Digital Commons Network^™

Development Of A Flow Cytometry Method For The Identification And Enumeration Of Enterococcus Faecalis, Riza Magbitang

Master's Theses

A multi-color flow cytometry method coupled with fluorescent staining was developed to detect and quantify E. faecalis concentrations in coastal waters as an alternative to the traditional membrane filtration method. The developed method was used to characterize E. faecalis during batch growth and yielded consistent results across the various physiological states of the cells. Additionally, the application of propidium iodide (i.e., a viability stain), afforded the method the ability to differentiate between cells with intact and compromised cell membranes, which raises the potential of quantifying cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked …

An Examination Of The Strong Start Intervention To Support The Pro-Social Behaviors In At-Risk Head Start Students, Merrick Mcevoy

Master's Theses

Children from lower-income and diverse families are at a higher risk for social-emotional and academic difficulties. Implementation of early childhood interventions plays a crucial role in the healthy development of children’s social-emotional development. Merrell’s Strong Kids (Merrell et al., 2009), a commonly used SEL class-wide intervention, facilitates learning of the five core SEL components through weekly lessons and has been shown to have long-term improvements on children’s internalizing symptoms, social and emotional knowledge, student-teacher relationships, prosocial behaviors, and is well received by teachers. The current study examined the efficacy of Strong Kids implemented as a one-on-one intervention, rather than a …

Peripheral Vascular Function In Young Adults With Increased Risk Of Cardiometabolic Disease, Ryan S. Aultman

Master's Theses

Objective: To determine if young adults with elevated metabolic syndrome severity scores (MetS_index) suffer from peripheral vasculature dysfunction.

Methods: Mean arterial pressure (MAP), Femoral (FBF) and Brachial Blood flow (BBF), Femoral (FVC) and Brachial Vascular Conductance (BVC), and Tissue Saturation Index (TSI) were assessed in twenty-four age (19 + 2.25 years), Sex (male: n=18; female: n=6) and Race (White: n=4; Black/African American: n=4; Asian: n=16) matched individuals during post-occlusive reactive hyperemia (RHBF), passive limb movement (PLM) and Functional Sympatholysis (FS) trials. Carotid femoral pulse wave velocity (cfPWV) was also collected at baseline, and all values were compared between …

Identifying New Potential Breeding Pond Sites For The Dusky Gopher Frog In Southern Mississippi, Shelbie Kellum

Master's Theses

The main objective of this thesis was to identify potential new pond sites across southern Mississippi for the critically endangered dusky gopher frog (DGF). Ephemeral ponds are the preferred breeding habitat for the species, but they are quite rare in the region due to the diminishment of the longleaf pine ecosystem. This research addresses gaps in the current literature by providing a broad overview of dusky gopher frog breeding habitat identification. LiDAR derived datasets as well as tools within ArcGIS Pro were utilized in order to perform spatial analysis on sites of interest in south Mississippi. By using this methodology …

On An Unshakeable Foundation: An Archaeological Investigation Of The Postemancipation Black Community Of Bass Street, The Church They Built, And The Lasting Identity They Formed In Nashville, Tennessee, Clélie Elizabeth Cottle Peacock

Master's Theses

The Bass Street Community lived along the northern base of St. Cloud Hill in Nashville, just below the Civil War-era Union fortification, Fort Negley. The fort was built and defended by conscripted free, enslaved, and self-emancipated Black/African Americans and soldiers from the U.S. Colored Troops; some of whom stayed, built residences, founded a church, and established a shared community identity. The objects they left behind reflect a time of transition in the postemancipation urban South.

