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Integration Of Subthreshold And Suprathreshold Excitatory Barrages Along The Somatodendritic Axis Of Pyramidal Neurons, Hysell V. Oviedo, Alex D. Reyes

Publications and Research

Neurons integrate inputs arriving in different cellular compartments to produce action potentials that are transmitted to other neurons. Because of the voltage- and time-dependent conductances in the dendrites and soma, summation of synaptic inputs is complex. To examine summation of membrane potentials and firing rates, we performed whole-cell recordings from layer 5 cortical pyramidal neurons in acute slices of the rat’s somatosensory cortex. We delivered subthreshold and suprathreshold stimuli at the soma and several sites on the apical dendrite, and injected inputs that mimic synaptic barrages at individual or distributed sites. We found that summation of subthreshold potentials differed from …

Rational Design And Characterization Of D-Phe-Pro-D-Arg-Derived Direct Thrombin Inhibitors, Ana C. Figueiredo, Christina C. Clement, Sheuli Zakia, Julian Gingold, Manfred Philipp, Pedro J.B. Pereira

Publications and Research

The tremendous social and economic impact of thrombotic disorders, together with the considerable risks associated to the currently available therapies, prompt for the development of more efficient and safer anticoagulants. Novel peptide-based thrombin inhibitors were identified using in silico structure-based design and further validated in vitro. The best candidate compounds contained both l- and d-amino acids, with the general sequence d-Phe(P3)-Pro(P2)-d-Arg(P1)-P1'-CONH2. The P1' position was scanned with l- and d-isomers of natural or unnatural amino acids, covering the major chemical classes. The most potent non-covalent and proteolysis-resistant inhibitors contain small hydrophobic or polar amino acids (Gly, Ala, Ser, Cys, Thr) …

Understanding The Public Health Challenges In The Era Of Mass Incarceration. President Travis' Keynote Speech At The Fifth Annual Academic And Health Policy Conference On Correctional Health On March 22, 2012 In Atlanta, Ga., Jeremy Travis

Publications and Research

No abstract provided.

Advances In Ultrafast Time Resolved Fluorescence Physics For Cancer Detection In Optical Biopsy, R. R. Alfano

Publications and Research

We discuss the use of time resolved fluorescence spectroscopy to extract fundamental kinetic information on molecular species in tissues. The temporal profiles reveal the lifetime and amplitudes associated with key active molecules distinguishing the local spectral environment of tissues. The femtosecond laser pulses at 310 nm excite the tissue. The emission profile at 340 nm from tryptophan is non-exponential due to the micro-environment. The slow and fast amplitudes and lifetimes of emission profiles reveal that cancer and normal states can be distinguished. Time resolved optical methods offer a new cancer diagnostic modality for the medical community.

Demonstration Of The Gunnarsson-Lundqvist Theorem And The Multiplicity Of Potentials For Excited States, Yu-Qi Li, Xiao-Yin Pan, Biao Li, Viraht Sahni

Publications and Research

The Gunnarsson-Lundqvist (GL) theorem of density functional theory states that there is a one-to-one relationship between the density of the lowest nondegenerate excited state of a given symmetry and the external potential. As a consequence, knowledge of this excited state density determines the external potential uniquely. [The GL theorem is the equivalent for such excited states of theHohenberg-Kohn (HK) theorem for nondegenerate ground states.] For other excited states, there is no equivalent of the GL or HK theorem. For these states, there thus exist multiple potentials that generate the excited-state density. We show, by example, the satisfaction that the GL …

Fellow Travellers: A Concordance Of Colonization Patterns Between Mice And Men In The North Atlantic Region, E P. Jones, K Skirnisson, T H. Mcgovern, Mtp Gilbert, E Willerslev, J B. Searle

Publications and Research

Background House mice (Mus musculus) are commensals of humans and therefore their phylogeography can reflect human colonization and settlement patterns. Previous studies have linked the distribution of house mouse mitochondrial (mt) DNA clades to areas formerly occupied by the Norwegian Vikings in Norway and the British Isles. Norwegian Viking activity also extended further westwards in the North Atlantic with the settlement of Iceland, short-lived colonies in Greenland and a fleeting colony in Newfoundland in 1000 AD. Here we investigate whether house mouse mtDNA sequences reflect human history in these other regions as well. Results House mice samples from Iceland, whether …

Mobile Information Literacy Let’S Use An App For That!, Stefanie Havelka, Alevtina Verbovetskaya

Publications and Research

Presentation at 3Ts 2012: Engaging Students with Transliteracy, Technology, and Teaching conference,

Transfer Of Juveniles To Criminal Court Is Not Correlated With Falling Youth Violence, Jeffrey A. Butts

Publications and Research

Though it may seem that transferring youth to criminal court should lower youth violent crime, this analysis shows no relationship. This databit looks at the transfer rate and violent crime declines in the six states that reported juvenile arrests to the FBI.

