Digital Commons Network^™

Cellular Consequences Of Lysosomal Rupture Induced By Alpha Synuclein, Zachary Carpenter Green

Master's Theses

Misfolded, toxic forms of amyloid alpha synuclein (α-syn) have been implicated in Parkinson’s disease (PD) pathology and have been shown to spread throughout the brain in a prion-like manner, explaining the progressive loss of neurons in PD. The mechanisms of spread and cell death are unknown, however, evidence from previous studies suggests that neurons may undergo apoptosis due to the uptake of exogenous α-syn aggregate through the endocytic pathway and subsequent lysosomal rupture.

This study aimed to corroborate previous evidence of α-syn induced lysosomal rupture and investigate the cellular repercussions of rupture with respect to cell death and α-syn transfer. …

Estrogen Regulation Of Mir-181a Stability In Neurons, Chun Kim

Master's Theses

Estrogens are an important class of steroid hormones involved in many cellular signaling processes. Levels of estrogens fluctuate across the lifespan suggesting that they target intracellular mediators, such as microRNAs, to fine-tune homeostatic cellular processes. Previous work in our lab demonstrated that 17β-estradiol (E2), the predominant circulating estrogen, stabilized miR-181a in a neuronal cell line derived from rat hypothalamus. These results lead to the hypothesis that E2 regulated miR-181a stability by altering the pool of endogenous RNA and by recruiting PNRC2 away from the miRNA degradation machinery. By utilizing miRNA degradation assays and immunostaining techniques, it was determined that the …

Interfering In A Non-Interference Policy: Defining Star Trek's Prime Directive, Andrew Steele

Master's Theses

The aim of this thesis is to provide a comprehensive definition of the Prime Directive, a concept that was developed in Star Trek: The Original Series, as well as provide a non-fictionalized version of the edict.

The Prime Directive is an edict that governs Starfleet not to interfere with a society’s development.

The first main section will discuss in further detail why The Original Series account of the Prime Directive was selected. Furthermore, it will discuss how members of academia interpret the Prime Directive.

The second main section will be an episode-by-episode analysis of each Prime Directive centered episode to …

Psychological Predictors Of Community Violence Exposure In Ethnic Minority Male Adolescents, Amanda Burnside

Master's Theses

Urban, minority males are disproportionately impacted by community violence exposure (ECV). However, person-based analyses have demonstrated variability in rates of ECV, suggesting that demographic risk factors do not always result in increased ECV, and it may be important to examine the utility of psychological factors in this relationship. Research suggests that depressive symptoms may actually exacerbate the risk of ECV. The current study examines the effect of internalizing symptoms on future ECV. Data were derived from a larger longitudinal study of adolescents who had committed a criminal offense. This subset of 184 participants ranged in age from 14-18. Results indicated …

Parenting-Related Stress, Parental Distress, And Youth Health-Related Quality Of Life In Families Of Youth With Spina Bifida: Parenting Behaviors As Mediators, Colleen F. Bechtel

Master's Theses

Research has shown that youth with spina bifida (SB) have poorer psychosocial outcomes, including health-related quality of life (HRQOL), compared to typically developing youth. Demographic and illness-severity factors that may affect HRQOL have been identified, but modifiable factors affecting HRQOL have not yet been identified in this population. Potential modifiable factors include parent factors. In fact, in other pediatric populations, parent factors have been found to impact HRQOL above and beyond illness-severity. This impact may be especially salient for youth with SB, as these youth are more socially isolated and depend on parents for both medical and non-medical caregiving needs. …

In Plain Sight: How Social Images In Education Shape The Social Identity Of Black Students, Kandyce Cabacar

Master's Theses

This thesis will postulate social identity development in Black students is shaped by their school experience. Reviewing how racial grouping is a critical factor in how we engage with others socially, this study will curate the variables of society that assembles the Black image. Social construction influenced by media messages and the neighborhoods that determine our respective schools will also be analyzed. By borrowing from the research of social psychology and contributions of sociologists on race relations, this thesis will demonstrate how expectations influenced by racial stereotypes of Black students create a correlation to social identity. These findings are discussed …