The former Bass Street Community enclave is a subset of the Fort Negley archaeological site (40DV189). My thesis examines artifacts from the Bass Street Baptist …

Effects Of Increasing Cycling Cadence On Post-Cycling Gait Velocity: An Experimental Study, Nitu Lama

Master's Theses

Previous research has shown that increasing cycling cadence, rather than the workrate, can result in improved post-cycling gait velocity. However, the specific threshold of cycling cadence required to bring about clinically meaningful changes in gait velocity remains unknown. To address this knowledge gap, our study aimed to determine the minimum incremental increase in cycling cadence that would lead to a significant improvement in post-cycling gait velocity. A total of 42 young adults participated in our study and were randomly assigned to one of three groups: TEN, TWENTY, and THIRTY. Each group was assigned to cycle at a cadence at the …

The Relationship Between Overparenting, Psychological Needs Satisfaction, And Alcohol Use On Risky Sexual Behavior, Melanie Prater

Master's Theses

Studies have shown college students to be vulnerable to negative outcomes, including engaging in risky alcohol use (Lewis et al., 2014; Kilwein & Looby, 2018; Cui et al., 2019) and risky sexual behavior (Dir et al, 2014; Trub & Stark, 2017; Kilwien & Looby, 2018). Understanding predictors and mediating variables associated with risky sexual behavior is one important means of improving prevention strategies. Parenting may be one variable associated with risky sex. Overparenting, a parenting style characterized by parental overinvolvement, has received recent attention due to its association with poor outcomes for adolescents and emerging adults, including an increased risk …

Investigating The Response Of The Eastern Oyster (Crassostrea Virginica) To Low Dissolved Oxygen Conditions, Abiola Obafemi

Master's Theses

The Eastern oyster (Crassostrea virginica) requires dissolved oxygen (DO) of about 4 mg/L to support proper physiological function. Hypoxia and/or microxia occur when DO decreases to ≤ 2 mg/L. Salinity and temperature changes resulting from large-scale freshwater influx have been identified as key factors influencing the oyster populations in the Mississippi Sound. It is important to investigate the impacts of DO conditions on oyster mortality, biomass, size, and condition during these freshening events. Under controlled laboratory experiments, this thesis investigated 1) the response of oysters to microxic, hypoxic, and normoxic conditions 2) the response of oysters to combined hypoxia and …

Projected Range Shift Of The Atlantic Surfclam, Spisula Solidissima, And The Ocean Quahog, Arctica Islandica, Under Climate-Induced Benthic Warming: Forecasting Fishery Influence In The Mid-Atlantic Bight Throughout The 21st Century, Molly Mae Spencer

Master's Theses

The Atlantic surfclam, Spisula solidissima, and ocean quahog, Arctica islandica, are biomass dominant bivalve species on the eastern North American continental shelf, both supporting lucrative commercial fisheries in the Mid-Atlantic with a combined value of $53.6 million in ex-vessel revenue per year. Rapid climate change is expected to modify the geographic range of these bivalve populations across the Mid-Atlantic Bight (MAB), suggesting transformative consequences for the surfclam fishery. This study forecasted future distributions of the two species from years 2016 – 2095, based on projected bottom water temperatures and a temperature-dependent population dynamics model, to project subsequent fishery-based indicators …

Antimicrobials Produced By Ants: Chemical Properties And Microbe Specificity, Katy Chon

Master's Theses

Antibiotic resistance is a growing public health concern worldwide, and for over a decade, we have been witnessing the growth of difficult to treat infectious diseases caused by bacteria that are becoming resistant to antibiotics. This resistance can be mostly related to the misusing and improper use of antibiotics in both humans and animals. To restore this problem, humans have turned to new sources for the production of antibiotics. In this study, we focused on using ants. Social insects, including ants and bees, have faced strong disease pressures during their evolution and have developed a range of methods to fight …

Adaptable Quantum Education Platform Using Learning Objects, Krishna Puja Anumula

Master's Theses

In recent years, the need to make classroom learning more interactive and engaging has become increasingly important. The lack of workforce in interdisciplinary fields such as quantum networking and quantum internet requires a new approach that addresses every learner’s individual needs. To address this challenge, this thesis introduces an adaptive learning platform rooted in the theory of learning objects and Kolb’s experiential learning model. The platform aids educators and learners in designing and utilizing various learning objects for quantum networking and quantum internet.

