Digital Commons Network^™

Examining The Safety Of Respiratory And Intravenous Inoculation Of Bdellovibrio Bacteriovorus And Micavibrio Aeruginosavorus In A Mouse Model, Kenneth Shatzkes, Richard Chae, Chi Tang, Gregory C. Ramirez, Somdatta Mukherjee, Liana Tsenova, Nancy D. Connell, Daniel E. Kadouri

Publications and Research

Bdellovibrio spp. and Micavibrio spp. are Gram-negative predators that feed on other Gram-negative bacteria, making predatory bacteria potential alternatives to antibiotics for treating multi-drug resistant infections. While the ability of predatory bacteria to control bacterial infections in vitro is well documented, the in vivo effect of predators on a living host has yet to be extensively examined. In this study, respiratory and intravenous inoculations were used to determine the effects of predatory bacteria in mice. We found no reduction in mouse viability after intranasal or intravenous inoculation of B. bacteriovorus 109J, HD100 or M. aeruginosavorus. Introducing predators into the respiratory …

Tailored Delivery Of Analgesic Ziconotide Across A Blood Brain Barrier Model Using Viral Nanocontainers, Prachi Anand, Alison O’Neil, Emily Lin, Trevor Douglas, Mandë Holford

Publications and Research

The blood brain barrier (BBB) is often an insurmountable obstacle for a large number of candidate drugs, including peptides, antibiotics, and chemotherapeutic agents. Devising an adroit delivery method to cross the BBB is essential to unlocking widespread application of peptide therapeutics. Presented here is an engineered nanocontainer for delivering peptidic drugs across the BBB encapsulating the analgesic marine snail peptide ziconotide (Prialt®). We developed a bi-functional viral nanocontainer based on the Salmonella typhimurium bacteriophage P22 capsid, genetically incorporating ziconotide in the interior cavity, and chemically attaching cell penetrating HIV-Tat peptide on the exterior of the capsid. Virus like particles (VLPs) …

Geospatial Resolution Of Human And Bacterial Diversity With City-Scale Metagenomics, Ebrahim Afshinnekoo, Cem Meydan, Shanin Chowdhury, Dyala Jaroudi, Collin Boyer, Nick Bernstein, Julia M. Maritz, Darryl Reeves, Jorge Gandara, Sagar Chhangawala, Sofia Ahsanuddin, Amber Simmons, Timothy Nessel, Bharathi Sundaresh, Elizabeth Pereira, Ellen Jorgensen, Sergios-Orestis Kolokotronis, Nell Kirchberger, Isaac Garcia, David Gandara, Sean Dhanraj, Tanzina Nawrin, Yogesh Saletore, Noah Alexander, Priyanka Vijay, Elizabeth M. Hénaff, Paul Zumbo, Michael Walsh, Gregory D. O'Mullan, Scott Tighe, Joel T. Dudley, Anya Dunaif, Sean Ennis, Eoghan O'Halloran, Tiago R. Magalhaes, Braden Boone, Angela L. Jones, Theodore R. Muth, Katie Schneider Paolantonio, Elizabeth Alter, Eric E. Schadt, Jeanne Garbarino, Robert J. Prill, Jane M. Carlton, Shawn Levy, Christopher E. Mason

Publications and Research

The panoply of microorganisms and other species present in our environment influence human health and disease, especially in cities, but have not been profiled with metagenomics at a city-wide scale. We sequenced DNA from surfaces across the entire New York City (NYC) subway system, the Gowanus Canal, and public parks. Nearly half of the DNA (48%) does not match any known organism; identified organisms spanned 1,688 bacterial, viral, archaeal, and eukaryotic taxa, which were enriched for harmless genera associated with skin (e.g., Acinetobacter). Predicted ancestry of human DNA left on subway surfaces can recapitulate U.S. Census demographic data, and bacterial …

Serving The Commuter College Student In Urban Academic Libraries, Mariana Regalado, Maura A. Smale

Publications and Research

Cities often host many colleges and universities; while the commuter student in suburban or rural areas may drive or be driven to school, students at colleges and universities in dense, urban settings rely predominantly on mass transit for their commute to class. The act of commuting to campus has been found by a number of researchers to define and shape the experiences of commuter students in college, though the literature on college students who commute is not extensive. A qualitative study of the academic culture and scholarly habits of undergraduate students at the City University of New York (CUNY) revealed …

Social Media Use And Hiv Transmission Risk Behavior Among Ethnically Diverse Hiv-Positive Gay Men: Results Of An Online Study In Three U.S. States, Sabina Hirshfield, Christian Grov, Jeffrey T. Parsons, Ian Anderson, Mary Ann Chiasson

