Digital Commons Network^™

Effects Of Mature Recombinant Gdf9 And Bmp15 On In-Vitro Maturation Of Bovine Oocytes And Subsequent Embryo Development And Effects Of Antioxidants On Blastocyst Re-Expansion Rates, Jamie Thompson

Master's Theses

In-vitro produced embryos (IVP) differ greatly from in-vivo derived embryos (IVD) in gene expression, metabolism, development, and cryotolerance which limit the widespread use of this technology. In-vitro maturation (IVM) is one of the most important components for successful in-vitro embryo development. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) have been found to be essential during oocyte maturation and thus female fertility. These proteins are oocyte secreted factors (OSFs) and are produced with pro- and mature protein regions where the pro-regions are thought to aid in protein folding and dimerization where heterodimerization the two proteins has been …

Integrative Analyses Of Genomic And Metabolomic Data Reveal Molecular Mechanisms Associated With Uterine Disease Traits In Transitioning Dairy Cattle, Leanna C. Sanchez, Mohammed Abo-Ismail, Daniel Peterson, Fernando Campos-Chillon

Master's Theses

The metritis complex (MC), characterizing post-partum uterine diseases in dairy cattle has negative implications on animal welfare, production efficiency, and the economic stability of the dairy industry. The studies in this thesis aimed to investigate the genetic architecture of the metritis complex and identify genomic regions and metabolites associated with the development of MC. Thereby enhancing our understanding of the biological pathways and molecular mechanisms involved in the pathophysiology of MC during the transition period in Jersey and Holstein dairy cattle.

Chapter 2 sheds light on the previous work done on MC. The goals of this review were to (1) …

Southern Sea Otter (Enhydra Lutris Nereis) Fur Morphology, Thermal Function, And Buoyancy Across Ontogeny, Kate Riordan

Master's Theses

Sea otters (Enhydra lutris) have the densest fur of any animal, and the hairs function to trap a layer of air that is used for insulation. When a sea otter is born, it has a natal pelage (baby fur), and sea otters eventually molt that fur and replace it with a pelt resembling the adult fur. Sea otter fur is composed of 3 types of hairs: underhairs, intermediate hairs, and guard hairs. Sea otters are more susceptible to the negative effects of oiling from oil spills compared to other marine mammal species because they solely rely on fur …

Composite Solid Rocket Motor Case Optimization With Matlab Genetic Algorithm, Aleksandr Breit

Master's Theses

This study utilizes MATLAB genetic algorithm (GA) to perform optimization on a solid rocket motor (SRM) chamber structure. The (SRM) structure is modelled as a cylindrical composite laminate where unidirectional fiber orientations are the primary design variable in the optimization problem formulation. Additional design variables such as chamber length, diameter, and internal pressure are altered along with the ply angles to minimize the safety margin towards a near-zero value and encourage efficient layup design. Classical lamination theory with Tsai-Wu failure criterion are used to calculate ply-level safety margins. A thickness minimization scheme is employed to reduce the ply count from …

Hydric Physiology Of Lizards, Savannah Weaver

Master's Theses

Chapter 1: Animals can respond to extreme climate by behaviorally avoiding it, or by physiologically coping with it. We understand behavioral thermoregulation and physiological thermal tolerances, but water balance has largely been neglected. Climate change includes both global warming and changes in precipitation regimes, so improving our understanding of organismal water balance is increasingly urgent. We assessed the hydric physiology of endangered Blunt-nosed Leopard Lizards (Gambelia sila) by measuring cutaneous evaporative water loss (CEWL), plasma osmolality, body mass, and body condition throughout their active season. On average, G. sila had low CEWL that is likely desert-adaptive, and high …

Effects Of Intertidal Position On The Response To Oxygen And Desiccation Stress In The Common Acorn Barnacle, Balanus Glandula, Megan M. Dotterweich