The platform enables educators and learners to build their own lessons and lesson plans using learning objects …

Automated Concrete Bridge Deck Inspection Using Unmanned Aerial Systems Collected Data: A Machine Learning Approach, Rojal Pokhrel

Master's Theses

Many national infrastructures, particularly bridges, are becoming older and older day by day. Their safe operation and maintenance require routine monitoring and inspection. There are lots of bridge inspection specifications and manuals to guide inspectors to give the proper recommendations about the bridge structure and safety. These manuals and specifications require bridge inspectors to manually inspect the bridge and recommend maintenance and repair tasks referring to the specific criteria available in the manuals. In time, the application of technology makes many Unmanned Aerial Systems (UAS) advancements during the phase of data collection. For the image processing and analysis of collected …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Exploring The Relationships Between Food Insecurity, Wic Participation, Early Life Feeding Practices, Diversity Of Dietary Exposures, And Diet Quality In The First Two Years Of Life, Vivian M. Drewelow

Master's Theses

Diet quality during early childhood is a strong predictor of mental and physical outcomes, as well as future diet quality. Although many factors determine diet quality during childhood, food insecurity is a significant predictor of lower diet quality. On the other hand, participation in nutrition assistant programs, such as the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) is a significant predictor of higher diet quality. In addition, specific feeding practices, including timing of complementary food and beverage (CFB) introduction, types of CFB introduced early, and diversity of dietary exposures (DD), in the first year may predict future …

Loverboy Rules: A Transtheoretical Exploration Of Andy Bernard's Failed Masculinity, Jessie Marta Olstad

Master's Theses

NBC's U.S adaptation of The Office is one of the most well-known sitcoms to come out of modern television, surpassing its original British counterpart’s two seasons with its own nine. It is a representation, as well, of the time it aired (2005-2013), reflecting on the modern American workplace and the everyday person. Within it, The Office also reflected how society was constructed at the time with its humor surrounding gender, homosexuality, and race – as a tolerant but not welcoming atmosphere to people who were not white, heterosexual, men. More specifically, The Office presents multiple adaptations of masculinity to teach …

Neurogenomics Of Competition And Parental Care In A Socially Polyandrous Shorebird, Tessa Mathur Patton

Master's Theses

Despite sharing largely similar genomes, females and males of the same species exhibit morphological, physiological, and behavioral differences. Sex-role reversal stems from a unique case of sexual selection in which females compete for mates and males conduct the majority of parental duties. We examined the extent to which aggression and parenting may be mediated by different neurogenomic mechanisms by comparing females versus males in the northern jacana (Jacana spinosa), a sex-role reversed shorebird. We hypothesized that sex differences in neural transcriptomic profiles explain sexual dimorphism in behavior. We conducted RNA-Seq in two brain regions within the social behavior network: the …

Looking For Love: Motivations Of Dating App Usage In Millennials, Mel Ekins

Master's Theses

Dating applications (apps) have become a popular method for young adults to make romantic connections. The apps themselves are easy to use and sometimes are created to be “game-like” to entice people to want to utilize them. People can “match” with other users and strike up conversations. Extensive research has been conducted on motivations for dating and using the dating app Tinder. This study looks at users’ reasons for using Bumble, where little research has been done. This study focuses on millennials aged 26-42, whereas most other studies have focused on college students under 23. This study uses a mixed …

Digimindready: Enhancing Military Readiness Through Edge Ai-Driven Wellness, Education, And Digital Discipline Via Privacy-First Mhealth Innovation, Md Mehedi Hasan

