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De Novo Synthesis Of Phosphatidylcholine Is Essential For The Promastigote But Not Amastigote Stage In Leishmania Major, Samrat Moitra, Somrita Basu, Mattie Pawlowic, Fong-Fu Hsu, Kai Zhang

Open Access Publications

Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% of total lipids in

Neuroscience Of Object Relations In Health And Disorder: A Proposal For An Integrative Model, Dragan M Svrakic, Charles F Zorumski

Open Access Publications

Recent advances in the neuroscience of episodic memory provide a framework to integrate object relations theory, a psychoanalytic model of mind development, with potential neural mechanisms. Object relations are primordial cognitive-affective units of the mind derived from survival- and safety-level experiences with caretakers during phase-sensitive periods of infancy and toddlerhood. Because these are learning experiences, their neural substrate likely involves memory, here affect-enhanced episodic memory. Inaugural object relations are encoded by the hippocampus-amygdala synaptic plasticity, and systems-consolidated by medial prefrontal cortex (mPFC). Self- and object-mental representations, extracted from these early experiences, are at first dichotomized by contradictory affects evoked by …

Cerebrospinal Fluid Ncam-1 Concentration Is Associated With Neurodevelopmental Outcome In Post-Hemorrhagic Hydrocephalus Of Prematurity, David D. Limbrick, Diego M. Morales, Et Al.

Open Access Publications

OBJECTIVE: Efforts directed at mitigating neurological disability in preterm infants with intraventricular hemorrhage (IVH) and post hemorrhagic hydrocephalus (PHH) are limited by a dearth of quantifiable metrics capable of predicting long-term outcome. The objective of this study was to examine the relationships between candidate cerebrospinal fluid (CSF) biomarkers of PHH and neurodevelopmental outcomes in infants undergoing neurosurgical treatment for PHH.

STUDY DESIGN: Preterm infants with PHH were enrolled across the Hydrocephalus Clinical Research Network. CSF samples were collected at the time of temporizing neurosurgical procedure (n = 98). Amyloid precursor protein (APP), L1CAM, NCAM-1, and total protein (TP) were compared …

Transcriptome Analysis Of Dnmt3l Knock-Out Mice Derived Multipotent Mesenchymal Stem/Stromal Cells During Osteogenic Differentiation, Chih-Yi Yang, Rita Jui-Hsien Lu, Ming-Kang Lee, Felix Shih-Hsian Hsiao, Ya-Ping Yen, Chun-Chun Cheng, Pu-Sheng Hsu, Yi-Tzang Tsai, Shih-Kuo Chen, I-Hsuan Liu, Pao-Yang Chen, Shau-Ping Lin

Open Access Publications

Multipotent mesenchymal stem/stromal cells (MSCs) exhibit great potential for cell-based therapy. Proper epigenomic signatures in MSCs are important for the maintenance and the subsequent differentiation potential. The DNA methyltransferase 3-like (DNMT3L) that was mainly expressed in the embryonic stem (ES) cells and the developing germ cells plays an important role in shaping the epigenetic landscape. Here, we report the reduced colony forming ability and impaired

Gestational Age-Specific Complete Blood Count Signatures In Necrotizing Enterocolitis, Julia M Pantalone, Silvia Liu, Oluwabunmi O Olaloye, Erica C Prochaska, Toby Yanowitz, Melissa M Riley, Justin R Buland, Beverly S Brozanski, Misty Good, Liza Konnikova

Open Access Publications

No abstract provided.

Ontogenetic Oxycodone Exposure Affects Early Life Communicative Behaviors, Sensorimotor Reflexes, And Weight Trajectory In Mice, Elena Minakova, Simona Sarafinovska, Marwa O Mikati, Kia M Barclay, Katherine B Mccullough, Joseph D Dougherty, Ream Al-Hasani, Susan E Maloney

Open Access Publications

Nationwide, opioid misuse among pregnant women has risen four-fold from 1999 to 2014, with commensurate increase in neonates hospitalized for neonatal abstinence syndrome (NAS). NAS occurs when a fetus exposed to opioids

Srf Is Required For Maintenance Of Astrocytes In Non-Reactive State In The Mammalian Brain, Monika Jain, Soumen Das, Paul P Y Lu, Garima Virmani, Sumitha Soman, Surya Chandra Rao Thumu, David H Gutmann, Narendrakumar Ramanan

Open Access Publications

Astrocytes play several critical roles in the normal functioning of the mammalian brain, including ion homeostasis, synapse formation, and synaptic plasticity. Following injury and infection or in the setting of neurodegeneration, astrocytes become hypertrophic and reactive, a process termed astrogliosis. Although acute reactive gliosis is beneficial in limiting further tissue damage, chronic gliosis becomes detrimental for neuronal recovery and regeneration. Several extracellular factors have been identified that generate reactive astrocytes; however, very little is known about the cell-autonomous transcriptional mechanisms that regulate the maintenance of astrocytes in the normal non-reactive state. Here, we show that conditional deletion of the stimulus-dependent …

Brain Volumetric Deficits In Mapt Mutation Carriers: A Multisite Study, Stephanie A. Chu, Nupur Ghoshal, Et Al

Open Access Publications

No abstract provided.