Haitian Representation In The Media: A Comparative Analysis Between An African American Owned Newspaper And A Mainstream Newspaper, Stephanie Jean-Baptiste

Master's Theses

Are the portrayals of Haitian immigrants in an African-American owned newspaper, different from their portrayal in a white owned newspaper? In this paper I examine newspaper articles covering Haitian immigrants and refugees. The articles selected were published in 2004 and sampled from the Chicago Tribune and the Chicago Defender. Although the journals have distinctive goals and market reach, there were similarities in their portrayal of Haitian immigrants. Both journals referred to Haitian immigrants as victims of Haiti’s failed democracy. However, each journal advertised the reception of this migrant group differently. The African American owned newspaper advertised inclusion, while the white-owned …

Down And Out: College Students Experiencing Depressive Symptoms, Jenna Eileen Ofenloch

Master's Theses

Survey research that asks respondents to report on depression often ask

respondents whether they have been diagnosed with depression by a medical

professional. But such questions underestimate depression by leaving out respondents

who are unable or unwilling to seek professional help. Thus, other studies seek to ask

respondents to report symptoms of various dimensions of depression. This is especially

important because, according to the CDC, “persons with mild depressive symptoms, as

well as those with moderate or severe depressive symptoms, reported difficulties with

work, home, and social activities related to their symptoms”. But some groups are more

prone to depression. …

Summertime Sleep And Bmi In Urban Minority Girls: Relations To Physical Activity And Executive Functions, Carolyn Rose Bates

Master's Theses

Urban minority youth, particularly females, are at high risk for increased weight gain during the summertime months, and may also experience insufficient sleep at this time. Few studies have objectively measured summertime sleep in this population or related sleep to weight gain during this season. The current study draws on a sample of 66 urban minority girls aged 10-to-14 who participated in a community-based summer day camp program promoting physical activity (PA). The study objectively characterizes sleep in this sample, both in unstructured and structured contexts. Additionally, the study examines potential pathways underlying summertime relations between sleep and weight, including …

Feminist And Gay Male Politics: New Issues For Political Collaboration And Activism, Adam Hii

Master's Theses

This project looks at political collaboration between people who self-identify as gay men

and those who self-identify as feminists. These two groups have been political aligned on numerous issues since the 1970s. The goal of this project is to see on which issues that political collaboration will continue moving forward. This research draws extensively on oral interviews conducted with gay men and feminists, as well as the current work of national advocacy organizations to see where political interest is high, and where networks already exist to foster future advocacy efforts. By focusing on these areas, this project predicts which issues …

Phosphodiesterase Inhibition As A Mechanism Of Ion Channel Modulation By Celecoxib, Christina Jean Robakowski

Master's Theses

The overall objective of this thesis work is to determine the mechanism by which celecoxib modulates Kv7 potassium channels in vascular smooth muscle cells. The central hypothesis of the project is that celecoxib modulates Kv7 potassium channels in vascular smooth muscle cells through inhibition of phosphodiesterase 4 (PDE4, which catalyzes the breakdown of cyclic adenosine monophosphate (cAMP)), thereby activating a cAMP/protein kinase A (PKA) signaling pathway. The aims of this thesis targeted different points in the cAMP/PKA pathway with pharmacological interventions using whole cell perforated patch clamp electrophysiology and biochemical methods.

The data suggest multiple mechanisms by which celecoxib could …

Examining The Effects Of Coping Strategies Specific To Community Violence Exposure Among African American Adolescents, Suzanna So

Master's Theses

Variability in exposure to community violence (ECV) and aggressive behaviors in African American youth from urban communities can be attributed to general coping, but these studies have been inconclusive. Recent qualitative research identified four types of coping that are specific to ECV; however, quantitative research is needed to understand the adaptiveness of these strategies. The current study examined the factor structure of a measure for ECV-specific coping strategies. The current study also assessed how ECV-specific coping was associated with ECV and externalizing behaviors. Data from the current study were derived from an archival dataset comprised of 594 African American adolescents. …

Risk And Resilience Factors Among Low-Income Latino Adolescents: The Impact On Daily Ratings Of Mood, Stephanie Andrea Torres

