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Chironomidae (Diptera) Community Structure In Lakes Of Contrasting Morphometry, Landscape Position, And Water Chemistry, Conrad Stanley Zack

Master's Theses

Chironomidae (Diptera) surface-floating pupal exuviae were collected monthly during the ice-free season in 2010-2011 from six lakes in the North Temperate Lakes Long-Term Ecological Research site in northern Wisconsin. The goal of this study was to determine whether chironomid community structure reflected differences in lake morphometry (i.e., depth, area, shoreline development, etc.), water chemistry, and/or landscape position (i.e., elevation). Forty-six genera were identified from four subfamilies: Chironominae (57%), Orthocladiinae (28%), Tanypodinae (11%), and Diamesinae (4%). Tanytarsus, Ablabesmyia, and Psectrocladius were found in all six study lakes; whereas certain genera, such as Omisus, Protanypus, and Epoicocladius were each observed in only …

Characterizing Immune Response To Hiv-1 Infection In Bicd2-Knockout Cells, Omar Abdel-Rahim

Master's Theses

An important part of the HIV-1 infection cycle is the attachment of the intracellular viral core to the host microtubule network, facilitated by attachment of the viral capsid to cargo adaptor proteins. One such cargo adaptor is Bicaudal D Homolog Protein 2 (BICD2). BICD2 can attach to both the HIV-1 capsid and the dynein/dynactin complex and facilitate the trafficking of the viral core towards the host nucleus. Removal of BICD2 can disrupt this viral translocation, resulting in an elevated immune response that impairs productive HIV-1 infection. In my research, we investigated what viral particles are detected in the absence of …

Tejidos Urbanos: Representaciones Literarias Y Visuales De La Memoria En La Ciudad Imaginada, Marina Marisela Alvarez

Master's Theses

The urban imaginary is that which bases itself in a real physical and concrete space,

but is a construction created through the lense of a city's inhabitants. This thesis follows

narratives that have reconfigured the cities of México City, San Juan and Caracas from the

novels Hotel DF (2010), Simone (2011) and the film Pelo Malo (2013) respectively. These

contemporary Latin American & Caribbean narratives provide insight into sentiments

about one's country. Néstor García Canclini and Henri Lefebvre have argued the city is a

whole, and therefore it is analyzed as a microcosm and epicenter of its nation. With

emphasis …

Investigating Autophagy, Extracellular Vesicles, And Glycobiology, Benjamin Cook

Master's Theses

Autophagy is a cellular process of degradation which degrades cellular materials in acidic organelles called lysosomes. Impairment of lysosomal degradation can lead to lysosomal storage disorders such as Parkinson's Disease. In Parkinson's Disease neurotoxic α-synuclein can spread from cell-to-cell via extracellular vesicles. We investigated the changes in extra vesicular glycans upon perturbation of autophagy using a class of glycan binding molecules termed lectins.

We found that upon induction of autophagy causes EV glycans to increase while others remained consistent. Additionally, we found that specific glycans are increased more than others. Conversely, when lysosomal degradation was impaired, we observed a decrease …

Induction Of Zika Virus-Specific Cd8+ T Cells By Means Of Dna Vaccine, Frank Gambino Jr.

Master's Theses

Zika virus (ZIKV) is a member of the virus family flaviviridae and is transmitted via Aedes aegypti mosquitos. Monoclonal antibodies against dengue virus have been found to cross-react with ZIKV but show no ability to neutralize it. Further studies have shown that Stat2-/- mice given plasma from dengue positive donors exhibit a heightened disease phenotype when challenged with ZIKV. Antibody dependent enhancement is theorized to cause such effect. The goal of this study was to design a plasmid which, when encoded, creates an antigen that will be used to stimulate exclusively CD8+ T cells. The gene sequence of NS3 was …

Assessing English Language Learners' Motivation To Participate In Classroom Discussion, Holly Griskell

