Digital Commons Network^™

The Effect Of Focused Ultrasound On Altering The Diameter Class Of Nerve Fibers Contributing To A Compound Evoked Potential, Analyzed Using A Perturbative Decomposition Technique., Megan Wurden

Master's Theses

Peripheral neuropathies are disorders that involve the damage of peripheral nerve fibers, affecting the ability of different parts of the body to communicate. A differentiating factor in diagnosis between various clinical conditions can be which size class of nerve fibers are affected. A nerve conduction velocity test can be used to assess the viability of the nerve but is a single-parameter test and gives no information about the population characteristics of the remaining active fibers. A method developed and previously reported by Szlavik (2016) utilizes a mathematical perturbed decomposition to determine the normalized frequency of each size class of fiber …

Early Wildfire Detection With Line Sensors, Virginia Yan

Master's Theses

Over the last few years, wildfires have become more devastating to communities as the fires are inevitably destructive to many homes, businesses, and ecosystems. Frequent wildfires also pose a significant threat to power grids and nearby residents as they can damage transmission lines and other electrical equipment, which in turn can cause major power shutdowns. Especially in western U.S., severe drought conditions and weather variability cause residents to become more vulnerable to wildfire disasters as their safety is threatened. We are incompetent to control the wildfires effectively despite existing advanced technologies. Hence, an algorithm based on energy conservation and heat …

Development Of A Robust Methodology To Obtain And Assess Myogenic Precursor Cells For Their Use In Regenerative Therapies, Ricardo Lasa

Master's Theses

Peripheral arterial occlusive disease (PAOD) is characterized by buildup of atherosclerotic plaque in peripheral arteries that leads to an occlusion that can interrupt the supply of blood to the peripheral tissue, causing downstream tissue ischemia/hypoxia. PAOD is estimated to affect over 200 million patients worldwide. Current surgical revascularization treatments can be effective in about half of the patient population, leading to a significant number of patients with no treatment options beyond pharmacological intervention and lifestyle modification. The decrease in blood flow downstream of the occlusion leads to increased blood pressure gradient in the microvasculature, specifically in vessels that connect arterial …

Development Of Novel Hardware And Software For Wind Turbine Condition Monitoring, Ryan A. Zhan

Master's Theses

With the increased use of wind turbines as sources of energy, maintenance of these devices becomes more and more important. Utility scale wind turbines can be time consuming and expensive to repair so an intelligent method of monitoring these devices is important. Commercial solutions for condition monitoring exist but are expensive and can be difficult to implement. In this project a novel condition monitoring system is developed. The priority of this system, dubbed the LifeLine, is to provide reliable condition monitoring through an easy-to-install and low-cost system. This system utilizes a microcontroller to collect acceleration data to detect imbalances on …

Effect Of Simulated Storage And Distribution On Listeria Innocua Growth In Non-Traditional Salad Ingredients, Emma L. Sandquist

Master's Theses

The fresh-cut produce industry has seen expansive growth in recent years, to meet consumer demand ready-to-eat (RTE) salads have included the use of non-traditional ingredients. Uncommon ingredients include beet greens, kale, broccoli stalk, and Brussels sprouts, since these ingredients have not historically been consumed raw, potential food safety issues should be reassessed. Current processing technologies include produce washes that can reduce microbial levels but do not eradicate all populations. The lack of a kill step in produce processing emphasizes the need to minimize pathogen contamination during production and growth during a product’s shelf life. Listeria monocytogenes, a leading cause …

Estimating Food Waste Due To Food Safety Recalls And Investigating Ways To Minimize Negative Impacts, Mykayla Latronica

Master's Theses

For years the issue of food waste has been recognized and quantified; however, food safety issues often go unrecognized as a source of food waste. One objective of this research is to estimate quantities and monetary value of fruits and vegetables implicated in food safety recalls, and thus wasted. Using publicly available data we identified all recalls involving vegetable or fruit commodities contaminated with Listeria monocytogenes, pathogenic E. coli, or Salmonella during 2015-2018. When quantities were provided, monetary value of recalled product was calculated using USDA ERS 2016 average retail prices. Although data limitations only allowed analysis of …

