Digital Commons Network^™

Drivers Of Microparticles Abundance In Freshwater Fish And Macroinvertibrates, Elizabeth Kazmierczak

Master's Theses

Anthropogenic microparticles (MP) (i.e., particles < 5 mm) are defined as materials of manufactured origin found in the environment. They are widespread and a rapidly emerging global contaminant. MP include microplastics (< 5 mm), as well as other microfibers or particles, which can be synthetic, semi-synthetic and non-synthetic. Rivers are a critical source of plastics to oceans, and MP within rivers are retained in part though biological processes, including ingestion. MP ingestion by freshwater biota varies by proximity to point sources (e.g., wastewater treatment plants; WWTP), overall environmental concentrations, and trophic level and functional feeding groups, but few studies have examined these factors together. In our first study we examined fish collected in the North Shore Channel of the Chicago River to determine MP abundance within organisms. We then compared trophic position (i.e., δ15N) and proximity to a WWTP. With all species combined, MP concentration were not significantly different across study sites, but stable isotopes increased downstream. Considered individually, MP abundance relative to WWTP proximity varied by species, which we attributed to habitat preference and mobility. In a second project, we collected macroinvertebrates from three different watersheds in North America and compared the abundance of MP in macroinvertebrates to concentration within the environment (i.e., water column, surface, and benthic). Functional feeding groups were a significant predictor in the amount of microparticles found in macroinvertebrates. Additionally, the concentration of water column microparticles showed a positive relationship with all macroinvertebrate’s microparticle abundance. Results will aid in understanding the ecological impacts of MPs on aquatic food webs and the pathways in which they can enter.

Sadomasochistic Sensations: A Phenomenological Analysis Of Black Ontology, Embodiment, And Transcendence, Sam Forrey

Master's Theses

Over the past fifteen years, feminists have challenged the notion that sexual sadomasochism perpetuates racial and gender-based violence, and instead claim that the practice can be a powerful site to control and subvert oppression. In this thesis, I contribute to this growing discourse and argue that sensations experienced during practices of Black sexual sadomasochism and attention to the feelings and affects that arise from them can be used to mediate Black being in a violently antiblack United States. Through applications of Black feminist thought, Black anti-humanism, and phenomenology, I claim that antiblack oppression denies Black people the experience of tactile …

Lactobacillus-Mediated Phage Induction And Inhibition Of Growth Of Escherichia Coli, Natalie Stegman

Master's Theses

Bacterial species inhabit both the “healthy” and symptomatic female urinary tract. Lactobacillus species are frequently dominant members of the healthy urinary tract microbiota (urobiome), and thus have been associated with the lack of lower urinary tract symptoms (LUTS). In contrast, colonization by Escherichia coli is the most common cause of urinary tract infections (UTIs). Prior research has found that strains of Lactobacillus can inhibit the growth of uropathogens such as E. coli as well as vaginosis-associated pathogens. Investigations into the means by which lactobacilli inhibit pathogenic growth have primarily focused on their production of hydrogen peroxide (H2O2) and lactic acid, …

The Effect Of Environmental Pollutants On The Spotted Turtle Gut Microbiota, Roza Gawin

Master's Theses

Heavy metals are a common pollutant from many industrial processes. In addition to the various diseases that these pollutants cause, they can also affect the composition and structure of the gut microbiome of many organisms which is in many cases associated with overall organism health. The effect of these pollutants on the gut microbiota of the spotted turtle is unknown. Here we characterize the gut microbiome of the spotted turtle and explore the effect of various environmental pollutants on its composition. We found significant differences in the composition of the gut microbiome based on turtle sampling location (geographic), turtle sampling …

Pattern, Process And The Evolution Of Sexual Dimorphism In Three-Spine Stickleback Fish Gasterosteus Aculeatus From Vancouver Island, British Columbia, Katie Van Dame

Master's Theses

Males and females of a species often differ from one another in phenotype—a phenomenon known as sexual dimorphism. Sexual dimorphism is most often attributed to sexual selection. However, sexual dimorphism also derives from natural selection when resource competition generates disruptive selection favoring specialization on different resources, resulting in niche partitioning and the evolution of dimorphism in traits related to competition. Sexual dimorphism may be an important route to increased individual fitness (i.e., more resources, more survival). For example, ecologically driven divergent selection between sexes may derive from differences in their environments, resulting in the evolution of dimorphism in traits related …

History & Evolution Of Plastic Mulching Technology: An Ethnography Of California Strawberry Plasticulture, Kalea Conrad