Publications and Research

Though Black and Hispanic men who have sex with men (MSM) are at an increased risk for HIV, few HIV risk reduction interventions that target HIV-positive MSM, and even fewer that use technology, have been designed to target these groups. Despite similar rates of social media and technology use across racial/ethnic groups, online engagement of minority MSM for HIV prevention efforts is low. Since minority MSM tend to have less representation in online HIV prevention studies, the goals of this online anonymous study of HIV-positive gay-identified men were to test the feasibility of conducting targeted recruitment by race/ethnicity and sexual …

Cdc6 Degradation Requires Phosphodegron Created By Gsk-3 And Cdk1 For Scfcdc4 Recognition In Saccharomyces Cerevisiae, Amr Al-Zain, Lea Schroeder, Alina Sheglov, Amy E. Ikui

Publications and Research

DNA replication has to be tightly regulated to ensure genome integrity such that DNA replication takes place only once per cell cycle. The Cdc6 sequential phosphorylation by GSK-3 and Cdk1 creates a binding site for Cdc4 ubiquitin ligase to promote Cdc6 degradation.

Common Cell Shape Evolution Of Two Nasopharyngeal Pathogens, Frédéric J. Veyrier, Nicolas Biais, Pablo Morales, Nouria Belkacem, Cyril Guilhen, Sylvia Ranjeva, Odile Sismeiro, Gérard Péhau-Arnaudet, Eduardo P. Rocha, Catherine Werts, Muhamed-Kheir Taha, Ivo G. Boneca

Publications and Research

Respiratory infectious diseases are the third cause of worldwide death. The nasopharynx is the portal of entry and the ecological niche of many microorganisms, of which some are pathogenic to humans, such as Neisseria meningitidis and Moraxella catarrhalis. These microbes possess several surface structures that interact with the actors of the innate immune system. In our attempt to understand the past evolution of these bacteria and their adaption to the nasopharynx, we first studied differences in cell wall structure, one of the strongest immune-modulators. We were able to show that a modification of peptidoglycan (PG) composition (increased proportion of pentapeptides) …

Nitric Oxide-Releasing Aspirin Suppresses Nf-Κb Signaling In Estrogen Receptor Negative Breast Cancer Cells In Vitro And In Vivo, Niharika Nath, Mitali Chattopadhyay, Deborah B. Rodes, Anna Nazarenko, Ravinder Kodela, Khosrow Kashfi

Publications and Research

Estrogen receptor negative (ER(−)) breast cancer is aggressive, responds poorly to current treatments and has a poor prognosis. The NF-κB signaling pathway is implicated in ER(−) tumorigenesis. Aspirin (ASA) is chemopreventive against ER(+) but not for ER(−) breast cancers. Nitric oxide-releasing aspirin (NO-ASA) is a safer ASA where ASA is linked to an NO-releasing moiety through a spacer. In vitro, we investigated anti-proliferation effects of NO-ASA (para- and meta-isomers) against ER(−) breast cancer cells MDA-MB-231 and SK-BR-23, effects on NF-κB signaling, and reactive oxygen species by standard techniques. In vivo, effects of NO-ASA were evaluated in a mouse xenograft model …

Transdisciplinarity: A Review Of Its Origins, Development, And Current Issues, Jay H. Bernstein

Publications and Research

Transdisciplinarity originated in a critique of the standard configuration of knowledge in disciplines in the curriculum, including moral and ethical concerns. Pronouncements about it were first voiced between the climax of government-supported science and higher education and the long retrenchment that began in the 1970s. Early work focused on questions of epistemology and the planning of future universities and educational programs. After a lull, transdisciplinarity re-emerged in the 1990s as an urgent issue relating to the solution of new, highly complex, global concerns, beginning with climate change and sustainability and extending into many areas concerning science, technology, social problems and …

The Interaction Between Fluid Wall Shear Stress And Solid Circumferential Strain Affects Endothelial Gene Expression, Ronny Amaya, Alexis Pierides, John M. Tarbell

Publications and Research

Endothelial cells lining the walls of blood vessels are exposed simultaneously to wall shear stress (WSS) and circumferential stress (CS) that can be characterized by the temporal phase angle between WSS and CS (stress phase angle – SPA). Regions of the circulation with highly asynchronous hemodynamics (SPA close to -180°) such as coronary arteries are associated with the development of pathological conditions such as atherosclerosis and intimal hyperplasia whereas more synchronous regions (SPA closer to 0°) are spared of disease. The present study evaluates endothelial cell gene expression of 42 atherosclerosis-related genes under asynchronous hemodynamics (SPA=-180 °) and synchronous hemodynamics …

