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In Vitro Formation Of Neuroclusters In Microfluidic Devices And Cell Migration As A Function Of Stromal-Derived Growth Factor 1 Gradients, Sean Mccutcheon, Uchenna Unachukwu, Ankush Thakur, Robert Majeska, Stephen Redenti, Maribel Vazquez

Publications and Research

Central nervous system (CNS) cells cultured in vitro as neuroclusters are useful models of tissue regeneration and disease progression. However, the role of cluster formation and collective migration of these neuroclusters to external stimuli has been largely unstudied in vitro. Here, 3 distinct CNS cell types, medulloblastoma (MB), medulloblastoma-derived glial progenitor cells (MGPC), and retinal progenitor cells (RPC), were examined with respect to cluster formation and migration in response to Stromal-Derived Growth Factor (SDF-1). A microfluidic platform was used to distinguish collective migration of neuroclusters from that of individual cells in response to controlled concentration profiles of SDF-1. Cell …

The Fragility Of Ecological Pedagogy: Elementary Social Studies Standards And Possibilities Of New Materialism, Debbie Sonu, Nathan Snaza

Publications and Research

The greatest challenge facing the field of environmentalism includes ontological questions over the human subject and its desensitization from landscapes of experience. In this article the authors draw from field experiences in New York City elementary schools (such as observations of teachers, NYS Scope and Sequence Standards for Social Studies, and the Common Core State Standards) to demonstrate how curricular engagements with nature and the environment are persistently caught within humanist traditions that place agency and action as sovereign to humanness. It uses new materialist ontologies to suggest how hybrid relations among humans, nonhumans, and matter can be read by …

Social Patterning In Adiposity In Adolescence: Prospective Observations From The Chinese Birth Cohort ‘‘Children Of 1997’’, L. L. Hui, Gabriel M. Leung, C. Mary Schooling

Publications and Research

Introduction: Low early life socio-economic position is more strongly associated with adiposity among women than men. We examined whether the sex difference of social patterning in general and central adiposity exists before adulthood.

Methods: In Hong Kong’s “Children of 1997” birth cohort, we used multivariable regression to examine the association of parental education, a marker of early life socio-economic position, with body mass index (BMI) (n = 7252, 88% follow-up) and waist-height ratio (n = 5636, 68% follow- up), at 14 years.

Results: Parental education of Grade 9 or below, compared to Grade 12 or above, was associated with higher …

Pore Structure And Synergy In Antimicrobial Peptides Of The Magainin Family, Almudena Pino-Angeles, John M. Leveritt Iii, Themis Lazaridis

Publications and Research

Magainin 2 and PGLa are among the best-studied cationic antimicrobial peptides. They bind preferentially to negatively charged membranes and apparently cause their disruption by the formation of transmembrane pores, whose detailed structure is still unclear. Here we report the results of 5–9 μs all-atom molecular dynamics simulations starting from tetrameric transmembrane helical bundles of these two peptides, as well as their stoichiometric mixture, and the analog MG-H2 in DMPC or 3:1 DMPC/DMPG membranes. The simulations produce pore structures that appear converged, although some effect of the starting peptide arrangement (parallel vs. antiparallel) is still observed on this timescale. The peptides …

Characterization Of Fluorescent Proteins For Three- And Four-Color Live-Cell Imaging In S. Cerevisiae, Ryo Higuchi-Sanabria, Enrique J. Garcia, Delia Tomoiaga, Emilia L. Munteanu, Paul Feinstein, Liza A. Pon

Publications and Research

Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/ mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with …

Drilling For Common Ground: How Public Opinion Tracks Experts In The Debate Over Federal Regulation Of Shale Oil & Gas Extraction, Elizabeth Ann Glass Geltman

Publications and Research

Public interest in environmental and health impacts from shale oil and gas extraction (what the public calls “fracking”) is growing. Industry claims the public outcry against the new technology is not grounded in science. In February 2013, Resources for the Future (“RFF”) published a list of high priority “risk pathways” that experts from NGOs, academia, government, and industry all agreed were real concerns about fracking. This article used the risk matrix to evaluate whether public comments in dockets of federal agencies that proposed regulation concerning hydraulic fracturing tracked expert concern. The article found that the public tracked many of the …

