Digital Commons Network^™

Anode Material Testing For Marine Sediment Microbial Fuel Cells, Andrew John Quaid

Master's Theses

Research on the power production of the microbial fuel cell has increased in the past decade. The sediment microbial fuel cell is a type of fuel cell that uses the environment of submerged sediments to provide a natural voltage difference. The fuel cell is comprised of an anode buried in the sediment and a cathode that is held in the overlying water column. The process of electron transfer to the anode is catalyzed by anaerobic bacteria in the sediment. The anaerobic bacteria have that are able to catalyze the electron transfer have been termed exoelectrogenic. The increase in scientific research …

Panic Behind The Mask: The Spanish Influenza Epidemic Of 1918 In New Orleans, Sarah Theresa Savage

Master's Theses

As part of the most devastating influenza pandemic in modern history, the Spanish Influenza epidemic in New Orleans left the city emotionally and physically crippled as residents struggled to resume daily life after thousands succumbed to a bloody cough and painful death in October 1918. When New Orleans public health officials reacted to the explosion of Spanish Influenza cases on October 10, 1918, the virus had already traveled throughout the population. Unlike previous influenza outbreaks, the 1918 epidemic killed primarily young healthy adults, the backbones of the working force and families. In an attempt to quarantine the ill from the …

Evaluating Macrobenthic Indicators Of Organic Enrichment And Hypoxia Within The Coastal Mississippi Hypoxic Zone, Daneen P. Menke

Master's Theses

Macrobenthic communities offer effective indicators of biotic integrity, but their use for distinguishing anthropogenic from natural stress is tricky because coastal taxa are eurytolerant. Effective coastal management calls for benthic indicators that respond to specific stressors, apply across different habitats, and reflect ecosystem function. Macrobenthic process metrics based on body-size descriptors should reflect ecosystem function and be useful for assessing the effects of eutrophication. Functional trait analysis should reflect the functional diversity of the community. Coastal Mississippi experienced widespread and sustained hypoxia throughout summer 2008. Site 6 located on the 10-m isobath in the center of the 2008 hypoxic zone …

The Effects Of The Good Behavior Game With General Education High School Students, Rachel R. Mitchell

Master's Theses

The purpose of this study was to evaluate the effectiveness of the interdependent group contingency procedure known as the Good Behavior Game (GBG) on decreasing disruptive behaviors in general education high school students. The effectiveness of the GBG has been investigated in many studies as means of managing a variety of behaviors across many developmental levels; however, not all populations have been investigated. Although many studies exist that have utilized the GBG to alter behaviors across ages ranging from pre-school to adulthood, few studies exist in which the GBG has been used with a general education high school population. The …

Phase Speciation Of Carbohydrates And Dioxins In Rivers And Coastal Waters In The Northern Gulf Of Mexico, Kusumica Mitra

Master's Theses

Dissolved carbohydrates (d-CHO), including monosaccharide (MCHO) and polysaccharides (PCHO), and particulate carbohydrates (p-CHO) are major components of natural organic matter and play an important role in biogeochemical cycles of carbon and other trace elements in marine environments. This study aimed to examine the abundance and partitioning of CHO between dissolved and particulate phases and their seasonal variations in the Mississippi Sound/Bight and Lake Pontchartrain. Laboratory mixing experiments were also carried out to examine the behaviour of CHO species along a salinity gradient. The phase partitioning of dioxin among three different phases, i.e., colloidal organic matter, particulate organic matter and sediments, …

Bacterioplankton Production In The Western Mississippi Sound And The Role Of Photosynthetic Extracellular Release As A Driver Of Bacterial Production, Amy Michele Glover

Master's Theses

Bacterioplankton growth and metabolic activities are a vital component in structuring marine and estuarine ecosystems. In this study, bacterial production (BP) was investigated at two separate stations in the western Mississippi Sound (MS). One was an inshore site outside of the Bay of St. Louis and the other site was located in the transition zone (offshore) between the MS and Mississippi Bight, just south of Cat Island. Bacterial production (BP) was analyzed using the addition of ¹⁴C leucine to natural water samples. In addition, samples were augmented with algal exudates in order to investigate BP based on uptake of …

