Digital Commons Network^™

Synthesis Of Functionalized Ionic Liquids For Coal Dissolution And Pretreatment, Michael Franklin

Master's Theses

Brown coal (lignite) is a bulk organic mixture of conjugated hydrocarbons that are complexed together via hydrogen bonds. Coal [partial] dissolution is essential to the better utilization of low-rank coal for power sources because the direct combustion of brown coal is not energy efficient. To break hydrogen bonds of low-rank coal and make it partially soluble, this project evaluates a series of ionic liquids (ILs) with specific properties as non-volatile alternatives to conventional organic solvents. A series of nitrogen- and phosphorus-based cations have been synthesized via a nucleophilic substitution reaction, the resultant bromide-based IL being converted to an acetate-based IL …

Warm Or Chilly? An Assessment Of The Relationships Between Campus Climate And Gender Identity Among Transgender And Nonbinary College Students, Emma Allen-Morgan

Master's Theses

This research is a mixed-methods analysis of the relationship between campus climate and gender identity for transgender and nonbinary college students. Snowball and convenience sampling were used to obtain a sample of trans and nonbinary college students. Thirty surveys and six interviews were completed. I applied Bronfenbrenner’s ecological theory and Risman’s gender structure theory to analyze how macrosystems, microsystems, and the chronosystem (time) affected trans students’ gender identity, whether positively or negatively. Participants were ambivalent about the effects of college on their gender identity; on one hand, college gives trans students freedom from their previous environments. However, participants described a …

Projecting Ecuadorian Cultures In Andean Dream: A Compositional Exploration Of Psycho-Ethnomusicology For Electronics And Chamber Ensemble, Daniela Paez

Master's Theses

The musical culture of Ecuador, despite being quite unknown to the world and to a great part of the Ecuadorian people, holds an unimaginable richness and diversity rooted in thousands of years of musical traditions. Mestizaje, the process of racial mixture that followed the European conquest, has encouraged homogeneity and uniformity. I am a part of this mestizo culture, that, in reality, does not hold the same worldviews as the diverse Indigenous Ecuadorian communities. The Kichwa community of Cotacachi, presented through musical materials in this thesis, experiences an undeniable connection between the rituals relevant to agricultural life, dance and music …

Cannabigerol Attenuate Methionine/Choline Deficient Diet- Induced Non-Alcoholic Steatohepatitis Symptoms In C57bl/6 Male Mice Via Modulating The Expression Of Cannabinoid Receptors, Nouf Aljobaily

Master's Theses

Non-Alcoholic Steatohepatitis (NASH) is the advanced and more aggressive form of Non-Alcoholic Fatty Liver Disease (NAFLD). NASH is associated with severe hepatic fibrosis and inflammation. Methionine/choline deficient (MCD) high fat diet is known to induce NASH in a short period of time without showing signs of metabolic syndrome. Cannabigerol (CBG) is a plant-derived, non-psychotropic cannabinoid that has a potential anti-inflammatory effect. Other hemp extracts reduce the progression of NAFLD to NASH, whereas the impact of CBG on NASH is still unknown. Therefore, these studies aim to 1. Evaluate the therapeutic potential of CBG on reducing hepatic steatosis and fibrosis. 2. …

Honor Crimes: A Question Of Honor, Culture, And Humanity, Nadia N. Almusleh

Master's Theses

Honor crimes, femicide, domestic abuse and violence are widely prevalent in patriarchal societies. Middle Eastern cultures deeply value protecting the chastity and honor of women. The traditional images of women and the notions of honor and shame are consistently used as justifications for violence and killings. This is not attributable to a single culture or religion. It is rather a manifestation of societal norms around gender-based violence. Feminist activism against honor crimes in the Middle East within the last decade has increasingly received social media attention. However, the impact of this social media activism on government intervention has yet to …

The Effects Of Sexual Surrogacy On Satisfaction, Happiness, And Well-Being, Ryan Liu-Pham