Oil & Gas Drilling In National Parks, Elizabeth Ann Glass Geltman

Publications and Research

While a great deal of public attention addresses the Halliburton loophole of the Energy Policy Act of 2005 and Bureau of Land Management efforts to regulate hydraulic fracturing on public lands, less attention has been paid to the National Park Service “9B Regulations,” which provide a national regulatory framework governing the exercise of nonfederal oil and gas rights in national parks. This article begins with a review of law pertaining to oil and gas drilling in national parks. The article examines the tension in striking a balance between environmental protection, conservation of national lands and achieving energy independence, including National …

Women In Academia Facing More Prejudices, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Burnout-Depression Overlap: A Study Of New Zealand Schoolteachers, Renzo Bianchi, Irvin Sam Schonfeld, Eric Mayor, Eric Laurent

Publications and Research

We examined the overlap of burnout with depression in a sample of 184 New Zealand schoolteachers. Burnout and depressive symptoms were strongly correlated with each other (r = .73; disattenuated correlation: .82) and moderately correlated with dysfunctional attitudes, ruminative responses, and pessimistic attributions. All the participants with high frequencies of burnout symptoms were identified as clinically depressed. Suicidal ideation was reported by 36% of those participants. Three groups of teachers emerged from a two-step cluster analysis: “low burnout-depression,” “medium burnout-depression,” and “high burnout-depression.” The correlation between the affective-cognitive and somatic symptoms of depression was similar in strength to the burnout-depression …

Combining Body Mass And Shape Indices In Clinical Practice, Jesse C. Krakauer, Nir Y. Krakauer

Publications and Research

We present preliminary clinical experience with combined consideration of the commonly used BMI (body mass index) and the newly developed ABSI (a body shape index) using a point of care anthropometric calculator for comparisons of index values and associated relative risks to population normals. In a series of 282 patients, BMI and ABSI were close to being independently distributed, supporting the value of considering both indices. Three selected cases illustrate scenarios where assessment of ABSI together with BMI could inform patient care and counseling. These data suggest that combined assessment of BMI and ABSI may prove useful in clinical practice.

The Ethics And Politics Of Child Naming, Eldar Sarajlic

Publications and Research

This article examines the issue of justification of government’s intervention in the parental acts of child naming, a neglected topic in the recent philosophical literature. It questions the ability of some of the current theories in family ethics to respond to this problem and argues that both permissive and restrictive theories fail to provide a plausible argument about the proper limits of government regulation of child naming practices. The article outlines an alternative solution that focuses on the child’s right to authenticity and suggests that only those names that infringe upon this right invite justified state intervention.

Teaching Evolution: Blog As A Liminal Space, Shylaja Akkaraju, Alexander Wolf

Publications and Research

A threshold concept is a challenging concept that acts as a doorway leading to deeper understanding and a dramatic shift in perception. A learner that is in- volved in grasping a threshold concept is said to be undergoing a threshold expe- rience within a liminal space or learning environment. We used the blog as a lim- inal space for our students to engage with the theory of evolution, which is a threshold concept. In order to teach evolution effectively it is necessary to address both understanding and acceptance of evolutionary theory. To explore the latter in an introductory biology course, …

Daoism And Disability, Andrew Lambert

Publications and Research

Ideas found in the early Daoist texts can inform current debates about disability, since the latter often involve assumptions about personhood and agency that Daoist texts do not share. The two canonical texts of classical Daoism, the Daodejing and the Zhuangzi, do not explicitly discuss disability as an object of theory or offer a model of it. They do, however, provide conceptual resources that can enrich contemporary discussions of disability. Two particular ideas are discussed here. Classical Daoist thinking about the body undermines normative assumptions about it that attributions of ‘disabled’ often depend upon; and Daoism vividly problematises the …

A Review On Locomotor Training After Spinal Cord Injury: Reorganization Of Spinal Neuronal Circuits And Recovery Of Motor Function, Andrew C. Smith, Maria Knikou

