Digital Commons Network^™

The Effects Of Stress And Alcohol On Hiv-1 Latency, Sarah Ilene Talley

Master's Theses

A major barrier to HIV eradication is the persistence of latent viral reservoirs that exist despite antiretroviral therapy. In order to develop effective therapeutics, a comprehensive understanding of latency and factors driving the formation of the latent reservoir is needed. As stress and alcohol are common comorbidities associated with HIV infection, the goal of this research was to determine how stress and alcohol could affect HIV-latency. Specifically, we hypothesized that the ability of latent proviruses to be reactivated by “shock and kill” approaches would be altered by glucocorticoid and ethanol treatments, and prolonged ethanol exposure would affect the size of …

Characterization Of Exopolysaccharide (Eps) Produced By Bacillus Subtilis Mutants, Alexander Argianas

Master's Theses

Bacillus subtilis is a probiotic bacterium that can protect against the murine-

specific attaching and effacing (A/E) pathogen Citrobacter rodentium. C. rodentium displays many features observed with the human pathogen enteropathogenic E. coli (EPEC). Protection by B. subtilis is mediated by exopolysaccharide (EPS), which is encoded by the epsA-O operon that contains 15 genes. Disruption of one of these genes, epsH, which appears to encode a putative glycosyltransferase, leads to the loss of protective capacity by B. subtilis and by EPS. EPS is a major component of the B. subtilis biofilm and the ΔepsH mutant is unable to form a …

Role Of Heat Shock Proteins In Maintenance Of The Gut Barrier Following Burn Injury, Abigail Rhea Cannon

Master's Theses

Introduction: Burn injury remains a prominent clinical problem. Patients suffering from burns often succumb to secondary infectious complications leading to sepsis and widespread tissue injury ending in Multiple Organ Dysfunction. However, the mechanism behind the onset of these extraneous symptoms following burn injury is not fully understood. The integrity of the gut barrier is of critical importance as it harbors the largest bacterial reservoir in the body. Following burn injury, we observed a breakdown of the gut barrier resulting in increased gut leakiness and bacterial translocation. Under homeostatic conditions, heat shock proteins (HSPs) stabilize tight junction proteins. Particularly, HSP72 is …

Tcr Modifications To Enhance Expression, Chain Pairing, And Antigen Recognition For Adoptive T Cell Transfer, Kendra Foley

Master's Theses

T cell receptor (TCR) gene modified T cells for adoptive T cell transfer therapy have been shown to have clinical success in treating melanoma and other malignancies by redirecting the specificity of peripheral blood lymphocytes (PBL) to recognize tumor and/or viral associated antigens of choice. One of the challenges in using TCR gene modified T cells is the potential mispairing between endogenous and introduced alpha and beta TCR chains, allowing for unwanted off-target reactivity, autoimmunity, or impaired therapeutic efficacy. One approach to augment proper TCR chain pairing and to enhance T cell function involves the modification of the introduced TCR …

Combating Hiv/Aids In Marginalized Communities: Papua And West Papua Provinces, Indonesia, Bani Cheema

Master's Theses

My study focuses on foreign aid and local initiatives for HIV/AIDS prevention in eastern Indonesia using the provinces of Papua and West Papua as a case study. The two provinces are home to indigenous tribal groups that are socioeconomically marginalized and most affected by the epidemic. My research investigates behavior change communication as a principal strategy undertaken by multiple organizations for HIV/AIDS prevention in this region. I take a qualitative approach by examining the effectiveness of this strategy in local communities and by revealing social and cultural barriers that impede success. Obstacles that negatively impact prevention efforts include structural violence, …

Gene Expression Analysis Of Bovine Peripheral Blood Mononuclear Cells In Response To Adenovirus-Vectored Foot-And-Mouth Disease Vaccine, Frank Zappulla

Master's Theses

Foot-and-mouth disease virus (FMDV) gives rise to a highly contagious and economically important disease of cloven-hooved animals. Vaccination reduces the economic impact by inducing serotype-specific protection. Recently, a replication-defective adenovirus-vectored foot-and-mouth disease (FMD) subunit vaccine was developed and licensed. Serum virus neutralization (SVN) titer ≥1.5 to FMDV is the best predictor of vaccine-induced protection. However, protection does not always correlate with the presence of neutralizing antibodies. For example, some animals with high SVN titer develop signs of disease, and conversely, some animals with negligible SVN titer are protected. Categorizing cattle on the parameters of seroconversion and protection status yields four …

Trophic Cascade Effects Of Deer Overabundance On Connecticut's Native Vegetation And Small Mammal Populations, Megan A. Floyd

Master's Theses

No abstract provided.