Through The Eyes Of Community Educators: Place-Based Learning, Equitable Access, And Covid-19 Impacts On Outdoor Environmental Education In The San Francisco Bay Area, Alycia Ellington

Master's Theses

Not all children receive adequate education, nor do they have the opportunity to go beyond the four walls of a classroom for learning opportunities. Outdoor environmental education with place-based learning frameworks aims to fully immerse students in nature, while strengthening the connection between schools and communities. This study examines the outdoor environmental education programs of nine agriculture-centered community organizations in the San Francisco Bay Area. Through twenty-one key informant interviews, I assess three essential areas: 1) promotion of environmental literacy and place-based learning, 2) facilitation of equitable access to educational programs, and 3) adaptation of those programs for distance learning …

Options For Low-Cost Manufacturing And Safer Cell Therapies, Melis Keceli

Master's Theses

The proposed work aims to overcome the economical and feasibility-related limitations of the chimeric antigen receptor therapies by developing an artificial cell signaling pathway whose design transforms K562 cells into in vivo living vectors to synthesize therapeutic proteins upon engaging diseased cells in the treatment of ovarian cancer. There are various advantages of using K562 cells throughout this process. First, Food and Drug Administration approves the reinfusion of K562 cells into patients’ bodies. Second, K562 cells are more affordable than T lymphocytes, and finally, these cells can be easily manipulated with any desired genetic material and can keep the expression …

Andrews University Communication Under The Covid-19 Pandemic: Feedback Analysis Of International Students, Brice B. Eboule

Master's Theses

This research is an informative analysis of international students’ feedback on the Andrews University (AU) crisis communication during the COVID-19 pandemic in 2020. Participants were 21 years or older, on student visas, living on-campus or in student communities and had attended AU for at least 2 years. The theory informing this study is situational crisis communication theory (SCCT). The SCCT argues that the severity of the crisis defines which communicational crisis strategy will be more effective in minimizing attributions and adverse press while both maintaining and enhancing the organization's credibility. The findings of this study indicated that 84 percent of …

The Effect Of Virtual Learning On Parents During Covid-19, Sheila Reeves

Master's Theses

Parents were affected in numerous ways by the sudden shut down of schools and switch to virtual learning in March 2020 due to the COVID-19 pandemic. Some of these ways included use of technology, income, race, time, mental health, family life and parent insecurities. This study investigates parents' perspectives of some ways they were affected in regards to the virtual learning of their child(ren). All parents with children enrolled at Madison Campus Elementary School were invited to participate in the study, as they had at least one year's experience with their child(ren) in virtual learning.

Data was collected using a …

Characterizing A Novel Cocksackievirus B3 Protease Mutant And Its Response To Polyamine Depletion, Bridget Hulsebosch

Master's Theses

Enteroviruses, including Coxsackievirus B3 (CVB3), are pervasive pathogens that cause significant disease, including cardiomyopathies. Unfortunately, no treatments or vaccines are available for infected individuals. We identified the host polyamine pathway as a potential drug target, as inhibiting polyamine biosynthesis significantly reduces enterovirus replication in vitro and in vivo. Here, we show that CVB3 is sensitive to polyamine depletion through the polyamine analog diethylnorspermidine (DENSpm) which enhances polyamine catabolism through induction of polyamine acetylation. We demonstrate that CVB3 acquires resistance to DENSpm via mutation of the 2A protease, which enhances proteolytic activity in the presence of DENSpm. Resistance to DENSpm occurred …

Heteronormativity In Healthcare: Neoliberalism, Bio-Politics, And Necropolitics, Grace Avila

