Digital Commons Network^™

Assessing The Impacts Of Grazing By Piney Woods Cattle And Controlled Burning On The Herbaceous Layer In An East Gulf Coastal Plain Longleaf Pine Woodland, William Mcfarland

Master's Theses

Fire and grazing are two disturbances which influence vegetation composition and diversity in many ecosystems. In the southeastern United States, longleaf pine plant communities are adapted to frequent fires and are likely to have been grazed by bison in the past. Though bison have been extirpated from this region, Pineywoods Cattle, which were introduced by the Spanish in the 16^th Century, have been fulfilling a similar role in many of these communities. This study examines the effects that combined grazing and controlled burning have on plant diversity, biomass, and community similarity in a longleaf pine savanna in the East …

Youtube Influencers In Bangladesh: Analyzing Message Strategies Of Sponsored Ads In Vlogs, Monira Begum

Master's Theses

Social media influencers, particularly YouTube influencers marketing emerged as a growing industry in Bangladesh. However, research on this topic is limited. This study effectively addresses this gap and following quantitative methods and content analysis, this study mainly examines Influencers and brand categories, the narration of sponsored brands in advertising, influencers’ interactivities, and engagement with the followers, and message strategies in ads employing Taylor’s six-segment message strategy wheel, advertising appeals, and message formats. Results indicate that influencers' dominant vlog content is entertainment, as well as technology as prevalent brand characteristics in the study.

Regarding the narration of the sponsored ads, this …

Understanding The Variability Of Circulation And Water Quality At Experimental Oyster Leases In The Mississippi Sound Using A High-Resolution Nested Modeling Approach, Shihab Hossain Saran

Master's Theses

Four 50-acre experimental oyster leases; two near Saint Louis Bay (StLB) and two near Pascagoula Bay (PB) were recommended to provide science-based guidance for oyster reef restoration efforts in response to continued limited productivity of oysters in the Mississippi Sound (MSS). Two-way nested simulations using ROMS within COAWST modeling framework have been run with improved forcings to analyze circulation, salinity and temperature variability within and around the lease areas under the extreme freshwater inflow from Bonnet Carré Spillway (BCS) openings and local rivers in 2019. High-resolution atmospheric forcing data is crucial because diurnal sea-land breeze affects salinity variability by increasing …

Peripheral Vascular Function In Young Adults With Increased Risk Of Cardiometabolic Disease, Ryan S. Aultman

Master's Theses

Objective: To determine if young adults with elevated metabolic syndrome severity scores (MetS_index) suffer from peripheral vasculature dysfunction.

Methods: Mean arterial pressure (MAP), Femoral (FBF) and Brachial Blood flow (BBF), Femoral (FVC) and Brachial Vascular Conductance (BVC), and Tissue Saturation Index (TSI) were assessed in twenty-four age (19 + 2.25 years), Sex (male: n=18; female: n=6) and Race (White: n=4; Black/African American: n=4; Asian: n=16) matched individuals during post-occlusive reactive hyperemia (RHBF), passive limb movement (PLM) and Functional Sympatholysis (FS) trials. Carotid femoral pulse wave velocity (cfPWV) was also collected at baseline, and all values were compared between …

Effects Of Extreme Starvation On The Fasting-Adapted Western Rattlesnake (Crotalus Oreganus), Brandon R. Kong

Master's Theses

Ambush-hunting snakes have long been of great interest in the biology of fasting and starvation, as they are adapted to withstand well over a year without taking a meal. Well-justified ethical constraints have limited our understanding of their biology at the limits of these abilities. Unfortunate circumstances, in which the California Department of Fish and Wildlife confiscated over 50 Western Rattlesnakes (Crotalus oreganus) from an animal hoarder that had long neglected them, culminated in the euthanized animals ending up in the hands of our lab. Some of these snakes were extremely starved, being only one-half to one-third of …

Effect Of Enzymatic Pretreatment On Biomethane Production From Olive Pomace, Ningjing Zhong

