Digital Commons Network^™

The Effects Of Maternal Diet And Energy Demands On Offspring Development, Growth, And Health, Mary C. Wynn

Master's Theses

Maternal programming can have numerous detrimental effects on offspring development, growth, and health. To improve livestock production efficiency, it is necessary to determine the mechanisms that can result in reduced growth, health, and product quality. We hypothesized that 1) restricted- and over-feeding during gestation would inhibit offspring muscle development, and 2) high maternal milk production would affect factors involved in offspring growth and immunity. To investigate the effects of poor maternal nutrition on offspring muscle development and growth, pregnant Western White-faced ewes (n = 82) were randomly assigned to one of three diets at day 30 of gestation through parturition; …

Spatial Variability In Abundance, Detectability And Survival Of White-Tailed Deer Across A Heterogeneous Landscape Of Fear, Jennifer Kilburn

Master's Theses

No abstract provided.

Investigating Perceptual Discrimination Of Timing Cues In Synthetic Vocalizations In Rodents And Humans, Peter Satonick

Master's Theses

Animals' ability to understand and classify speech and non-speech vocalizations is highly robust, in spite of variations in tonal frequency composition (Belin et al., 2008; Shannon et al., 1995). This ability suggests that timing cues within any given sound envelope are important for discriminating such sounds. However, prior studies examining perceptual thresholds for discriminating timing cues often include tonal cues as well. In this study, we synthesized simple white noise sound burst sequences that contain the timing cues present in natural vocalizations sequences, but that are also devoid of underlying tonal cues. We then trained Long Evans rats and Humans …

Light And Electron Microscopy Analysis Of Gold Nanoparticle Penetration In Stiffness Varying 3d Tumor Spheroids, Mathangi Madhavan

Master's Theses

Mechanical properties have always been considered one of the primary biomarkers in distinguishing normal cells from tumor cells. The prime purpose of this thesis was to study and analyze the penetration of gold nanoparticles in different stiffness , same sized and varying time-points 3D tumor spheroids using light and electron microscopy techniques. Recently a lot of attention has been given to the culture of cancer spheroids for applications such as drug delivery, nanoparticles interaction, and penetration. Such analysis requires high-quality imaging modalities like transmission electron microscopy (TEM). In this study, a set of spheroids were cryo-sectioned to study the distribution …

Circulating Heat Shock Protein Responses During Exercise In A Hot And Humid Environment, Virgilio Lopez Iii

Master's Theses

Given that hyperthermia and other exercise-stress induce cellular release of HSPs into circulation, it is important to consider the contribution of circulating HSP concentrations ([HSPs]) on systemic inflammation associated with exertional heat stroke pathophysiology (EHS). We compared the circulating [HSP90alpha], [HSP60], [HSP70], and [HSP27] in two athletic events with high and low incidences of EHS. The aims were to characterize the changes in circulating [HSPs] from pre- to post-event and to elucidate the potential for HSPs as EHS susceptibility biomarkers. Thirty (age: 44±11year; height: 174.89±9.5cm; mass: 74.2±14.05kg; BMI: 24.11±3.13kg × m^-2; %body fat: 19.74±4.03%) and fifty-six participants (age: …

Development Of Tools For A 3d Reconstructed Intracortical Volume Around Microelectrode Arrays, Aparna Nambiar

Master's Theses

Extensive research using penetrating electrodes implanted in the central and peripheral nervous systems has been performed for many decades with significant advances made in recent years. While these penetrating devices provide proximity to individual neurons in-vivo and perform effectively for several months, they are prone to failure over a year and longer. 2D Histology studies using serial tissue sections have been extremely insightful over the years in identifying and quantifying most of these factors such as astrocytic scar formation, microgliosis, and neuronal death, around the implant sites. However, there are significant limitations of 2D histological studies in providing a holistic …

Assessment Of Cranial Neural Crest Cell Differentiation Towards An Osteoblastic Fate, Sean Congdon