Sumoylation Is Tumor-Suppressive And Confers Proliferative Quiescence To Hematopoietic Progenitors In Drosophila Melanogaster Larvae, Marta E. Kalamarz, Indira Paddibhatla, Christina Nadar, Shubha Govind

Publications and Research

Summary How cell-intrinsic regulation of the cell cycle and the extrinsic influence of the niche converge to provide proliferative quiescence, safeguard tissue integrity, and provide avenues to stop stem cells from giving rise to tumors is a major challenge in gene therapy and tissue engineering. We explore this question in sumoylation-deficient mutants of Drosophila. In wild type third instar larval lymph glands, a group of hematopoietic stem/progenitor cells acquires quiescence; a multicellular niche supports their undifferentiated state. However, how proliferative quiescence is instilled in this population is not understood. We show that Ubc9 protein is nuclear in this population. Loss …

Using Equivalence-Based Instruction To Increase Efficiency In Teaching Neuroanatomy, Carolyn L. Pytte, Daniel M. Fienup

Publications and Research

A goal of all instruction is to efficiently allocate time spent teaching -- balancing redundancy that enhances learning with redundancy that is irrelevant to increasing student understanding. Efficient allocation of time allows the instructor to present additional material and go into more detail about the information being presented. Here we borrow laboratory research on concept formation and apply these formal principles in teaching introductory neuroanatomy within a lecture course on Behavioral Neuroscience. Concept formation is taught by pairing multiple stimuli, for instance brain name, location, and function, in such a way that novel associations within a category emerge without direct …

In Pursuit Of The Individual In The Field Of Knowledge Management, Isabel D. W. Rechberg, Jawad Syed

Publications and Research

The aim of this paper is to explore the focus on individuals in the field of knowledge management (KM). Through a meta-review of the KM literature, we identify a relative disregard of the individual in the KM literature while information technology (IT) oriented concepts are widely represented. Our review indicates the need for a greater emphasis on individuals in KM as knowledge is based on individuals’ ability and willingness to create, share and transfer knowledge. We offer suggestions on how to integrate individuals into theorising and enacting KM and also identify some avenues for future research.

Genome Stability Of Lyme Disease Spirochetes: Comparative Genomics Of Borrelia Burgdorferi Plasmids, Sherwood R. Casjens, Emmanuel F. Mongodin, Wei-Gang Qiu, Benjamin J. Luft, Steven E. Schutzer, Eddie B. Gilcrease, Wai Mun Huang, Marija Vujadinovic, John K. Aron, Levy C. Vargas, Sam Freeman, Diana Radune, Janice F. Weidman, George I. Dimitrov, Hoda M. Khouri, Julia E. Sosa, Rebecca A. Halpin, John J. Dunn, Claire M. Fraser

Publications and Research

Lyme disease is the most common tick-borne human illness in North America. In order to understand the molecular pathogenesis, natural diversity, population structure and epizootic spread of the North American Lyme agent, Borrelia burgdorferi sensu stricto, a much better understanding of the natural diversity of its genome will be required. Towards this end we present a comparative analysis of the nucleotide sequences of the numerous plasmids of B. burgdorferi isolates B31, N40, JD1 and 297. These strains were chosen because they include the three most commonly studied laboratory strains, and because they represent different major genetic lineages and so are …

Entropy And The Complexity Of Graphs Revisited, Abbe Mowshowitz, Matthias Dehmer

Publications and Research

This paper presents a taxonomy and overview of approaches to the measurement of graph and network complexity. The taxonomy distinguishes between deterministic (e.g., Kolmogorov complexity) and probabilistic approaches with a view to placing entropy-based probabilistic measurement in context. Entropy-based measurement is the main focus of the paper. Relationships between the different entropy functions used to measure complexity are examined; and intrinsic (e.g., classical measures) and extrinsic (e.g., Körner entropy) variants of entropy-based models are discussed in some detail.

A Cross-Sectional Description Of Social Capital In An International Sample Of Persons Living With Hiv/Aids (Plwh), Allison Webel, J Craig Phillips, Carol Dawson Rose, William L. Holzemer, Wei-Ti Chen, Lynda Tyer-Viola, Marta Rivero-Méndez, Patrice Nicholas, Kathleen Nokes, Jeanne Kemppainen, Elizabeth Sefcik, John Brion, Lucille Eller, Scholastika Iipinge, Kenn Kirksey, Dean Wantland, Puangtip Chaiphibalsarisdi, Mallory O. Johnson, Carmen Portillo, Inge B. Corless

