Digital Commons Network^™

Covalent Inhibition Of Enzyme Sortase A As A New Pathway Against Bacterial Resistance Of Staphylococcus Aureus, Umyeena Bashir

Master's Theses

The continued rise of antibiotic-resistant bacteria, such as MRSA (methicillin-resistant Staphylococcus aureus), has made the discovery of novel antibiotics critical. While bacteriostatic and bactericidal antibiotics inevitably exert evolutionary pressure on bacteria to develop resistance, small molecules that target mechanisms of bacterial virulence present a promising alternative for treatment. Sortase A (Srt A), a cysteine protease of S. aureus, promotes bacterial virulence by covalently attaching proteins such as pilin to the bacterial surface, enabling bacterial adhesion to mammalian cells. Inhibitory studies of this enzyme have gained prominent interest as a new pathway for drug development since blocking this enzyme's ability to …

The Impact Of Sea-Level Rise On Coastal Wetlands Using In-Situ Mesocosm Experiments, Landscape Modeling, And Traditional Ecological Knowledge Mapping, Kelly San Antonio

Master's Theses

Sea-level rise is an escalating threat to coastal wetlands as increased inundation and saltwater intrusion can lead to lowered productivity, decreased biomass, and plant death – and ultimately land loss. In chapter one, I detailed the interactive effects of inundation and nitrogen on two commonly found saltmarsh species, Spartina alterniflora and Spartina patens. I examined productivity and metrics to these stressors using a controlled mesocosm experiment in the western channel of the Pascagoula River, Mississippi. I found varying strategies of growth between species and differing responses between the short- and long-term. Overall Spartina alterniflora performed better with increased inundation …

Evaluation Of Polyvinyl Alcohol (Pva) For Electrospinning Utility In The Blood Vessel Mimic (Bvm) Lab, Logan Vandenbroucke

Master's Theses

Electrospinning has provided the opportunity to create extracellular matrix (ECM) mimicking scaffolds for the development of tissue-engineered constructs. Within Professor Kristen Cardinal’s Blood Vessel Mimic (BVM) Lab, at Cal Poly, there exists a constant demand for innovation and the expansion of polymer types and electrospinning capabilities for its BVM model. Along these lines, the BVM Lab has recently acquired two new electrospinning systems: the Spinbox, a commercially graded electrospinning system, and the Learn-By-Doing system, which was part of a recently completed thesis conducted by Jason Provol. Additionally, recently published literature has demonstrated polyvinyl alcohol (PVA) as a viable option for …

Effects Of Beet Supplements On Cardiovascular Response Using A Noninvasive Blood Pressure Cuff, Nicholas M. Hughes

Master's Theses

A Calibrated Cuff Plethysmography device was built, tested for verification, and used to experiment on human subjects to measure the cardiovascular response of consuming a beet supplement, specifically looking at arterial compliance and pressure-area curves. Each subject was tested four times. A baseline was measured under normal conditions and after five-minute hyperemia conditions. 10 subjects were given 6 ounces of water mixed with either purple Kool-Aid (control), a SuperBeets supplement, or a SuperBeets Sport supplement and after 45 minutes, measurements were taken undergoing normal and hyperemia conditions once more. The verification testing demonstrated the calibration of the device was effectively …

Optimization Of Hydraulic Cylinder Positioning For Wind Tower Hydraulic Erection System, Anbesh Rawal

Master's Theses

Wind produced electricity is a rapidly growing field with wind towers serving as critical components. Conventionally, special cranes are commonly used for wind tower erection. This study explores the use of hydraulic cylinders for small-scale wind tower erection. The thesis aims to find the optimal position of hydraulic cylinder connections in a hydraulic erection system for safe and energy-efficient tower erection and retraction and to enhance the performance and longevity of the system.

