Digital Commons Network^™

Phenomenological Study Of Adult Lived Experiences After The Loss Of A Parent And Its Effects On The Perception Of Those Adults, Victor Khaula

Master's Theses

Some things are never truly understood in life until you have the personal experience of living through them. This qualitative research was aimed at understanding the adult-lived experience after parental loss and how living through such an experience led those adults to change their perception about bereavement. The major question was “Why did the death of my mother communicate things to me that I never understood when I ministered to those who had lost their parents?” Experiencing the death of my mother was studied in relation to the lived experiences of three other Seventh-day Adventist adults from my university who …

Population Genetics And Distribution Of The Oriental Weatherfish, Misgurnus Anguillicaudatus, In Chicago Area Waterways, John Belcik

Master's Theses

Oriental Weatherfish (Misgurnus anguillicaudatus), native to Southeast Asia, are freshwater fish belonging to the family Cobitidae (loaches). They are benthic fish with the ability to breathe atmospheric air, and exhibit an unusual behavior of swimming vertically in the water column in response to changes in barometric pressure. Oriental Weatherfish appear to be successful invaders to North American waterways. Within the last century they have been sighted in freshwater systems throughout the United States, yet the distribution and source of this invasion are largely unknown. This study investigates the distribution patterns, surveying methods, and population genetics of the Oriental Weatherfish within …

The Art Of The Resistance: Participation In The Slipstream And Acts Of Resistance In A Culinary Re-Entry Program, Anna R. Wilcoxson

Master's Theses

Social policies since 1996 require that low income people participate in job training programs in order to receive social benefits under the “New Welfare State.” Many scholars have argued that job training programs aim to produce docile workers, who carry out only highly routinized work where little discretion is needed. Through ethnographic observation and interviews, I identify three means by which trainees manage the dual expectations of docility and the creativity demanded in a kitchen setting. First, they operate in a routine fashion, as if in a slipstream; second, they bank confidence by disregarding rules because of skill or favor …

Undergraduates' Understanding Of Sexual Consent, Melissa Ann Kinsella

Master's Theses

In 2014, the White House launched its public awareness campaign, It's On Us, to end sexual assault on college campuses. A large portion of the initiative implemented on college campuses has been dedicated to the issue of sexual consent. However, as past research has illustrated, consent is a complex issue (Beres, Herold, and Maitland 2004; Beres 2007; Humphreys 2007; Jozkowski and Peterson 2013; and Jozkowski, Peterson, Sanders, Dennis, and Reece 2014). It is one that nonetheless can and should be studied. Face-to-face, semi-structured interviews with self-selected sexually active undergraduate students were conducted in an attempt to untangle how students are …

Koinonia: Experiences Of Cultural Diversity In The Andrews University Theological Seminary, Joshua N. Brantley

Master's Theses

The Andrews University Theological Seminary has a long standing history of attracting students from around the world seeking to acquire a Masters of Divinity (M.Div.). However, it is unclear how students of various backgrounds experience the seminary’s diversity. This study conducted an online survey with M.Div. students to examine the seminary’s performance according to the respondents as a whole and based on different ethnicities. The first hypothesis was that students of various ethnicities experienced the incorporation of diversity into the curriculum differently. The second hypothesis was that seminary events did not reflect the diversity of the student body and were …

Lip Detection And Adaptive Tracking, Benjamin Wang

Master's Theses

Performance of automatic speech recognition (ASR) systems utilizing only acoustic information degrades significantly in noisy environments such as a car cabins. Incorporating audio and visual information together can improve performance in these situations. This work proposes a lip detection and tracking algorithm to serve as a visual front end to an audio-visual automatic speech recognition (AVASR) system.

