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The Impact Of Chronic Stress On Childhood Obesity And The Protective Effects Of Parental Warmth, Laura M. L. Distel

Master's Theses

Mexican American children have the highest rates of obesity in the U.S. Mexican American children may experience chronic stress, which has been linked to child obesity. Hair cortisol accumulation has been implicated as a mechanism for this association. Highly sensitive parenting may buffer the harmful effects of chronic stress and hair cortisol on obesity. Thus, the aims of this project were to 1) identify the direct and indirect effects of chronic stress and hair cortisol on children's zBMI and 2) examine the protective effects of parental warmth. This study examined hair cortisol levels, zBMI and chronic stress of children ages …

Coping With Exposure To Community Violence: Assessing The Role Of Avoidance In Reducing Delinquency And Aggression In Low-Income Urban Adolescents, Cara Marie Diclemente

Master's Theses

Exposure to community violence has disabling effects on the mental health of youth in the US, with high rates of exposure for African American adolescents from underserved, urban communities. Past literature suggests that avoidant coping, specifically behavioral avoidance, may be most useful for youth exposed to uncontrollable stress. The current study assessed the utility of coping strategies in reducing aggression and delinquency in 267 sixth through eighth grade African American youth. First, confirmatory factor analyses revealed a three-factor structure of coping: cognitive approach, behavioral approach, and avoidance. Next, moderated moderation findings showed that for boys, avoidance and approach strategies increased …

The Effects Of Treating Multispecies Biofilms With Bacteriophage, Alexandria Francis Cooper

Master's Theses

Biofilms can be found in any environment within proximity to water and are problematic in an assortment of industries. Numerous efforts have been employed to dislodge biofilms including bacteriophages (viruses that infect bacteria). Phage remediation is a promising solution for combatting biofilms that form on catheters in long term use patients. These biofilms often result in catheter-associated urinary tract infections (CAUTIs) and are the most common type of healthcare related infection reported. Additionally, they result in longer hospital stays and increased morbidity. To date, most of the research on the topic focuses on single species biofilms, despite their rarity in …

Potential Efficacy Of Targeting Mll1 In Breast Cancer, Austin Gable Holmes

Master's Theses

In 2017, it is estimated that breast cancer will be the most prevalent newly diagnosed cancer in females, at 30% in the U.S. alone1. From the early 1990s to 2014 the death rate in females from breast cancer has dropped by 10% but still stands as the second highest cancer related death in females in the U.S.2. One of the biggest hurdles in breast cancer research is disease heterogeneity. New mechanisms of disease development and progression are encountered frequently. One mechanism studied in the past decades is epigenetics. It has been theorized that the cancer epigenome can maintain an abnormal …

The Novel Characterization Of Extracellular Vesicles Containing Proteins Which Have Been Implicated In The Pathogenesis Of Hiv Associated Neurocognitive Disorders, Grace Ispas

Master's Theses

Although Anti-Retroviral Therapy (ART) is practiced, HIV-1 positive individuals still experience HIV-Associated Neurocognitive Disorders (HAND), collectively described as the presentation of neurocognitive, behavioral and motor dysfunctions that decrease the quality of life, while increasing the mortality in ART suppressed HIV-1 positive patients.

Current literature suggests that extracellular vesicles are involved in the pathogenesis of HAND as they are believed to be transferring HIV-1 proteins to nearby neuronal cells. Although most studies assume homogeneity among populations, characterizing cellular proteins or RNA levels in bulk, we hypothesize that distinct populations of extracellular vesicles are released and that environmental conditions, including viral infections, …

Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen

Master's Theses

Clean catch was developed before the advancement of more sensitive assays for culturing urinary bacteria. I reassessed clean catch, endeavoring to optimize this method. Periurethral swabs and voided urines were routinely collected, while analyzing different aspects of the method. I determined that midstream urine contained the least amount of vulvo-vaginal contamination and that the time of collection did not impact the results. I also determined that the use of antiseptic wipes prior to void increased the level of vulvo-vaginal contamination. A small cohort of females collected daily urines over a 17-day period and kept a strict alcohol diary. Females who …

