Digital Commons Network^™

The Mystery Of The Epstein-Barr Virus: How To Detect An Undetectable Virus, Paris Lange

Honors Theses

The Epstein-Barr Virus (EBV) is a latent virus that stays in the body after the initial acute infection. In severe cases, this virus can lead to viral myocarditis and can cause life-threatening health issues. One reason this virus is impactful is its ability to suppress the intrinsic apoptotic response through a gene called BHRF1. This gene is an early lytic gene that encodes a homolog of a human pro-survival protein named Bcl-2. This allows the gene to act as an anti-apoptotic survival factor, facilitating the viral persistence inside the host without premature death of the host’s cells. Here, the BHRF1 …

Descriptive Analysis: Effects Of Insufficient Sleep Among College Students Ages 18-22, Divine Ighalo

Honors Theses

Background: Not getting enough sleep is common among college students and is often linked to habits such as screen time, caffeine intake, stress, and irregular routines. This study examined how these lifestyle factors are associated with insufficient sleep among college students ages 18-22 and how poor sleep affects their overall wellbeing. Methods: This study used a cross-sectional survey design and collected data through an anonymous 25 question online Qualtrics questionnaire. This was completed by 150 participants. The survey was designed to be completed in about 5-7 minutes. It included a mix of multiplechoice questions, frequency-based responses and select all that …

From Crops To Kilowatts: The Growing Energy Potential Of Modern Agriculture, Karlee M. Crowe

Honors Theses

Agriculture has traditionally been defined as merely the production of food, fiber, and raw materials. Growing global challenges such as rising energy demand, resource depletion, and environmental degradation are reshaping the agricultural industry. This paper argues that modern agriculture has the potential to expand beyond its conventional roles by integrating renewable energy production—specifically through agrivoltaics and biomass generation—into existing agricultural practices. By focusing on these two main systems, this study highlights how the agriculture industry can implement energy production into already existing practices. Drawing on scientific literature and government sources, this study analyzes the environmental, economic, and social implications of …

Invisible Fallout: Economic Health, Environmental Injustice, And The Social Legacy Of The Paducah Gaseous Diffusion Plant, Isabella G. Bailey-Sims

Honors Theses

This paper examines the long-term impacts of the Paducah Gaseous Diffusion Plant (PGDP) in western Kentucky, framing its legacy as a form of “invisible fallout” in which economic growth was accompanied by delayed environmental and public health consequences. Established in the early 1950s, the plant served as a major economic driver, providing stable employment and transforming the regional economy. However, its operation involved the handling of radioactive materials and hazardous chemicals, leading to environmental contamination and occupational exposure risks that were not fully disclosed during its active years.

Drawing on epidemiological studies, environmental reports, and socioeconomic analyses, this study identifies …

Cancer Risk Perceptions And Nicotine Use Behaviors Among Eastern Kentucky University Students, Kassidy Burke

Honors Theses

The use of electronic nicotine delivery systems (ENDS), including vaping devices and oral nicotine pouches, has increased among adolescents and young adults. Although often marketed as safer alternatives to cigarettes, emerging evidence suggests these products may still pose significant health risks, including potential contributions to cancer. Despite this, many college students perceive them as low risk. This study examines cancer risk perceptions and nicotine use behaviors among students at Eastern Kentucky University, focusing on perceptions of cigarettes, vaping devices, and oral nicotine pouches, as well as how these perceptions influence behavior. It also explores sources of health information and the …

Two Sides Of The Same Cycle: Understanding The Experiences Of Domestic Violence Survivors And Perpetrators, Avery M. Blevins

Honors Theses

Domestic violence has been a prevalent concern throughout society for many years but has recently begun to cumulate awareness from policymakers, people in the community, and individuals in service professions. There have been various changes made to the way that we define and understand domestic violence which has further led to the need to explore and educate on this concern. This paper aims to understand the experiences of both survivors and perpetrators that led to such violent circumstances. This creates a more multifaceted understanding of domestic violence and aids in implementing effective resources to both prevent and intervene in violent …

Comparing The Effectiveness Of Neb Precrtm Repair Mix And Nxgen T4 Dna Ligase For Repairing Degraded Dna For Pcr Forensic Applications, Karolena Zvokel