Publications and Research

Background Social capital refers to the resources linked to having a strong social network. This concept plays into health outcomes among People Living with HIV/AIDS because, globally, this is a highly marginalized population. Case studies show that modifying social capital can lead to improvements in HIV transmission and management; however, there remains a lack of description or definition of social capital in international settings. The purpose of our paper was to describe the degree of social capital in an international sample of adults living with HIV/AIDS. Methods We recruited PLWH at 16 sites from five countries including Canada, China, Namibia, …

The Logic Of Fashion Cycles, Alberto Acerbi, Stefano Ghirlanda, Magnus Enquist

Publications and Research

Many cultural traits exhibit volatile dynamics, commonly dubbed fashions or fads. Here we show that realistic fashion-like dynamics emerge spontaneously if individuals can copy others' preferences for cultural traits as well as traits themselves. We demonstrate this dynamics in simple mathematical models of the diffusion, and subsequent abandonment, of a single cultural trait which individuals may or may not prefer. We then simulate the coevolution between many cultural traits and the associated preferences, reproducing power-law frequency distributions of cultural traits (most traits are adopted by few individuals for a short time, and very few by many for a long time), …

Queensborough Community College: Textbook Reserves, Sheila Beck

Publications and Research

This paper examines the textbook reserve collection at Queensborough Community College. Although the collection is heavily used in its current configuration, the library is looking for less labor intensive and less costly alternatives.

The cost of textbooks is skyrocketting. Textbooks are a major, unexpected bill for many students beginning college. Students and their family, used to having their textbooks provided or loaned free of charge at the start of the school year, face often large and unexpected bills when the first term begins. Many times, financial aid does not cover the cost of the books;. Even if it is covered …

The Docks Of New York, William Blick

Publications and Research

Josef Von Sternberg was a master film craftsman. Known for its use of shadow and closeup, The Docks of New York, demonstrates the work of a filmmaker at the peak of his powers.

This article was written for Senses of Cinema magazine to accompany the screenings at the Cinemateque in Melbourne, Australia.

Power Of The Narrator (Review), Veronica Schanoes

Publications and Research

Reviews the book Narrative Power: Encounters, Celebrations, Struggles by L. Timmel Duchamp. Seattle, WA: Aqueduct, 2010

School Crime Has Declined Sharply Since The 1990'S, Jeffrey A. Butts

Publications and Research

Between 1994 to 2010, the rate of violent victimizations at American schools dropped, with a sharp fall after 1994. This databit looks at property and violent crimes in schools from the early 1990s to 2010.

Genetic Reporter System For Positioning Of Proteins At The Bacterial Pole, Kathryn R. Fixen, Anuradha Janakiraman, Sean Garritty, Daniel J. Slade, Andrew N. Gray, Nilay Karahan, Ann Hochschild, Marcia C. Goldberg

Publications and Research

Spatial organization within bacteria is fundamental to many cellular processes, although the basic mechanisms underlying localization of proteins to specific sites within bacteria are poorly understood. The study of protein positioning has been limited by a paucity of methods that allow rapid large-scale screening for mutants in which protein positioning is altered. We developed a genetic reporter system for protein localization to the pole within the bacterial cytoplasm that allows saturation screening for mutants in Escherichia coli in which protein localization is altered. Utilizing this system, we identify proteins required for proper positioning of the Shigella autotransporter IcsA. Autotransporters, widely …

Burnout Among Chiropractic Practitioners: Real Or Imagined An Exploratory Study Protocol, Shawn Williams, Stanley Innes

Publications and Research

Burnout is a psychological syndrome of emotional exhaustion, depersonalization and reduced personal accomplishment that has been found to exist in a significant number of healthcare and helping professionals. It imposes a significant societal burden by shortened practitioner lifespan, decreased efficiency, negative health outcomes and poorer levels of patient care. Theoretical models suggest that it appears to be the result of a complex interaction between job resources and job demands. It may be reasonable to conclude that Chiropractic professionals experience similar vocational demands and thus experience significant levels of occupational stress and subsequent burnout. However the data on burnout within the …

Could Focused Deterrence Strategies Reduce Drug-Related Violence In Mexico?, Douglas N. Evans

Publications and Research

Drug-related killings in Mexico have reached frightening proportions. This databit summarizes recommendations by Professor Mark Kleiman and analyzes drug-related murders in the Federal States of Mexico compared to total murders in U.S jurisdictions of comparable size.

The Role Of Culture On The Leadership Styles Of Generation X Women In Nigeria, Remi Alapo

Publications and Research

No abstract provided.