Functional Studies Of Novel Mosquito Stage-Specific Genes In The Malaria Parasite, Plasmodium Berghei., Kaitlyn Kiernan

Master's Theses

The development and survival of the malaria parasite Plasmodium within its mosquito vector is not very well understood. When the parasite is taken up by an Anopheles mosquito, it is exposed to digestive enzymes, reactive oxygen species, increasing pH, decreasing temperature, and a diverse microbial flora. Our lab is studying the adaptive mechanisms underlying parasite survival in this hostile environment, one of which is movement out of the midgut. In order to escape the midgut, the parasite develops into an elongated, motile form called the ookinete. I am investigating two genes, thioredoxin-like protein 1 (trxl-1) and subpellicular microtubule protein 1 …

Comparing The Risk Factors Of Recidivism For Offenders With And Without Mental Illness, Meghan J. Mahoney

Master's Theses

This study examined which risk factors were predictive of recidivism among inmates released from Illinois Department of Corrections (IDOC) who had no mental illness, a non-substance abusing mental illness (non-SUD MI), a substance use disorder (SUD), or a co-occurring mental illness and substance use disorder (COD). The predictors of being returned to prison or being rearrested for a violent, property, or drug crime were compared across these four groups. A secondary data analysis was conducted on data obtained by Olson, Stalans, and Escobar (2016) for a study examining the predictors of recidivism for inmates released from IDOC in 2007. Logistic …

Comparative Studies On Biochemical And Pharmacological Profiles Of Bovine, Ovine And Porcine Heparins, Ahmed Kouta

Master's Theses

Heparin, a highly sulfated glycosaminoglycan (GAG), is used extensively as an anticoagulant. It consists of repeating disaccharide units, containing iduronic acid (or glucuronic acid) and glucosamine, exhibiting variable degrees of sulfation. Heparin, and its analogues, are used during surgery and dialysis, and are often used to coat indwelling catheters and other devices where the vascular system is exposed. Administered parenterally, often continuously due to its short half-life, over 0.5 billion doses are required per year. Heparin is currently obtained from mucosal tissue of meat animals, mainly porcine intestine, and to a lesser extent bovine and ovine sources. The early stages …

Developing Integral Ecologists: Description And Analysis Of The State Of Environmental Education At Jesuit High Schools In Light Of Religious Ecological Commitments And Science Education Research, Philip Nahlik Nahlik

Master's Theses

This research responds to numerous calls to focus more immediately and deeply on environmental education in the context of local communities. Jesuit high schools offer a rich network to study this movement, as schools with long histories of scholarly and moral concerns for forming students to think about and act on worldwide problems. The key research question is: How can science education research inform environmental education in these schools? The research methodology includes data collection and analysis of publicly-accessible website data, phone interviews with teachers, and a literature review of science education research. Recommendations are given for vertically aligning the …

Multifunctional Regulatory T Cells From Human Umbilical Cord Blood And The Role Of Tumor Necrosis Factor In Immune Homeostasis, Alexander Nelson

Master's Theses

Regulatory T cells (Tregs) are required to suppress inflammation and prevent autoimmunity. During fetal development Tregs are crucial to maintain tolerance between mother and child. After birth, neonates require tolerance to avoid harmful immune responses to foreign antigens in food and allow colonization with commensal microbes. We demonstrate a propensity for T cells in human umbilical cord blood to differentiate into Tregs in response to antigen receptor stimulation ex vivo. Cord blood-derived Tregs potently suppress T cell proliferation, but also produce pro-inflammatory cytokines known to activate innate immune responses. These results suggest that antigen exposure during early life results in …

Domestic Violence Among Asian Indian Immigrant Women In The United States, Teuta Peja

Master's Theses

This study looks at how domestic violence is conceptualized by the research studies on

Asian Indian immigrant communities in the United States through different periods of time, and

how sociocultural factors are associated with domestic violence. Using the meta-synthesis

descriptive method, I analyzed and interpreted the findings of four research studies conducted

between 1995-2016 that included in-depth interviews with Asian Indian immigrant women who

experienced domestic violence. The analysis focuses on changes of the patterns of abuse, helping behavior, and leaving the abusive marriage as an option. The changes in U.S. domestic

violence legislation and policy regarding immigration and domestic …

Chronic Effects Of Lead Exposure On Atherinops Affinis (Topsmelt): Influence Of Salinity And Organism Age, Erik Joseph Reynolds

