Digital Commons Network^™

From La Costa Chica To Pasadena: Transnational Racial Politics Of Afro-Mexicans, Antonio Rodriguez-Santiago

Master's Theses

The ethnographic research in this thesis focuses on one group of Afro-Mexicans who migrated to Pasadena, California, from Mexico’s Costa Chica, on the Pacific Coast of Oaxaca and Guerrero, the area where a social movement for Afro-Mexican recognition and collective rights was founded in 1997. This study, using a framework of racial theory, examines the process of racial formation in a transnational setting, which has made this group different in many ways from the Black residents who remain in the Costa Chica. The study also considers how mestizaje, the Mexican national racial ideology that endorses a mixed-race society while …

Electropolymerization Of Polyaniline In The Presence Of Ferricyanide: Electrochemical, Spectroscopic And Gravimetric Studies, Lucas Marinelli

Master's Theses

In this work, ferricyanide-doped polyaniline (PANI) is electropolymerized by constant current and cyclic voltammetry (CV) measurements with simultaneous monitoring of mass changes using electrochemical quartz crystal microbalance (eQCM). Raman spectroscopy, Fourier transform infrared spectroscopy and CV measurements confirm the incorporation of the ferricyanide into the film and indicate modified electrical conductivity. UV-Vis absorbance spectroscopy and Raman spectroscopy confirm the conducting ‘emeraldine-salt’ state and confirm the presence of polarons, i.e. charge carriers.

The addition of ferricyanide during electropolymerization of the PANI film results in an increase of polymerization rate as confirmed using eQCM. A decrease in counter ion ingress/egress and an …

Afterschool Educators' Teaching Practices Through Tinkering: Nurturing Student Collaboration, Engagement, And Development Of Self-Confidence, Lianna Kali

Master's Theses

Since the early 2000s constructionist pedagogy has become an increasingly popular trend in both formal and informal science, technology, engineering, art, and math (STEAM) learning environments to support transformative educational outcomes through the making of personally meaningful objects and artifacts. But with this rise in popularity comes a challenge for educators: understanding what are the teaching practices that are most effective in a maker-centered learning environment to support student learning. Through an ethnographic case study of the teaching practices in a tinkering afterschool program, this study examines the core pedagogical facilitation moves of tinkering educators and investigates how those moves …

Detection And Melting Of Surface-Bound Dna Using A Purely Electrochemical Approach, Denny Ho

Master's Theses

In this thesis, we demonstrate an electrochemical approach for monitoring the electric-field induced melting of surface-bound double-stranded DNA. The electrochemical routine involves repeated chronoamperometry pulses to melt the duplex and square wave voltammetry to monitor the extent of melting. We utilize a scanning potential and constant potential technique to generate melting curves and access the stability and kinetics of the DNA duplexes. Our method uses a mixed monolayer of thiol-modified DNA oligomers and mercaptohexanol on gold electrodes, are subsequently incubated with target DNA covalently modified with electroactive methylene blue. Under room temperature, 10 mM Tris, and optimized electrochemical parameters, DNA …

The Best Interests Of The Child Or The State? The Rights Of The Child In Non-Lpr Cancellation Of Removal, Lizzie Bird

Master's Theses

This thesis argues that the United States is failing to fulfill its obligations under the Convention on the Rights of the Child (CRC) in its adjudication of the hardship standard in non-LPR cancellation of removal. It is well-documented that the current interpretation of the “exceptional and extremely unusual hardship” standard results in the separation of families and de facto deportation of children, many of whom are U.S. citizens. This thesis contends that this practice is not only unjust, but also unlawful.

First, it argues that the CRC in general and Article 3 (the “best interests” principle) in particular have risen …

Catalog Of Voluntary Carbon Offsets In Commercial Aviation: Understanding Roles Of Corporations And States, Shannon Hahn

Master's Theses

While the need for travel exists in the field of International Studies and in light of the very complex global climate crisis and increased international commercial air travel this paper catalogs and analyzes the trailblazing voluntary carbon offset programs of 1) Air New Zealand, 2) British Airways (IAG), 3) Cathay Pacific, 4) Emirates, 5) Lufthansa, 6) Qantas, 7) Scandinavian Airlines (SAS) and 8) United Airlines, arguing that voluntary carbon offsets as a response to the global climate crisis are ineffective and an echo to international agreements being unenforceable. The paper is hopeful for a corporate focus on technology and innovation …

