Digital Commons Network^™

Three-Dimensional Staging Series Of Anolis Sagrei, Lilian Epperlein

Master's Theses

With the rapid rise of Evo-devo and the concurrent development of new imaging techniques, comparative studies of development have accelerated over the last decade. Squamates, lizards and snakes, lack a traditional experimental model species for developmental investigations, yet are important for understanding fundamental evolutionary questions because of their remarkable diversity. However, several squamate species have growing communities of biologists building new resources for comparative and experimental studies of lizard development. Creation of detailed embryological atlases for these species will help promote their advancement. In the past, scientists have had to rely on destructive methods to take apart biological samples which …

Bisacodyl Limits Chikungunya Virus In Vitro And Is Broadly Antiviral, Natalie June Lomascolo

Master's Theses

Identifying novel antivirals requires significant time and resource investment, and the continuous threat of viruses to human health necessitates commitment to antiviral identification and development. Developing antivirals requires years of research and validation, and recent outbreaks have highlighted the need for preparedness in counteracting pandemics. One way to facilitate development is to repurpose molecules already used clinically. By screening such compounds, we can accelerate antiviral development. Here, we screened compounds from the National Institutes of Health’s Developmental Therapeutic Program for activity against chikungunya virus, an alphavirus that is responsible for a significant outbreak in the Americas in 2013. Using this …

Elucidating The Function Of An Evolutionarily Conserved Col2Α1 Isoform During Vertebrate Development, Charles Lantz H. Ingersoll

Master's Theses

ABSTRACT

Formation of skeletal structural elements during vertebrate embryogenesis is largely a

conserved evolutionary process that employs numerous conserved genes and pathways. One

critical gene in this process is the highly conserved vertebrate Type II Collagen alpha 1 (col2α1).

It produces multiple splice isoforms that have different functions and expression patterns during

skeletogenesis. Two of the primary isoforms are of great interest; variant 1, the primary

embryonic isoform, and variant 2, a post embryonic isoform. While the more commonly known

post-embryonic isoform has been well studied over the last 30 years, identification of a unique

role for the evolutionarily conserved …

Development And Characterization Of A Tissue-Specific Reporter Line For Monitoring Circadian Clock Transcriptional Activity, Lilyan Mather

Master's Theses

Daily rhythms in physiology and behavior are produced by a circadian system consisting of a central clock located in the brain and peripheral clocks located in various peripheral tissues. Circadian clocks track time of day through the presence of a molecular clock that functions as a transcriptional-translational feedback loop. In the nucleus, the transcription factors CLOCK (CLK) and CYCLE (CYC) activate transcription of clock genes period (per) and timeless (tim). PER and TIM proteins slowly accumulate in the cytoplasm before entering the nucleus, where they inhibit CLK and CYC, and therefore repress their own transcription. Degradation of nuclear PER and …

A Process Evaluation Of Aunt Mary’S Storybook: Seeking To Improve The Relationship Between Incarcerated Parents And Their Children Through Literacy, Scott Charles Mcwilliams

Master's Theses

In response to the rise of incarceration in jails and prisons, and the number of children adversely impacted by their parents’ incarceration, in 1993, a nonprofit organized called Companions Journeying Together (“CJT”), created the Aunt Mary’s Storybook (AMS) program to foster positive connections between incarcerated parents to their children. What began as a Christmastime program for mothers in the Cook County Jail has grown into a year-round program operating in prisons and jails throughout Illinois. AMS provides those incarcerated with an opportunity to record themselves reading a book to their children, and AMS then mails/transmits the recording along with a …

Positive Affect Facilitates Reduction In Depression Symptoms During A Mobile Mindfulness-Based Intervention, Andrew Rauch

Master's Theses

Depression negatively impacts the lives of many, and the rates are continuing to rapidly increase. Identifying patterns of modifiable affective psychological mechanisms that contribute to reducing depression symptoms is critical to improve the effectiveness of wellbeing interventions for individuals with depressive disorders. Depression has been characterized by valanced patterns of low positive affect and high negative affect, yet the extent to which these relative patterns of affectivity change in response to intervention remains less clear. The present study evaluated affective patterns in college students (n = 127) with elevated depression symptoms who participated in a randomized controlled trial (RCT) of …