High-Fat Diet Activates Liver Ipla2Γ Generating Eicosanoids That Mediate Metabolic Stress, Sung Ho Moon, Beverly Gibson Dilthey, Xinping Liu, Shaoping Guan, Harold F. Sims, Richard W. Gross

Open Access Publications

No abstract provided.

4Β-Hydroxycholesterol Is A Prolipogenic Factor That Promotes Srebp1c Expression And Activity Through The Liver X Receptor, Ofer Moldavski, Peter-James H. Zushin, Charles A. Berdan, Robert J. Van Eijkeren, Xuntian Jiang, Mingxing Qian, Daniel S. Ory, Douglas F. Covey, Daniel K. Nomura, Andreas Stahl, Ethan J. Weiss, Roberto Zoncu

Open Access Publications

No abstract provided.

Brd4-Bound Enhancers Drive Cell-Intrinsic Sex Differences In Glioblastoma, Najla Kfoury, Zongtai Qi, Briana C. Prager, Michael N. Wilkinson, Lauren Broestl, Kristopher C. Berrett, Arnav Moudgil, Sumithra Sankararaman, Xuhua Chen, Jason Gertz, Jeremy N. Rich, Robi D. Mitra, Joshua B. Rubin

Open Access Publications

No abstract provided.

Cisplatin +/− Rucaparib After Preoperative Chemotherapy In Patients With Triple-Negative Or Brca Mutated Breast Cancer, Maitri Kalra, Yang Tong, David R. Jones, Tom Walsh, Michael A. Danso, Cynthia X. Ma, Paula Silverman, Mary-Claire King, Sunil S. Badve, Susan M. Perkins, Kathy D. Miller

Open Access Publications

No abstract provided.

Dnmt3a Deficiency In The Skin Causes Focal, Canonical Dna Hypomethylation And A Cellular Proliferation Phenotype, David Y. Chen, Ian M. Ferguson, Krista A. Braun, Leslie A. Sutton, Nichole M. Helton, Sai Mukund Ramakrishnan, Amanda M. Smith, Christopher A. Miller, Timothy J. Ley

Open Access Publications

No abstract provided.

Calcium Carbonate Nanoparticles Stimulate Cancer Cell Reprogramming To Suppress Tumor Growth And Invasion In An Organ‑On‑A‑Chip System, Sandra F. Lam, Kevin W. Bishop, Rachel Mintz, Lei Fang, Samuel Achilefu

Open Access Publications

No abstract provided.

Single-Cell Analyses Of Crohn’S Disease Tissues Reveal Intestinal Intraepithelial T Cells Heterogeneity And Altered Subset Distributions, Natalia Jaeger, Ramya Gamini, Marina Cella, Jorge L. Schettini, Mattia Bugatti, Shanrong Zhao, Charles V. Rosadini, Ekaterina Esaulova, Blanda Di Luccia, Baylee Kinnett, William Vermi, Maxim N. Artyomov, Thomas A. Wynn, Ramnik J. Xavier, Scott A. Jelinsky, Marco Colonna

Open Access Publications

No abstract provided.

Old Vaccines For New Infections: Exploiting Innate Immunity To Control Covid-19 And Prevent Future Pandemics, Konstantin Chumakov, Michael S. Avidan, Christine S. Benn, Stefano M. Bertozzi, Lawrence Blatt, Angela Y. Chang, Dean T. Jamison, Shabaana A. Khader, Shyam Kottilil, Mihai G. Netea, Annie Sparrow, Robert C. Gallo

Open Access Publications

No abstract provided.

Role Of Sirt1 In Isoflurane Conditioning-Induced Neurovascular Protection Against Delayed Cerebral Ischemia Secondary To Subarachnoid Hemorrhage, Meizi Liu, Keshav Jayaraman, Tusar Giri, Gregory J Zipfel, Umeshkumar Athiraman

Open Access Publications

We recently reported that isoflurane conditioning provided multifaceted protection against subarachnoid hemorrhage (SAH)-induced delayed cerebral ischemia (DCI), and this protection was through the upregulation of endothelial nitric oxide synthase (eNOS). SIRT1, an NAD-dependent deacetylase, was shown to be one of the critical regulators of eNOS. The aim of our current study is to examine the role of SIRT1 in isoflurane conditioning-induced neurovascular protection against SAH-induced DCI. Mice were divided into four groups: sham, SAH, or SAH with isoflurane conditioning (with and without EX-527). Experimental SAH via endovascular perforation was performed. Anesthetic conditioning was performed with isoflurane 2% for 1 h, …

Gut Microbiome Contributions To Altered Metabolism In A Pig Model Of Undernutrition, Hao-Wei Chang, Nathan P. Mcnulty, Matthew C. Hibberd, David O'Donnell, Jiye Cheng, Vincent Lombard, Bernard Henrissat, Olga Ilkayeva, Michael J. Muehlbauer, Christopher B. Newgard, Michael J. Barratt, Xi Lin, Jack Odle, Jeffrey I. Gordon

Open Access Publications

No abstract provided.