Potential Impacts Of The Purple Salt Marsh Crab, Sesarma Reticulatum, On Long Island Sound Salt Marshes, Jaymie Frederick

Master's Theses

Salt marshes are important, highly productive ecosystems that provide many ecological benefits, including shoreline protection. Salt marshes were long believed to be driven by bottom up forces, however, recent research has suggested that top down influences may impact the foundation species Spartina alterniflora, and consequently marsh structure. This study looked at patterns of Spartina alterniflora dieback along creek bank areas and assessed Sesarma reticulatum abundance spatially throughout the marsh to improve understanding as to whether Sesarma may be influencing the dieback phenomenon. Dieback was occurring in all three salt marshes studied, at two of those marsh sites the rate of …

Using Personality Traits To Predict Pectoral Fin Contact Initiation Role In Bottlenose Dolphins (Tursiops Truncatus), Riley Paige Macgregor

Master's Theses

Contact with the pectoral fin facilitates formation and maintenance of social relationships between dolphins (Dudzinski & Ribic, 2017). Additionally, several studies have shown that bottlenose dolphins have distinct personalities that are consistent across time and situation (e.g., Highfill & Kuczaj, 2007; Kuczaj, Highfill, & Byerly, 2012), and it has been suggested that these individual differences (i.e., personality) may influence tactile behavior exchanges. The current study therefore aimed to determine if bottlenose dolphin personality traits predict whether and how dolphins initiate contact as a rubber or rubbee during pectoral fin contact exchanges, and to identify whether the effects of personality traits …

Ecological Stoichiometry: What Role Does It Play In The Competition And Spatial Distribution Patterns Of Aedes Albopictus And Aedes Aegypti?, James Deerman

Master's Theses

Ecological stoichiometry is the balance of chemical substances within animal bodies through interactions and processes within their ecosystem. Though relatively underexplored, it provides a wealth of information linking interactions across different levels of organization. Detritus is the base of the food web within the small aquatic ecosystems occupied by the mosquitoes Aedes albopictus and Aedes aegypti. Nutrient content of detritus varies, but it can have a negative effect on mosquito growth and survival if nutrient thresholds are not met. I investigated nutrient environments and species abundance in cemetery vases in New Orleans, LA to assess detrital heterogeneity and its …

Engineering Yeast To Evaluate Human Proteins Involved In Selective Rna Packaging During Hiv Particle Production, Ryan M. Bitter

Master's Theses

Despite recent advances in antiretroviral therapy, nearly 37 million people continue to live with human immunodeficiency virus (HIV). Basic and applied research on the assembly of HIV could be enhanced by using a genetically tractable organism, such as yeast, rather than mammalian cells. While previous studies showed that expression of the HIV Gag polyprotein in Saccharomyces cerevisiae spheroplasts resulted in the production of virus-like particles (VLPs), many questions regarding the utility of yeast in HIV assembly remain uninvestigated. Here, we report use of S. cerevisiae for both the production of VLPs with selectively packaged RNA and to evaluate the human …

Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land

Master's Theses

As keeping backyard poultry rises, human contact with zoonotic pathogens will increase. One such pathogen that backyard enthusiasts have exposure risks to is Salmonella spp. which may cause a potential public health threat due to its increasing multidrug resistancy. Salmonella spp. were present in 33 of 50 samples collected from 29 sites with backyard poultry coops in San Luis Obispo County during March to May in 2014. Two different Hardy-CHROME™ Salmonella Selective Media plates were used to culture and isolate positive samples of Salmonella spp.. Each positive isolate was tested for antimicrobial sensitivity to 6 standard antibiotics: Ampicillin, Bacitracin, …

Investigating The Effect Of An Upstream Spheroid On Tandem Hydrofoils, Joel Tynan Guerra

Master's Theses

This thesis documents a series of three dimensional unsteady Reynolds Averaged Navier-Stokes CFD simulations used to investigate the influence of an upstream prolate spheroid body on tandem pitching hydrofoils. The model is validated by performing separate CFD simulations on the body and pitching hydrofoils and comparing results to existing experimental data. The simulations were run for a range of Strouhal numbers (0.2-0.5) and phase differences (0-π). Results were compared to identical simulations without an upstream body to determine how the body affects thrust generation and the unsteady flow field.