The Role Of Identification In Black Individuals' Goals To Challenge Racism Through Confrontation And Collective Action, Efrata Sasahulih

Master's Theses

Anti-Black racism continues to harm Black individuals in the United States and throughout the world (e.g., police brutality, medical racism: Hoffman, Trawalter, Axt, & Oliver, 2016; Smiley & Fakunle, 2016; Stuber, Meyer, & Link, 2008). Psychological research has investigated the roots of prejudice and strategies to mitigate its negative consequences, including confrontation and collective action. However, research in this area has largely focused on ways that these strategies pose additional risk for targets of prejudice. This research determined if Black people who endorse individual-benefiting or group-benefiting goals following an instance of anti-Black racism use confrontation and collective action, respectively, to …

Comparison Of Feeding Functional Morphology Development In North American Esocids, Cheryl Theile

Master's Theses

Esocidae is a family of freshwater fishes within order Esociformes known for its long, cylindrical bodies, duck-bill snouts, and an impressive number of sharp teeth. There are four species, including two subspecies, in North America with areas of overlap of their geographic distribution. These ambush predators change diets from invertebrate prey to vertebrate prey during their first year of life, and the timing of this shift varies between species. In particular, the sister species Esox lucius (Northern Pike) and Esox masquinongy (Muskellunge) overlap in the upper Midwest, but E. masquinongy transitions to a vertebrate diet sooner in its development than …

Delayed Testosterone Treatment In A Rodent Model Of Repeat Mild Traumatic Brain Injury Attenuates Mechanisms Of Cellular Death In The Vestibular Nucleus, Michael Volyanyuk

Master's Theses

A common complaint after repetitive mild traumatic brain injury (rmTBI) is vestibular dysfunction, which can become persistent and decrease quality of life. Delayed testosterone treatment has been shown to reduce vestibular impairment and improve neuronal survival in the vestibular nucleus following rmTBI; however, the long-term molecular pathways associated with rmTBI and testosterone treatment remain widely unexplored. We utilized a 5-hit closed-head rodent rmTBI model and administered testosterone 35 days after injury (DPI). The vestibular nuclei were removed and prepared for RT-qPCR and western blotting. Gene expression and protein levels were elevated for markers of oxidative stress (NADPH oxidase 4) and …

Biofilms On Plastic Litter: Community Composition And Activity And The Effects On Ecosystem Processes, Raul F. Lazcano

Master's Theses

The rapid increase of plastic production and disposal has resulted in plastic pollution becoming a global problem. In aquatic ecosystems, plastic litter is a substrate for biofilms, but little research has simultaneously assessed the effects of plastic litter on biofilm activity, community composition, and ecosystem processes. Our objectives were to: (1) measure biofilm activity and community composition on plastic litter relative to a natural surface in an urban river and (2) assess the impact of microplastic pollution on ecosystem metabolism and N2 flux in an oligotrophic lake. For objective (1) we incubated three common plastics with distinct physical and chemical …

Dna Methylation Patterns Of Line-1 Elements Specific To Hc21p In Cancer Cells, Dametria Jetters

Master's Theses

Long Interspersed Nuclear Elements (LINEs) are retrotransposons. They function by making a cDNA copy, reverse-transcribing it into DNA and reinserting itself back into the genome; thereby, increasing its copy number. Sequencing of the human genome revealed that LINEs (L1) are underrepresented on the short arm of human chromosome 21 (HC21), but it is enriched in full-length copies. A number of previous studies have investigated the DNA methylation patterns of L1s in cancer cells, but these studies are typically done in euchromatic regions. The heterochromatic short arm of HC21 is primarily composed of heterochromatin. However these regions can be targeted for …

Unveiling Bacterial Hitchhikers In The Urobiome, Genevieve Baddoo

Master's Theses

Microbial communities of bacteria, viruses, and fungi form complex interactions in nature. However, our understanding of these communities is limited to those that can be cultured in the lab. Enhanced culture methods have enabled the isolation of numerous fastidious species from urinary tract, definitively proving that the urinary tract of asymptomatic individuals is not sterile. In working with these isolates, our group has observed several instances in which a “purified” isolate actually harbors more than one species. We refer to these additional members as bacterial hitchhikers, a term previously used to describe similar occurrences in soil communities. I hypothesized that …