Master's Theses

Given that Latino adolescents endorse more negative mood when compared to their counterparts of other backgrounds (CDC, 2012), it is especially advantageous to evaluate the impact of risk and resilience factors on mood among this population. The current study uses daily diary methodology to examine the impact that daily economic stress, daily family stress, familism, and ethnic identity commitment and exploration have on daily ratings of mood among 58 (M =13.31, 47% female) low-income Latino adolescents. Results show that daily family stress was strongly linked to daily mood while familism emerged as a salient resilience factor. Contrary to predictions, ethnic …

A Bioluminescence Sensor Of Nlrp3 Inflammasome Activation, Michael Alexander Winek

Master's Theses

The innate immune system is many organisms first line of defense against pathogenic insult or tissue damage. This defense strategy is intent on restoring homeostasis upon perturbation. Upon activation of the innate immune system in humans, an oligomeric protein complex termed the “Inflammasome” forms in myeloid cells. The canonical output of activation of any subset of inflammasome is Caspase-1-mediated secretion of pro-inflammatory cytokines IL1β and IL18. Chronic or uncontrolled inflammasome activation is at the core of myriad economically burdening diseases. In many of these diseases, endogenous factors chronically engage the innate immune system. To study these diseases in in vivo, …

The Moderating Role Of Emotion Regulation On Longitudinal Associations Between Stress And Mental Health In College Students, Evan Zahniser

Master's Theses

Emotion regulation is consistently linked to subsequent wellbeing, but little research has examined the moderating role of emotion regulation in associations between mental health and other relevant factors. Patterns of gender differences in emotion regulation also remain somewhat unclear. The present study targets these gaps by examining two specific emotion regulation strategies in interaction with stress and gender in predicting internalizing symptoms among college students, a population for whom emotion regulation may be particularly important given the high-stress nature of the college transition. A large sample of students (N = 1,130) provided self-report data at three time points over their …

Preliminary Investigation Of Commercially Available Pluronics As Uv Curable 3d Printing Inks For Tissue Engineering Applications, A'Lester Cordell Allen

Master's Theses

Three-dimensional printing (3DP) emerged from simple beginnings in the field of additive manufacturing (AM) over 31 years ago as an economical technique for rapid prototyping. Now 3DP has become the premier method for fabricating materials from unique consumer products to lifesaving customized human organs. Current challenges in bioprinting center around balancing material properties such as stiffness, yield strength, and surface chemistry with non-Newtonian fluid flow to construct interconnected, porous scaffolding geometries that simulate the complex vasculature found in the extracellular matrix (ECM) of the human body needed to promote cell growth and regeneration. In this study, several copolymers of poly(ethlyene …

Solitary By Any Other Name: Silence To Segregation In American Prisons, Chelsea Van Aken

Master's Theses

This thesis examined the United States’ ability to circumvent international and constitutional law in regards to solitary confinement in American prisons. Drawing on scholarship examining inmates’ rights and inmates’ resistance movements, international human rights doctrine, United States constitutional law, activist led movements, and inmate testimony, the thesis demonstrates that the United States is able to simultaneously claim that it is meeting its human rights violations while resisting reforms to both state and federal current policy of warehousing inmates in solitary confinement for decades at a time through two strategies. First, the United States utilizes framing strategies to deny the use …

Examining The Moderating Effect Of Life Satisfaction On The Relationship Between Perceived Underemployment And Job Attitudes Among Millennials, Savannah M. Trakes

Master's Theses

Underemployment has been associated with negative attitudes and behaviors. However, little research attention has been paid to identify variables that might moderate the relationship between underemployment and job attitudes. Given that Millennials are one of the groups most susceptible to underemployment, I examined the relationship between perceived underemployment and job attitudes (i.e., job satisfaction, intent to remain) using life satisfaction as a moderator among Millennial employees. Based on survey data from 552 Millennial employees across various sectors, results showed that perceived underemployment was negatively related to job satisfaction, intent to remain, and life satisfaction. Inconsistent with the prediction, life satisfaction …

Effects Of Zoledronate During Simulated Weightlessness On Bone Structure And Mechanics, Ryan T. Scott