The combined time-averaged thrust increases with Strouhal number, and is highest …

Characterizing Ecological And Genetic Patterns Of The Colonizing Species Dubautia Scabra On The Big Island, Hawaii, Samantha Kaleigh Naibauer

Master's Theses

The Hawaiian Silversword Alliance (HSA) is a group of approximately 35 endemic species found on the Hawaiian Islands. The HSA is of particular interest due to the vast adaptive responses of its members across the Hawaiian Islands. Each of these plant species is generally limited to a very specific habitat type with a unique morphology and physiology. Dubautia scabra is the first angiosperm colonist of new lava flows, arriving in as few as two years after lava cools. The critical role D. scabra plays in this ecosystem gives a unique starting point for understanding downstream ecological processes such as seed …

An Investigation Of Enzymes Capable Of Activating Cannabinoid Prodrugs, Brittney J. Morgan

Master's Theses

The plant Cannabis sativa contains a class of terpenophenols called cannabinoids which, in their active form, have been shown to have antiproliferative, anti-metastatic, anti-angiogenic, and pro-apoptotic effects on several types of cancer cells both in vitro and in vivo. Cannabinoids have been shown to not exert this apoptotic activity in healthy cells and they do not elicit the usual side effects seen with conventional chemotherapies. However, naturally occurring prodrugs from Cannabis must first be converted into the active form to induce the apoptotic and other physiological activities. Although humans do not possess the enzyme(s) necessary for this conversion, several different …

Venom Composition Of Little Known Mountain Rattlesnakes And Predator-Prey Interactions Of Crotalus Pricei Pricei And Its Natural Prey, Sceloporus Jarrovii, Emily Grabowsky

Master's Theses

The Crotalus intermedius clade is a small group composed of four species: C. intermedius, C. transversus, C. pricei, and the recently identified C. tancitarensis (Alvarado-Díaz and Campbell, 2004; Reyes-Valesco et al. 2013). Though these species are restricted to high elevations of Sky Islands of southern Arizona and throughout México, little has been reported about their natural history and basic biology, including venom composition. Specifically, the Western Twin-spotted Rattlesnake (C. pricei pricei) is a small lizard specialist restricted to the more northern Sky Islands of México, with isolated populations in southern Arizona, where they are a protected species. …

The Effect Of Spill Contaminants On Marine Biofilms And Microbially-Induced Corrosion Of Carbon Steel, Rachel Mugge

Master's Theses

There are over 2,000 known historic shipwrecks located in the northern Gulf of Mexico which are ecological and cultural resources that support the diversity of life found in the deep-sea when they become artificial reefs. Spilled crude oil and chemical dispersant from the Deepwater Horizon (DWH) spill may have impacted the preservation of deep-sea historic shipwrecks and their microbiomes if they are located in areas where oil and dispersant were deposited on the seafloor. Oil potentially accelerates corrosion of metal through a biologically-mediated process called microbially-induced corrosion (MIC). Introduction of spill contaminants may accelerate MIC on shipwreck hulls, which may …

Influence Of Natural And Anthropogenic Environmental Variability On Larval Fish Diet, Growth, And Condition In The Northcentral Gulf Of Mexico, Angie Hoover

Master's Theses

The northern Gulf of Mexico experiences high levels of freshwater runoff annually from various sources including the Mississippi River and Mobile Bay, among other sources. Early life history stages of fishes are especially vulnerable to environmental variability created by freshwater discharge. The objectives of this study were to describe the available prey field, diet, growth and condition of larval fishes with respect to various effects of freshwater discharge in the northern Gulf. The first chapter compared these parameters in larval Gulf Menhaden (Brevoortia patronus) collected from three different water masses characterized by physical and biological parameters after the …

Antioxidant Genes In Apis Mellifera, Their Implication In Pesticide Detoxification, Nicholas Rinderer