Psychological Symptoms, Visceral Pain Sensitivity, And Observations Of Parent Pain Behavior Are Related To Somatic Symptoms In Premenarchal Adolescents, Genevieve Roth

Master's Theses

Somatic symptoms are a risk factor for the development of chronic pelvic pain in women. The present study characterized somatic symptoms and their relations with psychological, pain sensitivity, and parental factors in a community sample of premenarchal adolescents, which marks an initial step in advancing the identification of potential modifiable factors related to menstrual pain and chronic pain development. Pain sensitivity (visceral and bodily factors) and psychological symptoms (anxiety and stress) were predicted to be related to somatic symptoms (e.g, widespread bodily discomfort) in premenarchal adolescents. Additionally, adolescents’ observation of parent pain behavior was expected to be specifically associated with …

Temozolomide-Nsaid Mixtures And Novel Diclofenac-Purine Hybrids As Potential Antiglioblastoma Agents, Joshua Rotich

Master's Theses

Glioblastoma (GBM) is the most common, lethal and aggressive brain tumor in adults. Standard treatment involves surgery, radiation therapy and temozolomide (TMZ) chemotherapy. However, GBM recurs and the average survival rate is between 12 to 18 months with 25% 1-year survival rate and 9% 5 years survival rate. Treatment options and advancement is limited by the blood brain barrier (BBB) which restricts drug entry into the brain and the immense heterogeneity of the tumor which limit adequate control of the entire tumor using one drug. In this research, we explored whether a combination mixture of TMZ and non-steroidal anti-inflammatory drugs …

A Comparison Of Impa2 & Isyna1 Gene Expression And Intracelular Myo-Inositol Levels In Bipolar And Non-Bipolar Disorder Derived Human Lymphoblasts, Christina Rosette

Master's Theses

The thesis objective was to determine if myo-inositol concentration levels and the gene expression of IMPA2 & ISYNA1 differ among bipolar disorder types 1 and 2 compared to healthy controls (non-bipolar disorder). Previous studies have correlated different myo-inositol concentration levels with specific phases of bipolar and pharmaceutical treatments. Two genes, inositol monophosphatase 2 (IMPA2) and inositol-3-phosphate synthase 1 (ISYNA1), involved in de novo myo-inositol biosynthesis have been implicated in the pathophysiology of bipolar. In this study, differences in myo-inositol concentration and IMPA2 & ISYNA1 gene expression in lymphoblasts derived from subjects with bipolar types 1, 2, and healthy control were …

Peruvian Mammals Collected By The Andrews University. Department Of Biology : Expeditions & Patterns Of Diversity, Kieran M. Taylor

Master's Theses

The Department of Biology at Andrews University conducted three expeditions to Peru in 1964, 1965, and 1968. During the expeditions, thousands of specimens were collected of several taxa, including mammals. As far as we know, no complete summary of the expeditions and the mammals collected has been created. Knowledge of the expeditions and evaluation of the mammals collected compared to current literature could have conservation implications. My purpose was to recreate the story of the expeditions and use this contextual information to catalog and identify the number of mammal species collected and evaluate the mammal diversity collected according to current …

Negotiating Transportation Insecurity: Local Responses And Coping Strategies In San José, Ca, Andrew K. Ng

Master's Theses

Transportation insecurity causes adverse effects on people’s lives by limiting access to opportunities and resources even in San José. Understanding how people experience transportation insecurity in metropolitan areas may contribute to building a better transportation system. I conducted my research study by interviewing downtown San José residents and analyzing their stories on the effects of transportation insecurity and what they did to cope with the situation to move around San José. I analyzed the contents of the interviews through qualitative data analysis. Findings suggest that people experience transportation insecurity as time infringement through congested traffic, convoluted schedules, and service complications, …

Two-Level Data Augmentation With Transfer Learning For Classification Of Medical Images With Limited Data, Nihil Pudota