Master's Theses

Pb (lead) appears in the environment as a consequence of both natural and anthropogenic processes. Mining, smelting, coal burning, lead acid batteries, and cement manufacturing substantially release Pb into aquatic environments. The goal of this study was to determine the influence of salinity and organism age on chronic toxicity of Pb to Atherinops affinis (topsmelt) in support of development of a species sensitivity distribution (SSD). Species sensitivity distributions assist in ecological risk assessments and establishing quality criteria for contaminants. Three chronic exposure studies were conducted for 28 days in a water flow-through testing system. Survival, standard length, dry weight, and …

A Mobile App Illustrating Sensory Neural Coding Through An Efficient Coding Of Collected Images And Sounds, Xiaolu Zhao

Master's Theses

Sensory neuroscience in the early auditory and visual systems appears distinct not

only to outside observers, but to many trained neuroscientists as well. However, to a computational neuroscientist, both sensory systems represent an efficient neural coding of information. In fact, on a computational level it appears the brain is using the same processing strategy for both senses - the same algorithm with just a change in inputs. Insights like this can greatly simplify our understanding of the brain, but require a significant computational background to fully appreciate. How can such illuminating results of computational neuroscience be made more accessible to …

West Indian Manatee (Trichechus Manatus) Habitat Characterization Using Side-Scan Sonar, Mindy J. Mclarty

Master's Theses

In this study, the reliability of low cost side-scan sonar to accurately identify soft substrates such as grass and mud was tested. Benthic substrates can be hard to classify from the surface, necessitating an alternative survey approach. A total area of 11.5 km² was surveyed with the sonar in a large, brackish mangrove lagoon system. Individual points were ground-truthed for comparison with the sonar recordings to provide a measure of accuracy. Five substrate types were identified: Dense seagrass, sparse seagrass, mangrove soil, mangrove soil with rock, and silt. A zoned benthic substrate map was created from the sonar recordings. …

Evaluating Contradicting And Confirming Evidence: A Study On Beliefs And Motivated Reasoning, Zachary Alan Caddick

Master's Theses

The purpose of this study is to examine ideological, psychological, and demographic predictors of motivated reasoning. Three-hundred and seventy-seven participants from Amazon’s Mechanical Turk system completed written responses critically evaluating strengths and weaknesses in a vignette on the topic of anthropogenic climate change. The vignette has two fictional scientists present prototypical arguments for and against anthropogenic climate change that are constructed with equally flawed and conflicting reasoning from both view points. Written responses were coded by a team of trained and reliability assessed qualitative raters. Motivated reasoning is operationalized by providing supporting evidence for the congruent belief and counter evidence …

Variation In Organismic Rna And Dna Content: Analysis And Application To The Assessment Of Living Planktonic Biomass, Elizabeth Lam Gagneron

Master's Theses

Modern analysis of DNA and RNA nucleic acid sequences has yielded profound changes in our understanding of the genetic biodiversity of planktonic organisms within the microbial food web of aquatic ecosystems. However, the bulk environmental concentrations of DNA and RNA, and their relative ratios, also potentially provide important information on the biomass and metabolic activity of planktonic organisms. Currently, there is a need to quantify the relative living biomass levels of natural water contained in ships’ ballast tanks to regulate the spread of aquatic invasive species (AIS) resulting from ballast water discharge practices within the international shipping industry. Ultraviolet (UV) …

Elucidating The Role Of Mastermind-Like 1 In The Degradation Of Myocyte Enhancer Factor 2c, Azia S. Evans

Master's Theses

Mastermind-like 1 (MAML1) is well characterized for its involvement in the Notch signaling pathway where it functions as a transcriptional co-activator and facilitates the degradation of the Notch intracellular domain (NICD). MAML1 has recently been implicated as a multifunctional protein that interacts with a variety of other signaling pathways, including Wnt-β-catenin, NF-B, and MEF2C (myocyte enhancer factor 2C). MEF2C is a member of the family of transcription factors involved in muscle cell differentiation and proliferation. MAML1 has previously been shown to activate MEF2C transcription. Given that MAML1 is responsible for degradation of the NICD, we wanted to know if MAML1 …