Several color spaces are examined that are effective for segmenting lips from skin pixels. These color components and several features are used to characterize lips and to train cascaded lip detectors. Pre- and post-processing techniques are employed to maximize detector accuracy. The trained …

Unravelling The Paleotopography And Extent Of Eocene-Oligocene Gravel Deposition By Ancient Sierran Drainage Systems, Christina Marie Tipp

Master's Theses

The Tertiary gravels deposited along the western flank of the Sierra Nevada mountains elucidate the nature of the paleochannels and paleotopography of the range in the Eocene-Oligocene epochs. Numerous geologic maps and studies were compiled to place existing Tertiary gravels onto topographic maps and cross-sections extracted from ArcGIS. Based on the remnant deposits, the extent of deposition of the gravels during the Eocene-Oligocene epoch was inferred, and average end-area values were measured from the cross-sections to calculate the volume of the Tertiary gravels that was originally deposited. The map of the inferred deposits was also used to measure surface gradients …

Aquatic Insect Community Structure And Secondary Production In Coastal Wetland Ponds Of The Copper River Delta, Alaska: Influence Of Water Temperature And Macrophyte Community Structure, Chantel Caldwell

Master's Theses

The Copper River Delta (CRD), southcentral Alaska, is one of the world’s largest continuous coastal wetlands and is largely composed of sloughs, lakes, and ponds. Due to coastal topography the east side of the Copper River (East Delta) is disproportionately impacted by a cold continental air mass. Wetland communities of the CRD were also impacted by a 9.2 magnitude earthquake in 1964 that shifted the more coastal portion of the CRD from tidally influenced ponds to freshwater ponds.

The West-East temperature gradient across the CRD coupled with landscape type (uplifted marsh (UM) and outwash plain (OP)) creates four regions (West-UM, …

Thrown To The Wolves: The Obstacles And Barriers Of International Student Athletes Prior To And During Enrollment In American Universities, Mary Elizabeth Boucher

Master's Theses

International student athletes (ISA’s) play an integral role among our nations university system. They diversify our colleges, provide our American students a chance to become familiar with other cultures, and they add intense value to our collegiate athletic programs. In order to maintain this diversity in our nation’s universities, we need to further understand the experiences of these student-athletes. It is inherent to provide international student-athletes with positive experiences as to fuel the globalization of sport.

This project is a small pilot study which focuses on male and female swimmers from Division I universities. It provides a closer look at …

The Effects Of Superordinate Identity Recategorization And Social Value Orientation On Ethical Decision-Making In A Business Dilemma, Thomas Steven Colville

Master's Theses

The purpose of the present study was to investigate potential solutions to the prevalence of unethical behavior in groups, specifically through the use of superordinate recategorization. Superordinate recategorization allows group members to feel an affinity with others not typically included in their in-group. This should promote ethical decision-making by enabling the inherent in-group favoring tendencies found in groups to be extended to a more inclusive category. In the context of the present study, the ethicality of participants' behavior in the Panalba scenario was determined by their choices on a 1 to 6 scale from least to most ethical. Additionally, participants’ …

An Investigation Into Discrimination: Racially Identifiable Names And The Effects They Have On The Home Renting Process, Francis Thomas Flynn

Master's Theses

Many urban sociologists do not adequately address ingrained systemic forms of racism

that exist in society today, such as the effects that racially identifiable names representing the larger idea of racial bias have on different social processes. This paper investigates racial housing discrimination in Chicago through analyzing the affect that racially identifiable names have on the home renting process. I conducted a field experiment in which I inquired about the availability of 96 properties throughout various locations in Chicago. Specifically, I created four email addresses linked to four racially identifiable names and sent the exact same fictitious email script from …

Privatizing Laws: Examining The Relationship Between Privatization Elements In State Education Laws And Fourth Grade Academic Performance, Stephanie Romeo

Master's Theses

In the midst of a global context tied to expanding market connections, the United States adopted privatized education reform that has only been increasing over the years. Specific states have adopted privatization in varying degrees and forms, including vouchers, charter schools, public-private partnerships, contracting out services, and virtual education. States have implemented privatization forms for different reasons, such as to improve student achievement, save money, or provide for increased student choice. This research study evaluates the impact of privatization reforms on student achievement by examining the relationship between various privatization elements in state laws and student achievement. To do so, …

Getting Students Employed: 21st Century Learning Competences And Career Competences, Kelly Cebold Sundberg

Master's Theses

This thesis explores the nexus between education and the economy in the 21st century knowledge-based economy to understand the relationship between the learning competences – as described in 21st century frameworks – and career competences – as recommended in the job descriptions of available employment opportunities. Theoretically, this study is grounded in human capital theory and it explores the indiscriminate nature of the data regarding a student’s required education level to achieve the prescribed learning competences. With the aid of a quantitative content analysis of selected employment opportunities, the research sought to explore: How are learning competences, as outlined by …