Master's Theses

This ethnography draws on key informant interviews and participant observation to produce a case study about plastic mulching as an important industry standard in specialty crop production. It documents the historic adoption and present role of plastic mulching technology in California strawberry production and addresses the characteristics of commercial standard management that inhibit the adoption of alternative mulching practices. It complements extensive research on strawberry pathology and fumigation, breeding innovations, fertilizer and pesticide treatments, and maximization of yields to incorporate participants’ lived experiences and knowledge of mulch origins, the technology adoption process, mulch benefits and drawbacks, the state of agricultural …

Seal Counting On Our Plages (S.C.O.O.P.), Kaanan Kharwa

Master's Theses

The Vertebrate Integrative Physiology (VIP) lab monitors the population of northern elephant seals at the largest mainland breeding colony, located at Piedras Blancas (San Simeon, CA). As the population expands, more human-seal interactions and conflicts over land use occur. The VIP lab's work informs California State Parks and helps with the management of the rookery. Currently, members of the VIP lab fly a drone over the beaches, capture multiple images, and manually count the seals, which takes around 14 to 21 hours of analysis per survey. Machine learning methods such as Convolutional Neural Networks (CNN) and Region-based Convolutional Neural Networks …

Development Of Extruded Puffed Snack Utilizing Carrot Pomace, Bean Flour, And Rice Starch As Primary Ingredients, Julia Zoe

Master's Theses

Every year, the carrot industry worldwide wastes up to 50% of carrots as raw material due to processing (Šeregelj et al., 2020). When carrots are juiced, the remaining fibrous pulp is often discarded as carrot pomace. In order to redirect this food waste, the first objective of this research was to optimize formulations for an extruded puffed snack that is most similar to commercially available puffed snacks, utilizing baby lima bean flour and carrot pomace

Hardness (kg), cross-sectional expansion ratio, bulk density (g/cm³), moisture content (%), water activity, specific mechanical energy (kJ/kg), carotenoid concentration (ppm), and total dietary …

Survey Of Soilborne Pathogens Infecting Strawberry In Oxnard, California & Effect Of Planting Date And Chill Treatment On Yield Of Strawberry, Samantha Z. Simard

Master's Theses

Survey to quantify the frequency of the five main pathogens (Macrophomina phaseolina, Fusarium oxysporum f. sp. fragariae races 1 and 2, Verticillium dahliae, and Phytophthora spp.) infecting strawberry in the Oxnard growing district of California. M. phaseolina was the most prevalent pathogen detected in diseased plants in the Oxnard growing district, present in 67.6% of samples positive for at least one pathogen. F. oxysporum f. sp. fragariae race 1 (35.3%), V. dahlia (8.8%), F. oxysporum f. sp. fragariae race 2 (5.9%) and Phytophthora spp. (2.9%) followed at lower prevalence. Associations were found between fewer drip irrigation lines …

Terrestrial Salinization Impacts On Growth And Behavior Of A Common Detritivore Armadillidium Vulgare (Isopoda), Danielle Harmoney Orcutt

Master's Theses

Climate change and human activities are changing the nutritional landscape by increasing the availability of some essential elements and decreasing others. Sodium (Na) is an essential element in heterotrophs, which is required for water regulation, nerve function, and muscle function. Heterotrophs can acquire Na through diet and environment, often experiencing limited availability of Na due to plants concentrating Na 10-100 times less than heterotrophs need. The Sodium Subsidy-Stress (SSS) hypothesis posits that organismal performance follows a hump-shaped curve of a physiological response, where Na acts as a subsidy, increasing organismal performance such as growth until an optimal threshold is reached, …

The Post-Closet Home: Queer Domesticity, Enclosure, And Queer Southern Gothicism In Lee Mandelo's "Summer Sons", Andrew Dylan Dampier

Master's Theses

In current discussions of queer theory, terms such as “post-gay” leave room for misrepresentation. The premise behind this term serves a primarily cisgenderheterosexual agenda by promising the assimilation of queer individuals into the dominant society. For this reason, I prefer to align the term “post-closet” with the queer person’s journey towards, and past, such societal barriers while maintaining the significance of foundational queer theories. In his novel Summer Sons, Lee Mandelo depicts Andrew Blur’s journey through the grief-ridden confines of the closet as he seeks a new identity following the death of his best friend. Mandelo accomplishes this by juxtaposing …

Oral Nutritional Supplement Prescription And Adherence Perspectives, Kristyn Bordelon Hood

