Digital Commons Network^™

Efficient Synthesis Of Aromatic Quinoxaline Derivatives, Patrick D. Larosa

Honors Theses

Quinoxaline and its derivatives have many antimicrobial properties that make them possible substitutes for current medication, with abilities including antibacterial, anticancer, antiviral, and antifungal among others [1]. It is important to be able to quickly and effectively develop new compounds. Current methods to synthesize quinoxaline derivatives are cumbersome, with long reaction times, low yields, and required solvents that add hazards and costs. Microwave-assisted synthesis is a novel methodology to synthesize quinoxaline derivatives in only 5 minutes with no solvent. This study analyzes microwave irradiation as a synthesis technique.

Identification And Comparison Of Hydrolases Secreted From Naganishia Albida To Papiliotrema Laurentii And Their Activity Toward Natural And Synthetic Polyesters, Clarissa A. Ove

Honors Theses

Esters and amides are the most versatile functional groups in nature and in plastics today. Understanding the key temporal events in the biodegradation and susceptibility of polymers and plastics (e.g. polyester and polyester polyurethanes) to hydrolytic enzymes is important to the sustainable development of our modern society. I will present the isolation, identification, and classification of an environmental strain of Naganishia albida found on polymer coatings inside of aircraft. To better understand the capability of hydrolytic enzymes to degrade plastics we isolated, identified, and classified an environmental strain of Naganishia albida found on polymer coatings inside an aircraft. We utilized …

Competition Versus Choice: Evolution Along A Narrow Path In Drosophila Β2 Tubulin, Olivia B. Parson

Honors Theses

The Drosophila melanogaster β2 protein (Dmβ2) has sustained a long evolutionary stasis for the last 60 million years (Nielsen 2006). Even small changes to the protein’s primary amino acid sequence render it non-functional, suggesting its stasis may be due to stringency in the structure/function relationship (Nielsen 2001). This project seeks to understand what has prevented Dmβ2 from evolving, with the two main hypotheses being that Dmβ2 either exists as an ideal protein configuration that competitively bests all alternates or that Dmβ2 is the only possible configuration that will support spermatogenesis in Drosophila melanogaster. In order to test these hypotheses, the …

A Longitudinal Investigation Of Sibling Effects On Parent-Child Relationships, Caleigh G. Pistorius

Honors Theses

This study examines the relationship between one’s older siblings’ internalizing and externalizing problems and one’s own relationship with one’s parents in early adolescence. Previous research has indicated that sibling relationships are some of the most long-lasting and important relationships in an individual’s life. Family research has also shown that there are situations in which children’s internalizing and externalizing behaviors can shape their relationship with their parents. However, no previous research to my knowledge has explored whether one’s sibling’s behaviors influences one’s own parent-child relationship. During this study, data from the United States Department of Labor and National Longitudinal Survey of …

Measuring Wetland Restoration Success Through Water Quality And Invertebrate Community Indices, Abbey E. Raison

Honors Theses

Wetland restoration projects are essential to preserving these imperiled ecosystems. While restoring lost or degraded wetlands is the first step, determining the success of these restoration efforts is often difficult or only focuses on one aspect of an ecosystem (e.g., water quality testing). I plan to measure the success of wetland restoration through traditional (i.e., water quality testing) and non-traditional methods (i.e., terrestrial and aquatic insect sampling) in native (i.e., control) and restored (i.e., experimental) wetlands in Ohio. I will use sweep nets for aboveground insect collection and a dip net for aquatic macroinvertebrate collection. These samples will be sorted, …

Identifying The Effects Of Anaerobicity And Propionate On Listeria Monocytogenes Metabolism And Central Nervous System Infection, Rebecca Rudd

Honors Theses

Listeria monocytogenes is a facultative foodborne pathogen that can enter the bloodstream and invades the central nervous system to cause meningitis. As an intracellular pathogen, L. monocytogenes replicates inside the host cell cytosol and avoids extracellular immune defenses as it disseminates throughout the body. L. monocytogenes can also reach and cross the blood brain barrier, resulting in severe or fatal symptoms in immunocompromised and elderly patients. The overarching goal of my research project is to better understand how different environmental factors, anaerobicity and propionate, in the intestinal lumen alter the ability of L. monocytogenes to cause infections. In the first …