Role Of Msaa Gene In Regulation Of The Msaabcr Operon And Biofilm Development In Staphylococcus Aureus, Ahmed Alzuway

Master's Theses

Staphylococcus aureus is an important human pathogen that causes wide variety of diseases ranging from chronic biofilm associated infection to acute life threatening infection such as bacteremia, pneumonia, osteomyelitis, or endocarditis, despite the progress with antibiotics used in the treatment of bacterial infections. Furthermore, increased use of prosthetic and indwelling devices in modern medical practices has led to increased infections due to S. aureus. Treating S. aureus infections have become difficult owing to its ability to resist most of the antibiotics; this problem is further exacerbated by ability of MRSA strains to form biofilms. Emergence of community-acquired methicillin resistance …

Beef Export Price Response To Sanitary Status And Traceability Systems: Implications For Paraguay, Silvana Careaga

Master's Theses

Global beef markets are highly influenced by sanitary issues, mainly related to animal health and food safety, which have been determining levels and trends in global beef trade. Food safety issues affecting human health such as bovine spongiform encephalopathy (BSE) associated with Creutzfeldt-Jacob Disease (vCJD) in humans, and other diseases affecting production such as foot and mouth disease (FMD) has encouraged putting these issues to beef trade attention. A way to address these concerns and comply with international standards is the use of traceability systems in order to quickly identify hazard sources and assure beef quality and safety. The goal …

Cayucos Community Health Plan, Jennifer Joyce Franich

Master's Theses

Recent, mounting research shows that chronic disease, the leading causes of death and primary driver of health care costs, cannot be effectively addressed through education or preventative health alone. A physical environment that promotes health—through access to healthy food, opportunities for physical activity, quality housing, transportation options, and safe schools—is an integral part of making our communities healthier. This research and accompanying Healthy Community Plans will serve as a way for the County to begin looking in-depth at the ways the built environment (our streets, parks, and neighborhoods) contribute or detract from the health of the community. Though the creation …

Oceano Community Health Plan, Eugene Phillip Brennan

Master's Theses

ABSTRACT

Oceano Community Health Plan

Phillip Brennan

Recent, mounting research shows that chronic disease, the leading causes of death and primary driver of health care costs, cannot be effectively addressed through education or preventative health alone. A physical environment that promotes health—through access to healthy food, opportunities for physical activity, quality housing, transportation options, and safe schools—is an integral part of making our communities healthier. This research and accompanying Healthy Community Plans will serve as a way for the County to begin looking in-depth at the ways the built environment (our streets, parks, and neighborhoods) contribute or detract from the …

Can Community Water Projects Combat Child Diarrhea? Results From The Solomon Islands, Thomas G. Sackman

Master's Theses

There is a vast amount of existing literature that has empirically scrutinized whether or not community water projects have the ability to mitigate diarrheal disease. A strong and persistent belief thinks that community water projects do have the means, however, over the decades empirical work commonly finds this to simply not be true. This study expands the research question to the Solomon Islands. The research tests the hypothesis using a differences-in-differences identification strategy by utilizing the government’s staggered timing rollout of community water subprojects with whether or not a village received a community water subproject to test for a program …