Master's Theses

Sessile invertebrates in the rocky intertidal experience intermittent periods of air exposure due to tidal flux, presenting risks of temperature extremes, hypoxia, nutrient limitation, and most dangerously, desiccation. Microscale variation in severity and frequency of these risks is widely dependent on vertical position within the intertidal zone. Common acorn barnacles (Balanus glandula) have a wide vertical distribution in the intertidal, creating large differences in microhabitat between the highest and lowest individuals in the population. This study set out to explore whether tidal position dependent differences exist in the response to oxygen and desiccation stress in B. glandula. …

Development Of Wiskott-Aldrich Syndrome Knock Out Protocol For Drug Substance Assay Development, Julia C. Hanna

Master's Theses

Wiskott-Aldrich Syndrome (WAS) is a rare X-linked primary immunodeficiency affecting approximately 1 in 100,000 live XY births in North America and is caused by a mutation to the WAS gene which is expressed across hematopoietic lineages. The WAS protein (WASp) plays a role in regulating actin polymerization. On a cellular level, there are a variety of effects of a lack of WASp or expression of a dysfunctional WASp protein for patients including issues with migration, adhesion, chemotactic response, phagocytosis, activation, and proliferation across different cell types in addition to reduced platelet size and output. This can lead to several systematic …

Constraint-Driven Open-World Scene Generation, Hunter Borlik

Master's Theses

We introduce an alternative method for open-world scene generation. In this thesis, Graph-based Wave Function Collapse (GWFC) is integrated with Space Colonization Algorithm (SCA) and used to place objects in an unstructured 3D environment. This combined algorithm, Space Colonization Graph-based Wave Function Collapse (SC-GWFC), leverages the constraint-based capabilities of GWFC and the ability of SCA to populate arbitrary 3D volumes. We demonstrate that objects of variable scale can be successfully used with SC-GWFC. Since this algorithm is run in an interactive environment, we demonstrate iterative modifications to a partially complete scene and incorporate PCG into a scene editing process. As …

​ Soilborne Pathogens Of Strawberry In The Central Coast Region Of California: ​ Survey And Cover Cropping With Wheat For Management Of Macrophomina Phaseolina, Mary Steele

Master's Theses

Surveys of the four major soilborne pathogens of strawberry (Fusarium oxysporum f. sp. fragariae, Macrophomina phaseolina, Phytophthora spp., and Verticillium dahliae) to determine their relative prevalence were conducted in Watsonville-Salinas, CA in 2021 and in Santa Maria, CA in 2022. All four major pathogens were detected at relatively similar prevalence in Watsonville-Salinas, between 22% and 31% of sampled fields. In Santa Maria, M. phaseolina was far more prevalent at 52% of sampled fields, the other three falling between 14% and 17%. Additionally replicated greenhouse and field trials were conducted to evaluate the effects of wheat as …

Identification Of Spring And Fall Spawned Eggs And The Genetic Distinction Of Gulf Sturgeon In The Choctawhatchee River, Alabama-Florida, Nicholas Lamey

Master's Theses

The Gulf Sturgeon (GS; Acipenser oxyrinchus desotoi) is a threatened subspecies of Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus) which inhabits the Gulf Coast of the United States. Prior studies of Eurasian sturgeon species and Atlantic Sturgeon have revealed the existence of a dual spawning strategy (i.e. spring and fall). The presence of a fall spawn has also been proposed for GS, but only with the support of circumstantial evidence. This study used molecular techniques to investigate seasonality of GS spawning and the possibility that this has produced unique genetic groups in the Choctawhatchee River. Mitochondrial DNA from suspected …

A Minor Place: A Short Story Collection, Nathaniel Pyle

Master's Theses

In this collection, characters often face interior struggles such as past lives, abusive upbringings, dead partners, and relationship struggles. A Minor Place concerns loneliness and existentialism set with a melancholy, southern gothic setting. Characters toil with their psychological state within a shared and collective universe of the unknown. The characters found within A Minor Place deal with sensitive issues such as loss, isolation, and overcoming their difficulties. A Minor Place implements narratives to showcase a "minor” tonality and coloration and style to accompany this titular minor place.