Publications and Research

Locomotor training is a classic rehabilitation approach utilized with the aim of improving sensorimotor function and walking ability in people with spinal cord injury (SCI). Recent studies have provided strong evidence that locomotor training of persons with clinically complete, motor complete, or motor incomplete SCI induces functional reorganization of spinal neuronal networks at multisegmental levels at rest and during assisted stepping. This neuronal reorganization coincides with improvements in motor function and decreased muscle cocontractions. In this review, we will discuss the manner in which spinal neuronal circuits are impaired and the evidence surrounding plasticity of neuronal activity after locomotor training …

Caricature And Hyperbole In Preservice Teacher Professional Development, Mica Pollock, Candice Bocala, Sherry L. Deckman, Shari Dickstein-Staub

Publications and Research

Professional development (PD) “for diversity” aims to prepare teachers to support students from varying backgrounds to succeed, often in underresourced contexts. Although many teachers invite such inquiry as part of learning to teach, others resist “diversity” inquiry as extra to teaching, saying they cannot “do it all.” In this article, we discuss how preservice teachers at times caricature the requests of PD for diversity, hearing the task as a call to undertake superhuman tasks and to be people other than who they are. We argue that these caricatures require direct acknowledgment by both preservice teachers and teacher educators working in …

An Event-Related Potential Study Of Social Information Processing In Adolescents, Danielle Difilipo, Jillian Grose-Fifer

Publications and Research

Increased social awareness is a hallmark of adolescence. The primary aim of this eventrelated potential study was to investigate whether adolescents, in comparison to adults, would show relatively enhanced early neural processing of complex pictures containing socially-relevant information. A secondary aim was to investigate whether there are also gender and age differences in the ways adolescents and adults process social and nonsocial information. We recorded EEGs from 12–17 year-olds and 25–37 year-olds (N = 59) while they viewed pleasant pictures from the International Affective Picture System. We found age-related amplitude differences in the N1 and the LPP, and gender-related differences …

The Complicated Process Of Caregiving: The Case Of Mr. S (James) And Ms. Q (Sherry), Nicole Saint-Louis

Publications and Research

In the midst of the daily chaos of healthcare and hospital social work, there are tender moments shared with those we serve—moments that make it a privilege to share their journey. The story of two patients is recounted by the social worker that cared for them.

Abortion And The Right To Not Be Pregnant, James E. Mahon

Publications and Research

In this paper I defend Judith Jarvis Thomson's 'Good Samaritan Argument' (otherwise known as the 'feminist argument') for the permissibility of abortion, first advanced in her important, ground-breaking article 'A Defense of Abortion' (1971), against objections from Joseph Mahon (1979, 1984). I also highlight two problems with Thomson's argument as presented, and offer remedies for both of these problems. The article begins with a short history of the importance of the article to the development of practical ethics. Not alone did this article put the topic of the abortion on the philosophical map, but it made 'practical ethics' in the …

Killer-Cell Immunoglobulin-Like Receptor (Kir) Gene Profiles Modify Hiv Disease Course, Not Hiv Acquisition In South African Women, V. Naranbhai, D. De Assis Rosa, L. Werner, R. Moodley, H. Hong, A. Kharsany, K. Mlisana, S. Sibeko, N. Garrett, D. Chopera, William H. Carr, Q. Abdool Karim, A. V. S. Hill, S. S. Abdool Karim, M. Altfeld, C. M. Gray, T. Ndung’U

Publications and Research

Background: Killer-cell Immunoglobulin-like Receptors(KIR) interact with Human Leukocyte Antigen(HLA) to modify natural killer- and T-cell function. KIR are implicated in HIV acquisition by small studies that have not been widely replicated. A role for KIR in HIV disease progression is more widely replicated and supported by functional studies.