Master's Theses

The study tested the effects of sexual surrogacy, which I define as the desire to fulfill sexual needs with a surrogate target (e.g., celebrity crushes), on sexual satisfaction, relationship, happiness, and well-being. To examine this topic, I conducted a cross-sectional experimental study. After being asked about sexual desire toward either their current partner or a celebrity crush with a sexual desire behavior inventory, participants were asked to answer questions about their sexual satisfaction, relationship satisfaction, happiness, and well-being. I predicted that desire toward both surrogates and interpersonal targets will predict higher levels of sexual satisfaction, happiness, and well-being but that …

An Interdisciplinary Approach: Schizophrenia Derails Heteronormative Expectations In Psychological Narratives 2021, Bobbie Jo Weaver

Master's Theses

Required introductory psychology courses teach students a general and oversimplified version of the immense number of subfields within Psychology studies, much like introductory literature classes compress different genera throughout history into a miniscule number of “representative” texts. Nevertheless, these footholds generate an entryway into a whole new world of (specialized) exploration. Reading a text such as The Quiet Room: A Journey out of the Torment of Madness by Lori Schiller and Amanda Bennett provides a window for many students to crawl into one of Psychology’s darkest shadows, the field of abnormal psychology. Schiller’s non-fictional memoir, The Quiet Room, tells readers …

Found Media: Interactivity And Community In Online Horror Media 2021, Jax Mello

Master's Theses

Being isolated is a common fear. The fear can take many forms, from the fear of being the last one alive in a horrific situation to being completely deserted by everyone you love. This is a fear that has been showcased many different times in movies, novels, and every other piece of media imaginable. Although not always tied to the horror genre, the fear of being isolated is tightly intertwined with many horror stories. Therefore, it is interesting when a horror production goes out of their way to encourage interactivity within its audience. This goes beyond an artist’s desire for …

The Role Of Med12, Notch1, And Notch3 In Adipose Stem Cell Self-Renewal And The Integrated Use Of Stem Cells In Public Educational Materials, Rebecca Hodnett

Master's Theses

Human adipose-derived mesenchymal stem cells offer significant therapeutic potential as an ethically sound, easily accessed source of adult stem cells. To harness their medically relevant properties, it is necessary to understand the mechanisms that control their fate. Cell state and differentiation of stem cells is determined by interactions of signaling pathways, chromatin modifiers, and transcription factors working to regulate cell-type specific gene expression profiles. Specifically, both the MED12 subunit of the Mediator complex and the Notch signaling pathway are known to individually influence hASC self-renewal. We investigated the relationship between Notch signaling and transcriptional cofactor, MED12, to elucidate a potential …

Relationship Between Calcium Consumption From Dairy And Non-Dairy Sources On Bone Mineral Density Of College Students, Andrew M. Pescovitz

Master's Theses

Dairy product consumption has been on the decline for decades in the US and worldwide, and the increase in plant-based substitutes has grown substantially. Osteoporosis and the risk of bone fractures are serious public health issues. To date, little scientific-based studies have compared the relationship between calcium intake from dairy sources vs non-dairy sources and bone mineral density. The purpose of this study is to compare the relationships between calcium consumption from dairy vs. non-dairy sources and bone mineral density among college students. There was a total of 66 participants in the study including 15 (23%) males and 51 (77%) …

Studying The Efficacy Of An Injectable 3-Dimensional Fibrin Extracellular Matrix To Characterize The Effects Of Antitumor Agents On Sw620 Cells In A Microfluidic Device, Thèo Anastos

Master's Theses

Colorectal cancer is the third most common cancer in the United States and there is currently a lot of research going into new antitumor agents to kill the cancer. One method for replicating the tumor response to a drug in vivo is by creating an in vitro drug testing model to replicate the in vivo condition. This research project was conducted to determine the efficacy of testing tumor cultures in a microfluidic device as a way to provide accurate drug responses in vitro instead of using in vivo subjects in clinical trials. A total of four experiments were conducted with …

Factors Leading To Structure Loss On The Thomas Fire, Rodolfo Uribe

Master's Theses

The recent surge in fire activity and the extent of displaced communities as a result of wildfire has increased awareness of wildfire issues nationwide (Syphard et al., 2017). Climate change, population growth, and continued development in the wildland urban interface (WUI) has contributed to a growing body of research into the underlying causes of this continued destruction (Kramer et al., 2019). There is no doubt that statewide policies, such as defensible space or building regulations, are associated with home survival (Keeley & Syphard, 2019). However, the relative effectiveness of wildfire mitigation depends on a myriad of factors specific to individual …