Investigating The Role Of Neural Stem Cells In Aggressive Gliomas, Sadie Salamone

Honors Theses

In the United States alone, there are more than 1.8 million people diagnosed with cancer every year. This number increases exponentially as the scope is expanded to look at the number of people affected worldwide (National Cancer Institute, 2020). Given that a large number of genetic mutations have been identified, and there is a wide variety of cancers and cancer promoting networks. The current treatments have been extensively researched and explored, but there is ultimately no cure for this aggressive and unrelenting disease. One extremely invasive type of cancer is Glioblastoma Multiforme (GBM), which is a specific type of brain …

Methods For Exploiting High-Resolution Imagery For Deep Learning-Based Diabetic Retinopathy Detection And Grading, Adam M. Saunders

Honors Theses

Diabetic retinopathy is a disease that affects the eyes of people with diabetes, and it can cause blindness. To diagnose diabetic retinopathy, ophthalmologists image the back surface of the inside of the eye, a process referred to as fundus photography. Ophthalmologists must then diagnose and grade the severity of diabetic retinopathy by analyzing details in the image, which can be difficult and time-consuming. Alternatively, due to the availability of labeled datasets containing fundus images with diabetic retinopathy, AI methods like deep learning can provide automated detection and grading algorithms. We show that the resolution of an image has a large …

“Exploring The Advantages And Disadvantages Of Universal Electronic Medical Records”, Zachary Mcclenny

Honors Theses

A universal electronic medical record (EMR) is a platform where all of a patient's medical information, history, and data is stored and maintained by providers over time. A universal platform allows all of the medications, tests, procedures, labs, scans, notes, and any other clinical information for that patient to be kept in a record for any provider to view and make changes to while under their care. Although in the United States today, as well as many other countries, EMRs are owned and operated by privately ran businesses and institutions. Only the private institutions that have access to the EMR …

Use Of Edna To Determine The Presence Of Cryptosporidium Spp. In Freshwater Sources In Harlan, Kentucky, Madison Hall

Honors Theses

Cryptosporidium spp. oocysts are transmitted via a fecal-oral route, e.g., water sources, and the parasite is a leading cause of waterborne disease in the United States. Research into Cryptosporidiosis in rural areas is lacking. This study utilized eDNA methods to determine if Cryptosporidium spp. was present in Harlan, Kentucky, an underserved area in Appalachia. We used a pump-powered commercially available Smith-Root Citizen Scientist eDNA (Vancouver, WA) system to filter freshwater sources and collect eDNA. Samples were collected from tap and spring water as controls and three times from five locations on the three tributaries of the Cumberland River: Martins Fork …

Selective Melt Recrystallization Of Acetaminophen Polymorphs Through Environmental Control, Eleanor Lowe

Honors Theses

Polymorphism is the ability for a molecule to crystallize into multiple different structures in the solid state. The preparation of one polymorph over another is practically important to pharmaceutical development, as properties of polymorphs can vary and impact the drug delivery method. Polymorph outcomes depend heavily on the environment in which the crystal is nucleated, or initially formed. Acetaminophen, the active ingredient in Tylenol, is one example of a polymorphic compound. Here we use acetaminophen as a model system to better understand primary factors controlling polymorph selection and interconversion. We hypothesize a specific atmospheric environmental factor plays large role in …

Long-Range And Chaotic Active Mixing Of Swimming Microbes In A Vortex Chain Flow, Nghia Le

Honors Theses

We present experiments studying the motion and active mixing of swimming mi- crobes in laminar, vortex-dominated fluid flows. We are testing a theory that predicts the existence of swimming invariant manifolds (SwIMs) - invisible, one-way barriers blocking the paths of self-propelled tracers in the flow in one direction. We also pro- pose that the SwIMs together can form chute structures in three-dimensional phase space that facilitate cross-vortex transport of the microbes. We also observe evidence of how these structures promote long-range transport at different non-dimensional velocities (microbe’s velocity relative to flow velocity). Long-range transport is quan- tified by measuring the …