Master's Theses

The world health organization estimates that craniofacial defects affect between 2-3% of all live births, with complications ranging from aesthetic issues to severe mental retardation (Marcucio 2015). It is estimated that for a pathology resulting in craniofacial aberrations, each affected individual costs society ~1 million dollars in corrective and palliative care (Singh 2014). Current investigations into genetics underlying these defects have revealed 564 human (HPO 2017) and 3297 murine (MGI 2017) genes thought to be involved in craniofacial pathologies. Facial development requires a population of cells known as Cranial Neural Crest Cells (CNCCs) to properly migrate from the neural plate …

The Effectiveness Of Men's Lacrosse Shoulder Pads In The Attenuation Of Linear Impact Forces 2018, Ryan S. Dambach

Master's Theses

BACKGROUND/PURPOSE: Men’s lacrosse is a sport that is growing in popularity. The mandatory shoulder pads have no limit of use standards and are rarely studied. The purpose of the study was to measure peak impact force (IF) of new and used lacrosse shoulder pads. METHODS: Six lacrosse shoulder pads, three new models (Warrior MPG 8.0 shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) and three used models of the same shoulder pads with at least four seasons of use (Warrior MPG shoulder pad, STX Jolt shoulder pad and Warrior Player’s Club Hitlyte shoulder pad) were …

Integrated Computer Science Unit For Early Exposure To A Diverse Population, Di Hoang

Master's Theses

Reports from major tech companies, such as Airbnb, Facebook, Microsoft, Google, Apple, and Yahoo, indicate that gender diversity and ethnic diversity are lacking in the tech industry [22]. The US Bureau of Labor Statistics projected a 24% increase in the number of software development positions (roughly an increase of 300,000 jobs) between 2016 and 2026 [64]. The main motivation behind this thesis is to expose computer science to young children and encourage teachers to include computer science in their classrooms in the hope of contributing to the current effort of increasing diversity within tech fields in the next decade.

This …

The Conundrum Of Causal Reasoning In Elephants, Beri Brown

Master's Theses

Causal reasoning is marked by the ability to mentally reconstruct the missing part of a sequence in order to reproduce an outcome. While research on causal reasoning has been done with children, the results of the studies have been inconsistent. A standardized paradigm for comparative causal reasoning studies does not exist. Nissani (2006) investigated causal reasoning in a tool-use task with elephants and concluded that elephants were not capable of causal reasoning. The current study, a modified replication, yielded results that were not congruent with Nissani’s (2006) manuscript. Additionally, it was very unlikely that the Nissani (2006) study truly looked …

A Meat Paradox: Media's Role In Mitigating The Omnivore's Dilemma, Karyn Camille Lewis

Master's Theses

The purpose of this research is to identify and understand media’s role in meat consumption and a disassociation of meat and its animal of origin. This study questions consumer behavior based on media portrayals of meat products as well as how consumers perceive these portrayals, meat consumption patterns based on media and family influence, and the types and levels of satisfaction (ex: self-esteem or masculinity) consumers receive from meat products.

A quantitative research approach was proposed for this study. The primary research method was a survey among students, faculty and staff at The University of Southern Mississippi. A total of …

Factors Affecting The Regrowth Of Ilex Glabra In A Routinely Burned Longleaf Forest, Jaybus Price

Master's Theses

This study examines the effects of historical management by use of prescribed fire on Ilex glabra stems/m² and factors affecting the regrowth of I. glabra after a prescribed burn to gain beneficial knowledge for management purposes. Environmental factors and morphological parameters of I. glabra were sampled before and after a prescribed burn of the Longleaf Trace Nature Preserve in September 2016. The study site is located in Lamar County, Mississippi, just west of Hattiesburg, MS. Stem densities of I. glabra were collected once before the prescribed burn and twice after the prescribed burn at 2 month and 9 month …

Life-History Characteristics And Fishery Dynamics Of Red Drum (Sciaenops Ocellatus), In The North-Central Gulf Of Mexico, Corbin Bennetts