Master's Theses

This thesis will discuss heteronormativity in healthcare. It will take a social science and theoretical approach to acknowledge LGBTQ+ health disparities, apply theoretical approaches to why these disparities exist, and offer solutions to combat health disparities experienced by sexual and gender minorities. Neoliberalism, bio-politics, and necropolitics will be the theoretical approaches utilized to understand the basis of disparities experienced by the LGBTQ+ community. Neoliberalism will include themes of capitalism and public health policies which influence discrimination in healthcare. Bio-politics will outline how the intersection of the biological body and politics can exacerbate health disparities through the power of controlling bodies. …

Anti-Unionism And The Chicago Teachers Union, Paul Stromberg

Master's Theses

The Chicago Teachers Union (CTU) is one of the most influential political organizations in Illinois. Like other political organizations, the CTU influences policy through activism, advocacy, and endorsements. Unlike most political organizations, however, the CTU's 20,000 members are responsible for carrying out policy decisions through their roles as teachers, paraprofessionals, and clinicians in Chicago Public Schools (CPS). In response to this influence, anti-unionists have become increasingly adept at criticizing the CTU, using anti-union rhetoric to malign unionized teachers. Simultaneously, anti-unionists have utilized anti-union litigation to disable teacher unions, stripping them of guaranteed protections. Assisted by conflict theory, this study examines …

The Gendered Impact Of Droughts On Household Decision Making: Evidence From Uganda, Fiona Onyango, Andrew Hobbs

Master's Theses

Do female headed households lose/ sell more of their livestock in the event of a drought as compared to households headed by males? We address this question through an analysis of the combination of Living Standards Measurement study Integrated surveys on Agriculture dataset on Uganda using data from the period 2011-2016 and NDVI & Weather data. We carry out both OLS and fixed effects estimations. Our OLS Results are consistent with the theory of Consumption smoothing while we do not find evidence of any gendered effects from both the OLS and IV estimations.

Do Conditional Cash Transfers Increase School Enrollment? Evidence From Brazil, Jeanette L. Silvernale

Master's Theses

Conditional cash transfer programs have been widely deployed across the globe. They seek to bolster human capital by providing benefits contingent upon a variety of actions such as school attendance and regular health checkups. Bolsa Familia is the most extensive conditional cash transfer program, serving 46 million Brazilian citizens. Despite its expansive size, there have been seldom large-scale micro evaluations of the program. Limited data has resulted in small sample sizes drawn from a single point in time. Our study diverges from the rest and utilizes a novel administrative dataset with an overall sample of nearly 60 million and a …

Temperature Effects On Prevalence Of Sexually Transmitted Infections Among At-Risk Female Sex Workers In India, Rajshri Suresh

Master's Theses

Vulnerability to climate change varies depending on the baseline climate, sensitivity to given exposure, and the presence of social, political, cultural, and institutional inequalities that influence access to essential resources of livelihood, particularly among informal labor market participants. Within the risky informal labor market, the transient nature of sex work implies that sex workers suffer disproportional losses in health, income, protection, and other aspects of general well-being as a result of the effects of climate anomalies. In this paper, I investigate the effect of temperature on the prevalence of sexually transmitted infections in a high-risk population of female sex workers …

Examining Organizational Factors And Their Impact On Older Adults In Life Plan Communities, Ajla Basic

Master's Theses

Aging in America continues to reveal multi-faceted concerns for both the industry and retirees; insufficient retirement savings, loneliness, and a high influx of older adults entering the retirement scene. It is expected that by 2030, 18% of the nation's population, the baby boomers, will have turned 65 (Cohn & Taylor, 2010). This demographic reality has led to increased research seeking answers to questions which emerge about the retirement age population. The Age Well Study is a longitudinal study looking at the impact of residing in a Life Plan Community. The data is collected through self-report measures by residents in the …

Plasmodium Tlap-2 Is A Microtubule-Associated Protein With Microtubule Stabilization Properties, Grifin Berge