'Formation In Distinctive Dimensions Of Our Way Of Proceeding': Preparation Of Lay Directors Of Works In Jesuit Schools, Perry T. Petrich Sj

Master's Theses

The purpose of the study was to determine how lay directors of works in Jesuits West secondary schools can be formed to best serve as faith leaders. The study consisted of secondary research in which interview data and surveys from studies on lay formation in Jesuit universities and lay leadership of Bay Area Catholic High Schools were used to determine whether or not formation of lay directors of works in faith leadership is possible, what the objectives of that training should be, and how that training can be offered. This analysis determined that lay formation is both possible and necessary. …

Mapping Effectiveness: Farm Mechanization Initiatives By Lumbini Province Of Nepal (Fiscal Year 2018/19), Ananda Phuyal

Master's Theses

An effective farm mechanization program should increase crop production and be profitable to the benefitting households. Increasing labor shortages and decreasing animal draft power in agriculture demanded farm mechanization to increase food productivity in Nepal. To increase crop yield, the Ministry of Land Management, Agriculture and Cooperative (MOLMAC) of Lumbini Province in Nepal distributed 1994 subsidized tools and machinery in Fiscal Year 2018. The farm mechanization program by the government was an ambitious program and was implemented without research to address the actual need of the geography. However, the program had a positive impact on the community. It is found …

Unaccompanied Migrant Children And Adolescents’ Rights In Colombia: Between Invisibility And Incapacity, Linda María Urueña Mariño

Master's Theses

This thesis centers on an analysis of the situation of unaccompanied migrant children and adolescents transiting through and settling in Colombia, from a children’s rights perspective. These children are being invisibilized, which is causing their unprotection and multiple violations of their rights. Through a critical policy analysis, with a review of Colombian migration and child protection policies, and interviews with governmental and nongovernmental representatives working with migrant children in the country, the study aimed at contrasting policies with the real practices of migrant children protection.

The analysis recurred to Bauman, Butler, Crenshaw, among other authors to show how the Colombian …

Positive Out Of The Negative: Tracking Renewable Energy Projects In Central America, Jordan Ethan Castillo (Miner)

Master's Theses

Central America is undertaking a vast Renewable Energy (RE) transition, due to widespread investments across the region in an array of technologies. These technologies include Community Solar, Wind, and Hydroelectricity. Hydroelectricity has long been the backbone of many countries’ energy grids in the region due to the region’s long history with hydroelectricity. Ambitious climate goals coupled with diminishing hydroelectric power generation opportunities have led to an expansion of investment in Community Solar and Wind energy. The embrace of Solar and Wind has been accelerated due to declining costs for these technologies as they mature. Central America as a case study …

Charity Begins At Home: Investigating How Culture Shapes In-Group Bias In Altruistic Behavior, Angira Shukla

Master's Theses

The paper builds a structural model of altruistic transfers based on identity matches with another individual and estimates the weights that people place on the characteristic matches. We also take a comparative look at the role of culture in these transfers. The culture here is trying to contrast a traditional society versus a cosmopolitan one. I run an experiment in India to examine these differences. I find that in both the urban and rural samples individuals tend to give more to girls and donate largely on the basis of the need of the receiver. I also find that the rural …

Ties That Bind: Allomaternal Care And Cooperation Among Matrilocal And Patrilocal Northeast Indian Tribes, Shreeja Guha

Master's Theses

Why do humans cooperate? Given the extensive range of literature from various disciplines on the puzzle of human cooperation, there is no one answer to that question. In this paper, I look at whether childcare provided by individuals other than the genetic mother, i.e. allomaternal care, can be one possible explanation for the evolution of human cooperation, especially in small communities with no formal childcare. The data for this study comes from field experiments & surveys conducted with a sample of 416 mothers and fathers from matrilocal and patrilocal tribes in North-east India. I present evidence that allomaternal care plays …

From Instability To Civil Liberties: Nonviolent Resistance In Afghanistan, Rachel L. Knowles

Master's Theses

When people have a fundamental issue at stake, giving in is not an option. For these types of conflict, when people’s rights are being violated, when their countries are occupied, or when they are oppressed and humiliated, they need a powerful way to persist and fight back. Oftentimes when people are left with no choice they will use the terrible and destructive nature of violence. For decades nonviolent resistance (NVR) movements have been associated with Gandhi and Martin Luther King, but people have been using nonviolent action for years. In fact, NVR has been a part of political life for …