Structural analysis was conducted to investigate various hydraulic cylinder positioning configurations, ensuring minimal force exertion while maintaining structural integrity. The study included the selection of locally available …

Perturbation Based Decomposition For Motor Unit Number Estimation, Anna T. Pham

Master's Theses

Motor unit number estimation (MUNE) is of clinical significance because information associated with motor unit populations and the loss of motor units over time are crucial to the diagnosis and monitoring of neuromuscular disorders. The responses of individual motor units can be extracted through incremental stimulation of a muscle group of interest and further analyzed through various methods to compute the MUNE. In this work, the utility of the perturbation-based decomposition by Szlavik will be investigated for this application and compared to the original MUNE approach developed by McComas. Successful decomposition may suggest an alternative method for motor unit number …

Development Of Load Measurement Technique For Arbitrary Shapes, Quintin J. Cockrell

Master's Theses

Obtaining aerodynamic forces and moments about all three orthogonal axes for arbitrary shapes at arbitrary orientations in a fast manner via a measurement technique specific to Cal Poly’s low-speed wind tunnel to continually obtain the forces and moments under quasi-steady conditions is explored. A Continuous Rotation Technique (CR) uses a 6-DOF load cell and stepper motor to rotate an object about an axis for a complete rotation. The forces and moments acting upon the object pass through the stepper motor and interface plates and recorded by the load cell as the object is rotated continuously a finite number of rotations. …

Foundations Of Memory Capacity In Models Of Neural Cognition, Chandradeep Chowdhury

Master's Theses

A central problem in neuroscience is to understand how memories are formed as a result of the activities of neurons. Valiant’s neuroidal model attempted to address this question by modeling the brain as a random graph and memories as subgraphs within that graph. However the question of memory capacity within that model has not been explored: how many memories can the brain hold? Valiant introduced the concept of interference between memories as the defining factor for capacity; excessive interference signals the model has reached capacity. Since then, exploration of capacity has been limited, but recent investigations have delved into the …

Brunet: Disruption-Tolerant Tcp And Decentralized Wi-Fi For Small Systems Of Vehicles, Nicholas Brunet

Master's Theses

Reliable wireless communication is essential for small systems of vehicles. However, for small-scale robotics projects where communication is not the primary goal, programmers frequently choose to use TCP with Wi-Fi because of their familiarity with the sockets API and the widespread availability of Wi-Fi hardware. However, neither of these technologies are suitable in their default configurations for highly mobile vehicles that experience frequent, extended disruptions. BRUNET (BRUNET Really Useful NETwork) provides a two-tier software solution that enhances the communication capabilities for Linux-based systems. An ad-hoc Wi-Fi network permits decentralized peer-to-peer and multi-hop connectivity without the need for dedicated network infrastructure. …

Decentralized Machine Learning On Blockchain: Developing A Federated Learning Based System, Nikhil Sridhar

Master's Theses

Traditional Machine Learning (ML) methods usually rely on a central server to per-
form ML tasks. However, these methods have problems like security risks, data
storage issues, and high computational demands. Federated Learning (FL), on the
other hand, spreads out the ML process. It trains models on local devices and then
combines them centrally. While FL improves computing and customization, it still
faces the same challenges as centralized ML in security and data storage.

This thesis introduces a new approach combining Federated Learning and Decen-
tralized Machine Learning (DML), which operates on an Ethereum Virtual Machine
(EVM) compatible blockchain. The …

Deep Ocean Vehicle Applications And Modifications, Nichole "Nikki" T. Arm

Master's Theses

This project had two primary goals: (1) to explore opportunities to further a deep-ocean vehicle’s reach using alternative pressure spheres, and (2) to implement an existing deep-ocean vehicle (lander) in active scientific research.

I gained a greater understanding of the limitations and design choices made for existing pressure spheres using Finite Element Analysis (FEA). My simplified FEA model predicted sphere failure for the existing 30% Fiber Glass 70% Nylon injection molded spheres at an external pressure of 3,954psi or 2,690m ocean-depth (only a 7.38% error compared to the tested minimum failure depth), so I determined it a valid model. I …

Discourse Analysis In Engineering: Investigating Patterns In Brainstorming Conversations, Aimee Chiem

Master's Theses

Brainstorming is a critical part of the engineering design process and can have a significant impact on the outcomes of the overall project. While research has studied the outcomes of brainstorming and the ideas that teams generate, the role that language and conversation play in these activities is still relatively underexplored. Observing the different ways people use specific types of discourse can reveal how conversations can affect brainstorming itself. To that end, this research aims to answer the following questions:

1) What are the different kinds of discursive moves that students make during engineering brainstorming activities?