Improved Microalgal Biomass Harvesting Using Optimized Environmental Conditions And Bacterial Bioflocculants, Derek C. Manheim

Master's Theses

ABSTRACT

IMPROVED MICROALGAL BIOMASS HARVESTING USING OPTIMIZED ENVIRONMENTAL CONDITIONS AND BACTERIAL BIOFLOCCULANTS

DEREK CONTE MANHEIM

The cost and energy balance of microalgae biofuel production is sensitive to the algae harvesting method, among many other factors. Bioflocculation and settling of suspended microalgae cultures is a harvesting method with potentially low cost and energy input. However, bioflocculation (the spontaneous flocculation of algal cells without chemical addition) has not been a reliable process with cultures grown in ponds. To provide insights to help improve algae settling, factors affecting the settling of algae were investigated in the laboratory using pure cultures of two common …

In Vivo And In Vitro Digestibility Of A Complete Pelleted Feed In Horses, Cassandra Renee Sweeney

Master's Theses

ABSTRACT

COMPLETION OF AN IN VIVO DIGESTIBILITY TRIAL IN HORSES AND IN VITRO DIGESTIBILITY ASSAY DEVELOPMENT

Cassandra Renee Sweeney

In vivo analysis of equine feed digestibility has been the gold standard since the late 1800's, although it can be time consuming, costly, and labor intensive. In vitro digestibility analysis may be more economical and beneficial to both feed manufacturers and consumers. The availability of accurate in vivo data is crucial for critical evaluation and validation of any potential in vitro method (Coles et al., 2005). Ten adult American quarter horse geldings were used in the in vivo digestibility evaluation of …

Aloe Striata Plant Defense Compounds Produced In Response To Jasmonic Acid, Salicylic Acid, And Eriophyid Mite Salivary Extract Elicitor Compounds, Fritz Light

Master's Theses

Aloe mites are herbivores of the genus Aloe (ALOACEAE) and are associated with hyperplastic growth in various aloe species, but the biochemistry of this interaction is poorly understood. In an effort to characterize plant defense responses to herbivory in the genus Aloe, a salivary extract was isolated from aloe mites (Aceria aloinis Keifer) and its bioactivity was tested using a hypocotyl elongation assay. Subsequently, Aloe striata plants were treated with jasmonic acid (JA), salicylic acid (SA), and the mite salivary extract. Using water and methanol, compounds of different polarity were extracted from aloe tissues that had been frozen …

Synthesis Of Half-Loaded Nitroimidazole Indocyanine Green Dyes Attached To Carbon Nanotubes, Courtney L. Stanford

Master's Theses

Indocyanine green dye is the only FDA approved NIR fluorescent dye that can be used in humans. 2-Nitroimidazoles are very effective radiosensitizers, have low cytotoxicity, and target hypoxic tissue. By coupling the two together, hypoxic tumors can be targeted and imaged. Previous work has been done to couple 2-nitroimidazole to both sides of the indocyanine green dye. This work focuses on expanding that concept to couple 2-nitroimidazole to only one side of the dye, thus allowing for the other end to be available to couple to another compound, generating a half loaded 2-nitroimidazole indocyanine green dye. In theory, this half-loaded …

Dietary Protein Influences Paracellular Calcium Transport: Two Molecular Targets, Belinda M. Kotler

Master's Theses

The effect of dietary protein on bone health remains controversial. We have shown that increasing dietary protein increases calcium absorption in both humans and rats with no change in bone resorption. We used a rat model in which dietary protein increases intestinal calcium absorption and whole body calcium retention, to explore the molecular basis for this effect. Female Sprague-Dawley rats were fed a low (5%) or high (40%) protein diet for 7 days. On day 7, duodenal mucosa was harvested and total RNA isolated. Microarray analyses using these RNA samples identified 208 genes whose expression was at least 1.5 fold …

Fluorescence Tagging Of Marine Low Molecular Weight Carboxylic Acids, Brian C. Gismervik

