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Using Zebrafish As A Model System For Studying The Autism Risk Gene Adnp In Early Embryonic Development, William Theune

Master's Theses

Autism Spectrum Disorders (ASD) are a group of debilitating neurodevelopmental disorders, estimated to affect 1 in 54 children. Despite the identification of many autism-risk genes, the underlying mechanisms for ASD remain unclear. One of the most common forms of ASD is associated with de novo mutations in the ADNP gene (Activity-Dependent Neuroprotector Homeobox; accounting for 0.17% of ASD individuals). Termed ADNP syndrome, this disorder is characterized by intellectual disability, facial dysmorphia, and is comorbid with multiple organ system deficits. We set out to use zebrafish as a model organism to gain mechanistic insights into ADNP function. Zebrafish have the advantage …

An Examination Of Average Pretrial Risk Assessment Scores Between Drug And Violent Offenders, Annaliese Engler

Master's Theses

With an overwhelming number of offenders in prison for a drug-related offense (Federal Bureau of Prisons, 2020), evidence-based practices are as important as ever to help properly determine incarceration. Prior literature has shown that pretrial release decisions impact the final outcome of the case and tools used for such a decision should be evaluated and scrutinized (Johnson et al., 2014; Oleson et al., 2014). It seems that although violent offenders pose more of a physical threat to the community than drug offenders, the pretrial risk assessment tools cater towards pushing drug offenders towards a higher risk score. Using data from …

Evaluating Changes In Reactive Oxygen Species (Ros) As A Plausible Mechanism Underlying The Effect Of Noise On The Dopamine System In The Hub For Central Auditory Processes, Bridget Doe

Master's Theses

Excessive exposure to noise leads to hearing loss but the mechanism by which this happens remains unknown. Presently, dopamine, a neurotransmitter widely known for its involvement in learning and reward-based actions, has also been linked to auditory processes in the inferior colliculus, a principal integration center for hearing and related processes. Preliminary data from our research laboratory, has demonstrated that exposure to deafening noise leads to decreased dopamine release in the inferior colliculus, thus implicating the dopamine system in hearing loss. One plausible mechanism underlying this noise-induced alteration in dopamine neurotransmission could be via excessive production of reactive oxygen species …

Recognizing Race: The Impact Of Twentieth-Century Feminist Movements On Race Relations In West Germany, Lindsey Stobaugh

Master's Theses

After World War II, many West German women had a difficult time coming to terms with the atrocities that the National Socialist leadership committed during that war, as well as their own participation in the Party. Discussions of the roles of women within twentieth-century society began to grow in West Germany as the new women’s movement (die Neue Fraenbewegung) emerged from 1960s student protests. This movement included primarily middle-class white German women. They often dismissed their participation in Party racism by framing themselves as victims of a patriarchal regime. As German women discussed these matters, they ignored the …

Effects Of Religiosity And Self-Control On At-Risk Adolescent Substance Use And Delinquency, Lauren Burns

Master's Theses

The adolescent period is one of growth, increased autonomy, and increased risk-taking. Common examples of these risky behaviors include substance use and engagement in delinquent activity. Adolescent substance use and delinquent behavior can lead to a range of negative outcomes, some of which carry into adulthood. These negative consequences are especially evident among at-risk adolescents such as those who have dropped out of school or who come into contact with the juvenile justice system, as these youth often demonstrate higher rates of substance use and delinquent behavior. Understanding the nature and development of these problem behaviors is essential to developing …

Comparing The Efficacy Of Two Affirmation Interventions To Reduce Stereotype Threat Effects On Women’S Math Performance, Alicia Macchione

Master's Theses

Research demonstrates when math-based gender stereotypes are activated (i.e., men are better at math than women), women display comparatively poorer math performance than men, a phenomenon referred to as stereotype threat. The current thesis project evaluated two self-affirmation interventions designed to reduce the effects of stereotype threat on women’s math performance. Participants completed a math test under one of four conditions: control (no explicit stereotype activation), stereotype threat (activation of gender performance stereotype) or stereotype threat combined with one of two self-affirmation manipulations. Women in the affirmation conditions either read about women’s greater verbal or relational ability and were asked …