Master's Theses

This study assesses sixth-grade Spanish-speaking English Language Learners' (ELLs'; m age = 12.15 years old) participation and motivation to participate in classroom discussion through developing a 20-item measure, the Motivation for Classroom Discussion Questionnaire (MCD-Q) (Study 1 n = 258). We examined the relation between ELLs' bi-literacy and MCD-Q scores as well as amount of talk during discussion, measured by audio-recordings of their English Language Arts class (Study 2 n = 149). Study 1 findings indicated that the MCD-Q items cohered into five motivational constructs (value, language-efficacy, extrinsic motivation, social motivation, and interest). Study 2 findings showed the MCD-Q's predictive …

Understanding The Dynamics Of Protein Lipoylation In Staphylococcus Aureus, Sarah C. Flury

Master's Theses

Staphylococcus aureus is a commensal bacterium that also acts as an opportunistic pathogen. The pathogenicity of S. aureus has often been attributed to the wide range of virulence factors that the bacterium produces. While virulence factors do contribute a great deal, there is a growing field of research that aims to investigate the role of metabolism in bacterial virulence.

My project focuses on the necessity of a metabolic cofactor, lipoic acid. To ensure sufficient amounts of lipoic acid are available for enzyme activity, S. aureus has evolved two pathways to obtain the important nutrient. The lipoic acid salvage pathway is …

Cell Intrinsic Characteristics Of Cord Blood Naive Cd4 T Cells, Taylor Keller

Master's Theses

The neonatal immune system is functionally distinct from the adult immune system. Neonatal immune responses are less reactive than their adult counterparts, and as such, have an increased susceptibility to infection, resulting in increased duration and severity of disease. There is evidence that T-cells are partially responsible for the observed immunological differences between neonates and adults. Murine studies on neonatal immunity suggest that Th2 like responses predominate the neonatal immune system, an observation not found in the adult immune system. However, studies on human cord blood revealed that human neonates do not exhibit elevated Th2 like cytokines (with the exception …

Inhibition Of Mutant Egfr In Nsclc Promotes Endothelin-1-Mediated Nsclc Disease Progression, Stephen Ollosi

Master's Theses

Angiogenesis in NSCLC has been identified as important therapeutic target in combination with EGFR TKIs. However, only small incremental advancements have been made for the use of angiogenesis inhibitors in NSCLC and it remains elusive why the inhibition of VEGF-mediated neovascularization is not therapeutically efficacious. I present experimental evidence that a subpopulation of NSCLC cells with EGFR TKI-induced EMT contributes toward the attenuation of the response to EGFR TKI therapy. One of the hallmarks of cancer is heterogeneity and I have previously demonstrated that tumor heterogeneity within NSCLC cells lines harboring EGFR kinase domain mutations gives rise to divergent resistance …

Mechanistic Investigations Of The Iron(Iii)-Catalyzed Carbonyl-Olefin Metathesis Reaction, Susan Phan

Master's Theses

Iron(III)-catalyzed carbonyl-olefin ring-closing metathesis represents a new approach toward the assembly of molecules traditionally generated by olefinˆ’olefin metathesis or olefination. Herein, we report detailed synthetic, spectroscopic, kinetic, and computational studies to determine the mechanistic features imparted by iron(III), substrate, and temperature to the catalytic cycle. These data are consistent with an iron(III)-mediated asynchronous, concerted [2+2]-cycloaddition to form an intermediate oxetane as the turnover-limiting step. Fragmentation of the oxetane via Lewis acid-activation results in the formation of five- and six-membered unsaturated carbocycles.

Culturally Relevant Choral Music Pedagogy And Student Feedback, Theoni Richardson

Master's Theses

The purpose of this teacher action research study was to assess choral education pedagogy through the perspective of high school choir students. The research question asked: How can I utilize student interpretation and critique in order to empirically self-reflect on my choral teaching practice and its cultural relevance? Interpretation and critique are defined as student opportunities to give feedback, both positive and negative, in the form of qualitative oral discussion and quantitative, linear ranking. While research has shown that culturally relevant pedagogy allows for more student engagement and success, I hypothesized that this study would show that students who perceive …

Cryptic Diversification Of Two Widespread Species In Madagascar, Lynika Sharlice Strozier

Master's Theses

Using phylogenetic and phylogeographic tools to uncover hidden diversity within the genus Newtonia in Madagascar