Master's Theses

Oral nutritional supplements (ONS) are nutrient-dense beverages that aid in meeting the nutrient needs of patients. Recent research has indicated that patient adherence to prescribed nutritional supplements may depend on palatability, tolerance, and taste fatigue. Registered Dietitian Nutritionists (RDNs) are healthcare professionals who may prescribe these supplements directly or make recommendations to the patient’s physician for a prescription, thereby influencing patient adherence. Little research has been documented regarding taste fatigue and the influences of the RDN’s decisions on patient adherence to nutritional supplement adherence. The purpose of this study was to gain an understanding of the perspectives of nutrition professionals …

Robotic Odor Source Localization Using Vision And Olfaction Sensing, Sunzid Hassan

Master's Theses

Robotic Odor Source Localization (ROSL) technology allows autonomous agents like robots to find an odor source in unknown environments. A successful odor source location depends crucially on an effective navigation algorithm that directs the robot towards the odor source. This thesis is a combination of three projects. First, we detail development of a versatile multi-modal robotic platform for ROSL real-world ROSL experimentation and discussed real-world validation of a traditional olfactionbased ROSL algorithm. Secondly, we introduced vision in ROSL by proposing a fusion navigation algorithm that integrates deep-learning enabled vision and olfaction-based navigation. This hybrid approach tackles challenges such as turbulent …

Development Of A Urothelial Cell Line Expressing A Caspase-1 Biosensor To Measure Cellular Inflammatory Responses To Potential Uropathogens, Nikita Riya Kesav

Master's Theses

Urinary tract infections are one of the most common bacterial infections, historically implicating Escherichia coli as the causative pathogen. However, it is now known that there are a variety of bacteria that exist within the urinary tract, acting as either beneficial uroprotectives or opportunistic uropathogens. Furthermore, there are significant gaps in our understanding as to how these bacteria interact with the urothelium and what role each plays in maintaining or disrupting homeostasis. Research has shown that Toll-like receptor 4 (TLR4) signaling and Nod-like Receptor Protein 3 play a role in inflammasome activation during infection, triggering caspase-1 activation and inducing release …

A Matter Of Life And Death: The Conceptualization And Theological Significance Of Blood In The Pentateuch, Jared Greer

Master's Theses

This 2024 Master of Theological Studies thesis at Lipscomb University analyzes the conceptualization and theological significance of blood within the Pentateuch. While the thesis does attend to various anthropological concerns (i.e., the sociocultural understandings of blood that inform the text), it is more chiefly concerned with how such understandings of blood contribute to the broader theology of the Pentateuch. Using a canonical interpretive approach that respects both the diachronic nature of the text and the theological unity intended by its final redactors, the thesis primarily explores the two principal contexts in which blood appears in the Pentateuch: Israel’s cultus and …

Testing Strategies To Develop A Mucosal T Cell Inducing Vaccine For Hiv-1, Reynolds Boone

Master's Theses

Despite numerous efforts, HIV vaccine is still not available. Because HIV Envelop protein varies among subtypes and isolates, it is impossible for one vaccine against HIV Env to induce neutralizing antibodies against all subtypes and isolates. Previous vaccine attempts inducing HIV-specific CD4+ T cells have even been shown to enhance the HIV infection because HIV preferentially infects activated CD4+ T cells. It is known that CD8+ cytotoxic T cells (CTLs) play an important role in controlling HIV infections. We hypothesize that a vaccine inducing only CD8+ CTLs but not CD4+ T cells will be effective in preventing HIV infections. Thus, …

The Effects Of Conditional Loss Of Myosin Binding Protein H-Like On Cardiac Function, Hope Victoria Burnham

Master's Theses

Dilated cardiomyopathy (DCM) is a common cause of heart failure with a strong hereditary component. Mutations in the myosin-binding protein H-like (MYBPHL) gene are linked to hereditary DCM, atrial fibrillation, and atrioventricular arrhythmias. MyBP-HL is a sarcomeric protein that is highly expressed in the atria with only scarce, distinct clusters of MyBP-HL positive cells in the ventricles, mostly in or surrounding the ventricular conduction system (VCS). Constitutive knock-out of MyBP-HL in mice causes atrial dilation, ventricular dysfunction, arrhythmia, and DCM. Whether MyBP-HL plays a developmental role, or if knock-down in adulthood will recapitulate a similar phenotype has yet to be …

The Anti-Inflammatory Properties Of Exopolysaccharide From Bacillus Subtilis, Nikolay Todorov