2) What patterns or …

Evaluation Of Facilitation On Translocation Outcomes For San Joaquin Antelope Squirrel In The Carrizo Plain, California, Patrick R. Anderson

Master's Theses

In arid and semi-arid environments, burrowing mammals play a key role in increasing landscape heterogeneity through facilitative species interactions. The loss of burrowing mammal populations can consequently lead to negative effects cascading through the ecosystem. It is critical to understand these facilitative interactions for conservation and management. Understanding facilitative interactions may improve wildlife management tools, like translocation, which is often not very successful. To investigate the importance of burrow facilitation on San Joaquin antelope squirrel (Ammospermophilus nelsoni; SJAS) translocation success, I designed a natural experiment with a burrowing facilitator, the giant kangaroo rat (Dipodomys ingens; GKR). I …

A Numerical Modeling Analysis Of The San Francisco Bay And Sacramento-San Joaquin Delta: Riverine, Tidal, And Wind Processes, Drake A. Abrahamsson

Master's Theses

The primary motivation of this study is to analyze the 1D-2DH hydrodynamic model of the San Francisco Bay and Sacramento-San Joaquin Delta (SFBD) outlined in Nederhoff et al. (2021). I compared model water level data to 70 tidal records from the National Oceanic and Atmospheric Association (NOAA), the United States Geological Survey (USGS), the California Data Exchange Center (CDEC), and from local municipalities throughout the Bay Area to investigate how the model captures water levels and tidal constituent amplitudes. While the Nederhoff et al (2017) model analyzed an extended time period from 1950-2019, I analyzed M2 amplitude and tidal water …

Launch Vibration Attenuation For In-Space Assembly Cargo, Jered Bell

Master's Theses

This thesis investigates the implementation of a passive isolator with a pressurized air cushion for spacecraft payloads in mission architectures implementing in-space assembly technologies. A pressurized air bed capable of briefly surviving the space environment for cargo delivery was prototyped and experimentally evaluated for launch vehicle vibration dynamics resulting in a 72%, 93%, and 88% reduction in experienced GRMS loads for the X-Axis, Y-Axis, and Z-Axis, respectively. A preliminary Total Mass Loss evaluation of the Low-Density Polyethylene Film utilized for the air bed resulted in a mass loss of 0.7%, indicating that commercial off-the-shelf films might require minimal modification for …

Nanoparticles And The Environment: Biopolymer Grafted Cellulose And Screen-Printed Carbon Nanotube Composites, Dominique Henry Porcincula

Master's Theses

A host of environmental issues will define the state of the environment in the 21^st century, with plastic pollution and water shortages among them. While solutions to these problems require large-scale, multipronged solutions, one way we can address them is through material innovation and the use of nanoparticles.

In the first project, we address the issue of plastic pollution by creating nanocomposites of biodegradable polymers (PLA and PCL) with cellulose nanofibrils. Here, PLA and PCL are grafted from the surface of cellulose nanofibrils via ring-opening polymerization of cyclic ester monomers. Polymer-grafted cellulose (PGC) is characterized with structural analysis, solubility …

Fabrication Of Alumina Membranes From Uv Resin– Alumina Particle Slurries, Dominique Henry Porcincula

Master's Theses

Ceramics membranes are made in a wide variety of different techniques using a wide variety of different materials. However, many of the common techniques utilize a slurry of ceramic particles, additives, and either organic solvent or water that is shaped into a membrane, left to dry, and then sintered together. Drying is a time consuming process, often requiring several hours for the liquid medium to evaporate. Defect formation caused by development of partial pressures across the drying membrane, including cracks and warpage, also typically occurs during the drying process. To address this, slurries of ceramic particles made with a low …

Dance And Emotional Intelligence: Mitigating Anxiety In The Modern Teen, Bridget Morris

Master's Theses

The purpose of this thesis study was to find if emotional intelligence could be increased through participation in dance classes, and if that increase of emotional intelligence could mitigate anxiety in teens today. The researcher held four dance classes with a total of five 15–16- year-old volunteer participants, all with dance experience. Two research instruments were used and data were analyzed quantitatively. The research questions explored were: Q1 What immediate effects do participating in a dance class have on emotional intelligence? Q2 Which components of emotional intelligence develop the most from participating in dance? The data demonstrated that participation in …

Understanding The Physical Oceanography Of The Mississippi Sound: Observational Climatologies, Trends And Relationships In The Sound And Comparison Of The Surface Currents Of Ocean Models With High Frequency Radar Data, Tasnim Islam