Methods: To assess the role of KIR and KIR ligands in HIV acquisition and disease course, we studied at-risk women in South Africa between 2004–2010. Logistic regression was used for nested case–control analysis of 154 women who acquired vs. 155 who did not acquire HIV, despite high exposure. Linear mixed-effects models were …

Gastrointestinal Safety, Chemotherapeutic Potential, And Classic Pharmacological Profile Of Nosh-Naproxen (Avt-219) A Dual No- And H2s-Releasing Hybrid, Mitali Chattopadhyay, Ravinder Kodela, Pascale L. Duvalsaint, Khosrow Kashfi

Publications and Research

Naproxen (NAP) is a potent nonsteroidal anti-inflammatory drug (NSAID) with a favorable cardiovascular profile. However, its long-term use may lead to serious gastrointestinal and renal side effects. NOSH- (nitric oxide and hydrogen sulfide) releasing naproxen (NOSH-NAP, AVT-219) belongs to a new class of anti-inflammatory agents designed to overcome these limitations. We compared the gastrointestinal safety, anti-inflammatory, analgesic, antipyretic, and antiplatelet properties of AVT-219 to that of NAP in preclinical animal models. We also evaluated its anticancer effects in 11 human cancer cell (HCC) lines of six different tissue origins and in a chemotherapeutic xenograft mouse model of colon cancer. AVT-219: …

The Self Is A Moving Target: The Neuroscience Of Siri Hustvedt's Artists, Jason Tougaw

Publications and Research

No abstract provided.

Comparative Phylogeography Of Ethiopian Anurans: Impact Of The Great Rift Valley And Pleistocene Climate Change, Xenia Freilich, Jose D. Anadon, Jolanta Bukala, Ordaliza Calderon, Ronveer Chakraborty, Stephanie Boissinot

Publications and Research

Background: The Ethiopian highlands are a biodiversity hotspot, split by the Great Rift Valley into two distinct systems of plateaus and mountains. The Rift Valley is currently hot and dry and acts as a barrier to gene flow for highland-adapted species. It is however unlikely that the conditions in the Rift were inhospitable to highland species during the entire Pleistocene. To assess the significance of the Ethiopian Rift as a biogeographic barrier as well as the impact Pleistocene climatic changes have had on the evolution of Ethiopian organisms, we performed phylogeographic analyses and developed present and past niche models on …

Non-Nad-Like Poly(Adp-Ribose) Polymerase-1 Inhibitors Effectively Eliminate Cancer In Vivo, Colin Thomas, Yingbiao Ji, Niraj Lodhi, Elena Kotova, Aaron Dan Pinnola, Konstantin Golovine, Peter Makhov, Kate Pechenkina, Vladimir Kolenko, Alexei V. Tulin

Publications and Research

The clinical potential of PARP-1 inhibitors has been recognized N10 years ago, prompting intensive research on their pharmacological application in several branches of medicine, particularly in oncology. However, natural or acquired resistance of tumors to known PARP-1 inhibitors poses a serious problemfor their clinical implementation. Present study aims to reignite clinical interest to PARP-1 inhibitors by introducing a new method of identifying highly potent inhibitors and presenting the largest known collection of structurally diverse inhibitors. The majority of PARP-1 inhibitors known to date have been developed as NAD competitors. NAD is utilized by many enzymes other than PARP-1, resulting in …

Within-Person Associations Between Daily Motivation And Self-Efficacy And Drinking Among Problem Drinkers In Treatment, Jon Morgenstern, Alexis Kuerbis, Jessica Houser, Frederick Muench, Sijing Shao, Hayley Treloar Padovano

Publications and Research

Gaining a better understanding of the change process holds promise to improve alcohol treatment. Ecological momentary assessment (EMA) coupled with intensive longitudinal data (ILD) approaches have been proposed as promising methods that can advance change process research but have been used infrequently in AUD treatment research. The current study used these approaches to examine the within-person associations of motivation and self-efficacy and drinking among treatment seeking problem drinkers. Participants (N=96) received daily EMA surveys before, during, and after treatment for seven weeks spread over a nine month period. Multi-level modeling was used to test the within-person relationships between the change …

Insights Into The Molecular Antiviral Mechanism Of Pokeweed Protein From Phytolacca Americana, Valentina R. Aitbakieva, Artem V. Domashevskiy