Epidemiology And Management Of Depression Following Coronary Heart Disease Diagnosis In Women, Siqin Ye, Ellen-Ge Denton, Lauren T. Wasson, Karina W. Davidson

Publications and Research

Coronary heart disease (CHD) and depression are both highly prevalent in women. Importantly, depression is associated with significantly elevated morbidity and mortality in women with CHD. There are intriguing speculations about biological mechanisms underlying this association, such as endothelial dysfunction, subclinical atherosclerosis, inflammation, and autonomic dysregulation. Social and behavioral mechanisms, such as lack of social support and physical inactivity, have also been shown to play important roles. Unfortunately, many randomized clinical trials of counseling and pharmacologic interventions for depression in patients with CHD have failed to improve cardiovascular outcomes, and in fact have raised the possibility that interventions might be …

Independent Recruitment Of A Flavin-Dependent Monooxygenase For Safe Accumulation Of Sequestered Pyrrolizidine Alkaloids In Grasshoppers And Moths, Linzhu Wang, Till Beuerle, James Timbilla, Dietrich Ober

Publications and Research

Several insect lineages have developed diverse strategies to sequester toxic pyrrolizidine alkaloids from food-plants for their own defense. Here, we show that in two highly divergent insect taxa, the hemimetabolous grasshoppers and the holometabolous butterflies, an almost identical strategy evolved independently for safe accumulation of pyrrolizidine alkaloids. This strategy involves a pyrrolizidine alkaloid N-oxygenase that transfers the pyrrolizidine alkaloids to their respective N-oxide, enabling the insects to avoid high concentrations of toxic pyrrolizidine alkaloids in the hemolymph. We have identified a pyrrolizidine alkaloid N-oxygenase, which is a flavin-dependent monooxygenase, of the grasshopper Zonocerus variegatus. After heterologous expression in E. coli, …

Recent Increases In Drug Arrests Were For Possession, Not Sales, Jeffrey A. Butts

Publications and Research

Between 1991 and 2006, arrests for drug sales and manufacturing dropped while arrests for possession grew, with the largest growth seen in youth under 18. This databit looks at drug-related arrests for youth under 18, young adults 18 – 24, and adults 25 and older.

Sendai Virus-Based Liposomes Enable Targeted Cytosolic Delivery Of Nanoparticles In Brain Tumor-Derived Cells, Veronica Dudu, Veronica Rotari, Maribel Vazquez

Publications and Research

BACKGROUND: Nanotechnology-based bioassays that detect the presence and/or absence of a combination of cell markers are increasingly used to identify stem or progenitor cells, assess cell heterogeneity, and evaluate tumor malignancy and/or chemoresistance. Delivery methods that enable nanoparticles to rapidly detect emerging, intracellular markers within cell clusters of biopsies will greatly aid in tumor characterization, analysis of functional state and development of treatment regimens.

RESULTS: Experiments utilized the Sendai virus to achieve in vitro, cytosolic delivery of Quantum dots in cells cultured from Human brain tumors. Using fluorescence microscopy and Transmission Electron Microscopy, in vitro experiments illustrated that these virus-based …

Coupling Of Fog And Marine Microbial Content In The Near-Shore Coastal Environment, M. E. Ducker, Gregory D. O'Mullan, K. C. Weathers, A. R. Juhl, M. Uriarte

Publications and Research

Microbes in the atmosphere (microbial aerosols) play an important role in climate and provide an ecological and biogeochemical connection between oceanic, atmospheric, and terrestrial environments. However, the sources and environmental factors controlling the concentration, diversity, transport, and viability of microbial aerosols are poorly understood. This study examined culturable microbial aerosols from a coastal environment in Maine (USA) and determined the effect of onshore wind speed and fog presence on deposition rate, source, and community composition. During fog events with low onshore winds (

Genetic Signatures Of A Demographic Collapse In A Large-Bodied Forest Dwelling Primate (Mandrillus Leucophaeus), Nelson Ting, Christos Astaras, Gail Hearn, Shaya Honarvar, Joel Corush, Andrew S. Burrell, Naomi Phillips, Bethan J. Morgan, Elizabeth L. Gadsby, Ryan L. Raaum, Christian Roos

Publications and Research

It is difficult to predict how current climate change will affect wildlife species adapted to a tropical rainforest environment. Understanding how population dynamics fluctuated in such species throughout periods of past climatic change can provide insight into this issue. The drill (Mandrillus leucophaeus) is a large-bodied rainforest adapted mammal found in West Central Africa. In the middle of this endangered monkey’s geographic range is Lake Barombi Mbo, which has a well-documented palynological record of environmental change that dates to the Late Pleistocene. We used a Bayesian coalescent-based framework to analyze 2,076 base pairs of mitochondrial DNA across wild drill populations …

As Serious Juvenile Crime Declined, Police Made More Arrests For Less Serious Offenses, Jeffrey A. Butts

Publications and Research

Arrests by law enforcement for youth under 18 reached a 30-year low in 2012. Arrests for the eight serious offenses included in the FBI crime index decreased by almost half from 1995 to 2010. This databit looks at juvenile arrests in total and by offense from 1980 to 2010.