Madagascar is known for its rich biodiversity and high level of endemic species that are found nowhere else. Cryptic diversification, defined as genetically and evolutionarily distinct species that are hard to detect because they are morphologically indistinguishable from their closest relatives, has been hypothesized to occur in many groups on Madagascar. Currently it is unclear to what extent this phenomenon occurs in birds because only a few studies have been conducted. My study examined the phylogenetic and phylogeographic patterns within a songbird genus, Newtonia, that …

An Exploratory Study Of Factors Influencing The Success Of Refugee Youth In College And University, Lea Tienou-Gustafson

Master's Theses

The educational needs, challenges and outcomes of refugee youth in the United States have been studied a great deal, particularly in regard to primary and secondary education. There is a dearth of research, however, on the refugee experience in higher education in the United States.

This study seeks to add to the body of literature on refugee education by exploring shared features of the refugee experience in higher education. Through an in-depth study of refugee youth in Chicago, the study seeks to understand their experiences before, while entering and during college and university, particularly how these experiences are tied to …

The Power Of A Stereotype: American Depictions Of The Black Woman In Film Media, Brittany Terry

Master's Theses

How are black women depicted in popular films? The significance of this study is that it sheds new light on the ways in which black women are depicted in film, and exemplifies some means to deconstruct dehumanizing representations of ourselves. This work advances the goal of institutionalizing more accurate visual accounts of black femaleness thereby exposing the inaccuracies of the dominant gaze. This study also transparently marks my intersectional positionality as a black feminist spectator-- simultaneously privileged and marginalized. I identify as a heterosexual woman of color, raised in an upper-middle class American community. As such, I benefit from classism …

International Student Intersectionality In The Trump Era, Sarah Tolman

Master's Theses

This study examines the impact of the current U.S. presidential administration's policies and rhetoric on international student experiences and identities. The researcher approaches this question through the theoretical lens of intersectionality, with the goal of highlighting the complexity and diversity of international student identities in this unique historical moment. Through in-depth narratives collected from interviews with 8 diverse international students, it becomes clear that the 2016 presidential election and the administration's rhetoric and policies have influenced international student experiences and identities in significant ways. Students perceive the statements and actions of the current government to have shifted their perspectives and …

Desde La Caballería A La Cristiandad: La Transformación Espiritual De Don Quijote, Amanda Kathleen Verduin

Master's Theses

Este presente trabajo es un análisis literario, histórico y teológico del protagonista de la obra famosa de Miguel de Cervantes Saavedra, El Ingenioso Hidalgo Don Quijote de la Mancha. Esta tesina presenta el argumento que Alonso Quijano experimenta una transformación espiritual significativa, desde el momento en que pierde el juicio y se reinventa en el caballero andante, don Quijote, hasta el momento en que muere en su lecho, alabando a Dios por su misericordia. Específicamente, asevero que esta transformación parece a la transformación que Jesucristo describió como el vínculo que se establece entre Dios y la persona que vuelve …

The Characterization Of Rna Content And Biogenesis Pathways Of Extracellular Vesicles That Have Been Implicated In The Pathogenesis Of Hiv-1 Associated Neurocognitive Disorders, Virginia Elizabeth Zwikelmaier

Master's Theses

Patients infected with HIV-1 adhering to Anti-Retroviral Therapy (ART) still suffer from a variety of neurocognitive, behavioral, and motor dysfunctions, collectively referred to has HIV-1 Associated Neurocognitive Disorders (HAND). Current research suggests the manifestations of HAND might in part be due to the dissemination of toxic HIV-1 components throughout the brain via extracellular vesicles (EVs). Our goal is to characterize these pathogenic EVs to begin to gain a better understanding of this EV-mediated pathology.

We took the first steps in developing a technique to assess the microRNA content of individual EVs but it needs to be heavily optimized to eliminate …

Lidocaine Attenuates An Induced Inflammatory Response, Stephanie Zack

Master's Theses

Inflammation is common to the pathogenesis of a variety of diseases. Therapeutics which can regulate and reduce damaging inflammation are therefore valuable in the treatment of inflammation and inflammatory diseases.