Master's Theses

Spaceflight causes astronauts to experience an unbalanced level of bone remodeling favoring resorption. The combination of an antiresorptive drug and weightlessness may have negative effects on the mechanical and structural properties of bone by increasing elastic stiffness but diminishing plasticity. At 16 weeks of age, skeletally postpubescent male mice (n = 32, C57BL/6) were evenly divided and subcutaneously given either zoledronate (45 µg/kg) or vehicle (saline, same volume). Three days postinjection, half of each group were divided to undergo 3 weeks of hindlimb unloading or act as a control undergoing normal ambulation (n = 8 for each of the 4 …

Good Grief, Alexandra Deabler

Master's Theses

Good Grief is a novel that follows one family as they move through the five stages of grief. The purpose of the creative work is to focus on the complicated relationships of a blended family, and further complicate that with the added pressure of mourning a loved one, while still fulfilling familial and societal obligations. It is a meditation on the different ways people handle emotional trauma. Grief is a universal concept, but often mistreated or misunderstood. Grief, as a process, takes several different forms, some of which—expressing pain through joviality or sensuality—are shrouded in shame. My thesis challenges the …

Exposure Assessment Of Asthma And Modeling Of Pm2.5 During The 2007 Southern California Wildfires, Areana Flores

Master's Theses

A three-year study (1 Jan 07 to 31 Dec 09) was conducted for Los Angeles and Riverside counties to validate current findings on impacts of wildfires on respiratory health. A dataset developed from multiple sources containing daily rates of air pollution (O3, NO2, CO, and PM2.5) and meteorological variables (temperature, dew point, wind speed, and inversion height) was correlated with asthma emergency department (ED) visits. A second correlation was calculated for a modified dataset that excludes all episodes of wildfire events within the study period. The difference in correlations between both datasets was computed. PM2.5 was positively associated with asthma …

Evaluation Of A Silica Hydride-Based Undecynoic Acid Stationary Phase For High Performance Liquid Chromatography, Veena Menon Kozhikote

Master's Theses

Undecynoic acid (UDA) attached to a silica hydride surface has been found to exhibit weak cationic exchange properties and an aqueous normal phase (ANP) type of retention. ANP is a mechanism similar to normal phase chromatographic retention, except for the use of a polar solvent such as water in the mobile phase. In ANP, the retention of a polar molecule is increased with a higher concentration of the non-polar mobile phase solvent (usually acetonitrile). Non-polar molecules exhibit retention behavior as in reverse phase (RP) chromatography. The goal of this research was to characterize the chromatographic retention pattern of an undecynoic …

Structure-Function Relationship Between Quercetin, Its Methylated Derivatives And Cytotoxicity In Triple Negative Breast Cancer Cells, Jyoti Phatak

Master's Theses

Quercetin is a member of the flavonoid family, found in fruits and vegetables, and has

shown cytotoxic effects on many cancers, including breast cancer. Quercetin’s methylated derivatives, 3-O-methyl quercetin, pentamethyl quercetin, isorhamnetin, and tamarixetin, differ in their substitution patterns of the methyl capping at the 5-hydroxyl groups. Cell death assays performed on MDA-MB-231 cells showed increased cytotoxicity with quercetin and all its methylated derivatives, except pentamethyl quercetin, after 72 hours of treatment. Loss of the mitochondrial outer membrane potential (MOMP) was seen using a JC-1 assay after 15 min incubation with compounds, except for pentamethyl quercetin. We saw no change …

Deconstructing Exploitative Systems And Restoring A Balanced Biosphere: An Ecofeminist Posthumanist Reading Of Jane Smiley's A Thousand Acres And Barbara Kingsolver's Prodigal Summer, Aubrey A. Laughlin

Master's Theses

Although many scholarly articles have provided ecofeminist insights and

critiques of A Thousand Acres that connect the abuse of women to the abuse of the land, few have dealt specifically with the link between the treatment of women and the treatment of nonhuman animals. In the first chapter of this paper, I argue that associations between women and nonhuman animals in A Thousand Acres sustain the constructed reality of patriarchal communities. Similarly, Prodigal Summer’s narratives center around females and nonhuman animals, but also provide a broader focus that emphasizes the interconnectivity of the entire biotic pyramid and optimistically holds that …