Cyclometalated Iminophosphorane Gold(Iii) And Platinum(Ii) Complexes. A Highly Permeable Cationic Platinum(Ii) Compound With Promising Anticancer Properties, Malgorzata Frik, Jacob Fernández-Gallardo, Oscar Gonzalo, Víctor Mangas-Sanjuan, Marta González-Alvarez, Alfonso Serrano Del Valle, Chunhua Hu, Isabel González-Alvarez, Marival Bermejo, Isabel Marzo, María Contel

Publications and Research

New organometallic gold(III) and platinum(II) complexes containing iminophosphorane ligands are described. Most of them are more cytotoxic to a number of human cancer cell lines than cisplatin. Cationic Pt(II) derivatives 4 and 5, which differ only in the anion, Hg2Cl62– or PF6– respectively, display almost identical IC50 values in the sub-micromolar range (25–335-fold more active than cisplatin on these cell lines). The gold compounds induced mainly caspase-independent cell death, as previously reported for related cycloaurated compounds containing IM ligands. Cycloplatinated compounds 3, 4, and 5 can also activate alternative caspase-independent mechanisms of death. However, at short incubation times cell death …

Rationale, Design And Respondent Characteristics Of The 2013–2014 New York City Health And Nutrition Examination Survey (Nyc Hanes 2013–2014), Lorna E. Thorpe, Carolyn Greene, Amy Freeman, Elisabeth Snell, Jesica S. Rodriguez-Lopez, Martin Frankel, Amado Punsalang Jr., Claudia Chernov, Elizabeth Lurie, Mark Friedman, Ram Koppaka, Sharon E. Perlman

Publications and Research

Purpose. Capacity to monitor non-communicable diseases (NCDs) at state or local levels is limited. Emerging approaches include using biomeasures and electronic health record (EHR) data. In 2004, New York City (NYC) performed a population-based health study on adult residents using biomeasures (NYC Health and Nutrition Examination Study, or NYC HANES), modeled after NHANES. A second NYC HANES was launched in 2013 to examine change over time, evaluate municipal policies, and validate a proposed EHR-based surveillance system. We describe the rationale and methods of NYC HANES 2013–2014.

Methods. NYC HANES was a population-based, cross-sectional survey of NYC adults using three-stage cluster …

Catching Up With Time: Tips, Tricks, And Best Practices For Library Renovations, Simone L. Yearwood

Publications and Research

A personal narrative is presented which explores the author's experience of leading the renovation of the Benjamin S. Rosenthal Library (BRL) at Queens College of the City University of New York in New York City.

Inhibition Of Cell Differentiation In Bacillus Subtilis By Pseudomonas Protegens, Matthew J. Powers, Edgardo Sanabria-Valentín, Albert A. Bowers, Elizabeth A. Shanka

Publications and Research

Interspecies interactions have been described for numerous bacterial systems, leading to the identification of chemical compounds that impact bacterial physiology and differentiation for processes such as biofilm formation. Here, we identified soil microbes that inhibit biofilm formation and sporulation in the common soil bacterium Bacillus subtilis. We did so by creating a reporter strain that fluoresces when the transcription of a biofilm-specific gene is repressed. Using this reporter in a coculture screen, we identified Pseudomonas putida and Pseudomonas protegens as bacteria that secrete compounds that inhibit biofilm gene expression in B. subtilis. The active compound produced by P. protegens was …

Science And Charity: Rival Catholic Visions For Humanitarian Practice At The End Of French Rule In Cameroon, Charlotte Walker-Said

Publications and Research

This paper explores the conflict between local expressions of Christian charity and new theories of scientific humanitarianism in the final years of French rule in Africa. Compassionate phenomena inspired by Catholic social organizing had transformed everyday life throughout French Cameroon’s cities and villages in the interwar and postwar years, and yet, in 1950, poverty, crime, poor public health, and social tensions remained prevalent. Seeking a more deeply transformative approach to social rehabilitation, ecclesiastical leaders in the Catholic Church in Europe and French foreign missionary societies in Africa partnered with international medical and scientific organizations in order to invigorate charity with …

Familiarity With And Preferences For Oral And Long-Acting Injectable Hiv Pre-Exposure Prophylaxis (Prep) In A National Sample Of Gay And Bisexual Men In The U.S., Jeffrey T. Parsons, H. Jonathan Rendina, Thomas H.F. Whitfield, Christian Grov

Publications and Research

Objectives: We sought to determine preferences for oral versus long-acting injectable (LAI) PrEP among gay and bisexual men (GBM).

Methods: We surveyed a national U.S. sample of 1,071 GBM about forms of PrEP.