Master's Theses

In 2023, approximately 2.36 million metric tons of olive oil were produced globally. Olive pomace, a byproduct of the flesh and pits left after olive oil extraction, presents environmental challenges when used as landfill due to its high polyphenol and organic contents, or when combusted due to greenhouse gas emissions. Its potential as animal feed is limited, yet it holds promise for methane production via anaerobic digestion (AD), providing a source of renewable energy. However, the highly crystallized polysaccharides in olive pomace, such as cellulose, hemicellulose, and pectin, impede its conversion to methane, and the high polyphenol content inhibits methanogen …

Vaccines At The Intersection Of Religion And Politics, Charles Downey

Master's Theses

Vaccine hesitancy – a delay in acceptance or refusal of vaccines despite their availability – is a rising threat to global health and has increased in recent years in higher income countries. This is a complex phenomenon with the potential to undo decades of progress toward eradicating communicable diseases. The recent COVID-19 pandemic introduced a new wave of vaccine hesitancy, with evangelical Christians as leading actors in the movement away from vaccines in the United States. The present study investigated factors related to vaccine hesitancy and sources of health-related information among members of evangelical Christian churches in and around Hattiesburg, …

Identifying New Potential Breeding Pond Sites For The Dusky Gopher Frog In Southern Mississippi, Shelbie Kellum

Master's Theses

The main objective of this thesis was to identify potential new pond sites across southern Mississippi for the critically endangered dusky gopher frog (DGF). Ephemeral ponds are the preferred breeding habitat for the species, but they are quite rare in the region due to the diminishment of the longleaf pine ecosystem. This research addresses gaps in the current literature by providing a broad overview of dusky gopher frog breeding habitat identification. LiDAR derived datasets as well as tools within ArcGIS Pro were utilized in order to perform spatial analysis on sites of interest in south Mississippi. By using this methodology …

Development Of A Flow Cytometry Method For The Identification And Enumeration Of Enterococcus Faecalis, Riza Magbitang

Master's Theses

A multi-color flow cytometry method coupled with fluorescent staining was developed to detect and quantify E. faecalis concentrations in coastal waters as an alternative to the traditional membrane filtration method. The developed method was used to characterize E. faecalis during batch growth and yielded consistent results across the various physiological states of the cells. Additionally, the application of propidium iodide (i.e., a viability stain), afforded the method the ability to differentiate between cells with intact and compromised cell membranes, which raises the potential of quantifying cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked …

On An Unshakeable Foundation: An Archaeological Investigation Of The Postemancipation Black Community Of Bass Street, The Church They Built, And The Lasting Identity They Formed In Nashville, Tennessee, Clélie Elizabeth Cottle Peacock

Master's Theses

The Bass Street Community lived along the northern base of St. Cloud Hill in Nashville, just below the Civil War-era Union fortification, Fort Negley. The fort was built and defended by conscripted free, enslaved, and self-emancipated Black/African Americans and soldiers from the U.S. Colored Troops; some of whom stayed, built residences, founded a church, and established a shared community identity. The objects they left behind reflect a time of transition in the postemancipation urban South.

The former Bass Street Community enclave is a subset of the Fort Negley archaeological site (40DV189). My thesis examines artifacts from the Bass Street Baptist …

Remembering, Forgetting, And Artifact Deposition In Late Prehistoric Florida, Andrew Bowen

Master's Theses

Material culture plays a fundamental role in memory processes. Artifacts are an important means of both connecting with and cutting ties with a real or imagined past. They can be reminders or symbols of connection to other people, places, things, powers, or memories, making them key components within networks and relations. The way materials are deposited or destroyed often follows specific customs or responds to environmental factors. Specific situations, like interactions with outside groups or socio-political changes, prompt particular depositional events. During the Mississippi period, such circumstances were prevalent throughout the Southeast. By treating artifacts in specific ways during deposition, …

Investigating The Response Of The Eastern Oyster (Crassostrea Virginica) To Low Dissolved Oxygen Conditions, Abiola Obafemi