Master's Theses

The effect of pesticides on Apis mellifera mortality as well as their effect on the transcriptional regulation of antioxidant genes was the aim of this study. Unlike previous studies, the work here shows the combined effects of pesticides imidacloprid and coumaphos on worker bees at levels likely encountered by workers using both toxological and molecular analysis. Bee brood were collected from hives and incubated until hatching. The 1 day old bees were then segregated and subjected to varying concentrations of coumaphos and imidacloprid both independently and in concert. Workers from each treatment group were removed and stored in RNA-later until …

Epic Adolescence: Contemporary Adolescence In Philip Pullman’S His Dark Materials, Chloe Felterman

Master's Theses

To find the truth of a societal construct or phenomena, it can help to look at the world of fiction and fantasy. Though this idea may seem ironic or counter-intuitive, one will find that fictional literature can reveal the working order of its respective society. Philip Pullman’s epic fantasy trilogy, His Dark Materials, uses and manipulates the traditional constructs of the genre to reflect and re-imagine the concepts of adolescence of the late-20^th and early-21^st centuries. Eleven-year-old protagonist Lyra Belacqua and subsequently her cohort, Will Perry, reveal the complications and difficulties modern American and British adolescents experience as …

Freshwater Invertebrate Assimilation Nutrients From Detrital Biofilms Under Light And Phosphorus Manipulation, Taylor Price

Master's Theses

Anthropogenic influences are increasing algal biomass and activity within aquatic ecosystems, stimulating debate regarding interactions between “green” and “brown” food webs. Specifically, in forested streams, the removal of forest canopy or riparian zones increases light exposure and nutrient fluxes to streams affecting primary productivity and carbon (C) flows across aquatic food webs. Further understanding the synergistic effects of light and nutrients on detrital biofilms may explain how aquatic detritivores respond to anthropogenic change. The objective of this study was to employ C and phosphorus (P) isotopic radiolabels to understand how microbial biofilm constituents (i.e heterotroph and autotroph) contribute C and …

Pseudomonas/Brachypodium As A Model System For Studying Rhizosphere Plant Microbe Interactions Under Water Stress, Janiece Mcwilliams

Master's Theses

In contrast to well-studied mechanisms of drought tolerance in plants, the interactions between plants and their microbiome during water stress are still poorly understood. This missing knowledge is crucial for the exploitation of beneficial microbial communities to improve the sustainability of agriculture under changing climatic conditions. The research described here bridged this gap by focusing on the molecular interactions between beneficial rhizobacterium Pseudomonas synxantha 2-79 and annual grass Brachypodium distachyon Bd21. 2-79 exemplifies a group of rhizobacteria associated with dryland wheat, while B. distachyon originates from the Middle East and has emerged as a model for valuable biomass, food, forage, …

Amplifying The Prediction Of Team Performance Through Swarm Intelligence And Machine Learning, Erick Michael Harris

Master's Theses

Modern companies are increasingly relying on groups of individuals to reach organizational goals and objectives, however many organizations struggle to cultivate optimal teams that can maximize performance. Fortunately, existing research has established that group personality composition (GPC), across five dimensions of personality, is a promising indicator of team effectiveness. Additionally, recent advances in technology have enabled groups of humans to form real-time, closed-loop systems that are modeled after natural swarms, like flocks of birds and colonies of bees. These Artificial Swarm Intelligences (ASI) have been shown to amplify performance in a wide range of tasks, from forecasting financial markets to …

Vitality Of Water/Vitalidad Del Agua: Our Sacred Connection To Water And How We Can Interpret The Importance Of Water Through Artistic Inquiry, Jenifer Fogarty

Master's Theses

Water has appeared in the work and study of artists for centuries. There are countless aspects of water to be explored, from the sheer physical qualities of water to the spiritual, political, and practical associations with water. Water is what gives us life, and yet the vitality of this precious resource has been threatened over time. This exploration highlights important aspects of the study of water through art-making. My exploration began in the studio while making my own work. I sought to create a multi-sensory environment where viewers could participate and be immersed in the work itself. The site-specific installation …

Postpartum Depression In The News Media, Lauren Michelle Mcdonald

Master's Theses

The current study analyzed the relationship between gender constructs and the construction of illness in the news media. Drawing on Entman’s (1993) framing analysis, the present study assessed if postpartum depression was constructed in the news media differently for men and women. Using LexisNexis, 153 news media articles were collected from major United States news sources between 1994-2016 using keywords such as, postpartum depression and paternal depression. The content analysis indicated that the definition of postpartum depression, the cause of the illness, the moral evaluations and the prescribed treatments were related to gender constructs. Overall, these findings enhance our understanding …