Master's Theses

Microtubules facilitate major cellular and morphological changes in eukaryotic cells, including the protozoan parasite Plasmodium. Microtubule-associated proteins (MAPs), only very few of which have been characterized in the malaria parasite, regulate the highly dynamic microtubules. Thioredoxin-like associated protein 2 (TLAP2) is a MAP unique to the phylum apicomplexa, which in addition to Plasmodium includes the important human pathogen Toxoplasma. Here we report on the cloning and microtubule-binding activity of TLAP2 of the rodent malaria parasite Plasmodium berghei (PbTLAP2). PbTLAP2 is coded by a single exon gene and consists of 598 amino acids. The protein contains a putative C-terminal microtubule-binding domain. …

Why Is Race A Risk Factor For Infants Born With Birth Defects?: Deconstructing The Biological Basis Of Race In Maternal-Fetal Medicine Through The Lens Of Reproductive Justice, Amrita Bhagia

Master's Theses

Several studies have shown that marginalized populations, especially those of non-white race/ethnicity, have an increased risk of having infants born with severe birth defects. Existing hypotheses from the scientific literature on the topic of birth defects have primarily suggested that these trends may be the result of differential genetic susceptibilities within certain racial groups, a theory that reifies the (currently disproven) biological basis of race. Through this thesis, I argue that the myth of the biological basis of race continues to exist within maternal-fetal medicine today, where it is used to further the narrative that the bodies of women of …

Chga-Depleted Urothelial Amp And Immune Response During Urinary Tract Infection, Theadora Jane Ceccarelli

Master's Theses

Women are 8 times more likely than men to experience a urinary tract infection (UTI) with up to 60% of women reporting a UTI in their lifetime. This significant healthcare burden is caused by the infection of uropathogenic Escherichia coli in the urinary tract. At this time, treatment is limited to antibiotic therapy, which is challenged by antibiotic-resistance. These factors make UTIs an NIH priority and are motivation for our research.CgA is a protein of the granin family co-released with catecholamines from neuroendocrine cells throughout the body. Pro-hormone convertases post-translationally cleave CgA into peptides, like catestatin, which can have antimicrobial …

The Identification Of Lxr-Dependent Pathways In The Dorsal Root Ganglia In Models Of Obesity, Nadia Elshareif

Master's Theses

Peripheral Neuropathy is characterized as injury to the peripheral nervous system. In terms of sensory neuropathy, metabolic syndrome is involved in chronic inflammation and ER stress pathways induced by lipid overload, leading to the structural and physiological damage of afferent sensory nerves. To protect the cell from lipid-induced ER stress, Liver X Receptors (LXRs) are sterol-activated nuclear transcription factors that are of particular interest due to its role in the regulation of lipid homeostasis, membrane phospholipids, and inflammation in metabolic tissues. Their role in the peripheral nervous system remains to be elucidated. LPCAT3, regulated by LXRs, has been shown to …

The Utilization And Optimization Of Omics Trait Prediction Models Within And Across Diverse Populations, Ashley Mulford

Master's Theses

Most cancer chemotherapeutic agents are ineffective in a subset of patients; thus, it is important to consider the role of genetic variation in drug response. Lymphoblastoid cell lines (LCLs) derived from 1000 Genomes Project populations of diverse ancestries are a useful model for determining how genetic factors impact variation in cytotoxicity. In our study, LCLs from three 1000 Genomes Project populations of diverse ancestries were previously treated with increasing concentrations of eight chemotherapeutic drugs and cell growth inhibition was measured at each dose with half-maximal inhibitory concentration (IC50) or area under the dose-response curve (AUC) as our phenotype for each …

Assessing The Role Of The Interaction Between Polyamines And Heparan Sulfate In Coxsackievirus B3 Viral Infection, Oreoluwa Omoba