Master's Theses

This thesis aims at the evaluation of the best amide bonding strategy that places a fluorescence aminomethyl coumarin tag onto a LMW organic acid in aqueous solution. A three-stage study was conducted. First, a suitable aminomethyl coumarin fluorophore was synthesized. Second, a library of acid-derivatized standards was synthesized for use in optimizing the anticipated HPLC separation of a mixture of LMW acids expected to be found in marine samples. Third, the aforementioned amide coupling strategies were screened to identify the most applicable method for our reaction conditions to the tagging of LMW organic acids in a large number of field …

"We've Got Something More To Prove Here": Racial-Ethnic And Social Class Identities And The Challenges Facing Latina/O College Students, Gretchen Marin

Master's Theses

Latinas/os are one of the fastest growing minority groups in the United States. In fact, the United States Census Bureau projects that by the year 2050, the numbers of Hispanics will more than double, increasing from the current figure of 50.5 million to 102.6 million. Despite such rapid population growth, the numbers of Latinas/os with at least a Bachelor’s degree remain low. In this study, I explore the intersection of racial-ethnic and social class identities in relation to academic identities. Specifically, I examine the challenges facing Latina/o college students of various socioeconomic backgrounds as they negotiate a predominantly White, middle-class …

A Qualitative Study Of Barriers To Dental Care Among Older Minority Adults Residing In Lower Income Communities, Demetress Davis

Master's Theses

ABSTRACT: The purpose of this study was to explore factors that presented barriers to the use of dental care among low-income minority older adults. Participants were recruited from low-income senior housing in Hartford, CT. In-depth semi-structured interviews were conducted to obtain data on demographics, oral health status, oral health knowledge and factors that affected use of dental care including finances, perceived oral symptoms, cultural factors, and beliefs about dental care. Interviews were conducted with 17 participants. The major perceived barriers were ability to pay for care, transportation and fear/mistrust of the dentist. Participants may have had poor oral health as …

Immediate Versus Delayed Loading Of Two Implants Supporting A Locator Retained Mandibular Overdenture. A Randomized Controlled Study., Sejal R. Thacker

Master's Theses

The aim of this study was to evaluate immediate versus delayed loading of two implants, six months post-surgery, supporting a locator retained mandibular overdenture. The objective was to evaluate implant bone remodelling using radiographic bone level changes (RBL) as the variable, from baseline to six months, between the test and the control groups. The secondary objectives included (1) evaluation of implant survival, (2) nature and frequency of surgical and prosthetic complications, and (3) correlation of implant length and insertion torque on RBL.

Eligible patients were randomized into test (immediate loading) and control group (delayed loading). Five patients were allocated to …

Tissue Engineering A Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance Testing, Navid Djassemi

Master's Theses

Tissue Engineering A Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance Testing

Navid Djassemi

Tissue engineering blood vessel mimics has been proposed as a method to analyze the endothelial cell response to intravascular devices that are used in today’s clinical settings for the treatment of cardiovascular disease. Thus, the development of in vitro blood vessel mimics (BVMs) in Cal Poly’s Tissue Engineering Lab has introduced the possibility of assessing the characteristics of cellular response to past, present, and future intravascular devices that aim at treating coronary artery disease.

This thesis aimed at improving the methods and procedures utilized in …

The Spatial Distribution Of K-Factor Values Across A Toposequence And A Soil Survey Map Unit, Jacqueline Elizabeth Tilligkeit

Master's Theses

Rivers and streams are adversely affected by an increase in sedimentation in their waters from eroding land. High sediment loads in streams can bury fish eggs and prevent hatching, increasing nutrients in the water causing algae blooms, or even contaminating the water with heavy metals carried in or on the aggregates. The erodibility of soil is valuable knowledge to all land users so that we may predict soil loss and its potential to pollute streams. This is done by using the Revised Universal Soil Loss Equation (RUSLE). By predicting soil loss from a given landscape, land managers can take mitigation …

Development Of In Vitro Tissue Engineered Blood Vessel Mimics In Complex Geometries For Coronary Stent Testing, Robert Dalton Chavez