Indigenous Storytelling As Decolonial Praxis, Ceremony And At Colby, Georgia Goodman

Honors Theses

This thesis seeks to amplify Indigenous lifeways, diplomacies, sciences, diplomatic relations, and the power of storytelling. This is not a piece analyzing Indigenous culture. Rather, this thesis returns the gaze to the settler colonial state, specifically its storytelling ideologies, to show that systemic practices of inequity in storytelling can be disrupted and decolonized through a recentering of Indigenous ideologies. For example, reciprocity with lands and animals, reflection on positionality and decentering colonial understandings of time and place.

Does Tssh Deletion In Paraburkholderia Bonniea Affect Its Symbiosis With Dictyostellum Discoideum?, Anna Chen

Honors Theses

Paraburkholderia are amoeba resistant, gram negative bacteria that form facultative symbiotic relationships with D. discoideum, a soil dwelling amoeba host. Three species of Paraburkholderia, P. agricolaris, P. hayleyella, and P. bonniea are able to persistently infect nonnative, or symbiont free, D. discoideum. These three species share a type III secretion system (T3SS) and type VI secretion system (T6SS) that is absent in other close relatives We hypothesized that the ability to persistently infect D. discoideum may be partially attributed to the T3SS and T6SS shared across the three species of Paraburkholderia.

The goal was to test the phenotypic effect of …

Changes In Ecosystem Processes And Functional Traits Over An Elevational Gradient, Tiana Issa

Honors Theses

Elevation gradients have been used to understand how climate change impacts various ecosystems processes by substituting distance across elevation with time, reflecting a previous, colder climate. To monitor the changes in soil organic carbon (SOC), plant community, and functional traits across time in the face of climate change, this study used space-for-time substitution to emulate a long-term study with elevational gradients and revisited the same gradient for nearly two decades to monitor elevational effects across time. With this research, I aim to answer the following questions: (1) How does elevation impact ecosystem processes and alpine plant traits? (2) How does …

Pluralistic Ethical Personalism, Qifan Hu

Honors Theses

In the thesis, I sketch out a general outline for a pluralistic, personalist theory of ethics. This theory intends to capture the phenomenon that our life experience is saturated with ethical and other kinds of values; and it also emphasizes the idea that each ethical agent, or human being, has a unique ethical project that is understandable in light of various ethical values.

The Effect Of Blue Mussel (Mytilus Edulis) Extinction On Ecosystem Function In The Gulf Of Maine Rocky Intertidal, Sophia K. Montague

Honors Theses

Stressors associated with climate change including thermal stress and ocean acidification inhibit the growth and reproduction of many species, including the ecologically and economically important blue mussel species in the Gulf of Maine (GOM). Established scaling relationships between mussel size, abundance, and water filtration suggests that the decline of mussels will stunt GOM water filtration. This change in the rate of water filtration could have large effects on the abundance of phytoplankton, abundance of invertebrate larvae, abundance of nutrients and organic material, food web stability, and biodiversity. Overall, this thesis explores the effect of blue mussel (Mytilus edulis) …

Finding The Common Denominator: Understanding The Shared Experiences Of Female Math Majors, Abigail R. Rosenbaum

Honors Theses

Despite efforts to increase gender diversity in STEM fields, women remain underrepresented in mathematics, especially in advanced academic and research positions. This study aimed to explore the experiences of female math majors as they attempt to navigate this male-dominated space. Through qualitative interviews with seven female math majors, two female math professors, and a focus group with education majors at Woodbridge College, small liberal arts college in the United States, several common themes were identified that define the experiences of female math majors. The findings suggest that math is held at an elevated status in society and that there is …

Tracing North Atlantic Continental Erosion Events During The Mid-Pleistocene Transition, Cecilia T. Bowe