A Domestic Cat Whole Exome Sequencing Resource For Trait Discovery, Alana R. Rodney, Rueben M. Buckley, Robert S. Fulton, Catrina Fronick, Todd Richmond, Christopher R. Helps, Peter Pantke, Dianne J. Trent, Karen M. Vernau, John S. Munday, Andrew C. Lewin, Rondo Middleton, Leslie A. Lyons, Wesley C. Warren

Open Access Publications

No abstract provided.

Tdp-43 And Pink1 Mediate Chchd10s59l Mutation–Induced Defects In Drosophila And In Vitro, Minwoo Baek, Yun-Jeong Choe, Sylvie Bannwarth, Jihye Kim, Swati Maitra, Gerald W. Dorn Ii, J. Paul Taylor, Veronique Paquis-Flucklinger, Nam Chul Kim

Open Access Publications

No abstract provided.

Experimental Methods To Study The Pathogenesis Of Human Enteric Rna Viruses, Somya Aggarwal, Ebrahim Hassan, Megan T Baldridge

Open Access Publications

Every year, millions of children are infected with viruses that target the gastrointestinal tract, causing acute gastroenteritis and diarrheal illness. Indeed, approximately 700 million episodes of diarrhea occur in children under five annually, with RNA viruses norovirus, rotavirus, and astrovirus serving as major causative pathogens. Numerous methodological advancements in recent years, including the establishment of novel cultivation systems using enteroids as well as the development of murine and other animal models of infection, have helped provide insight into many features of viral pathogenesis. However, many aspects of enteric viral infections remain elusive, demanding further study. Here, we describe the different …

Current And Prospective Methods For Assessing Anti-Tumor Immunity In Colorectal Cancer, Yulia I Nussbaum, Yariswamy Manjunath, Kanve N Suvilesh, Wesley C Warren, Chi-Ren Shyu, Jussuf T Kaifi, Matthew A Ciorba, Jonathan B Mitchem

Open Access Publications

Colorectal cancer (CRC) remains one of the deadliest malignancies worldwide despite recent progress in treatment strategies. Though immune checkpoint inhibition has proven effective for a number of other tumors, it offers benefits in only a small group of CRC patients with high microsatellite instability. In general, heterogenous cell groups in the tumor microenvironment are considered as the major barrier for unveiling the causes of low immune response. Therefore, deconvolution of cellular components in highly heterogeneous microenvironments is crucial for understanding the immune contexture of cancer. In this review, we assimilate current knowledge and recent studies examining anti-tumor immunity in CRC. …

Tim4 Expression By Dendritic Cells Mediates Uptake Of Tumor-Associated Antigens And Anti-Tumor Responses, Nicoletta Caronni, William Vermi, Et Al.

Open Access Publications

No abstract provided.

Surgical Infection Society 2020 Updated Guidelines On The Management Of Complicated Skin And Soft Tissue Infections, Therese M. Duane, Jared M. Huston, Morgan Collom, Adam Beyer, Sara Parli, Sara Buckman, Mark Shapiro, Amy Mcdonald, Jose Diaz, Jeffrey M. Tessier, James Sanders

Open Access Publications

No abstract provided.

Pre-Clinical Common Data Elements For Traumatic Brain Injury Research: Progress And Use Cases, Michelle C. Laplaca, Stuart H. Friess, Et Al.

Open Access Publications

No abstract provided.

Functional Interaction Between Wnt And Bmp Signaling In Periosteal Bone Growth, Deye Song, Guangxu He, Yu Shi, Jiangdong Ni, Fanxin Long

Open Access Publications

No abstract provided.

Interhemispheric Parietal-Frontal Connectivity Predicts The Ability To Acquire A Nondominant Hand Skill, Benjamin A. Philip, Mark P. Mcavoy, Scott H. Frey

Open Access Publications

No abstract provided.

Impact Of Intrauterine Fetal Resuscitation With Oxygen On Oxidative Stress In The Developing Rat Brain, Jia Jiang, Tusar Giri, Nandini Raghuraman, Alison G. Cahill, Arvind Palanisamy

Open Access Publications

No abstract provided.

Acetylated Tau Inhibits Chaperone-Mediated Autophagy And Promotes Tau Pathology Propagation In Mice, Benjamin Caballero, Celeste M. Karch, Et Al.

Open Access Publications

No abstract provided.

Modular Network Between Postrhinal Visual Cortex, Amygdala, And Entorhinal Cortex, Andrew M. Meier, Quanxin Wang, Weiqing Ji, Jehan Ganachaud, Andreas Burkhalter

Open Access Publications

The postrhinal area (POR) is a known center for integrating spatial with nonspatial visual information and a possible hub for influencing landmark navigation by affective input from the amygdala. This may involve specific circuits within muscarinic acetylcholine receptor 2 (M2)-positive (M2