The Effects Of Wort Oxygenation Scenarios On Fermentation Performance, Volatile Flavor Compound Development, And Flavor Stability In High Gravity Brewing, Ben Jabson

Master's Theses

High gravity (HG) brewing has become the most used strategy for maximizing fermenter productivity in commercial brewing. While HG brewing has many benefits, the additional stress placed on the yeast due to the higher concentration of fermentable sugars in the wort can negatively impact fermentation performance and flavor compound formation. A proper dissolved oxygen (DO) level is vital to guarantee adequate yeast performance during HG fermentations. Dissolved oxygen is vital to yeast viability throughout the fermentation process, as yeast requires oxygen to synthesize vital cell membrane components needed for continued anaerobic growth and cell division. Previous research has demonstrated the …

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

The Response Of Marine Synechococcus To A Landscape Of Environmental Stressors: A Proteomic Exploration, Dana E. Michels

Master's Theses

In the field of marine microbial ecology, many questions remain unanswered with regards to the physiological trade-offs made by phytoplankton to maximize growth (e.g., nutrient acquisition) and minimize loss (e.g., predation defenses). These tradeoffs, which occur at the cellular level, have wide reaching impacts on food web dynamics and global biogeochemical cycles. In the first chapter, we explored the use of a non-canonical amino acid (NCAA) technique, bioorthogonal non-canonical amino-acid tagging (BONCAT), in phytoplankton model systems. This technique has potential to work well in natural systems by enabling isolation of only newly synthesized proteins during an incubation period with the …

Machine Learning Approaches To Historic Music Restoration, Quinn Coleman

Master's Theses

In 1889, a representative of Thomas Edison recorded Johannes Brahms playing a piano arrangement of his piece titled “Hungarian Dance No. 1”. This recording acts as a window into how musical masters played in the 19th century. Yet, due to years of damage on the original recording medium of a wax cylinder, it was un-listenable by the time it was digitized into WAV format. This thesis presents machine learning approaches to an audio restoration system for historic music, which aims to convert this poor-quality Brahms piano recording into a higher quality one. Digital signal processing is paired with two machine …

Development Of A Robust Methodology To Obtain And Assess Myogenic Precursor Cells For Their Use In Regenerative Therapies, Ricardo Lasa

Master's Theses

Peripheral arterial occlusive disease (PAOD) is characterized by buildup of atherosclerotic plaque in peripheral arteries that leads to an occlusion that can interrupt the supply of blood to the peripheral tissue, causing downstream tissue ischemia/hypoxia. PAOD is estimated to affect over 200 million patients worldwide. Current surgical revascularization treatments can be effective in about half of the patient population, leading to a significant number of patients with no treatment options beyond pharmacological intervention and lifestyle modification. The decrease in blood flow downstream of the occlusion leads to increased blood pressure gradient in the microvasculature, specifically in vessels that connect arterial …

Experimental Investigation Of The Effects Of Acoustic Waves On Natural Convection Heat Transfer From A Horizontal Cylinder In Air, Katherina V. Prodanov

Master's Theses

Heat transfer is a critical part of engineering design, from the cooling of rocket engines to the thermal management of the increasingly dense packaging of electronic circuits. Even for the most fundamental modes of heat transfer, a topic of research is devoted to finding novel ways to improve it. In recent decades, investigators experimented with the idea of exposing systems to acoustic waves with the hope of enhancing thermal transfer at the surface of a body. Ultrasound has been applied with some success to systems undergoing nucleate boiling and in single-phase forced and free convection heat transfer in water. However, …

Options For Low-Cost Manufacturing And Safer Cell Therapies, Melis Keceli

Master's Theses

The proposed work aims to overcome the economical and feasibility-related limitations of the chimeric antigen receptor therapies by developing an artificial cell signaling pathway whose design transforms K562 cells into in vivo living vectors to synthesize therapeutic proteins upon engaging diseased cells in the treatment of ovarian cancer. There are various advantages of using K562 cells throughout this process. First, Food and Drug Administration approves the reinfusion of K562 cells into patients’ bodies. Second, K562 cells are more affordable than T lymphocytes, and finally, these cells can be easily manipulated with any desired genetic material and can keep the expression …