Honors Theses

The Mid-Pleistocene Transition (MPT), which occurred between 1.2 and 0.7 Mya, is characterized by changes in the periodicity and intensity of glacial-interglacial cycles. Early research into this event found that it was not triggered by changes in orbital forcing, but rather by internal climate processes. Previous studies have constrained a probable two-step mechanism, in which erosion of regolith under ice sheets increased glacial volumes and contributed to increased carbon sequestration in the Southern Ocean. Additional research has built on this, suggesting that increased glacial erosion of cratons surrounding the North Atlantic preceded a major weakening of the Atlantic Meridional Overturning …

Testing Evolutionary Conservation Of Sex Determination In Lepidoptera Using Crispr/Cas9 Gene Editing, Sophia Schroeder

Honors Theses

The mechanism of sex determination in Lepidoptera is largely unexplored, and limited to knowledge of only a few genes. Adult Lepidoptera have obvious sexually dimorphic qualities, such as their genitalia. However, significant sex determination genes are not imperative for the adult developmental stage. Knockouts of some sex determination genes (doublesex isoforms) are shown to be lethal in the embryonic and larval stages of development in several animals. However, it is unclear whether this is due to their respective impact on sex determination and sexual dimorphism, or the existence of another function, such as pleiotropy, within a particular gene. Moreover, …

Food As A Vector For Change: Lessons From The Third Sector On Improving Livelihoods With Nutritional Knowledge In Medellín And Bogotá, Solomon Treister

Honors Theses

In this thesis I argue that improving diet in communities depends on building nutritional knowledge. In examining the role of community level organizations, I look specifically at how knowledge is conveyed through agriculture and gastronomy. This project analyzes how civil society organizations work to reintegrate individuals into food systems, compelling consumers to take agency over their diets and pursue better livelihoods. The industrialization of food systems has fundamentally changed the way humans connect with food and diet. In Colombia, internal displacements and urban migration have accelerated a loss of connection with the land and food processes. At the same time, …

Examining The Impact Of Living Arrangements On Children's Academic Achievement, Rachel G. Coan

Honors Theses

In my paper I examine how different living arrangements impact how well a child performs in school. The living arrangements are: living with both biological parents, living with biological mother, and living with biological father. Using a linear regression method, I regress living arrangements against GPA and find that children who do not live with both of their biological parents are expected to perform about 0.3 GPA points worse in school than children who do. With added control variables the difference in predicted GPAs decreases. Additionally, I find that living arrangements aren’t predicted to impact children differently depending on their …

Trends In Cyanotoxin Production Potential In China Lake: An Edna Analysis Of Microcystis And Dolichospermum And The Environmental Drivers Of Cyanotoxin Production, Gennifer M. Rubin

Honors Theses

Episodes of cyano-harmful algal blooms (cyano-HABs) are hypothesized to be exacerbated by the effects of climate change. However, the dynamics of the interactions between elements of climate change and toxigenic cyanobacteria proliferation are not well defined. China Lake is the drinking water source for 7 municipalities in the Kennebec County and is one of many lakes in central Maine that has been subjected to high levels of cyano-HABs and microcystin toxin contamination in recent years. Monitoring the toxicity of these blooms in relation to various aspects of climate change may lead to identification of the major drivers of microcystin production …

Biomechanics Of Mantis Prey Capture, Danielle S. Taylor

Honors Theses

Mantis species have a variety of different morphologies, so do the extreme forms of mantis limbs trade prey catching capability for camouflage? We hypothesize that some extreme forms of mantis limbs that are associated with cryptic species may be associated with a tradeoff of the capability of those limbs. Previous research has developed 2D morphologies of several hundred species of mantises. We are creating a 3D morphology by using micro dissection, micro CT imaging to construct our 3D biomechanical model. We found the attachment points of the ligaments and muscles from a Tenodera forearm and have constructed a 2D biomechanical …

Heading For A Breakdown: Assessing Evolution Through The Hybridization Of Two Sexual Systems, Diamanda A. Zizis