The Effects Of Parental Functioning And Socioeconomic Status On Initial Child Psychopathology Symptoms And Treatment Outcomes Following A Brief Trauma Focused Cognitive Behavioral Therapy Group, Anna Ros Ros

Master's Theses

The present study will investigate the impact of parental psychopathology and socioeconomic status on the severity of child PTSD and depression symptoms at baseline, in addition to the impact on treatment effectiveness. First, the study will examine how the presence of parental PTSD, depression and hostility act as proximal risk factors for the baseline severity of child PTSD and depression symptoms. Further, the study will investigate how these proximal parental factors affect symptom reduction following intervention for these children. Additionally, the current study will examine how low socioeconomic status affects baseline severity of child PTSD and depression symptoms as well …

Using Tcr Transgenic, Gp100 Reactive T Cells And Checkpoint Inhibition To Target Lymphangioleimyomatosis, Christian Ankney

Master's Theses

Lymphangioleiomyomatosis (LAM) is a low-grade neoplastic disease affecting primarily women. It is characterized by cystic lung disease as well as renal and retroperitoneal tumors called angiomyolipomas and lymphangiomas. Tumor cells have smooth muscle features as well as neuroendocrine cell surface markers, and the disease can be diagnosed by HMB45 staining of tumor cells. We questioned whether expression of melanocytic antigens, specifically gp100, creates an opportunity to treat LAM by adoptive T cell transfer. LAM lung lesions demonstrate poor immune surveillance, therefore adoptive T cell transfer could offer benefits in this disease.

A link was made between melanoma and depigmenting vitiligo …

Utilizing Single-Molecule Fret Methods To Study Conformational Changes In Trim5Α, Margret Suzanne Bradley

Master's Theses

Single-molecule FRET (smFRET) is a method by which dynamic conformational changes can be monitored in a protein microscopically and in real time. smFRET relies on the creation of FRET (Förster Resonance Energy Transfer) between small molecule fluorophores conjugated to the biomolecules of interest. FRET efficiency allows calculation of interfluorophore distances. Changes in FRET efficiency represent changes in protein conformation which can inform further structural and molecular studies of the protein of interest. For example, in the Campbell Lab, we study the protein TRIM5α, an antiretroviral cellular protein which can cause premature dissociation of the HIV capsid core by an unknown …

The Devil's In The Emails: A Sociological Examination Of Organizational Failure, William Howard Burr

Master's Theses

It is often argued that the market, with its "invisible hand," displays an inherent bent towards maximizing utility and delivering the "greatest good to the greatest number." Faith in the market to act as benevolent overlord is not only misguided but, as revealed during the Great Recession, a fantasy. Analyzing emails made public following the Lehman Brothers bankruptcy, this paper considers the organizational culture within Lehman Brothers leading up to history's largest bankruptcy in order to emphasize the role of interaction within an outcome otherwise uncritically categorized as the unavoidable product of market fluctuations. Demonstrating how Lehman employees adopted the …

Creating A Bret Assay To Monitor The Interaction Between Β-Arrestin-1 And Stam-1, James Buhrmaster

Master's Theses

CXCR4 is a chemokine receptor that is overexpressed in multiple disease states, including cancer. Understanding the mechanisms by which cells regulate CXCR4 expression is of high importance, as they can reveal downstream effectors that can potentially be targeted pharmacologically to more effectively treat diseases with fewer side effects.

CXCR4 is internalized in response to stimulation by its ligand CXCL12, and localizes to early endosomes as part of a homologous desensitization mechanism. From the endosome, CXCR4 can enter one of two pathways whereby it is either recycled back to the plasma membrane, where it can undergo another signaling event, or it …

The Unconventional Secretion Of Alpha-Synuclein By Autophagic Mechanisms And The Novel Characterization Of Pathological Alpha-Synuclein Associated Extracellular Vesicles, Kevin Burbidge

Master's Theses

Synucleinopathies are a group of neurodegenerative diseases associated with the intracellular accumulation of aggregated α-synuclein, a neurotoxic process resulting in cell death. Research indicates that pathology results from α-synuclein misfolding and self-propagation, as well as, cell-to-cell transmission. Furthermore, pathological α-synuclein transmission may occur through unconventional secretion.