Master's Theses

Military personnel often find themselves in intense situations that require high focus. Successfully engaging in these dangerous missions means they must efficiently control cognitive load, manage overwhelming stress, and stay focused through distractions to perform at their best. Military training significantly focuses on human performance, which benefits military readiness. The 21st century has introduced unanticipated challenges to all, such as the adverse effects of excessive screen time, external distractions, and over-reliance on technology without being aware of digital discipline. Militaries are no exception. These challenges have become an emerging threat to military personnel's cognitive, emotional, and physical well-being. On top …

Evaluating The Effectiveness Of Substance Abuse Treatment Within The Illinois Prison System And Its Effect On Recidivism, Maria Dimeglio

Master's Theses

The present study aims to evaluate the efficacy of two specific substance use disorder (SUD) treatment programs within the Illinois State prison system between July 1, 2010, and June 30, 2015. This study utilizes a quasi-experimental research design to evaluate the corrections-based Therapeutic Community (TC) programs at the Sheridan and Southwestern Illinois Correctional Centers. These programs are evaluated based on the effects they have on reoffending, specifically highlighting their effect on young individuals. The final sample used in the present study was taken from a population of 72,906 men released from the Illinois state prison system between July 1, 2010, …

Ct-To-Mri Synthesis For High-Dose-Rate Brachytherapy Treatment Planning, Rachel Nicole Gordon

Master's Theses

High-dose-rate (HDR) brachytherapy is a radiation treatment modality that places radioactive sources directly in cancerous regions. Radiation treatment planning for HDR prostate brachytherapy utilizes both CT and MRI to visualize the path of the radioactive source and the prostate gland, respectively. In this work, we propose GAN-CM, a method for conditional CT-to-MRI translation that is based on Generative Adversarial Networks (GANs). The proposed method uses the typical generator-discriminator design of GANs with a modified generator that incorporates semantic masks obtained from the domain image. The use of semantic masks allows GAN-CM to better capture the anatomical details and tissue characteristics …

Assessment Of Georgia’S Royal Catchfly (Silene Regia) Along A Forest-Edge Gradient In A Remnant Prairie Community, Megan Brooks

Master's Theses

Royal catchfly (Silene regia) is a perennial prairie forb known to grow in rocky, alkaline soils within tallgrass prairies of the eastern United States. S. regia is recorded from twelve states, including Georgia, and is now confined to fragmented habitats due to the loss of 95% of its natural environment. The species is now listed as critically imperiled in the state of Georgia. Attempts at reintroduction via outplanting have only shown limited success, necessitating more research on the environmental conditions in which this plant survives in its southern-most distribution. This study investigated the local ecological factors influencing the …

Neuroinflammation And Gut Barrier Function Post Traumatic Brain Injury, Anne Sullivan

Master's Theses

Traumatic Brain Injury (TBI) is a major cause of morbidity and mortality that afflicts millions of people in the United States annually. TBI mice had significantly increased expression of the pro-inflammatory cytokines and decreased expression of tight junction proteins CLDN5, CLDN12, and OCLD in brain lesion tissue. TBI mice exhibited a significant accumulation of FITC in the middle and distal part of the small intestine compared to sham mice. TBI mice exhibited an increase in pathogenic bacteria Enterobacteriaceae and a decrease in beneficial bacteria lactobacillus. TBI mice exhibited a trend toward an increase in permeability compared to no treatment. These …

Fleeing Towards Healing: How Cam Can Heal Refugee Communities, Sarah B. Shaalan

Master's Theses

Alternative methods of healing can be found across the globe and are used by all kinds of people including refugees. This thesis will explore the potential benefits of Complementary and Alternative Medicine otherwise known as “CAM” as a supplementary strategy for helping refugees to deal with stress, Post Traumatic Stress Disorder (PTSD) and other harmful effects sustained on their journey and lives as displaced peoples. I will do so by first situating my project in the literature on Eurocentrism in healing practices and the emergence of CAM as an alternative. Then, using extensive primary and secondary sources, supplemented by an …

The Role Of Micrornas In Lung Inflammation After Alcohol And Burn Injury, Connor O'Neal Guzior

