Digital Commons Network^™

Examining Opportunity In The Dental Community To Identify Prediabetes, Kirsten S. Carew

Master's Theses

Abstract

BACKGROUND: In response to the growing epidemic of prediabetes and diabetes (type 2), the State of Connecticut passed Public Act 19-72 “An Act Concerning Dental Practitioners” effective July 1, 2019 allowing licensed dentists to administer an in-office blood screening test to consenting patients identified as having an increased risk for diabetes. The intent of this law is to expand the venues to screen, identify and inform those at-risk for disease.

METHODS: An anonymous online cross-sectional pilot study was conducted utilizing membership of major statewide dental societies to recruit licensed practicing dentists in the state of Connecticut. Participants completed a …

Nf-Κb Subunits P50 And Rela Contribute To Oncogenesis In A Mouse Model Of St-Epn, Ericka J. Randazzo

Master's Theses

The NF- κB pathway has been demonstrated to be upregulated in supratentorial ependymoma (ST-EPN). Whether the different NF- κB transcription factors contribute to ST-EPN tumorigenesis however, has not been investigated. We assessed the degree to which NF- κB subunits p50 and RelA interact with the ST-EPN fusion mutation C11orf95-RELA in regulating gene expression via colocalization analysis in Hek293T cell nuclei. We analyzed the functional outcomes of overexpression of p50 or RelA, or CRISPR/Cas9-mediated knockdown of p50, in combination with the C11orf95-RELA fusion in vivo by examination of tumor area in P14 – 21 CD1 mice. We further analyzed the …

The Harms Of Willful Ignorance: Maternal Healthcare Providers’ Insight On Racial Disparities, Cara Marisa Cancelmo

Master's Theses

Black women in the United States die in pregnancy and childbirth at alarmingly high rates. According to The Center for Disease Control, black women are two to three times more likely to die from pregnancy-related causes than white women. The black-white racial disparity is even greater in New York City, and The NYC Department of Health has found that black mothers in NYC still face higher rates of harm even when accounting for risk factors like educational attainment, obesity, and neighborhood poverty level. Drawing on 18 interviews with NYC-based Obstetricians, Labor and Delivery Nurses, and Midwives, this study examines how …

New Childhood Chronic Illness: Pans/Pandas And The Impact On Family Functioning, Jamie L. Dolce

Master's Theses

The current study examined parents’ reports of change in the parent-child relationship and family functioning following the onset of Pediatric Acute-Onset Neuropsychiatric Syndrome (PANS)/Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal Infections (PANDAS) in their child. Research questions explored 1) how families’ day-to-day lives and functioning changed since the onset and 2) what challenges the families faced in raising a child with PANS/PANDAS. Research was conducted in two phases: narrative thematic analyses of online parent forum threads followed by semi-structured interviews with parents. Four primary themes were compiled from analyses of both data sources: Determinants of Family Functioning; Close Relationships; Routines …

Genome-Wide Rnai Screen Of Kinase Pathways Regulating Rrt-1 Constitutive Cytoprotective Gene Expression, Jacob Bowie

Master's Theses

Environmental stress disrupts cellular homeostasis leading to proteostasis disruptions which must be correct by rapid reprogramming of transcription and translation mediated by the translation machinery of which tRNA and aminoacyl tRNA synthetases are particularly important. Our aim in this study was to conduct a genome-wide RNAi kinase screen of a long-lived and stress-resistant rrt-1(gc47) (MC240) C. elegans mutant that constitutively, under naïve conditions, expresses a GFP reporter for gpdh-1, which is required for survival during hypertonic stress, but not expressed under naïve, non-stress conditions. Our approach was to administer RNAi by feeding, and we used 382 RNAi clones, including genome-wide …

Intersecciones Entre Cine Documental Y Archivos Queer: Notas A Propósito De Sexilio., Lazaro J. Gonzalez

Master's Theses

For decades, documentary filmmakers have combined academic research and narrative non-fiction, making the genre of documentary a resourceful tool to address certain agendas that exceed the boundaries of traditional research methodologies. My feature film Villa Rosa (2016) as well as the work in progress of Sexilio (2020…) combine the scholarly and cinematographic realms as a strategy to foreground queer issues and the preservation of individual and collective memory. More specifically, my thesis will focus on rhetorical strategies used by queer filmmakers and archivists when the so-called historical archive appears deficient. As a result, the filmmaking process itself must create the …

Altered Gene Expression In Circulating Immune Cells Following A 24-Hour Passive Dehydration, Aidan Fiol

