to engage students in science as science is done. A survey of 90-plus faculty members explores barriers and incentives when developing a research-based genomics course. The results indicate that a central core supporting a national experiment can help overcome local obstacles.

Preserving The Historic Garden Suburb: Case Studies From London And New York, Jeffrey A. Kroessler

Publications and Research

The garden city or garden suburb was a response to the social and environmental ills of cities at the turn of the twentieth century. Letchworth Garden City, Hampstead Garden Suburb, and Welwyn Garden City were built outside London in the early 1900s, and each remains a highly desirable place of residence today. From the start, each was tightly regulated, and remains so a century later. By protecting the appearance and enhancing property values, the strict application of historic preservation principles contribute to the long-term sustainability of each place. Similar garden suburbs were built in the borough of Queens in New …