Honors Theses

This study investigated the comparison between using PreCRTM mix (New England Biolabs) and T4 DNA Ligase (New England Biolabs) to repair degraded forensic whole blood DNA samples for PCR amplification. Degraded DNA presents a significant challenge in forensic STR analysis due to reduced amplification efficiency and allele imbalance. Whole blood samples were extracted, quantified, and artificially degraded using UV exposure. Samples were introduced to multiple treatments, including no damage no repair (NDNR), damage no repair (DNR), PreCRTM repair, T4 DNA ligase treatment, and combined repair approaches. Amplified products were analyzed using capillary electrophoresis, and peak height ratios (PHR) were used …

Inbreeding And The Fall Of The Spanish Habsburg Dynasty, Kira L. Kent

Honors Theses

Inbreeding and the Fall of the Spanish Habsburg Dynasty examines how centuries of consanguineous marriages within the Spanish Habsburg family contributed to the dynasty’s decline and eventual extinction. Focusing on the reign and health of Carlos II of Spain, this study explores how dynastic marriage strategies, which were designed to consolidate political power and preserve royal lineage, ultimately produced severe genetic consequences. This thesis analyzes patterns of intermarriage beginning with Carlos I and continuing through the next four monarchs, highlighting the repeated marriages between close relatives. These practices significantly increased the likelihood of inheriting harmful recessive traits. In addition, this …

Sense Of Belonging And Self-Efficacy Of Women In Stemm At The University Of South Alabama, Emilie Dedeaux

Honors Theses

Despite decades of calls and initiatives to diversify science, technology, engineering, math, and medicine (STEMM) disciplines, females are still more likely to leave STEMM career paths compared to their male peers, which is often depicted as a 'leaky pipeline'. Females in STEMM often report greater social alienation, greater selfdoubt, lower sense of belonging and academic satisfaction due to a mismatch in values in STEMM disciplines. STEMM values have traditionally been normed by cisheteronormative, white, male ideologies . While extensive research has focused on the barriers females experience in STEMM that reduce sense of belonging , less research has focused on …

Targeting The Dorsolateral Prefrontal Cortex With Transcranial Direct Current Stimulation To Improve Depression And Anxiety In Parkinson's Disease, Brock Renicks

Honors Theses

The aim of this study is to observe the outcomes of transcranial direct current stimulation (tDCS) as a treatment for Parkinson’s Disease (PD). This study will primarily look at changes in depression and anxiety, as these symptoms are often comorbid with Parkinson’s Disease. Transcranial direct current stimulation is a non-invasive, very low-intensity form of electrical stimulation of the brain. It is being increasingly used as a form of treatment for Parkinson’s Disease and depression and anxiety, however the literature is ambiguous, showing mixed results throughout various studies, with no standardization of tDCS administration. To determine the effect of tDCS on …

Exploring Social Emotional Learning Competencies In Middle School Theatre Education, Katherine J. Mullins

Honors Theses

Social Emotional Learning (SEL) is a method of education in which students are taught to grow in multifaceted ways. Students learn and grow as more than just students in their respective subjects, they grow in emotional intelligence, empathy, and interpersonal skills as well. SEL is not always easy to incorporate into teaching practices, but it tends to lend itself well to a theatre education setting. In a middle school specific setting, SEL and theatre-based activities yield positive results in student competencies both in and out of the classroom. In this research, through comparative analysis and reflective inquiry, I aim to …

Equal Protection Under The Law: Constitutionality Of Current And Proposed Anti-Lgbtq+ Legislation In Tennessee And Its Potential Impact On Business, Henry J. Kalla

Honors Theses

In recent years, the Tennessee General Assembly has become a battleground of sorts for bills attempting to police the treatment of LGBTQ+ individuals. Multiple laws that impact LGBTQ+ citizens have sparked debate as interested parties on all sides seek to clarify the scope of equal protection, religious freedom, and free expression in this evolving age. Federal law has statutes in place that prohibit discrimination against individuals based on certain protected characteristics, but sexual orientation or transgender status are not explicitly among them. The states have interpreted this ambiguity as providing them the freedom to pass their own civil rights legislation …

Implementation Of A Droplet Interface Lipid Bilayer System For Electrical Characterization, Olivia J. Ziemer