Master's Theses

Dehydration has many deleterious effects ranging from impaired cognitive function to decreased aerobic performance. This study evaluated the effects of a mild, passive dehydration on the transcriptional responses of circulating immune cells. We pursued research questions about how whole-body dehydration and small changes in plasma osmolality may affect the greater concept of immune resiliency or susceptibility to infection through acute gene expression changes that may persist during chronic stress. Our approach was to precisely quantify hypertonicity experienced by circulating peripheral blood mononuclear cells and neutrophils and quantify transcriptomic changes in cell lysates using RNAseq. RNAseq revealed 373 total genes changing …

Mir-433-3p Negatively Regulates Bone Formation And Targets Critical For Osteoblast Function, John Garcia

Master's Theses

Osteoblastic differentiation is tightly controlled by multiple signaling pathways associated with key transcription factors, as well as a number of microRNAs (miRNAs). miRNAs are small noncoding RNAs that function in the post-transcriptional regulation of gene expression. While clinical strategies to combat the reduction in bone density have often centered on impeding further bone resorption, this strategy neglects to promote bone formation, a component which is particularly problematic in the aging population. miRNAs could serve to bridge that gap, as potential therapeutic candidates. Several studies have established that miRNAs can direct osteoprogenitors towards an osteoblastic lineage through modulation of Wnt, MAPK, …

State Of Community Health Needs Assessments In Connecticut, Miryam Hannah Wilson Reardon

Master's Theses

No abstract provided.

A Serial Multiplexed Approach To Immunolabeling Brain Tissue For Electron Microscopy, Peter Tiley

Master's Theses

To fully understand how biological systems give rise to complex phenomena, both structural and functional knowledge of the components in the system must be acquired. In this manuscript, I explain why new methods must be developed to achieve this level of understanding with brain tissue and introduce serial multiplexing as a potential solution. I discuss how this method can lead to highly annotated volume reconstructions of brain tissue using electron and light microscopy, along with the steps required to do so. I present what our lab has found throughout the process of adapting, refining, and combining more traditional methods together …

The Denial Of Black Victimhood: Examining Attitudes Of Sexual Assault And Victim-Blaming On A College Campus, A Continued Analysis, Odia Kane

Master's Theses

This study examines the attribution of guilt and victimhood in the context of rape on predominately white college campuses. The study is comprised of two samples; Sample 1 (n=362) and Sample 2 (n=1,144) of students from a New England university. Each participant read a vignette regarding two classmates: a female victim, Taneesha or Madeline, and a male perpetrator, Jamal or Connor, completing a group project. The findings of this study are critical because they analyze the surrounding attitudes of the treatment of victims of sexual assault and assess the social and political climate of rape culture on college campuses.

Associations Of Cardiovascular Health, Strength, And Physical Activity In Participants With A History Of Anterior Cruciate Ligament Reconstruction, Kirsten Allen

Master's Theses

No abstract provided.

Triplet-Triplet Annihilation-Based Photon Upconversion In Protein Biomaterials, Alexander M. Oddo

Master's Theses

No abstract provided.

Innovation Dynamics Of Cultural Production: Evidence In Rap Lyrics, James M. Zumel Dumlao, Junjie Lei, Emeka Nwosu, Li Yu Oon, Tsai Ling Jeffrey Wong, James Rising, Jesse Anttila-Hughes

Master's Theses

Culture is a driving force in organizing the structure of societies, and is conjoined with economic development. However, quantifying the impact of culture is difficult. Culture manifests itself in cultural production, through art, performance, music, etc. Innovation and influence in cultural production industries partially determines product quality. Using techniques from the “digitized humanities”, we agnostically identify informational distance to describe the spatiotemporal dynamics of innovation and influence in Rap music lyrics. Rap emphasizes lyricism and hometown pride more than other genres of popular music, and is interesting as a globally impactful manifestation of the racially segregated labor market in the …

Actors As Engineers: The Reconstruction Of Antifascism In Defa Films, 1949-1961. [2020], Jonathan Herr

Master's Theses

The East German film industry (led by state film company DEFA) had, since its inception in 1946, focused heavily upon the theme of antifascism. However, the meaning and definition of antifascism changed dramatically over the course of East Germany’s early history. In DEFA’s earliest days, antifascism was a confrontation of Germany’s Nazi past and argued that capitalism was a forebearer to fascism. As the East German state formed, antifascism evolved, casting America and its unchecked capitalism as the enemy to democracy. Here DEFA films still confronted Germany’s dark past, though the end goal of the films was to promote hope …

A Comparison Of Reasons For Breastfeeding Cessation In Preterm And Term Infants During The First Year Of Life, Rebecca Robert

Master's Theses

The purpose of this study was to compare factors that may affect breastfeeding duration or cessation in preterm and term infants during the first year of life. A 50- question survey was administered using SurveyMonkey™ and distributed through social media platforms. Participants were able to enter a drawing for an Amazon gift card as incentive. SPSS was used for statistical analysis. Participants included mothers over the age of 18, with a child between the ages of 12 and 24 months, born in the U.S., and who provided human milk to their child at any point during the first year of …