“A (Blind) Woman's Place Is (Teaching) In The Home”: The Life Of Kate Foley, 1873-1940, Angela Gates

Master's Theses

This thesis examines the life and career of Kate Foley, home teacher of the blind with the California State Library from 1914-1940. The purpose of this investigation is to determine how Foley, who was disabled, built a successful career with the state library despite facing significant discrimination and prejudice. Using a wide variety of primary source material, including letters, library publications, conference proceedings, newspaper articles, and census data, this biography evaluates Foley’s pioneering role as well as the challenges she faced. Home teaching provided a new vocational opportunity for blind women, whose professional choices were extremely limited. Despite her unique …

Sex-Specific Diet And Rockfish Consumption In California Sea Lions (Zalophus Californianus): Insights From Molecular Scatology, Keith Michael Hernandez

Master's Theses

Molecular diet analysis has the potential to overcome the limitations of traditional methods. I used prey hard parts and molecular methods to examine sex-specific diet trends and rockfish consumption of California sea lions (Zalophus californianus, CSL). Fresh scat samples (n=219) were collected from Año Nuevo Island, CA (ANI), during the summers of 2013 and 2014. Prey taxa were identified from fish otoliths and cephalopod beaks recovered from cleaned scats. Sex of the CSL depositing the scat was assigned via multiplex PCR of a CSL microsatellite and a carnivore Y chromosome marker. Prey species also were identified using multiple loci in …

Rna-Protein Structure Classifiers Incorporated Into Second-Generation Statistical Potentials, Takayuki Kimura

Master's Theses

Computational modeling of RNA-protein interactions remains an important endeavor. However, exclusively all-atom approaches that model RNA-protein interactions via molecular dynamics are often problematic in their application. One possible alternative is the implementation of hierarchical approaches, first efficiently exploring configurational space with a coarse-grained representation of the RNA and protein. Subsequently, the lowest energy set of such coarse-grained models can be used as scaffolds for all-atom placements, a standard method in modeling protein 3D-structure. However, the coarse-grained modeling likely will require improved ribonucleotide-amino acid potentials as applied to coarse-grained structures. As a first step we downloaded 1,345 PDB files and clustered …

Estuarine Habitat Use By The California Sea Otter (Enhydra Lutris Nereis), Jacqueline Kimberly Lindsey

Master's Theses

As the southern sea otter (Enhydra lutris nereis) range expands into northern and southern California, it will encounter estuaries that have been historically occupied by sea otters. Understanding how otters use re-colonized estuarine environments will inform how estuaries might be managed to encourage future sea otter range expansion. This project addressed the question: how do southern sea otters use space in the unique estuarine habitats of Elkhorn Slough? I compared the locations and behaviors of 25 individual sea otters of different status (male, female, and female with pup) among eelgrass, saltmud, saltmarsh, tidal creek, and main channel habitats in Elkhorn …

Sers Intensity Correlations To Lspr On Aggregated Au@Ag Systems, Caesar Alexander Munera

Master's Theses

The optimal surface enhanced Raman scattering (SERS) intensity was correlated to the localized surface plasmon resonance (LSPR) of individual and aggregated gold core/silver shell (Au@Ag) nanoparticles (NPs) in titrations involving the addition of both SERS label (e.g., rhodamine 6G, R6G) and the non-SERS active aggregant (chemical species that triggers the aggregation of NPs) potassium chloride (KCl). Titrating NP solutions with pure SERS label has often resulted in highly non-linear calibrations. In some cases, addition of non-SERS active aggregating agents such as KCl has also resulted in a large increase in SERS signals. An order of initial addition was followed in …

Integrative Analysis Of Heterogeneous Genomics Data For Triple Negative Breast Cancer And High Grade Serous Ovarian Cancer, Abdelrahman Hosny Ibrahim