Freckled Darter (Percina Lenticula) And Other Fishes’ Usage Of Wood Pile Habitat, Noah Daun

Master's Theses

Effectively sampling habitat with the proper techniques is essential for our understanding of the animals that use the habitat, both for individual species and entire species assemblages. Percina lenticula is a species of special concern in the state of Mississippi due to low numbers of historical records. This species is known to inhabit wood pile habitat in rivers, a habitat that has been under-sampled historically due to inefficiency with most commonly used gear. Seines are typically not effective at sampling wood piles, resulting in sampling bias underestimating the number of P. lenticula and potentially other species utilizing such habitats. This …

Comparative Analysis Of Rhizosphere And Endosphere Microbiome Of Different Blueberry Species (Vaccinium Sp.), Niladri Bhowmik

Master's Theses

Blueberries are an important agricultural commodity in all over the United States. Due to its health benefits, there is a huge demand globally, thus expanding the industry. Breeding programs are essential to maintain such industries. Challenges that play a role in contemporary breeding programs are various biotic and abiotic stress factors. Studies have shown that microorganisms are recruited by plants to alleviate them during stressful conditions. Though blueberries have been cultivated for about 100 years, how the microbiome has been affected due to this is poorly understood. We hypothesized that interspecific crosses and artificial selection have significantly changed the microbiome …

Ramie (Boehmeria Nivea): An Underutilized Plant For A Multipurpose Industrial Application; A Perennial Plant With Protein Identification Using Maldi-Ms, A Biosorbent For Hg (Ii) Removal From Water And As A Bast Fiber Production Via Enzymatic Degumming, Abdul Rashid Issifu

Master's Theses

Identifying plant species that can offer life support to humans and animals without damaging the environment is quite challenging. Ramie, one of the oldest fiber crops was studied due to its nutritional value as fodder and other multipurpose applications in, e.g., medicinal, environmental, and textile industrial fields.

With a brief discussion of background in Chapter I, Chapter II focused on using a proteomic method to investigate unknown proteins in the leaves of ramie and to study their benefits to the biological system. PPiase and RuBisCO with molecular weight 15 and 52 KDa were identified as the proteins after peptide mass …

New Insights Into The Paleovegetation Of The Ancient Underwater Forest Located In The Northern Gulf Of Mexico, Karla Lizarraga Garcia

Master's Theses

A well-preserved remnant of an ancient bald cypress forest (hereafter, Underwater Forest) constitutes the only known archive of a glacial refuge on the once exposed continental shelf of the Northern Gulf of Mexico. This forest existed around 41-72 yBP (MIS 3-5) according to the existing dating methods applied on previous studies. As the climate conditions and sea levels changed, the vegetation of this glacial refuge shifted from bald cypress forests to open marshes, altering the vegetation dynamics. The present research analyzed four new cores (21DF-5A, 21DF-5B, 15DF-6, and 16DF-3A), which provided additional insights into the vegetation that once existed in …

Implementation Of Methods For Cell Therapy Research And Assessment Of The Impact Of Cryopreservation, Samantha Lynes

Master's Theses

Cell therapy is the administration of living cells into a patient to prevent or treat a variety of diseases and illnesses. The cell therapy industry is rapidly expanding, and continued research is necessary for manufacturing safe and effective therapies. Although cell therapy manufacturing generally involves cryopreservation processes for storage, there are limited standards for cryopreservation processes and assays required to evaluate cell therapies post-thaw, and limited understanding exists about how a recovery period post-thaw could impact cell health. The overall goal of this thesis was to evaluate the effect of cryopreservation and potential subsequent recovery time on cell viability.