Using Offline Activities To Enhance Online Cybersecurity Education, Sarah Padlipsky

Master's Theses

Since the beginning of the 21st century, the United States has experienced the impact of a technological revolution. One effect of this technological revolution is the creation of entirely new careers related to the field of technology, including cybersecurity. Continued growth in the cybersecurity industry means a greater number of jobs will be created, adding to the existing number of jobs that are challenging an under-educated and under-trained workforce. The goal of this thesis is to increase the effectiveness of cybersecurity education. This thesis studies whether an online course in cybersecurity can be enhanced by offline, in-person activities that mirror …

Cold Acclimation Response Of Non-Native Italian Wall Lizard (Podarcis Siculus) Populations From New York And California, Daniel Haro

Master's Theses

Understanding how organisms respond to climatic variability and novel conditions is becoming an increasingly important task for ecologists. For ectotherms in the northern hemisphere, the response to cold is of special interest, considering that poleward range expansion events and increasing variability of temperatures during winter are already being observed as consequences of a warming planet. Though direction of change in physiological variables in response to cold is well studied in ectotherms, the extent to which traits can change and the rate at which they can change is not.

We compared the extent and rate of change in cold tolerance (CT …

Comparative Analysis Of Electrodynamic Toroidal Radiation Shielding Configurations, Max Rosenberg

Master's Theses

Beyond the protective confines of Earth's atmosphere and magnetosphere, spacecraft are subject to constant bombardment by high-energy charged particles originating from our Sun in the form of Solar Particle Events (SPEs), and from outside the solar system in the form of Galactic Cosmic Rays (GCRs). The harm these particles do can be reduced or mitigated outright through radiation shielding. Because protons and other charged particles comprise most of these radiation particles, strong magnetic fields could be generated around spacecraft to deflect incoming charged radiation particles. This thesis investigates the performance of specific configurations of toroidal superconducting solenoids to generate magnetic …

Fluid Agitation Studies For Drug Product Containers Using Computational Fluid Dynamics, Matthew Hiroki Ichinose

Master's Theses

At Amgen, the Automated Vision Inspection (AVI) systems capture the movement of unwanted particles in Amgen's drug product containers. For quality inspection, the AVI system must detect these undesired particles using a high speed spin-stop agitation process. To better understand the fluid movements to swirl the particles away from the walls, Computational Fluid Dynamics (CFD) is used to analyze the nature of the two phase flow of air and a liquid solution.

Several 2-D and 3-D models were developed using Fluent to create simulations of Amgen's drug product containers for a 1 mL syringe, 2.25 mL syringe, and a 5 …

Molecular Dynamics Of P21 And Fluorescent Sphingomyelin In Keratinocytes Exposed To Uvb, Tyler Malcolm Fraser

Master's Theses

Non-melanoma skin cancer (NMSC) is the most common malignant tumor, representing more than a third of all malignant tumors combined and the incidence is increasing every year. Ultraviolet (UV) radiation from the sun is the most dominant factor contributing to tumor initiation and progression. The condition is most prevalent in populations with lighter skin and older age. Current pharmaceutical molecular research targets the inhibition of the Epidermal Growth Factor Receptor (EGFR), a receptor which is commonly over-expressed or dysregulated in skin malignancies. This study evaluates the content and location of the damage marker p21 within keratinocytes that were incubated in …

Indirect Food Web Interactions: Sea Otter Predation Linked To Invasion Success In A Marine Fouling Community, Maggie F. Jenkins

Master's Theses

Humans have caused grave ecological and economic damage worldwide through the introduction of invasive species. Understanding the factors that influence community susceptibility to invasion are important for controlling further spread of invasive species. Predators have been found to provide biotic resistance to invasion in both terrestrial and marine systems. However, predators can also have the opposite effect, and facilitate invasion. Therefore, recovery or expansion of native predators could facilitate the spread of invasive species. Needles et al. (2015) demonstrated that the threatened southern sea otter (Enhydra lutris nereis) facilitated the invasion of an exotic bryozoan, Watersipora subatra. …