Master's Theses

The Mississippi Sound is a shallow lagoon-type water estuarine system that is a major part of Northern Gulf of Mexico (NGOM) separated from the shelf waters by a series of barrier islands. It is mostly characterized by its strong weather fronts, storms and hurricanes that affect the hydro-morpho-dynamics of the region. This includes strong impact on the current circulation, flow pattern, sedimentation etc. Understanding the physical oceanography of the Sound through observing the climatologies of the ocean parameters, discovering the trends and relationships and knowing the circulation pattern is extensively important since the Sound is an indisputable ecological resource to …

How Sewage Pollution Affects Distribution And Life History Traits Of The Southern House Mosquito, Culex Quinquefasciatus, Catherine Dean Bermond

Master's Theses

Eutrophication from wastewater can cause fluxes of nutrients like and carbon and nitrogen in urban waters. One source of nitrogen in freshwater is from untreated sewage discharging from septic tanks. This causes a variety of environmental issues including harmful algal blooms, harming natural predators, and increased production of mosquitoes. Culex quinquefasciatus is the vector of West Nile Virus in St. Tammany Parish, Louisiana. St. Tammany Parish has over 600 miles of septic ditches, which receive effluent directly from aerated treatment units. Culex quinquefasciatus oviposit in polluted waters, and larvae perform well in water with high levels of decomposing organic matter. …

Evaluating Habitat Use By Nekton In Widgeon Grass (Ruppia Maritima), Shoal Grass (Halodule Wrightii), And Unvegetated Bottom Habitats In The Grand Bay National Estuarine Research Reserve, Jessica Woodall

Master's Theses

Seagrass beds support high biodiversity and animal abundance, serve as feeding grounds for a variety of animals, offer shelter from predation, and act as a nursery habitat for juveniles. The species composition of seagrass beds can impact their use as habitat by animals. Two common species of seagrass in the Gulf of Mexico are Ruppia maritima (widgeon grass) and Halodule wrightii (shoal grass). The shallow coastal waters of the Grand Bay National Estuarine Research Reserve (NERR) support both species, but the use of each seagrass as habitat by nekton is poorly understood, which can limit management decision-making. Nekton communities were …

Geospatial Business Location Implications For Mississippi-Based Nissan Group Changing To Electrical Vehicle (Ev) Production, Sudheer Galla

Master's Theses

This research examines the shift from conventional Internal Combustion Engine (ICE) to Electric Vehicle (EV) production in the automotive industry. Utilizing the JobsEQ Input-Output model, it reveals complex economic interdependencies and highlights the concentration of Nissan Tier 1 suppliers around its Canton manufacturing operations in the Midwest and Southern U.S., indicating an efficient supply chain and regional economic impact.

In Mississippi, the transition to EV production is projected to create 4,415 jobs and increase compensation by $295.5 million, presenting a competitive edge in automotive employment opportunities. However, it emphasizes the need to retain and upskill the existing workforce, especially for …

Estimation Of Angular Response Curves Using A Phase Differencing Bathymetric Sonar, Kandice Gunning

Master's Theses

Although Multibeam Echosounders (MBES) backscatter products like Angular Response Curves (ARC) and mosaics have been used and applied to different sediment types as classification tools, little is known about the extraction and interpretation of ARCs using Phase Differencing Bathymetric Sonar Systems (PDBS). This thesis aims to use an across-track approach to develop a method for extracting ARCs from georeferenced bathymetric data using PDBS.

Three different data collection surveys were conducted using a PDBS operating at 540kHz. Using only the bathymetric data, initial ARCs from 3 different intensity classes (high, medium, and low) were successfully extracted, the results of which attempted …

Using Behavior Bingo To Increase Academically Engaged Behavior In A General Education Elementary School Population, Faith Elizabeth Hamilton

Master's Theses

Spending instructional and work time addressing disruptive behaviors in the classroom is a common complaint of many teachers. The literature suggests that group contingencies are beneficial for improving behavior on a classwide and individual level. The current study investigated Behavior BINGO, a group contingency intervention whose research is in its infancy, and its effectiveness on increasing academically engaged behaviors and decreasing disruptive behaviors on a classwide basis. This study utilized a nonconcurrent multiple baseline design in three 2^nd grade, general education classrooms in a public school.