Results: Overall, 46.0% preferred LAI, 14.3% oral, 21.7% whichever was most effective, 10.1% had no preference, and 7.8% would not take PrEP. There were no differences in PrEP preferences by race/ethnicity, income, region of residence, or relationship status. Those unwilling to take PrEP were significantly older than those who preferred LAI PrEP and those who would take either. Those who preferred the most effective …

Homophily, Close Friendship, And Life Satisfaction Among Gay, Lesbian, Heterosexual, And Bisexual Men And Women, Brian Joseph Gillespie, David Frederick, Lexi Harari, Christian Grov

Publications and Research

Friends play important roles throughout our lives by providing expressive, instrumental, and companionate support. We examined sexual orientation, gender, and age differences in the number of friends people can rely on for expressive, instrumental, and companionate support. Additionally, we examined the extent to which people relied on same-gender versus cross-gender friends for these types of support. Participants (N = 25,185) completed a survey via a popular news website. Sexual orientation differences in number of same-gender and cross-gender friends were generally small or non-existent, and satisfaction with friends was equally important to overall life satisfaction for all groups. However, the extent …

Collecting Cats: Library Lessons From Neko Atsume, Kelly M. Blanchat, Megan Brooks

Publications and Research

This blog post is the culmination of a Twitter conversation between librarians talking about their experiences playing a phone game. The game is called Nekoatsume and it involves taking care of digital cats in a virtual backyard. Nekoatsume is entirely in Japanese, a key fact that actually started the Twitter conversation (and not the fact that the game involves cats, as might be expected). Despite the language barrier, Nekoatsume is remarkably user-friendly; library databases should be just as user-friendly as a game in a foreign language, but too often they’re not. With so many variables in acquiring research — design, …

Mobilisation Of Public Support For Policy Actions To Prevent Obesity, Terry T-K Huang, John H. Cawley, Marice Ashe, Sergio Costa, Leah M. Frerichs, Lindsey Zwicker, Juan A. Rivera, David Levy, Ross A. Hammond, Estelle V. Lambert, Shiriki K. Kumanyika

Publications and Research

Public mobilisation is needed to enact obesity prevention policies and to mitigate backlash against their implementation. However, current approaches in public health focus primarily on dialogue between public health professionals and political leaders. Strategies to increase popular demand for obesity prevention policies include refining and streamlining public information, identifying effective frames for each population, enhancing media advocacy, building citizen protest and engagement, and developing a receptive political environment with change agents embedded across organisations and sectors. Long-term support and investment in collaboration among diverse stakeholders to create shared value is also important. Each actor in an expanded coalition for obesity …

The Mission As A Master Signifier: Documentary Film, Social Change And Discourse Analysis, Joseph Van Der Naald

Publications and Research

This field note explores the ways in which the documentary MIND ZONE: Therapists Behind the Front Lines (2014), directed by Dr. Jan Haaken, moves viewers into innovative and critical stances towards the U.S. military mental health program. Using a discourse analysis of the film and transcripts of interviews conducted by Haaken, I trace the deployment of the term “the mission” to show how the film teases apart problematic military discursive practices. Jacques Lacan’s theory of the four discourses is used to analyze how MIND ZONE’s content challenges audiences to produce their own critically informed opinions.

Perceptions Of Violence In The South Bronx, Jeffrey A. Butts, Sheyla A. Delgado, Marissa Mandala

Publications and Research

The NYCCure study measured changes in violent norms and attitudes in areas of New York City operating Cure Violence programs. Respondents were men aged 18-30 from the South Bronx.

Perceptions Of Violence In East New York (Brooklyn), Sheyla A. Delgado, Jeffrey A. Butts, Marissa Mandala

Publications and Research

The NYCCure study measured changes in violent norms and attitudes in areas of New York City operating Cure Violence programs. Respondents were men aged 18-30 from the East New York area of Brooklyn.

Perceptions Of Violence In Harlem, Sheyla A. Delgado, Jeffrey A. Butts, Marissa Mandala

Publications and Research

The NYCCure study measured changes in violent norms and attitudes in areas of New York City operating Cure Violence programs. Respondents were men aged 18-30 from the East Harlem area of Manhattan.