Exploring The Relationship Between Commuting Stress, Job Strain And Safety Behaviors While Commuting: The Dual Role Of Work-Related Rumination, Katrina A. Burch

Master's Theses

For those who commute to and from work on a daily basis, this is an activity that requires attention to both what one is doing and the environment, in order for the commute to be done safely. Although research has shown that work can spill over into home and other non-work domains, very little attention has been paid to the impact that work may have on the transition time between one’s work and home domains. The present study sought to examine the impact of daily commuting stress and end-of-day job strain on the safety of one’s commute through the experience …

Learning Delays In A Mouse Model Of Autism Spectrum Disorder, Amanda R. Rendall

Master's Theses

Autism Spectrum Disorder (ASD) is a heterogeneous neurodevelopmental disorder with core symptoms of atypical social interactions and repetitive behaviors. It has also been reported that individuals with ASD have difficulty with multisensory integration, and this may disrupt higher-order cognitive abilities such as learning and social communication. Impairments in the integration of sensory information could in turn reflect diminished cross-modal white matter connectivity. Moreover, the genetic contribution in ASD appears to be strong, with heritability estimates as high as 90%. However, no single gene has been identified, and over 100 risk genes have been reported. One of these genes -- contactin-associated-like-protein …

Revisiting Community Water Fluoridation, Jessica Marie Seyfried

Master's Theses

There is a bourgeoning controversy unknown to many surrounding one of the CDC’s top ten public health achievements of the 20^th century. After more than 50 years of implementation, community water fluoridation may not have achieved the declines in caries rates we once thought or hoped for, especially in light of alternative approaches to preventing caries including but not limited to other sources of fluoride supplementation, changes in diet, and tooth brushing behavior.

In fact, a survey of past research highlights the fact that this policy decision may be based on out of date and/or a lack of research …

“How Do You Define Yourself?” Mobilizing Leadership In The Graduate Employee Union Movement, Kathleen Ragon

Master's Theses

Social movement scholars agree that leadership plays a crucial role in the emergence, structure, and outcomes of social movement organizations (SMOs) and is thus an indispensable area of research. The vast majority of theories of leadership to date have been developed in contexts where participants’ movement identities are relatively stable (e.g., gender, sexual, or occupational) and engagement in the site of contestation will be long-term (e.g., workplace or nation state). However, the recent proliferation of graduate student employee unions poses a challenge to this literature because graduate students’ employment is by its very nature temporary and variable; graduate assistantship appointments …

The Role Of The State, Multinational Oil Companies, International Law & The International Community: Intersection Of Human Rights & Environmental Degradation Climate Change In The 21st Century Caused By Traditional Extractive Practices, The Amazon Rainforest, Indigenous People And Universal Jurisdiction To Resolve The Accountability Issue, Marcela Cabrera Luna

Master's Theses

Local, national and international conventions that protect indigenous sovereignty and their territories, where many of the resources are extracted from by multinational corporations (MNCs) particularly oil, the number one commodity of the world and cause of climate change, continue to be jeopardized because of the lack of a clear international legal framework that can protect them and potentially hold multinationals accountable for their actions. These practices are causing not only environmental issues to the indigenous and surrounding communities, but climate change is in fact, the real human rights issue of the 21^st century and it affects everyone. By using …

The Tunisian Revolution: Empire And The Power Of The Multitude, Caroline A. Burns

Master's Theses

The self-immolation of Mohammed Bouazizi ignited the revolution that would oust Tunisian dictator Ben Ali in 2011. The momentum of the revolution in Tunisia spread ideas, tactics, and revolutionary chants across borders to various parts of the globe. The speed and intensity of the revolution dominated the attention of the unsuspecting global community. In order to understand the conditions under which this revolution transpired, I use Michael Hardt and Antonio Negri's theory of Empire to show how the contemporary global system functions. Through the historical development of Tunisia and concurrent rise of Empire emerges "the multitude," the heterogeneous manifestation of …