One commonly used analgesic, recently identified as limiting inflammation, is lidocaine. Additionally, alternative, less toxic therapeutics like the snake venom peptides, Zep 3 and Zep 4, are thought to have anti-inflammatory effects. Yet a well-defined mechanism or cellular target for lidocaine or Zep peptides' anti-inflammatory effects has not been proposed.

We aimed to determine whether lidocaine and Zep peptides reduce the release of the proinflammatory cytokine, IL-1β, from activated human …

Nonlinear Stiffness And Viscoelasticity Of Inhibitor-Treated Blood Clots By Tensile Testing, Wilson S. Eng

Master's Theses

Although blood clots are vital to wound healing, little is known about what factors influence clot stiffness and dynamic response. This work investigates the mechanics of inhibitor-treated clots by direct tensile testing using a custom designed system for forces below 1 N. Inhibitors that affect clot formation include blebbistatin, which affects myosin II movement on actin, and cytochalasin D, which affects actin polymerization. The hypothesis of this investigation is that blebbistatin will have a greater effect on mechanical behavior than cytochalasin D, because the inhibition of myosin II will weaken the overall clot more than actin. This hypothesis was investigated …

A Modular Multi-Level Converter For Energy Management Of Hybrid Energy-Storage Systems In Electric Vehicles, Sharon Sanjeev George

Master's Theses

Electric vehicles (EVs) are substantial applications of clean energy. Their effectiveness for mainstream transportation is predicated on the efficient use of stored energy within the vehicles’ power pack. Among rechargeable storage solutions, lithium-ion (Li-ion) battery cells have high energy density making them suitable to supply the EVs’ average power. However, the peak power requirements of the vehicles exert stress on the Li-ion cells due to their low pulsating power capabilities. Ultracapacitors can be used instead as the power-pulsating storage elements given their superior power density. Incorporating the two cell types for energy storage signifies a hybrid configuration that leads to …

The Diablo Winds Of Northern California: Climatology And Numerical Simulations, Carrie Lynn Bowers

Master's Theses

Extreme fire behavior in the San Francisco Bay Area (SFBA) has historically been associated with strong offshore wind events referred to locally as Diablo winds. A 17 year surface-based climatological analysis was performed to establish a definition of Diablo winds and to identify their frequency and spatial distribution. Synoptic composites of events were constructed using North American Regional Reanalysis, and high resolution Weather Research and Forecasting (WRF) model simulations were used to investigate the mesoscale dynamics of three significant Diablo wind events. Diablo winds were defined as dry northeasterly, downslope winds that occur in the SFBA with minimum sustained wind …

Vehicle Tracking Based On Historical Intersection Over Union, Shuai Hua

Master's Theses

Multi-object tracking (MOT) could be applied to many video analysis scenarios, such as vehicle speed estimation, vehicle re-identification, and vehicle abnormal behavior detection. A tracking task can be formulated as a data association problem, for which there exist many different types of solutions. Track-by-detection is one of the most common approaches for the MOT task. In this paradigm, the tracking algorithm relies on the detection results to decide whether detected vehicles in sequential frames belong to the same track. In our work, we developed a reliable vehicle tracker following this paradigm, while considering the balance between tracking efficiency and tracking …

Change And Transformation: The Harp As A Symbol Of Liminality In Tchaikovsky’S The Nutcracker (1892), Jeanna Kim

Master's Theses

The harp is a musical instrument with a uniquely resonating timbre, and a highly specialized expressive niche within the orchestral repertoire. A deeper study of the harp’s metaphorical use is conducted in this report by evaluating the historical context in which the harp emerged, analyzing the timbre of the modern double-action pedal harp, and reviewing the historically significant ensemble works to reveal why composers elected to feature the harp in the orchestra, in lieu of and in conjunction with other instruments. Each of these elements points squarely towards the use of the harp to represent liminal themes and the universal …

Lenstra-Hurwitz Cliques In Real Quadratic Fields, Daniel S. Lopez

Master's Theses

Let $K$ be a number field and let $\OO_K$ denote its ring of integers. We can define a graph whose vertices are the elements of $\OO_K$ such that an edge exists between two algebraic integers if their difference is in the units $\OO_K^{\times}$. Lenstra showed that the existence of a sufficiently large clique (complete subgraph) will imply that the ring $\OO_K$ is Euclidean with respect to the field norm. A recent generalization of this work tells us that if we draw more edges in the graph, then a sufficiently large clique will imply the weaker (but still very interesting) conclusion …