Master's Theses

Machine learning used in the medical industry can potentially detect cancer in humancells at an early stage. However, training the machine learning models, especially deep learning models require thousands to millions of samples in order to reach an acceptable accuracy level. It is well-know that obtaining medical data is tedious hence in most cases, medical datasets have limited number of data samples. One solution for this problem is utilizing transfer learning such as pretrained networks on another dataset. Another solution is to increase the number of training data points with data augmentation. Common data augmentation methods for images include not …

An Examination Of Factors Predicting Academic Engagement And Commitment Among Science, Technology, Engineering, And Mathematics Students: The Moderating Role Of Gender, Tasanee S. Thienpothong

Master's Theses

The purpose of the present study was to examine the role of gender on the relationships between the predictor variables (i.e., sense of belonging, grit, and support systems) and academic engagement and commitment to major among science, technology, engineering, and mathematics (STEM) students. Specifically, this study proposed that gender would act as a moderator of these relationships such that the relationships between the predictor variables and the outcome variables would be stronger for women than for men in STEM. A total of 254 undergraduates from a university in Northern California participated in an in-person survey. Although results did not show …

“It Made Me Question If I Was In The Right Hands”: Exploring Cancer Prognostic Conversations Between Providers And Latinx Patients, Yesenia Anabel Carrillo

Master's Theses

People of color are often confronted by barriers within the health-care system that white people are not likely to encounter. Latinx patients are among those who experience racial health disparities, typically due to the lack of health literacy support and limited resource access. Drawing on previous work that focuses on exploring patient, provider, and caregiver preferences for cancer prognostic conversations; this study explores perceptions of cancer prognostic conversations between providers and Latinx patients to better understand the degree of cultural competence among providers and opportunities for enhancing interpersonal communication with Latinx patients. Interviews with seven patients and five providers were …

Association Between Meal Dietary Inflammatory Potential And Postprandial Sleepiness, Samantha Beth Kalb

Master's Theses

The amount and composition of food that is consumed are known to contribute toinflammation and may affect how we feel in the following hours. The aim of this research was to determine if the inflammatory potential of a single meal is associated with level of postprandial sleepiness. Participants (n = 70) recorded intake of one of their meals, then reported their degree of postprandial sleepiness (Stanford Sleepiness Scale), average activity level, average hours of nightly sleep, and daytime sleepiness (Epworth Sleepiness Scale). The Dietary Inflammatory Index (DII) of the meal was estimated by adjusting DII parameters by 26%, reflecting the …

A Chemical And Minerological Fingerprint Of Hydraulic Mining Sediment, In The Yolo Bypass, California, Stephanie Annette Meursing

Master's Theses

Hydraulic gold mining in California, began not long after the discovery of gold in 1848. This type of mining dislodged large amounts of sediment, which were then deposited downstream. The Yolo Bypass, downstream from several northern California rivers, has received and continues to receive hydraulic mining sediment (HMS). The main goal of this study was to document the chemical and mineralogical characteristics of HMS in the bypass. Twelve borehole cores from the bypass and five in-situ auriferous gravel samples, which were used for background concentrations, were collected for this study. All twelve cores had higher Hg concentrations than the in-situ …

Hybridity And Habitation: A Rhetorical Analysis Of Interior Design In Live-Action Cyberpunk Films Through The Lens Of Posthumanism And Thing Theory, Sydney Ng

Master's Theses

Characterized by hyper-urban environments, extreme class division, and an abundance ofcorporate oversight, the cyberpunk subgenre of science-fiction is a prime candidate for scholarly research on speculative interior design and associated technologies. Using the theoretical frameworks of posthumanism, or the concept that humans will transcend their current biological form in the near future, and thing theory, or the worldview that objects are able to enact their autonomy on living subjects, interior design in three live-action films in the cyberpunk subgenre were analyzed in order to determine how depictions of future spaces reflect present ideations of our potential real future. Metaphor analysis …

Urban Edge Effects On Soil Conditions, Stand Structure, And Understory Composition In A Coast Redwood Forest, Nanako Oba