Master's Theses

Commensal microorganisms can be used therapeutically to ameliorate a variety of diseases. The laboratory of Dr. Katherine L. Knight studies these therapeutic properties in the commensal microorganism known as Bacillus subtilis. Previously, the Knight lab has demonstrated that B. subtilis can protect against colitis caused by Citrobacter rodentium suggesting that B. subtilis possesses anti-inflammatory properties. These properties are specific to the polysaccharide capsule of the microorganism known as exopolysaccharide or EPS. EPS can be produced and purified for study. EPS has been demonstrated to be protective in a variety of diseases such as allergic eosinophilia, sepsis, Graft versus Host disease, …

Development Of Targeted, Statin Loaded Nanoparticle Delivery Systems, Stephanie Judkins

Master's Theses

Statins lower endogenous production of cholesterol through their inhibition of the mevalonate pathway. Lipophilic statins in particular exhibit anti-inflammatory effects at supraphysiological doses by preventing downstream signaling through small molecule GTPases. Interest lies in development of statins as anti-inflammatory therapeutics, however, achieving effective doses locally incurs serious off-target effects. Our goal was to develop and optimize a targeted delivery system to traffic these drugs to inflamed sites and unload the drug at effective concentrations while avoiding systemic exposure. Lipophilic statins (lovastatin, simvastatin, and atorvastatin) were incorporated into polymeric nanoparticles (NPs) due to their hydrophobicity. We have shown that statins released …

Sport And Environmental Issues: Ncaa Division I Student-Athletes’ Personal Experiences, Ivanna M. Anderson-Villela

Master's Theses

While sport ecology is a growing category in sport management literature, there is a lack of research exploring athletes’ experiences regarding environmental issues, and the impacts environmental issues have on student-athletes. The present study qualitatively explored the personal lived experiences of eight NCAA Division I student-athletes regarding environmental issues with the aim of identifying potential influences on values and beliefs through social norms and understanding the student-athlete perspective. Thematic analysis identified 152 raw data themes and 19 higher order themes, which were organized into four central themes: awareness, care and concern from athletic departments, implications, and advocacy. Generally, student-athletes perceived …

A Qualitative Evaluation Of Speech Sound Disorder Treatment Apps For Children, Rachel G. Barnett

Master's Theses

This research examined 20 speech sound disorder (SSD) treatment apps’ features and costs as related to the feature matching process within speech-language therapy. Methods included the development of a 67-item SSD checklist and sorted into 9 categories, including linguistic levels, speech models, software customization, cost, game types, image types, technical elements, feedback types, and engagement and manipulation. Two independent reviewers applied the checklist to randomly selected contemporary apps available on both iPhones and iPads. App cost was capped at $25. Results indicated that app cost was not a good predictor of feature quantity and contemporary apps had some relevant feature …

“Lovely Is The Hillside” An Ecocritical Analysis Of The Narrative Function Of Setting Elements In Njal’S Saga, Michelle Fae Jones

Master's Theses

This paper uses an ecocritical lens to examine the portrayal of Iceland’s physical environment in Njal’s Saga. Drawing on historical and scientific context, this paper applies the ecocritical lens using an interdisciplinary approach to explore how the author crafted a narrative that idealizes Iceland’s Saga Age, selectively highlighting aspects of the environment that align with a vision of the land’s fertility and the people’s honor. This paper seeks to uncover the nuances of the author’s portrayal through a comparative analysis of four English translations of the saga. Applying the ecocritical lens allows for a deeper discussion of the implications for …

Reinforcement Learning Based Planning And Control For Robotic Source Seeking Inspired By Fruit Flies, Gauravkumar Koradiya

Master's Theses

In the exploration of mobile sensing agents such as robots equipped with sensors, these agents have the potential to measure unknown scalar fields, ranging from chemical concentrations to temperature fluctuations. One of the primary interests is the robots’ ability to gravitate towards the peaks or troughs of these fields, a phenomenon termed as “source seeking”. This behavior is invaluable in practical scenarios, like detecting chemical leaks or locating survivors after catastrophes. However, the challenge becomes significantly more pronounced when operating in turbulent flow fields, where the erratic nature of odorant plumes makes predictions and simulations highly unreliable. Interestingly, fruit flies …

Identification Of Bacterial Transcriptomic Signatures In Breast Cancer Tissues Using Publicly Available Datasets, Rasaq Awosemo

Master's Theses

Breast cancer remains a significant global health concern, with increasing evidence suggesting a link between bacterial infections and cancer progression. This study aims to identify bacterial transcriptomic signatures within breast cancer tissues using publicly available datasets. While previous work in our lab has extensively explored the role of Borrelia burgdorferi in cancer, this research broadens the focus to investigate the presence of various bacterial species across different breast cancer samples.