Honors Theses

Lipid bilayers are the fundamental structural framework of cell membranes, providing cells with both protection from the environment and interaction with the surrounding environment. Experimental investigation of lipid bilayers and embedded protein channels often involves the formation of an artificial membrane within an electrical circuit. The focus of this project is to implement and verify the functionality of a lipid bilayer characterization technique known as the droplet interface bilayer (DIB), with emphasis on cost-effective system design. To achieve this, the amplifier and software from the ELEMENTS black lipid membrane (BLM) kit were repurposed to reduce the cost of implementing a …

Transformation Efficiency And Gfp Expression In E. Coli, Jessica H. Hobbs

Honors Theses

Genetic transformation of bacteria is a foundational technique in molecular biology and biotechnology, enabling the introduction and expression of foreign genes for research, industrial, and educational applications. This thesis investigates the transformation efficiency and green fluorescent protein (GFP) expression in Escherichia coli using the pGLO plasmid system, with the primary objective of supporting the development of a graduate-level laboratory course focused on bioprocess engineering and molecular biotechnology. The pGLO plasmid contains a GFP reporter gene regulated by an arabinose-inducible promoter and an ampicillin resistance gene that allows for selective growth of transformed cells. Competent E. coli HB101 K-12 cells were …

Salt-Dependent Purification Of Green Fluorescent Protein Via Hydrophobic Interaction Chromatography, Katie L. Collins

Honors Theses

Green fluorescent protein, GFP, is a widely used fluorescent reporter, and its purification provides a clear model for studying downstream bioprocessing. This thesis investigates how binding-buffer salt strength affects GFP retention and elution during hydrophobic interaction chromatography, HIC, by comparing three conditions (1.0×, 0.8×, and 0.6×). GFP-expressing E. coli were lysed and clarified, and the lysate was purified on HIC columns; collected fractions were evaluated qualitatively under UV illumination to track fluorescence distribution. Results showed a salt-dependent trend in which higher salt improved retention and shifted fluorescence toward later elution fractions, while reduced salt weakened hydrophobic interactions and increased earlier …

Community Resilience And Response To Salinity Gradients In Streams Flowing Into Biscayne Bay, Florida, Sebastian M. Jimenez

Honors Theses

Salinity is a shaping force of aquatic biodiversity, and ongoing climate change and unsustainable water-management practices are causing salinity-induced stress across coastal ecosystems. Meiofauna are small (45-1000 μm), taxonomically diverse aquatic invertebrates that are vital to ecosystems and highly responsive to environmental disturbances, making them ideal for assessing the effects of changing salinities on community structure. This study investigated how meiofaunal biodiversity (ASV richness, Faith’s phylogenetic diversity, and community composition) responded to natural salinity gradients along streams flowing into Biscayne Bay, South Florida, which is a region particularly vulnerable to salinization due to sea-level rise, altered hydrology, and watershed modification. …

Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Honors Theses

This study explores how the tempo of music, measured in beats per minute (BPM), affects dogs' heart rate and oxytocin levels, adding to past research that shows that music affects physiological and behavioral states in dogs. Being able to understand these effects holds practical implications for improving overall well-being in canines in environments such as homes, shelters, and clinical environments. Using a within-subjects design, the dogs were exposed to slow and fast versions of one instrumental track, along with a white noise baseline condition before each randomized condition. Heart rate was recorded continuously throughout the session, and oxytocin saliva samples …

Bottles, Butterflies, And Biomass: Recycled Glass Sand, Microbial Additions, And Herbivory Effects On Milkweed Growth, Kealey Skinner

Honors Theses

Plant-soil-microbe interactions are integral components of ecological networks and drive ecosystem function from the bottom-up. Whereas herbivores regulate ecosystem function from the top-down, having opposing and potentially complementary effects on ecosystem function. My thesis sought to understand how below-ground microbial communities contribute to herbivore preference and plant biomass across varied soil substrates. My thesis asked: how do substrate and soil microbial communities influence herbivore preference and plant biomass accumulation on swamp milkweed (Asclepias incarnata)? To address this question, seedlings of A. incarnata were sown in 2.5 cm wide × 10 cm deep pots filled with either natural beach sand or …

Evaluating Fungal Endophytes Inoculation As A Mechanism For Salt Tolerance In Juncus Roemarianus, Rani Badve

Honors Theses

Salinity stress is increasing in coastal and estuarine ecosystems due to increasing temperatures and the reduction of freshwater inputs via precipitation pattern changes. Elevated salinity can negatively affect plant survival, threatening the stability of coastal wetlands and the ecosystem services they provide. Juncus roemerianus is a common and dominant marsh plant in the Northern Gulf Coast that lives in varying salinities. Growing evidence suggests that root-associated fungal endophytes may contribute to enhanced stress tolerance; however, the response of fungal communities to salinity remains poorly understood.