Master's Theses

Coronary heart disease is the leading cause of death in the United States and occurs when plaque occludes coronary arteries. Coronary stents, which may be used to treat coronary occlusions, are small metal tubes that are implanted in coronary arteries to restore blood flow. After stent implantation, endothelial cells grow over the stent so that blood contacts the endothelial cells instead of the stent surface; this event is known as re-endothelialization. Re-endothelialization prevents blood from clotting on the stent surface and is a good predictor of stent success. Blood vessel mimics (BVMs) are in vitro tissue engineered models of human …

Characterization Of Tight Junction Formation In An In-Vitro Model Of The Blood-Brain Barrier, Michael Robert Machado

Master's Theses

Active and passive transport of substances between the microcirculation in the brain and the central nervous system is regulated by the Blood-Brain Barrier (BBB). This barrier allows for chronic and acute modulation of the CNS microenvironment, and protects the brain from potentially noxious compounds carried in the circulatory system. In-vitro modeling of the BBB has become the target of much research over the past decade, as there are many unanswered questions regarding modulations in the permeability of this barrier. Additionally, the development of a practical and inexpensive model of the BBB would facilitate a much more efficient drug development process. …

Development And Characterization Of An In-House Custom Bioreactor For The Cultivation Of A Tissue Engineered Blood-Brain Barrier, Amin Hadi Mirzaaghaeian

Master's Theses

The development of treatments for neurological disorders such as Alzheimer’s and Parkinson’s disease begins by understanding what these diseases affect and the consequences of further manifestation. One particular region where these diseases can produce substantial problems is the blood-brain barrier (BBB). The BBB is the selective diffusion barrier between the circulating blood and the brain. The barrier’s main function is to maintain CNS homeostasis and protect the brain from the extracellular environment. The progression of BBB research has advanced to the point where many have modeled the BBB in vitro with aims of further characterizing and testing the barrier. Particularly, …

Virtual Three-Dimensional Analysis Of Dimensional Accuracy Of Dental Master Die Models Created By The Iterotm Electronic Impression Device And Those Made From A Polyvinyl Siloxane Impression Material, Polina P. Shcherbatov

Master's Theses

No abstract provided.

The Effect Of Corticision On The Rate Of Orthodontic Tooth Movement In Rats, Hamed Vaziri

Master's Theses

Minimal treatment time is a primary goal for orthodontic therapy. Reducing treatment time decreases risk of caries, periodontal disease, root resorption, and increases patient satisfaction. Orthodontic tooth movement (OTM) is a result of bone resorption and apposition; factors influencing the rate at which these processes take place may affect OTM. Various attempts have been made to shorten treatment time. The regional acceleratory phenomenon (RAP) has been used to modify the balance between resorption and apposition of bone through selectively injuring the cortical plate of the alveolus to accelerate tooth movement. This approach is limited due to the need of flap …

Analysis Of Periapical Biopsies Submitted For Histopathological Evaluation: A Retrospective Study, Abdullah I. Alqaied

Master's Theses

Apical periodontitis is the most common pathological process in the periapical region. Disciplined clinical and radiographic evaluations and appropriate diagnostic tests can detect lesions related to apical periodontitis. Aside from this, lesions mimicking pulp-related pathology but unrelated to pulpal infection and necrosis are occasionally discovered in the periapical region. The objective of this study was two-fold: (1) to determine the prevalence of diverse periapically located pathological entities, and (2) to apply that information to evaluate the rationale for routine submission of surgically obtained tissue for histological examination and diagnosis. Methods: A 5-year retrospective analysis of pathology reports from the UCONN …

Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer

Master's Theses

This study was done to determine the effectiveness of a commercially available bioaugmentation product, BiOWiSH^TM-Aqua FOG, for remediating petroleum-contaminated sandy soil. Biodegradation enhancement by BiOWiSH^TM-Aqua FOG was evaluated in laboratory microcosms by directly measuring total petroleum hydrocarbon (TPH) and indirectly using respirometry. Attempts were made to enrich hydrocarbon-degrading bacteria in BiOWiSH^TM-Aqua FOG, and the resulting enrichment cultures were screened using respirometry as well. Potential hydrocarbon-degrading bacteria in BiOWiSH^TM-Aqua FOG were isolated. Experiments were performed at bench-scale using microcosm bottles containing sand contaminated with either motor oil or No. 2 diesel fuel. The …