Employing advanced bioinformatics methodologies, including the STAR aligner and MetaPhlAn for metatranscriptomics analysis, we analyzed RNA-sequencing data from the Gene Expression Omnibus (GEO). Our findings reveal diverse bacterial communities within …

Modeling, Simulation, And Experimental Testing Of A Single-Legged Hopper, John Anthony Bennett

Master's Theses

Legged robots are well-suited for navigating unstructured terrain due to their ability to perform agile dynamic motions. Building on the efforts of Cal Poly's Legged Robotics Group, this project focuses on achieving stable forward hopping with a single legged robot.

A comprehensive model of leg hopping is formulated, with leg dynamics during flight and stance phases derived using Euler-Lagrange equations. During the stance phase, a spring-mass system models the leg-ground interaction. The touchdown and lift-off conditions are formulated mathematically, and state mappings between phases are determined via momentum conservation. A two-phase hybrid controller is designed, incorporating trajectory planning during flight …

Investigation Of The Microbiome Of A Recirculating Aquaculture System (Ras) For Crassostrea Virginica Larvae, Isabelle Townsend

Master's Theses

Recirculating Aquaculture Systems (RAS) offer control over physicochemical and biological factors impacting aquaculture success. One understudied facet of RASs is the water microbiome. Oyster larval microbiomes are shaped by their aquatic microbiomes. Understanding the dynamics of and the factors shaping RAS microbiome may provide insights to how the microbiome can be a tool for optimizing larval production. This study’s goals were to investigate the microbiome stability of a RAS rearing oyster larvae across time and space, determine impacts of larval presence on the microbiome, and examine connections between larvae production outcomes and microbiome stability. Water samples were collected weekly from …

Projected Range Shift Of The Atlantic Surfclam, Spisula Solidissima, And The Ocean Quahog, Arctica Islandica, Under Climate-Induced Benthic Warming: Forecasting Fishery Influence In The Mid-Atlantic Bight Throughout The 21st Century, Molly Mae Spencer

Master's Theses

The Atlantic surfclam, Spisula solidissima, and ocean quahog, Arctica islandica, are biomass dominant bivalve species on the eastern North American continental shelf, both supporting lucrative commercial fisheries in the Mid-Atlantic with a combined value of $53.6 million in ex-vessel revenue per year. Rapid climate change is expected to modify the geographic range of these bivalve populations across the Mid-Atlantic Bight (MAB), suggesting transformative consequences for the surfclam fishery. This study forecasted future distributions of the two species from years 2016 – 2095, based on projected bottom water temperatures and a temperature-dependent population dynamics model, to project subsequent fishery-based indicators …

The Effects Of An Acute Dose Of Cannabidiol On Health And Two-Mile Time Trial Performance, Elyssa Bell

Master's Theses

As more studies exploring the therapeutic effects of cannabidiol (CBD) in both clinical and non-clinical populations continue to increase, there is a renewed interest in the ability of CBD to affect performance. In fact, many anecdotal reports in the media and online suggest that CBD may improve performance. Unfortunately, very few studies support these claims and research in the area is limited. Purpose: The purpose of this study is to explore the effects of an acute dose of CBD on anxiety symptoms, GI distress, heart rate variability (HRV), heart rate (HR), blood pressure (BP), blood lactate (BL) rate of perceived …

Contrast As A Governing Principle: Topic Deployment In Selected Sonatas Of Johann Christian Bach, Morgan Hall

Master's Theses

Between the end of the Baroque era and the end of the Classical era, there was a shift in the way music was composed. This shift allowed composers to employ multiple topics in one work as opposed to the single-affect-per-movement that was standard under the Doctrine of Affections. Despite plenty of scholarship about both of the eras, the transition from one to the other has been relatively unexplored. Early musicologist Charles Burney noted in a volume of his General History of Music that there was a composer who was the first to employ “contrast as a governing principle”, and this …

Determining Spatiotemporal Regulation Of Actin Dynamics In Choroid Fissure Closure, Cristal Gonzalez

Master's Theses

There are several stages of development necessary for proper vision. During vertebrate eye development, early eye morphology progresses in three stages: optic cup morphogenesis, basement membrane breakdown, and tissue fusion. Eye development is an intricate collaboration between many cells and requires tissues to migrate and bridge together. The following research investigates the Danio Rerio eye development to understand the molecular regulation of choroid fissure closure (CFC). It is hypothesized that CFC requires temporal and spatial regulation of actin dynamics. We investigate this question using chemical inhibitors, cytochalasin D, an actin treadmilling inhibitor, and NSC23766, a Rac-GEF inhibitor of the actin …