Because …

I’Ll Be Goldenrod And You’Ll Be Aster: The Case For Revolutionizing Western Methods Of Teaching Using Indigenous Ontologies, Joanna Logerfo

Master's Theses

An interesting facet of living as a human in the 21st century is contending with the end of the world. It’s been imagined in a thousand ways over the past twenty years. Will it be zombies? Aliens? An AI revolution? Or will it perhaps be something more mundane, more “down-to-Earth”? The floods, the droughts, the famines, and all the rest of the cataclysmic global events that occur every year have taken center stage in the world-ending debate, parading under a name as threatening and expansive as the Boogeyman: climate change. A recent article from NPR covered the United Nations’ 2022 …

Nutritional Adequacy Of Dietary Intake Among College Students Who Follow A Veget, Kayanna L. Pulliam

Master's Theses

In recent years, forms of the vegetarian diet have been popularized for various reasons, including health benefits, support of animal rights, and greater sustainability claims. The purpose of this cross-sectional study was to examine the diet quality and adequacy and iron status of college students’ diets, and compare eating disorder (ED) risk between self-proclaimed vegetarians and non-vegetarians. The study sample was a convenience sample of students at Louisiana Tech University who were 18 years of age or older (N = 179). Students were recruited via email, flyers, and classroom announcements. Participants completed an online questionnaire with multiple validated tests; The …

Pollinator Diversity On A Shortleaf Pine-Oak-Hickory Restoration Site, Taygan Kohlman

Master's Theses

Land use and cover are increasingly changing due to climate change and anthropogenic activities with many of these changes negatively impacting biodiversity. However, ecosystem restoration may help reverse these patterns. Northern Louisiana was historically dominated by shortleaf pine-oak-hickory forests but has been converted largely to loblolly pine forests with little-to-no herbaceous ground cover. The objective of this study is to determine how different practices on a shortleaf pine-oak-hickory forest restoration site affect pollinator diversity. I sampled pollinators from locations within areas under three different management regimes: 1) prescribed burning with active herbicide treatments for non-desirable plant species, 2) burning only, …

Nutritional Adequacy Of Dietary Intake Among College Students Who Follow A Vegetarian Diet As Compared To Non-Vegetarian Students, Kayanna L. Pulliman

Master's Theses

No abstract provided.

Incorporating Active Learning Into Breastfeeding Education And Lactation Management Training Of The Future Registered Dietitian Nutritionist, Madelyn Sesser

Master's Theses

Background: Breastfeeding has been shown to have multiple benefits for mother and child, yet rates of breastfeeding continue to stay insignificant. Infant nutrition is the base of future health of an individual, yet Registered Dietitians are not provided in depth breastfeeding education during their education.

Objective: The purpose of this study is to evaluate the active learning component of breastfeeding and lactation education modules meant to increase self-efficacy and confidence in the ability to perform lactation education and to obtain feedback regarding the usefulness of the developed education modules in graduate-level dietetic interns.

Design: This study was part of a …

The Role Of Notch3 In Human Adipose Stem Cell Fate, Sydney Mashaw

Master's Theses

Stem cell-based therapies are the future of medicine, as their natural abilities of self-renewal and differentiation have the potential to treat currently incurable diseases. Stem cells are categorized based on differentiation potential, with multipotent stem cells being the most differentiated while still having the ability to divide down defined lineages. Adipose stem cells (hASCs) are easily derived multipotent, mesenchymal stem cells with already demonstrated therapeutic potential. Despite current success, there are a multitude of factors that regulate the physiology and role of adipose stem cells, including transduction pathways and transcription factors, that remain to be researched and fully understood. One …

Effects Of Triptorelin On Global Deoxyribonucleic Acid Methylation In Skeletal Muscle In Male Adolescent Rats, Max Boland

Master's Theses

The purpose of this study was to investigate how treatment with triptorelin, a gonadotropin releasing hormone agonist, to block puberty in adolescent male rats affects their epigenetic regulation via global DNA methylation changes in skeletal muscle and to determine if physical activity has an effect on DNA methylation status during treatment. Twenty-four male, Sprague-Dewey rats were randomly assigned to different groups: 12 treated with 1 μg of triptorelin daily (PB group) while the other 12 received an equivalent volume of saline as a placebo (control, CON) for four weeks. In order to determine the interaction with physical activity, six PB …