Master's Theses

Acute lung injury (ALI) and the subsequent development of acute respiratory distress syndrome (ARDS) are among the leading causes of post-burn morbidity and mortality. MiRNAs are small non-coding RNA molecules that are known to regulate gene expression and help regulate lung homeostasis. In this study, we profiled four anti-inflammatory miRNAs (miR-146a, miR-150, miR-194, and miR-671) in lung tissue following an alcohol and burn injury. We found that miR-146a and miR-671 were significantly downregulated in lungs one day after combined injury, leading to increased lung inflammation and pathology. Biological sex did not have an effect on this pathophysiological process. We also …

Investigating Car T Cell Persistence, Functional Phenotype, And The Associations With Clinical Response In Cd19 Car T Cell Treated Patients, Oliwia Bochnacka

Master's Theses

CAR T cell therapy is the adoptive transfer of autologous T cells genetically engineered to express an artificial T cell receptor. In the clinic, CAR T cells have been most effective in treating hematological malignancies and there have been many efforts to continue improving both the safety and efficacy of CAR T cells. Using patient blood samples from the Loyola CD19 CAR T clinical trial, we studied CD19 CAR T persistence and the cell surface phenotype to identify how CD19 CAR T cells change over time in the blood of treated patients and determine whether there were trends associated with …

Development Of An Advanced Step Counting Algorithm With Integrated Activity Detection For Free Living Environments, Paige M. Dolan

Master's Theses

Physical activity plays a crucial role in maintaining overall health and reducing the risk of various chronic diseases. Step counting has emerged as a popular method for assessing physical activity levels, given its simplicity and ease of use. However, accurately measuring step counts in free-living environments presents significant challenges, with most activity trackers exhibiting a percent error above 20%. This study aims to address these challenges by creating a machine learning algorithm that leverages activity labels to improve step count accuracy in real-world conditions. Two approaches to balancing data were used: one employed a simpler oversampling technique, while the other …

A Screening Life Cycle Analysis Of One-Way And Reusable Crate Designs – Estimating Environmental Impacts Via Lca Software, Nicolas R. Corona

Master's Theses

A comparison analysis conducted via COMPASS life cycle analysis software has indicated that a one-way crate design, rather than a reusable crate design, is in fact the more environmentally friendly packaging system. These results can be interpreted differently, however, as the manufacturer of said crate designs must confirm what impact indicators they would like to reference as environmental goalposts. The conducted analysis provides insight into what the environmental impacts of each packaging system look like as packaging at all three system levels has been identified as a means of reducing environmental impacts globally. As such, the manufacturer of said crate …

Thermal Preconditioning Effects On Perforation Propensity Of Transvenous Implantable Cardiac Leads Used For Pacing And Defibrillation, Diane Muff

Master's Theses

Thermal preconditioning to simulate shipping, handling and storage conditions did not affect distal tip stiffness values for permanently implantable cardiac leads used for pacing and defibrillation. Leads that were subjected to extreme temperatures and temperature cycling did not show changes in buckle force values compared with control specimens which were maintained at ambient room conditions. Absolute differences between all measurements were small, generally under 0.05 N and were attributable to measurement variability.

Buckle force values are used to assess the propensity of leads to perforate the heart, a rare but potentially serious complication of implantable pacing and defibrillation systems. Since …

Providing Cadence Feedback In Real-Time To Guide Cardiovascular Workouts, Levi O. Rash

Master's Theses

Cardiovascular workouts offer numerous health benefits, yet beginners often find it challenging to initiate them. Existing wearable technologies, although providing valuable feedback such as heart rate zones, often disrupt the workout flow and distract users due to the need for interaction with the wearable display. In response, we propose an alternative feedback mechanism: cadence, measured in steps per minute. This feedback mechanism uses multiplicative control to produce the correct cadence for the user’s target heart rate (HR). To model the HR and cadence relationship, a first-order system was used. The prototype implementation of this system was completed in Arduino, using …