This study evaluated how salinity influences the abundance and diversity of culturable root-associated fungal communities …

Acute Effects Of The Psychoactive Pollutant Fluoxetine On The Antipredator Behavior Of The Gulf Killifish (Fundulus Grandis), Semyra Reus

Honors Theses

Pharmaceutical contaminants are increasingly prevalent in aquatic environments, yet their sublethal effects on ecologically relevant behaviors remain poorly understood, particularly in estuarine systems. Selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine, are of particular concern due to the conserved role of serotonin in regulating behavior across vertebrates. Here, we investigated how acute exposure to environmentally relevant concentrations of fluoxetine influences anti-predator behavior in Gulf killifish (Fundulus grandis), a key estuarine fish commonly found in saltmarsh habitats. Killifish were exposed to control (0 ng/L), low (17.8 ng/L), and high (596 ng/L) fluoxetine concentrations and subsequently tested in the presence …

Choosing Death: The Moral Legitimacy Of Voluntary Active Euthanasia, Emma Rougeou

Honors Theses

During the last three decades, the issue of physician assisted suicide has captivated the interest of private citizens and state legislatures. Thinking about the matter of end-of-life concerns, particularly surrounding euthanasian (practices that hasten the deaths of suffering terminally ill people) have shifted. Views of private citizens and state-level legislation reflects a “slow shift to more permissive attitudes” (Cohen et al., 2012). Meanwhile, as recently as March 2026, state legislators in conjunction with the NY State Governor, passed a bill legalizing medical aid in dying for terminally ill patients. This thesis is intended to thoughtfully contribute to meaningful discourse on …

Urea-Based Boronium Salts As Antimicrobial Agents, Gabriel Allen Merchant

Honors Theses

Quaternary ammonium compounds (QACs) are widely used in disinfectants due to their ability to inhibit the growth of and eliminate microbes. The potency, broad-spectrum activity, and low cost of QACs have made them a cornerstone of modem hygiene. In recent years, QACs have garnered many health and safety concerns due to their overuse and subsequent accumulation in the environment. The increased use of QACs has further led to an increase in antimicrobial-resistant bacteria, making the synthesis of novel antibacterial compounds vital to combat this growing threat. Recent advances in boronium salts offer a unique alternative to address these adverse effects. …

Spectral Characterization Of Colorectal Cancer Using Excitation-Scanning Hyperspectral Imaging, Natalie Hadad

Honors Theses

Colorectal cancer is one of the most common and deadly cancers worldwide, with a lifetime risk of approximately one in twenty-three for men and one in twenty-five for women. Although early detection significantly improves survival, current diagnostic approaches such as tissue biopsy are invasive, reliant on subjective interpretation, have extended turnaround times, and risk missing flat or depressed lesions that are more difficult to detect than raised polyps. These limitations highlight the need for objective, minimally invasive technologies capable of detecting early tissue changes. This study investigates excitation-scanning hyperspectral imaging (Ex-HSI) as a label-free optical approach for identifying colorectal cancer–associated …

Novel Biomarker Discovery In Multiple Sclerosis, Manisha Gangasani

Honors Theses

Multiple Sclerosis (MS) is a chronic neuroinflammatory disorder affecting the brain, spinal cord, and optic nerve. Patients experience cognitive, motor, autonomic, and emotional symptoms that often overlap with other neurological conditions, making early diagnosis challenging. Current diagnostic criteria require evidence of lesions with dissemination in time and space. Racial disparities in MS outcomes are well documented, with Black individuals often exhibiting greater disease severity, higher relapse rates, and increased disability compared to White individuals. These differences likely reflect a combination of genetic, biological, environmental, and healthcare access factors, underscoring the need to improve early diagnosis and reduce inequities in care. …

Characterization Of Circulating Extracellular Proteome In Plasma Samples Of Triple-Negative Breast Cancer Patients, Brionna Wilson