Optimization Of A Gfp Biosensor For The Discovery Of Novel Antibiotics, Drake Heinz

Master's Theses

In today’s world, there is an urgent need to discover novel antibiotics due to the rise of antibiotic resistance and the lack of new ones being approved. Building off of Hanson et al. and their reduction-oxidation sensitive green fluorescent protein (roGFP)(1), that we yeast codon optimized (2); I wanted to determine if I could create a real-time biosensor that has the ability to respond to ROS produced in the stress response triggered by antibiotics as a platform to identify novel antibiotics. To achieve this, I need to know if it will oligomerize in bacterial cytoplasm due to its two cysteines …

Cascaded Linear Regulator With Positive Voltage Tracking Switching Regulator, Brandon K. Nghe

Master's Theses

This thesis presents the design, simulation, and hardware implementation of a proposed method for improving efficiency of voltage regulator. Typically, voltage regulator used for noise-sensitive and low-power applications involves the use of a linear regulator due to its high power-supply rejection ratio properties. However, the efficiency of a linear regulator depends heavily on the difference between its input voltage and output voltage. A larger voltage difference across the linear regulator results in higher losses. Therefore, reducing the voltage difference is the key in increasing regulator’s efficiency. In this thesis, a pre switching regulator stage with positive voltage tracking cascaded to …

Small Universe: Exploring Microorganisms In Water From An Artistic Perspective, Jingying Zhen

Master's Theses

The scope of this thesis was based on an artistic desire to explore and display microorganisms in water and present the viewers with an opportunity to discover an invisible world. This included research on three artists who have taken interest in unusual life forms. It also included my observation of real microorganisms under a microscope in biology lab. I found the beauty within the details of this miniature world; it was a different perspective. The purpose of this study was to combine biology and art, traveling in the real and imaginary world, researching, exploring, and then showing microorganisms using multiple …

Thiol-Ene Click Chemistry For Solid State Triplet-Triplet Annihilation Upconversion And Parts-Per-Billion Pyrophosphate Sensing In Artificial Seawater, Abagail K. Williams

Master's Theses

Thiol-ene click chemistry is a robust approach to molecularly engineering polymers for many applications. Within this work, thiol-ene click chemistry is used to fabricate thiol-ene networks for TTA-UC and to synthesize a conjugated polyelectrolyte (CPE) used as a pyrophosphate (PPi) sensor in complex aqueous media. Chapter I focuses on the synthesis and upconversion performance of rubbery networks fabricated using thiol-ene click photopolymerization. The advancement of triplet-triplet annihilation based upconversion (TTA-UC) in emerging technologies necessitates the development of solid-state systems that are readily accessible and broadly applicable. We demonstrate that thiol-ene click chemistry can be used as a facile cure-on-demand synthetic …

Mosquito Communities And Insect-Specific Viruses In A Heterogenous, Urban Landscape In Puerto Rico, Nicole Scavo

Master's Theses

The goal of my thesis was to investigate the relationships between mosquito diversity, Aedes aegptyi populations, and socioeconomic factors (SEF) in an urban setting and to characterize the virome of Ae. aegypti in San Juan, Puerto Rico. Mosquitoes were collected in three periods during late 2018 and early 2019 and were identified to the species level. Socioeconomic factors were measured using foot surveys and U.S. Census data. Some Ae. aegypti females were processed for sequencing and sent to Quick Biology for RNA sequencing to identify viruses. Results showed a link between SEF and Ae. aegypti abundance as well as between …

Significant Objects As Metaphor For Home, Alison Wilcox

Master's Theses

This qualitative Arts-Based Research investigated how certain objects come to embody a personal definition of home. Objects that are treasured and are associated with home were defined in this research as significant objects. As the researcher, I selected personally significant objects to study. These objects were studied through still life painting and interviews, based on the work of Barbara Fish (2017). Data were then refined into a final reflection painting. This study indicated that significant objects are often denied their intended function, are tied to idealized memories, and help to reconnect the owner to confidence that may have been lost …

Zebrafish Eye Development: Rac And The Creepy Crawlers Of The Eye, Zvi Murry

Master's Theses

During vertebrate eye development the optic vesicles protrude from either side of the brain and form the optic cups. As an optic cup starts to surround the lens a groove on the ventral side of the eye forms, known as the choroid fissure (CF). Normally, the CF will close around the optic nerve and hyaloid vasculature. If this process does not occur properly it results in a keyhole opening in the eye known as coloboma. This results in blindness and affects nearly 1 in 4-5,000 births. Zebrafish were utilized as a model for eye development to study CF closure (CFC) …