Master's Theses

Non-polioenteroviruses are highly infectious viruses that typically cause mild asymptomatic cases but can cause severe disease such as aseptic meningitis and dilated cardiomyopathy. Coxsackievirus B3 (CVB3) is a member of this group and is prevalent in the United States and Eastern Asia. Viruses like CVB3 rely on the host cell for many molecules in order to replicate. One such set of molecules is the polyamines. Polyamines are small, positively charged molecules that play a role in a multitude of cellular processes including RNA/DNA stabilization, gene expression, translation, and regulating membrane fluidity. Previous studies have shown CVB3 relies on polyamines for …

Beta-Adrenergic Receptor Mediated Transcriptional Dysregulation In Hematopoeitc Stem And Progenitor Cells Leads To Bone Marrow Erythroid Suppresion In Multiple Myeloma Patients - Ex Vivo Investigations, Vimal Ravi Subramaniam

Master's Theses

The sympathetic nervous system (SNS) regulates our body’s involuntary response to stress or harm. Once activated, it releases hormones and neurotransmitters known as catecholamines throughout the body to mediate its recovery. The catecholamines bind to adrenergic receptors located on various cells in a process designated as adrenergic signaling to elicit this response. This process is not always to our benefit. Adverse symptoms such as anemia can result due to adrenergic signaling. Our laboratory previously showed that burn injury triggers adrenergic receptor stimulation resulting in anemia induced by diminished erythropoiesis. The development of anemia in these studies was found to be …

Characterization And Targeting Of Voltage-Gated Ion Channels In Meningioma Cell Lines, Erik White

Master's Theses

Meningiomas (MN) are primary brain tumors originating in the meningeal covering of the central nervous system. These tissues can evolve through familial inheritance (NF2, Cowden Syndrome, or Werner Syndrome) or sporadic mutations. The WHO categorizes MNs into three grades (I-III); Grade I contain benign tumors and Grade III the most aggressive. Increasing grade coincides with an increase in genetic diversity, making universal treatments difficult. MNs are typically treated with hydroxyurea (HU), but this chemotherapy agent has many adverse side effects, leading to research in less toxic options. This study uses ion channel antagonists for T- and L-type calcium channels using …

Anthropogenic Particle (Including Microplastic And Microfiber) Transport And Retention In Streams Under Varied Environmental Conditions, Elizabeth Marjorie Berg

Master's Theses

Microplastics (plastic particles <5 mm) and other anthropogenic particles (i.e., synthetic, semi-synthetic and anthropogenically modified cellulose particles < 5mm) are pollutants of concern in aquatic ecosystems worldwide. Anthropogenic particles enter food chains, adsorb harmful chemical pollutants, and are ubiquitous in aquatic ecosystems. Streams are key sites of anthropogenic particle input, retention, and transport, and empirical measurements of particle movement in lotic ecosystems are needed to inform global budgets of anthropogenic particles and microplastics. However, factors that influence anthropogenic particle retention in lotic ecosystems are poorly understood. We demonstrate how environmental characteristics affect plastic transport and retention in streams in experimental and observational studies. In our experimental study, we used particle spiraling metrics to directly measure microplastic retention in outdoor, experimental streams at Notre Dame's Linked Experimental Ecosystem Facility (ND-LEEF). We tested the impact of stream discharge (i.e., high, low), benthic biofilms (i.e., well-established biofilms, reduced biofilms post-scouring), and benthic substrate type (i.e., cobble, pea gravel, sand, mixed substrate) on retention of microplastic fibers using pulsed releases and synchronized water sample collection at 3 sites downstream. We also collected benthic surface samples for biomass and microplastic density. Samples were filtered directly (water samples) or after peroxide digestion (benthic samples), and experimental microplastic fibers were enumerated visually using a dissecting microscope. Microplastic deposition rates were significantly higher with (1) higher discharge, (2) well established benthic biofilm (as opposed to bare substrate), and (3) larger and more homogeneous substrates. The observational study examined anthropogenic particles (including microplastics and microfibers) in the Milwaukee River during storm events. Lotic ecosystems are highly variable (e.g., seasonal changes, storm events), and most research assessing anthropogenic particle pollution extrapolates stream particle loads based on measurements during only one point in time. This limits the accuracy of anthropogenic particle and microplastic dynamics models in rivers, where frequent changes in discharge drives retention and transport of fine particles. In this study, we used automated samplers to collect water from the Milwaukee River during four distinct storm events in the spring and summer of 2018. We quantified anthropogenic particle abundance using standard methods and used nearby USGS gauges to determine discharge. Anthropogenic particle concentrations varied significantly among the four sampling periods, highlighting the temporal variability of anthropogenic particle transport across dates. When data from the sampling periods were pooled, there was a decrease in anthropogenic particle concentration in the water column after storm events, indicating that floods may "flush" microplastics from the river. Unexpectedly, anthropogenic particle concentrations were not correlated with other water quality metrics, including concentrations of total suspended solids, fecal coliform, chloride, nitrate, and sulfate, indicating that these metrics cannot be used to estimate microplastic concentrations and likely have distinct driving factors. These results provide novel insights into the environmental factors controlling anthropogenic particle and microplastic fate and are critical to understanding the role of lotic retention and transport in global plastic budgets.