In this thesis, we investigated how autophagy influences unconventional secretion of our novel α-synuclein dual-split protein model that we first verified by reproducing documented results from treatment of known autophagic regulators. Afterwards, we investigated how knocking out autophagic proteins ATG7 and Beclin-1 influenced α-synuclein secretion.

Additionally, we developed a methodology for analyzing extracellular vesicles …

Nutrient Removal And Vegetation Recovery Through Successive Harvesting Of The Invasive Hybrid Cattail (Typha X Glauca) In Great Lakes Coastal Wetlands, Kelsey Berke

Master's Theses

Excess nutrient levels have led to invasive hybrid cattail (Typha × glauca) dominance in many Laurentian Great Lakes coastal wetlands. Its ability to alter wetland structure and function has made T. × glauca a target invasive species with respect to Great Lakes coastal wetland

restoration. This study investigated the effect of harvesting invasive T. × glauca biomass on nutrient removal, specifically nitrogen and phosphorus, and plant diversity recovery in Great Lakes coastal wetland ecosystems. This study demonstrated that without considering external nutrient inputs and outputs, a single harvest at Cheboygan Marsh, MI could remove ~2% of the total N and …

The Impact Of Chronic Stress On Childhood Obesity And The Protective Effects Of Parental Warmth, Laura M. L. Distel

Master's Theses

Mexican American children have the highest rates of obesity in the U.S. Mexican American children may experience chronic stress, which has been linked to child obesity. Hair cortisol accumulation has been implicated as a mechanism for this association. Highly sensitive parenting may buffer the harmful effects of chronic stress and hair cortisol on obesity. Thus, the aims of this project were to 1) identify the direct and indirect effects of chronic stress and hair cortisol on children's zBMI and 2) examine the protective effects of parental warmth. This study examined hair cortisol levels, zBMI and chronic stress of children ages …

Coping With Exposure To Community Violence: Assessing The Role Of Avoidance In Reducing Delinquency And Aggression In Low-Income Urban Adolescents, Cara Marie Diclemente

Master's Theses

Exposure to community violence has disabling effects on the mental health of youth in the US, with high rates of exposure for African American adolescents from underserved, urban communities. Past literature suggests that avoidant coping, specifically behavioral avoidance, may be most useful for youth exposed to uncontrollable stress. The current study assessed the utility of coping strategies in reducing aggression and delinquency in 267 sixth through eighth grade African American youth. First, confirmatory factor analyses revealed a three-factor structure of coping: cognitive approach, behavioral approach, and avoidance. Next, moderated moderation findings showed that for boys, avoidance and approach strategies increased …

The Effects Of Treating Multispecies Biofilms With Bacteriophage, Alexandria Francis Cooper

Master's Theses

Biofilms can be found in any environment within proximity to water and are problematic in an assortment of industries. Numerous efforts have been employed to dislodge biofilms including bacteriophages (viruses that infect bacteria). Phage remediation is a promising solution for combatting biofilms that form on catheters in long term use patients. These biofilms often result in catheter-associated urinary tract infections (CAUTIs) and are the most common type of healthcare related infection reported. Additionally, they result in longer hospital stays and increased morbidity. To date, most of the research on the topic focuses on single species biofilms, despite their rarity in …

Phenotyping Temperature-Sensitive Coronaviruses, Amani Eddins

Master's Theses

Coronaviruses (CoVs) can cause a range of symptoms; from a mild common cold to life threatening acute respiratory distress syndrome (ARDS) upon infection. These emerging viruses have the ability to be highly pathogenic and detrimental to the human population. Two prime examples of CoV emergence, SARS-CoV (Severe Acute Respiratory Syndrome) and MERS-CoV, (Middle Eastern Respiratory Syndrome), which exhibits the pandemic potential of emerging CoVs that gain human tropism. Given the increasing potential of an emerging CoV outbreak there is a state of urgency to develop vaccines that will help protect the human population against current and future circulating strains. The …

Utilizing Genetic Techniques To Identify Amino Acids Within The Putative Glycosyltransferase Sypq That Are Essential For Its Role In Biofilm Formation By Vibrio Fischeri, Mary Kathryn Flaherty