Evaluation Of A Theory-Based Farm To School Program, Rebecca Kent Bishop

Master's Theses

Fruit and vegetable (FV) intakes in MS children remain lower than recommended. Theory-based, multi-component nutrition education interventions like Farm to School (F2S) target FV consumption and utilize such interventions to improve children’s dietary behaviors by incorporating nutrition and agriculture education. The current study was designed with Theory of Planned Behavior (TPB) as its framework. An intervention was implemented to enhance beliefs, norms, and self-efficacy regarding FV, thus potentially increasing the intention to consume more FV. The research objectives of this study are to describe changes over the course of the intervention in (1) nutrition knowledge; (2) norms, beliefs, and self-efficacy …

A Paleopathological Analysis Of The Moran French Colonial Cemetery, Jennifer Lynn Funkhouser

Master's Theses

This research examines the health experiences of early eighteenth-century European immigrants to the Mississippi Gulf Coast. Most were low-status individuals forcibly expulsed from France and brought to Biloxi to colonize Louisiana. Historical records report the immigration effort was poorly provisioned and that large numbers died from malnutrition and disease soon after arrival. The remains of 30 adults, presumably colonists from this period based on collagen dating and grave goods, have been recovered at the Moran site (22HR511). DNA analysis (n = 8) suggests all are European. Most are males, and only one lived past age 40. Estimated mean height is …

Benzathine Penicillin G For The Prevention Of Rheumatic Fever And Rheumatic Heart Disease In The Developing World: A Global Survey Of The Quality And Quantity Of Supply, Stephen B. Marko

Master's Theses

No abstract provided.

Efficacy Of Plant Compounds For Reducing Foodborne Pathogens On Fresh Produce, Tyler E. Mattson

Master's Theses

Four plant derived compounds, namely carvacrol (CAR), trans-cinnamaldehyde (TC), eugenol (EUG) and β-resorcylic acid (BR) were investigated as wash treatments for reducing Salmonella spp. on tomatoes. In addition, the efficacy of TC and CAR in inactivating E. coli O157:H7 and L. monocytogenes on iceberg lettuce in the presence and absence of 10% organic matter was studied. Results suggest that CAR, TC, EUG and BR were effective in rapidly reducing large populations of Salmonella spp. on tomatoes when used in wash water, and CAR rapidly decreased E. coli O157: H7 and L. monocytogenes populations on lettuce even in …

Identification Of Clinical Markers That Predict The Outcomes Of Methicillin-Resistant Staphylococcus Aureus Infections And Identification Of Synergistic Antibiotic Combinations For The Treatment Of These Infections, Virginia Long

Master's Theses

Methicillin-resistant Staphylococcus aureus(MRSA) is a prominent nosocomial infection. Vancomycin is the mainstay of treatment, but it has a high rate of failure. Combination therapies can be used, but synergy is often not tested in clinical laboratories. Physicians are at a disadvantage because they cannot predict MRSA outcomes. The first aim of the study was to identify clinical and demographic markers that could predict infection recurrence or prolonged bacteremia. This was accomplished using retrospective chart review and statistical analysis. The second aim of the study was to identify which combinations, of those commonly used for MRSA treatment at Loyola, demonstrate the …

Role Of The Intestinal Microbiota In Gut Barrier Dysfunction Following Burn Injury, Zachary Earley

Master's Theses

Burn injury represents a major medical problem with half a million cases requiring medical attention and 4,000 deaths reported annually. Sepsis and multiple organ failure remain the leading causes of death following injury, and may be brought on by bacterial infections or toxins. The gastrointestinal tract contains approx. 100 trillion microbes; therefore, the indigenous commensal microbiota may play a role in leading to these complications or infections in burn patients. The overall objective of this project is to identify a potential mechanism whereby changes in gut bacteria may lead to intestinal inflammation or bacterial translocation--key factors which may lead to …

Characterizing The Mechanisms By Which Community Associated Methicillin-Resistant Staphylococcus Aureus Influences Keratinocyte Innate Immune Responses During Recurrent Infection, Ashley Lynn Larm