Master's Theses

Red Drum (Sciaenops ocellatus) are highly targeted recreationally in the Gulf of Mexico, and support a small commercial fishery in Mississippi. Despite their popularity, the stock is assessed using data limited approaches that necessitate accurate life history information. I estimated the year-specific and year-aggregated escapement rates for the Mississippi stock (years 2004 to 2015), which were sensitive to mortality estimation methods; year-aggregated estimates were 6.9 to 28.2 % depending on the method, but temporal patterns were consistent. I then addressed concerns with previously estimated life-history characteristics by describing the sex-specific growth and reproductive dynamics. The three-parameter von Bertalanffy …

Vocalizations Of North American River Otters (Lontra Canadensis) In Two Human Care Populations, Sarah Walkley

Master's Theses

There is a dearth of information regarding the vocal repertoire of North American river otters (Lontra canadensis). This indicator species is cosmopolitan yet elusive, making recordings methodologically difficult in the wild. Therefore, this exploratory study uses video and audio recordings of two populations of North American river otters in human care to broaden the known vocal repertoire of river otters in various social contexts. The populations consist of a male-female pair at the Stamford Museum and Nature Center and a male-male pair at The Maritime Aquarium at Norwalk. This study is the first to examine the vocalizations produced …

Interplay Of Dgat1, Pdat1 And Dgat2 Enzymes In Plant Triacylglycerol Assembly, Anushobha Regmi

Master's Theses

Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the transesterification of fatty acid from acyl-CoA to diacylglycerol (DAG) forming triacylglycerol (TAG, a.k.a oils and fats). Most plants have at least two unrelated DGAT genes, DGAT1 and DGAT2. Plants predominantly express only one during oil synthesis; the reason, however is not clear. A few studies have indicated that each enzyme prefers DAG and acyl-CoA substrates with different fatty acid compositions. Industrially desirable seed oil composition can be obtained through genetic engineering by replacing the endogenous enzyme with one that has different substrate selectivity. In Arabidopsis thaliana, DGAT1 and another unrelated enzyme PDAT1 are essential …

Why Sing So Many Songs? Testing The Function Of Song Type Repertoires In Rock Wrens Using Playback Experiments And Behavioral Observations, Stephanie Marie Gobert Pitt

Master's Theses

Bird song has been studied extensively as a model of complex animal communication systems because of how various and flexible it is in certain species. Numerous researchers have investigated the evolutionary pressures leading to the elaboration of songs available in a bird’s repertoire and this topic is most often evaluated in light of sexual selection. Intersexual selection mechanisms such as female choice are often presumed to be very important in the elaboration of song, however literature reviews on the topic find no strong correlation between female preference for larger song repertoires and the presence of the characteristic in males across …

Detection, Activity Measurement And Phylogeny Of Ureolytic Bacteria Isolated From Elasmobranch Tissue, Yimu Yang

Master's Theses

Free-ranging marine elasmobranch tissue-associated micro-organisms were cultured from free-ranging Atlantic stingray (Dasyatis sabina) and Atlantic sharpnose sharks (Rhizoprionodon terraenovae). 16S rRNA gene phylogeny indicated bacteria community structure in both elasmobranchs were under phylum Proteobacteria, Firmicutes and Actinobacteria. By conducting split-plot ANOVA, we found the microbial richness is significantly different (P=0.0814) between two superorders of elasmobranch, which may largely due to their preferred habitats and feeding habits. Urease presentence and activity was detected in phylogenetically diverse bacterial strains. Species with high urea-hydrolyzing ability, such as Micrococcus luteus (shark blood isolate: 46.84 mU/mg protein; stingray blood isolate: 24.36 …

Regional Variation In Migratory Behavior And Reproductive Output Of The Gulf Of Mexico Blue Crab Spawning Stock, Adam Kemberling

Master's Theses

The overarching goal of this research was to better understand the structure and boundaries of the blue crab stock(s) in the Gulf of Mexico, focusing on large-scale movements of spawning female crabs. Because spawning location is a primary determinant of larval transport pathways, understanding spawning locations and offshore movements of female blue crabs can inform stock identification and potentially clarify the somewhat conflicting population genetic information available for blue crabs. A large-scale mark-recapture study was conducted through the partnership of state/federal management agencies, the commercial fishing industry, recreational fisherman, tourists, and numerous academic institutions to shed light on the timing …