Airborne Doppler Radar Observations Of Pyrocu/Cb Plume Kinematics And Thermodynamics During The 2016 Pioneer Fire, Bruno Rodriguez

Master's Theses

During a period of explosive growth of the Pioneer Fire (Idaho, August 2016), deep pyroconvective plumes were sampled by aircraft. The research aircraft was equipped with both remote sensing and in situ instrumentation, including a W-Band Doppler radar which provided high-resolution vertical velocity retrievals from within the developing pyrocumulus. Being the first direct observations of vertical velocity within a pyrocumulus cloud, they have provided unique insights into the dynamical processes governing pyroconvective environments, with important implications for the fire modeling community. The observations were quality-controlled and corrected for issues such as Doppler velocity aliasing, and the plume’s kinematic structure was …

Application Of Model Predictive Control In Modular Multilevel Converter For Mtpa Operation And Soc Balancing, Mohit Sharma

Master's Theses

In this thesis, a one-step horizon model predictive control strategy (MPC) is implemented in a multilevel modular converter (MMC) to control the speed of an electric vehicle (EV) motor. Maximum torque per ampere (MTPA) and field weakening (FW) control strategies are used to generate reference signals for maximum torque output. The proposed control scheme aims to track the reference signal by independently regulating voltages from the MMC modules. To achieve this, the switches of the MMCs are directly controlled, eliminating the need for a pulse width modulator. A one-step horizon implementation of MPC ensures the robustness of the control system …

Separation Events In Modern Greek, Vasiliki Lydia Rounti

Master's Theses

The semantic domain of separation events has been studied for a number of languages. In this thesis, a study on how Modern Greek expresses separation events and how semantically distinct they are from other languages is presented. This is the first study on the semantic features of separation events for Modern Greek. Furthermore, it is the only study so far on separation events for which data have been gathered from a large number of native speakers. Thus, this thesis not only offers a great insight on the language’s semantic structures, but it also presents more concrete observations and conclusions compared …

The Design And Detailing Of Discontinuous Timberframe Lateral-Force-Resisting Systems, Rhett Kenneth Carlsen

Master's Theses

Discontinuous lateral-force-resisting systems (LFRS) are a type of LFRS in which the path of resistance does not continue directly to the foundation. Discontinuous systems are defined as either in-plane, where the LRFS shifts in the plane of resistance, or out-of-plane, where the shift is perpendicular to the plane of resistance. Discontinuous systems are especially prevalent in lightweight, woodframe structures. Design penalties exist for discontinuous systems, resulting in the specification of larger connections and structural elements. This research includes a review of past experiments to identify any discontinuous LFRS experiments that have been conducted. Through the identification of the existence and …

Everybody Else, Patricia Gaglia

Master's Theses

Everybody Else is a novel that follows the story of the Promise Scholars, a group of math whizzes thrust into the media spotlight after a tech billionaire launches an engineering program at their low-income, predominantly black, high school. The purpose of this novel is to explore contemporary racism in America, the complex relationship between philanthropy and poverty, and the role of technology in instigating social change. Each chapter is a complete story, representing a unique first person perspective as the students navigate the closure of their school, the dissolution of their benefactor’s interest, and their enrollment in an affluent neighboring …

Taking A Stand On One Knee: A Content Analysis Study Of The San Francisco 49ers' National Anthem Protests, Jack Hunter

Master's Theses

Previous communication studies have examined media bias in the coverage of social protest movements and issues of race in sports, but until now there has not been an opportunity to combine the two topics. This study examined media bias in coverage of the San Francisco 49ers’ national anthem protests, from August 2016 to February 2018. Utilizing articles from eight different publications, four from local San Francisco Bay Area outlets, and four from nationally syndicated outlets, a content analysis was conducted to determine whether coverage of the protests exhibited bias against the players and whether local sources were more supportive of …