An Analysis On Wildfire Mitigations Employed By Utilities In California, Evan Luu

Master's Theses

As climate change continues to worsen, environmental effects are felt by many people around the world. In California, some of its most damaging wildfires have been found to be started by utilities. As the state continues to suffer from worsening wildfire conditions, the utilities need to implement a variety of wildfire mitigations to help reduce the risk of wildfires that can affect the state and its residents. This paper analyzes the effectiveness of four mitigations employed across three California utilities and suggests potential ways for the mitigations to be used together. The technologies evaluated are covered conductor, rapid earth fault …

Improvement Of Rotation Capacity Of Composite Beam-To-Hss Column Connections Using External Horizontal Stiffeners, Mahdi Afshar Arjmand

Master's Theses

Improvement of Rotation Capacity of Composite Beam-to-HSS Column Connections Using External Horizontal Stiffeners

Mahdi Afshar Arjmand

This thesis focuses on the analysis of out-of-plane deformation (OOP) in column flange located in the panel zone of composite beam-to-column steel connection, as a critical aspect of steel structural engineering. This type of connection is an integral component of steel structures, and understanding their behavior is essential for ensuring safety and performance. The investigation involves examining the causes, factors influencing, and potential mitigation strategies for out-of-plane deformation of HSS flange column in these connections. Beam-to-column connections play a vital role in transferring loads …

An Ecological Assessment Of An Endangered Lichen From Central California: Sulcaria Isidiifera, The Splitting Yarn Lichen, Elijah C. Balderas

Master's Theses

Lichens are an underrepresented group of organisms when it comes to both research and conservation efforts. At the same time, lichens face increasing threats from anthropogenic sources including wildfires, climate change and urbanization. With this thesis, I seek to ascertain and publish ecological data on a critically endangered lichen to inform future conservation designations and efforts to preserve and protect the species in perpetuity.

In Chapter 1, I provide an ecological overview of the known range of Sulcaria isidiifera and assess the status of Sulcaria isidiifera via a population count. Sulcaria isidiifera occurs in maritime chaparral habitat in the Los …

The Tidal Prism, Viable Eelgrass Habitat, And The Effects Of Sea Level Rise In Morro Bay, Kaden A. Caliendo

Master's Theses

The tidal prism, or the volume of water exchanged from the sea to an estuary from mean low to mean high tide, influences system hydrodynamics and ecological functioning. Since 1884, the tidal prism in Morro Bay, California has been estimated to be decreasing over time due to sedimentation from upstream practices. What is the current tidal prism in Morro Bay and how will that change with sea level rise? How will eelgrass respond to rising sea levels?

For this study, inexpensive tidal gauges were deployed at four locations in Morro Bay from March to August 2023 to measure spatially varying …

Parametric Optimization Of A Wing-Fuselage System Using A Vorticity-Based Panel Solver, Chino Cruz

Master's Theses

Aerodynamic topology optimization is a useful tool in the aerodynamic design pro-
cess, especially when looking for marginal gains within a design. One example is
a turboprop racer concept aircraft that is designed with the goal of breaking world
speed records. An optimization framework was developed with the intention of later
being applied to this design. In the early design stages, the optimization framework
must focus on quicker methods of drag estimation, such as a panel codes. The large
number of design variables in topology optimization can exponentially increase func-
tion evaluations and thus computational cost. A vorticity-based panel solver …

Exploring The Interactions Of Copper High Aspect Ratio Structures (Cuhars), A Novel Metal-Organic Biohybrid Nanocomposite, With Chondrocytes, Tasneem Khasru

Master's Theses

Osteoarthritis (OA) is a prevalent degenerative joint disorder characterized by cartilage lesions and subchondral bone destruction (1). Addressing OA is challenging due to cartilage's avascular and aneural nature, unique cellular arrangement, and dense extracellular matrix (ECM). Current pharmacological treatments are mainly symptomatic and are unable to halt disease progression or reverse cartilage damage . Surgical interventions also come with complications. Tissue-engineered grafts using biomaterials and molecular manipulation have emerged as potential treatments for cartilage regeneration. Copper-containing scaffolds have shown promise in bone and cartilage regeneration. Nanotechnology offers new avenues, with nanoparticles and nanofibers being used in tissue engineering research. However, …