Master's Theses

Community associated–methicillin resistant Staphylococcus aureus (CA–MRSA) infection has become a major health concern. In human epidermal keratinocytes, S. aureus is mainly recognized through toll–like receptor 2 (TLR2) and its co–receptor, CD14. We hypothesize that CA–MRSA isolates cause recurrent infections by interrupting TLR2–mediated inflammation in keratinocytes. Recurrent CA–MRSA bacterial culture supernatant exposure to keratinocytes in vitro resulted in significant decreases in pro and anti–inflammatory cytokine and HMGB1 secretion from keratinocytes as assessed by ELISAs. Recurrent CA–MRSA live infection did not result in significant changes in cytokine or HMGB1 secretion, surface receptor expression, or NFκB activation post infection as assessed by ELISA …

Characterization Of The Female Urinary Microbiota And Their Association With The Female Bladder Uroepithelium, Evann Elizabeth Hilt

Master's Theses

The current clinical dogma assumes that urine is sterile in the absence of clinically relevant infection. However, recent evidence has demonstrated the existence of a female urinary microbiota in women with and without lower urinary tract symptoms. With the knowledge that the lower urinary tract possesses its own unique microbiota, I hypothesize that certain bacterial species of the female microbiota may be the cause or play a role in lower urinary tract syndromes such as overactive bladder syndrome (OAB). About 40-50% of OAB patients do not respond to conventional anti-muscarinic and beta-3 adrenergic agonist drug treatment. One possible explanation for …

An Investigation Of The Role Of Vibrio Vulnificus Rbda And Vibrio Parahaemolyticus Sypa In Biofilm Formation, Cecilia Thompson

Master's Theses

Vibrio vulnificus and Vibrio parahaemolyticus are both bacteria that cause human infection. V. vulnificus has a polysaccharide locus, rbd, responsible for bacterial aggregation, a form of biofilm. This locus is conserved in V. parahaemolyticus and in the non-pathogen V. fischeri.

In V. fischeri, the polysaccharide locus, syp, has been extensively characterized and shown to be important for biofilm formation. In V. fischeri, the first gene, sypA, is critical for biofilm formation. V. fischeri biofilm-proficient strains form wrinkled colonies. In contrast, sypA mutants form smooth colonies, indicating a lack of biofilm formation.

To understand the function of RbdA and SypAVP, proteins …

Functional 3-D Cellulose & Nitrocellulose Paper-Based, Multiplex Diagnostic Platforms Without Coupling Agents, Mackenzie Elizabeth Tageson

Master's Theses

The purpose of this thesis was to demonstrate device functionality of 3-D paper-based, multiplex platforms, µPADs, without the use of coupling agents between layers. Previously, these platforms were fabricated with double-sided tape and cellulose powder to try to augment proper fluid routing, but difficulties with this method occurred. An acrylic housing unit with strategically placed pressure tabs was designed to aid horizontal and vertical fluid routing through the platform, thus eliminating the inconsistencies associated with coupling agents. Channel characterization studies, a COMSOL^TM simulation, and development time studies were performed to aid device design and demonstrate device functionality.

The implementation …

The Design And Fabrication Of An Electrostatically Actuated Diaphragm With A Silicon-On-Insulator Wafer, Elizabeth L. Brooks

Master's Theses

Electrostatically actuated silicon membranes were designed, modeled, fabricated, and characterized. The intended application was for use in a microspeaker. Fabrication issues necessitated the use of thick diaphragms with a large gap between the electrodes. The devices did not function as speakers but did show actuation with a high DC voltage. Device dimensions were chosen by examining membrane mechanics, testing the processing steps required for device fabrication, and modeling with COMSOL. Several adhesives were researched to fabricate the device sidewalls, including BCB, PMMA, and TRA-Bond F112. A method for patterning PMMA through photolithography was found using a scanning electron microscope. Masks …

Analysis Of Post-Cranial Traits For The Assessment Of Biological Distance Among Temporally Distinct New World Populations Of African Descent, Claire Marie Dansereau

Master's Theses

This investigation examines change in post-cranial osteometric markers in temporally distinct New World populations of African ancestry: Newton Plantation series (17th-19th century Barbados; N = 16); two subsamples from the Hamann-Todd (HT) series (U.S.) based on birth year, 1865-1900 (N = 66) and 1901-1915 (N = 62); and the Forensic Databank Collection (FDB) (20th century U.S.; N = 137). It was anticipated that individuals from Newton would exhibit less admixture than those from H-T or FDB. They were also predicted to demonstrate greater skeletal robusticity due to mechanical loading. Additionally, it was expected that stature would increase over time due …