Master's Theses

The human body is made up of trillions of cells. Although all the human body cells contain the same DNA sequence inside their nuclei, each one carries out its own function. Normally, human cells grow and divide to form daughter cells as the body needs them. When cells grow old, or lose their ability to function properly, they die (in a very organized way called apoptosis or programmed cell death) and new cells take their role. Cancer is a disease that is caused by uncontrolled division of abnormal cells in some part of the body, breaking the natural process of …

Azeotrope Enabled Anionic Polymerization Of Ethylene Oxide Using Initiators With Different Functional Groups, Dronareddy Madugula

Master's Theses

Poly(ethylene oxide) (PEO) is a nonionic hydrophilic polymer having the same repeat unit as poly(ethylene glycol) (PEG). It is of interest in both biology and materials science. PEO surfaces demonstrate a unique lack of protein adhesion, and PEO block copolymers are widely used in applications such as drug delivery. Unfortunately, the synthesis of PEO homopolymers and block copolymers can be experimentally challenging, with a typical method involving air sensitive organometallics and the removal of compounds such as naphthalene from the final product. Here we report a synthetic route that avoids these difficulties by using azeotropic distillation to remove water from …

The Symbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study And Taxonomic History, Nikolaus Schultz

Master's Theses

The green alga Oophila ambystomatus (Chlamydomonadales, Chlorophyceae) is

well known as the symbiotic partner of Ambystoma maculatum, the yellow-spotted

salamander. Recent molecular work has revealed phylogenetic diversity within isolates of

Oophila from New England. Here I investigate physiological properties and genetic

diversity of the algal symbionts representing different evolutionary lineages. In other

well-studied symbiotic systems involving algae, e.g., Chlorella and Hydra, algae and

lichen, or Symbiodinium and Scleractinian corals, the algal symbiont is capable of

multiple trophic modes, thus influencing its capacity as a partner. In this study I

investigated whether genetically differentiated strains of Oophila are capable of

absorbing …

The Politics Of Contagion, Joseph M. Young

Master's Theses

Contagion events have occurred throughout history leaving death and destruction in their wake. Often sensationalized in movies and shows such as Contagion and The Walking Dead, contagion events are life-altering events filled with gory symptoms and elevated mortality rates. The dangers of contagion events prompted governments to develop agencies with the purpose of preventing and mitigating the risks contagions pose. The Centers for Disease Control and Prevention (CDC) and other agencies across the globe pour millions of dollars each year into eliminating the risk of contagions and making the world a safer place. However, while more resources are dedicated to …

Distorting The Life Of Maca (Lepidium Miyenii), Fabiola Tavui

Master's Theses

The supply and demand of maca (Lepidium Miyenii), a native Peruvian vegetable, increased over the past decade; however, it has recently reduced. There are many implications for this outcome. Maca, a product with several health benefits, caught the attention of not just foreign businesses and investors but also of Peruvians. As maca became highly commercialized and exported abroad, the actors involved in the maca “business” at all levels contributed to its market to prospered but also to crumble. Some of the reasons due to the exploitation of maca itself for economic gains; distorting maca’s ecological environment and relation …

Rape As A Weapon Of War In Darfur, Fardowsa Abdullahi

Master's Theses

In the Darfur conflict the Sudanese government forces and the Janjaweed have used rape as a weapon of war, in the ethnic cleansing of non-Arab tribes in the region. Rape as a weapon of war was also used to degrade, terrorize women and destroy families and communities. In Darfur women and girls were raped in front of their family members. Victims ranging as young as eight years were raped in Darfur. This research investigates rape as a weapon of war. I took a qualitative approach by examining how rape was used as a weapon of war in Darfur and by …

Phylogenetics, Biogeography, And Climate Niche Variation Of South Pacific And Hawaiian Psychotria, Elaine Zhang

Master's Theses

Why do some species have broad geographic distributions, while other species are confined to a narrow distribution? Species age, ecological niche, or dispersal traits may help explain why some insular species are abundant and found on many islands, while others are rare and restricted to one island. In this study, I inferred a robust, time-calibrated phylogeny of the Hawaiian Psychotria, using two nuclear and eight chloroplast loci, sampling 67 individuals. I coupled my phylogenetic hypothesis with climatic data, ecological niche modeling, and morphological dispersal characteristics to explain the variation in number of islands occupied by each species. My inferred phylogeny …