Linkages Between Marsh Fragmentation, Prey Availability, And Blue Crab (Callinectes Sapidus) Abundance And Mortality, Lennah Shakeri

Master's Theses

Coastal marshes are important nursery habitats for many commercially important invertebrate species, yet these marshes are being lost worldwide at an unprecedented rate due to subsidence, erosion, climate change, and human activity. As marsh is lost, it creates the opportunity for submerged aquatic vegetation (SAV) colonization and establishment in newly created open water areas. Blue crabs, Callinectes sapidus, use both marsh edge and SAV habitat during the juvenile stages and support one of the largest fisheries in the Gulf of Mexico, worth over $70 million in 2015. This thesis studied the linkages between habitat type, SAV cover, benthic prey …

Spatial Variation In Basal Resources And Trophic Position Of Selected Fishes Of The North-Central Gulf Of Mexico, Christopher Fleming

Master's Theses

The North-central Gulf of Mexico is a complex hydrologic environment with freshwater influx that varies on spatial and temporal scales. Freshwater input exerts influence on the isotope values of organisms living in coastal ecosystems. The objectives of this study were to determine relationships between total length and isotope value, estimate basal resource usage and trophic position of target species from Mississippi Sound, reef, and pelagic environments, and identify differences and similarities in spatial patterns of collection. Muscle tissue samples were collected from October 2014 through September 2015. Stable isotope analysis identified a trophic gradient extending from nearshore to offshore, with …

T.S. Eliot, "The Waste Land", And Yoga Philosophy, Jessica Cloud

Master's Theses

While pursuing his graduate studies at Harvard, T.S. Eliot put a year into deep study of the Yoga Sutras with renowned scholar James Haughton Woods. Yoga, defined in the Sutras as the practice of stopping “the fluctuations of the mind-stuff” (Patañjali 8), provides the possibility of hope and equanimity in Eliot’s poem The Waste Land (1922), which depicts a world seemingly devoid of meaning. Not only can the influence of the Yoga Sutras be seen in the poetic form, style, and voice of The Waste Land and in the explanatory notes to the poem provided by Eliot, but classical yoga …

Detecting Speakers In Video Footage, Michael Williams

Master's Theses

Facial recognition is a powerful tool for identifying people visually. Yet, when the end goal is more specific than merely identifying the person in a picture problems can arise. Speaker identification is one such task which expects more predictive power out of a facial recognition system than can be provided on its own. Speaker identification is the task of identifying who is speaking in video not simply who is present in the video. This extra requirement introduces numerous false positives into the facial recognition system largely due to one main scenario. The person speaking is not on camera. This paper …

The Impacts Of Sex And Myogenic Cell Transplantation On Collateral Capillary Arteriogenesis, Padon Mary Sivesind

Master's Theses

Current treatments for peripheral arterial occlusive disease (PAOD) have limited success, so there is a need to develop more effective treatments. Because patients with native collaterals have a better prognosis, promoting collateral arteriogenesis is a potential PAOD treatment. Additionally, female PAOD patients have a worse response to treatment and a worse prognosis compared to males, which could be due to impaired collateralization. Cell transplantation is a potential treatment option to promote collateral arteriogenesis. Bone marrow derived stem cells are the main cell type that has been investigated, but they have had limited clinical success. Delivering a stem cell type native …

Enhancements To The Microbial Source Tracking Process Through The Utilization Of Clustering And K-Nearest Clusters Algorithm, Tram B. Lai

Master's Theses

Bacterial contamination in water sources is a serious health risk and the sources of the bacterial strains must be identified to keep people safe. This project is the result of a collaboration effort at Cal Poly to develop a new library-dependent Microbial Source Tracking method for determining sources of fecal contamination in the environment. The library used in this study is called Cal Poly Library of Pyroprints (CPLOP). The process of building CPLOP requires students to collect fecal samples from a multitude of sources in the San Luis Obispo area. A novel method developed by the biologists at Cal Poly …