Evolutionary Dynamics Of Rhomboid Proteases In Streptomycetes, Peter A. Novick, Naydu M. Carmona, Monica Trujillo

Publications and Research

Background Proteolytic enzymes are ubiquitous and active in a myriad of biochemical pathways. One type, the rhomboids are intramembrane serine proteases that release their products extracellularly. These proteases are present in all forms of life and their function is not fully understood, although some evidence suggests they participate in cell signaling. Streptomycetes are prolific soil bacteria with diverse physiological and metabolic properties that respond to signals from other cells and from the environment. In the present study, we investigate the evolutionary dynamics of rhomboids in Streptomycetes, as this can shed light into the possible involvement of rhomboids in the complex …

Kinetic Study Of The Oxygen Reduction Reaction On Α-Ni(Oh)2 And Α-Ni(Oh)2 Supported On Graphene Oxide, Elaheh Farjami, L. Jay Deiner

Publications and Research

The kinetics of the oxygen reduction reaction on α-Ni(OH)2 and α-Ni(OH)2 supported on graphene oxide (α-Ni(OH)2/GO) were investigated using rotating disk linear sweep voltammetry in alkaline solutions of varying oxygen and hydroxyl concentrations. Over the full hydroxyl concentration range (0.05 M to 0.5M), α-Ni(OH)2/GO displayed higher activity than unsupported α-Ni(OH)2. The electron transfer numbers were 2.9 ± 0.2 for α-Ni(OH)2, 3.4 ± 0.1 for α-Ni(OH)2/GO at low [OH−], and 3.8–3.9 for α-Ni(OH)2/GO at high [OH−]. Compared to α-Ni(OH)2, α-Ni(OH)2/GO displayed higher chemical reaction rate constants and higher electron transfer rate constants. These differences suggest that the synergy between the α-Ni(OH)2 …

Quantitative Analyses Of Force-Induced Amyloid Formation In Candida Albicans Als5p: Activation By Standard Laboratory Procedures, Cho X. J. Chan, Ivor G. Joseph, Andy Huang, Desmond N. Jackson, Peter N. Lipke

Publications and Research

Candida albicans adhesins have amyloid-forming sequences. In Als5p, these amyloid sequences cluster cell surface adhesins to create high avidity surface adhesion nanodomains. Such nanodomains form after force is applied to the cell surface by atomic force microscopy or laminar flow. Here we report centrifuging and resuspending S. cerevisiae cells expressing Als5p led to 1.7-fold increase in initial rate of adhesion to ligand coated beads. Furthermore, mechanical stress from vortex-mixing of Als5p cells or C. albicans cells also induced additional formation of amyloid nanodomains and consequent activation of adhesion. Vortex-mixing for 60 seconds increased the initial rate of adhesion 1.6-fold. The …

Social Media As Game Strategy: Twitter In The #Infolit Instruction Session, Kelly M. Blanchat, Lydia Willoughby

Publications and Research

The lure of distractions can entice even the strongest of student wills in a computer classroom. Research requires strategic thinking and ordered planning to drown out the noise of online distractions. This poster demonstrates a unique way to capitalize on the natural overlap of research, communication, and social media by employing game strategy to lead learning outcomes for undergraduate student research. Instead of silencing social media, this activity incorporates Twitter as a platform to introduce information literacy concepts and participatory practices of scholarship.

Engage Students Through Flipped Instruction: A Lesson Creation Lab, Madeline Cohen, Alison Lehner-Quam, Robin Wright

Publications and Research

Presentation at 2015 SUNYLA Conference, June 3, 2015.

Embedded Librarian Ideas: Best Practices Explored And Redefined, Carl R. Andrews

Publications and Research

This paper explores the multitude of ways in which embedded librarianship can be implemented. Although the paper is primarily targeted to academic librarians in higher education settings, the literature examined and the ideas presented can support secondary and college level inter-disciplinary teaching initiatives. The ideas presented are sourced from scholarly journal articles, monographs, and best practices implemented by the author. Attention is given to programs where Information Literacy is infused into a school’s General Education curriculum. Academic librarian pedagogy, outreach, and networking strategies are also highlighted. The author is especially interested in projects that address student academic success after an …

The Broken Ring: Reduced Aromaticity In Lys-Trp Cations And High Ph Tautomer Correlates With Lower Quantum Yield And Shorter Lifetimes, Azaria Solomon Eisenberg, Laura J. Juszczak

Publications and Research

Several nonradiative processes compete with tryptophan fluorescence emission. The difficulty in spectral interpretation lies in associating specific molecular environmental features with these processes and thereby utilizing the fluorescence spectral data to identify the local environment of tryptophan. Here, spectroscopic and molecular modeling study of Lys-Trp dipeptide charged species shows that backbone-ring interactions are undistinguished. Instead, quantum mechanical ground state isosurfaces reveal variations in indole π electron distribution and density that parallel charge (as a function of pK1, pK2, and pKR) on the backbone and residues. A pattern of aromaticity-associated quantum yield and fluorescence lifetime changes emerges. Where quantum yield is …