Comparative Analysis Of Antiviral Responses In Mouse Embryonic Stem Cells And In Mesenchymal Stem Cells, Jundi Wang

Master's Theses

Embryonic stem cells (ESCs) are cells that have unlimited capacity for selfrenewal and differentiation. These properties make ESCs a great cell source for application in regenerative medicine. When used for cell therapy, ESC-derived cells could be placed in a wounded area that is likely to be exposed to various pathogens. However, it is not well-understood whether ESCs and ESC-derived cells have active antiviral responses against infectious agents from the environment. To answer this important question, I comparatively analyzed the antiviral responses of ESCs and mesenchymal stem cells (MSCs, C3H10Tl/2 cell line) to infectious agents. Using the expression of type I …

Molecular Detection Of Tick-Borne Pathogens Associated With Ixodid Tick Species Infesting Migratory Songbirds And Ruminants, Nabanita Mukherjee

Master's Theses

Ticks are hematophagous arthropods and capable of inoculating various infectious agents to their vertebrate hosts. Ticks attached to birds and ruminants are capable of carrying tick-transmitted microorganisms. In our study, the prevalence of tick-borne pathogens was examined in ticks infesting migratory songbirds and Pakistani ruminants. During spring migration seasons, ticks were removed from northward-migrating songbirds in Louisiana and identified as Amblyomma Jongirostre, Amblyomma nodosum, Amb/yomma ca/caratum, Amblyomma maculatum, and Haemaphysalis species, all are considered as Neotropical ticks. Ticks removed from ruminants were identified as Hya/omma anatolicum, Hyalomma dromedarii, Rhipicephalus microplus, and Rhipicephalus sanguineus. A total of 112 and 72 bacterial …

A Spatial Analysis Of West Nile Virus In Texas, 2012, Allison G. Bradshaw

Master's Theses

The outbreak of West Nile Virus (WNV) in 2012 included over 1,880 reported cases in the state of Texas, and was a dramatic increase from the disease prevalence of previous years. Based on the framework of human ecology triangle, this investigation sought to test the validity of previously identified factors of West Nile Virus to this year’s outbreak and investigate the broad range of incidence rates across zip codes in Texas. A selection of demographic, environmental, and weather-related risk factors was included in the analysis in order to examine areas of high and low incidence across a varied landscape.

A …

Alteration Of Biological Properties Of Bacterial Lipids By Calcium Hydroxide Treatment, Sharefa Al-Asfour

Master's Theses

No abstract provided.

Escherichia Coli Strain Diversity In Humans: Effects Of Sampling Effort And Methodology, Emily R. Neal

Master's Theses

Studies investigating Escherichia coli strain diversity and demographics in human hosts are frequently inconsistent regarding sampling effort and methodology while current strain typing methods are often expensive or laborious. To rectify these inconsistencies, sampling effort was investigated by comparing the diversity of 15-isolate collections to 100-isolate collections from 3 human subjects. Temporal variation in E. coli strain diversity was also studied by collecting 15 isolates once every 6 months. Additionally, strain identification and diversity collected by different sampling methods (fecal swabs vs. anal swabs collected at different times around defecation) were compared to identify any inherent biases in sampling method. …

The Role Of Self-Efficacy In Increasing Food Security Among Participants Of A New Food Pantry Model In Hartford, Ct, Angela G. Colantonio

Master's Theses

Background: Food insecurity is defined as not having the resources to obtain enough safe, nutritionally adequate food in socially acceptable ways to support an active, healthy life. A new approach to emergency food assistance is needed, and researchers have encouraged the exploration of empowerment, self-efficacy, and goal setting as a means of better understanding and preventing food insecurity. Objective: The study aim is to examine the association between food insecurity and self-efficacy, and evaluate the ability of a new food pantry model (Freshplace) to increase the food security and self-efficacy of members. Methods: A randomized control trial comparing Freshplace to …