Underrepresentation Of Women In American Politics, Lindsey Crane

Master's Theses

In this project I studied why women are underrepresented in state legislatures. I conducted a data set ranging from 2000 to 2018 for forty-three US states, analyzing the percentage of women who won and the number of women who ran in the elections. Using this data, I found evidence that personal life choices have the most effect on rather women want to run for political office and successfully pursue political careers. Having this specific quantitative dataset, the study provides a better understanding to why women are still widely underrepresented on the state level. I also find that my independent variables …

The Effects Of Vertical Diel Thermocline Movement On The Settling Of Marine Snow In A River Influenced Marine Environment, Nadine Doiron

Master's Theses

To evaluate the role of diel heating and cooling cycles in the upper water column in marine snow settling processes, particle distribution data from 15 marine snow camera casts were analyzed to examine variations in aggregate abundance, ellipsoidal volume, and particle elongation with depth. Data were collected during two cruises aboard R/V Endeavor in the Gulf of Mexico (GoM), EN620 in 2018 and EN642 in 2019. To develop a deeper understanding of daily patterns in marine snow settling, data were collected at the beginning and end of daily heating cycles. Temperature data were obtained by camera mounted Seabird SBE19+ model …

Small-Scale Resuspension And Redistribution Of Surface Sediments In The Northeast Gulf Of Mexico, Austin Harris

Master's Theses

Following the release of ~4.9 million barrels of oil into the Gulf of Mexico from the Macondo wellhead, a vast area of the seafloor contained recently deposited marine sediments contaminated by the oil spill. The initial deposition of these contaminated marine sediments was likely not the end of the journey for the particles. Downslope gravitational processes and events of increased current speed in the deep ocean setting can result in recently deposited sediments to resuspend and be moved laterally with the current flow, increasing the area effected by the oil spill. Erosion experiments performed in a closed-loop resuspension flume were …

How Historic Shipwrecks Influence Dispersal Of Deep-Sea Microbiomes, Rachel Moseley

Master's Theses

This thesis investigates how historic shipwrecks potentially shape dispersal of deep-sea microbiomes. Processes impacting dispersal of microbiomes around artificial structures in the ocean and how they connect to other deep-sea habitats is unknown. Dispersal processes are explained for some macroorganisms by theories in ecology, specifically the Theory of Island Biogeography and the Wooden Stepping Stone hypothesis. These have not been investigated for microorganisms, and thus this work will probe if they are applicable to microbial biogeography in this habitat. Experiments were conducted to establish new “island-like” features in near proximity (25-125 m) to wooden-hulled historic shipwrecks in the northern Gulf …

Trophic Ecology Of Mesopelagic Fish Larvae And Juveniles In The Northern Gulf Of Mexico, Emily Gipson

Master's Theses

The deep-pelagic environment encompasses ocean waters below 200 m depth, and comprises 90% of the volume of the Gulf of Mexico. Deep-pelagic fishes are important prey for many oceanic consumers, but relatively little is known about their early life history, including larval fish trophic ecology. An understanding of the role deep-pelagic fish larvae have in oceanic food webs is important in the development of ecosystem models that examine the connectivity (via vertical migrations) between the deep-pelagic and epipelagic environments with respect to trophic interactions, nutrient cycling, and carbon sequestration. In this study, archived plankton samples collected during 2010 and 2011 …

Warm Or Chilly? An Assessment Of The Relationships Between Campus Climate And Gender Identity Among Transgender And Nonbinary College Students, Emma Allen-Morgan

Master's Theses

This research is a mixed-methods analysis of the relationship between campus climate and gender identity for transgender and nonbinary college students. Snowball and convenience sampling were used to obtain a sample of trans and nonbinary college students. Thirty surveys and six interviews were completed. I applied Bronfenbrenner’s ecological theory and Risman’s gender structure theory to analyze how macrosystems, microsystems, and the chronosystem (time) affected trans students’ gender identity, whether positively or negatively. Participants were ambivalent about the effects of college on their gender identity; on one hand, college gives trans students freedom from their previous environments. However, participants described a …

Indirect Impact Of Soil Microbial Communities On Plant-Aphid Interactions, Zachary Tiemann