Improvement Of Chilling Efficiency And Product Quality Of Broiler Carcasses Using Sub-Zero Saline Solutions For Chilling, Morgan Metheny

Master's Theses

Sub-zero saline solutions were evaluated for the improvement of chilling efficiency and product quality of broiler carcasses. In this study, four experiments were conducted to chill broiler carcasses using different saline solutions and chilling temperatures in the Meat Processing Center at California Polytechnic State University (Cal Poly, San Luis Obispo, CA) or in the processing plant at Foster Farms (Livingston, CA). In Experiment I, three salt concentrations and solution temperatures (0% NaCl/0.5°C, 4% NaCl/-2.41°C, and 8% NaCl/-5.08°C) were used to chill carcasses. The fillets in brine chilling at sub-zero temperatures showed lower shear forces than the fillets in 0% NaCl …

Evaluating Recruitment Seasonality Of Red Abalone (Haliotis Rufescens) To Inform Fisheries Management And Conservation Policy, Leslie Christine Hart

Master's Theses

Recruitment, the addition of new individuals to a population, must be understood to make predictions about population growth of marine invertebrates. Red abalone (Haliotis rufescens) represent a former important commercial fishery in California, and until recently, supported a major recreational fishery. However, there have been statewide declines since the 1960s due to overfishing, disease, and climatic factors. Thus, understanding population dynamics to inform management and population restoration is critical. Recruitment dynamics of red abalone are poorly understood, with no prior knowledge of seasonal trends. To address this knowledge gap, I assessed monthly (July 2016-June 2017) and annual (2012-2016) …

Comparative Proteomics: Assessing The Variation In Molecular Physiology Within The Adductor Muscle Between Mytilus Galloprovincialis And Mytilus Trossulus In Response To Acute Heat Stress, Joshua Scott Mier

Master's Theses

Increases in seawater temperatures have imposed physiological constraints which are partially thought to contribute to recently observed shifts in biogeographic distribution among closely related intertidal ectotherms. For instance, Mytilus galloprovincialis an introduced warm-adapted species from the Mediterranean, has displaced the native cold-adapted congener, M. trossulus, over large latitudinal expanses off the California coast. Several comparative physiological studies have revealed interspecific differences in thermal tolerance, including variation in aerobic metabolism and gape behavior, which suggest the invasive congener is better adapted to acclimate to increasing seawater conditions as predicted due to climate change. However, current analyses seek to discover the …

Nanoscale Mapping Of In-Situ Micro Electro Mechanical Systems With Atomic Force Microscopy, Manuel Rivas

Master's Theses

Micro-electro-mechanical-systems (MEMS) are increasingly at our fingertips. To understand and thereby improve their performance, especially given their ever-decreasing sizes, it is crucial to measure their functionality in-situ. Atomic Force Microscopy (AFM) is well suited for such studies, allowing nanoscale lateral and vertical resolution of static displacements, as well as mapping of the dynamic response of these physically actuating microsystems. In this work, the vibration of a tuning fork based viscosity sensor is mapped and compared to model experiments in air, liquid, and a curing collagen gel. The switching response of a MEMS switch with nanosecond time-scale activation is also monitored …

Implementing Modified Aha Criteria In The Measurement Of Blood Pressure In Uconn’S Migrant Farmworker Clinics, Alexandra Turgeon

Master's Theses

No abstract provided.

Characterizing The Roles Of Staphylococcus Aureus Secreted Factors In Virulence And Modulation Of Innate Immune Cell Activity, Cameron Harvey

Master's Theses

Staphylococcus aureus is a prominent human pathogen that is responsible for a massive burden on healthcare. This thesis takes two approaches to characterize how S. aureus secreted factors subvert the innate immune response. First, characterization of the integral membrane protease 1984 showed that it regulates secretion of a putative lytic transglycosylase, IsaA, which is implicated in virulence. My results suggest IsaA and 1984 are capable of modulating immune responses that result in enhanced heart colonization in vivo. In addition, recent studies have uncovered TLR-independent pathways that induce inflammatory responses after S. aureus insult. Therefore, I hypothesized the bacterium secretes factors …