Effect Of Oxidation On Weld Strengthof Dissimilar Resistance Weld Interface Between 304 Stainless Steeland Near Equiatomic Austenitic Nitinol Guide Wire, Matthew Rudow

Master's Theses

Abbott Vascular encountered strength and variability issues when attempting to resistively weld 304 Stainless Steel to equiatomic Nitinol. Initial observations suggested that passivation layer (Cr₂O₃, TiO₂) formation affected the weld interface. One hundred 304 Stainless Steel/Nitinol pairs were allowed to oxidize in air at room temperature for allowed periods of time (.1, 1, 3, 5, 7, 12, 16, 24, 168, and 336 hours). Each pair was welded resistively with constant current. A Miyachi/Unitek Advanced Data Analysis Monitor (ADAM) recorded the peak resistance at the instance the weld was made. Resistances were compared to Instron …

Viscoelastic Anisotropic Finite Element Mixture Model Of Articular Cartilage Using Viscoelastic Collagen Fibers And Validation With Stress Relaxation Data, Matthew Alexander Griebel

Master's Theses

Experimental results show that collagen fibers exhibit stress relaxation under tension and a highly anisotropic distribution. To further develop the earlier model of Stender [1], the collagen constituent was updated to reflect its intrinsic viscoelasticity and anisotropic distribution, and integrated with an existing mixture model with glycosaminoglycans and ground substance matrix. A two-term Prony series expansion of the quasi-linear viscoelastic model was chosen to model the viscoelastic properties of the collagen fibers. Material parameters were determined by using the simplex method to minimize the sum of squared errors between model results and experimental stress relaxation data of tissue in tension. …

Comparing Anti-Vegf Antibodies And Aptamers On Paper Microfluidic-Based Platforms, Katherine Noel Clayton

Master's Theses

The field of microfluidics is expanding into what is known as paper microfluidics. This uses a paper platform rather than materials (i.e. PDMS, PMMA) that are commonly used in microfluidics research. Current devices require an expensive manufacturing process and external sources to power the device. Such devices are not practical in low resource environments. As a consequence, it is the goal of this Thesis to develop a three-dimensional, multiplexed assay chip using nitrocellulose membranes. This device comprises of multiple layers of nitrocellulose membranes with defined fluidic channels. The multiple layers are bound together using double backed tape, and imbedded between …

Becoming Cittaslow: A City's Journey To Becoming A Cittaslow Member, Megan Alexis Elovich

Master's Theses

The project will explore Cittaslow as an alternative to traditional urban development. Sprawl and consumption of non-local resources are discouraged with Cittaslow and preservation of culture and history become the tangible benchmarks of the community. It will explore the history of Cittaslow as a movement and an organization; as well as its influences on existing member cities and the criteria used to distinguish them from others. The City of San Luis Obispo is used as a case study to determine whether existing conditions measure up to Cittaslow criteria.

Characterization Of Raw Milk Fouling On Plate-Type Heat Exchangers Using Different Alloys And Cow Phenotypes, Stephen Ernest Nelson

Master's Theses

ABSTRACT

Characterization Of Raw Milk Fouling On Plate-Type Heat Exchangers Using Different Alloys And Cow PhenotypeS

Stephen Ernest Nelson

Milk and other dairy products are widely used in many households today. Milk is a popular beverage that is seen as a healthy alternative to other synthetic beverages such as soda pop and other sugar based drinks. It became law that milk, and milk products are pasteurized before its release to the general public (FDA 2003). Pasteurization is a thermal process where the intent is to lower the concentration of microorganisms in the milk to render it safe to drink with …

A Conservation Plan For Reservoir Canyon Natural Reserve, San Luis Obispo, Ca, Brian M. Provenzale

Master's Theses

My thesis project is to create a conservation plan for the Reservoir Canyon Natural Reserve (RCNR) in San Luis Obispo, California. It is a professional project for the City of San Luis Obispo with the goal of eventual adoption by the City Council. The plan was motivated by City policy, which advises creating conservation plans for open spaces, and by a particular need to address management issues in RCNR that include plant and wildlife conservation, trail access, erosion, electrical utility easements, and other legal matters. The project consists of two main components: the conservation plan and a companion paper. The …