Master's Theses

The Eastern oyster (Crassostrea virginica) requires dissolved oxygen (DO) of about 4 mg/L to support proper physiological function. Hypoxia and/or microxia occur when DO decreases to ≤ 2 mg/L. Salinity and temperature changes resulting from large-scale freshwater influx have been identified as key factors influencing the oyster populations in the Mississippi Sound. It is important to investigate the impacts of DO conditions on oyster mortality, biomass, size, and condition during these freshening events. Under controlled laboratory experiments, this thesis investigated 1) the response of oysters to microxic, hypoxic, and normoxic conditions 2) the response of oysters to combined hypoxia and …

Antimicrobials Produced By Ants: Chemical Properties And Microbe Specificity, Katy Chon

Master's Theses

Antibiotic resistance is a growing public health concern worldwide, and for over a decade, we have been witnessing the growth of difficult to treat infectious diseases caused by bacteria that are becoming resistant to antibiotics. This resistance can be mostly related to the misusing and improper use of antibiotics in both humans and animals. To restore this problem, humans have turned to new sources for the production of antibiotics. In this study, we focused on using ants. Social insects, including ants and bees, have faced strong disease pressures during their evolution and have developed a range of methods to fight …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Spatial And Abiotic Effects Of Urbanization On Small Mammal Communities, Bri Casement

Master's Theses

Urbanization, the phenomenon of increasing human population density and artificial structures, is known to decrease the richness, abundance, and diversity of native wildlife populations, fragment natural areas, and increase human and human-commensal animal interactions with wildlife. Dynamics of species persistence in habitat fragments is often understood in the framework of island biogeography theory (IBT), with habitat fragments being analogous to oceanic islands. Despite the conceptual similarities between oceanic islands in IBT and urbanized habitat fragments (fragment size, fragment age, and distance to immigration sources), there are important differences. In urban fragments, the matrix is not completely impassible, habitats within fragments …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Neurogenomics Of Competition And Parental Care In A Socially Polyandrous Shorebird, Tessa Mathur Patton

Master's Theses

Despite sharing largely similar genomes, females and males of the same species exhibit morphological, physiological, and behavioral differences. Sex-role reversal stems from a unique case of sexual selection in which females compete for mates and males conduct the majority of parental duties. We examined the extent to which aggression and parenting may be mediated by different neurogenomic mechanisms by comparing females versus males in the northern jacana (Jacana spinosa), a sex-role reversed shorebird. We hypothesized that sex differences in neural transcriptomic profiles explain sexual dimorphism in behavior. We conducted RNA-Seq in two brain regions within the social behavior network: the …

Assessment Of Georgia’S Royal Catchfly (Silene Regia) Along A Forest-Edge Gradient In A Remnant Prairie Community, Megan Brooks

Master's Theses

Royal catchfly (Silene regia) is a perennial prairie forb known to grow in rocky, alkaline soils within tallgrass prairies of the eastern United States. S. regia is recorded from twelve states, including Georgia, and is now confined to fragmented habitats due to the loss of 95% of its natural environment. The species is now listed as critically imperiled in the state of Georgia. Attempts at reintroduction via outplanting have only shown limited success, necessitating more research on the environmental conditions in which this plant survives in its southern-most distribution. This study investigated the local ecological factors influencing the …

Fleeing Towards Healing: How Cam Can Heal Refugee Communities, Sarah B. Shaalan

Master's Theses

Alternative methods of healing can be found across the globe and are used by all kinds of people including refugees. This thesis will explore the potential benefits of Complementary and Alternative Medicine otherwise known as “CAM” as a supplementary strategy for helping refugees to deal with stress, Post Traumatic Stress Disorder (PTSD) and other harmful effects sustained on their journey and lives as displaced peoples. I will do so by first situating my project in the literature on Eurocentrism in healing practices and the emergence of CAM as an alternative. Then, using extensive primary and secondary sources, supplemented by an …