Honors Theses

Extracellular vesicles (EVs) are membrane-bound vesicles secreted by cells in the body that play an important role in intercellular signaling, immune modulation, and transfer of biomolecules between cells. EV s reflect the physiological state of their cells of origin, so they are being explored as potential noninvasive biomarkers for cancer detection and monitoring. Triple-negative breast cancer (TNBC), an aggressive subtype lacking targeted therapies, is difficult to detect early and currently has no reliable prognostic biomarkers. This study aimed to quantify EV concentrations in the blood serum of TNBC patients (n=40) compared with cancer-free healthy controls (n=35) as well as determine …

Developing A Protocol For Non-Cellular Transplantation Of Donor Mitochondria Into Recipient Cells, Andrew Parton

Honors Theses

Tumor innervation has emerged as a critical feature of cancer progression, with increased nerve density correlating with enhanced aggressiveness, metastatic dissemination, and poor clinical outcome. However, the functional contribution of neurons to tumor biology remains incompletely defined. We recently identified the intercellular transfer of mitochondria from neurons to cancer cells as a mechanism that promotes tumor progression. We therefore hypothesized that disruption of this transfer could attenuate tumor aggressivity. To directly investigate this process, we developed an approach to isolate mitochondria from donor cells and transplant them into recipient cancer cells independently of canonical cell-cell interactions. Transferred mitochondria were tracked …

The Oncoming Light: An Experiential Approach To Psychological Exposition Through Dialogue-Driven Narrative, Benjamin G. Dewitt

Honors Theses

Narrative is a lens through which we understand ourselves, our histories, our futures, and our ties to those around us. Narrative form has undergone vast transformations over time, and in the case of “postmodern” literature, pushes the boundaries of form into the experimental, questioning the truth of knowledge itself. While these experimental works can give rise to uniquely powerful emotional experiences, some components of narrative, when abandoned, may only hinder the immersion of the reader. This thesis evaluates several experimental, dialogue-driven narrative works against these components: linguistic, literal, emotional, and intellectual significance, in a hierarchy of importance as proposed by …

Synthesis Of Novel 1,2,3-Triarylnaphthalenes Via Intermolecular Dehydro-Diels-Alder Reaction, Ethan C. Dailey

Honors Theses

The photo-dehydro-Diels–Alder (PDDA) reaction is a useful method for the construction of substituted naphthalene derivatives from acyclic precursors. While intramolecular variants of this transformation are well established, intermolecular PDDA reactions remain rare due to challenges associated with controlling reactivity under photochemical conditions. Previous studies suggest that electron-withdrawing substituents can promote intermolecular cycloaddition by increasing alkyne polarization and stabilizing reactive intermediates. In this work, the effect of enhanced electron-withdrawing character on intermolecular PDDA reactivity was investigated through the incorporation of a 2,3,5,6-tetrafluoropyridinyl substituent. Corresponding alkyne substrates were synthesized and subjected to photochemical conditions, resulting in the formation of 1,2,3-triarylnaphthalene products. The …

Belonging, Governance, And The Future Of The Nation: Rethinking Citizenship In Tribal Nation Communities, Meadow Carroll

Honors Theses

Tribal citizenship is often framed as a question of identity or legal status, but it functions more fundamentally as a structure of governance that determines who participates in political life and whether nations can sustain themselves over time. This study compares the citizenship frameworks of the Cheyenne and Arapaho Tribes and the Cherokee Nation to examine how different enrollment criteria shape political participation, demographic continuity, and institutional capacity. Drawing on tribal constitutions, enrollment materials, governance records, and voter participation data, the analysis demonstrates that citizenship systems produce measurable differences in how tribal governments function in practice. Systems based on fixed …

Skill Growth And Development In An Honors Experiential Learning Course, Julia D. Lyashevskiy

Honors Theses

Problem-solving skills are crucial for students in both schoolwork and the workforce as they help individuals and groups work through obstacles and come up with solutions to a problem. To help prepare students, the Innovation in Honors Lab at the University of Tennessee at Chattanooga is a hands-on, interdisciplinary course that provides an experiential learning opportunity to practice skills while working directly with community partners to find and solve a local issue. This research tracks the development of six specific skills that students use throughout the class. The skills included communication, creative thinking, decision making, problem solving, conflict resolution, and …