Investigating The Function Of Carboxypeptidase O Gene Paralogs From Xenopus Tropicalis, Ritchie Saint Jean

Master's Theses

Carboxypeptidase O (CPO) is a peptidase that is a member of the A/B subfamily of the metallocarboxypeptidases. This peptidase has been found in the brush border of the intestine and has specificity toward C-terminal acidic residues. CPO has been proposed to be involved in intestinal digestion and the formation of lipid droplets. Four copies of the CPO gene are present in the Xenopus tropicalis (tropical clawed frog) genome and are noted as CPO.1, CPO.2, CPO.3, and CPO.4. Not much is known about these genes regarding their properties and functions. In my research, I sought to investigate the properties and functions …

Andrews University Communication Under The Covid-19 Pandemic: Feedback Analysis Of International Students, Brice B. Eboule

Master's Theses

This research is an informative analysis of international students’ feedback on the Andrews University (AU) crisis communication during the COVID-19 pandemic in 2020. Participants were 21 years or older, on student visas, living on-campus or in student communities and had attended AU for at least 2 years. The theory informing this study is situational crisis communication theory (SCCT). The SCCT argues that the severity of the crisis defines which communicational crisis strategy will be more effective in minimizing attributions and adverse press while both maintaining and enhancing the organization's credibility. The findings of this study indicated that 84 percent of …

The Effect Of Virtual Learning On Parents During Covid-19, Sheila Reeves

Master's Theses

Parents were affected in numerous ways by the sudden shut down of schools and switch to virtual learning in March 2020 due to the COVID-19 pandemic. Some of these ways included use of technology, income, race, time, mental health, family life and parent insecurities. This study investigates parents' perspectives of some ways they were affected in regards to the virtual learning of their child(ren). All parents with children enrolled at Madison Campus Elementary School were invited to participate in the study, as they had at least one year's experience with their child(ren) in virtual learning.

Data was collected using a …

Characterizing A Novel Cocksackievirus B3 Protease Mutant And Its Response To Polyamine Depletion, Bridget Hulsebosch

Master's Theses

Enteroviruses, including Coxsackievirus B3 (CVB3), are pervasive pathogens that cause significant disease, including cardiomyopathies. Unfortunately, no treatments or vaccines are available for infected individuals. We identified the host polyamine pathway as a potential drug target, as inhibiting polyamine biosynthesis significantly reduces enterovirus replication in vitro and in vivo. Here, we show that CVB3 is sensitive to polyamine depletion through the polyamine analog diethylnorspermidine (DENSpm) which enhances polyamine catabolism through induction of polyamine acetylation. We demonstrate that CVB3 acquires resistance to DENSpm via mutation of the 2A protease, which enhances proteolytic activity in the presence of DENSpm. Resistance to DENSpm occurred …

Heteronormativity In Healthcare: Neoliberalism, Bio-Politics, And Necropolitics, Grace Avila

Master's Theses

This thesis will discuss heteronormativity in healthcare. It will take a social science and theoretical approach to acknowledge LGBTQ+ health disparities, apply theoretical approaches to why these disparities exist, and offer solutions to combat health disparities experienced by sexual and gender minorities. Neoliberalism, bio-politics, and necropolitics will be the theoretical approaches utilized to understand the basis of disparities experienced by the LGBTQ+ community. Neoliberalism will include themes of capitalism and public health policies which influence discrimination in healthcare. Bio-politics will outline how the intersection of the biological body and politics can exacerbate health disparities through the power of controlling bodies. …

Anti-Unionism And The Chicago Teachers Union, Paul Stromberg

Master's Theses

The Chicago Teachers Union (CTU) is one of the most influential political organizations in Illinois. Like other political organizations, the CTU influences policy through activism, advocacy, and endorsements. Unlike most political organizations, however, the CTU's 20,000 members are responsible for carrying out policy decisions through their roles as teachers, paraprofessionals, and clinicians in Chicago Public Schools (CPS). In response to this influence, anti-unionists have become increasingly adept at criticizing the CTU, using anti-union rhetoric to malign unionized teachers. Simultaneously, anti-unionists have utilized anti-union litigation to disable teacher unions, stripping them of guaranteed protections. Assisted by conflict theory, this study examines …