Dracula Trio, Justino Perez

Master's Theses

Dracula Trio is a three-movement work for violin, violoncello, and piano-forte. This work follows an abbreviated plot of the popular video game and animated show by the name of Castlevania. Castlevania follows Dracula, a powerful vampire that falls in love with a human woman. After their marriage, Dracula’s wife is murdered by fellow humans after she is suspected of being a witch, which causes Dracula to seek revenge on all of humanity. Characterized by contrasting harmonic vocabulary and textures, the work seeks to bring to music the dramatic arc of the Castlevania story line using composition techniques from the last …

Maturation Spawning And Larviculture Of Atlantic Croaker (Micropogonias Undulatus) At Low Salinity, Edithrose Fairweather

Master's Theses

Atlantic Croaker is a popular baitfish among anglers targeting a variety of gamefish in the Gulf of Mexico. Aquaculture of this species could help meet the demand for Croaker, especially during winter when wild-caught Croaker are unavailable. Currently, Croaker aquaculture involves broodstock maturation, spawning, and larval culture at high salinity. This protocol prevents the direct use of coastal water for culture along the Mississippi Gulf coast where salinity is low. This study examined the feasibility of performing hatchery production of Atlantic Croaker at low salinity.

Culture of Croaker broodstock at 10, 20, and 30 psu during the maturation period was …

Online And Offline Identity Gaps: Cross-Contextual Predictors And Psychological Outcome, Ningyang Wang

Master's Theses

Using Communication Theory of Identity as a framework, this study compared an individual’s online and offline personal-enacted identity gap and examined the effect of each personal-enacted identity gap on individuals’ psychological well-being, as well as tested four cross-contextual predictors for online and offline personal-enacted identity gaps.

Survey data were collected from 214 participants on Amazon M-Turk. A sequence of hypotheses was tested. A questionnaire used for measuring individuals’ online personal-enacted identity gap was generated.

The result suggested individuals experience larger personal-enacted identity gap offline, which significantly negative predict individuals’ psychological well-being, whereas online personal-enacted identity gap positively predicts psychological well-being. …

Development Of An Attenuated Zika Virus By Editing The 5’ Untranslated Region, E. Ashley Thompson

Master's Theses

Zika Virus (ZIKV) is a mosquito-transmitted flavivirus that usually causes no symptoms to mild febrile in humans, and it has been regarded as an insignificant pathogen to public health. However, recent outbreaks of ZIKV infection have revealed that ZIKV can cause severe neurological effects in adults, such as Guillain-Barre Syndrome (GBS), and in infants whose mothers acquired the virus during pregnancy, causing Congenital Zika Syndrome (CZS). Currently, no approved vaccine is available and there is a critical need to develop an effective and safe vaccine. While most vaccine developmental strategies target the viral prM-E protein of ZIKV, we aimed to …

Characterizing Erad And Antioxidant Response In Ixodes Scapularis Ise6 Cells During Borrelia Infection, Latoyia Downs

Master's Theses

Ticks are hematophagous ectoparasites that transmit a multitude of diseases to humans. Borrelia burgdorferi (BB) and Borrelia miyamotoi (BM) are both tick-borne pathogens that cause disease in humans and are transmitted by the black-legged tick (Ixodes scapularis). A byproduct of blood digestion generates reactive oxygen species that are toxic and cause oxidative stress which promotes cellular damage and dysfunction. The endoplasmic reticulum (ER) is especially affected by oxidative stress, resulting in a buildup of improperly folded proteins in the ER lumen called ER stress. To prevent cellular damage, the tick utilizes an antioxidant system to neutralize ROS and mechanisms …

Barriers To Health: Understanding The Barriers Faced By Community Intervention Projects, Vera Landrum

Master's Theses

Health disparities affect significant portions of the population and are most often experienced by marginalized communities (Wilkin, 2013). Health disparities are also impacted by geographical location, and hunger often affects rural areas (Dutta, Anaele, & Jones, 2013; Rural Health Information Hub, 2017). This study aims to understand how nonprofit organizations focused on addressing food insecurity disseminate information to members of the local community and how the input of community members impacted the overall food insecurity campaign. This study was conducted by interviewing five nonprofit directors and conducting three focus groups with clients of the. aforementioned organizations. The author found that …

Oh, My Stars: A New Map Of The Universe In Paradise Lost, Michael R. Coats

Master's Theses

Milton’s geographical descriptions in Paradise Lost are heavily influenced by his fascination with maps. Furthermore, his design of the universe in Paradise Lost follows a cartographic style that has led to several attempts at mapping it. These attempts, however, have followed the erroneous assumption that Earth centers the universe. As a result, cosmographical maps of Paradise Lost are inaccurate. I argue that Milton’s universe is Deocentric and provide a new map with a pyramid design that places God at the center of Milton’s universe.