Honors Theses

Hybridization is an important evolutionary pathway that has contributed to the world’s vast biodiversity. In plants, especially angiosperms, hybridization is known to be an important mechanism for speciation, phenotypic divergence, and changes in reproductive systems. Solanum species present an ideal system to investigate how hybridization between two different sexual systems impacts the reproductive and phenotypic biology of the hybrid progeny. Hybrid seeds were acquired from crosses between Australian Solanum species Solanum dioicum (dioecious) and S. ultraspinosum (andromonoecious) in order to track what happens when you cross two plants with different sexual systems. Vegetative and floral morphological measurements were conducted, and …

Particle Swarm Optimization For High Rigidity Spectrometer, Yicheng Wang

Honors Theses

The goal of this project is to find reliable parameter settings for a multi-dimensional global optimizer to optimize the performance of a large acceptance ion optical system for the requirements of nuclear physics experiments. We develop and test the Particle Swarm Optimization (PSO), a global optimization algorithm designed for continuous multi-dimensional problems, on a large acceptance particle beam separator, the High Rigidity Spectrometer (HRS) at the Facility for Rare Isotope Beams (FRIB), which is a laboratory specializing in the production and experimental study of short-lived nuclear matter. We split the HRS into two sections, the High-Transmission Beamline (HTBL) and the …

Cleaning The Variable Mess: A Population Genomics Approach To Understanding The Evolutionary History Of A Complicated Plant Group, Claire Marino

Honors Theses

The Solanum dioicum clade is a group of Australian bush tomatoes consisting primarily of species native to Western Australia and the Northern Territory whose species delimitations tend to be quite unclear. Since David Symon’s monograph A Revision of the Genus Solanum in Australia was published in 1981, little additional progress has been made in clarifying these relationships. In this project, a population genomics approach was used to analyze the relationships and delimitations between three species within the S. dioicum clade: Solanum dioicum, S. carduiforme, and S. ossicruentum. The use of population genomics methods is advantageous to understanding …

A Theoretical And Computational Model For Aquaponics Systems, Isabella M. Digiulio

Honors Theses

Aquaponics, a sustainable farming technique combining the principles of aquaculture and hydroponics, suffers from uncertainty in its optimal operating conditions, preventing large-scale adoption of this technology. Relying on experimentation or full-scale testing to address these uncertainties is an approach that is material, labor, time, and cost intensive. As a result, aquaponics would benefit from a complete computational model of the system. This would allow for information to be gained without long and expensive experiments, but rather with knowledge of system operations and a modeling software package. In this study, the Activated Sludge Model 1 (ASM1) matrix framework, traditionally used in …

Deposited Cigarette Smoke As A Driver Of Oxidation And Partitioning Processes On Indoor Surfaces, April Hurlock

Honors Theses

Despite the large amount of time that individuals spend indoors during their lives, very little attention was paid to the chemistry that occurs on indoor surfaces until recently. Although not visible to the naked eye, physical and chemical processes, such as partitioning and oxidation, are occurring on virtually every indoor surface in a typical household. Indoor pollutants, ranging from skin oils to environmental cigarette smoke, drive a lot of these processes. The transformation of indoor surfaces by indoor pollutants can increase chemical exposure risks as new irritants or carcinogens can be introduced to the surfaces. In this thesis, I describe …

State Level Mental Health Education Compared To Suicide-Related Behavior In Adolescents, 2019, Sonia Rao

Honors Theses

Objective: Determining correlations between state level mental and emotional health (M&E) education and suicide prevention (SP) education to suicide-related behaviors (I.e. feeling sad or hopeless, suicide ideation, making a suicide plan, suicide attempt, suicide attempt treated by medical personnel, and death by suicide) within high school adolescents aged 15-19 within the United States in 2019.

Methods: State level education policies, M&E and SP, were retrieved from the National Association of State Boards of Education (NASBE). Self-reported adolescent suicide related behaviors were retrieved from the 2019 Youth Risk Behavior Surveillance System (YRBSS). Data on deaths by suicide of youth aged 15-19 …