Does Manipulating Source Gender Predict A Person's Open-Mindedness As A Function Of Sexism?, Tanyelle Annette Galman

Master's Theses

The present research examines whether Communication Source Gender influences a message recipients' level of Open-Minded Cognition, and whether Ambivalent Sexism moderates this effect. Participants were asked to think of themselves as part of a military panel which considers proposals to military spending. Then after reading a proposal from either Cassandra Smith or David Smith, participants were asked to indicate whether they would be open to hearing more from the author. Participants were then instructed to complete the Ambivalent Sexism Inventory (Glick & Fiske, 1996). In total there were 395 participants in this study. Results show that there was no main …

Pseudomonas Aeruginosa Prophages And Engineered Temperate Phages, Genevieve Johnson

Master's Theses

The rising rate of antibiotic-resistant bacteria is a global health concern, and the Pseudomonas aeruginosa is estimated to be a leader in serious threats. Bacteriophages, or viruses that infect bacteria, have gained renewed interest in Western Medicine as an alternative to antibiotics. Phage therapy of P. aeruginosa infections has primarily focused on obligately lytic phages. However, prophages, or phages in the lysogenic life cycle, are estimated to far outnumber obligately lytic phages. In theory, these prophages can be genetically modified to be obligately lytic and thus ideal candidates for phage therapy. To date there has not been a comprehensive analysis …

Ethnic Racial Socialization, Ethnic Identity, And Internalizing Outcomes In The Context Of Stress Among Mexican Origin Families, Sarah Alicia Jolie

Master's Theses

Research demonstrates that it is important to study the impact of cultural factors on child development and well-being (Garcia Coll et al., 1996). Among Latino youth, ethnic racial socialization and ethnic identity have been associated with positive outcomes, such as academic competence and lower reports of internalizing problems (Liu & Lau, 2013; Berkel et al., 2010; Umaña-Taylor & Guimond, 2010). Additionally, there is evidence of ethnic identity mediating the association between ethnic racial socialization and child internalizing symptoms among Mexican-origin adolescents (Umaña-Taylor et al., 2014). However, more research is needed in examining these associations within stressful contexts relevant to Latino …

Rethinking Intimacy: Liberation Through Decolonial & Queer World-Making, Michelle Mae Mcguire

Master's Theses

Relationships play an important role in both the private and public spheres of our lives. If we understand our bodies to be the vessels through which we interact with all other objects, we come to understand the process of world-making as a summation of our relationships. Intimacy is the prevailing structure that helps assign meaning to these relationships. Intimacy binds together unfixed spatial and social relations that stretch across time and space. This essay examines the three intersecting sets of relations involved in intimacy as a means to deconstruct heteropatriarchal order and highlight the multiplicity of attachments and relationships that …