Neuroinflammation And Gut Barrier Function Post Traumatic Brain Injury, Anne Sullivan

Master's Theses

Traumatic Brain Injury (TBI) is a major cause of morbidity and mortality that afflicts millions of people in the United States annually. TBI mice had significantly increased expression of the pro-inflammatory cytokines and decreased expression of tight junction proteins CLDN5, CLDN12, and OCLD in brain lesion tissue. TBI mice exhibited a significant accumulation of FITC in the middle and distal part of the small intestine compared to sham mice. TBI mice exhibited an increase in pathogenic bacteria Enterobacteriaceae and a decrease in beneficial bacteria lactobacillus. TBI mice exhibited a trend toward an increase in permeability compared to no treatment. These …

The Role Of Micrornas In Lung Inflammation After Alcohol And Burn Injury, Connor O'Neal Guzior

Master's Theses

Acute lung injury (ALI) and the subsequent development of acute respiratory distress syndrome (ARDS) are among the leading causes of post-burn morbidity and mortality. MiRNAs are small non-coding RNA molecules that are known to regulate gene expression and help regulate lung homeostasis. In this study, we profiled four anti-inflammatory miRNAs (miR-146a, miR-150, miR-194, and miR-671) in lung tissue following an alcohol and burn injury. We found that miR-146a and miR-671 were significantly downregulated in lungs one day after combined injury, leading to increased lung inflammation and pathology. Biological sex did not have an effect on this pathophysiological process. We also …

Engineering An Injectable Hydrogel With Self-Assembling 3d Vasculature, Kendyl Cohn

Master's Theses

This research developed methods for culturing self-assembling capillaries in an injectable gel as a potential method for vascularizing tissue-on-a-chip models to mimic physiological drug delivery. Additionally, a mathematical model was developed as a tool for understanding nutrient delivery and comparison of potential delivery systems. Organs-on-a-chip provide novel platforms for studying biology and physiology in 3D, allow exploration of tissue engineering on a manageable scale, and serve as models for drug screening and drug-delivery testing.

Methods were first developed for co-culture of endothelial cells and fibroblasts (3T3s or HDFs) in 2D, evaluating culture time, seeding density and ratio of HUVECs and …

Design And Implementation Of A Vision-Based Deep-Learning Protocol For Kinematic Feature Extraction With Application To Stroke Rehabilitation, Juan Diego Luna Inga

Master's Theses

Stroke is a leading cause of long-term disability, affecting thousands of individuals annually and significantly impairing their mobility, independence, and quality of life. Traditional methods for assessing motor impairments are often costly and invasive, creating substantial barriers to effective rehabilitation. This thesis explores the use of DeepLabCut (DLC), a deep-learning-based pose estimation tool, to extract clinically meaningful kinematic features from video data of stroke survivors with upper-extremity (UE) impairments.

To conduct this investigation, a specialized protocol was developed to tailor DLC for analyzing movements characteristic of UE impairments in stroke survivors. This protocol was validated through comparative analysis using peak …

Assessment Of Caloric And Micronutrient Intakes In Female Collegiate Student Athletes, Rachel L. Victor

Master's Theses

Nutrition can play a key role in athletic performance. Optimal nutrition can help athletes maintain their overall health and well-being and can contribute to training adaptations, recovery, performance, and injury prevention. Many studies focus on the intakes and importance of macronutrients in athletic performance. However, micronutrient intakes may also play important roles. Therefore, the purpose of this study was to assess the calorie and selected micronutrient intakes of female collegiate student-athletes across a variety of sports. The participants were instructed to complete 3-day food records which were analyzed and compared to their individual nutrition needs. Results of this study indicated …

Hydrologic Response Of Meadow Restoration Following The Removal Of Encroached Conifers, Oriana Ramirez