Master's Theses

The community dynamics of soil micro-environments are influenced by root, fungal, and bacterial exudates that can preferentially select for functional classes of microbes. Arbuscular mycorrhizal fungi (AM) fungi and plant growth promoting rhizobacteria (PGPR) are members of this community form symbiotic relationships with plants and play an important role in triggering induced systemic resistance (ISR), resulting in defensive “priming.’ Consequently, ‘primed’ plants can activate stronger and faster defense responses to future attacks by pathogens and insects. The biological system in this study involved four genotypes of barrel medic plants (Medicago truncatula), pea aphids (Acyrthosiphon pisum), and microbial communities present in …

Characterizing Of Monoamine Neurotransmitters At The Hub Of The Central Auditory System, Patrick Wilson

Master's Theses

In the United States, loss of hearing impacts approximately 48.1 million people. The cumulative effects of noise are experienced in every area of society whether occupational, environmental, or through aging. A number of studies have linked noise induced hearing disorders to changes in spontaneous neuronal activity in certain auditory brain regions, especially, the inferior colliculus, a region that integrates auditory inputs from both ascending and descending pathways. However, the exact neurochemical basis of these disorders remains elusive. Currently, there are several compelling pieces of evidence that implicate the monoamine neurotransmitter, dopamine, in auditory processing. Based on this data, we have …

Investigating The Role Of Atp-Binding Cassette Transporters In Drosophila Melanogaster Testis Stem Cells, Israel J. Wipf

Master's Theses

Multidrug resistance is among the most pressing obstacles in cancer treatment today. Resistance is thought to arise from the ability of cancer stem cells to efflux therapeutic molecules using a collection of membrane proteins called ATP-binding cassette (ABC) transporters. There is strong interest in targeting ABC transporters to preserve and improve drug efficacy and reduce cancer recurrence. Many studies have been performed in vitro using cultured cell lines, but currently there is a lack of simple models in which to study ABC transporters in vivo. As a solution, I propose to use the fruit fly Drosophila melanogaster for the study …

Resistance To Rattlesnake Venoms In An Eastern Colorado Rodent Community, Neil Balchan

Master's Theses

The grasslands of eastern Colorado are inhabited by two species of rattlesnakes, the Prairie Rattlesnake (Crotalus viridis) and the Desert Massasauga Rattlesnake (Sistrurus tergeminus edwardsii). An array of rodent species, both native and introduced, also occupy these grasslands, and serve as a varied prey base for the previously listed rattlesnakes. Predator-prey interactions in this system gain an additional level of complexity due to the presence of venoms, a chemical arsenal possessed by both rattlesnakes to incapacitate their prey. Rodents in other systems have demonstrated resistance to snake venoms, and there is potential for a similar dynamic to be present in …

Cannabigerol Attenuate Methionine/Choline Deficient Diet- Induced Non-Alcoholic Steatohepatitis Symptoms In C57bl/6 Male Mice Via Modulating The Expression Of Cannabinoid Receptors, Nouf Aljobaily

Master's Theses

Non-Alcoholic Steatohepatitis (NASH) is the advanced and more aggressive form of Non-Alcoholic Fatty Liver Disease (NAFLD). NASH is associated with severe hepatic fibrosis and inflammation. Methionine/choline deficient (MCD) high fat diet is known to induce NASH in a short period of time without showing signs of metabolic syndrome. Cannabigerol (CBG) is a plant-derived, non-psychotropic cannabinoid that has a potential anti-inflammatory effect. Other hemp extracts reduce the progression of NAFLD to NASH, whereas the impact of CBG on NASH is still unknown. Therefore, these studies aim to 1. Evaluate the therapeutic potential of CBG on reducing hepatic steatosis and fibrosis. 2. …

Lotus: A Web-Based Computational Tool For The Preliminary Investigation Of A Novel Mst Method Utilizing A Library Of 16s Rrna Bacteroides Otus, Ginger Dewitte

Master's Theses

Microbial Source Tracking (MST) is a field of study that attempts to identify the source of fecal contamination in waterways in order to assist with development of remediation strategies. Biologists at Cal Poly Center for Applications in Biotechnology (CAB) are developing a new MST method using microbes from the genus Bacteroides. Bacteroides species are host-specific microorganisms that can theoretically be used to trace back to a single host species. After fecal samples are collected, biologists use Next-Generation Sequencing (NGS) techniques to obtain only the genetic sequences of microorganisms belonging to the phylum Bacteroidetes. Investigators hypothesize that similar sequences belong …