Master's Theses

The polysaccharide component of the V. fischeri biofilm is produced by proteins encoded by the symbiosis polysaccharide (syp) locus. The syp locus encodes 18 genes, six of which are putative glycosyltransferases (GTs). One of these GTs, sypQ, encodes a putative family 2 glycosyltransferase (GT-2). The mechanism by which SypQ facilitates biofilm formation is not yet understood. To uncover which specific residues are essential for the function of SypQ, I utilized Bioinformatics, site-directed mutagenesis, and random mutagenesis. The Bioinformatics identified putative GT-2 motifs in the SypQ primary sequence. The mutagenesis (both site-directed and random), identified five residues (D95, D151, S153, D236, …

Impact Of Visitation With Incarcerated Fathers On Behavioral Adjustment Among Children In The Foster Care System, Lauren Ashley Hindt

Master's Theses

This study sought to examine whether in-person visitation with incarcerated fathers related to less behavioral problems among children in foster care. The sample consisted of 282 youth (M = 10.18, SD = 2.36 years). Data were collected from the Illinois Department of Children and Family Services. Hierarchical Generalized Linear Modeling revealed paternal incarceration was associated with increased externalizing slope trajectories (ß1515 = .18, p = .025), but not internalizing. African American youth had lower externalizing slope trajectories compared to the remainder of the sample (ß20 = -.14, p = .032). The association between paternal incarceration and externalizing was attenuated …

Potential Efficacy Of Targeting Mll1 In Breast Cancer, Austin Gable Holmes

Master's Theses

In 2017, it is estimated that breast cancer will be the most prevalent newly diagnosed cancer in females, at 30% in the U.S. alone1. From the early 1990s to 2014 the death rate in females from breast cancer has dropped by 10% but still stands as the second highest cancer related death in females in the U.S.2. One of the biggest hurdles in breast cancer research is disease heterogeneity. New mechanisms of disease development and progression are encountered frequently. One mechanism studied in the past decades is epigenetics. It has been theorized that the cancer epigenome can maintain an abnormal …

The Novel Characterization Of Extracellular Vesicles Containing Proteins Which Have Been Implicated In The Pathogenesis Of Hiv Associated Neurocognitive Disorders, Grace Ispas

Master's Theses

Although Anti-Retroviral Therapy (ART) is practiced, HIV-1 positive individuals still experience HIV-Associated Neurocognitive Disorders (HAND), collectively described as the presentation of neurocognitive, behavioral and motor dysfunctions that decrease the quality of life, while increasing the mortality in ART suppressed HIV-1 positive patients.

Current literature suggests that extracellular vesicles are involved in the pathogenesis of HAND as they are believed to be transferring HIV-1 proteins to nearby neuronal cells. Although most studies assume homogeneity among populations, characterizing cellular proteins or RNA levels in bulk, we hypothesize that distinct populations of extracellular vesicles are released and that environmental conditions, including viral infections, …

Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen

Master's Theses

Clean catch was developed before the advancement of more sensitive assays for culturing urinary bacteria. I reassessed clean catch, endeavoring to optimize this method. Periurethral swabs and voided urines were routinely collected, while analyzing different aspects of the method. I determined that midstream urine contained the least amount of vulvo-vaginal contamination and that the time of collection did not impact the results. I also determined that the use of antiseptic wipes prior to void increased the level of vulvo-vaginal contamination. A small cohort of females collected daily urines over a 17-day period and kept a strict alcohol diary. Females who …

Fear Of Missing Out: A Moderated Mediation Approach To Social Media Use, Brynn M. Huguenel

Master's Theses

This study explores whether fear of missing out (FOMO) mediates the relation between social networking site (SNS) use and negative mental health outcomes, with moderators of Facebook activities and individual-level characteristics included. Short-term longitudinal data was collected from undergraduate students (N=296). Mediation analyses indicated that intensity of Facebook use did not predict anxiety or depression, as mediated by FOMO. Further, Facebook activities did not moderate the relation between intensity of Facebook use and FOMO, and social comparison and social connectedness did not moderate the relations between FOMO and negative mental health outcomes. Post-hoc analyses showed that social connectedness moderated the …