Publications and Research

Agriculture is an indispensable part of every person’s life, ensuring that nutritious and inexpensive food is readily available. As any other organisms, plants are subject to numerous parasitic infections. Biological evolution has allowed plants to produce a variety of toxic compounds to deal with their pathogens. American pokeweed plant (Phytolacca americana) manufactures pokeweed antiviral protein, a ribosome inactivating protein that disrupts protein synthesis and lowers infectivity of many plant and animal viruses. The intricate mechanism of PAP antiviral activity entails a delicate coordination and interplay of several factors, allowing the plant to battle its invaders. Here, we examine the molecular …

Cellmapper: Rapid And Accurate Inference Of Gene Expression In Difficult-To-Isolate Cell Types, Bradlee D. Nelms, Levi Waldron, Luis A. Barrera, Andrew W. Weflen, Jeremy A. Goettel, Guoji Guo, Robert K. Montgomery, Marian R. Neutra, David T. Breault, Scott B. Snapper, Stuart H. Orkin, Martha L. Bulyk, Curtis Huttenhower, Wayne I. Lencer

Publications and Research

We present a sensitive approach to predict genes expressed selectively in specific cell types, by searching publicly available expression data for genes with a similar expression profile to known cell-specific markers. Our method, CellMapper, strongly outperforms previous computational algorithms to predict cell type-specific expression, especially for rare and difficult-to-isolate cell types. Furthermore, CellMapper makes accurate predictions for human brain cell types that have never been isolated, and can be rapidly applied to diverse cell types from many tissues. We demonstrate a clinically relevant application to prioritize candidate genes in disease susceptibility loci identified by GWAS.

Moving Students To The Center Through Collaborative Documents In The Classroom, Maura A. Smale, Stephen Francoeur

Publications and Research

Collaborative document creation allows groups of people to create and edit text in a shared space, and educators across all subject areas have embraced these tools in their classes. Library instructors are no exception—the authors have used collaborative documents with students in multiple instructional settings. We believe that collaborative documents can embody critical pedagogy in the library classroom. Creating and editing collaborative documents can acknowledge students’ prior experiences with research and the library and de-center the library instructor as the sole research expert in the room.

Natural Cultch Type Influences Habitat Preference And Predation, But Not Survival, In Reef-Associated Species, Erica A. Levine, Stephen Gosnell J., Emily M. Goetz, Christopher R. Malinowski

Publications and Research

A shared origin with fresh and dredged cultch and availability via mining have made fossil cultch a commonly used reef restoration substrate. However, important differences in shape and size between whole-shell cultch and fossil cultch may impact the complexity of reefs constructed from these materials. To determine if these differences may impact the development of restored reefs, we quantified the interstitial space each cultch type provides and constructed reef mesocosms to measure (1) the immediate effects of exposure to each cultch type on mortality of blue crab (Callinectes sapidus) and pink shrimp (Farfantepenaeus duorarum); (2) the …

In Search Of A Grand Narrative: The Turbulent History Of Teaching, Judith R. Kafka

Publications and Research

For this review of research on the history of teaching, I use the instructional triangle as an organizing tool and frame of analysis to explore what we know about who taught, who was taught, and what was taught across space and time.

In the first section of this chapter I review historical research on who taught in American classrooms. One overwhelming theme throughout this literature is that policy makers, school leaders, and the general public have historically cared a great deal about who a teacher was, often basing their preferences on the belief that a teacher’s social characteristics would shape …

Unworking Milton: Steps To A Georgics Of The Mind, Steven Swarbrick

Publications and Research

Traditionally read as a poem about laboring subjects who gain power through abstract and abstracting forms of bodily discipline, John Milton’s Paradise Lost (1667, 1674) more compellingly foregrounds the erotics of the Garden as a space where humans and nonhumans intra-act materially and sexually. Following Christopher Hill, who long ago pointed to not one but two revolutions in the history of seventeenth-century English radicalism—the first, ‘the one which succeeded[,] . . . the protestant ethic’; and the second, ‘the revolution which never happened,’ which sought ‘communal property, a far wider democracy[,] and rejected the protestant ethic’—I show how Milton’s Paradise …