An Interdisciplinary Approach: Schizophrenia Derails Heteronormative Expectations In Psychological Narratives 2021, Bobbie Jo Weaver

Master's Theses

Required introductory psychology courses teach students a general and oversimplified version of the immense number of subfields within Psychology studies, much like introductory literature classes compress different genera throughout history into a miniscule number of “representative” texts. Nevertheless, these footholds generate an entryway into a whole new world of (specialized) exploration. Reading a text such as The Quiet Room: A Journey out of the Torment of Madness by Lori Schiller and Amanda Bennett provides a window for many students to crawl into one of Psychology’s darkest shadows, the field of abnormal psychology. Schiller’s non-fictional memoir, The Quiet Room, tells readers …

The Role Of Med12, Notch1, And Notch3 In Adipose Stem Cell Self-Renewal And The Integrated Use Of Stem Cells In Public Educational Materials, Rebecca Hodnett

Master's Theses

Human adipose-derived mesenchymal stem cells offer significant therapeutic potential as an ethically sound, easily accessed source of adult stem cells. To harness their medically relevant properties, it is necessary to understand the mechanisms that control their fate. Cell state and differentiation of stem cells is determined by interactions of signaling pathways, chromatin modifiers, and transcription factors working to regulate cell-type specific gene expression profiles. Specifically, both the MED12 subunit of the Mediator complex and the Notch signaling pathway are known to individually influence hASC self-renewal. We investigated the relationship between Notch signaling and transcriptional cofactor, MED12, to elucidate a potential …

The Effects Of Increased Nacl Salinization On Riparian Plant Productivity And Decomposition Processes, Connor Gruntz

Master's Theses

NaCl salinization in inland ecosystems is increasing globally due to anthropogenic activity and may alter organism abundances and ecosystem processes. Because sodium is an essential nutrient for heterotrophs but not required by autotrophs, this salinization can alter species abundance and performance. Riparia may be particularly sensitive to increases in salinization. Here, I examined the Sodium Subsidy-Stress Hypothesis, which states that deviations from an organism’s optimal nutrient target can decrease abundance and performance, by studying the effects of NaCl salinization on plant chemistry, productivity, and decomposition rates in riparia. First, I selected three focal common riparian plants (water oak: Quercus nigra, …

Relationship Between Calcium Consumption From Dairy And Non-Dairy Sources On Bone Mineral Density Of College Students, Andrew M. Pescovitz

Master's Theses

Dairy product consumption has been on the decline for decades in the US and worldwide, and the increase in plant-based substitutes has grown substantially. Osteoporosis and the risk of bone fractures are serious public health issues. To date, little scientific-based studies have compared the relationship between calcium intake from dairy sources vs non-dairy sources and bone mineral density. The purpose of this study is to compare the relationships between calcium consumption from dairy vs. non-dairy sources and bone mineral density among college students. There was a total of 66 participants in the study including 15 (23%) males and 51 (77%) …

Natural And Experimental Noise Affects Acoustic Communication In Songbirds, Veronica Arlene Reed

Master's Theses

Background noise is ubiquitous and can impair acoustic communication and influence signaling behavior in animals. Despite evidence demonstrating myriad effects of anthropogenic noise on animal communication, precisely how natural background noise influences communication and behavior remains unclear. Yet, natural sources of background noise, such as rushing rivers or crashing ocean surf, share similar power spectra to sources of anthropogenic noise and can occur at high amplitudes, potentially masking acoustic signals.