Plasmodium Tlap-2 Is A Microtubule-Associated Protein With Microtubule Stabilization Properties, Grifin Berge

Master's Theses

Microtubules facilitate major cellular and morphological changes in eukaryotic cells, including the protozoan parasite Plasmodium. Microtubule-associated proteins (MAPs), only very few of which have been characterized in the malaria parasite, regulate the highly dynamic microtubules. Thioredoxin-like associated protein 2 (TLAP2) is a MAP unique to the phylum apicomplexa, which in addition to Plasmodium includes the important human pathogen Toxoplasma. Here we report on the cloning and microtubule-binding activity of TLAP2 of the rodent malaria parasite Plasmodium berghei (PbTLAP2). PbTLAP2 is coded by a single exon gene and consists of 598 amino acids. The protein contains a putative C-terminal microtubule-binding domain. …

Chga-Depleted Urothelial Amp And Immune Response During Urinary Tract Infection, Theadora Jane Ceccarelli

Master's Theses

Women are 8 times more likely than men to experience a urinary tract infection (UTI) with up to 60% of women reporting a UTI in their lifetime. This significant healthcare burden is caused by the infection of uropathogenic Escherichia coli in the urinary tract. At this time, treatment is limited to antibiotic therapy, which is challenged by antibiotic-resistance. These factors make UTIs an NIH priority and are motivation for our research.CgA is a protein of the granin family co-released with catecholamines from neuroendocrine cells throughout the body. Pro-hormone convertases post-translationally cleave CgA into peptides, like catestatin, which can have antimicrobial …

The Identification Of Lxr-Dependent Pathways In The Dorsal Root Ganglia In Models Of Obesity, Nadia Elshareif

Master's Theses

Peripheral Neuropathy is characterized as injury to the peripheral nervous system. In terms of sensory neuropathy, metabolic syndrome is involved in chronic inflammation and ER stress pathways induced by lipid overload, leading to the structural and physiological damage of afferent sensory nerves. To protect the cell from lipid-induced ER stress, Liver X Receptors (LXRs) are sterol-activated nuclear transcription factors that are of particular interest due to its role in the regulation of lipid homeostasis, membrane phospholipids, and inflammation in metabolic tissues. Their role in the peripheral nervous system remains to be elucidated. LPCAT3, regulated by LXRs, has been shown to …

The Utilization And Optimization Of Omics Trait Prediction Models Within And Across Diverse Populations, Ashley Mulford

Master's Theses

Most cancer chemotherapeutic agents are ineffective in a subset of patients; thus, it is important to consider the role of genetic variation in drug response. Lymphoblastoid cell lines (LCLs) derived from 1000 Genomes Project populations of diverse ancestries are a useful model for determining how genetic factors impact variation in cytotoxicity. In our study, LCLs from three 1000 Genomes Project populations of diverse ancestries were previously treated with increasing concentrations of eight chemotherapeutic drugs and cell growth inhibition was measured at each dose with half-maximal inhibitory concentration (IC50) or area under the dose-response curve (AUC) as our phenotype for each …

Assessing The Role Of The Interaction Between Polyamines And Heparan Sulfate In Coxsackievirus B3 Viral Infection, Oreoluwa Omoba

Master's Theses

Non-polioenteroviruses are highly infectious viruses that typically cause mild asymptomatic cases but can cause severe disease such as aseptic meningitis and dilated cardiomyopathy. Coxsackievirus B3 (CVB3) is a member of this group and is prevalent in the United States and Eastern Asia. Viruses like CVB3 rely on the host cell for many molecules in order to replicate. One such set of molecules is the polyamines. Polyamines are small, positively charged molecules that play a role in a multitude of cellular processes including RNA/DNA stabilization, gene expression, translation, and regulating membrane fluidity. Previous studies have shown CVB3 relies on polyamines for …