Master's Theses

Forests in the wildland-urban interface (WUI) can experience edge effects caused byhuman activities, such as timber harvest and urban development, altering vegetation composition and structure. Both the WUI and resulting edge effects are well studied in general; however, the influences of the urban edge on coast redwood forests specifically are not well understood. I analyzed soil properties, stand structure, and understory composition in a coast redwood preserve in the Santa Cruz Mountains, California using twenty 300 m transects established across an anthropogenic edge and twenty transects within a forest interior control. Spearman’s rank correlations indicated that several variables exhibited positive …

An Assessment Of The Diverse Freshwater Turtle Fauna Of Mississippi, Lucas Haralson

Master's Theses

Nestled within the Southeastern United States Turtle Priority Area, Mississippi boasts impressive freshwater turtle diversity and is home to 8.6% of extant turtle species. Despite this impressive richness, few studies have assessed freshwater turtle assemblages in this hotspot of aquatic diversity. This research aimed to elucidate the distributions and abundances of freshwater turtle species through a statewide investigation of all major river drainages of the state. Using baited hoop nets (90 cm and 120 cm diameter), turtles were collected, identified to species and sex, and measured, before being released. Turtle assemblages were assessed in both lotic and lentic environments from …

Risk Perception And Response Among International Students Of The University Of Southern Mississippi, Elida Lopes Souza Rocha

Master's Theses

Given the exposure of university campuses to hazards, disaster mitigation is a critical element of higher education policy. Although U.S. higher education institutions are leaders in the global education market, emergency warning systems give little consideration to how international students perceive risk, prepare for hazards, or access warning technologies available to them. This poses several questions regarding the suitability of hazards mitigation practices and the welfare of international students.

This thesis investigates the relationship between USM international students and natural hazards. Responses from online surveys and semi-structured interviews data were analyzed through qualitative and quantitative methods to document the extent …

Map-Gan: Unsupervised Learning Of Inverse Problems, Brandon S. Campanella

Master's Theses

In this paper we outline a novel method for training a generative adversarial network based denoising model from an exclusively corrupted and unpaired dataset of images. Our model can learn without clean data or corrupted image pairs, and instead only requires that the noise distribution is able to be expressed analytically and that the noise at each pixel is independent. We utilize maximum a posteriori estimation as the underlying solution framework, optimizing over the analytically expressed noise generating distribution as the likelihood and employ the GAN as the prior. We then evaluate our method on several popular datasets of varying …

Kaleidoscopic Katas: An Intercultural Somatic Curriculum For Holistic Health, Emily Seymour

Master's Theses

The intent of this research study was to combine three African and Eastern somatic movement practices into a modern dance curriculum. Few studies have investigated the therapeutic value of subtle body awareness in a dance class context. This integrative study combined psychological, physiological, and contemplative pedagogical approaches, which led to the development of a comprehensive five-part workshop with five adult participants. This ten-hour online class series involved learning a traditional Afro-Haitian serpent dance called the Yanvalou, the Tantric practice of Chakra Yoga, and the Five Animals qigong. The original curricular goal was to combine these three modalities into five sequential …

Balancing Increased Physical Demands: An Analysis Of Cross-Training Practices In Young Adult Ballet Dancers, Brooke Yuspeh

Master's Theses

The purpose of this study was to see what young adult ballet dancers knew about cross-training and if they found cross-training methods they practiced effective in supporting their dance technique and helping to prevent injuries. To uncover this information, the researcher used the following essential questions to guide this project: Q1: How do young adult ballet dancers practice cross-training? Q2: Which of these cross-training modalities are considered most effective? The research instruments used in this study included a survey consisting of multiple-choice, multiple-selection, and open-ended questions and an optional journal reflection consisting of open-ended questions. There were a total of …

A Multigrid Homotopy Method For Computing Eigenfunctions Of Differential Operators With Two-Dimensional Heterogeneity, Chelsea Drum

Master's Theses

In this thesis, we develop an accurate algorithm for computing the smallest eigenvalues of the self-adjoint spatial differential operator for the two-dimensional heat equation with a piecewise constant coefficient and periodic boundary conditions. The piecewise constant coefficient is created by the discontinuity of the diffusivity coefficient across the interfaces between two or more heterogeneous materials. Our method involves the combination of the well-known Inverse Iteration with a multigrid homotopy.