To investigate the effects of water-generated noise on songbird behavior, we experimentally broadcast landscape-level playbacks of ocean surf and river noise in coastal California, USA, and riparian habitat in Idaho, …

Associations Between Parenting Stress, Feeding Practices, And Child Eating Behaviors During The Covid-19 Pandemic, Maria G. Gonzalez

Master's Theses

The COVID-19 pandemic provides an important opportunity to understand how parenting stress during social crisis may predict child feeding practices and perceptions of child mealtime behaviors. The objective of the present study was to explore whether parents’ perceived increases in and overall levels of parenting stress during the pandemic were associated with controlling feeding practices and perceptions of child eating behaviors. Parents (n = 284) of 4–6-year-old children completed a cross-sectional online survey between March and April 2020. The survey assessed parents’ perceived change in parenting stress during the onset of the pandemic and levels of parenting stress during …

Comparing Radiation Shielding Potential Of Liquid Propellants To Water For Application In Space, John Czaplewski

Master's Theses

The radiation environment in space is a threat that engineers and astronauts need to mitigate as exploration into the solar system expands. Passive shielding involves placing as much material between critical components and the radiation environment as possible. However, with mass and size budgets, it is important to select efficient materials to provide shielding. Currently, NASA and other space agencies plan on using water as a shield against radiation since it is already necessary for human missions. Water has been tested thoroughly and has been proven to be effective. Liquid propellants are needed for every mission and also share similar …

Node Classification On Relational Graphs Using Deep-Rgcns, Nagasai Chandra

Master's Theses

Knowledge Graphs are fascinating concepts in machine learning as they can hold usefully structured information in the form of entities and their relations. Despite the valuable applications of such graphs, most knowledge bases remain incomplete. This missing information harms downstream applications such as information retrieval and opens a window for research in statistical relational learning tasks such as node classification and link prediction. This work proposes a deep learning framework based on existing relational convolutional (R-GCN) layers to learn on highly multi-relational data characteristic of realistic knowledge graphs for node property classification tasks. We propose a deep and improved variant, …

A Method For Visualizing The Structural Complexity Of Organizational Architectures, Jacob Michael B. King

Master's Theses

To achieve a high level of performance and efficiency, contemporary aerospace systems must become increasingly complex. While complexity management traditionally focuses on a product’s components and their interconnectedness, organizational representation in complexity analysis is just as essential. This thesis addresses this organizational aspect of complexity through an Organizational Complexity Metric (OCM) to aid complexity management. The OCM augments Sinha’s structural complexity metric for product architectures into a metric that can be applied to organizations. Utilizing nested numerical design structure matrices (DSMs), a compact visual representation of organizational complexity was developed. Within the nested numerical DSM are existing organizational datasets used …

Studying The Efficacy Of An Injectable 3-Dimensional Fibrin Extracellular Matrix To Characterize The Effects Of Antitumor Agents On Sw620 Cells In A Microfluidic Device, Thèo Anastos

Master's Theses

Colorectal cancer is the third most common cancer in the United States and there is currently a lot of research going into new antitumor agents to kill the cancer. One method for replicating the tumor response to a drug in vivo is by creating an in vitro drug testing model to replicate the in vivo condition. This research project was conducted to determine the efficacy of testing tumor cultures in a microfluidic device as a way to provide accurate drug responses in vitro instead of using in vivo subjects in clinical trials. A total of four experiments were conducted with …

Computational Bone Mechanics Modeling With Frequency Dependent Rheological Properties And Crosslinking, Timothy G. Moreno

Master's Theses

Bone is a largely bipartite viscoelastic composite. Its mechanical behavior is determined by strain rate and the relative proportions of its principal constituent elements, hydroxyapatite and collagen, but is also largely dictated by their geometry and topology. Collagen fibrils include many segments of tropocollagen in staggered, parallel sequences. The physical staggering of this tropocollagen allows for gaps known as hole-zones, which serve as nucleation points for apatite mineral. The distance between adjacent repeat units of tropocollagen is known as D-Spacing and can be measured by Atomic Force Microscopy (AFM). This D-Spacing can vary in length slightly within a bundle, but …

Biological Semantic Segmentation On Ct Medical Images For Kidney Tumor Detection Using Nnu-Net Framework, Andres Bergsneider

Master's Theses

Healthcare systems are constantly challenged with bottlenecks due to human-reliant operations, such as analyzing medical images. High precision and repeatability is necessary when performing a diagnostics on patients with tumors. Throughout the years an increasing number of advancements have been made using various machine learning algorithms for the detection of tumors helping to fast track diagnosis and treatment decisions. “Black Box” systems such as the complex deep learning networks discussed in this paper rely heavily on hyperparameter optimization in order to obtain the most ideal performance. This requires a significant time investment in the tuning of such networks to acquire …