Master's Theses

Meadows are important within forest ecosystems because they provide diverse species habitats, facilitate water cycling, help with sediment capture, aid in carbon sequestration, and create natural fire breaks in forested regions. However, fire suppression, poor grazing practices, and climate change have accelerated the encroachment of conifers into historical meadow habitat. This has led to an extensive loss of meadow habitat within the Sierra Nevada and Cascade Mountain Ranges. Therefore, the purpose of this study is to quantify changes in percent soil moisture and groundwater levels following the removal of encroached lodgepole pine (Pinus contorta) in a historic meadow habitat …

Emerging Diseases, Abiotic Disorders, And Macrophomina Root Rot Management Of California Strawberry, Cooper Calvin

Master's Theses

Strawberry is an economically important crop in California, with an estimated value of $2.68 billion in 2023. In California strawberry production, mitigation of low plant health and yield often focuses on major soilborne pathogens, while the contribution of minor pathogens and abiotic disorders to production shortcomings are often overlooked. The objectives of the first project in this thesis are to determine the pathogenicity of multiple minor pathogens and quantify other biotic and abiotic factors that can reduce plant health such as viruses and soil salinity. Two pathogens of the black root rot complex, Pythium spp. and Rhizoctonia spp. as well …

Red Raspberries Attenuate Blood Glucose Spike Of A High Glycemic Load Breakfast Cereal, Dante J. Martin

Master's Theses

Blood glucose (BG) spikes increase chronic disease risk in those with and without diabetes. The fiber and phytonutrient content of fruit, such as raspberries has the potential to attenuate the BG spike of a high glycemic load (HGL) meal. We examined the effect of red raspberries (RRs) on the BG spike when added to a HGL meal. In this randomized cross-over trial, 16 university students were given breakfast cereal and almond milk (control), the control breakfast with 140g added RRs, and the control breakfast with sugar equivalent to the sugar content of the RRs (50% sugar increase from control). Meals …

Balancing Darkness And Visibility: An Algorithmic Approach To Light Placement In Low-Light, Ray-Traced Scenes, Briana Kuo

Master's Theses

In recent years, digital media has seen incredible advancements in rendering visually stunning computer graphics scenes. Photo-realistic games, animated films, and more leave viewers blown away by the sheer beauty of their graphics. However, challenges arise when depicting dark scenes, often resulting in visual monotony and difficulty in comprehension due to insufficient detail within the scene. In order to enhance readability and visual interest of a scene, additional, artificial lights can be placed throughout a scene to enhance the aesthetic. These lights, however, must be strategically placed in order to retain an essence of darkness and maintain the delicate balance …

The Effect Of Probiotic Supplementation On Serum Metabolite Concentrations In Young Holstein Heifers, Emily G. Meissner

Master's Theses

The objective of this trial was to evaluate the effect of a multi-strain probiotic on serum concentrations of selected metabolites in young calves. Holstein heifers (n = 112) from a commercial dairy were enrolled within 48 h of birth and placed into individual hutches. Calves were weaned at 60 d of age and remained in their hutches until 90 d of age, when they were moved to group housing. Calves were placed into 2 treatment groups: 1) control and 2) B. subtilis, B. lichenformis, L. animalis, and P. freudenreichii probiotic (Bovamine Dairy Plus, Chr. Hansen, Milwaukee, WI). Control calves received …

Deciphering The Contribution Of Microglia To Neurodegeneration In Friedreich's Ataxia, Sydney N. Gillette

Master's Theses

Friedreich's ataxia (FRDA) is the most prevalent inherited ataxia, affecting one in every 50,000 individuals in the United States. This hereditary condition is caused by an abnormal GAA trinucleotide repeat expansion within the first intron of the frataxin gene resulting in decreased levels of the frataxin protein (FXN). Insufficient cellular frataxin levels results in iron accumulation, increased reactive oxygen species production and mitochondrial dysfunction. Tissues most heavily impacted are those most dependent on oxidative phosphorylation as an energy source and include the nervous system and muscle tissue. This is evident in the clinical phenotype which includes muscle weakness, ataxia, neurodegeneration …